CN204422311U - A kind of marine bacterioplankton and viral sample fast separation device - Google Patents
A kind of marine bacterioplankton and viral sample fast separation device Download PDFInfo
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- CN204422311U CN204422311U CN201520085932.0U CN201520085932U CN204422311U CN 204422311 U CN204422311 U CN 204422311U CN 201520085932 U CN201520085932 U CN 201520085932U CN 204422311 U CN204422311 U CN 204422311U
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- 238000000926 separation method Methods 0.000 title claims abstract description 12
- 241000721717 unidentified marine bacterioplankton Species 0.000 title 1
- 230000003612 virological effect Effects 0.000 title 1
- 239000007788 liquid Substances 0.000 claims abstract description 51
- 241000700605 Viruses Species 0.000 claims abstract description 40
- 238000002347 injection Methods 0.000 claims abstract description 37
- 239000007924 injection Substances 0.000 claims abstract description 37
- 241000894006 Bacteria Species 0.000 claims abstract description 24
- 238000005070 sampling Methods 0.000 claims abstract description 18
- 238000004458 analytical method Methods 0.000 claims abstract description 5
- 230000001580 bacterial effect Effects 0.000 claims description 19
- 239000012528 membrane Substances 0.000 claims description 9
- 238000001914 filtration Methods 0.000 claims description 7
- 239000011248 coating agent Substances 0.000 claims description 5
- 238000000576 coating method Methods 0.000 claims description 5
- 244000005700 microbiome Species 0.000 claims description 5
- 239000013535 sea water Substances 0.000 abstract description 13
- 230000008014 freezing Effects 0.000 abstract description 3
- 238000007710 freezing Methods 0.000 abstract description 3
- 238000010257 thawing Methods 0.000 abstract description 3
- 241000195493 Cryptophyta Species 0.000 abstract description 2
- 239000013618 particulate matter Substances 0.000 abstract description 2
- 238000012545 processing Methods 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 abstract description 2
- 230000000813 microbial effect Effects 0.000 abstract 1
- 239000011859 microparticle Substances 0.000 description 5
- 239000011148 porous material Substances 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 238000009434 installation Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000000605 extraction Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 241000556533 uncultured marine bacterium Species 0.000 description 1
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Abstract
本实用新型公开一种海洋浮游细菌和病毒样品快速分离装置，包含：一可抽取和注射液体的注射管；一细菌过滤器；一集液管，其两端各设有一开口；一病毒过滤器；一进样管，用于直接置于测量仪器中进行分析测定；各组成部件之间可选择三种不同的拆卸式组合连接，以获得不同的测试样品，并结合流式细胞仪来实现样品中含有微生物的计数。本实用新型结构简单，便于携带，可于调查船使用，满足样品在采集的同时进行分离保存的目的，可以有效避免海水中藻类、浮游生物和颗粒物等在样品保存过程中对其的影响，同时可避免样品处理过程中反复冻融的影响。
The utility model discloses a rapid separation device for marine planktonic bacteria and virus samples, which comprises: an injection tube capable of extracting and injecting liquid; a bacteria filter; a liquid collecting tube with openings at both ends; a virus filter ; A sampling tube, used to be directly placed in the measuring instrument for analysis and determination; three different detachable combined connections can be selected between the components to obtain different test samples, and combined with the flow cytometer to realize the sample Contains microbial counts. The utility model is simple in structure, easy to carry, can be used in survey ships, meets the purpose of separating and storing samples while collecting, and can effectively avoid the influence of algae, plankton and particulate matter in seawater during sample storage, and at the same time The influence of repeated freezing and thawing during sample processing can be avoided.
Description
技术领域 technical field
本实用新型涉及一种海洋浮游细菌和病毒样品分离装置。 The utility model relates to a separation device for marine planktonic bacteria and virus samples.
背景技术 Background technique
现有技术中，使用流式细胞仪对海水样品中的浮游细菌和病毒进行检测和统计。流式细胞仪将海水样品中浮游细菌和病毒进行荧光染色，并通过收集荧光信号对细菌和病毒进行计数。细菌和病毒由于直径不同可获得不同的荧光信号。由于海水中还有一些微型颗粒物有自发荧光，可能对结果存在一定的干扰。另外，目前也无法立刻实现海洋浮游细菌和病毒样品的分离保存；反复冻融可能对实验结果造成影响；人为操作带来可能的污染。 In the prior art, a flow cytometer is used to detect and count planktonic bacteria and viruses in seawater samples. The flow cytometer performs fluorescent staining on planktonic bacteria and viruses in seawater samples, and counts the bacteria and viruses by collecting fluorescent signals. Bacteria and viruses can obtain different fluorescent signals due to different diameters. Since there are still some micro-particles in seawater that have autofluorescence, there may be some interference to the results. In addition, the separation and storage of marine bacterium and virus samples cannot be realized immediately; repeated freezing and thawing may affect the experimental results; human manipulation may cause possible pollution.
发明内容 Contents of the invention
本实用新型的目的在于提供一种海洋浮游细菌和病毒样品快速分离装置，实现海水样品中浮游细菌和病毒样品的分离并即时保存，从而将样品过滤过程中可能造成的人为污染降到最低。 The purpose of the utility model is to provide a rapid separation device for marine planktonic bacteria and virus samples to realize the separation and immediate storage of planktonic bacteria and virus samples in seawater samples, thereby minimizing the possible artificial pollution during the sample filtration process.
解决上述技术问题，本实用新型采用的技术方案如下：一种海洋浮游细菌和病毒样品快速分离装置，包含： To solve the above-mentioned technical problems, the technical scheme adopted by the utility model is as follows: a rapid separation device for marine planktonic bacteria and virus samples, comprising:
一可抽取和注射液体的注射管； a syringe for withdrawing and injecting liquid;
一细菌过滤器； a bacterial filter;
一集液管，其两端各设有一开口； a collecting pipe with an opening at each end;
一病毒过滤器； a virus filter;
一进样管，用于直接置于测量仪器中进行分析测定； A sampling tube, which is used to be directly placed in the measuring instrument for analysis and determination;
上述各组成部件之间为拆卸式组合连接。 The above-mentioned components are connected in a detachable combination.
可选地，所述组合连接为所述注射管的注射口与所述细菌过滤器的入液口相连，所述细菌过滤器的出液口与所述集液管的一开口相连，所述集液管的另一开口连接所述病毒过滤器的入液口，所述病毒过滤器的出液口与所述的进样管的开口相连。 Optionally, the combined connection is that the injection port of the injection tube is connected with the liquid inlet of the bacterial filter, the liquid outlet of the bacterial filter is connected with an opening of the liquid collection pipe, and the The other opening of the collecting pipe is connected with the liquid inlet of the virus filter, and the liquid outlet of the virus filter is connected with the opening of the sampling tube.
可选地，所述组合连接为所述注射管的注射口与所述的细菌过滤器的入液口相连，所述细菌过滤器的出液口与所述集液管的一开口相连，所述集液管的另一开口与所述的进样管的开口相连。 Optionally, the combined connection is that the injection port of the injection pipe is connected with the liquid inlet of the bacterial filter, and the liquid outlet of the bacterial filter is connected with an opening of the liquid collection pipe, so The other opening of the collecting pipe is connected with the opening of the sampling pipe.
可选地，所述组合连接为所述注射管的注射口与所述的进样管的开口相连。 Optionally, the combined connection is that the injection port of the injection tube is connected with the opening of the injection tube.
上述所有的连接均为液密性连接。 All of the above connections are liquid-tight connections.
优选地，所述注射管为无菌一次性注射管。 Preferably, the syringe is a sterile disposable syringe.
优选地，所述的细菌过滤器中设有用于过滤0.2μm大小以上微生物的滤膜。 Preferably, the bacterial filter is provided with a filter membrane for filtering microorganisms with a size above 0.2 μm.
优选地，所述的集液管的管身上设有刻度涂层。 Preferably, a scale coating is provided on the body of the liquid collecting pipe.
优选地，所述的病毒过滤器中设有用于过滤0.02μm以上微生物的滤膜。 Preferably, the virus filter is provided with a filter membrane for filtering microorganisms above 0.02 μm.
优选地，所述的进样管的管身上设有刻度涂层。 Preferably, a scale coating is provided on the body of the sampling tube.
本实用新型结构简单，便于携带，可于调查船使用，满足样品在采集的同时进行分离保存的目的，可以有效避免海水中藻类、浮游生物和颗粒物等在样品保存过程中对其的影响，同时可避免样品处理过程中反复冻融的影响。 The utility model is simple in structure, easy to carry, can be used in survey ships, meets the purpose of separating and storing samples at the same time of collection, and can effectively avoid the influence of algae, plankton and particulate matter in seawater during sample storage, and at the same time The influence of repeated freezing and thawing during sample processing can be avoided.
附图说明 Description of drawings
下面结合附图和具体实施例对本发明作进一步的详细说明： Below in conjunction with accompanying drawing and specific embodiment the present invention is described in further detail:
图1为本实用新型的连接关系之一的组合安装示意图； Fig. 1 is the combined installation schematic diagram of one of connection relation of the present utility model;
图2为本实用新型的连接关系之二的组合安装示意图； Fig. 2 is the combined installation schematic diagram of connection relation 2 of the utility model;
图3为本实用新型的连接关系之三的组合安装示意图。 Fig. 3 is a combined installation schematic diagram of the third connection relationship of the present invention.
附图标记：1-可抽取和注射液体的注射管，2-细菌过滤器，3-集液管，4-病毒过滤器，5-进样管。 Reference numerals: 1—injection tube for extracting and injecting liquid, 2—bacteria filter, 3—liquid collection pipe, 4—virus filter, 5—injection tube.
具体实施方式 Detailed ways
如图1、2、3所示，本实用新型海洋浮游细菌和病毒样品快速分离装置，包含一可抽取和注射液体的注射管1；一细菌过滤器2；一集液管3，其两端各设有一开口；一病毒过滤器4；一进样管5，用于直接置于测量仪器中进行分析测定；各组成部件之间可选择以下三种拆卸式组合连接，以获得不同的测试样品。 As shown in Figures 1, 2, and 3, the utility model marine planktonic bacteria and virus sample rapid separation device includes an injection tube 1 that can extract and inject liquid; a bacterial filter 2; a liquid collection pipe 3, and its two ends Each is provided with an opening; a virus filter 4; a sampling tube 5, which is used to be directly placed in the measuring instrument for analysis and determination; the following three detachable combined connections can be selected between the components to obtain different test samples .
如图1，可将用于抽取和注射液体的注射管1的注射口与细菌过滤器2的入液口相连，细菌过滤器2的出液口与集液管3的一开口相连，集液管3的另一开口连接病毒过滤器4的入液口，病毒过滤器4的出液口与进样管5的开口相连；上述连接关系，在进样管中获得经两次过滤后样品。 As shown in Figure 1, the injection port of the injection tube 1 for extracting and injecting liquid can be connected with the liquid inlet of the bacterial filter 2, and the liquid outlet of the bacterial filter 2 is connected with an opening of the liquid collection pipe 3, and the liquid collection The other opening of the tube 3 is connected to the liquid inlet of the virus filter 4, and the liquid outlet of the virus filter 4 is connected to the opening of the sampling tube 5; the above-mentioned connection relationship obtains the sample filtered twice in the sampling tube.
如图2，可将用于抽取和注射液体的注射管1的注射口与细菌过滤器2的入液口相连，细菌过滤器2的出液口与集液管3的一开口相连，集液管3的另一开口与进样管5的开口相连；上述连接关系，在进样管中获得经一次过滤后样品。 As shown in Figure 2, the injection port of the injection tube 1 for extracting and injecting liquid can be connected with the liquid inlet of the bacterial filter 2, and the liquid outlet of the bacterial filter 2 is connected with an opening of the liquid collection pipe 3, and the liquid collection The other opening of the tube 3 is connected to the opening of the sampling tube 5; in the above connection relationship, the sample after primary filtration is obtained in the sampling tube.
如图3，可将用于抽取和注射液体的注射管1的注射口直接与进样管5的开口相连；在进样管中获得未经过滤的样品。 As shown in Fig. 3, the injection port of the injection tube 1 for extracting and injecting liquid can be directly connected with the opening of the injection tube 5; an unfiltered sample is obtained in the injection tube.
上述所有的连接均为液密性连接，所有的部件均为无菌部件。注射管1可选用无菌一次性注射器，方便样液的抽取。为确保过滤的效果，细菌过滤器2中的滤膜孔径为0.2μm；病毒过滤器4中的滤膜孔径为0.02μm。集液管3和进样管5的管身上都涂有刻度涂层，以方便掌握抽取和推射过程中样液的多少。 All connections mentioned above are liquid-tight connections, and all parts are sterile parts. The injection tube 1 can be a sterile disposable syringe to facilitate the extraction of the sample liquid. In order to ensure the filtering effect, the pore size of the filter membrane in the bacterial filter 2 is 0.2 μm; the pore size of the filter membrane in the virus filter 4 is 0.02 μm. The pipe bodies of the liquid collecting pipe 3 and the sampling pipe 5 are all coated with a scale coating, so as to facilitate the grasp of the amount of the sample liquid during the extraction and injection process.
在实际测量过程中，可以结合流式细胞仪来实现样品中含有微生物的计数，由于海水中还含有一些自发荧光的微型颗粒物（其直径低于浮游病毒），会对结果存在一定的干扰。因此，同一海水样品需分三部分进行测定。 In the actual measurement process, flow cytometry can be combined to realize the count of microorganisms contained in the sample. Since seawater also contains some autofluorescent micro-particles (the diameter of which is smaller than that of planktonic viruses), there will be some interference with the results. Therefore, the same seawater sample needs to be measured in three parts.
首先用一次性注射管1抽取欲检测的海水（体积为V），然后将进样管5的开口直接连接至注射管1的注射口，并将注射管1中一定体积V’（V’≤1/3V）的海水注入进样管5中，取得第一部分样本； First use the disposable injection tube 1 to extract the seawater to be tested (volume V), then directly connect the opening of the injection tube 5 to the injection port of the injection tube 1, and inject a certain volume V' in the injection tube 1 (V'≤ 1/3V) of seawater is injected into the sampling tube 5 to obtain the first part of the sample;
然后，将一细菌过滤器2的入液口直接连接至注射管1的注射口，细菌过滤器2的出液口连接集液管3的上开口，集液管3的下开口连接进样管5，并注射一定体积V’的海水，取得第二部分样本； Then, the liquid inlet of a bacterial filter 2 is directly connected to the injection port of the injection tube 1, the liquid outlet of the bacterial filter 2 is connected to the upper opening of the liquid collection pipe 3, and the lower opening of the liquid collection pipe 3 is connected to the sampling tube 5, and inject a certain volume V' of seawater to obtain the second part of the sample;
最后，在集液管3的下开口连接一病毒过滤器4的入液口，病毒过滤器4的出液口与进样管5相连，随后注射一定体积V’的海水，取得第三部分样本。 Finally, the lower opening of the liquid collection pipe 3 is connected to the liquid inlet of a virus filter 4, and the liquid outlet of the virus filter 4 is connected to the sampling tube 5, and then a certain volume V' of seawater is injected to obtain the third part of the sample .
第一部分样本不经滤膜过滤，样品中含有浮游细菌+浮游病毒+微型颗粒物； The first part of the sample is not filtered by the filter membrane, and the sample contains planktonic bacteria + planktonic virus + micro particles;
第二部分样本经细菌过滤器2中的0.2um孔径滤膜过滤，样品中含浮游病毒+微型颗粒物； The second part of the sample is filtered through the 0.2um pore size filter membrane in the bacterial filter 2, and the sample contains planktonic virus + micro particles;
第三部分样本经病毒过滤器4中的0.02um孔径滤膜过滤，样品中仅含微型颗粒物； The third part of the sample is filtered through the 0.02um pore size filter membrane in the virus filter 4, and only micro particles are contained in the sample;
将第一部分样本、第二部分样本和第三部分样本置入流式细胞仪进行分析，分别得到第一部分样本的计数结果C’、第二部分样本的计数结果B’、第三部分样本的计数结果A’。根据上述流式细胞仪计数结果，就可得出检测海水中含有浮游病毒数量为B’-A’；含有浮游细菌数量为C’-B’。 Put the first part of the sample, the second part of the sample and the third part of the sample into the flow cytometer for analysis, and obtain the counting result C' of the first part of the sample, the counting result of the second part of the sample B', and the counting result of the third part of the sample Result A'. According to the above flow cytometer counting results, it can be concluded that the number of planktonic viruses contained in the detected seawater is B'-A'; the number of planktonic bacteria contained is C'-B'.
本实用新型的实施方式不限于此，根据本实用新型的上述内容，按照本领域的普通技术知识和惯用手段，在不脱离本实用新型基本技术思想前提下，对本实用新型上述结构做出的其它多种形式的修改、替换或变更，均落在本实用新型的保护范围之内。 The implementation of the utility model is not limited thereto. According to the above content of the utility model, according to the common technical knowledge and conventional means in this field, without departing from the basic technical idea of the utility model, other modifications made to the above structure of the utility model Various forms of modification, replacement or change all fall within the protection scope of the present utility model.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109260954A (en) * | 2018-11-30 | 2019-01-25 | 张军毅 | A kind of pressure type sample extraction device |
| CN114980995A (en) * | 2019-12-10 | 2022-08-30 | 阿奎盖德斯技术有限公司 | Two-stage filter for removing microorganisms from water |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109260954A (en) * | 2018-11-30 | 2019-01-25 | 张军毅 | A kind of pressure type sample extraction device |
| CN114980995A (en) * | 2019-12-10 | 2022-08-30 | 阿奎盖德斯技术有限公司 | Two-stage filter for removing microorganisms from water |
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