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CN113946057A - Multimode optical fiber dodging device - Google Patents

Multimode optical fiber dodging device Download PDF

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Publication number
CN113946057A
CN113946057A CN202111199087.6A CN202111199087A CN113946057A CN 113946057 A CN113946057 A CN 113946057A CN 202111199087 A CN202111199087 A CN 202111199087A CN 113946057 A CN113946057 A CN 113946057A
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Prior art keywords
dodging
spherical lens
laser
lens
component
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梁倩
陈龙超
王谷丰
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Shenzhen Sailu Medical Technology Co ltd
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Shenzhen Sailu Medical Technology Co ltd
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    • GPHYSICS
    • G02OPTICS
    • G02BOPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/09Beam shaping, e.g. changing the cross-sectional area, not otherwise provided for
    • G02B27/0938Using specific optical elements
    • G02B27/0994Fibers, light pipes
    • GPHYSICS
    • G02OPTICS
    • G02BOPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00Microscopes
    • G02B21/0004Microscopes specially adapted for specific applications
    • G02B21/002Scanning microscopes
    • G02B21/0024Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052Optical details of the image generation
    • G02B21/0076Optical details of the image generation arrangements using fluorescence or luminescence
    • GPHYSICS
    • G02OPTICS
    • G02BOPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00Microscopes
    • G02B21/02Objectives
    • GPHYSICS
    • G02OPTICS
    • G02BOPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/09Beam shaping, e.g. changing the cross-sectional area, not otherwise provided for
    • G02B27/0927Systems for changing the beam intensity distribution, e.g. Gaussian to top-hat
    • GPHYSICS
    • G02OPTICS
    • G02BOPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/09Beam shaping, e.g. changing the cross-sectional area, not otherwise provided for
    • G02B27/0938Using specific optical elements
    • G02B27/095Refractive optical elements
    • G02B27/0955Lenses
    • G02B27/0961Lens arrays

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  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Microscoopes, Condenser (AREA)

Abstract

The invention discloses a multimode optical fiber dodging device which comprises a shaping component, a dodging component and a light condensing element, wherein the shaping component, the dodging component and the light condensing element are sequentially distributed along the direction of an optical axis; the shaping assembly is used for shaping laser emitted by the optical fiber so as to meet the input requirement of the dodging assembly; the dodging component is used for dodging the shaped laser; the light condensing element is used for focusing the laser after light homogenizing; the light condensing element includes a microscope objective. The embodiment of the invention has small volume and strong applicability, can be used by matching with an objective lens, and can be widely applied to the field of optical elements.

Description

Multimode optical fiber dodging device
Technical Field
The invention relates to the field of optical elements, in particular to a multimode optical fiber dodging device.
Background
The existing laser used in the gene sequencing field is generally a multimode fiber output laser with NA0.22, the light intensity distribution is ultrahigh Gaussian distribution, and the intensity distribution of the center and the periphery of a light source is not uniform. The quantum efficiency of the fluorescent group is generally low, and in order to excite the sample at the edge of the visual field to have a sufficient signal-to-noise ratio, the light intensity needs to be adjusted to be large enough, but at the same time, the central light intensity is also increased at the same time, so that the sample can be damaged, and the photo toxicity can cause the photo bleaching of the sample. On the other hand, the existing dodging and shaping optical element on the market at present is generally a structure of a whole set of lens, has large volume and high manufacturing cost, can only homogenize and shape standard Gaussian beams, cannot homogenize specific ultrahigh Gaussian beams, has poor applicability, can not be matched with a microscope objective for use because a light source directly dodges after passing through the dodging and shaping element, and is difficult to ensure that the light intensity at the focal plane of the objective is uniformly distributed.
Disclosure of Invention
In view of the above, an object of the embodiments of the present invention is to provide a multimode optical fiber dodging device, which has a small size and high applicability and can be used with an objective lens.
The embodiment of the invention provides a multimode optical fiber dodging device which comprises a shaping component, a dodging component and a light condensing element, wherein the shaping component, the dodging component and the light condensing element are sequentially distributed along the direction of an optical axis; wherein,
the shaping component is used for shaping the laser emitted by the optical fiber so as to meet the input requirement of the dodging component;
the dodging component is used for dodging the shaped laser;
the light condensing element is used for focusing the laser after light homogenizing; the light condensing element includes a microscope objective.
Optionally, the shaping component includes a first spherical lens and a second spherical lens sequentially distributed along the optical axis direction, and the dodging component includes a first even-order aspheric lens and a second even-order aspheric lens sequentially distributed along the optical axis direction; wherein,
the shaping assembly consists of the first spherical lens and the second spherical lens and is used for reducing the aperture angle of the laser;
and the light homogenizing component is composed of the first even-order aspheric lens and the second even-order aspheric lens and is used for homogenizing the shaped laser.
Optionally, the first spherical lens and the second spherical lens are both plano-convex lenses, and a surface of one side far away from the optical fiber is a plane.
Optionally, the following relationship is satisfied between the first spherical lens and the second spherical lens:
Figure BDA0003304227810000021
wherein λ represents the wavelength of the laser, f1Denotes the focal length of the first spherical lens, f2Denotes a focal length of the second spherical lens, and d denotes a distance between the first spherical lens and the second spherical lens.
Optionally, the focal length of the first spherical lens satisfies the following relationship:
1.4×105λ≤f1≤1.6×105λ
optionally, the focal length of the second spherical lens satisfies the following relationship:
1.8×105λ≤f2≤2×105λ
optionally, the first even aspheric lens and the second even aspheric lens satisfy the following relationship:
Figure BDA0003304227810000022
Figure BDA0003304227810000023
wherein z represents the surface type of the even-order aspherical lens, c represents the curvature, k represents the conic coefficient, and α1Denotes the 2 nd order aspherical coefficient, alpha2Denotes the 4 th order aspherical coefficient, alpha3Denotes the aspherical coefficient of order 6, alpha4Denotes an 8 th order aspherical surface coefficient, and x and y denote coordinate positions of the aspherical surface.
Optionally, the first even-order aspheric lens and the second even-order aspheric lens are made of plastic.
Optionally, the shaping assembly comprises a third spherical lens, the dodging assembly comprises a first powell prism and/or a second powell prism, and when the dodging assembly comprises the first powell prism and the second powell prism, the first powell prism and the second powell prism are perpendicular to each other; wherein,
the shaping assembly is composed of the third spherical lens and is used for collimating laser;
the first Powell prism is used for homogenizing the shaped laser in a first direction;
the second Powell prism is used for homogenizing the shaped laser in a second direction; the first direction and the second direction are perpendicular to each other.
The implementation of the embodiment of the invention has the following beneficial effects: in the embodiment of the invention, laser emitted by an optical fiber is firstly shaped by a shaping component to meet the input requirement of a dodging component, then the shaped laser is dodged by the dodging component, and finally the dodged laser is focused by a light condensing element, wherein the light condensing element comprises a microscope objective; through the combination of the shaping component and the light homogenizing component, the light homogenizing shaping can be carried out on the Gaussian distribution light beam or the ultra-high Gaussian distribution light beam, the light intensity of the center of the homogenized visual field and the edge of the visual field can be homogenized so as to achieve the purpose of uniform illumination, the requirement on the uniformity of a light source is reduced, the size is small, the applicability is strong, the light homogenizing shaping device can be matched with an objective lens to use, the structure is simple, the installation and adjustment difficulty is small, and the realization is easy.
Drawings
FIG. 1 is a block diagram of a multimode optical fiber dodging device according to an embodiment of the present invention;
FIG. 2 is a light path diagram of a first multimode fiber dodging device provided by an embodiment of the invention;
fig. 3 is a speckle pattern of a laser light source provided by an embodiment of the present invention before dodging;
FIG. 4 is a diagram illustrating a distribution of light intensity of a laser source in an x direction before dodging;
FIG. 5 is a spot diagram of a laser source after being homogenized by a first multimode fiber homogenizing device according to an embodiment of the present invention;
FIG. 6 is a light intensity distribution diagram of a laser light source in an x direction after being homogenized by a first multimode fiber homogenizing device according to an embodiment of the present invention;
FIG. 7 is a light path diagram of a second multimode fiber dodging device provided by an embodiment of the invention;
FIG. 8 is a light path diagram of a third multimode fiber dodging device provided by an embodiment of the invention;
FIG. 9 is a spot diagram of a laser source after being homogenized by a second multimode fiber homogenizing device or a third multimode fiber homogenizing device according to an embodiment of the invention;
FIG. 10 is a light intensity distribution diagram in the y-direction of a laser light source after being homogenized by a second multimode fiber homogenizing device according to an embodiment of the present invention;
fig. 11 is a light intensity distribution diagram in the x direction after a laser light source provided by the embodiment of the invention is homogenized by a third multimode fiber homogenizing device.
Detailed Description
The invention is described in further detail below with reference to the figures and the specific embodiments. The step numbers in the following embodiments are provided only for convenience of illustration, the order between the steps is not limited at all, and the execution order of each step in the embodiments can be adapted according to the understanding of those skilled in the art.
As shown in fig. 1, an embodiment of the present invention provides a multimode optical fiber dodging device, including a shaping component, a dodging component and a light condensing element, which are sequentially distributed along an optical axis direction; wherein,
the shaping component is used for shaping the laser emitted by the optical fiber so as to meet the input requirement of the dodging component;
the dodging component is used for dodging the shaped laser;
the light condensing element is used for focusing the laser after light homogenizing; the light condensing element includes a microscope objective.
Specifically, firstly, laser emitted by the optical fiber is shaped by the shaping component, then the shaped laser is homogenized by the light homogenizing component, and finally the homogenized laser is focused by the light condensing element to achieve output light meeting requirements.
It should be noted that the specific shaping function performed by the shaping component needs to be set according to the requirement of the dodging component, the function of the shaping component includes, but is not limited to, reducing the aperture angle of the input laser or collimating the input laser, and the embodiment of the present invention is not limited in particular.
It will be understood by those skilled in the art that the dodging assembly may be a combination of multiple lenses or a single lens, and the specific requirements are set according to the requirements of the output light, and the embodiments of the present invention are not limited in particular.
In some embodiments, as shown in fig. 2, the shaping assembly includes a first spherical lens L1 and a second spherical lens L2 sequentially distributed along the optical axis direction, the light homogenizing assembly includes a first even-order aspheric lens L3 and a second even-order aspheric lens L4 sequentially distributed along the optical axis direction, and the light condensing element is a microscope objective lens L5; wherein,
a shaping assembly consisting of the first spherical lens L1 and the second spherical lens L2 for reducing the aperture angle of the laser light;
and the light homogenizing assembly consists of the first even-order aspheric lens L3 and the second even-order aspheric lens L4 and is used for homogenizing the shaped laser.
Alternatively, referring to fig. 2, the first spherical lens L1 and the second spherical lens L2 are both plano-convex lenses and the surface of the side away from the optical fiber is a plane.
Optionally, the following relationship is satisfied between the first spherical lens and the second spherical lens:
Figure BDA0003304227810000041
wherein λ represents the wavelength of the laser, f1Denotes a focal length, f, of the first spherical lens L12Denotes a focal length of the second spherical lens L2, and d denotes a distance between the first spherical lens L1 and the second spherical lens L2.
It should be noted that if the combined focal length of L1 and L2 is too large, the diopter of the combined lens group is low, and the aperture angle of the light passing through L2 is still large, which results in large aperture of the elements in the subsequent optical path and large volume of the optical system, which is not ideal; if the combined focal length of L1 and L2 is too small, then at least one of the four surfaces of L1 and L2 has a larger curvature, resulting in a larger spherical aberration, which is also undesirable.
Optionally, the focal length of the first spherical lens L1 satisfies the following relationship:
1.4×105λ≤f1≤1.6×105λ
optionally, the focal length of the second spherical lens L2 satisfies the following relationship:
1.8×105λ≤f2≤2×105λ
in addition, f is1And f2The difference of the light intensity of the light passing through the spherical lens L1 and the spherical lens L2 is small, and the light intensity changes gradually, so that the tolerance sensitivity of the system is well influenced.
Optionally, the first even aspheric lens and the second even aspheric lens satisfy the following relationship:
Figure BDA0003304227810000051
Figure BDA0003304227810000052
wherein z represents the surface type of the even-order aspherical lens, c represents the curvature, k represents the conic coefficient, and α1Denotes the 2 nd order aspherical coefficient, alpha2Denotes the 4 th order aspherical coefficient, alpha3Denotes the aspherical coefficient of order 6, alpha4Denotes an 8 th order aspherical surface coefficient, and x and y denote coordinate positions of the aspherical surface.
Optionally, the material of the first even-order aspheric lens and the second even-order aspheric lens is plastic. The first even-order aspheric lens and the second even-order aspheric lens are made of plastic, so that the processing and manufacturing are convenient, and the processing cost is low.
The following describes the multimode fiber dodging device with an embodiment.
The laser wavelength is 532nm, the optical fiber caliber is 0.2 x 0.2mm, and the numerical aperture NA is 0.22. The intensity distribution of the laser light source satisfies the following superss formula:
Figure BDA0003304227810000053
wherein, thetaxySpatial angles in the x and y directions, respectively, I0Is the average intensity of the incident light source. The simulation results of the light source model are shown in fig. 3 and 4, and the light intensity is in a super-Gaussian distribution; fig. 3 shows the spot at 0.5mm behind the exit of the laser fiber, and fig. 4 shows the intensity distribution along the x-direction at 0.5mm behind the exit of the laser fiber.
The multimode fiber dodging device is selected from the device in fig. 2. Wherein, the focal length f of the first spherical lens L11And focal length f of the second spherical lens L22Satisfies the following relationship:
Figure BDA0003304227810000054
focal length f of first spherical lens L11Satisfies the following conditions: f is more than or equal to 76mm1Less than or equal to 82mm, and the focal length f of the second spherical lens L22Satisfies the following conditions: f is not less than 98mm2Less than or equal to 105 mm. The focal length of the microscope objective L5 was 10 mm. The specific parameters of each lens of the device of fig. 2 are shown in table one.
Watch 1
Figure BDA0003304227810000055
In table one, BK7 is borosilicate crown glass, and BK7 has a relatively high hardness and can prevent scratches; the high uniformity, low bubble and impurity content of BK7 in the transmission spectral range 380-2100nm, BK7, and the simple manufacturing and processing techniques make it a good choice for making transmissive optical elements. PMMA (polymethyl methacrylate) is a high molecular polymer, also called as acrylic or organic glass, has the advantages of high transparency, low price, easy machining and the like, and is a glass substitute material frequently used in the prior art.
After the laser emitted by the optical fiber passes through the multimode optical fiber dodging device in fig. 2, light spots on an image surface are as shown in fig. 5 and fig. 6, the light intensity distribution is uniform, and the diameter of the light spot can reach about 1.58 mm.
In some embodiments, as shown in fig. 7, the shaping assembly comprises a third spherical lens L6, the dodging assembly comprises a first powell prism L7 and/or a second powell prism L8, and when the dodging assembly comprises a first powell prism L7 and a second powell prism L8, the first powell prism L7 and the second powell prism L8 are perpendicular to each other; wherein,
the shaping assembly consisting of the third spherical lens L6 is used for collimating laser;
the first Powell prism L7 is used for homogenizing the shaped laser in a first direction;
the second Powell prism L8 is used for homogenizing the shaped laser in a second direction; the first direction and the second direction are perpendicular to each other.
It should be noted that the dodging assembly may include any one of the first powell prism L7 or the second powell prism L8, or both the first powell prism L7 and the second powell prism L8.
It will be understood by those skilled in the art that the first direction may be understood as the y-direction of the coordinate axis and the second direction may be understood as the x-direction of the coordinate axis.
Specifically, as shown in fig. 7, the laser source is collimated after passing through a spherical lens L6, and after passing through a first powell prism L7 in the y direction, the collimated beam is homogenized in the y direction and finally imaged on the sample surface through a microscope objective L9, where the second powell prism L8 corresponds to a piece of plate glass.
Specifically, as shown in fig. 8, the laser source is collimated after passing through a spherical lens L6, and after passing through a second powell prism L8 in the x direction, the collimated beam is homogenized in the x direction and finally imaged on the sample surface through a microscope objective L9, where the first powell prism L7 corresponds to a piece of plate glass.
The multimode fiber dodging device of fig. 7 or fig. 8 is described below in a specific embodiment.
The laser wavelength is 532nm, the optical fiber caliber is 0.2 x 0.2mm, and the numerical aperture NA is 0.22. The intensity distribution of the laser light source satisfies the following superss formula:
Figure BDA0003304227810000061
wherein, thetaxySpatial angles in the x and y directions, respectively, I0Is the average intensity of the incident light source. The simulation results of the light source model are shown in fig. 3 and 4, and the light intensity is in a super-Gaussian distribution; fig. 3 shows the spot at 0.5mm behind the exit of the laser fiber, and fig. 4 shows the intensity distribution along the x-direction at 0.5mm behind the exit of the laser fiber.
The multimode fiber dodging device is selected from the devices in fig. 7 or fig. 8, and specific parameters are shown in table 2.
Watch two
Figure BDA0003304227810000071
When the laser output from the optical fiber is input to the device shown in fig. 7, the light spot on the image surface is as shown in fig. 9, 10 and 11, and the light spot length with uniformity of more than 85% can reach about 1.4 mm.
It should be noted that this embodiment can realize the function of homogenizing and shaping the super-gaussian distribution beam, but under the condition of 85% or more of uniformity, this solution will cause about 1/2 of energy loss, and therefore, it is only suitable for the situation where the energy utilization rate of the illumination light source is not so high.
The implementation of the embodiment of the invention has the following beneficial effects: in the embodiment of the invention, laser emitted by an optical fiber is firstly shaped by a shaping component to meet the input requirement of a dodging component, then the shaped laser is dodged by the dodging component, and finally the dodged laser is focused by a light condensing element, wherein the light condensing element comprises a microscope objective; through the combination of the shaping component and the light homogenizing component, the light homogenizing shaping can be carried out on the Gaussian distribution light beam or the ultra-high Gaussian distribution light beam, the light intensity of the center of the homogenized visual field and the edge of the visual field can be homogenized so as to achieve the purpose of uniform illumination, the requirement on the uniformity of a light source is reduced, the size is small, the applicability is strong, the light homogenizing shaping device can be matched with an objective lens to use, the structure is simple, the installation and adjustment difficulty is small, and the realization is easy.
While the preferred embodiments of the present invention have been illustrated and described, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.

Claims (9)

1. A multimode optical fiber dodging device is characterized by comprising a shaping component, a dodging component and a light condensing element which are sequentially distributed along the direction of an optical axis; wherein,
the shaping component is used for shaping the laser emitted by the optical fiber so as to meet the input requirement of the dodging component;
the dodging component is used for dodging the shaped laser;
the light condensing element is used for focusing the laser after light homogenizing; the light condensing element includes a microscope objective.
2. The multimode optical fiber dodging device according to claim 1, wherein said shaping component comprises a first spherical lens and a second spherical lens sequentially distributed along the optical axis direction, and said dodging component comprises a first even-order aspheric lens and a second even-order aspheric lens sequentially distributed along the optical axis direction; wherein,
the shaping assembly consists of the first spherical lens and the second spherical lens and is used for reducing the aperture angle of the laser;
and the light homogenizing component is composed of the first even-order aspheric lens and the second even-order aspheric lens and is used for homogenizing the shaped laser.
3. The multimode fiber dodging device according to claim 2, wherein the first spherical lens and the second spherical lens are both plano-convex lenses and the surface of the side away from the optical fiber is planar.
4. The multimode fiber dodging device according to claim 3, wherein said first spherical lens and said second spherical lens satisfy the following relationship:
Figure FDA0003304227800000011
wherein λ represents the wavelength of the laser, f1Denotes the focal length of the first spherical lens, f2Denotes a focal length of the second spherical lens, and d denotes a distance between the first spherical lens and the second spherical lens.
5. The multimode fiber dodging device according to claim 4, wherein a focal length of said first spherical lens satisfies the following relationship:
1.4×105λ≤f1≤1.6×105λ
wherein λ represents the wavelength of the laser, f1Represents the focal length of the first spherical lens.
6. The multimode fiber dodging device according to claim 4, wherein a focal length of said second spherical lens satisfies the following relationship:
1.8×105λ≤f2≤2×105λ
wherein λ represents the wavelength of the laser, f2Represents the focal length of the second spherical lens.
7. The multimode fiber dodging device according to claim 2, wherein said first even-order aspheric lens and said second even-order aspheric lens satisfy the following relationship:
Figure FDA0003304227800000021
Figure FDA0003304227800000022
wherein z represents the surface type of the even-order aspherical lens, c represents the curvature, k represents the conic coefficient, and α1Denotes the 2 nd order aspherical coefficient, alpha2Denotes the 4 th order aspherical coefficient, alpha3Denotes the aspherical coefficient of order 6, alpha4Denotes an 8 th order aspherical surface coefficient, and x and y denote coordinate positions of the aspherical surface.
8. The multimode fiber dodging device according to claim 2, wherein the first even-order aspheric lens and the second even-order aspheric lens are made of plastic.
9. The multimode fiber dodging device of claim 1, wherein said shaping component comprises a third spherical lens, said dodging component comprises a first Powell prism and/or a second Powell prism, and when said dodging component comprises a first Powell prism and a second Powell prism, said first Powell prism and said second Powell prism are perpendicular to each other; wherein,
the shaping assembly is composed of the third spherical lens and is used for collimating laser;
the first Powell prism is used for homogenizing the shaped laser in a first direction;
the second Powell prism is used for homogenizing the shaped laser in a second direction; the first direction and the second direction are perpendicular to each other.
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CN115287168B (en) * 2022-08-22 2024-08-16 深圳赛陆医疗科技有限公司 Gene sequencer and use method thereof

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Application publication date: 20220118