CN1559194A - Method for breeding sprout by tissue culture, and vessels therefor - Google Patents
Method for breeding sprout by tissue culture, and vessels therefor Download PDFInfo
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- CN1559194A CN1559194A CNA2004100155121A CN200410015512A CN1559194A CN 1559194 A CN1559194 A CN 1559194A CN A2004100155121 A CNA2004100155121 A CN A2004100155121A CN 200410015512 A CN200410015512 A CN 200410015512A CN 1559194 A CN1559194 A CN 1559194A
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- seedling
- cultivating tray
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- seed cultivation
- tissue breeding
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Abstract
A method for breeding the sprout by tissue culture includes such steps as adding nutritive liquid and rooting promoter to light porous matrix to obtain culture medum, loading in seedling culture tray and container, dividing the germinal seedling, inoculating in said matrix of culture tray, loading in culture box, culture in room for rooting, and culturing in greenhouse. Its container has bottom, seedling culture trays and top cover.
Description
Technical field
The present invention relates to a kind ofly carry out the method for seed cultivation by plant tissue culture technique, specifically be improve grow seedlings in the method and the culture vessel thereof in strong sprout and culture of rootage stage.
Background technology
At present, many plants can carry out the cultivation of seedling by Plant Tissue Breeding, and are applied in agricultural production, have formed the scale batch production and have produced, and have promoted the commercialization production of many plants, have produced bigger economic benefit.The method of general tissue culture is in the sealed, sterile vial, with certain culture base and growth regulatory substance, induces the regeneration of plant and breeds a large amount of bud seedlings, and root induction becomes complete seedling on root media then, transplants outer cultivation of bottle and forms.Because high humidity in the air-tight bottle, artificial environments such as stuffiness, growth conditions and land for growing field crops have than big-difference, seedling children is tender, and the allelotaxis is relatively poor, a little less than the ability that conforms during transplanting, the vulnerable to pollution influence, during bottle outlet, it is serious to slander root, leaf and seedling body in the process of cleaning medium, causes transplanting survival rate to reduce.Time-consuming water wasting electricity in the general cultural method, the production cost height becomes limiting factor important in the batch production production application.Pay attention to the foundation of regenerating system in the past Study on tissue culture, less to the technology force method research that improves culture efficiency and seedling quality, along with the development of planting seedling industrialized production, improve tissue culture technique and improve Research on Process and come into one's own, invent sugar-free culture technique, locking type seedling production technology as Japanese scholar, in tissue culture procedures, manually enriched the automation that carbonic acid gas promotes seedling early growth and realizes producing.
Summary of the invention
The object of the invention is to propose the method for a kind of improved strong sprout and culture of rootage and supporting container thereof, and purpose is: (1) cultivates and two the link organic linkings of nursing young plants in hothouses indoor seedling, simplifies production technology; (2) improve seedling to transplanting the adaptive capacity of environment, the damage of seedling to seedling and its root system washed in minimizing; (3) reduce production costs; (4) provide the practical culture vessel that can be suitable for mutually with existing production technology.
Purpose of the present invention realizes by following steps:
(1) preparation medium
Adopt light porous property matrix, add and cultivate nutrient solution and root-inducing promoter, pack into seedling-cultivating tray and cultivation box; Medium places 1.0-1.5kg/cm in the high-pressure sterilizing pot
2Down sterilization 20-30 minute of pressure.
(2) indoor seedling is cultivated
On superclean bench, get bud seedling by tissue culture propagation, be inoculated in the seedling-cultivating tray matrix, in the culture vessel of packing into, in culturing room's culturing rack or seedling cultivation greenhouse, cultivated 15-50 days;
(3) nurse young plants in hothouses
Behind the bud seedling rooting, seedling-cultivating tray takes out from culture vessel, continues in the greenhouse to move into routine after hardening 10-15 days and nurse young plants in hothouses and cultivate in dish or the Seedling bag.
Above-mentioned matrix is with peat, perlite, wood chip or be mixed and form, and orchid is cultivated available sphagna.
During above-mentioned matrix can partially recycledly be utilized again or bring into and be nursed young plants in hothouses.
Above-mentioned steps (3) also can adopt following step to carry out: behind the bud seedling rooting, seedling-cultivating tray takes out from culture vessel, with seedling carrying substrates or the carrying substrates direct transplanting routine hardening and growing seedlings in the dish of nursing young plants in hothouses not.
A kind ofly be used to realize the above-mentioned container that the method for seed cultivation is carried out in Plant Tissue Breeding that passes through, comprise chassis 1, seedling-cultivating tray and loam cake 7, be characterized in that chassis 1 is provided with inside and outside edge, seedling-cultivating tray places in the culture vessel, be made up of little cave seedling-cultivating tray 2 and hollow seedling-cultivating tray 3,7 liang of middle side parts of described loam cake are provided with adjustable bacteriological filtration pore 4.
The function of replenishing moisture content and nutrition can be worked in above-mentioned chassis 1, and its interior edge is high, the outer is low, and interior edge can be used for the support seedling-cultivating tray.
Be groove between the interior outer on above-mentioned chassis 1, built-in O-ring seal.
Above-mentioned little cave seedling-cultivating tray 2 has foveola 10, and little root groove of leading is arranged in the cave.
Above-mentioned adjustable bacteriological filtration pore 4 is positioned at the top of prominent button 6, and symmetrically, can make and cultivate gas flow exchange in the box.Pore 4 is cylindric, on little ventilation hole 9 is arranged, external filter membrane and rotatable covers 8 regulated the ventilation hole sizes, rotatable covers 8 has ventilation hole corresponding with little ventilation hole 9, can make little ventilation hole 9 closed or unlatchings.
Container can be expanded into large-scale culture vessel to scale.The small containers ratio can be supporting with ready-made homemade high-pressure sterilizing pot, raise seedling in greenhouse dish.
Compared with prior art, the present invention has following advantage:
1, indoor root induction cultivation and greenhouse hardening are become a production link and process, simplify production process and operation, shorten incubation time, save cost of labor and reach 20%.
Do not need to wash seedling when 2, bottle outlet is transplanted, reduce damage, improve transplanting survival rate, the about 0.04 yuan/strain of using water wisely cost and recruitment cost seedling.
3, the seedling of the method cultivation, owing to be to grow in the open environment of can ventilating, adaptive capacity is strong, the transplanted seedling survival rate can be stabilized in more than 98%, no seedling-slowing stage, the quality of raising seedling.
4, save the about 0.01 yuan/strain of sucrose use cost in conventional the cultivation.
5, reduce the used agar matrix of culture of rootage, about 0.02 yuan/strain saves production cost.
6, reduce pollution loss.
Description of drawings
Fig. 1 is the STRUCTURE DECOMPOSITION figure that is used to realize being undertaken by Plant Tissue Breeding the method container of seed cultivation;
Fig. 2 is the structural representation of this container;
Fig. 3 is the partial sectional view of this container;
Fig. 4 is the vertical view on chassis;
Fig. 5 is the structural representation of little cave seedling-cultivating tray;
Fig. 6 is the enlarged drawing of A.
Among the figure: chassis 1, little cave seedling-cultivating tray 2, hollow seedling-cultivating tray 3, pore 4, button hole 5, prominent button 6, loam cake 7, rotatable covers 8, little ventilation hole 9, foveola 10.
Embodiment
Embodiment 1:
Cultivating with the flameray gerbera seedling is example:
(1) the preparation culture matrix is made matrix with peat, adds medium composition and the 1mg/L NAA of 0.5 liter of 1/2MS (macroelement reduces by half) in every liter of matrix, mixes thoroughly and packs seedling-cultivating tray into and cultivate box, 1kg/cm in high-pressure sterilizing pot
2Down sterilization 20 minutes of pressure;
(2) on superclean bench, get the above flameray gerbera bud seedling of 2cm of enrichment culture, cut the radical bud seedling, be inoculated in the seedling-cultivating tray matrix, in the culture vessel of packing into, in culturing room's culturing rack or seedling cultivation greenhouse, cultivated 20 days strengthening seedling and rooting.
(3) take root after, can from culture vessel, take out seedling-cultivating tray, continue in the greenhouse hardening and move into routine after 10 days and nurse young plants in hothouses and cultivate in dish or the Seedling bag, cultivate 1-2 and can sell or be used for after the month to produce.
For realizing above method, the invention provides a cover culture vessel.
The design example of supporting culture vessel:
The culture vessel material polypropylene or the Merlon of acid and alkali-resistance and HTHP.
Shown in accompanying drawing 1,2, culture vessel is made up of chassis 1, seedling-cultivating tray and loam cake 7.Chassis 1 is provided with interior outer as shown in Figure 4, and interior edge is high, the outer is low, is groove between interior outer, built-in O-ring seal, and the corner arc, the elasticity button hole is established in 1 both sides, chassis, the double as handle; Loam cake 7 as shown in Figure 6, it is to make with high light transmissive material, rectangle or trapezoidal, both sides are provided with the prominent button that matches with the chassis button hole, and the middle part is provided with adjustable bacteriological filtration pore 4, and pore 4 is cylindric, on little ventilation hole is arranged, external filter membrane and rotatable covers have ventilation hole corresponding with little ventilation hole 9 on the rotatable covers 8, can make little ventilation hole 9 closed or open; Seedling-cultivating tray places in the culture vessel, is divided into little cave seedling-cultivating tray 2 and hollow seedling-cultivating tray 3, and as shown in Figure 3, the seedling dish can be adjusted size; The foveola 10 that tool is used to grow seedlings on the seedling dish 2 of little cave, suitable for reading big, following young, 4 pairs 8 little root grooves of leading are established in the inboard, hole, and as shown in Figure 5, the big I in hole is adjusted according to the kind situation of cultivating seedling, disposes the seedling dish of different holes number.
Embodiment 2:
Cultivating with red palm seedling is example:
(1) the preparation culture matrix is made matrix with wood chip, adds 0.5 liter of 1/2MS medium component and 0.5mg/L NAA in every liter of matrix, mixes pack into seedling-cultivating tray and culture vessel thoroughly, 1.5kg/cm in high-pressure sterilizing pot
2Down sterilization 25 minutes of pressure.
(2) on superclean bench, get the high red palm bud seedling of 2cm of enrichment culture, cut the bud seedling, be inoculated in the seedling-cultivating tray matrix, in the culture vessel of packing into, in culturing room's culturing rack or seedling cultivation greenhouse, cultivated 30 days strengthening seedling and rooting.
(3) take root after, can from cultivate box, take out seedling-cultivating tray, continue in the greenhouse hardening and move into routine after 15 days and nurse young plants in hothouses and cultivate in dish or the Seedling bag, can sell or be used for production after cultivating February.
Embodiment 3:
Cultivating with the The Incredible Hulk seedling is example:
(1) preparation culture matrix with peat, perlite mixed substrate, adds 0.5 liter of 1/2MS medium component and 1mg/L NAA in every liter of matrix, mix pack into seedling-cultivating tray and culture vessel thoroughly, 1.2kg/cm in high-pressure sterilizing pot
2Down sterilization 30 minutes of pressure.
(2) on superclean bench, get the high The Incredible Hulk bud seedling of 2cm of enrichment culture, or the seedling clump, be inoculated in the seedling-cultivating tray matrix, in the culture vessel of packing into, in culturing room's culturing rack or seedling cultivation greenhouse, cultivated 20 days strengthening seedling and rooting.
(3) take root after, can from culture vessel, take out seedling-cultivating tray, get the carrying substrates transplantation of seedlings and go into routine and nurse young plants in hothouses and cultivate in dish or the Seedling bag, cultivate 1-2 and can sell or be used for after the month to produce.
Embodiment 4:
Cultivating with the Moth orchid seedling is example:
(1) the preparation culture matrix is made matrix with sphagna, and sphagna restrains in the peptone prepared culture and soaks in every liter of MS medium component, 20 gram bananas juices, 20 gram apple juices, 1 that contain 1/3 macroelement, mixes in the bottle of packing into 1.2kg/cm in high-pressure sterilizing pot thoroughly
2Down sterilization 30 minutes of pressure, culture vessel is sterilized simultaneously.
(2) on superclean bench, get the above Moth orchid seedling of 2 leaves of tool of aseptic seeding or mitogenetic cultivation, be inoculated in the seedling-cultivating tray, pack into after the adding matrix in the culture vessel, in culturing room's culturing rack or seedling cultivation greenhouse, cultivated 50 days strengthening seedling and rooting.
(3) take root after, can from cultivate box, take out seedling-cultivating tray, with the seedling band not the carrying substrates direct transplanting routine hardening of nursing young plants in hothouses in the cup cultivate.
Claims (10)
1, a kind ofly carries out the method for seed cultivation, it is characterized in that comprising the following steps: by Plant Tissue Breeding
(1) preparation medium
Adopt light porous property matrix, add and cultivate nutrient solution and root-inducing promoter, pack into seedling-cultivating tray and cultivation box; Medium places 1.0-1.5kg/cm in the high-pressure sterilizing pot
2Down sterilization 20-30 minute of pressure.
(2) indoor seedling is cultivated
On superclean bench, get bud seedling by tissue culture propagation, be inoculated in the seedling-cultivating tray matrix, in the culture vessel of packing into, in culturing room's culturing rack or seedling cultivation greenhouse, cultivated 15-50 days;
(3) nurse young plants in hothouses
Behind the bud seedling rooting, seedling-cultivating tray takes out from culture vessel, continues in the greenhouse to move into routine after hardening 10-15 days and nurse young plants in hothouses and cultivate in dish or the Seedling bag.
2, according to claim 1ly carry out the method for seed cultivation, it is characterized in that described matrix is mixed with peat, perlite, wood chip etc. to form by Plant Tissue Breeding, or sphagna.
3, according to claim 1 and 2ly carry out the method for seed cultivation, it is characterized in that described matrix can partially recycledly be utilized again or bring into to nurse young plants in hothouses by Plant Tissue Breeding.
4, method of carrying out seed cultivation by Plant Tissue Breeding according to claim 1 and 2, it is characterized in that described step (3) also can adopt following step to carry out: behind the bud seedling rooting, seedling-cultivating tray takes out from culture vessel, with seedling carrying substrates or the carrying substrates direct transplanting routine hardening and growing seedlings in the dish of nursing young plants in hothouses not.
5, a kind ofly be used to realize the container that carries out the method for seed cultivation by Plant Tissue Breeding as claimed in claim 1, comprise chassis (1), seedling-cultivating tray and loam cake (7), it is characterized in that described chassis (1) is provided with inside and outside edge, seedling-cultivating tray places in the culture vessel, be made up of little cave seedling-cultivating tray (2) and hollow seedling-cultivating tray (3), described loam cake (7) two middle side parts are provided with adjustable bacteriological filtration pore (4).
6, pass through the container that the method for seed cultivation is carried out in Plant Tissue Breeding according to what claim 5 was stated, it is characterized in that described chassis (1) interior along high, the outer is low.
7, pass through the container that the method for seed cultivation is carried out in Plant Tissue Breeding according to what claim 5 was stated, it is characterized in that being groove, built-in O-ring seal between the interior outer on described chassis (1).
8, according to claim 5ly carry out the container of the method for seed cultivation by Plant Tissue Breeding, it is characterized in that both sides, described chassis (1) establish elasticity button hole (5), the both sides of loam cake (7) are provided with the prominent button (6) that matches with button hole (5).
9, according to claim 6ly carry out the container of the method for seed cultivation, it is characterized in that described little cave seedling-cultivating tray (2) has foveola (10), little root groove of leading is arranged in the cave by Plant Tissue Breeding.
10, the container that carries out the method for seed cultivation by Plant Tissue Breeding according to claim 6, it is characterized in that described adjustable bacteriological filtration pore (4) is positioned at the top of prominent button (6), and symmetrically, pore is cylindric, on little ventilation hole (9) is arranged, external filter membrane and rotatable covers (8).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410015512 CN1241470C (en) | 2004-03-02 | 2004-03-02 | Method for breeding sprout by tissue culture, and vessels therefor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410015512 CN1241470C (en) | 2004-03-02 | 2004-03-02 | Method for breeding sprout by tissue culture, and vessels therefor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1559194A true CN1559194A (en) | 2005-01-05 |
| CN1241470C CN1241470C (en) | 2006-02-15 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200410015512 Expired - Fee Related CN1241470C (en) | 2004-03-02 | 2004-03-02 | Method for breeding sprout by tissue culture, and vessels therefor |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103814767A (en) * | 2014-02-07 | 2014-05-28 | 兰州大学 | Quick culturing device for bryophyte experimental materials |
| CN103975832A (en) * | 2014-01-28 | 2014-08-13 | 华南农业大学 | Out-of-tube rooting method for tissue culture seedlings of silver birch |
| CN107155843A (en) * | 2017-05-16 | 2017-09-15 | 江苏省农业科学院 | A kind of method that the seedling industrialized transplanting of Chinese yam tissue culture and potato seed of improvement are cultivated |
| CN107249307A (en) * | 2015-02-18 | 2017-10-13 | 不二精工株式会社 | Plant cultivation equipment |
| CN110731267A (en) * | 2019-10-24 | 2020-01-31 | 中国林业科学研究院热带林业研究所 | Domestication process of acacia melanoxylon hard rooting clone tissue culture seedlings |
| CN113728920A (en) * | 2021-08-03 | 2021-12-03 | 福建省亚热带植物研究所 | Device for plant detoxification, seedling transplanting and rooting culture |
-
2004
- 2004-03-02 CN CN 200410015512 patent/CN1241470C/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103975832A (en) * | 2014-01-28 | 2014-08-13 | 华南农业大学 | Out-of-tube rooting method for tissue culture seedlings of silver birch |
| CN103975832B (en) * | 2014-01-28 | 2016-01-13 | 华南农业大学 | A kind of white wing silvery birch plantlet in vitro outside sprout-cultivating-bottle method |
| CN103814767A (en) * | 2014-02-07 | 2014-05-28 | 兰州大学 | Quick culturing device for bryophyte experimental materials |
| CN107249307A (en) * | 2015-02-18 | 2017-10-13 | 不二精工株式会社 | Plant cultivation equipment |
| CN107249307B (en) * | 2015-02-18 | 2021-03-19 | 不二精工株式会社 | Plant cultivation equipment |
| US11140834B2 (en) | 2015-02-18 | 2021-10-12 | Fuji Seiko Co., Ltd. | Plant cultivation equipment |
| CN107155843A (en) * | 2017-05-16 | 2017-09-15 | 江苏省农业科学院 | A kind of method that the seedling industrialized transplanting of Chinese yam tissue culture and potato seed of improvement are cultivated |
| CN107155843B (en) * | 2017-05-16 | 2020-05-08 | 江苏省农业科学院 | Improved method for industrially transplanting tissue culture seedlings of Chinese yams and cultivating potato seeds |
| CN110731267A (en) * | 2019-10-24 | 2020-01-31 | 中国林业科学研究院热带林业研究所 | Domestication process of acacia melanoxylon hard rooting clone tissue culture seedlings |
| CN110731267B (en) * | 2019-10-24 | 2021-06-18 | 中国林业科学研究院热带林业研究所 | Domestication process of acacia melanoxylon hard rooting clone tissue culture seedlings |
| CN113728920A (en) * | 2021-08-03 | 2021-12-03 | 福建省亚热带植物研究所 | Device for plant detoxification, seedling transplanting and rooting culture |
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| CN1241470C (en) | 2006-02-15 |
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