CN204710358U - A kind of micro-fluidic chip - Google Patents
A kind of micro-fluidic chip Download PDFInfo
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- CN204710358U CN204710358U CN201520051517.3U CN201520051517U CN204710358U CN 204710358 U CN204710358 U CN 204710358U CN 201520051517 U CN201520051517 U CN 201520051517U CN 204710358 U CN204710358 U CN 204710358U
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- 230000002209 hydrophobic effect Effects 0.000 claims abstract description 26
- -1 polytetrafluoroethylene Polymers 0.000 claims description 13
- 239000011248 coating agent Substances 0.000 claims description 9
- 238000000576 coating method Methods 0.000 claims description 9
- 239000000463 material Substances 0.000 claims description 9
- 239000004743 Polypropylene Substances 0.000 claims description 7
- 229920001155 polypropylene Polymers 0.000 claims description 7
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims description 6
- 239000004810 polytetrafluoroethylene Substances 0.000 claims description 6
- TXEYQDLBPFQVAA-UHFFFAOYSA-N tetrafluoromethane Chemical compound FC(F)(F)F TXEYQDLBPFQVAA-UHFFFAOYSA-N 0.000 claims description 3
- 238000002454 metastable transfer emission spectrometry Methods 0.000 claims description 2
- CPUDPFPXCZDNGI-UHFFFAOYSA-N triethoxy(methyl)silane Chemical compound CCO[Si](C)(OCC)OCC CPUDPFPXCZDNGI-UHFFFAOYSA-N 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 13
- 239000007788 liquid Substances 0.000 abstract description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 29
- 239000012528 membrane Substances 0.000 description 11
- 238000002347 injection Methods 0.000 description 7
- 239000007924 injection Substances 0.000 description 7
- 230000000694 effects Effects 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 6
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 6
- 238000010586 diagram Methods 0.000 description 4
- 229920002521 macromolecule Polymers 0.000 description 4
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 4
- 239000004926 polymethyl methacrylate Substances 0.000 description 4
- 229940058401 polytetrafluoroethylene Drugs 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 239000012510 hollow fiber Substances 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 239000002033 PVDF binder Substances 0.000 description 2
- 238000004026 adhesive bonding Methods 0.000 description 2
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- 238000001311 chemical methods and process Methods 0.000 description 2
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- 150000001875 compounds Chemical class 0.000 description 2
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- 239000004205 dimethyl polysiloxane Substances 0.000 description 2
- 235000013870 dimethyl polysiloxane Nutrition 0.000 description 2
- 229920001971 elastomer Polymers 0.000 description 2
- 238000001746 injection moulding Methods 0.000 description 2
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 description 2
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 description 2
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 2
- 238000004381 surface treatment Methods 0.000 description 2
- 102000029749 Microtubule Human genes 0.000 description 1
- 108091022875 Microtubule Proteins 0.000 description 1
- 239000004372 Polyvinyl alcohol Substances 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
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- 229910000323 aluminium silicate Inorganic materials 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
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- 238000005842 biochemical reaction Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
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- 238000010147 laser engraving Methods 0.000 description 1
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- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 229920002379 silicone rubber Polymers 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
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Abstract
The utility model discloses a kind of micro-fluidic chip.The micro-fluidic chip that the utility model provides, comprise egative film and cover plate, chip is positioned on egative film, described egative film and described cover plate sealing-in are integrated, it is characterized in that: described chip comprises at least 1 micro-pond, described micro-pond is communicated with at least 1 steam vent with at least 1 sample intake passage, and there is hydrophobic, air-permeability medium on the inside of described steam vent or surface.The advantage of chip of the present utility model has: reliability and durability, and liquid is without spilling; Easy batch micro operations, cheap; Assist without the need to equipment in use procedure, user operation is convenient.
Description
Technical field
The utility model relates to a kind of micro-fluidic chip, belongs to micro-fluidic chip field and field of biological detection.
Background technology
Micro-fluidic chip is based on micro electronmechanical process technology, forms network by microtubule road on chip, runs through whole system and complete a kind of technology of various biological and chemical process with controlled microfluid.Early stage in microfluidic chip technology development, chip capillary cataphoresis is its mainstream technology, and chip used structure is simple, function singleness; In recent years, micro-fluidic chip starts to functionalization, integrated direction develop rapidly, the important biological and chemical processes such as such as nucleic acid amplification reaction, immune response, lysis become new focus, and in order to study the biochemical reaction of these complexity, usual needs make a large amount of, homogeneous micro-pond on chip, and this together constitutes microreactor array in pond slightly.
Build microreactor array to need to distribute reagent to form a large amount of, homogeneous micro-pond.The current reagent method of salary distribution is varied, as vacuum negative pressure type (CN101590389A), centrifugal (US6627159, US20050199500A1, US2004120856A1, US6919058B2, US20030166265A1, WO9533986A1), and CN102886280A and CN104226385A etc.
Vacuum negative pressure type make use of the air drawing characteristics of the silicon rubber such as PDMS, and reagent is inhaled into each micro-pond.The method defect is that PDMS cannot produce in injection moulding, is difficult to batch micro operations, and with high costs.Centrifugal is utilize centrifugal force to be distributed by the reagent in main channel to enter each micro-pond, and the defect of the method centrifugally usually needs centrifuge, and equipment is heavy, cannot be portable.
In a word, these chips or cannot produce in batches, or need professional and complete equipment, be all not suitable for actual use.
Summary of the invention
A kind of micro-fluidic chip that the utility model provides, assists without the need to equipment, only needs user's simple operations, and reagent can distribute formation multiple homogeneous micro-pond, is applicable to very much actual use.
An object of the present utility model is to provide a kind of new microfluidic chip.
The micro-fluidic chip that the utility model provides, comprise egative film and cover plate, chip is positioned on egative film, described egative film and described cover plate sealing-in are integrated, described chip comprises at least 1 micro-pond, described micro-pond is communicated with at least 1 sample intake passage, and described micro-pond is communicated with at least 1 steam vent, and there is hydrophobic, air-permeability medium on the inside of described steam vent or surface.
In above-mentioned micro-fluidic chip, the material of described hydrophobic, air-permeability medium is polytetrafluoroethylene (PTFE) (PTFE), Kynoar (PVDF), polypropylene (PP) or other compounds.
In above-mentioned micro-fluidic chip, the pattern of described hydrophobic, air-permeability medium is film, column plug, coating.When hydrophobic, air-permeability medium is film, be positioned at upper surface or the lower surface of steam vent, fix by the mode such as gluing; When hydrophobic, air-permeability medium is column plug, be positioned at the inside of steam vent, the mode such as to inlay by physics, gluing is fixed; When hydrophobic, air-permeability medium is coating, be positioned at the inwall of steam vent.
Have air-vent beyond the Great Wall at described film or column, the aperture in described hole is 1nm-1mm.Described hole can be that material inherently has, and also can be to be obtained by laser boring, Heavy ion tracks.
Described coating can be silylating reagent, as hexamethyldisilane amine, MTES, can be also other compounds, as carbon tetrafluoride; The generation type of coating can be coating, plasma treatment, chemical vapour deposition (CVD), sol-gal process.The thickness of described coating is 0.1nm-10 μm.
In above-mentioned micro-fluidic chip, the effect of described sample intake passage stops the diffuse pollution between each micro-pond, namely avoids the molecule in micro-pond or cellular invasion to adjacent micro-pond.In some cases, sample intake passage also can be cancelled.
In above-mentioned micro-fluidic chip, in some cases, described steam vent and described micro-pond are all on same egative film or cover plate, and described steam vent is through hole.Steam vent and micro-pond are can single injection-molded in the advantage of the same side, and can not produce error when egative film and cover plate sealing-in.In addition, if steam vent and micro-pond are not on same egative film or cover plate, hydrophobic permeable membrane is between egative film and cover plate, then be easy between each micro-pond be communicated with, this is because hydrophobic permeable membrane has certain thickness usually, after chip sealing, reagent along the edge flowing of hydrophobic permeable membrane, can cause the connection between each micro-pond.
In above-mentioned micro-fluidic chip, in some cases, each described sample intake passage is communicated with main channel.Such reagent can enter each sample intake passage by described main channel, and then enters each micro-pond.
In above-mentioned micro-fluidic chip, each described micro-pond is directly communicated with described steam vent or is communicated with by interface channel, and the effect of interface channel forms capillary valve in some cases with barrier liquid, or makes steam vent away from micro-pond to facilitate optical detection.
Above-mentioned egative film and cover plate fit tightly; The structures such as above-mentioned micro-pond, sample intake passage, steam vent, main channel, sample intake passage can be arranged on egative film and also can be arranged on cover plate; As long as be arranged on the arbitrary one side in the surface that egative film and cover plate contact; A part is respectively set in upper and lower surface also passable.
In above-mentioned micro-fluidic chip, the spacing in each described micro-pond is equal or not etc.; The equal and opposite in direction in each described micro-pond or not etc.; In the preparation of chip, size and the spacing in micro-pond can be designed as required.
In above-mentioned micro-fluidic chip, the material of described egative film and cover plate is macromolecular compound, metal, glass, quartz, silicon, pottery, macromolecular compound, rubber and alumino-silicate compound; Wherein, macromolecular compound is Merlon, polypropylene or polyvinyl alcohol.
In use, reagent enters described micro-pond to above-mentioned micro-fluidic chip under malleation or suction function, and the air in micro-pond is discharged from steam vent, the hydrophobic, air-permeability dielectric impedance that reagent is inner or surperficial by steam vent, until reagent is full of micro-pond.The advantage of this chip is:
(1) reliable.Because there is the barrier effect of hydrophobic, air-permeability medium, reagent can not spill.In practical operation, closing of the reagent meeting extreme influence injection port spilt, makes reaction normally to carry out.
(2) batch production is applicable to.Egative film and the cover plate of this chip are preferably macromolecular compound, can carry out batch micro operations by injection moulding; Hydrophobic, air-permeability medium such as poly tetrafluoroethylene, polypropylene hollow fiber, plasma processor that this chip uses are common materials and equipment, low price, can by mode batch micro operations such as cuttings.
(3) user operation is convenient.User uses common pipettor just can application of sample, without equipment such as centrifuges.
Accompanying drawing explanation
Fig. 1 is the micro-fluidic chip schematic diagram in embodiment 1.
Fig. 2 is the micro-fluidic chip schematic diagram in embodiment 2.
Fig. 3 is the micro-fluidic chip schematic diagram in embodiment 3.
Fig. 4 is micro-fluidic chip schematic diagram after the sealing-in in embodiment 3.
Wherein, description of reference numerals is as follows:
101 micro-ponds; 102 sample intake passages; 103 steam vents; 104 hydrophobic, air-permeability media; 201 main channels; 301 interface channels.
Detailed description of the invention
The experimental technique used in following embodiment if no special instructions, is conventional method.
Material used in following embodiment, reagent etc., if no special instructions, all can obtain from commercial channels.
In following embodiment, chip manufacturing technology and using method are routine techniques and the method for micro-fluidic chip field and field of biological detection.
Below in conjunction with specific embodiments and the drawings, the utility model is described in further detail, but content of the present utility model is not limited to embodiment.
Embodiment 1.
The micro-fluidic chip of the present embodiment comprises egative film and cover plate is two-layer, and the PMMA film of egative film to be thickness be 0.1mm is thickness is the PMMA sheet material (as shown in Figure 1) of 2mm.Cover plate has the structure such as passage, micro-pond, non-structure on egative film, chip is positioned on egative film.Chip makes by prior aries such as laser engraving, machining or hot-press sealings, and egative film and cover plate are connect by rubber seal and be integrated.
Chip comprises 8 modules, and each module comprises 1 micro-pond 101, and each micro-pond 101 is connected with 1 steam vent 103 with 1 sample intake passage 102.Micro-pond 101 is approximately rectangle, dark 1mm, volume 8 μ L; Sample intake passage 102 degree of depth is 0.5mm, and width is 0.5mm, and length is 10mm; Steam vent 103, on cover plate, is through hole.
In this chip, hydrophobic, air-permeability medium 104 is polyvinylidene fluoride film (pvdf membrane, purchased from Millipore company, thickness 0.2mm, 0.22 μm, aperture).In order to hold this film, being provided with the rectangular recess of correspondingly-sized in the upside of cover plate, with double faced adhesive tape, pvdf membrane being attached in rectangular recess.
It should be noted that: if steam vent 103 is on egative film, hydrophobic, air-permeability medium 104 is between egative film and cover plate, then each micro-pond 101 is easy to lose independence.This is because pvdf membrane has certain thickness, after chip sealing, reagent along the edge flowing of pvdf membrane, can cause the connection between each micro-pond 101.
The using method of this chip is: reagent joins in sample intake passage 102 by pipettor hand-manipulated, and application of sample amount is 10 μ L.Reagent can enter micro-pond 101, and enters steam vent 103, is stopped by hydrophobic, air-permeability medium 104.Then with sealed membrane, steam vent 103 and injection port are sealed.
Test agent is SDS solution (10%W/V), and after tested, in each micro-pond 101, reagent is all full of, bubble-free, and volume is homogeneous, and steam vent place does not have reagent to overflow.
Embodiment 2.
As shown in Figure 2, the micro-fluidic chip of the present embodiment is similar to Example 1, and just the structure of cover plate is different.
The each sample intake passage 102 of cover plate is all communicated with main channel 201, main channel 201 has 1 injection port.
In this chip, hydrophobic, air-permeability medium 104 is polypropylene hollow fiber plunger, is entwined by polypropylene hollow fiber membrane, and this plunger 104 is stuffed in steam vent 103.
The using method of this chip is: reagent joins in main channel 201 by injection port by pipettor hand-manipulated, and application of sample amount is 90 μ L.Reagent can enter each micro-pond 101, and enters steam vent 103, is stopped by hydrophobic, air-permeability medium 104.Then with sealed membrane, steam vent 103 and injection port are sealed.
The effect of sample intake passage 102 prevents the diffuse pollution between each micro-pond 101, can ensure in 90 minutes, and the Small molecular in micro-pond can not diffuse to adjacent micro-pond.
Test agent is urine, and after tested, in each micro-pond 101, reagent is all full of, and volume is homogeneous, and steam vent place does not have reagent to overflow.Place without diffuse pollution after 90 minutes, homogeneity and the independence in each micro-pond are all guaranteed.
Embodiment 3.
The micro-fluidic chip of the present embodiment comprises two-layer, the PMMA sheet material of egative film to be thickness be 1mm, the PMMA sheet material (as shown in Figure 3) of cover plate to be thickness be 2mm.Cover plate there is the structure such as passage, micro-pond, egative film has through hole.Process identical with embodiment 1 with sealing-in mode.
Chip comprises 3 modules, and each module comprises 1 micro-pond 101, and micro-pond 101 is communicated with 2 sample intake passages 102, and micro-pond 101 is communicated with 2 steam vents 103 by 2 interface channels 301.Each sample intake passage 102 is all communicated with main channel 201.Micro-pond 101 is rectangle, dark 1mm, volume 100 μ L; Sample intake passage 102 degree of depth is 0.5mm, and width is 0.5mm; Steam vent 103, on egative film, is through hole.Injection port, also on egative film, is through hole, is communicated with main channel 201.
In this chip, hydrophobic, air-permeability medium 104 is the hydrophobic nano coating (plasma apparatus model is promise letter AP-600, and gas is carbon tetrafluoride) of Surface Treatment with Plasma.Whole egative film is through Surface Treatment with Plasma, and comprise the surface of steam vent 103 inwall all in hydrophobicity, contact angle is 110 °.
The using method of this chip is: by the chip upside down (as shown in Figure 4) after sealing-in, reagent joins in main channel 201 by pipettor hand-manipulated, and application of sample amount is 360 μ L.Reagent can enter micro-pond 101, and enters steam vent 103 by interface channel 301, is stopped by hydrophobic, air-permeability medium 104.Then with sealed membrane, steam vent 103 and injection port are sealed.
Test agent is blood, and after tested, in each micro-pond 101, reagent is all full of, bubble-free, and volume is homogeneous, and steam vent place does not have reagent to overflow.
In this chip, the effect of multiple sample intake passage 102 is that guarantee reagent evenly can be full of micro-pond 101; The effect of interface channel 301 makes to form hydrophobic capillary valve at the junctional interfaces place of interface channel 205 and steam vent 103, if do not have interface channel 205, then reagent is easy to enter steam vent 103, and then overflow.
Above content is in conjunction with concrete preferred embodiment further detailed description of the utility model, can not assert that concrete enforcement of the present utility model is confined to these explanations.For the utility model person of an ordinary skill in the technical field, without departing from the concept of the premise utility, some simple deduction or replace can also be made, all should be considered as belonging to protection domain of the present utility model.
Claims (10)
1. a micro-fluidic chip, comprise egative film and cover plate, chip is positioned on egative film, described egative film and described cover plate sealing-in are integrated, it is characterized in that: described chip comprises at least 1 micro-pond, described micro-pond is communicated with at least 1 sample intake passage, and described micro-pond is communicated with at least 1 steam vent, and there is hydrophobic, air-permeability medium on the inside of described steam vent or surface.
2. a kind of micro-fluidic chip according to claim 1, is characterized in that: the material of described hydrophobic, air-permeability medium is polytetrafluoroethylene (PTFE), Kynoar or polypropylene.
3. a kind of micro-fluidic chip according to claim 1, is characterized in that: the pattern of described hydrophobic, air-permeability medium is film, column plug or coating.
4. a kind of micro-fluidic chip according to claim 3, is characterized in that: described film or column are porose beyond the Great Wall, and the aperture in described hole is 1nm-1mm.
5. a kind of micro-fluidic chip according to claim 3, is characterized in that: described coating is hexamethyldisilane amine, MTES or carbon tetrafluoride.
6. a kind of micro-fluidic chip according to claim 5, is characterized in that: the thickness of described coating is 0.1nm-10 μm.
7. a kind of micro-fluidic chip according to claim 1, is characterized in that: described steam vent and described micro-pond are all on same egative film or cover plate.
8. a kind of micro-fluidic chip according to claim 7, is characterized in that: described steam vent is through hole.
9. a kind of micro-fluidic chip according to claim 1, is characterized in that: each described sample intake passage is communicated with main channel.
10. the micro-fluidic chip according to claim arbitrary in claim 1-9, is characterized in that: each described micro-pond is communicated with by interface channel with described steam vent.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201520051517.3U CN204710358U (en) | 2015-01-23 | 2015-01-23 | A kind of micro-fluidic chip |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201520051517.3U CN204710358U (en) | 2015-01-23 | 2015-01-23 | A kind of micro-fluidic chip |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105483293A (en) * | 2016-01-29 | 2016-04-13 | 中国人民解放军疾病预防控制所 | Fulminating-infectious-disease pathogen detecting primer pair and kit |
| CN108855264A (en) * | 2018-07-12 | 2018-11-23 | 北京乐普智慧医疗科技有限公司 | A kind of multipurpose multi objective micro-fluidic chip |
| CN109030844A (en) * | 2018-07-05 | 2018-12-18 | 领航基因科技（杭州）有限公司 | Automatically generating device and method applied to biosystem microfluid |
| CN111683751A (en) * | 2018-01-22 | 2020-09-18 | Q-莱纳公司 | Sample rack |
| CN113134400A (en) * | 2021-05-22 | 2021-07-20 | 杭州霆科生物科技有限公司 | Micro-fluidic chip capable of removing bubbles |
| CN114160223A (en) * | 2021-12-28 | 2022-03-11 | 北京梓晶生物科技有限公司 | Micro-fluidic chip |
-
2015
- 2015-01-23 CN CN201520051517.3U patent/CN204710358U/en active Active
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105483293A (en) * | 2016-01-29 | 2016-04-13 | 中国人民解放军疾病预防控制所 | Fulminating-infectious-disease pathogen detecting primer pair and kit |
| CN105483293B (en) * | 2016-01-29 | 2019-07-16 | 中国人民解放军疾病预防控制所 | Deadly infectious disease pathogen detection primer sets and kit |
| CN111683751A (en) * | 2018-01-22 | 2020-09-18 | Q-莱纳公司 | Sample rack |
| US11673137B2 (en) | 2018-01-22 | 2023-06-13 | Q-Linea Ab | Sample holder |
| CN109030844A (en) * | 2018-07-05 | 2018-12-18 | 领航基因科技（杭州）有限公司 | Automatically generating device and method applied to biosystem microfluid |
| CN109030844B (en) * | 2018-07-05 | 2022-08-05 | 领航基因科技（杭州）有限公司 | Automatic generation device and method applied to biological system microfluid |
| CN108855264A (en) * | 2018-07-12 | 2018-11-23 | 北京乐普智慧医疗科技有限公司 | A kind of multipurpose multi objective micro-fluidic chip |
| CN113134400A (en) * | 2021-05-22 | 2021-07-20 | 杭州霆科生物科技有限公司 | Micro-fluidic chip capable of removing bubbles |
| CN114160223A (en) * | 2021-12-28 | 2022-03-11 | 北京梓晶生物科技有限公司 | Micro-fluidic chip |
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| GR01 | Patent grant | ||
| C41 | Transfer of patent application or patent right or utility model | ||
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Effective date of registration: 20151027 Address after: 100094 Beijing, Haidian District, No. ten on the ground floor, No. 1, building 1014, room 10, room 4 Patentee after: BEIJING BAICARE BIOTECHNOLOGY CO.,LTD. Address before: 116000 No. 59 Xinghai Third Street, Shahekou District, Liaoning, Dalian Patentee before: Zhang Guohao |
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