CN1316991C - Chinese traditional medicine in use for treating endometritis of cow - Google Patents

Abstract

The present invention relates to a Chinese medicine for animals, particularly to a medicine formed prepared by the combination of natural plant medicines and used for treating cattle endometritis. The medicine of the present invention is composed of red sage root, climbing groundsel herb, safflower, honeysuckle stem, forsythia fruit, etc. The present invention has the functions of stimulating circulation to end stasis, inducing menstruation to relieve menalgia, cooling blood, dispersing swelling, obviously resisting bacteria, relieving inflammation, promoting wound healing and resisting free radicals and lipid peroxidation. The present invention has mild estrogen activity and has strong antibacterial action on staphylococcus aureus, colibacillus, pyocyaneum bacteria, etc. and has the advantages of no antibiotic residue and safe use.

Description

A kind of Chinese medicine that is used for the treatment of cattle endometritis

Technical field

The present invention relates to a kind of Chinese medicine for animals, particularly combine, the medicine of treatment cattle endometritis with natural medicinal plants.

Background technology

Endometritis is the uterus of cause pathogeny imcrobe infection animal, causes that endometrium is inflamed, and performance hyperemia, blood stasis, edema, tissue permeability increase, mucosa injury comes off down to a series of pathological processes of suppurating, simultaneously normal physiological function forfeiture.Cattle endometritis is the important diseases that influences dairy development and economic benefit.At China's sickness rate is 10%～30%.This disease causes milch cow infertile, and the tire spacing increases, and milk yield descends.At present, endometritis is mainly treated with antibiotics and chemosynthesis antibacterials.But this type of medicine of life-time service causes pathogen to produce drug resistance, and therapeutic effect descends, and the residual health that directly threatens the people of milk Chinese medicine.

Summary of the invention

The invention provides a kind of novel combine with natural medicinal plants, the medicine of treatment cattle endometritis, this medicine has inhibitory or killing effect and good antiinflammatory action to the pathogenic microorganism that causes inflammation, the effect that promotes that the rheuminess thing is discharged also should be arranged, do not have Drug resistance, and can be in milk the residual material that is pernicious to people.

Medicine of the present invention is to be combined by Radix Salviae Miltiorrhizae, Herba Senecionis Scandentis and Flos Carthami.Its concrete composition ratio is:

Radix Salviae Miltiorrhizae 40g ± 5g Herba Senecionis Scandentis 20g ± 2g Flos Carthami 5g ± 1g.

Can obviously improve its curative effect if in drug regimen of the present invention, add Fructus Forsythiae and Caulis Lonicerae again.The concrete ratio that is added is:

Fructus Forsythiae 15g ± 2g Caulis Lonicerae 20g ± 2g.

The preparation method of medicine of the present invention is: each medical material is put together use water extraction earlier, again the water extract is concentrated into 200%, add ethanol in concentrated solution, make and contain determining alcohol and reach 65%, precipitation leaves standstill the filtering sediment, filtrate heating recovery ethanol, get the water extract of medical material, in water extract, add cosolvent, under the ultrasonic agitation condition, add an amount of distilled water, filter, add the distilled water standardize solution.

The present invention has blood circulation promoting and blood stasis dispelling, inducing menstruation to relieve menalgia, removing heat from blood detumescence; The effect of significant anti-inflammation, promotion wound healing, free radical resisting and anti peroxidation of lipid is arranged; Relatively mild estrogen activity is arranged, staphylococcus aureus, escherichia coli, bacillus pyocyaneus etc. are had stronger antibacterial action, and have antibiotic-free advantage residual, safe in utilization.

The specific embodiment

The present invention is below in conjunction with relevant embodiment detailed explanation:

Below each embodiment carry out the medicine pharmacological testing and

Embodiment 1

Used medicine components is:

Radix Salviae Miltiorrhizae 40g ± 5g Herba Senecionis Scandentis 20g ± 2g Flos Carthami 5g ± 1g

Process for preparing medicine is:

Medical material is put into water soak into post-heating to 90 ℃ extraction 3 times fully, each 2 hours, merge medicinal liquid, be concentrated into 200% after the filtration, in medicinal liquid, add ethanol, make to contain determining alcohol and reach 65%, precipitation leaves standstill the filtering sediment, filtrate heating recovery ethanol, get the water extract of medical material, in water extract, add cosolvent (soil temperature-80), under the ultrasonic agitation condition, add an amount of distilled water, filter, adding distilled water adjusting total amount again is 100ml.

Said medicine is carried out extracorporeal bacteria inhibitor test, and used strain is streptococcus pyogenes, streptococcus uberis, Streptococcus lactis (Lister) Lohnis 1909.554., staphylococcus aureus, escherichia coli, bacillus pyocyaneus.Every kind of each 3 strain of antibacterial, above strain is from the pathological material of disease of the clinical type endometritis of trouble of cattle farms, ground such as Lanzhou, Qingdao, Tianjin collection, through the strain of isolation identification preservation.Testing used culture medium is common steamed beef soup, M-H agar plate, 3% Sanguis caprae seu ovis M-H agar plate.Test method is the cylinder plate method bacteriostatic test, each bacterial strain that is about to preserve on the blood inclined-plane is inoculated into respectively in the mid-37 ℃ of incubators of common steamed beef soup pipe cultivates 20h, after smear for microscopic examination is pure, different strain is carried out the dilution (do trial test earlier and measure optimum diluting multiple) of different multiples.The bacterium liquid that dilution is good is added on the agar plate with sterilization suction pipe absorption 0.1ml that (plate must be fresh, Ying Suiyong is with doing), and with crooked L type Glass rod bacterium liquid is wipeed off immediately, then with little steel bowl after burning slightly on the alcohol burner, equidistant on agar surface vertically-arranged several, each tested medicinal liquid (do trial test earlier and measure optium concentration) and contrast medicinal liquid with variable concentrations adds in the steel bowl respectively then, thereafter change the plate lid into the potlery tile lid, horizontal is put into 37 ℃ of incubators and was cultivated 24 hours, take out with vernier caliper measurement antibacterial circle diameter (mm), every strain bacterium is repeated above-mentioned test and gets its meansigma methods of result several times for 3-4 time, is the antibacterial result of this medicine to this bacterial strain.Testing used control drug is norfloxacin.The criterion of test is: antibacterial circle diameter is a drug resistance less than 10mm; 10-15mm is a medium sensitivity;＞15mm is extremely sensitive.Result of the test sees Table 1.

Embodiment 2

Used trial drug component is:

Radix Salviae Miltiorrhizae 40g ± 5g Herba Senecionis Scandentis 20g ± 2g Flos Carthami 5g ± 1g.Fructus Forsythiae 15g ± 2g Caulis Lonicerae 20g ± 2g.

The method of medication preparation is with embodiment 1.

Undertaken medicine is carried out extracorporeal bacteria inhibitor test by embodiment 1 described method and criterion, result of the test sees Table 1.

Obviously the fungistatic effect of embodiment 2 obviously is better than embodiment 1, but the fungistatic effect of two embodiment all is worse than norfloxacin.Embodiment 2 is most preferred embodiment by testing as can be known.

Medicine with embodiment 2 is the test of filling agent treatment cow endometritis

Table 1

Antibacterial		Embodiment 2		Embodiment 1		Norfloxacin
								Kind	The bacterial strain number	Drug level (mg/ml)	Antibacterial circle diameter (mm)	Drug level (mg/ml)	Antibacterial circle diameter (mm)	Drug level (mg/ml)	Antibacterial circle diameter (mm)
Streptococcus pyogenes streptococcus uberis Streptococcus lactis (Lister) Lohnis 1909.554. staphylococcus aureus e coli	3 3 3 3 3	20 20 20 20 20	13.2 13.6 13.8 14.7 15.1	40 40 40 40 20	12.5 12.1 11.7 13.3 15.6	1 1 1 1 1	16.8 17.4 18.7 17.6 34.8
								Bacillus pyocyaneus	3	80	13.9	80	12.0	1	23.9

Test material and method

1.1 case

2000～2002 years milch cow 148 examples 1.1.1 originate from cattle farm, Duan Jia beach, Lanzhou and five roads, Fengning, Hebei province county battalion milk raising base selection spontaneous generation endometritis.

1.1.2 kind is mainly Fresian, is Xi Mentaer and the improvement cattle of working as bamboo top on a small quantity.

1.1.3 20～40 days 72 examples of the course of disease, 41～60 days 45 examples, 31 examples more than 61 days are the longest 129 days.

Have or not the rheuminess thing to discharge 1.1.4 1. diagnosis is seen, poultry raiser, poultry master or artificial insemination person main suit see cow and discharge the rheuminess thing, see the rheuminess thing during inspection or discharge after the vestige that stays (oxtail, buttocks portion, to fly knot filthy, have pus crusts), can tentatively determine to suffer from clinical type endometritis; 2. examination per rectum checks that residing position, uterus, the shape of cornua uteri, the size in uterus, quality, contractile response, ovary have or not corpus luteum, further judge the weight of inflammation; 3. carry out examination per vagina by prolapsus appliance, check the first transaction of a day's business degree, the color of cervical orifice and have or not pus to flow out, check the amount and the character of pus, determine the inflammation character and the order of severity.

1.1.5 148 examples that characteristic of disease examination is controlled are clinical type endometritis, purulence endometritis 98 examples wherein, Catarrhal endometritis 50 examples.

1.1.6 be divided into 2 groups, 2 groups of embodiment and mycillin matched group.

1.2 treatment

1.2.1 the pure Chinese medicinal preparation of medicine embodiment 2; Penicillin and streptomycin, commercially available.

1.2.2 method of treatment is uterus perfusion administration for 2 groups.2 groups of each 100ml of embodiment, the next day 1 time, 4 times was 1 course of treatment; The each penicillin of mycillin group 2,400,000 units, streptomycin 1,000,000 units are dissolved in uterus perfusion in the 30ml normal saline, and once a day, 4 times was 1 course of treatment.2 courses of treatment, healing person did not use the other drug treatment instead.

1.23 efficacy determination is divided into healing, improvement and invalid.Cure, clinical symptom disappearance, it is normal that uterine function recovers, and mucus is normal when oestrusing and oestrusing, and can carry out artificial insemination; Take a turn for the better, clinical symptom disappearance, uterine function recovers normally substantially, but mucus also has unusually when oestrusing, and still can not carry out artificial insemination; Invalid, clinical symptoms and uterus function all do not have and are clearly better.

Result of the test sees Table 2

Table 2

Group	Case load	1 course cure rate (%)	2 course cure rates (%)	Take a turn for the better (%)	Total effective rate (%)	Inefficiency (%)	Controlled cattle pregnancy rate (%)
								2 groups of embodiment	88	79.55 (70/88)	92.04 (81/88)	3.41 (3/88)	95.45 (84/88)	4.55 (4/88)	84.91 (74/88)
The mycillin contrast	20	65.00 (13/20)	80.00 (16/20)	10.00 (2/20)	90.00 (18/20)	10.00 (2/20)	75.00 (15/20)

Result of the test shows that the embodiment of the invention 2 medicines show the good therapeutic effect that gets.And the mycillin matched group is treated 20 examples altogether, and 1 course cure rate is 65%, 2 course cure rate 80%, total effective rate 90%, and being controlled the cattle pregnancy rate is 75%, every index all is lower than the present invention.

Endometritis is the cause pathogeny imcrobe infection uterus, causes that endometrium is inflamed, and performance hyperemia, blood stasis, edema, tissue permeability increase, mucosa injury comes off down to a series of pathological processes of suppurating, simultaneously normal physiological function forfeiture.Therefore, the medicine of endometritis at first will have inhibitory or killing effect to the pathogenic microorganism that causes inflammation, and it is less important good antiinflammatory action, and the effect that promotes that the rheuminess thing is discharged also will be arranged.And the present invention is made up of Radix Salviae Miltiorrhizae, Herba Senecionis Scandentis, Flos Carthami, Caulis Lonicerae and Fructus Forsythiae.Radix Salviae Miltiorrhizae, blood circulation promoting and blood stasis dispelling, inducing menstruation to relieve menalgia, removing heat from blood detumescence; The effect of significant anti-inflammation, promotion wound healing, free radical resisting and anti peroxidation of lipid is arranged; Relatively mild estrogen activity is arranged.Herba Senecionis Scandentis, heat-clearing and toxic substances removing, the removing heat from blood detumescence, water decoction has broad-spectrum antibacterial action, and staphylococcus aureus, escherichia coli, bacillus pyocyaneus etc. are had stronger antibacterial action, is usually used in various infectious disease, as furuncle, carbuncle, cellulitis, metritis etc.The Fructus Forsythiae heat-clearing and toxic substances removing, dispersing swelling and dissipating binds, it is the key medicine that Chinese veterinarian treats the carbuncle furuncle, multiple Gram-positive and gram negative bacteria all there is inhibitory action, experimental inflammatory model animal there are tangible antiinflammatory, exudation and reduce the effect of scorching kitchen range vascular fragility, promote the formation of inflammatory barrier, thereby and can strengthen the defense function of the phagocytic activity enhancing body of inflammatory exudation cell.Caulis Lonicerae, heat-clearing and toxic substances removing, dredge the meridian passage contain chlorogenic acid and luteolin isoreactivity composition, and chlorogenic acid has antibacterial action more widely, can increase uterus tension force; Luteolin concentration suppressed staphylococcic growth at 1: 350000 o'clock, and other bacterium is also had inhibitory action, and experimental inflammatory model animal is had tangible antiinflammation, made and oozed out obvious minimizing, also can recover immunologic function.Flos Carthami, promoting blood circulation to restore menstrual flow, stasis-dispelling and pain-killing can excited uterus, and low dose can make the uterus rhythmicity shrink, and heavy dose can make the uterus shrink reinforcement automatically; Tangible antiinflammatory action is arranged, reduce the capillary permeability increase that inflammation causes.Radix Salviae Miltiorrhizae, Herba Senecionis Scandentis, Caulis Lonicerae and Fructus Forsythiae all have bacteriostasis preferably in the side, can directly eliminate the pathogenic microorganism that causes endometritis; Radix Salviae Miltiorrhizae, Caulis Lonicerae, Fructus Forsythiae and Flos Carthami all have antiinflammatory action, can suppress tissue inflammation, reduce inflammatory exudation; The excited uterus effect of Flos Carthami, the increase uterus tension force effect of chlorogenic acid, the relatively mild estrogen activity of Radix Salviae Miltiorrhizae all help the discharge of intrauterine rheuminess thing, help improving the uterus blood circulation, promote the uterus functional recovery.Clinical treatment test shows, the present invention of pure Chinese medicinal preparation is at cure rate, the total effective rate of 2 courses of treatment and controlled and all be better than mycillin aspect the leading indicator such as cattle pregnancy rate, and has residual, the safe characteristics of antibiotic-free.Therefore the present invention is the special agent of a very promising treatment cow endometritis.

Claims (3)

1, a kind of Chinese medicine composition for the treatment of cattle endometritis is characterized in that the active ingredient of compositions is made up of following drug extract:

Radix Salviae Miltiorrhizae 40g ± 5g Herba Senecionis Scandentis 20g ± 2g Flos Carthami 5g ± 1g.

2,, it is characterized in that also having in the active ingredient of compositions the extract of following medicine according to the described Chinese medicine composition of claim 1:

Fructus Forsythiae 15g ± 2g Caulis Lonicerae 20g ± 2g.

3, according to the preparation method of claim 1 or 2 described Chinese medicine compositions, it is characterized in that earlier the medical material water extraction, the gained extracting solution is concentrated into 200%, add ethanol more therein and precipitate, after sediment is removed in filtration, filtrate heating recovery ethanol, get the water extract of medicine, add cosolvent in water extract, add an amount of distilled water under the ultrasonic agitation condition, gained liquid is handled the back more after filtration and is added the distilled water standardize solution.