CN1348104A - Fast test biochip for systemic lupus erythematosus - Google Patents
Fast test biochip for systemic lupus erythematosus Download PDFInfo
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- CN1348104A CN1348104A CN 01126977 CN01126977A CN1348104A CN 1348104 A CN1348104 A CN 1348104A CN 01126977 CN01126977 CN 01126977 CN 01126977 A CN01126977 A CN 01126977A CN 1348104 A CN1348104 A CN 1348104A
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Abstract
The present invention is one fast test biochip for systemic lupus erythematosus. The fast systemic lupus erythematosus diagnosing biochip carrier kit includes carrier box and protein test paper strips and has inside the back plate four or more carrier slots with diagnosing test paper strip for testing antinuclear antibody, anti-dsDNA antibody, anti-Sm antibody, anti-ssDNA antibody and other specific protein antibody. The present invention is used in detection and diagnosis of systemic lupus erythematosus in public health self test and clinical test and diagnosis in hospital.
Description
The present invention relates to a kind of systemic lupus erythematosus diagnosis pick-up unit, particularly relate to a kind of fast test biochip for systemic lupus erythematosus.
Systemic loupus erythematosus (SLE) is a kind of autoimmunity connective tissue disease (CTD), forms vasculitis by antigen and antibody mediated immunity reaction, can cause the tissue damage of different parts, produces corresponding clinical manifestation.In China, 70 patients are arranged among per 100,000 crowds approximately, wherein the women accounts for 90%, and often is the women of child-bearing age, and is very harmful.The SLE clinical manifestation is changeable, and it can invade 1-2 organ in early days, thus the performance be not true to type, fail to pinpoint a disease in diagnosis easily.Then may invade a plurality of organs with stadium development, the patient is alleviated with outbreak and is alternately process, causes clinical manifestation very complicated, for clinical diagnosis with detect and brought very big obstruction.Therefore development and raising are to the diagnostic method of SLE, for finding and diagnosis SLE to have extremely important clinical meaning fast.
The existence of polytype antinuclear antibodies is important serology characteristics of suffering from SLE, so the quick diagnosis of SLE mainly is according to the inspection of serum autoantibody at present, again in conjunction with observing with clinical manifestation.Wherein antinuclear antibodies (ANA) is 95% to the susceptibility of SLE, and specificity is approximately 65%, is best at present SLE state of an illness screening index, and as repeatedly being negative, the possibility of then suffering from SLE is little; Up to 95%, susceptibility is about 70% to anti-dsDNA antibody (dsDNA) to the specificity of SLE, to making a definite diagnosis SLE and judging that the lupus activity has bigger reference value; Up to 99%, but susceptibility only is 25% to anti-Sm antibody, also can be positive when the SLE inertia to the specificity of SLE, so many important bases as retrospective diagnosis; Anti-ssDNA antibody (ss-DNA) also can be used as one of SLE diagnosis index in addition.In the present clinical blood examination to the detection of the above four kinds of index, mainly be to adopt radioimmunoassay (RIA), though it has sensitivity preferably, specificity and stability, but testing process is comparatively loaded down with trivial details, operation inconvenience, and also can only analyze a kind of index at every turn, last longer, the amount of diagnostic information that is provided is limited and not comprehensive, and many deficiencies are that clinical diagnosis and treatment have brought inconvenience.
The objective of the invention is to provide a kind of systemic loupus erythematosus (SLE) fast detecting biochip, by parallel joint-detection antinuclear antibodies (ANA), anti-dsDNA antibody (dsDNA), anti-Sm antibody and anti-ssDNA antibody multiple diagnosis indexs such as (ssDNA), can diagnose SLE quickly and accurately, the SLE state of an illness is made preliminary assessment, improve susceptibility and specificity the SLE diagnosis.
For achieving the above object, the present invention has adopted following technical scheme: a kind of fast test biochip for systemic lupus erythematosus, it is made up of carrier box and protein test strips, described carrier box contains carrier box panel and carrier box backboard, be provided with the carrier channels that to place four above test strips in the described carrier box backboard, described test strips is by the suction zones of upper end, the observation district of middle-end and the sample introduction district of lower end constitute, described panel bottom is an injection port, the middle part is the control line-transect between observation panel and injection port and the observation panel, place respectively in the described carrier channels and be used to detect serum antinuclear antibodies (ANA), anti-dsDNA antibody (dsDNA), the protein test strips of anti-Sm antibody and anti-ssDNA antibody indexs such as (ssDNA), utilize the integrated of chip technology, the principle of high flux and parallelization, these protein test paper are integrated with parallel or fan-shaped mode combination in any, form systemic loupus erythematosus detection of biological chip, described biochip also can addition detect other index such as anti-RNP antibody on the basis of above four kinds of indexs, anti-SSA antibody, anti-SSB antibody, anti erythrocyte antibody, the protein test strips that the protein-specific antibody of antiplatelet antibody etc. is made, multiple diagnosis index in the disposable test sample of its energy.
The present invention has not only improved in the clinical blood examination time-consuming, the deficiency such as operation is loaded down with trivial details, cost an arm and a leg because of adopting that the RIA method caused, and than the abundant information that obtains from single albumen diagnosis test paper, can realize the multiple index of disposable fast detecting, and simple to operate, quick, easy to use, naked eyes or machine-readable result, the accuracy height, can improve the reliability of SLE diagnosis, thereby have very high clinical use value for doctor and patient provide more comprehensively clinical diagnosis information.
Below in conjunction with accompanying drawing the embodiment of the invention is done further statement.
Fig. 1 fast test biochip for systemic lupus erythematosus carrier box base synoptic diagram.
Fig. 2 fast test biochip for systemic lupus erythematosus carrier box synoptic diagram.
Fig. 3 protein test strips synoptic diagram.
With reference to Fig. 1, Fig. 2 and Fig. 3, fast test biochip for systemic lupus erythematosus of the present invention is made up of carrier box (1) and protein test strips (8), described carrier box (1) contains carrier box panel (2) and carrier box backboard (3), described carrier box backboard (3) is fan-shaped, be provided with the carrier channels (4) that to place four protein test strips in the carrier box, described test strips (8) is by the suction zones (11) of upper end, the observation district (10) of middle-end and the sample introduction district (9) of lower end constitute, described panel bottom is injection port (5), the middle part is the control line-transect (7) between observation panel (6) and injection port (5) and the observation panel (6), places respectively in the carrier channels and can detect antinuclear antibodies (ANA), anti-dsDNA antibody (dsDNA), the protein test strips (8) of anti-Sm antibody and anti-ssDNA antibody (ssDNA).
During use, when when injection port (5) adds the serum specimen that will detect, contained each index can combine with the golden labeling antibody in the sample introduction district (9) on the protein test strips of this index of biochip relevant detection in the serum, distinguish (10) along the membrane chromatographic of each test paper to observing respectively, and with detection line on the bag quilt specific antibody combine, in observation panel (6), form macroscopic red stripes, excessive compound or unconjugated gold mark thing can continue chromatography, the macroscopic red lines of formation combine with the antibody of control line (quality control line), article two, red lines then show the positive, if have only the quality control line to form red lines then show feminine gender, if detection line and quality line all not colour developing show invalid.So just can disposable joint-detection go out antinuclear antibodies in the serum sample (ANA), anti-dsDNA antibody (dsDNA), anti-Sm antibody and anti-ssDNA antibody (ssDNA), for the rapid diagnosis system lupus erythematosus provides foundation.
Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.The experimental technique of unreceipted actual conditions among the embodiment can be according to the condition described in normal condition such as the laboratory manual, or the condition of advising according to manufacturer.
Claims (2)
1. fast test biochip for systemic lupus erythematosus, it is made up of carrier box (1) and protein test strips (8), described carrier box (1) contains carrier box panel (2), carrier box backboard (3), the middle part of described carrier box panel (2) is provided with observation panel (6), the bottom is provided with injection port (5), be control line-transect (7) between observation panel and the injection port, the upper end of described protein test strips (8) is suction zones (11), middle-end is for observing district (10), the lower end is sample introduction district (9), it is characterized in that described carrier box backboard is provided with the carrier channels that (comprises four) more than four and deposit the albumen test strips, lays in the described carrier channels and can detect antinuclear antibodies (ANA), anti-dsDNA antibody (dsDNA), the test strips of anti-Sm antibody and anti-ssDNA antibody diagnosis indexs such as (ssDNA).
2. according to the described fast test biochip for systemic lupus erythematosus of claim 1, it is characterized in that in the carrier channels in the described carrier box backboard test strip of can also addition making by other protein specific antibody.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01126977 CN1348104A (en) | 2001-10-09 | 2001-10-09 | Fast test biochip for systemic lupus erythematosus |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 01126977 CN1348104A (en) | 2001-10-09 | 2001-10-09 | Fast test biochip for systemic lupus erythematosus |
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| CN1348104A true CN1348104A (en) | 2002-05-08 |
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| CN 01126977 Pending CN1348104A (en) | 2001-10-09 | 2001-10-09 | Fast test biochip for systemic lupus erythematosus |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104597244A (en) * | 2013-10-30 | 2015-05-06 | 吴庄民 | Method for detecting anti-mt DNA antibody enzyme-linked immunoassay based on epitope antigen peptide and application thereof |
| CN105021811A (en) * | 2015-07-27 | 2015-11-04 | 厦门拜尔杰生物科技有限公司 | Antinuclear antibody combined detection kit and detection method thereof |
| CN106086175A (en) * | 2006-04-24 | 2016-11-09 | 健泰科生物技术公司 | For detecting the method and composition of autoimmune conditions |
| CN111077001A (en) * | 2020-01-12 | 2020-04-28 | 天津市宝坻区人民医院 | Method for preparing anti-platelet antibody biological slice |
| CN116359498A (en) * | 2023-02-23 | 2023-06-30 | 兰州兽研生物科技有限公司 | Immune chromatography test paper card for joint inspection of foot-and-mouth disease, african swine fever, swine fever and porcine reproductive and respiratory syndrome virus antibodies |
-
2001
- 2001-10-09 CN CN 01126977 patent/CN1348104A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106086175A (en) * | 2006-04-24 | 2016-11-09 | 健泰科生物技术公司 | For detecting the method and composition of autoimmune conditions |
| CN106086175B (en) * | 2006-04-24 | 2020-03-03 | 健泰科生物技术公司 | Methods and compositions for detecting autoimmune disorders |
| CN104597244A (en) * | 2013-10-30 | 2015-05-06 | 吴庄民 | Method for detecting anti-mt DNA antibody enzyme-linked immunoassay based on epitope antigen peptide and application thereof |
| CN105021811A (en) * | 2015-07-27 | 2015-11-04 | 厦门拜尔杰生物科技有限公司 | Antinuclear antibody combined detection kit and detection method thereof |
| CN111077001A (en) * | 2020-01-12 | 2020-04-28 | 天津市宝坻区人民医院 | Method for preparing anti-platelet antibody biological slice |
| CN111077001B (en) * | 2020-01-12 | 2022-05-10 | 天津市宝坻区人民医院 | Method for preparing anti-platelet antibody biological slice |
| CN116359498A (en) * | 2023-02-23 | 2023-06-30 | 兰州兽研生物科技有限公司 | Immune chromatography test paper card for joint inspection of foot-and-mouth disease, african swine fever, swine fever and porcine reproductive and respiratory syndrome virus antibodies |
| CN116359498B (en) * | 2023-02-23 | 2024-06-11 | 兰州兽研生物科技有限公司 | Immune chromatography test paper card for joint inspection of foot-and-mouth disease, african swine fever, swine fever and porcine reproductive and respiratory syndrome virus antibodies |
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