CN113499271A

CN113499271A - Anti-allergy compound and preparation method and application thereof

Info

Publication number: CN113499271A
Application number: CN202110710438.9A
Authority: CN
Inventors: 黄建平
Original assignee: Guangzhou Zhuanyan Biotechnology Co ltd
Current assignee: Guangzhou Zhuanyan Biotechnology Co ltd
Priority date: 2021-06-25
Filing date: 2021-06-25
Publication date: 2021-10-15

Abstract

The invention discloses an anti-allergy compound, which comprises a phase A, a phase B and a phase C. The water, the propylene glycol, the sodium hyaluronate and the beta-glucan in the phase A play a role in water replenishing and repairing; the lysis product of the yeast fermentation product, the plant soothing component and the soothing and allergy-free compound in the phase B can play the effects of moisturizing, repairing skin barriers, resisting allergy, relieving itching and removing redness; the preservative added into the phase C is an ingredient which is irritating to the skin and has the effects of antisepsis and antibiosis. The anti-allergy ingredients in the conventional anti-allergy cosmetics are generally less in addition amount, but the effective ingredients of the invention are more in addition amount, so that the effect of relieving and resisting allergy is better. The A phase, the B phase and the C phase have synergistic effect, and have the obvious effects of relieving and sensitizing, soothing and relieving itching, dispelling red and subsiding swelling and repairing after sensitization. The preparation method of the anti-allergy composition has simple steps and mild reaction conditions, the phase A, the phase B and the phase C are sequentially added according to the solubility, the temperature of the phase B and the phase C is required to be controlled not to exceed 50 ℃ when the phase B and the phase C are added, and the influence of high temperature on the activity of raw materials is avoided.

Description

Anti-allergy compound and preparation method and application thereof

Technical Field

The invention relates to the technical field of anti-allergy cosmetics, and particularly relates to an anti-allergy compound and a preparation method thereof.

Background

Skin allergy is an immune-mediated skin allergy. The skin is used as a first defense line of a human immune system, is directly contacted with the environment and is easier to be allergic compared with other organs, 15-20% of people worldwide have reported to have skin allergy, and common skin allergy phenomena comprise pruritus, wheal, erythema, purpura, pimple and the like. Nowadays, the variety of skin care products for external use is increasingly diversified, and the incidence rate of skin allergy tends to rise.

Antihistamines are commonly used to treat skin allergies, which undergo three stages of iteration. First generation antihistamines: such as chlorpheniramine and diphenhydramine, has obvious sedative effect and adverse reaction to central nerves, most common are lethargy and power, prolonged reaction time and the like. After taking the medicines, the work of driving, operating precise instruments and the like should be avoided. In addition, the medicine also has the function of anticholinergic, can cause symptoms such as dry mouth, dry eyes, blurred vision, constipation and the like, and can also induce glaucoma, so that people with benign prostatic hyperplasia, glaucoma, low liver and kidney functions and old patients use the medicine with cautions. Second generation antihistamines: the common cetirizine, loratadine, mizolastine, ebastine and the like have few adverse reactions but almost have no obvious choline resistance and sedative effect. It has recently been found that astemizole (astemizole) and terfenadine (minoxidil) can cause rare, severe cardiotoxicity, leading to fatal cardiac arrhythmias; the above adverse effects are exacerbated when used in combination with isoconazole and erythromycin. The third generation of antihistamines: such as desloratadine, fexofenadine and the like, the adverse reaction is lighter, and no cardiotoxicity is caused when the drug is used with erythromycin and ketoconazole, but potential unknown risks also exist.

In recent years, the side effect of chemical drugs for treating allergy is increasingly prominent, so that the prevention and treatment of skin allergy and a new skin care product containing anti-allergy Chinese herbal medicines are combined to be a research hotspot. However, the skin care product has complex components and contains various chemical substances, such as essence, perfume, preservative, whitening component, surfactant and the like which are common skin allergens. The common plant anti-allergy components added in cosmetics, such as chamomile, liquorice, purslane, rhodiola rosea and the like, are mainly concentrated on skin repair and maintenance, and the addition amount of the anti-allergy components is small.

Disclosure of Invention

In order to overcome the defects of the prior art, the invention aims to provide an anti-allergy compound which does not need to add medicinal components, does not contain essence, spice and stimulating components, has mild components and plays a role in relieving and resisting allergy; the second purpose of the invention is to provide a preparation method of the anti-allergy compound, which has simple steps and is suitable for industrial mass production; the invention also aims to provide the application of the anti-allergy compound.

One of the purposes of the invention is realized by adopting the following technical scheme:

an anti-allergy compound comprises the following components in percentage by mass:

phase A: 15-30% of water, 30-50% of propylene glycol, 0.05-0.2% of sodium hyaluronate and 0.5-2% of beta-glucan;

phase B: 3-10% of a secondary cracking yeast fermentation product lysate, 5-15% of plant soothing components and 5-15% of soothing essence;

and C phase: 0.05-2% of a preservative; the sum of the raw materials of the phase A, the phase B and the phase C is 100 percent.

In the components, water is used as a solvent, propylene glycol is used as a wetting agent, sodium hyaluronate is used as a humectant, and beta-glucan can play a role in resisting inflammation, repairing, regulating immunity, preserving moisture, promoting collagen synthesis, improving skin density and resisting aging.

The lysate of the yeast fermentation product is derived from edible probiotics, namely bifidobacterium, and the metabolite, the cytoplasm component, the cell wall component and a plurality of organelle fragments are obtained by culturing and cracking somatic cells. Contains active ingredients such as various amino acids, lipid, polysaccharide, adenosine, vitamins, trace minerals, vitamin B group, etc. Is safe to human body temperature and easy to be absorbed and utilized by skin. The active substance obtained by the biotechnology can moisten and nourish the skin, help the skin to resist the damage of the external environment and pressure to the skin, simultaneously can promote various protein gene expressions related to the non-epidermal differentiation and the skin barrier function, and has the effects of wrinkle removal, repair, moisture retention and skin elasticity enhancement.

The skin-care product is a cosmetic plant formula functional raw material which combines the thoughts of 'holism and dialectics' of the traditional Chinese medicine, aims at relieving symptoms of sensitive skin and consolidating the barrier for a long time, and has the effects of repairing the damaged skin barrier, relieving itching and inflammation, removing redness and swelling, and calming and soothing the skin.

Further, the plant soothing component is one or more of a combination of gentian extract, centella asiatica extract and swertia japonica extract.

The compendium of materia Medica records: gentiana scabra Bunge is bitter, astringent, cold and nontoxic. It is mainly used for treating cold and heat between bones and pathogenic qi due to convulsion. Can be used for treating damp-heat jaundice, yin swelling, pudendal pruritus, leukorrhagia, middle-jiao strengthening, eczema, pruritus, conjunctival congestion, deafness, hypochondriac pain, bitter taste in mouth, and convulsion. Has analgesic and tranquilizing effects. The radix Gentianae extract has effects of relieving itching, clearing heat, relieving pain and removing red swelling.

Record of "national medicine record three": the whole herb of swertia pseudochinensis Franch is used for carbuncle swelling and sore, red swelling and pain, acute mastitis, lung heat cough, burn and scald, damp-heat icterohepatitis, diarrhea, chronic throat red swelling; the root is mainly used for treating toothache; the leaf is mainly used for treating traumatic injury. The swertia extract has effects of rapidly relieving itching, stimulating keratinocyte proliferation, and improving epidermis state.

The record of Shen nong Ben Cao Jing: centella asiatica is bitter and cold in flavor and mainly treats severe heat, malignant sores, carbuncle-abscess, red skin , and fever. Raw Sichuan millet. The main components of centella asiatica include asiaticoside, madecassoside, etc. The centella asiatica extract has the main effects of: antibacterial, anti-inflammatory, promoting the growth of damaged skin, increasing the hydration degree of stratum corneum, and improving the barrier ability of epidermis.

Still further, the sumizia is a composition of butylene glycol, an extract of astragalus membranaceus root, an extract of saposhnikovia divaricata root, an extract of marigold, and an extract of gastrodia elata root, an extract of albizia julibrissin.

Further, the preservative is chlorphenesin, and the mass percent of the chlorphenesin in the anti-allergy compound is 0.05-0.15%.

Still further, the preservative is 1, 2-ethylene glycol; the 1, 2-glycol accounts for 0.5-2% of the anti-allergy compound by mass. In a specific production, the preservative is a combination of chlorphenesin and 1, 2-glycol.

The second purpose of the invention is realized by adopting the following technical scheme:

a method for preparing an anti-allergy complex, comprising the steps of:

1) sequentially adding the raw materials of the phase A into a pot, and stirring until the raw materials are uniformly dissolved;

2) then adding the phase B and the phase C in sequence, and stirring uniformly;

3) stirring, sampling and inspecting to obtain the anti-allergic compound.

Further, in the step 1), the raw materials of the phase A are sequentially added into a pot, the temperature is raised to 75-85 ℃, the mixture is stirred at a constant temperature for 25-30 min, and the rotating speed is 30-40 r/min.

Further, in the step 2), adding the phase B and the phase C when the temperature is reduced to 40-50 ℃; the stirring speed is 20-30 r/min.

Further, in the step 3), stirring is continued when the temperature is reduced to 35-40 ℃, wherein the stirring speed is 15-20 r/min.

The third purpose of the invention is realized by adopting the following technical scheme:

use of an anti-allergy complex for the preparation of a facial cleanser, a cosmetologist, a cream, an essence, a mask or a make-up.

Compared with the prior art, the invention has the beneficial effects that:

(1) the anti-allergy compound is divided into a phase A, a phase B and a phase C; the water, the propylene glycol, the sodium hyaluronate and the beta-glucan in the phase A play a role in water replenishing and repairing; the lysis product of the yeast fermentation product, the plant soothing component and the soothing and allergy-free compound in the phase B can play the effects of moisturizing, repairing skin barriers, resisting allergy, relieving itching and removing redness; the preservative added into the phase C is an ingredient which is irritating to the skin and has the effects of antisepsis and antibiosis. The anti-allergy ingredients in the conventional anti-allergy cosmetics are generally added in an amount of 1-2%, while the B-phase ingredients are added in an amount of more than 3%, so that the effective ingredients are added in more amounts, and the effects of relieving and resisting allergy are better. The A phase, the B phase and the C phase have synergistic effect, and have the obvious effects of relieving and sensitizing, soothing and relieving itching, dispelling red and subsiding swelling and repairing after sensitization.

(2) The plant soothing component is preferably a composition of a gentian extract, a centella asiatica extract and a swertia japonica extract, can inhibit the early stage of inflammatory reaction, quickly block a histamine channel, and effectively prevent and inhibit the generation of inflammatory factors; but also can repair damaged skin, strengthen the barrier function of the skin and strengthen the health of the skin.

(3) The preservative selected by the invention is chlorphenesin and 1, 2-glycol, besides the antibacterial and antiseptic effects, chlorphenesin can also be used as an antigen-related immunosuppressant for inhibiting IgE-mediated histamine release in medicine, and has the effects of preventing the generation of inflammatory factors and inhibiting the circulation of the inflammatory factors. The 1, 2-glycol can be used as a humectant, has the effects of sterilizing and moisturizing, has good solubility, and does not produce negative effects on human health.

(3) The preparation method of the anti-allergy compound has simple steps and mild reaction conditions, the phase A, the phase B and the phase C are sequentially added according to the solubility, the temperature for adding the phase B and the phase C is required to be controlled to be not more than 50 ℃, and the influence of high temperature on the activity of the raw materials is avoided.

Detailed Description

The present invention is further described below with reference to specific embodiments, and it should be noted that, without conflict, any combination between the embodiments or technical features described below may form a new embodiment.

Example 1

A method for preparing an anti-allergy complex, comprising the steps of:

1) sequentially adding the raw materials of the phase A into a pot, heating to 85 ℃, keeping the constant temperature, and stirring for 30 minutes at the speed of 40 r/min;

2) standing, cooling to 50 deg.C, sequentially adding phase B and phase C, and stirring at 30 r/min;

3) cooling to 40 deg.C, stirring at 20r/min, sampling, inspecting, filtering, and collecting liquid to obtain antiallergic compound.

Example 2

A method for preparing an anti-allergy complex, comprising the steps of:

1) sequentially adding the raw materials of the phase A into a pot, heating to 75 ℃, keeping the constant temperature, and stirring for 20 minutes at the speed of 30 r/min;

2) standing, cooling to 40 deg.C, sequentially adding phase B and phase C, and stirring at 20 r/min;

3) cooling to 35 deg.C, stirring at a rotation speed of 15r/min, sampling, inspecting, filtering, and collecting liquid to obtain antiallergic compound.

Example 3

A method for preparing an anti-allergy complex, comprising the steps of:

1) sequentially adding the raw materials of the phase A into a pot, heating to 85 ℃, keeping the constant temperature, and stirring for 20 minutes at the speed of 30 r/min;

2) standing, cooling to 50 deg.C, sequentially adding phase B and phase C, and stirring at 20 r/min;

3) cooling to 40 deg.C, stirring at 25r/min, sampling, inspecting, filtering, and collecting liquid to obtain antiallergic compound.

Example 4

A method for preparing an anti-allergy complex, comprising the steps of:

Comparative example 1

Comparative example 1 differs from example 1 in that: adding water to make up to 100% without adding beta-glucan. The remaining components and preparation were the same as in example 1.

Comparative example 2

Comparative example 2 differs from example 1 in that: no split yeast fermentation lysate was added, and water was added to make up to 100%. The remaining components and preparation were the same as in example 1.

Comparative example 3

Comparative example 3 differs from example 1 in that: adding no plant relieving component, and adding water to make up to 100%. The remaining components and preparation were the same as in example 1.

Comparative example 4

Comparative example 4 differs from example 1 in that: adding water to make up to 100% without adding Shuyinjiao. The remaining components and preparation were the same as in example 1.

Safety test

Experimental samples: the anti-sensitivity complex of examples 1-4; blank control group: purified water of the same dosage;

purpose of the experiment: evaluating the potential possibility of the sample causing adverse reaction of human skin;

the method is based on: technical specifications for cosmetic safety (2015).

The experimental steps are as follows: the anti-sensitivity compounds of examples 1-4 and the blank control groups were randomly distributed on the curved side area of the forearm of the volunteers according to the test area, and clean spot test devices were selected. The amount of the sample is 0.020-0.025 g. Observing skin reactions for 30min, 24h and 48h after a tested object spot tester is removed, and recording observation results according to skin reaction grading standard of skin-enclosed type spot test in technical Specification for cosmetic safety (2015). The specific data are as follows:

TABLE 1 results of the human skin patch test of the anti-sensitivity composites of examples 1-4

As can be seen from Table 1, the number of subjects who experienced grade 1 adverse skin reactions was less than 5 subjects who experienced grade 1 adverse skin reactions, and no grade 2 or more adverse skin reactions were observed, and thus it was found that the anti-allergy compositions of examples 1 to 4 did not cause adverse reactions on human skin.

Antipruritic test

Grouping experiments: 90 rats were randomly divided into 9 groups of 8 administration groups and 1 blank control group (only equal amount of distilled water was administered), and the clinical dose of the adult was 1.2mg/kg per day and the equivalent dose of the mouse was 0.007mg/kg per body weight of the adult, as calculated from "equivalent dose ratio table between human and animal in terms of body surface area conversion").

The molding method comprises the following steps: mice were externally applied with the anti-allergy compound of examples 1-4 and comparative examples 1-4 at a dose of 0.0007mg/kg for each group, and the blank control group was given an equal amount of distilled water, and each group was externally applied with the corresponding drug once, 0.1 mL/foot, twice a day, for three consecutive days. The day of experiment, the shaved part of the rat was abraded with coarse sandpaper to a degree of about 1cm, which was determined to damage the epidermis, not the dermis and to have slight bleeding of the skin². Dripping 0.01% phosphorus on wound surface 10min after the last application for 1 time0.05mL of histamine dihydrochloride each, and increasing concentrations of 0.01%, 0.02%, 0.03% … … every 3min, each 0.05 mL. Observing whether the rat licks the right hind paw after returning within 3min after dripping the concentration each time, if the phenomenon of licking the right hind paw does not appear, continuing dripping the histamine phosphate solution with the next higher concentration until the phenomenon of licking the right hind paw appears, and taking the total amount of the histamine phosphate given at the moment as an itching valve. The total amount of histamine phosphate given to each group of rats when they returned to the licks was observed and recorded. The data are as follows.

TABLE 2 Histamine phosphate scratchiness threshold parameters for examples 1-4 and comparative examples 1-4

As can be seen from Table 2, compared with the blank control group, the examples 1 to 4 and the comparative examples 1 to 4 are very significantly increased, and p is less than 0.01, which shows that the examples 1 to 4 and the comparative examples 1 to 4 have the effect of relieving itching. The higher the scratchiness threshold of histamine phosphate, the better the anti-allergy effect. Example 1 is the best example, and the data of comparative examples 1-4 show that the main components influencing the itching relieving effect are good desensitization, plant soothing components, schizosaccharomyces cerevisiae fermentation product lysate and beta-glucan in sequence. The addition of the soothing component, the plant soothing component, the lysate of the yeast fermentation product of the schizosaccharomyces cerevisiae and the beta-glucan can be increased to further improve the scratchiness threshold of the histamine phosphate.

Evaluation of human body efficacy

Grouping experiments: 80 subjects are selected, the age of the subjects is between 20 and 40 years, the sex of the male and the female is not limited, and the subjects are divided into 8 groups on average. The subjects have no skin problem before the experiment, firstly, the histamine with the concentration of 0.1% is used for stimulating the inner side of the arm of the human body, when the stimulation part of the subject has red swelling and pruritus, the anti-allergy compositions of the examples 1-4 and the comparative examples 1-4 are respectively coated, and the time of skin reddening and itching relieving is observed. The data are as follows.

TABLE 3 evaluation of efficacy in humans of anti-allergy compositions of examples 1-4 and comparative examples 1-4

As can be seen from Table 3, the anti-allergic composition of example 1 has good effects of relieving itching and removing redness, and can control the swelling and removing redness within 10 minutes, and examples 1-4 can all relieve allergic symptoms within 15 minutes. The anti-allergy effect of comparative example 4 was the worst, indicating that good comfort is the main component affecting the anti-allergy effect. According to the red fading and itching relieving time, the size of the components influencing the anti-allergy effect can be obtained, and the components are the good-allergy, the plant soothing components, the lysate of the yeast schizosaccharomyces cerevisiae fermentation product and the beta-glucan in sequence.

The above embodiments are only preferred embodiments of the present invention, and the protection scope of the present invention is not limited thereby, and any insubstantial changes and substitutions made by those skilled in the art based on the present invention are within the protection scope of the present invention.

Claims

1. An anti-allergy compound is characterized by comprising the following components in percentage by mass:

2. The anti-allergy complex of claim 1, wherein the plant soothing component is one or a combination of two or more of gentian extract, centella asiatica extract, and swertia japonica extract.

3. The anti-allergy complex of claim 1, wherein the allergy relief is preferably a combination of butylene glycol, an extract of the root of Astragalus membranaceus, an extract of the root of Saposhnikovia divaricata, an extract of calendula officinalis, an extract of the root of Gastrodia elata.

4. The anti-allergy compound according to claim 1, wherein the preservative is chlorphenesin, and the mass percentage of the chlorphenesin in the anti-allergy compound is 0.05-0.15%.

5. The anti-sensitivity complex according to claim 1, wherein the preservative is 1, 2-ethanediol; the 1, 2-glycol accounts for 0.5-2% of the anti-allergy compound by mass.

6. The method for preparing an anti-sensitivity complex according to any one of claims 1 to 5, which comprises the following steps:

2) then adding the phase B and the phase C in sequence, and stirring uniformly;

3) stirring, sampling and inspecting to obtain the anti-allergic compound.

7. The method for preparing the anti-allergy compound according to claim 6, wherein in the step 1), the raw materials of the phase A are sequentially added into a pot, the temperature is raised to 75-85 ℃, the mixture is stirred at a constant temperature for 25-30 min, and the rotating speed is 30-40 r/min.

8. The method for preparing the anti-allergy compound according to claim 6, wherein in the step 2), the phase B and the phase C are added when the temperature is reduced to 40-50 ℃; the stirring speed is 20-30 r/min.

9. The method for preparing the anti-allergy compound according to claim 6, wherein in the step 3), the stirring is continued when the temperature is reduced to 35-40 ℃, and the stirring speed is 15-20 r/min.

10. Use of an anti-allergy complex according to any of claims 1 to 5 for the preparation of a facial cleanser, a cosmetologist, a cream, a serum, a mask or a make-up.