CN113702267A - Hemoglobin concentration detection method and blood cell analyzer - Google Patents
Hemoglobin concentration detection method and blood cell analyzer Download PDFInfo
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- 238000001514 detection method Methods 0.000 title claims abstract description 218
- 102000001554 Hemoglobins Human genes 0.000 title claims abstract description 34
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- 210000004027 cell Anatomy 0.000 claims abstract description 105
- 210000004369 blood Anatomy 0.000 claims abstract description 80
- 239000008280 blood Substances 0.000 claims abstract description 80
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- 238000000034 method Methods 0.000 claims abstract description 71
- 239000003219 hemolytic agent Substances 0.000 claims abstract description 43
- 238000002156 mixing Methods 0.000 claims abstract description 23
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- 210000000265 leukocyte Anatomy 0.000 claims description 32
- 206010018910 Haemolysis Diseases 0.000 claims description 15
- 230000008588 hemolysis Effects 0.000 claims description 15
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- 238000004820 blood count Methods 0.000 claims description 7
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Abstract
The invention provides a hemoglobin concentration detection method and a blood cell analyzer, wherein the method comprises the following steps: detecting a first detection sample injected into the reaction cell and obtaining a first HGB value; wherein the first test sample comprises a diluent, a blood sample, a first hemolytic agent; detecting a second detection sample injected into the reaction cell and obtaining a second HGB value; the second detection sample is obtained by adding a second hemolytic agent into the first detection sample of the reaction cell and uniformly mixing; selecting a minimum of the first HGB value and the second HGB value as the HGB value of the blood sample. Because the detection result of the HGB value is higher if the influence of bubbles exists in the detection process, the application proposes that the minimum value is selected from the first HGB value and the second HGB value to be used as the HGB value of the blood sample, so that the influence of the bubbles on the HGB value detection result can be reduced as much as possible, and the accuracy of the HGB detection result is improved.
Description
Technical Field
The application relates to the technical field of blood detection, in particular to a hemoglobin concentration detection method and a blood cell analyzer.
Background
Hematology analyzers can be used to detect hemoglobin concentration (HGB), which is an important parameter for the diagnosis of anemia.
The main process for detecting the HGB value at present is as follows: adding a diluent, a blood sample and a hemolytic agent to a reaction cell, irradiating the reaction cell with a light source, taking the detected voltage value as a sample voltage, adding the diluent to the reaction cell, irradiating the reaction cell with the light source, taking the detected voltage value as a background voltage, and then calculating an HGB value of the blood sample based on the sample voltage and the background voltage.
At present, bubbles may exist in a reaction cell inevitably in the detection process, and the irradiation of a light source to the reaction cell containing a detection sample under the condition that the bubbles exist in the reaction cell can cause the detected sample voltage to be lower, further cause the detection result of an HGB value to be higher, and influence the diagnosis of a doctor on the state of an illness.
Therefore, a scheme is needed to reduce the influence of bubbles on the HGB value detection result and improve the accuracy of the HGB value detection result.
Disclosure of Invention
In view of this, the present application provides a method for detecting hemoglobin concentration and a blood cell analyzer, which reduce the influence of bubbles on the HGB value detection result and improve the accuracy of the HGB value detection result.
In order to achieve the above object, the present invention provides the following technical features:
a method for detecting hemoglobin concentration, comprising:
detecting a first detection sample injected into the reaction cell and obtaining a first HGB value; wherein the first test sample comprises a diluent, a blood sample, and a first hemolytic agent;
detecting a second detection sample injected into the reaction cell and obtaining a second HGB value; the second detection sample is obtained by adding a second hemolytic agent into the first detection sample of the reaction cell and uniformly mixing;
selecting a minimum of the first HGB value and the second HGB value as the HGB value of the blood sample.
Optionally, the detecting a first detection sample injected into the reaction cell and obtaining a first HGB value, and the detecting a second detection sample injected into the reaction cell and obtaining a second HGB value includes:
detecting the diluent injected into the reaction tank and obtaining a background voltage, detecting a first detection sample injected into the reaction tank and obtaining a first sample voltage, and detecting a second detection sample injected into the reaction tank and obtaining a second sample voltage;
and calculating to obtain the first HGB value based on the background voltage and the first sample voltage, and calculating to obtain the second HGB value based on the background voltage and the second sample voltage.
Optionally, the detecting the diluent injected into the reaction cell and obtaining a background voltage, detecting a first detection sample injected into the reaction cell and obtaining a first sample voltage, and detecting a second detection sample injected into the reaction cell and obtaining a second sample voltage; calculating to obtain a first HGB value based on the background voltage and the first sample voltage, and calculating to obtain a second HGB value based on the background voltage and the second sample voltage, wherein the method comprises the following steps:
detecting the diluent injected into the reaction cell and obtaining the background voltage;
after the reaction tank is emptied, detecting a first detection sample injected into the reaction tank and obtaining a first sample voltage, and calculating to obtain a first HGB value based on the background voltage and the first sample voltage;
and detecting a second detection sample injected into the reaction cell, obtaining a second sample voltage, and calculating to obtain the second HGB value based on the background voltage and the second sample voltage.
Optionally, the detecting the diluent injected into the reaction cell and obtaining a background voltage, detecting a first detection sample injected into the reaction cell and obtaining a first sample voltage, and detecting a second detection sample injected into the reaction cell and obtaining a second sample voltage; calculating to obtain a first HGB value based on the background voltage and the first sample voltage, and calculating to obtain a second HGB value based on the background voltage and the second sample voltage, wherein the method comprises the following steps:
detecting a first detection sample injected into the reaction cell and obtaining the first sample voltage;
detecting a second detection sample injected into the reaction cell and obtaining a second sample voltage;
after the reaction tank is emptied, detecting diluent injected into the reaction tank and obtaining the background voltage;
and calculating to obtain the first HGB value based on the background voltage and the first sample voltage, and calculating to obtain the second HGB value based on the background voltage and the second sample voltage.
Optionally, the detecting the diluent injected into the reaction cell and obtaining a background voltage, detecting a first detection sample injected into the reaction cell and obtaining a first sample voltage, and detecting a second detection sample injected into the reaction cell and obtaining a second sample voltage; calculating to obtain a first HGB value based on the background voltage and the first sample voltage, and calculating to obtain a second HGB value based on the background voltage and the second sample voltage, wherein the method comprises the following steps:
detecting the diluent injected into the reaction tank and obtaining a first background voltage;
after the reaction pool is emptied, detecting a first detection sample injected into the reaction pool and obtaining a first sample voltage;
detecting a second detection sample injected into the reaction cell and obtaining a second sample voltage;
after the reaction tank is emptied, detecting the diluent injected into the reaction tank again and obtaining a second background voltage;
selecting the larger of the first and second background voltages as the background voltage;
and calculating to obtain the first HGB value based on the background voltage and the first sample voltage, and calculating to obtain the second HGB value based on the background voltage and the second sample voltage.
Optionally, the calculating the first HGB value based on the background voltage and the first sample voltage includes:
according to the formula
Calculating to obtain the first HGB value;
the calculating the second HGB value based on the background voltage and the second sample voltage comprises:
according to the formula
Calculating to obtain the second HGB value;
wherein the first parameter K1 and the second parameter K2 are both predetermined constants;
the first parameter K1 is determined by the first hemolysis amount, the diluent amount and the blood sample amount;
the second parameter K2 is determined by the first hemolysis dose, the second hemolysis dose, the amount of diluent and the amount of blood sample.
Optionally, before selecting the minimum value of the first HGB value and the second HGB value as the HGB value of the blood sample, the method further comprises:
judging whether bubbles exist in the detection process;
and if bubbles exist in the detection process, selecting the minimum value of the first HGB value and the second HGB value as the HGB value of the blood sample.
Optionally, the determining whether there is a bubble in the detection process includes:
judging whether the absolute value of the difference value between the second HGB value and the first HGB value is larger than a first preset threshold value, if so, determining that bubbles exist in the detection process, and otherwise, determining that no bubbles exist in the detection process;
or,
and judging whether the ratio of the second HGB value to the first HGB value is greater than a second preset threshold value, if so, determining that bubbles exist in the detection process, and otherwise, determining that no bubbles exist in the detection process.
Optionally, the method further includes:
detecting the first detection sample injected into the reaction cell and obtaining leukocyte classification data.
Optionally, the method further includes:
detecting a second test sample injected into the reaction cell and obtaining white blood cell count data.
Alternatively, the first parameter K1 for calculating the first HGB value and the second parameter K2 for calculating the second HGB value are stored in advance.
Optionally, the method for detecting hemoglobin concentration is applied to a blood cell analyzer of an animal model.
A blood cell analyzer, comprising:
a reaction tank;
a diluent supply device for supplying a diluent to the reaction cell;
a sample supply device for supplying a blood sample to the reaction cell;
a hemolytic agent supply device for supplying a first hemolytic agent to the reaction cell to mix with the blood sample and the diluent to obtain a first detection sample, and supplying a second hemolytic agent to the reaction cell to mix with the first detection sample to obtain a second detection sample;
the mixing device is used for respectively mixing the first detection sample or the second detection sample in the reaction tank;
and the detection device is used for detecting a first detection sample in the reaction tank to obtain a first HGB value, detecting a second detection sample in the reaction tank to obtain a second HGB value, and selecting the minimum value of the first HGB value and the second HGB value as the HGB value of the blood sample.
Optionally, the detection apparatus includes an HGB detection module for detecting an HGB value, and the HGB detection module is configured to:
detecting the diluent injected into the reaction tank and obtaining a background voltage, detecting a first detection sample injected into the reaction tank and obtaining a first sample voltage, and detecting a second detection sample injected into the reaction tank and obtaining a second sample voltage;
and calculating to obtain the first HGB value based on the background voltage and the first sample voltage, and calculating to obtain the second HGB value based on the background voltage and the second sample voltage.
Optionally, the detecting device further includes a leukocyte detecting module for detecting leukocytes of a detection sample, and the leukocyte detecting module measures optical information of the first detection sample to perform leukocyte counting on the blood sample.
Optionally, the leukocyte detection module is further configured to determine optical information of the second detection sample to perform leukocyte classification on the blood sample.
Optionally, the blood cell analyzer is applied to a blood cell analyzer of an animal model.
Through the technical means, the following beneficial effects can be realized:
the invention can carry out hemoglobin concentration detection on the blood sample twice in the same reaction tank, and obtain a first HGB value and a second HGB value, and the minimum value of the first HGB value and the second HGB value is selected as the HGB value of the blood sample.
Because the detection result of the HGB value is higher if the influence of bubbles exists in the detection process, the application proposes that the minimum value is selected from the first HGB value and the second HGB value to be used as the HGB value of the blood sample, so that the influence of the bubbles on the HGB value detection result can be reduced as much as possible, and the accuracy of the HGB detection result is improved.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present application, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
Fig. 1 is a flowchart of a first embodiment of a method for detecting hemoglobin concentration according to an embodiment of the present invention;
FIG. 2 is a flowchart illustrating a first embodiment of a method for detecting hemoglobin concentration according to an embodiment of the present invention;
fig. 3 is a flowchart of a first implementation manner of a hemoglobin concentration detection method according to an embodiment of the present invention;
fig. 4 is a flowchart of a second implementation manner of a hemoglobin concentration detection method according to an embodiment of the present invention;
fig. 5 is a flowchart of a third implementation manner of a hemoglobin concentration detection method according to an embodiment of the present invention;
FIG. 6 is a schematic structural diagram of a blood cell analyzer according to an embodiment of the present invention;
fig. 7 is a schematic structural diagram of a leukocyte detection device in a blood cell analyzer according to an embodiment of the present invention.
Detailed Description
The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
The invention provides a method for detecting hemoglobin concentration, which is applied to a blood cell analyzer. Referring to fig. 1, the method comprises the following steps:
step S101: detecting a first detection sample injected into the reaction cell and obtaining a first HGB value; wherein the first test sample comprises a diluent, a blood sample, and a first hemolytic agent.
After a certain type of blood sample is obtained by the blood cell analyzer, the proportion of the blood sample, the first hemolytic agent and the diluent is determined, the blood sample, the first solution and the diluent are injected into the reaction tank according to the proportion, and a first detection sample is obtained after uniform mixing. And detecting the concentration of the hemoglobin based on the first detection sample of the reaction cell to obtain a first HGB value of the blood sample.
Optionally, the first test sample is further used for performing a white blood cell classification operation, that is, detecting the first test sample injected into the reaction cell and obtaining white blood cell classification data.
Step S102: detecting a second detection sample injected into the reaction cell and obtaining a second HGB value; and the second detection sample is obtained by adding a second hemolytic agent into the first detection sample of the reaction cell and uniformly mixing.
And determining the specified proportion of the second hemolytic agent on the basis of the proportion of the blood sample, the first hemolytic agent and the diluent, adding the second hemolytic agent in the specified proportion to the reaction cell on the basis of the first detection sample contained in the reaction cell, and uniformly mixing to obtain a second detection sample. And detecting the concentration of the hemoglobin based on a second detection sample of the reaction cell to obtain a second HGB value of the blood sample. In addition, the second hemolytic agent can be added simultaneously with the diluent in a proper proportion.
Optionally, the second test sample is further used for performing a white blood cell count operation, i.e. detecting the second test sample injected into the reaction cell and obtaining white blood cell count data.
Step S103: selecting a minimum of the first HGB value and the second HGB value as the HGB value of the blood sample. It should be noted that the hemoglobin concentration detection apparatus (i.e., the blood cell analyzer) is calibrated by a calibrator at the time of shipment, so that the results of the first HGB value and the second HGB value measured by the hemoglobin concentration detection apparatus coincide with each other. Therefore, when the first HGB value and the second HGB value do not match each other, the error is not caused by the hemoglobin concentration detection device, but is influenced by the air bubbles.
The invention provides a second detection method of hemoglobin concentration, which is applied to a blood cell analyzer. And a process of judging whether bubbles exist in the detection process is added on the basis of the first embodiment.
Referring to fig. 2, the method comprises the following steps:
step S201: detecting a first detection sample injected into the reaction cell and obtaining a first HGB value; wherein the first test sample comprises a diluent, a blood sample, and a first hemolytic agent.
After a certain type of blood sample is obtained by the blood cell analyzer, the proportion of the blood sample, the first hemolytic agent and the diluent is determined, the blood sample, the first solution and the diluent are injected into the reaction tank according to the proportion, and a first detection sample is obtained after uniform mixing. And detecting the concentration of the hemoglobin based on the first detection sample of the reaction cell to obtain a first HGB value of the blood sample.
Optionally, the first test sample is further used for performing a white blood cell classification operation, that is, detecting the first test sample injected into the reaction cell and obtaining white blood cell classification data.
Step S202: detecting a second detection sample injected into the reaction cell and obtaining a second HGB value; and the second detection sample is obtained by adding a second hemolytic agent into the first detection sample of the reaction cell and uniformly mixing.
And determining the specified proportion of the second hemolytic agent on the basis of the proportion of the blood sample, the first hemolytic agent and the diluent, adding the second hemolytic agent in the specified proportion to the reaction cell on the basis of the first detection sample contained in the reaction cell, and uniformly mixing to obtain a second detection sample. And detecting the concentration of the hemoglobin based on a second detection sample of the reaction cell to obtain a second HGB value of the blood sample.
Optionally, the second test sample is further used for performing a white blood cell count operation, i.e. detecting the second test sample injected into the reaction cell and obtaining white blood cell count data.
Step S203: judging whether bubbles exist in the detection process; if yes, the process proceeds to step S204.
If no air bubble is generated in the detection process, one HGB value can be randomly selected from the first HGB value and the second HGB value to serve as the HGB value of the blood sample.
The embodiment provides two implementation modes for judging whether bubbles exist in the detection process:
the first implementation mode comprises the following steps: and judging whether the absolute value of the difference value between the second HGB value and the first HGB value is greater than a first preset threshold value, if so, determining that bubbles exist in the detection process, and otherwise, determining that no bubbles exist in the detection process.
And calculating the absolute value of the difference value between the second HGB value and the first HGB value, and judging whether the absolute value of the difference value between the second HGB value and the first HGB value is greater than a first preset threshold value.
And if the absolute value of the difference between the second HGB value and the first HGB value is not larger than a first preset threshold value, the difference between the first HGB value and the second HGB value is not large (caused by test errors), determining that no air bubbles exist in the detection process, otherwise, determining that air bubbles exist in the detection process.
The second implementation mode comprises the following steps: and judging whether the ratio of the second HGB value to the first HGB value is greater than a second preset threshold value, if so, determining that bubbles exist in the detection process, and otherwise, determining that no bubbles exist in the detection process.
And calculating the ratio of the second HGB value to the first HGB value, and judging whether the ratio of the second HGB value to the first HGB value is greater than a second preset threshold value.
And if the ratio of the second HGB value to the first HGB value is greater than a second preset threshold value, determining that bubbles exist in the detection process, otherwise, determining that no bubbles exist in the detection process.
Step S204: selecting a minimum of the first HGB value and the second HGB value as the HGB value of the blood sample.
Through the technical means, the following beneficial effects can be realized:
the invention can carry out hemoglobin concentration detection on the blood sample twice in the same reaction tank, and obtain a first HGB value and a second HGB value, and the minimum value of the first HGB value and the second HGB value is selected as the HGB value of the blood sample.
Because the detection result of the HGB value is higher if the influence of bubbles exists in the detection process, the application proposes that the minimum value is selected from the first HGB value and the second HGB value to be used as the HGB value of the blood sample, so that the influence of the bubbles on the HGB value detection result can be reduced as much as possible, and the accuracy of the HGB detection result is improved.
According to an embodiment provided by the present invention, the steps S101 and S102 in the first embodiment and the steps S201 and S202 in the second embodiment may specifically include the following operations:
detecting the diluent injected into the reaction tank and obtaining a background voltage, detecting a first detection sample injected into the reaction tank and obtaining a first sample voltage, and detecting a second detection sample injected into the reaction tank and obtaining a second sample voltage; and calculating to obtain the first HGB value based on the background voltage and the first sample voltage, and calculating to obtain the second HGB value based on the background voltage and the second sample voltage.
In the first embodiment, the steps S101 and S102 include three implementation manners, and the following details are described in one-to-three implementation manners.
The first implementation mode comprises the following steps: the background voltage is detected at the beginning of the hemoglobin concentration measurement.
Referring to fig. 3, a first implementation includes the following steps:
step S301: detecting the diluent injected into the reaction cell and obtaining the background voltage.
And injecting a diluent into the reaction tank, and detecting the background voltage of the diluent for calculating the first HGB value and the second HGB value subsequently.
Step S302: and detecting a first detection sample injected into the reaction tank after the reaction tank is emptied, obtaining the first sample voltage, and calculating to obtain the first HGB value based on the background voltage and the first sample voltage.
After detecting the background voltage, the diluent in the reaction cell needs to be emptied so as not to affect the detection of the blood sample.
After a certain type of blood sample is obtained by the blood cell analyzer, the proportion of the blood sample, the first hemolytic agent and the diluent is determined, the blood sample, the first solution and the diluent are injected into the reaction tank according to the proportion, and a first detection sample is obtained after uniform mixing.
And starting a light source, irradiating the reaction cell by adopting the light source and obtaining a first sample voltage of the first detection sample. And calculating to obtain the first HGB value based on the background voltage and the first sample voltage, wherein the calculation formula is as follows:
wherein the first parameter K1 is a predetermined constant, and the first parameter K1 is determined by the first amount of hemolysis, the amount of diluent, and the amount of blood sample.
Step S303: and detecting a second detection sample injected into the reaction cell, obtaining a second sample voltage, and calculating to obtain the second HGB value based on the background voltage and the second sample voltage.
And determining the specified proportion of the second hemolytic agent on the basis of the proportion of the blood sample, the first hemolytic agent and the diluent, adding the second hemolytic agent in the specified proportion to the reaction cell on the basis of the first detection sample contained in the reaction cell, and uniformly mixing to obtain a second detection sample.
And starting the light source, irradiating the reaction cell by adopting the light source and obtaining a second sample voltage of a second detection sample. And calculating to obtain the second HGB value based on the background voltage and the second sample voltage, wherein the calculation formula is as follows:
wherein the second parameter K2 is a predetermined constant; the second parameter K2 is determined from the first hemolysis dose, the second hemolysis dose, the amount of diluent and the amount of blood sample.
Optionally, the hematology analyzer prestores a first parameter K1 for calculating a first HGB value, and a second parameter K2 for calculating a second HGB value.
The second implementation mode comprises the following steps: the background voltage is detected at the end of performing the hemoglobin concentration detection.
Referring to fig. 4, a second implementation includes the following steps:
step S401: detecting a first detection sample injected into the reaction cell and obtaining the first sample voltage.
After a certain type of blood sample is obtained by the blood cell analyzer, the proportion of the blood sample, the first hemolytic agent and the diluent is determined, the blood sample, the first solution and the diluent are injected into the reaction tank according to the proportion, and a first detection sample is obtained after uniform mixing. And starting a light source, irradiating the reaction cell by adopting the light source and obtaining a first sample voltage of the first detection sample.
Step S402: detecting a second detection sample injected into the reaction cell and obtaining the second sample voltage.
And determining the specified proportion of the second hemolytic agent on the basis of the proportion of the blood sample, the first hemolytic agent and the diluent, adding the second hemolytic agent in the specified proportion to the reaction cell on the basis of the first detection sample contained in the reaction cell, and uniformly mixing to obtain a second detection sample. And starting the light source, irradiating the reaction cell by adopting the light source and obtaining a second sample voltage of a second detection sample.
Step S403: and after the reaction tank is emptied, detecting the diluent injected into the reaction tank and obtaining the background voltage.
And emptying the second detection sample in the reaction tank, injecting diluent into the reaction tank, and detecting the background voltage of the diluent for subsequent calculation of the first HGB value and the second HGB value.
Step S404: and calculating to obtain the first HGB value based on the background voltage and the first sample voltage, and calculating to obtain the second HGB value based on the background voltage and the second sample voltage.
And calculating to obtain the first HGB value based on the background voltage and the first sample voltage, wherein the calculation formula is as follows:
wherein the first parameter K1 is a predetermined constant, and the first parameter K1 is determined by the first amount of hemolysis, the amount of diluent, and the amount of blood sample.
And calculating to obtain the second HGB value based on the background voltage and the second sample voltage, wherein the calculation formula is as follows:
wherein the second parameter K2 is a predetermined constant; the second parameter K2 is determined from the first hemolysis dose, the second hemolysis dose, the amount of diluent and the amount of blood sample.
Optionally, the hematology analyzer prestores a first parameter K1 for calculating a first HGB value, and a second parameter K2 for calculating a second HGB value.
The third implementation mode comprises the following steps: the background voltage is detected at the beginning and end of the hemoglobin concentration measurement.
Referring to fig. 5, a third implementation includes the following steps:
step S501: detecting the diluent injected into the reaction cell and obtaining a first background voltage.
The method comprises the steps of firstly injecting diluent into a reaction tank, and detecting a first background voltage of the diluent.
Step S502: and detecting a first detection sample injected into the reaction cell after the reaction cell is emptied, and obtaining the first sample voltage.
After detecting the background voltage, the diluent in the reaction cell needs to be emptied so as not to affect the detection of the blood sample.
After a certain type of blood sample is obtained by the blood cell analyzer, the proportion of the blood sample, the first hemolytic agent and the diluent is determined, the blood sample, the first solution and the diluent are injected into the reaction tank according to the proportion, and a first detection sample is obtained after uniform mixing. And starting a light source, irradiating the reaction cell by adopting the light source and obtaining a first sample voltage of the first detection sample.
Step S503: detecting a second detection sample injected into the reaction cell and obtaining the second sample voltage.
And determining the specified proportion of the second hemolytic agent on the basis of the proportion of the blood sample, the first hemolytic agent and the diluent, adding the second hemolytic agent in the specified proportion to the reaction cell on the basis of the first detection sample contained in the reaction cell, and uniformly mixing to obtain a second detection sample. And starting the light source, irradiating the reaction cell by adopting the light source and obtaining a second sample voltage of a second detection sample.
Step S504: and after the reaction tank is emptied, detecting the diluent injected into the reaction tank again and obtaining a second background voltage.
And emptying the second detection sample in the reaction tank, injecting diluent into the reaction tank, and detecting a second background voltage of the diluent.
Step S505: selecting the larger of the first and second background voltages as the background voltage.
In order to be more accurate, in this embodiment, two detection operations are also performed on the background voltage, so as to eliminate the influence of bubbles mixed in the background voltage detection process on the detection result of the hemoglobin concentration.
From the calculation formula of hemoglobin concentration, it is known that the background voltage is a numerator, and in the case of bubbles, the HGB value is lowered due to lowering of the local voltage. The larger of the first background voltage and the second background voltage is selected as the background voltage to eliminate the influence of the bubbles as much as possible.
Step S506: and calculating to obtain the first HGB value based on the background voltage and the first sample voltage, and calculating to obtain the second HGB value based on the background voltage and the second sample voltage.
And calculating to obtain the first HGB value based on the background voltage and the first sample voltage, wherein the calculation formula is as follows:
wherein the first parameter K1 is a predetermined constant, and the first parameter K1 is determined by the first amount of hemolysis, the amount of diluent, and the amount of blood sample.
And calculating to obtain the second HGB value based on the background voltage and the second sample voltage, wherein the calculation formula is as follows:
wherein the second parameter K2 is a predetermined constant; the second parameter K2 is determined from the first hemolysis dose, the second hemolysis dose, the amount of diluent and the amount of blood sample.
Optionally, the hematology analyzer prestores a first parameter K1 for calculating a first HGB value, and a second parameter K2 for calculating a second HGB value.
Optionally, the method for detecting hemoglobin concentration provided by the present application can be applied to a blood cell analyzer of an animal model to measure hemoglobin concentration of an animal.
Referring to fig. 6, the present invention provides a blood cell analyzer including:
a reaction tank;
a diluent supply device for supplying a diluent to the reaction cell;
a sample supply device for supplying a blood sample to the reaction cell;
a hemolytic agent supply device for supplying a first hemolytic agent to the reaction cell to mix with the blood sample and the diluent to obtain a first detection sample, and supplying a second hemolytic agent to the reaction cell to mix with the first detection sample to obtain a second detection sample;
and the blending device is used for respectively blending the first detection sample or the second detection sample in the reaction tank.
Light source means for emitting a light source.
And the detection device is used for detecting a first detection sample in the reaction tank to obtain a first HGB value, detecting a second detection sample in the reaction tank to obtain a second HGB value, and selecting the minimum value of the first HGB value and the second HGB value as the HGB value of the blood sample.
And a control device in communication connection with the diluent supply device, the sample supply device, the hemolytic agent supply device, the mixing device, the light source device, and the detection device.
The control device is used for controlling the diluent supply device, the sample supply device, the hemolytic agent supply device and the blending device, preparing the diluent, the first detection sample and the second detection sample, controlling the light source device to be started after the diluent is prepared, controlling the light source device to be started after the first detection sample is prepared, and controlling the light source device to be started after the second detection sample is prepared, so that the detection device can detect the first HGB value and the second HGB value.
The detection device comprises an HGB detection module for detecting the HGB value.
The HGB detection module is used for:
detecting the diluent injected into the reaction tank and obtaining a background voltage, detecting a first detection sample injected into the reaction tank and obtaining a first sample voltage, and detecting a second detection sample injected into the reaction tank and obtaining a second sample voltage;
and calculating to obtain the first HGB value based on the background voltage and the first sample voltage, and calculating to obtain the second HGB value based on the background voltage and the second sample voltage.
Specific implementation of the HGB detection module in the blood cell analyzer can be detailed in the embodiments shown in fig. 1 to 5, and will not be described herein again.
Optionally, the blood cell analyzer is applied to a blood cell analyzer of an animal model.
The detection device also comprises a leucocyte detection module for detecting leucocytes of the detection sample.
The leukocyte detection module measures optical information of the first detection sample to perform a leukocyte count on the blood sample.
The leukocyte detection module is further configured to determine optical information of the second detection sample to perform leukocyte classification on the blood sample.
Referring to fig. 7, the present invention provides a schematic structural view of a leukocyte detection device. The white blood cell detecting apparatus includes an optical detecting part 131, and the optical detecting part 131 has a light source 1311, a beam shaping member 1312, a flow cell 1313, and a forward scattered light detector 1314, which are arranged in a line in this order. On one side of flow chamber 1313, a dichroic mirror 1316 is arranged at an angle of 45 ° to the straight line. A portion of the side light emitted by blood cells in flow chamber 1313 is transmitted through dichroic mirror 1316 and captured by fluorescence detector 1315 disposed behind dichroic mirror 1316 at a 45 ° angle to dichroic mirror 1316, while another portion of the side light is reflected by dichroic mirror 1316 and captured by side scatter detector 1317 disposed in front of dichroic mirror 1316 at a 45 ° angle to dichroic mirror 1316. From the forward scattered light signal captured by forward scatter light detector 1314, the side scattered light signal captured by side scatter light detector 1317, and the fluorescence signal captured by fluorescence detector 1315, leukocytes in the blood sample can be counted and classified.
Through the technical means, the following beneficial effects can be realized:
the invention can carry out hemoglobin concentration detection on the blood sample twice in the same reaction tank, and obtain a first HGB value and a second HGB value, and the minimum value of the first HGB value and the second HGB value is selected as the HGB value of the blood sample.
Because the detection result of the HGB value is higher if the influence of bubbles exists in the detection process, the application proposes that the minimum value is selected from the first HGB value and the second HGB value to be used as the HGB value of the blood sample, so that the influence of the bubbles on the HGB value detection result can be reduced as much as possible, and the accuracy of the HGB detection result is improved.
The functions described in the method of the present embodiment, if implemented in the form of software functional units and sold or used as independent products, may be stored in a storage medium readable by a computing device. Based on such understanding, part of the contribution to the prior art of the embodiments of the present application or part of the technical solution may be embodied in the form of a software product stored in a storage medium and including several instructions for causing a computing device (which may be a personal computer, a server, a mobile computing device or a network device) to execute all or part of the steps of the method described in the embodiments of the present application. And the aforementioned storage medium includes: a U-disk, a removable hard disk, a Read-Only Memory (ROM), a Random Access Memory (RAM), a magnetic disk or an optical disk, and other various media capable of storing program codes.
The embodiments are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same or similar parts among the embodiments are referred to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present application. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the application. Thus, the present application is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (17)
1. A method for detecting hemoglobin concentration, comprising:
detecting a first detection sample injected into the reaction cell and obtaining a first HGB value; wherein the first test sample comprises a diluent, a blood sample, a first hemolytic agent;
detecting a second detection sample injected into the reaction cell and obtaining a second HGB value; the second detection sample is obtained by adding a second hemolytic agent into the first detection sample of the reaction cell and uniformly mixing;
selecting a minimum of the first HGB value and the second HGB value as the HGB value of the blood sample.
2. The method of claim 1, wherein the detecting a first test sample injected into the reaction cell and obtaining a first HGB value, and wherein the detecting a second test sample injected into the reaction cell and obtaining a second HGB value comprises:
detecting the diluent injected into the reaction tank and obtaining a background voltage, detecting a first detection sample injected into the reaction tank and obtaining a first sample voltage, and detecting a second detection sample injected into the reaction tank and obtaining a second sample voltage;
and calculating to obtain the first HGB value based on the background voltage and the first sample voltage, and calculating to obtain the second HGB value based on the background voltage and the second sample voltage.
3. The method of claim 2, wherein the detecting the diluent injected into the reaction cell and obtaining a background voltage, detecting a first detection sample injected into the reaction cell and obtaining a first sample voltage, detecting a second detection sample injected into the reaction cell and obtaining a second sample voltage; calculating to obtain a first HGB value based on the background voltage and the first sample voltage, and calculating to obtain a second HGB value based on the background voltage and the second sample voltage, wherein the method comprises the following steps:
detecting the diluent injected into the reaction cell and obtaining the background voltage;
after the reaction tank is emptied, detecting a first detection sample injected into the reaction tank and obtaining a first sample voltage, and calculating to obtain a first HGB value based on the background voltage and the first sample voltage;
and detecting a second detection sample injected into the reaction cell, obtaining a second sample voltage, and calculating to obtain the second HGB value based on the background voltage and the second sample voltage.
4. The method of claim 2, wherein the detecting the diluent injected into the reaction cell and obtaining a background voltage, detecting a first detection sample injected into the reaction cell and obtaining a first sample voltage, detecting a second detection sample injected into the reaction cell and obtaining a second sample voltage; calculating to obtain a first HGB value based on the background voltage and the first sample voltage, and calculating to obtain a second HGB value based on the background voltage and the second sample voltage, wherein the method comprises the following steps:
detecting a first detection sample injected into the reaction cell and obtaining the first sample voltage;
detecting a second detection sample injected into the reaction cell and obtaining a second sample voltage;
after the reaction tank is emptied, detecting diluent injected into the reaction tank and obtaining the background voltage;
and calculating to obtain the first HGB value based on the background voltage and the first sample voltage, and calculating to obtain the second HGB value based on the background voltage and the second sample voltage.
5. The method of claim 2, wherein the detecting the diluent injected into the reaction cell and obtaining a background voltage, detecting a first detection sample injected into the reaction cell and obtaining a first sample voltage, detecting a second detection sample injected into the reaction cell and obtaining a second sample voltage; calculating to obtain a first HGB value based on the background voltage and the first sample voltage, and calculating to obtain a second HGB value based on the background voltage and the second sample voltage, wherein the method comprises the following steps:
detecting the diluent injected into the reaction tank and obtaining a first background voltage;
after the reaction pool is emptied, detecting a first detection sample injected into the reaction pool and obtaining a first sample voltage;
detecting a second detection sample injected into the reaction cell and obtaining a second sample voltage;
after the reaction tank is emptied, detecting the diluent injected into the reaction tank again and obtaining a second background voltage;
selecting the larger of the first and second background voltages as the background voltage;
and calculating to obtain the first HGB value based on the background voltage and the first sample voltage, and calculating to obtain the second HGB value based on the background voltage and the second sample voltage.
6. The method of any one of claims 2 to 5,
said calculating said first HGB value based on said background voltage and said first sample voltage comprises:
according to the formula
Calculating to obtain the first HGB value;
the calculating the second HGB value based on the background voltage and the second sample voltage comprises:
according to the formula
Calculating to obtain the second HGB value;
wherein the first parameter K1 and the second parameter K2 are both predetermined constants;
the first parameter K1 is determined by the first hemolysis amount, the diluent amount and the blood sample amount;
the second parameter K2 is determined by the first hemolysis dose, the second hemolysis dose, the amount of diluent and the amount of blood sample.
7. The method of claim 1, further comprising, prior to selecting a minimum of the first HGB value and the second HGB value as the HGB value of the blood sample:
judging whether bubbles exist in the detection process;
and if bubbles exist in the detection process, selecting the minimum value of the first HGB value and the second HGB value as the HGB value of the blood sample.
8. The method of claim 7, wherein said determining whether a bubble is present during the detection process comprises:
judging whether the absolute value of the difference value between the second HGB value and the first HGB value is larger than a first preset threshold value, if so, determining that bubbles exist in the detection process, and otherwise, determining that no bubbles exist in the detection process;
or,
and judging whether the ratio of the second HGB value to the first HGB value is greater than a second preset threshold value, if so, determining that bubbles exist in the detection process, and otherwise, determining that no bubbles exist in the detection process.
9. The method of claim 1, further comprising:
detecting the first detection sample injected into the reaction cell and obtaining leukocyte classification data.
10. The method of claim 1, further comprising:
detecting a second test sample injected into the reaction cell and obtaining white blood cell count data.
11. The method of claim 6,
the first parameter K1 for calculating the first HGB value and the second parameter K2 for calculating the second HGB value are stored in advance.
12. The method of claim 1,
the method for detecting the concentration of the hemoglobin is applied to a blood cell analyzer of an animal model.
13. A blood cell analyzer, comprising:
a reaction tank;
a diluent supply device for supplying a diluent to the reaction cell;
a sample supply device for supplying a blood sample to the reaction cell;
a hemolytic agent supply device for supplying a first hemolytic agent to the reaction cell to mix with the blood sample and the diluent to obtain a first detection sample, and supplying a second hemolytic agent to the reaction cell to mix with the first detection sample to obtain a second detection sample;
the mixing device is used for respectively mixing the first detection sample or the second detection sample in the reaction tank;
and the detection device is used for detecting a first detection sample in the reaction tank to obtain a first HGB value, detecting a second detection sample in the reaction tank to obtain a second HGB value, and selecting the minimum value of the first HGB value and the second HGB value as the HGB value of the blood sample.
14. The hematology analyzer of claim 13, wherein the detection device includes an HGB detection module for detecting an HGB value, the HGB detection module for:
detecting the diluent injected into the reaction tank and obtaining a background voltage, detecting a first detection sample injected into the reaction tank and obtaining a first sample voltage, and detecting a second detection sample injected into the reaction tank and obtaining a second sample voltage;
and calculating to obtain the first HGB value based on the background voltage and the first sample voltage, and calculating to obtain the second HGB value based on the background voltage and the second sample voltage.
15. The blood cell analyzer of claim 13 or 14, wherein the detecting means further comprises a white blood cell detecting module for detecting white blood cells of the detection sample, and the white blood cell detecting module measures optical information of the first detection sample to perform white blood cell counting on the blood sample.
16. The blood cell analyzer of claim 15, wherein the leukocyte measurement module is further configured to determine optical information of the second test sample to perform leukocyte classification on the blood sample.
17. The method of claim 13,
the blood cell analyzer is applied to a blood cell analyzer of an animal model.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020034824A1 (en) * | 2000-09-18 | 2002-03-21 | Mitsuru Abo | Blood cell detector, blood analyzer and blood analyzing method using the detector |
| CN101246113A (en) * | 2007-02-14 | 2008-08-20 | 深圳迈瑞生物医疗电子股份有限公司 | Monitoring method for hemoglobin test result |
| CN101470109A (en) * | 2007-12-25 | 2009-07-01 | 深圳迈瑞生物医疗电子股份有限公司 | Method for improving accuracy of blood sample leukocyte classifying result |
| WO2015007102A1 (en) * | 2013-07-16 | 2015-01-22 | 成都深迈瑞医疗电子技术研究院有限公司 | Blood analysis method, control device, and blood cell analyzer |
-
2020
- 2020-05-22 CN CN202010441645.4A patent/CN113702267A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020034824A1 (en) * | 2000-09-18 | 2002-03-21 | Mitsuru Abo | Blood cell detector, blood analyzer and blood analyzing method using the detector |
| CN101246113A (en) * | 2007-02-14 | 2008-08-20 | 深圳迈瑞生物医疗电子股份有限公司 | Monitoring method for hemoglobin test result |
| CN101470109A (en) * | 2007-12-25 | 2009-07-01 | 深圳迈瑞生物医疗电子股份有限公司 | Method for improving accuracy of blood sample leukocyte classifying result |
| WO2015007102A1 (en) * | 2013-07-16 | 2015-01-22 | 成都深迈瑞医疗电子技术研究院有限公司 | Blood analysis method, control device, and blood cell analyzer |
| US20160202246A1 (en) * | 2013-07-16 | 2016-07-14 | Chengdu Shen Mindray Medical Electronics T | Blood analysis method, control device, and blood cell analyzer |
Non-Patent Citations (1)
| Title |
|---|
| 徐仁秀, 《临床医药文献杂志》, vol. 4, no. 30, 30 April 2017 (2017-04-30), pages 5858 - 5859 * |
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