CN1299818A

CN1299818A - Tea flavone as one flavone compound from tea

Info

Publication number: CN1299818A
Application number: CN00104568A
Authority: CN
Inventors: 汪健
Original assignee: Individual
Current assignee: Individual
Priority date: 1999-07-23
Filing date: 2000-03-23
Publication date: 2001-06-20

Abstract

The technological process for extracting compound "tea-flavone" from green tea or olong tea includes the following steps: soaking, recovering solvent, extracting tea-polyphenol, catalytic reaction and extraction. The obtained product uses ester type catechin (EGCG, ECG) as its main component, and its content can be up to above 90% (removing caffeine). As compared with existent tea-polyphenol it possesses strongs resistance to oxidation, better stability and higher product purity, and can be dissolved in solvents of water, alcohol, methyl alcohol and ethyl ether, and its aqueous solution is yellow-orange colour. It possesses extensive application fields of beverage, medicine, health-care product, food, chemical and radiation-proofing articles.

Description

A kind of tea flavone as one flavone compound from tea

The invention belongs to a kind of Measurement for Biochemistry of extracting plant effective active composition.

Continuous development along with science and technology, people to the research of tealeaves from previously to the requirement of tea leaf quality, be that color, shape have been developed into from biochemical angle the main component of tealeaves and content are carried out profound research, and developed have anti-oxidant, antitumor, prevent and treat a series of products of functions such as cardiovascular disorder, as tea polysaccharide, tea-polyphenol, catechin (as EGCG, EGC, ECG, EC) etc.One nine six zero years, the Britain biochemist has found the double focusing thing-theoflavin of the catechin that anti-oxidant function is stronger from black tea, but because the content of theoflavin in black tea very low (0.5-1.8%), extraction cost is too high, and theoflavin itself is unstable, therefore, except the standard substance that theoflavin is arranged are sold, but never come out with the form of product.The tea-polyphenol that has formed industrialization is the mixture that catechin and other polyphenolic compound are formed, and its catechin is formed complicated, and the ester catechin relative content is low, and less than 40%, therefore, the medicines and health protection effect of tea-polyphenol is undesirable.Simultaneously, too strong and be easy to produce brown stain because of the bitter taste (being convergency) of tea-polyphenol, be restricted in the application of beverage, healthcare products, medicine and other fields, only in fatty foods, use at present as antioxidant.The monomeric substance of catechin, as NVP-XAA 723 (EGCG), L-Epicatechin gallate (ECG), epigallocatechin (EGC), l-Epicatechol (EC), catechin (C) etc., because the not high and unstable chemcial property of the content of monomer whose in tea-polyphenol, thereby the separation and purification cost is very high, and its product never has extensive industrialization.In a word, the industrialization development in the biochemical field of tealeaves both at home and abroad rests on the tea-polyphenol stage always, and therefore, seeking low-cost high-load stable catechin production technique of extraction and product has become the direction that the tealeaves Biochemical Research is made great efforts.

Show through preliminary search, also from green tea or oolong tea, do not extract the production technique and the product appearance of high-load, that chemical property is stable teas flavonoid substance-ester catechin in the market by acidification reaction.

Purpose of the present invention will be a raw material with green tea or oolong tea exactly, on the basis of tea-polyphenol production technique, tea-polyphenol is carried out catalyzed reaction, thereby produces a kind of compound-teas flavones (being called for short the tea flavones) of new component.

The production technique that the present invention adopts, with low-grade green tea or oolong tea is raw material, and with alcohol-pickled about two hours, recovery of alcohol distillation got medicinal extract, brew medicinal extract with unsaturated salt solution again, the elimination sediment gets filtrate, again filtrate is adjusted to acidic solution, and carries out catalyzed reaction about 2 hours, then with ethyl acetate extraction three times, remove caffeine,, promptly get product at last with extraction liquid vacuum-drying.

The product that this technology that the present invention adopts obtains is to be the teas flavones of main component with the ester catechin, the content of ester catechin can reach more than 90% and (remove caffeine), the main the following aspects of its medicines and health protection effect: anti-ageing, control cardiovascular and cerebrovascular diseases, antitumor, reducing blood-fat, hypoglycemic, anti-bacteria and anti-virus, radioprotective etc.Stability, oxidation-resistance and the ester catechin content of the teas flavones that this technology is extracted are much higher than tea-polyphenol.This technical matters is simple, less investment, and cost is low, and product has purity height, soluble in water, alcohol, methyl alcohol, ether equal solvent, and the aqueous solution shows characteristics such as stable Huang-orange-yellow.The teas flavones that this technology is extracted is because of its distinctive stability, have wide practical use in fields such as beverage, medicine, healthcare products, food, daily-use chemical industry, radioprotective articles for use, as: can be used as the raw material of functional pure tea beverages, the pure product that can be made into Medicines and Health Product capsule, ester catechin can be used as a class medicine or two class medicines, what also can be used as foodstuff additive, makeup and toothpaste protects tooth additive and radioprotective articles for use etc.Become a new milestone in the biochemical development field of tealeaves.

Embodiment: take by weighing the green tea end of 200g, the alcohol 1000ml with 80% soaked 2 hours, crossed the filtering tea grounds and got filtrate 800ml, and concentrated recovered alcohol gets medicinal extract 100ml.In medicinal extract, add 10% Nacl solution 150ml, remove sediment and get filtrate 200ml.Transferring the pH value of filtrate is 4, and 60 degrees centigrade of following catalyzed reactions of temperature 2 hours.Reaction solution extracts three times with 1: 1 ethyl acetate, gets extraction liquid 500ml and removes caffeine.At last that the extraction liquid of gained is dry in vacuum drying oven, promptly get product-teas flavones.Detect through high performance liquid chromatography (HPLC), the composition of the tea flavones product that this technology is extracted is that EGCG content is that 40-68%, ECG content are 14-24% (looking the degree of removing caffeine), content of caffeine is 0.5-26%, that is: the content of " EGCG+ECG+Cafeine " and greater than 85%, other components are: EGC content is that 0.5-3.5%, theoflavin content are 0.5-1.0% or the like.

Claims

1, a kind of is the teas chromocor compound (being called for short the tea flavones) that principal constituent is formed by ester catechin, its production technique feature is: with green tea or oolong tea is that raw material (can be a tea dust, low-grade tea such as tea fragment), after the alcohol-pickled 1.5-3 of 70%-90% hour, the elimination tea grounds, filtrate vacuum concentration recovered alcohol.Gained medicinal extract pours unsaturated common salt aqueous solution, the elimination sediment, and it is acid 1-6.5 that gained filtrate is transferred pH value, carries out under 35-75 ℃ catalyzed reaction 1.5-2.5 hour.Reacted solution is removed caffeine with after 1: 1 the ethyl acetate extraction three times.The vacuum-drying extraction liquid gets product " tea flavones ".

2, teas chromocor compound according to claim 1, wherein two kinds of major ingredient are respectively ester catechin EGCG and ECG, its chemical formula is respectively C ₂₂H ₁₈O ₁₁, C ₂₂H ₁₈O ₁₀, molecular weight is respectively 458 and 442.Its ester catechin is that EGCG is 40-68% at whole tea flavones product proportion, and ECG is 14-24%.Content of caffeine 0.5-26%, that is: the content of " EGCG+ECG+Cafeine " and greater than 85%, all the other several small component content are between 0.1-4.5%.

3, teas chromocor compound according to claim 1, its purposes can be made foodstuff additive, functional pure tea beverages raw material, pharmaceutical raw material, healthcare products, makeup, protect tooth articles for use additive and radioprotective articles for use etc.

4, tea flavones production technique according to claim 1, the ethanol concn that its immersion tealeaves is used is 75-85%, the alcohol consumption is 2-8 a times of tea.

5, tea flavones production technique according to claim 1, the concentration of its used unsaturation salt solution is 1-30%, addition is 0.5-2.5 a times of medicinal extract.

6, tea flavones production technique according to claim 1, its described catalyzed reaction solution acid number is controlled at pH value between 2～4.

7, teas flavones production technique according to claim 1, its catalyzed reaction temperature is 40-60 ℃.

8, tea flavones production technique according to claim 1, the product physical properties is:

Solid is buff powder, and that the aqueous solution is is stable, bright Huang-orange-yellow.