CN204918617U - Cell culture bottle - Google Patents
Cell culture bottle Download PDFInfo
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- CN204918617U CN204918617U CN201520384970.6U CN201520384970U CN204918617U CN 204918617 U CN204918617 U CN 204918617U CN 201520384970 U CN201520384970 U CN 201520384970U CN 204918617 U CN204918617 U CN 204918617U
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- bottle
- tissue culture
- culture flask
- cell culture
- millipore filtration
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Abstract
The utility model discloses a can be applied to the cell culture bottle in bio -pharmaceuticals field, by the glass system can relapse highly compressed bottle, through the external screw thread finish with can relapse highly compressed internal thread bottle lid and be connected and constitute, suitably trompil in the middle of the bottle lid, the hydrophobic millipore filtration of installation in the bottle lid to silica gel sealing gasket with same trompil compresses tightly fixedly. Its technological effect lies in, hydrophobic millipore filtration can the periodic replacement, and glass bottle body can wash repeatedly, and all parts can relapse autoclaving, make cell culture bottle can realize good gas permeability under aseptic condition to can avoid the culture medium excessively to evaporate, cell culture bottle can use repeatedly, greatly reduced the suspended cell culture cost, provide economic cell culture mode for biological drug development and the development of using the suspended cell culture preparation.
Description
Technical field
The utility model relates to a kind of Tissue Culture Flask of bio-pharmaceuticals apparatus field.
Background technology
Tissue Culture Flask is the important consumptive material that bio-pharmaceuticals industry carries out that cell cultures produces medicine, particularly in field of recombinant protein production, to Tissue Culture Flask aseptic, gas permeability requirements is quite high, is thus subject to the extensive favor of pharmaceutical industries and medical device industry researchist.Tissue Culture Flask, can be divided into glass Tissue Culture Flask and plastic cell culture bottle according to material; Circle, square, rectangle, trilateral etc. can be divided into according to culturing bottle bottom shape.Volume according to Tissue Culture Flask can have the plurality of specifications such as 5-1000ml.The attached cell culturing bottle that can be divided into according to Growth of Cells mode and suspending cell culture bottle.
Biopharmaceutical industry develops rapidly, makes mammalian cell suspension culture become the recombinant protein production technology of main flow.Mammalian cell suspension culture uses disposable plastic Tissue Culture Flask usually, because this culturing bottle security is high, full-fledged, bottle cap has sealing cover, ventilating cover etc. available; But the use cost of batch cultur bottle is also very high, not only bring heavy economical load to investigator, also cause the serious wasting of resources in research and production process; And glass Tissue Culture Flask can autoclaving repeatedly, use cost reduces greatly, but glass Tissue Culture Flask only has screw seal lid at present, need sealing cover to unscrew when carrying out cell cultures, stablize with logical the amount and pH that ensure cell culture, such operation is not only inconvenient, and the Pollution risk in culturing process also can be made greatly to increase.
Therefore, need a kind of repeatedly used glass Tissue Culture Flask, it has aseptic venting bottle cap simultaneously, aseptically carries out gaseous interchange to meet in culturing process.
Utility model content
The purpose of this utility model is to overcome the deficiencies in the prior art, provide a kind of can the Tissue Culture Flask of Reusability, it can ensure the gaseous interchange of aseptic condition in cell cultivation process.
A kind of scheme realizing above-mentioned purpose is, a kind of Tissue Culture Flask, comprises bottle and bottle cap, and described bottle cap is connected with bottle by screw socket.
Described bottle cap material is high density polyethylene(HDPE) (HighDensityPolyethylene, referred to as " HDPE "), press bed is utilized to open looping pit at bottle cap center, the disc hydrophobic microporous filter membrane that diameter is identical with bottle cap internal diameter is installed in bottle cap, to provide aseptic gas to exchange for carrying out cultured cells in Tissue Culture Flask, reduce the contaminated risk of cell in Tissue Culture Flask.Described hydrophobic microporous filter membrane can be bought from market, and pore size filter is generally 0.1-0.45 μm, preferably 0.22 μm; Described filter membrane is the hydrophobic filter membrane being usually used in cell open culture, is particluarly suitable for CO
2carry out cell cultures in incubator, be particularly useful for the experiment needing to carry out long term cell culture.
Further, in order to be fixedly mounted on the millipore filtration in bottle cap, using the silica gel sealing pad that diameter is identical with bottle cap internal diameter, stamping out the looping pit of same apertures, for being fixed filter membrane; The available blunt-ended forceps of silica gel sealing pad takes out and puts back to, and is convenient to the replacing of filter membrane.
Further, in order to ensure the ventilation property under aseptic condition, prevent substratum excessive vaporization, need according to the volume of bottle restriction bottle cap perforate size, general opening diameter is the 1/10-1/3 of cap diameter simultaneously, preferably 1/5.
Further, the filter membrane that hydrophobic microporous filter membrane in bottle cap more can renew after high pressure repeatedly 10-20 time is installed on, to ensure the gaseous interchange under aseptic condition.
Further, bottle is made up of glass;
Further, bottle is made up of borosilicate glass.
Again further, bottleneck is outside screw mouth; Bottle cap is correspondingly inner thread mouth.
Again further, described bottle can carry out silanization to reduce cell adsorption.
Have employed the technical scheme of a kind of Tissue Culture Flask of the present utility model, namely glass system can repeatedly high pressure bottle, by external screw thread finish with can the internal thread bottle cap of high pressure repeatedly be connected, suitably perforate in the middle of described bottle cap, the hydrophobic microporous filter membrane in preferably 0.22 μm of aperture is installed in bottle cap, and is fixed with the silica gel sealing pad of same perforate.Therefore, described Tissue Culture Flask aseptically can realize good ventilation property, and substratum excessive vaporization can be avoided, whole Tissue Culture Flask can through thoroughly cleaning, Reusability after autoclaving, greatly reduce suspension cell culture cost, for the bio-pharmaceutical Study and Development using suspension cell culture to prepare provides economic cell cultures pattern.
Accompanying drawing explanation
Fig. 1 is the front view of a kind of Tissue Culture Flask of the present utility model.
Fig. 2 is the building block figure of a kind of Tissue Culture Flask of the present utility model.Wherein
1: body; 2: screw thread bottleneck; 3: perforate screw cap
Fig. 3 is the bottle cap vertical view of a kind of Tissue Culture Flask of the present utility model.
3: perforate screw cap; 4: circular openings gasket; 5: hydrophobic microporous filter membrane.
Fig. 4 is the Cell viability and the cell density figure that use a kind of Tissue Culture Flask of the present utility model to cultivate CHO.Wherein a is the disposable Tissue Culture Flask culturing cell of commercialization; B is culturing cell after the utility model Tissue Culture Flask cleaning and sterilizing 1 time; C is culturing cell after the utility model Tissue Culture Flask cleaning and sterilizing 10 times.
embodiment:
Refer to Fig. 1, Fig. 2 and Fig. 3, contriver of the present utility model, in order to set forth the technical solution of the utility model better, below by specific embodiment, and is described in detail by reference to the accompanying drawings.
Refer to Fig. 1 and Fig. 2, a kind of Tissue Culture Flask (Fig. 1) of the present utility model, comprises bottle 1 and external screw thread finish 2, special internal thread bottle cap 3, bottle cap 3 is by screw thread and bottleneck 2 compact siro spinning technology (Fig. 2), and structure aseptic condition prevents cultured cells in Tissue Culture Flask from being polluted.
There is circular openings (Fig. 3) at the center of bottle cap 3, inner installation hydrophobic microporous filter membrane, and is fixed with the silica gel sealing pad of same perforate.The object of such design is, both the ventilation property of bottle cap had been ensured, aseptic condition can be created again, and prevent substratum from evaporating, simultaneously by cleaning, change after hydrophobic microporous filter membrane through autoclaving, realize the repeatedly used object of Tissue Culture Flask, greatly reduce the cost of suspension cell culture, reduce the resource loss of bio-pharmaceuticals research and development.
In the present embodiment, not isometric Tissue Culture Flask, is equipped with the bottle cap (Fig. 2) of different diameter, and the object of design is like this to ensure the gaseous interchange with outside atmosphere in Tissue Culture Flask, is particularly suitable at CO
2cell cultures is carried out in incubator.
In the present embodiment, adopt through cleaning and autoclaved described Tissue Culture Flask, at CO
2cultivate Chinese hamster ovary celI in incubator, temperature controls at 37 DEG C, CO
2institute's concentration is 5%, and shaking speed is 120rpm, and initial cell density is 3 × 10
5cells/ml; Sampling in every 24 hours, carries out cell counting and motility rate measures, and as shown in Figure 4, Cell viability all remains on more than 95% to result, and also disposable Tissue Culture Flask is suitable with commercialization for cell growth curve, confirms that the design of described Tissue Culture Flask is reasonable.
Claims (9)
1. a Tissue Culture Flask, comprise bottle, bottleneck and bottle cap, described bottle cap is connected with described bottle by the screw thread of bottleneck, it is characterized in that, described bottle cap designs for perforate, and opening diameter is the 1/10-1/3 of cap diameter, installs the size millipore filtration identical with cap diameter in bottle cap, described bottle is made up of glass, and the blade diameter length ratio of bottle is 2:1 ~ 1:1.
2. Tissue Culture Flask according to claim 1, is characterized in that the material of described bottle cap is high density polyethylene(HDPE), centered by open looping pit design.
3. Tissue Culture Flask according to claim 1, is characterized in that opening diameter is 1/5 of cap diameter.
4. Tissue Culture Flask according to claim 1, is characterized in that, the pore size filter of millipore filtration is 0.1 ~ 0.45 μm.
5. Tissue Culture Flask according to claim 4, is characterized in that, the pore size filter of millipore filtration is 0.22 μm.
6. the Tissue Culture Flask described in claim 4 or 5, is characterized in that, described millipore filtration is fixed with the silica gel sealing ring of same perforate.
7. Tissue Culture Flask according to claim 6, is characterized in that, millipore filtration installs 1-3 layer and filter membrane can arbitrarily be changed.
8. Tissue Culture Flask according to claim 1, is characterized in that described bottle is made up of borosilicate glass.
9. Tissue Culture Flask according to claim 1, is characterized in that: the blade diameter length ratio of described bottle is 3:2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520384970.6U CN204918617U (en) | 2015-06-05 | 2015-06-05 | Cell culture bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520384970.6U CN204918617U (en) | 2015-06-05 | 2015-06-05 | Cell culture bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204918617U true CN204918617U (en) | 2015-12-30 |
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ID=54967763
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520384970.6U Active CN204918617U (en) | 2015-06-05 | 2015-06-05 | Cell culture bottle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204918617U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106244434A (en) * | 2016-09-27 | 2016-12-21 | 新疆惠森生物技术有限公司 | A kind of classification inoculation apparatus being applicable to microbial manure liquid fermentation and making and application |
| CN107460125A (en) * | 2017-10-12 | 2017-12-12 | 李慧杰 | Suspending cell culture bottle |
-
2015
- 2015-06-05 CN CN201520384970.6U patent/CN204918617U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106244434A (en) * | 2016-09-27 | 2016-12-21 | 新疆惠森生物技术有限公司 | A kind of classification inoculation apparatus being applicable to microbial manure liquid fermentation and making and application |
| CN106244434B (en) * | 2016-09-27 | 2018-09-28 | 新疆惠森生物技术有限公司 | It is a kind of suitable for the classification inoculation apparatus of microbial manure liquid fermentation and making and application |
| CN107460125A (en) * | 2017-10-12 | 2017-12-12 | 李慧杰 | Suspending cell culture bottle |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20180717 Address after: 102206 room 1322, 1 building, 33 Science Park Road, Changping District science and Technology Park, Beijing. Patentee after: Military science is the source of drug research (Beijing) Co. Ltd. Address before: Room 1218, Building 1, 33 Science Park Road, Changping District, Beijing Patentee before: Biousing Biotechnology Co., Ltd. |