CN205749104U - A kind of simple impression device measuring micron order tissue hardness - Google Patents

Abstract

A kind of simple impression device measuring micron order tissue hardness, effectively solves the problem that can not simultaneously complete in the test of tradition tissue cyto-mechanics performance to make impression and measure impression；Its technical scheme solved is, this utility model includes device frame, it is characterized in that, below device frame, hydraulic micromanipulators is installed, hydraulic micromanipulators includes horizontal displacement mechanism and vertical displacement mechanism, and vertical displacement mechanism is fixedly arranged above operating board, and device frame is arranged above the surface tension tester being positioned at above operating board, surface tension tester is built-in with digital micro-analysis balance, and digital micro-analysis balance is hung with tonometer probe through spring；This utility model simple in construction, simple operation, it is possible to the displacement that the degree of depth of impression is converted into while making impression spring measures, reduces measurement error, improves certainty of measurement, and decrease the operating time, improve the efficiency of test.

Description

A kind of simple impression device measuring micron order tissue hardness

Technical field

This utility model relates to histiocyte mechanics properties testing Instrument technology field, particularly relates to a kind of measurement micron The simple impression device of level tissue hardness.

Background technology

Cyto-mechanics (cell mechanics) is a Disciplinary Frontiers of biomechanics, is also one of tissue engineering Important component part.It relate to cell under mechanical loading effect cell membrane, the deformation of cytoskeleton, elastic constant, viscoelasticity, The research of the mechanical properties such as adhesion, and mechanics factor cell growth, growth, maturation, propagation, old and feeble and dead etc. shadow Ring and Mechanism Study.From the point of view of cyto-mechanics, its research it is crucial that the Mechanical loading mode of cell；So finding properly Cell loading method and cytomorphosis and relevant Biological measurement means be the matter of utmost importance of cyto-mechanics research.

In the patent of Application No. 200620012381.6, disclose a kind of micro-nano-scale mechanical property tester, its Structure is: two-dimentional work bench is made up of a big stroke two-dimensional workbench and one little stroke two-dimensional micromotion workbench, little stroke Micro displacement workbench is arranged on big stroke workbench, and sample is placed on little stroke micro displacement workbench, by big stroke workbench band Dynamic little stroke micro displacement workbench moves between two detection devices, and big stroke workbench is driven by piezoelectric ceramic motor, is leading Move on rail, and detected moving displacement by grating scale.And little stroke micro displacement workbench drives sample in the pressures of two detection devices High-precision little range motion is carried out, in order to fine setting detection position and scanning impression figure, little stroke micro displacement workbench under Tou Flexible hinge is formed, by Piezoelectric Ceramic, capacitive displacement transducer detection displacement by line cutting.

In the patent of Application No. 201520803225.0, disclose a kind of improvement micro-nano-scale Mechanics Performance Testing Instrument, including frame, is respectively in frame both sides the impression preparation facilities supported respectively and impression scanning means, X position by support Move drive mechanism and the displacement work table of the cross layered composition of Y displacement drive mechanism, it is characterised in that: described displacement work table Being arranged in frame, displacement work table is provided with elevating mechanism, and elevating mechanism is provided with rotating mechanism, and rotating mechanism is provided with use In the workbench placing sample.

Impression preparation facilities and impression scanning means are all integrated on an equipment by the tester in the two patent, real The metering system measured after the most first making impression, needs the position of mobile example could realize making pressure in operation once Trace and the operation of measurement impression, can not obtain two results simultaneously.

Utility model content

For above-mentioned situation, for overcoming the defect of prior art, it is micro-that the purpose of this utility model is just to provide a kind of measurement The simple impression device of meter level tissue hardness, effectively solving can not be the completeest in the test of tradition tissue cyto-mechanics performance Become to make impression and the problem measuring impression.

Its technical scheme solved is that this utility model includes device frame, it is characterised in that be provided with liquid below device frame Pressure micromanipulator, hydraulic micromanipulators includes horizontal displacement mechanism and vertical displacement mechanism, solid above vertical displacement mechanism Surely having operating board, device frame to be arranged above the surface tension tester being positioned at above operating board, surface tension tester is built-in There is digital micro-analysis balance, digital micro-analysis balance is hung with tonometer probe through spring.

This utility model simple in construction, simple operation, it is possible to while making impression, the degree of depth of impression is converted into bullet The displacement of spring measures, and reduces measurement error, improves certainty of measurement, and decreases the operating time, improves the efficiency of test.

Accompanying drawing explanation

Fig. 1 is schematic diagram of the present utility model.

Fig. 2 is the schematic diagram of this utility model test operation.

Fig. 3 is the curve chart of this utility model hydraulic pressure trace calibration.

Fig. 4 is that this utility model tests the F obtained_appl-h_indGraph of a relation.

Fig. 5 is that this utility model tests the F obtained_appl-Time graph of a relation.

Detailed description of the invention

Below in conjunction with accompanying drawing, detailed description of the invention of the present utility model is described in further detail.

Being given by Fig. 1 to Fig. 5, this utility model includes device frame 1, it is characterised in that be provided with hydraulic pressure below device frame 1 Micromanipulator 7, hydraulic micromanipulators 7 includes horizontal displacement mechanism 6 and vertical displacement mechanism 5, above vertical displacement mechanism 5 Being fixed with operating board 4, device frame 1 is arranged above the surface tension tester 2 being positioned at above operating board 4, and surface tension is tested Instrument 2 is built-in with digital micro-analysis balance 9, and digital micro-analysis balance 9 is hung with tonometer probe 3 through spring 8.

Described tonometer probe 3 is cylindrical tungsten filament.

Described tonometer 3 radiuses of popping one's head in are 0.255 millimeter.

Described surface tension tester 2 uses the surface tension measuring instrument of langmuir monolayer groove.

Described hydraulic micromanipulators 7 resolution is 40 millimicrons.

This utility model in use,

Just calibrating spring 8 before measuring, the calibration of spring 8 coefficient is by making probe 3 contact incompatible surface (glass cover-slip), uses the accurate translated surface of hydraulic pressure micro-manipulator 7 to replace probe 3, the change of effective measurement result.

During test, sample is placed on the operating board 4 of hydraulic pressure micro-manipulator 7, first freely-suspended probe 3 times Manual positioning, raises operating board 4 and sample with backward probe 3 direction, until sample contacts with probe 3.For macroscopic view sample, sample Contact between product and probe 3 is (generally to be occurred by what consistent measure of the change acted on that the power on repetition impression determines The displacement of 10 μm after the initial change of power), and for the experiment of microcosmic, contact point is debugged to power displacement data. After both contact, the operating board 4 of hydraulic pressure micro-manipulator 7 translates up 3 positions of popping one's head in, thus by sample with continuous print Speed (Fig. 4), or continuous print segmentation impression (Fig. 5).These translations cause the electricity collected by Filmware software kit (Kibron) Pressure drop is low, this software kit can make probe 3 measure the power that arrives reduce (same, the power of sample increases) and pop one's head in 3 to upper Move:

${\mathrm{\Δz}}_{probe}=\frac{F_{appl}}{k_{probe}}$

The displacement of probe 3 is deducted, the impression of the 3 entrance samples that obtain popping one's head in from vertical displacement known to this sample:

h_ind=Δ z_sample-Δz_probe

Soft homogeneous material trace relation of defeating on rigid surface is given by:

$E_{tissue}=\frac{G_{tissue}^{\′}}{2*(1+v_{tissue})}=\frac{F_{appl}^{*}(1-v_{tissue}^2)}{h_{ind}^{*}{k}^{*}2r}$

Wherein r is the radius of penetrator, and is elastic respectively and shearing storage modulus, is Poisson's ratio, for limited sample The Hai Yesi correction factor of this thickness.

Carrying out 7-10 substep impression for macroscopic view sample at each point, the transient change of average indentation force (is ignored initial Excessively upper punch), measured by obtaining.Impression is minimum (Fig. 5) to the change of impression, and by regulating without impression sample, It is likely due to the existence of little impression relative to this thickness of sample (less than 5%).Spatial variability is considered as that material is heterogeneous The performance of property, because the measured value of homogeneous material is the most reproducible.Sample determination or be immersed in saline solution, or It is that (spring constant calibration is at device wet or dry in experiment by saline solution sucking-off to the degree that be enough to keep sample moistening In).

During this utility model can be tested with tests below:

(1) polyacrylamide hydrophilic gel

Using other more ripe methods by comparing the hardness measurement of polyacrylamide hydrophilic gel, this equipment and technology obtain To checking.Polyacrylamide hydrophilic gel is used as bio-chemistry separation instrument for a long time, but elastic to the substrate of dependent cells behavior recently Research, already lead to characterize the adjustment with material behavior widely.The polyacrylamide gel of principal component is retouched according to document State to prepare and cut into zigzag and measure.

(2) integral hardness of micro-object

One unique function of this miniature probe device is to measure the overall mechanical performance of the least biological sample, as The measurement of isolated intact mice glomerule hardness.Glomerule is deposited as dilute suspension on glass cover-slip, by this lid Slide is installed on (use adjutage) hydraulic pressure micro-manipulator.Adjutage is configured to allow for above-mentioned suspension becomes an inversion Microscopical target.Probe is positioned higher than coverslip, is then reduced to glomerule and probe the most visible under 10 × amplification.With Micro-manipulator location glomerule makes it directly can record under probe, turns over so that constant rate of speed is manual-up then towards probe Turn (every five seconds for example 200 nanometer).Hertz model describes the deformation of spheroid in the plane, and is compressed in view of along with sample, contact Area increases:

$F_{appl}=\frac{4\sqrt{R_{sample}}}{3\mathrm{\π}}\left(\frac{E_{tissue}}{1-v_{tissue}^2}\right)h_{ind}^{\frac{3}{2}}$

It is wherein the radius of glomerule, our measurement is approximately 100 microns.

(3) local elasticity of biological tissue samples

Measure with mouse aorta wall tissue slice for sample, the aorta wall thickness microscope by tissue slice Check and obtain.The impression that aorta wall hardness enters continuous 5 microns of sample by probe records, and impression subtracts from common 10 microns Little to 5 microns is the error of the estimated wall thickness that may cause for sample compression.

(4) the whole micron-sized change of tissue hardness

The microscopic dimensions of penetrator can be to the mechanical property of tissue in relevant natural and pathobiology variation Level on measure.In order to demonstrate this function, it is raw that we use the said equipment to be characterized in two kinds of distinct pathology The change of yardstick (the most hundreds of microns to tens millimeter) in the case of reason: the rat Early hepatic fibrosis of carbon tetrachloride process and mice Malignant breast tumor.

Molluscum contagiosum gland is determined in the mechanical property of the different phase of tumour progression.Be applied on sample continuous 10 micro- Total power of meter Wei Yi and stress relaxation degree, can detect that obvious difference between tumor and non-tumor region.

This utility model simple in construction, simple operation, it is possible to while making impression, the degree of depth of impression is converted into bullet The displacement of spring measures, and reduces measurement error, improves certainty of measurement, and decreases the operating time, improves the efficiency of test.

Claims (5)

1. measure a simple impression device for micron order tissue hardness, including device frame (1), it is characterised in that device frame (1) Lower section is provided with hydraulic micromanipulators (7), and hydraulic micromanipulators (7) includes horizontal displacement mechanism (6) and vertical displacement machine Structure (5), vertical displacement mechanism (5) is fixedly arranged above operating board (4), and device frame (1) is arranged above being positioned at operating board (4) top Surface tension tester (2), surface tension tester (2) is built-in with digital micro-analysis balance (9), on digital micro-analysis balance (9) It is hung with tonometer probe (3) through spring (8).

A kind of simple impression device measuring micron order tissue hardness the most according to claim 1, it is characterised in that described Tonometer probe (3) be cylinder tungsten filament.

A kind of simple impression device measuring micron order tissue hardness the most according to claim 1, it is characterised in that described Tonometer probe (3) radius be 0.255 millimeter.

A kind of simple impression device measuring micron order tissue hardness the most according to claim 1, it is characterised in that described Surface tension tester (2) use langmuir monolayer groove surface tension measuring instrument.

A kind of simple impression device measuring micron order tissue hardness the most according to claim 1, it is characterised in that described Hydraulic micromanipulators (7) resolution be 40 millimicrons.