CN1200269C - Multiprobe optical fibre evanescent wave biological sensor - Google Patents
Multiprobe optical fibre evanescent wave biological sensor Download PDFInfo
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- CN1200269C CN1200269C CN 03115586 CN03115586A CN1200269C CN 1200269 C CN1200269 C CN 1200269C CN 03115586 CN03115586 CN 03115586 CN 03115586 A CN03115586 A CN 03115586A CN 1200269 C CN1200269 C CN 1200269C
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- 239000013307 optical fiber Substances 0.000 title claims abstract description 114
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- 238000001514 detection method Methods 0.000 claims abstract description 13
- 239000000523 sample Substances 0.000 claims description 77
- KPHWPUGNDIVLNH-UHFFFAOYSA-M diclofenac sodium Chemical compound [Na+].[O-]C(=O)CC1=CC=CC=C1NC1=C(Cl)C=CC=C1Cl KPHWPUGNDIVLNH-UHFFFAOYSA-M 0.000 claims description 18
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Abstract
The multiprobe optical fiber evanescent wave biosensor comprises a laser excitation light path, a fluorescence receiving light path, a sample flow path and a scanning driving system, which are respectively used for exciting the fluorescence of a detected biological substance on the surface of an optical fiber core wire, receiving a fluorescence signal from an optical fiber and completing photoelectric conversion, sucking a sample and scanning and detecting the fluorescence signals on a plurality of optical fibers. The invention has the characteristics of high fluorescence excitation efficiency, high light energy utilization rate, high fluorescence receiving efficiency and high detection efficiency.
Description
Technical field:
The present invention relates to a kind of many probe optical fiber Shu ripple biology sensor that dies, this sensor is used widely in fields such as biomedicine, Food Inspection, environmental monitorings, it can survey hormone, polluter, noxious material, explosive, DNA (deoxyribonucleic acid) (DNA), virus, various bacteriums multiple biological substances such as (as anthrax, Escherichia coli O 157s etc.), the dynamic process of all right monitoring bio reaction.
Background technology:
Many probe optical fiber Shu evanescent wave that produces when the ripple biology sensor adopts light wave to transmit in the total reflection mode in optical fiber that dies excites the biomolecule that is marked with fluorescent dye that is attached to the optical fibre core surface with biological compatible reaction.The optical fiber of the surface having been fixed biological identification molecule places sample, in the sample mark biomolecule of fluorescent dye, if come from a kind of biology with the biological identification molecule that is fixed on the optical fibre core surface, then compatible reaction will take place in both, and the tested biomolecule in the sample is attached to optical fiber surface together with the fluorescent dye of institute's mark.An optical fiber can only be surveyed the attribute and the content thereof of a kind of biological substance in the sample, and multifiber is placed a sample simultaneously, then can survey the attribute and the content thereof of multiple biological substance in this sample simultaneously.
Formerly in the technology, an optical fiber Shu who is published in the 57th～62 page of SPIE the 904th volume by people such as the D.E.Yoshida of Univ Utah USA (University of Utah) die the ripple biology sensor structure as shown in Figure 1.This sensor mainly is made up of laser excitation light path and fluorescence receiving light path.Laser excitation optical routing LASER Light Source 1 wherein, plane mirror 2, dichronic mirror 3, coupled lens 4, optical fiber 5 are formed, and the focus of coupled lens 4 is positioned at the input end face 501 of optical fiber 5, and optical fiber 5 places the sample 601 of sample cell 6; The fluorescence receiving light path mainly is made up of coupled lens 4, dichronic mirror 3, fluorescent optical filter 7, condenser lens 8, photodetector 9 and computing machine 10, the optical axis angle at 45 of the surface of dichronic mirror 3 and fluorescence receiving light path.
The shortcoming of above-mentioned technology formerly is:
1. fluorescence excitation efficient is low.Focus on the laggard optical fiber 5 of going into by the laser beam of LASER Light Source 1 output by coupled lens 4, the laser beam numerical aperture that enters optical fiber 5 is little, and the order of reflection of light beam in optical fiber 5 is few; And the ranges of incidence angles of laser beam that enters optical fiber 5 is since 0 °, because light angle is more little, intensity is big more, and the launching efficiency of the more little light of angle is low more.The order of reflection of low-angle light in optical fiber is few simultaneously, and penetration depth is little, excites scope little.
2. the efficiency of light energy utilization is low.Laser beam enters from optical fiber 5 input end faces 501, penetrates from output end face 503, and this part light is not reused.So the utilization factor of laser beam has only 50%.
3. the fluorescence receiving efficiency is low.The fluorescence signal that is inspired by laser beam is from the two ends outgoing of optical fiber 5, and from the structure of Fig. 1, find out, have only from the fluorescence signal of optical fiber 5 input end faces 501 outgoing and be received, be not collected from the fluorescence signal of output end face 503 outgoing, therefore the receiving efficiency to fluorescence also has only 50% of resultant signal.
Above-mentioned three reasons cause the detection sensitivity of technology formerly lower, have only 1.4 * 10
-8Mol.
4. detection efficiency is low.Have only an optical fiber in the sample cell 6 of this sensor, once can only survey a kind of biological substance in the sample 601.
Summary of the invention:
The technical problem to be solved in the present invention is to overcome the shortcoming of above-mentioned technology formerly, provides a kind of improved many probe optical fiber Shu the ripple biology sensor that dies, to improve its fluorescence excitation efficient, the efficiency of light energy utilization, fluorescence receiving efficiency and detection efficiency.
Technical solution of the present invention is as follows:
A kind of many probe optical fiber Shu ripple biology sensor that dies includes laser excitation light path and fluorescence receiving light path, is characterized in:
1. described laser excitation light path, comprise LASER Light Source, on the light beam working direction of sending along this LASER Light Source, be disposed with first conical mirror, second conical mirror, dichronic mirror, coupled lens and optical fiber, the focus of this coupled lens overlaps with the center of optic fibre input end face;
2. described fluorescence receiving light path from optical fiber, is followed successively by coupled lens, dichronic mirror, fluorescent optical filter, condenser lens, diaphragm and photodetector;
The optical axis OO of 3. described laser excitation light path and the optical axis O of fluorescence receiving light path
1O
1Mutually vertical, and equal surperficial at 45 with dichronic mirror;
4. also have sample flow path, be made up of sample cell, sample inlet, sample export and liquid pump, can arrange multifiber one-dimensionally equally spacedly in the described sample cell, the central point of each optic fibre input end face is positioned on the straight line, promptly on the x axle;
5. also have scan drive system, by computing machine with form with the mobile platform of controlled driving, sample cell places on this mobile platform;
6. the electric signal that described computing machine is used to the to gather photodetector output line data of going forward side by side is handled, and this computing machine is also controlled the work of mobile platform and liquid pump, detects with the scanning of finishing the multifiber fluorescence signal.
The front surface of described first conical mirror is the plane, the rear surface is a concave surface, the front surface of second conical mirror is a convex surface, the rear surface is the plane, the bus of the circular conical surface of two conical mirrors and the angle on plane are θ, the span of this θ is 5 °～45 °, and the span of the distance L between the two conical mirror summits is 5mm～20mm.
Described optical fiber is many root multimode fibers, and a part of covering of every optical fiber is removed, and exposes heart yearn, and the surface of every heart yearn is fixed with different biological identification molecules.
Described fiber-optic output face is coated with the broadband reflection dielectric film.
Described diaphragm is positioned at the conjugate plane place of optic fibre input end.
The present invention compares with technology formerly has following technique effect:
1. fluorescence excitation efficient height.On the one hand, circular Gaussian beam is coupled the input end face of lens focus in optical fiber after becoming annular beam by first conical mirror and second conical mirror again, thereby increased the incident angle of laser beam in optical fiber, the order of reflection of laser beam in optical fiber increased, can inspire more fluorescent energy; On the other hand, the incident angle of light is big more in optical fiber, and its evanescent wave penetration depth is big more, to the optical fiber surface fluorescent dye excite scope big more, this can inspire more fluorescent energy equally.
2. efficiency of light energy utilization height.The output end face of optical fiber is coated with broadband medium total reflection film, and the excitation laser beam that incides this end face is reflected back in the optical fiber, and twice fluorescence excitation makes the efficiency of light energy utilization improve 1 times.
3. fluorescence receiving efficiency height.The output end face that is coated with broadband medium total reflection film of optical fiber not only can reflect excitation laser beam, and can reflect back into optical fiber to the fluorescence that is transferred to the fiber-optic output face, after Optical Fiber Transmission, enter the fluorescence receiving light path again, make the fluorescence receiving efficiency also improve 1 times.So total fluorescence signal intensity of the present invention is 4 times of technology formerly.
4. detection efficiency height.Placed multifiber in the sample cell of the present invention simultaneously, different biological identification molecules has been fixed on different optical fibre core surfaces, so multiple biological substance in the once detectable sample.And formerly have only an optical fiber in the sample cell 6 of technology, once can only survey a kind of biological substance in the sample.If placed the optical fiber that different biological identification molecules have been fixed on many surfaces in the sample cell 6 of the present invention, detection efficiency then of the present invention is many times of technology formerly.
Description of drawings:
Fig. 1 is the die structural representation of ripple biology sensor of technology optical fiber Shu formerly.
Fig. 2 is the present invention's die structural representation of ripple biology sensor of optical fiber Shu of popping one's head in more.
Fig. 3 is the present invention's optical fiber Shu beam converter light path synoptic diagram that first conical mirror 11 in the ripple biology sensor and second conical mirror 12 form that dies of popping one's head in more.
Fig. 4 is the present invention's the die cross sectional representation of first conical mirror, the 11 circular Gaussian beam AA of plane of incidence place and second conical mirror, the 11 annular beam BB of exit facet place in the ripple biology sensor of optical fiber Shu of popping one's head in more.Wherein Fig. 4-1 is the cross sectional representation of first conical mirror, the 11 circular Gaussian beam AA of plane of incidence place, and Fig. 4-2 is cross sectional representation of second conical mirror, the 12 annular beam BB of exit facet place.
Embodiment:
See also Fig. 2 earlier, Fig. 2 is the present invention's die structural representation of ripple biology sensor of optical fiber Shu of popping one's head in more, as seen from the figure, the present invention's optical fiber Shu ripple biology sensor that dies of popping one's head in comprises laser excitation light path, fluorescence receiving light path, sample flow path and scan drive system more, be respectively applied for the tested biological substance on excitation fiber heart yearn 502 surfaces fluorescence, receive from the fluorescence signal of optical fiber 5 and finish the suction of opto-electronic conversion, sample and the scanning of fluorescence signal on the multifiber is detected.
On the laser excitation light path, on the circular Gaussian beam AA working direction of sending along LASER Light Source 1, be equipped with first conical mirror 11, second conical mirror 12, dichronic mirror 3, coupled lens 4, optical fiber 5 successively, the focus of coupled lens 4 overlaps with the center of optical fiber 5 input end faces 501.On the fluorescence receiving light path,, be equipped with coupled lens 4, dichronic mirror 3, fluorescent optical filter 7, condenser lens 8, diaphragm 13, photodetector 9 successively from optical fiber 5.The optical axis OO of laser excitation light path and the optical axis O of fluorescence receiving light path
1O
1Mutually vertical, and all with the angle surperficial at 45 of dichronic mirror 3.Sample flow path is made up of sample cell 6, sample inlet 602, liquid pump 14 and sample export 603.Place multifiber in the sample cell 6, the multifiber one dimension is equidistantly arranged, and the central point of each optic fibre input end face is positioned on the straight line.Biomolecular labeling in the sample 601 in the sample cell 6 fluorescent dye.The optical fiber 5 that contacts with sample 601 in the sample cell 6 is to remove covering and the surperficial optical fibre core 502 that is equipped with biological identification molecule, fiber-optic output face 503 plating ATR Optical films.Scan drive system is made up of computing machine 10 and mobile platform 15.Sample cell 6 places above the mobile platform 15, moves as one-dimensional scanning along the orientation of multifiber 5 with mobile platform 15.Computing machine 10 is used to gather electric signal, the control mobile platform 15 of photodetector 9 outputs and makes one-dimensional scanning motion and liquid pump 14 suction samples.
From structural drawing 2 of the present invention and structural drawing 1 comparison of technology formerly, characteristics of the present invention are exactly: be equipped with first conical mirror 11 and second conical mirror 12 on the laser excitation light path between LASER Light Source 1 and the dichronic mirror 3, these two conical mirrors are formed beam converters; Placed multifiber 5 in the sample cell 6; Fiber-optic output face 503 is coated with the ATR Optical film; Sample cell 6 places on the mobile platform 15, and sample cell 6 moves as one-dimensional scanning along the orientation of multifiber 5 with mobile platform 15 under computing machine 10 controls, realizes the scanning of multifiber surface fluorescence signal is detected.
Said first conical mirror 11 and second conical mirror 12 place between the LASER Light Source 1 and dichronic mirror 3 on the laser excitation light path, and the rotation center of the circular conical surface of two conical mirrors 11,12 is positioned on the optical axis OO of laser excitation light path.The front surface of first conical mirror 11 is the plane, and the rear surface is a concave surface.The front surface of second conical mirror 12 is a convex surface, and the rear surface is the plane.The bus of two conical mirror circular conical surfaces and the angle between the plane are θ, as shown in Figure 3.These two conical mirrors 11,12 are formed a beam converter, circular laser beam is transformed into annular laser beams, be that the circular Gaussian beam AA of a is during by 12 outgoing of second conical mirror promptly being incident in radius on first conical mirror, 11 front surfaces, being transformed to inside radius is that r, external radius are the annular beam BB of R, its width (R-r)=a; Inside radius r, the external radius R of annular beam BB is relevant with the distance L between two conical mirrors, 11,12 summits, but its ring width (R-r) remains unchanged, and equals a all the time.As shown in Figure 4.Relation between R and θ, L and the conical mirror refractive index n is determined by following formula: r=sin2 θ [ncos θ/(1-n
2Sin
2θ)
1/2-1] (L/2).Generally, θ is 5 °≤θ≤45 °, and L is 5mm≤L≤20mm.Change a parameter among θ, the L, or change θ and two parameters of L simultaneously, can change the inside/outside diameter size of annular beam BB.
Annular beam BB focuses on the laggard optical fiber 5 of going into through coupled lens 4, and the incident angle of leaving center light far away more is big more.This annular beam can improve the launching efficiency to fluorescence greatly, and the one, the light big more because of incident angle is many more at optical fiber 5 internal reflection number of times, has also just increased the number of times that excites to fluorescent material, thereby can inspire more fluorescent energy; The 2nd, because angle of incidence of light is big more, the penetration depth of its evanescent wave is big more, and the scope that excites that this has also just increased fluorescent material can excite more fluorescent energy equally.
Said optical fiber 5 is multimode jumbo fibers, and wherein a part of covering is removed, and exposes heart yearn 502, and heart yearn 502 surfaces are equipped with biological identification molecule, and the optical fibre core 502 that the surface has biological identification molecule places in the sample cell 6.Sample cell 6 the has been built-in with mark sample 601 of fluorescent dye.When between the sample 601 in the biological identification molecule on optical fibre core 502 surfaces and the sample cell 6 specificity taking place and combine, fluorescent dye also is attached to optical fibre core 502 surfaces together, excites down in laser beam, and fluorescent dye sends fluorescence.
Fiber-optic output face 503 is coated with broadband total reflection dielectric film, is used for excitation laser and fluorescence are reflected back, to improve the receiving efficiency of laser excitation efficient and fluorescence.After laser beam was reflected back to optical fiber 5, the fluorescent dye on excitation fiber heart yearn 502 surfaces made the utilization factor of excitation laser beam improve 1 times again; Fluorescence also is reflected back toward optical fiber 5 and by after optical fiber 5 transmission, enters the fluorescence receiving light path, makes the fluorescence receiving efficiency improve 1 times.So behind optical fiber output section 503 plating broadband total reflection dielectric films, total fluorescence signal intensity that photodetector 9 of the present invention receives is 4 times of technology formerly.
Placed multifiber 5 in the said sample cell 6, the multifiber one dimension is equidistantly arranged, and the central point of each optic fibre input end face is positioned on the straight line, promptly on the X-axis.Different biological identification molecules has been fixed on different optical fibre core surfaces, by the one-dimensional scanning motion of mobile platform 15, can record the concentration of the fluorescent dye on each optical fibre core surface, just the concentration of certain corresponding tested biological substance in the sample cell 6.So concentration of multiple biological substance in the once detectable sample.
Liquid pump 14 is arranged in the sample flow path of the present invention, sample cell 6 has sample inlet 602, sample export 603, under the effect of liquid pump 14, sample can be sucked sample cell 6, the sample that maybe test is finished is discharged sample cell 6, so that change sample, rinsing sample cell 6.
The die course of work of ripple biology sensor of many probe optical fiber Shu of the present invention divided for three steps carried out: the first, the preparation of optical fiber and sample: the multifiber that the heart yearn surface is had biological identification molecule inserts sample cell 6, and liquid pump 14 sucks sample in the sample cell 6 under the control of computing machine 10.The second, the detection of simple optical fiber fluorescence signal: the circular Gaussian beam AA that is sent by LASER Light Source 1 is transformed into annular beam BB through first conical mirror 11 and second conical mirror 12, after dichronic mirror 3 reflection and making 90 ° of light beam working direction deflections, directive coupled lens 4.Coupled lens 4 focuses on the input end face 501 of optical fiber 5 with annular beam BB, laser beam enter optical fiber 5 backs optical fiber 5 in through reflection back arrival output end face 503 repeatedly, be output end face 503 again and reflect by former road and turn back to optical fiber 5.The fluorescent dye on evanescent wave excitation fiber heart yearn 502 surfaces that laser beam produces during multihop propagation in optical fiber 5, fluorescent dye sends fluorescence, and has part to enter optical fiber 5, and after optical fiber 5 transmission, a part penetrates from optical fiber 5 input end faces 501; Another part is transferred to output end face 503, is output end face 503 again and reflects back into optical fiber 5, after optical fiber 5 transmission, also penetrates from input end face 501 again.The fluorescence signal that is penetrated by optical fiber 5 input end faces 501 with directional light directive dichronic mirror 3, sees through dichronic mirror 3 directive fluorescence filter plates 7 behind coupled lens 4 collimations, behind fluorescent optical filter 7 further filtering excitation lasers, be focused lens 8 and focus on diaphragm 13 places.Diaphragm 13 is positioned at the conjugate plane place of optical fiber 5 input end faces 501, and it can filtering optical fiber 5 input end faces 501 veiling glare in addition.Fluorescence signal passes that diaphragm 13 is laggard goes into photodetector 9, is converted into the electric signal that is directly proportional with fluorescence signal intensity, is gathered by computing machine 10 again.The electric signal that computing machine 10 collects is directly proportional with the concentration of optical fiber 5 surperficial measured matters, can obtain the concentration of optical fiber 5 surperficial measured matters by the amplitude of analyzing electric signal.Three, the scanning of multifiber fluorescence signal detects: under the control of computing machine 10, mobile platform 15 is being with multifiber to move as one-dimensional scanning along X-direction, when wherein the input end face central point of an optical fiber overlaps with the focus of coupled lens 4, mobile platform 15 promptly stops to move, and adopts second to go on foot the detection of finishing this optical fiber fluorescence signal.Second step of repetition, the 3rd step, the scanning that then can finish the multifiber fluorescence signal detects.
Fig. 2 is a most preferred embodiment of the present invention, and its concrete structure and statement parameter are as follows:
LASER Light Source 1 is that centre wavelength is the semiconductor laser of 635nm, and output power is 10mW, the radius a=2mm of circular Gaussian beam AA.First, second conical mirror 11 and 12 is made by K9 optical glass, refractive index n=1.51459, its circular conical surface bus and plane included angle θ=30 °, two circular conical surface vertex distance L=14.9mm, the inside radius r=4.5mm of outgoing annular beam BB, external radius R=6.5mm.Coupled lens 4 is made up of two gummed mirrors and a convex-concave simple lens, and focal length is 17.4mm.Annular beam BB is coupled inside and outside aperture, lens 4 focusing backs half-angle and is respectively 14.5 ° and 20.5 °.Optical fiber 5 length overalls are 61mm, optical fibre core 502 long 42mm wherein, the material of optical fibre core 502 is quartzy, diameter is φ 1mm, clad material is an organosilicon, their refractive indexes under used wavelength are respectively 1.51459 and 1.41, so the numerical aperture of optical fiber 5 is 0.367, allowing maximum incident angle is 21.5 °.The wideband enhanced aluminium total reflection film of output end face 503 platings of optical fiber 5 had both reflected excitation laser beam, again reflected fluorescent light.3 pairs of excitation laser total reflections of dichronic mirror, and to the fluorescence total transmissivity.Transmitance≤10 of 7 pairs of excitation laser beam of fluorescent optical filter
-6, and to transmitance 〉=75% of fluorescence.Condenser lens 8 is two gummed mirrors, and focal length is 25mm.Diaphragm 13 is positioned at the focal plane place of condenser lens 8, and its clear aperture is φ 1.5mm.Photodetector 9 is a photomultiplier, is positioned at diaphragm 13 about 3mm place afterwards.
Placed 5 optical fiber in the sample cell 6, one dimension is equidistantly arranged, and the centre distance of adjacent fiber is 2.5mm, and the centre distance of first and five optical fibers input end face is 10mm.Mobile platform 15 is by step motor drive, and its stroke is 15mm, is enough to realize that the scanning to 5 optical fiber detects.The stepping rate of mobile platform 15 is 1mm/s.
During measurement, the biological sample solution of Cy5 fluorescent dye that injected mark in sample cell 6 drives mobile platform 15 then, gathers the fluorescence signal of each optical fiber according to this.Every optical fiber all reaches 10 to the detection sensitivity of Cy5 fluorescent dye solution among the embodiment
-10Mol is 140 times of technology formerly; The detection sensitivity difference of each optical fiber is less than 10% simultaneously.The detection required time that embodiment finishes 5 optical fiber fluorescence signals is 10s, and detection efficiency is 5 times of technology formerly.
Claims (5)
1, a kind of many probe biologic sensor for fast travelling waves of optical fibre include laser excitation light path and fluorescence receiving light path, it is characterized in that:
1. described laser excitation light path, comprise LASER Light Source (1), on the light beam working direction of sending along this LASER Light Source (1), be disposed with first conical mirror (11), second conical mirror (12), dichronic mirror (3), coupled lens (4) and optical fiber (5), the focus of this coupled lens (4) overlaps with the center of the input end face (501) of optical fiber (5);
2. described fluorescence receiving light path from optical fiber (5), is followed successively by coupled lens (4), dichronic mirror (3), fluorescent optical filter (7), condenser lens (8), diaphragm (13), photodetector (9);
The optical axis 00 of 3. described laser excitation light path and the optical axis 0 of fluorescence receiving light path
10
1Mutually vertical, and equal surperficial at 45 with dichronic mirror (3);
4. also has sample flow path, form by sample cell (6), sample inlet (602), sample export (603) and liquid pump (14), arrange multifiber (5) in the described sample cell (6) one-dimensionally equally spacedly, the central point of each optic fibre input end face is positioned on the straight line, promptly on the x axle;
5. also have scan drive system, form by computing machine (10) with the mobile platform (15) of controlled driving, sample cell (6) places on this mobile platform (15);
6. the electric signal that described computing machine (10) is used to gather photodetector (9) the output line data of going forward side by side is handled, and this computing machine (10) is also controlled the work of mobile platform (15) and liquid pump (14), to finish the scanning detection of multifiber fluorescence signal.
2, many probe biologic sensor for fast travelling waves of optical fibre according to claim 1, the front surface that it is characterized in that described first conical mirror (11) is the plane, the rear surface is a concave surface, the front surface of second conical mirror (12) is a convex surface, the rear surface is the plane, the bus of the circular conical surface of two conical mirrors (11,12) and the angle on plane are θ, and the span of this θ is 5 °～45 °, and the span of the distance L between two conical mirrors (11, the 12) summit is 5mm～20mm.
3, many probe biologic sensor for fast travelling waves of optical fibre according to claim 1, it is characterized in that described optical fiber (5) is many root multimode fibers, and a part of covering of every optical fiber is removed, and exposes heart yearn (502), and the surface of every heart yearn (502) is fixed with different biological identification molecules.
4, many probe biologic sensor for fast travelling waves of optical fibre according to claim 1 is characterized in that described optical fiber (5) output end face (503) is coated with the broadband reflection dielectric film.
5, many probe biologic sensor for fast travelling waves of optical fibre according to claim 1 is characterized in that described diaphragm (13) is positioned at the conjugate plane place of optical fiber (5) input end (501).
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100454691C (en) * | 2007-02-12 | 2009-01-21 | 长春理工大学 | Three-dimensional potential well laser |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US7547114B2 (en) | 2007-07-30 | 2009-06-16 | Ylx Corp. | Multicolor illumination device using moving plate with wavelength conversion materials |
| US8502166B2 (en) | 2008-02-04 | 2013-08-06 | Koninklijke Philips N.V. | Molecular diagnostic system based on evanescent illumination and fluorescence |
| CN102341696B (en) | 2009-03-03 | 2013-12-11 | 万迈医疗仪器有限公司 | Detection system and method for high sensitivity fluorescent assays |
| CN101504418B (en) * | 2009-03-18 | 2012-08-08 | 中国人民解放军军事医学科学院微生物流行病研究所 | Bioassay sensor |
| CN101701913B (en) * | 2009-11-20 | 2011-03-30 | 清华大学 | Multi-probe optical fiber evanescent wave biological sensor with all-optical-fiber structure |
| CN103411907B (en) * | 2013-08-27 | 2015-09-02 | 西南大学 | A kind of pole shallow-layer detecting light spectrum probe |
| CN104597279B (en) * | 2013-10-31 | 2018-06-15 | 中国科学院空间科学与应用研究中心 | A kind of ultrahigh speed particle speed measuring system and method |
| CN104614367B (en) * | 2015-02-10 | 2017-12-12 | 华中科技大学 | A kind of Multi-channel optical detecting system |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100454691C (en) * | 2007-02-12 | 2009-01-21 | 长春理工大学 | Three-dimensional potential well laser |
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