CN113504205A - Method for identifying stable components in macroalgae-derived dissolved organic carbon and application - Google Patents
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Abstract
本发明属于海藻技术领域，公开了一种大型海藻藻源溶解有机碳中的稳定组分鉴定方法及应用，所述大型海藻藻源溶解有机碳中的稳定组分鉴定方法包括：进行大型海藻藻源DOC的活性与惰性评估；进行藻源DOC的荧光组分鉴定；进行藻源DOC的分子组成鉴定。本发明通过将大型海藻藻源DOC的活性与惰性评估、藻源DOC的荧光组分鉴定和藻源DOC的分子组成鉴定三部分内容结合，可实现对大型海藻藻源DOC的稳定性组分评估，并最终实现对藻源DOC中的惰性DOC进行定性和定量评估，有助于突破目前对海藻贡献的水体碳汇难以评估的技术“瓶颈”，对未来实现量化海洋碳交易体系、推动大型海藻碳汇产业发展具有重要的应用价值和科学意义。
The invention belongs to the technical field of seaweed, and discloses a method and application for identifying stable components in dissolved organic carbon from a macroalgae source. The method for identifying stable components in dissolved organic carbon from a macroalgae source includes: Activity and inertness evaluation of source DOC; identification of fluorescent components of algal DOC; identification of molecular composition of algal DOC. The present invention can realize the stability component evaluation of macroalgae algae DOC by combining the activity and inertness evaluation of macroalgae-derived DOC, the identification of fluorescent components of algae-derived DOC, and the identification of molecular composition of algae-derived DOC. , and finally realize the qualitative and quantitative evaluation of the inert DOC in the algae source DOC, which will help to break through the current technical "bottleneck" that is difficult to evaluate the water carbon sink contributed by the seaweed. The development of carbon sink industry has important application value and scientific significance.
Description
Technical Field
The invention belongs to the technical field of seaweed, and particularly relates to a method for identifying a stable component dissolved in organic carbon from a macroalgae source and application thereof.
Background
Presently, macroalgae, a plant with very high productivity in the ocean, are usedThe maximum net primary productivity reaches 2.9Pg C yr-1. Which fixes carbon through photosynthesis during growth, releases 20-40% of primary productivity as Dissolved Organic Carbon (DOC) into water. The DOC of algae source in the water body has very complex components and has great difference in stability. The DOC component with high activity can be quickly utilized by microorganisms, and the DOC component with high stability (namely inert DOC) is difficult to utilize and convert by the microorganisms due to high inertization degree, can be stored in seawater for a long time, and becomes an important contribution of large-scale seaweed to the ocean stability DOC reservoir.
In the ocean, the DOC released by macroalgae contains many highly active components that can be quickly absorbed by microbes and enter the micro food circle. According to the marine "micro-biochar pump" (MCP) theory, the highly active dissolved organic carbon is re-mineralized into CO after entering the micro-food ring, except for most part through the respiration of microorganisms2And released into the atmosphere, a fraction (about 5%) of which can be converted by microorganisms into very stable inert DOC components that can be stored in the ocean for thousands of years.
The development of marine carbon sinks is one of the important ways to achieve the goal of "carbon neutralization". Although the importance of contributing inert DOC to water is recognized for large-sized algae, the carbon sink in the form of this part of inert DOC has not been included in the quantitative evaluation of algae carbon sink, mainly because the part of inert DOC is not deeply understood, and especially the identification of the stability component contained in the part of inert DOC is difficult.
Through the above analysis, the problems and defects of the prior art are as follows: although the importance of contributing inert DOC to water is recognized for large-sized algae, the carbon sink in the form of this part of inert DOC has not been included in the quantitative evaluation of algae carbon sink, mainly because the part of inert DOC is lack of deep understanding at present, and especially the identification of the stability component contained in the part of inert DOC is difficult.
The difficulty in solving the above problems and defects is:
at present, the identification of the macro algae derived DOC stable component is difficult, mainly because an accurate determination method of the inert DOC is not available internationally, and the inert DOC in the water body is not known deeply.
The significance of solving the problems and the defects is as follows:
aiming at the defects, the invention adopts a long-term microbial degradation experiment, combines the identification of FDOM fluorescent component and DOC molecular composition, analyzes the active, inert and stable components of the algae-derived DOC from two layers of qualitative and quantitative, and has important significance for scientifically evaluating the carbon sink function of the large-scale seaweed.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a method for identifying stable components in macroalgae-derived dissolved organic carbon and application thereof, and aims to evaluate and analyze inert DOC which can form long-term carbon sink in the ocean.
The invention is realized in such a way that the identification method of the stable components in the macroalgae source dissolved organic carbon comprises the following steps:
performing activity and inertia evaluation on large-scale seaweed DOC, analyzing the microbial availability of the seaweed DOC by utilizing a long-term microbial degradation experiment, and quantifying the inert DOC which is not finally utilized by microorganisms;
identifying the fluorescent components of the algae-derived DOC, and analyzing the fluorescent components with different stabilities in the algae-derived DOC according to the component change of the fluorescence dissolved organic matter FDOM;
and step three, identifying the molecular composition of the algae-derived DOC, and analyzing the stability components of the algae-derived DOC from the molecular level according to the molecular composition characteristics of the DOC.
Further, in the first step, the activity and inertness evaluation of the macroalgae-derived DOC comprises: the method comprises the steps of carrying out long-term microbial degradation experiments on the algae-derived DOC, and evaluating the algae-derived DOC with different stabilities according to the dynamic change characteristics of the DOC and by combining a second-order exponential decay model.
Furthermore, the inert DOC obtained by the microbial degradation experiment is only inert to microorganisms, so that a chemical and biological combination method is adopted to carry out photodegradation and repeated degradation of fresh microorganisms on the DOC which is not utilized by the microorganisms, and finally the DOC which is not utilized by the microorganisms can be regarded as stable DOC.
Further, in the first step, the activity and inertness evaluation of the macroalgae-derived DOC further comprises: model fitting is carried out on DOC concentration and degradation time by analyzing the dynamic change of DOC in long-time microbial degradation, and DOCs with different stability, namely activity, semi-activity and inertia, are judged according to a fitting equation.
Further, in the first step, the activity and inertness evaluation of the macroalgae-derived DOC further comprises:
DOC with different stabilities were evaluated by using a Marquardt nonlinear least squares fitting procedure: active DOC i.e. C1Semi-active DOC, i.e. C2Inert DOC, i.e. C3As shown in the following equation:
CT＝C1e-k1t+C2e-k2t+C3；
wherein, CTIs the total DOC concentration at time t, C1Characterization of active DOC size, C2Characterization of semi-active DOC size, C3Characterize inert DOC size, and k1And k2Is the decay constant of the active DOC and the semi-active DOC.
Further, in the second step, the identification of the fluorescent component of the algae-derived DOC comprises: main component analysis is carried out on fluorescence dissolved organic matter FDOM in long-term microbial degradation of algae-derived DOC, protein-like substances contained in the fluorescence dissolved organic matter FDOM have high activity and can be quickly utilized by microorganisms, and the fluorescence intensity is gradually reduced, so that the fluorescence dissolved organic matter FDOM can be used for characterizing DOC active components; the humic-like substances are not easily utilized by microorganisms, and the fluorescence intensity is gradually increased in degradation, so that the DOC stability component can be characterized.
Further, in step three, the molecular composition identification of the algae-derived DOC comprises: the DOC in the long-term microbial degradation of the algae-derived DOC is analyzed by an FT-ICR MS (Fourier transform ion cyclotron resonance mass spectrometry) technology, and is divided into four types of molecules containing CHO-, CHNO-, CHOS-and CHNOS-by analyzing and degrading DOC molecules obtained at different time, so that the molecules showing low H/C, high O/C, high-efficiency saturated double bond DBE and high aromaticity index AI have high inertization characteristics and are determined to be DOC stability molecules.
Further, the method for identifying the stable components in the macroalgae-derived dissolved organic carbon further comprises the following steps: the stability component evaluation of the large-scale seaweed source DOC can be realized by combining the activity and the inertia evaluation of the large-scale seaweed source DOC, the fluorescent component identification of the large-scale seaweed source DOC and the molecular composition identification of the large-scale seaweed source DOC, and finally, the qualitative and quantitative evaluation of the inertia DOC in the large-scale seaweed source DOC is realized.
The invention also aims to provide application of the identification method of the stable component in the macroalgae-derived dissolved organic carbon in the evaluation of the stable component of the macroalgae-derived DOC.
The invention also aims to provide application of the identification method of the stable components in the macroalgae-derived dissolved organic carbon in qualitative and quantitative evaluation of the inert DOC in the algae-derived DOC.
By combining all the technical schemes, the invention has the advantages and positive effects that: according to the method for identifying the stable component of the macroalgae source dissolved in the organic carbon, provided by the invention, the activity and inertia evaluation of the macroalgae source DOC, the fluorescent component identification of the algae source DOC and the molecular composition identification of the algae source DOC are combined, so that the stability component evaluation of the macroalgae source DOC can be realized, and the qualitative and quantitative evaluation of the inertia DOC in the algae source DOC can be finally realized.
According to the method, the stability and component identification are carried out on the macro-algae source DOC, so that the quality and the quantity of the inert DOC generated by the macro-algae can be determined and quantified, the technical bottleneck that the water carbon sink contributed to the algae is difficult to evaluate at present is broken through, and the method has important scientific significance for quantifying an ocean carbon trading system and promoting the development of the macro-algae carbon sink industry in the future.
According to the method, the activity and the inertia degree of the DOC of the macroalgae are evaluated, and the stability fluorescent component and the molecular composition contained in the DOC are identified, so that the quantitative and qualitative evaluation of the DOC contributing to the macroalgae is realized. The method has important application value for realizing the quantification of the carbon sink amount contributed by the seaweed and promoting the development of the carbon sink industry of the large seaweed in the future.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the embodiments of the present invention will be briefly described below, and it is obvious that the drawings described below are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a flow chart of a method for identifying a stable component in a macroalgae-derived dissolved organic carbon according to an embodiment of the present invention.
FIG. 2 is a time variation characteristic of DOC derived from chlorella in long-term degradation of microorganisms provided in example 3 of the present invention, and a second-order exponential decay model fitting is performed on DOC concentration and degradation time; wherein the DOC concentration is rapidly reduced (0-10 days) to obtain the active DOC, the DOC concentration is slowly reduced (10-100 days) to obtain the semi-active DOC, and the DOC concentration is extremely slowly reduced to obtain the stable DOC, namely the inert DOC in the later period (100 days and 400 days).
Fig. 3 is a graph showing the characteristics of the change in the DOC concentration in the fresh microbial re-degradation (a) and the photodegradation (b) of the remaining DOC after the long-term degradation by the microbes provided in example 3 of the present invention, and finally the DOC which is not degraded by the new microbes and the photodegradation, i.e., the stabilized DOC.
FIG. 4 is a graph showing the intensity change of the fluorescent component of FDOM during long-term degradation of microorganisms provided in example 3 of the present invention; wherein C1 and C2 are proteinoid components, and C3 and C4 are humoid components.
FIG. 5 is a time variation characteristic of DOC from brown algae source in the long-term degradation of microorganisms provided in example 4 of the present invention, and a second-order exponential decay model fitting is performed on DOC concentration and degradation time; wherein DOC concentration is rapidly reduced (0-10 days) to obtain active DOC, slowly reduced (10-80 days) to obtain semi-active DOC, and slowly reduced to obtain stable (inert DOC) in later period (80-360 days).
FIG. 6 is a graph showing the intensity change of the fluorescent component of FDOM during long-term degradation of microorganisms provided in example 4 of the present invention; wherein C1 and C2 are proteinoid components, and C3 and C4 are humoid components.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Aiming at the problems in the prior art, the invention provides a method for identifying a stable component in macroalgae-derived dissolved organic carbon, which is described in detail below with reference to the accompanying drawings.
As shown in fig. 1, the method for identifying stable components in macroalgae-derived organic carbon according to the embodiment of the present invention includes the following steps:
s101, evaluating the activity and inertia of the DOC of the macroalgae source;
s102, identifying the fluorescent components of the algae-derived DOC;
s103, identifying the molecular composition of the algae-derived DOC.
The technical solution of the present invention will be further described with reference to the following examples.
Example 1
The invention is realized by the following technical scheme:
in a first aspect, a method for assessing the activity and inertness of macroalgae-derived DOC is provided. The method comprises the steps of carrying out long-term microbial degradation experiments on the algae-derived DOC, and evaluating the algae-derived DOC with different stabilities according to the dynamic change characteristics of the DOC and by combining a second-order exponential decay model. Specifically, model fitting is mainly carried out on DOC concentration and degradation time by analyzing the dynamic change of DOC in long-time microbial degradation, and the sizes of DOCs (namely activity, semi-activity and inertia) with different stabilities are judged according to a fitting equation. Namely, DOC with different stabilities are evaluated mainly by using a Marquardt nonlinear least squares fitting procedure: active DOC (C)1) Semi-active DOC (C2) Inert DOC (C)3) Specifically, as shown in formula 1:
CT＝C1e-k1t+C2e-k2t+C3(formula 1)
Wherein C isTIs the total DOC concentration at time t, C1Characterization of active DOC size, C2Characterization of semi-active DOC size, C3Characterize inert DOC size, and k1And k2Is the decay constant of the active DOC and the semi-active DOC. For example, in example 3, the active, semi-active and inert DOC sizes, respectively, are obtained from the fitting results: 230, 230 and 396, with corresponding degradation times: 0-10, 10-100, 100-400 days (see FIG. 2).
In addition, the inert DOC obtained in the microbial degradation experiment is only inert to microorganisms, so that the DOC which is not utilized by the microorganisms is subjected to photodegradation and repeated degradation of fresh microorganisms again by adopting a chemical and biological combination method, and finally the DOC which is not utilized by the microorganisms can be regarded as stable DOC. For example, in example 3, the microbial inert DOC that was not ultimately utilized by the microbes was 406 μ M, whereas 4% and 6% of this was degraded by new microbes and light, respectively (see fig. 3), and the stable DOC that remained was 366 μ M.
In a second aspect of the invention, techniques for identifying the fluorescent components of algae-derived DOC are provided. Performing main component analysis on a Fluorescence Dissolved Organic Matter (FDOM) in the long-term microbial degradation of the algae-derived DOC, wherein a protein-like substance contained in the FDOM can be quickly utilized by microbes due to high activity, and the fluorescence intensity is gradually reduced, so that the FDOM can be used for representing DOC active components; the humoid is not easy to be utilized by microorganisms, and the fluorescence intensity is gradually increased in degradation, so that the DOC stability component can be represented. For example, in example 3, C1 and C2 were identified as protein-like components, and the fluorescence intensity of the protein-like components gradually decreased with increasing degradation time (see fig. 4 a, fig. 4 b), i.e. identified as DOC-active components, while the fluorescence intensity of the humoid gradually increased (see fig. 4C, fig. 4 d), could be identified as DOC-stable components.
In a third aspect of the invention, techniques for identifying the molecular composition of algae-derived DOC are provided. The DOC in the algae-derived DOC long-term microbial degradation is analyzed by an ultra-high resolution Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS), the DOC is divided into four types of molecules containing CHO-, CHNO-, CHOS-and CHNOS-by analyzing and degrading DOC molecules obtained at different time, and the molecules showing low H/C, high O/C, high-efficiency saturated Double Bond (DBE) and high Aromaticity Index (AI) have high inertization characteristics and are considered to be DOC stability molecules. For example, in example 3, comparing the DOC molecular composition at days 0 and 400, the DOC at day 400 exhibited significantly low H/C, high O/C, high DBE, and high AI index, and then the DOC molecule at day 400 could be identified as a DOC stability molecule (see table 1).
According to the method, the stability component evaluation of the large-scale seaweed source DOC can be realized by combining the three contents, and finally, the qualitative and quantitative evaluation of the inert DOC in the seaweed source DOC can be realized.
According to the method, the stability and component identification are carried out on the macro-algae source DOC, so that the quality and the quantity of the inert DOC generated by the macro-algae can be determined and quantified, the technical bottleneck that the water carbon sink contributing to the algae is difficult to evaluate at present is broken through, and the method has important scientific significance for quantifying the ocean carbon trading system and promoting the development of the macro-algae carbon sink industry in the future.
Example 2
One embodiment of the present invention is to provide a method for evaluating the activity and inertness of macroalgae-derived DOC. Mixing large algae source DOC with microorganism, and performing long-term microbial degradation experiment under dark condition for more than 1 year, wherein samples are taken at 0 th, 5 th, 10 th, 20 th, 30 th, 60 th, 90 th, 120 th, 150 th, 180 th, 210 th, 240 th, 270 th, 300 th and 360 th days of the experiment. According to the time dynamic change characteristics of the DOC and the combination of a second-order exponential decay model, the active DOC which is rapidly utilized by microorganisms in a short time is reduced slowly, the semi-active DOC which is also utilized is finally reduced slowly, and the inert DOC which is stably existed for a long time is obtained. In addition, after the long-term microbial degradation experiment is finished, a part of fresh seawater microbes are added into the rest DOC samples again, the DOC samples are continuously degraded for 60 days, and the DOC concentration change is monitored, so that whether the stability degree of the newly added microbes is consistent or not is verified for the remaining DOC components after the long-term microbial degradation. And putting the rest DOC into a quartz cup, continuously degrading for 10 days under natural light, verifying the stability degree of the rest DOC to light, and finally, stabilizing the DOC which is not degraded by new microorganisms and light.
In addition, to identify the stability component of DOC, FDOM and FT-ICR MS samples were taken in long-term microbial degradation experiments of DOC of algae origin. The method comprises the steps of firstly, carrying out principal component analysis on the FDOM by using a three-dimensional fluorescence spectrum technology, analyzing different fluorescence components, and identifying the stability of the components according to the fluorescence intensity change of the different components. The protein-like component has higher activity, and the strength of the protein-like component is gradually reduced under the action of microorganisms, so that the protein-like component can be identified as a DOC active component; the humoid is higher in inertia, and the intensity of the humoid is gradually increased, so that the humoid can be identified as a DOC stability component. On the other hand, the molecular composition information of DOC is analyzed by adopting an FT-ICR MS technology, firstly, a DOC sample is subjected to solid phase extraction, the sample subjected to solid phase extraction is diluted by adopting methanol, and is injected into a mass spectrometer for measurement through an Apollo II electrospray ion source, and finally, the molecular composition information of the algae source DOC can be obtained through FT-ICR MS correction, data acquisition and processing. According to the resolved molecular formula, the molecules with low H/C, high O/C, high DBE and high Aromaticity Index (AI) are generally considered to have higher inertization characteristics and can be identified as DOC stability molecules; while molecules with high H/C, low O/C, low DBE and low Aromaticity Index (AI) have higher activity and can be identified as DOC-active molecules.
Example 3: identification of stability component of chlorella-derived DOC
The large green alga-enteromorpha is taken as a research object, enteromorpha alga is cultured for 30 days under the laboratory condition, and the cultured seawater is filtered by a GF/F filter membrane of 0.7 mu m to obtain the DOC of the alga source. Offshore microorganisms are added into the DOC, and long-term (400 days) microbial degradation experiments of the DOC are carried out under dark conditions. The results show that the enteromorpha algae-derived DOC has obvious dynamic and component characteristic changes under the action of microorganisms. Wherein, DOC concentration presents the characteristics of first rising and then falling, and the later stage gradually stabilizes. According to the exponential decay equation, DOCs in different time periods are divided into active DOCs, semi-active DOCs and inert DOCs. Obtaining the sizes of active DOC, semi-active DOC and inert DOC according to the fitting result: 230, 230 and 396, with corresponding degradation times: 0-10, 10-100, 100-400 days (see FIG. 2). In addition, the decay constants of the active DOC and the semi-active DOC are 0.10 and 0.01, respectively. And, the generated microbial inert DOC (406 μ M) is continuously subjected to fresh microbial re-degradation and photodegradation, wherein 4% and 6% are respectively degraded by the new microbes and the light (see fig. 3), and the DOC which is not changed all the time in the final system is the stable DOC, and the final content is 366 μ M.
In long-term degradation, the microbial-driven DOC gradually shifts from an active component (proteoid) that is readily available to an inert component (humoid) that is extremely stable, according to the FDOM fluorescence intensity change (see fig. 4), the gradually decreasing proteoid, i.e., DOC active components (C1 and C2), and the gradually increasing humoid, i.e., DOC stability components (C3 and C4). Furthermore, according to the molecular composition of FT-ICR MS analysis, molecules exhibiting low H/C, high O/C, high DBE, high Aromaticity Index (AI) at the end of degradation (400 days) could be identified as DOC-stable molecules (see table 1).
TABLE 1 characterization of DOC molecular composition changes in the long-term degradation of DOC microorganisms of green algae origin
In Table 1, DOC active molecules having high H/C, low O/C, low DBE and low AI values, and DOC stabilizing molecules having low H/C, high O/C, high DBE and high AI values.
Example 4: stability component identification of brown algae derived DOC
Large brown algae-kelp is taken as a research object, kelp bodies are cultured in a laboratory for 30 days, and cultured seawater is filtered through a GF/F filter membrane of 0.7 mu m to obtain the algae source DOC. Offshore microorganisms are added into the DOC, and long-term (360 days) microbial degradation experiments of the DOC are carried out under dark conditions. The result shows that the DOC of the kelp is significantly degraded under the action of microorganisms, wherein the DOC concentration is characterized by rising firstly and then falling, and is gradually stabilized in the later period. According to the exponential decay equation, DOCs in different time periods are divided into active DOCs, semi-active DOCs and inert DOCs. Obtaining the sizes of active DOC, semi-active DOC and inert DOC according to the fitting result: 21, 20 and 58, respectively, with degradation times: the decay constants of the active DOC and the semi-active DOC are 0.12 and 0.01 respectively for 0-10 days, 10-80 days and 80-360 days (see figure 5). Further analysis of the FDOM composition and intensity changes (see fig. 6), progressively decreasing proteinoids, i.e. DOC active components (C1 and C2), and progressively increasing humoids, i.e. DOC stability components (C3 and C4). Furthermore, molecules that show low H/C, high O/C, high DBE, high Aromaticity Index (AI) at the end of degradation can be identified as DOC-stable molecules based on the molecular composition of the FT-ICR MS analysis, compared to the initial DOC (see table 2).
TABLE 2 characterization of DOC molecular composition changes in long-term degradation of DOC microorganisms from Phaeophyta algae
In Table 2, DOC active molecules having high H/C, low O/C, low DBE and low AI values, and DOC stabilizing molecules having low H/C, high O/C, high DBE and high AI values.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any modification, equivalent replacement, and improvement made by those skilled in the art within the technical scope of the present invention disclosed in the present invention should be covered within the scope of the present invention.
Claims (10)
1. A method for identifying a stable component in a macroalgae-derived dissolved organic carbon is characterized by comprising the following steps:
evaluating the activity and inertia of the macroalgae derived DOC;
identifying the fluorescent component of the algae-derived DOC;
and identifying the molecular composition of the algae-derived DOC.
2. The method of identifying a stable component of macroalgae-derived dissolved organic carbon of claim 1, wherein the macroalgae-derived DOC activity and inertness assessment comprises: the method comprises the steps of carrying out long-term microbial degradation experiments on the algae-derived DOC, and evaluating the algae-derived DOC with different stabilities according to the dynamic change characteristics of the DOC and by combining a second-order exponential decay model.
3. The method of claim 2, wherein the DOC obtained from the macro algae degradation experiment is only inert to microorganisms, so that the DOC not utilized by the microorganisms is subjected to the photodegradation and the fresh microorganisms are repeatedly degraded by a chemical and biological combination method, and finally the DOC not utilized by the microorganisms can be regarded as the stable DOC.
4. The method of identifying a stable component of macroalgae-derived dissolved organic carbon of claim 1, wherein the macroalgae-derived DOC is evaluated for activity and inertness, further comprising: model fitting is carried out on DOC concentration and degradation time by analyzing the dynamic change of DOC in long-time microbial degradation, and DOCs with different stability, namely activity, semi-activity and inertia, are judged according to a fitting equation.
5. The method of identifying a stable component of macroalgae-derived dissolved organic carbon of claim 1, wherein the macroalgae-derived DOC is evaluated for activity and inertness, further comprising: DOC with different stabilities were evaluated by using a Marquardt nonlinear least squares fitting procedure: active DOC i.e. C1Semi-active DOC, i.e. C2Inert DOC, i.e. C3：
CT＝C1e-k1t+C2e-k2t+C3；
Wherein, CTIs the total DOC concentration at time t, C1Characterization of active DOC size, C2Characterization of semi-active DOC size, C3Characterize inert DOC size, andk1and k2Is the decay constant of the active DOC and the semi-active DOC.
6. The method for identifying a stable component in macroalgae-derived dissolved organic carbon according to claim 1, wherein the identification of the fluorescent component of the algae-derived DOC comprises: main component analysis is carried out on fluorescence dissolved organic matter FDOM in long-term microbial degradation of algae-derived DOC, protein-like substances contained in the FDOM are rapidly utilized by microorganisms due to high activity, and the fluorescence intensity is gradually reduced, so that the FDOM can be used for representing DOC active components; the humoid is not easy to be utilized by microorganisms, and the fluorescence intensity is gradually increased in degradation, so that the DOC stability component can be represented.
7. The method of identifying a stable component of macroalgae derived dissolved organic carbon of claim 1, wherein the identification of the molecular composition of the algae derived DOC comprises: performing ultra-high resolution Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) analysis on DOC in the long-term microbial degradation of algae-derived DOC, analyzing and degrading DOC molecules obtained at different time, dividing the DOC into four types of molecules containing CHO-, CHNO-, CHOS-and CHNOS-, and determining that the DOC has the characteristics of high inertness and low H/C, high O/C, high-efficiency saturated double bond DBE and high aromaticity index AI, and is a DOC stability molecule.
8. The method of identifying a stable component in macroalgae-derived dissolved organic carbon according to claim 1, further comprising: the stability component evaluation of the large-scale seaweed source DOC can be realized by combining the activity and the inertia evaluation of the large-scale seaweed source DOC, the fluorescent component identification of the large-scale seaweed source DOC and the molecular composition identification of the large-scale seaweed source DOC, and finally, the qualitative and quantitative evaluation of the inertia DOC in the large-scale seaweed source DOC is realized.
9. Use of the method of identifying a stable component in macroalgae-derived dissolved organic carbon according to any one of claims 1 to 8 in the evaluation of the stable component of macroalgae-derived DOC.
10. Use of the method for identifying a stable component in macroalgae-derived dissolved organic carbon according to any one of claims 1 to 8 for qualitative and quantitative evaluation of an inert DOC in algae-derived DOC.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115684325A (en) * | 2022-10-17 | 2023-02-03 | 生态环境部华南环境科学研究所(生态环境部生态环境应急研究所) | Ultrahigh-resolution mass spectrometry method for dissolving organic carbon and application thereof |
| CN117495400A (en) * | 2023-09-27 | 2024-02-02 | 暨南大学 | A method and system for assessing carbon sinks in cultivated large seaweed ecosystems |
| CN118483201A (en) * | 2024-05-11 | 2024-08-13 | 河北农业大学 | A method for quantifying inert components in dissolved organic carbon of tidal shellfish and its application |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20130021020A (en) * | 2011-08-22 | 2013-03-05 | (주)백년기술 | Method for mesuring biochemical oxygen demand |
| CN105899221A (en) * | 2013-12-23 | 2016-08-24 | 医疗品牌研究有限公司 | Dermatological composition based on algae and olive leaf extracts |
-
2021
- 2021-03-30 CN CN202110340618.2A patent/CN113504205A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20130021020A (en) * | 2011-08-22 | 2013-03-05 | (주)백년기술 | Method for mesuring biochemical oxygen demand |
| CN105899221A (en) * | 2013-12-23 | 2016-08-24 | 医疗品牌研究有限公司 | Dermatological composition based on algae and olive leaf extracts |
Non-Patent Citations (6)
| Title |
|---|
| 崔世兰;江宪如;: "土壤中微生物及其环境效益的浅析", 环境科学与管理, no. 07, 15 July 2011 (2011-07-15) * |
| 张传伦, 孙军, 刘纪化, 等: ""海洋微型生物碳泵理论的发展与展望"", 《中国科学:地球科学》, vol. 49, no. 12, 20 December 2019 (2019-12-20), pages 1933 - 1944 * |
| 方华军;杨学明;张晓平;梁爱珍;申艳;: "耕作及水蚀影响下坡耕地土壤有机碳动态模拟", 土壤学报, no. 05, 30 October 2006 (2006-10-30) * |
| 曹军骥;占长林;: "黑碳在全球气候和环境系统中的作用及其在相关研究中的意义", 地球科学与环境学报, no. 02, 15 June 2011 (2011-06-15) * |
| 李强, 黄雅丹, 何若雪 等: ""岩溶水体惰性有机碳含量及其存在机理"", 《岩矿测试》, vol. 37, no. 5, 15 September 2008 (2008-09-15), pages 475 - 478 * |
| 杨继松;刘景双;于君宝;王金达;周旺明;秦胜金;: "草甸湿地土壤溶解有机碳淋溶动态及其影响因素", 应用生态学报, no. 01, 28 January 2006 (2006-01-28), pages 113 - 117 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115684325A (en) * | 2022-10-17 | 2023-02-03 | 生态环境部华南环境科学研究所(生态环境部生态环境应急研究所) | Ultrahigh-resolution mass spectrometry method for dissolving organic carbon and application thereof |
| CN117495400A (en) * | 2023-09-27 | 2024-02-02 | 暨南大学 | A method and system for assessing carbon sinks in cultivated large seaweed ecosystems |
| CN118483201A (en) * | 2024-05-11 | 2024-08-13 | 河北农业大学 | A method for quantifying inert components in dissolved organic carbon of tidal shellfish and its application |
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