CN113455512A

CN113455512A - Nematicidal composition containing allyl isothiocyanate and fluensulfone and application thereof

Info

Publication number: CN113455512A
Application number: CN202110923298.3A
Authority: CN
Inventors: 朱怀铜; 汪云强; 梅洪玲; 葛敏; 吴文静; 徐俊采; 黄永灿
Original assignee: Anhui Huilong Group Yinshan Pharmaceutical Co ltd
Current assignee: Anhui Huilong Group Yinshan Pharmaceutical Co ltd
Priority date: 2021-08-11
Filing date: 2021-08-11
Publication date: 2021-10-01
Anticipated expiration: 2041-08-11
Also published as: CN113455512B

Abstract

The invention provides a nematicidal composition containing allyl isothiocyanate and fluensulfone, wherein the mass ratio of the allyl isothiocyanate to the fluensulfone can be 1: 20-20: 1. The composition can be used for preventing and treating nematodes and has a remarkable synergistic effect.

Description

Nematicidal composition containing allyl isothiocyanate and fluensulfone and application thereof

Technical Field

The invention provides a nematicidal composition containing allyl isothiocyanate and fluensulfone and application thereof.

Background

Allyl Isothiocyanate (AITC) is a food additive, and has recently been found to have excellent insecticidal, bactericidal, nematicidal, and herbicidal effects. Allyl isothiocyanate, registered by jordan agricultural corporation of the united states as a soil disinfectant, available under the tradename Dazitol, is used in soil treatment to effectively kill or inhibit nematodes, harmful insects, pathogens, and weeds in soil.

The fluensulfone has unique action mechanism, has different action mechanisms compared with organophosphorus and carbamate nematicide, the former has irreversible killing effect on nematodes, and the latter can only control temporarily. The agent has contact killing effect on nematodes, and has effects of reducing activity of nematodes after contact, further paralysis, stopping feeding after 1 hr exposure, decreasing infection ability, decreasing egg laying ability, decreasing hatching rate of eggs, and preventing hatched larvae from surviving for a long time, and finally killing irreversibly. The fluensulfone has good control effect on various plant parasitic nematodes and has unusual killing activity on seriously harmful root-knot nematodes. Researches show that the medicament can effectively prevent and control the attack of pests such as meloidogyne javanica, meloidogyne south/northern, nematodiasis, white potato nematodes, meloidogyne columbioides, pratylenchus maydis, meloidogyne arachidis and the like on plant roots. As a non-fumigant nematicide with systemic property, the nematicide can also be used for preventing and controlling the nematodes which harm agricultural and horticultural crops.

There is no report in the prior art relating to the use of a combination of the two.

Disclosure of Invention

The invention relates to a nematicidal composition containing allyl isothiocyanate and fluensulfone, which has the following specific technical scheme:

a nematicidal composition containing allyl isothiocyanate and fluensulfone is disclosed, wherein the mass ratio of the allyl isothiocyanate to the fluensulfone can be 1: 2020: 1. The preferred mass ratio of allyl isothiocyanate to fluensulfone can be 2: 1-1: 5.

The nematicidal composition further comprises agriculturally acceptable auxiliary materials. The percentage content of the active components is 1-60%, the preparation of the composition can be missible oil, microemulsion and aqueous emulsion.

The nematicidal composition is used for controlling nematodes. Preferably, the nematode is a root-knot nematode.

Compared with the prior art, the invention has the following beneficial effects:

when the ratio of allyl isothiocyanate to fluensulfone is 2: 1-1: 5, the co-toxicity coefficient of the nematicidal composition is more than 120, and the nematicidal composition has a synergistic effect; the 10% sulfosulfolane and 24% sulfolane missible oil prepared by the invention has better control effect on tomato root-knot nematode. Can effectively reduce the dosage of active ingredients and slow down the generation of drug resistance of nematodes.

Detailed Description

The invention will be better understood from the following examples. However, those skilled in the art will readily appreciate that the description of the embodiments is only for illustrating the present invention and should not be taken as limiting the invention as detailed in the claims.

Example 1 indoor toxicity determination of allyl isothiocyanate and fluensulfone combination on Meloidogyne incognita

Test subjects: and (3) collecting soil for later use, wherein the southern root-knot nematodes grow in the soil all the year round.

The test method comprises the following steps: the test compound medicament is prepared into 7 series of mass concentrations according to the test proportion, single-dose control and blank control are set simultaneously, and each treatment is repeated for 4 times. 500g of field soil is filled in a 2L dryer, 35mL of prepared liquid medicine is added into each dryer, the treatment time is 7 days, the cover is opened, the air is opened for 4 days, the number of nematodes treated by each dryer is measured by a centrifugal method, and the control effect is calculated.

The prevention and treatment effect calculation formula is as follows:

the inhibitory median concentration EC50 was calculated using the least squares method, and the cotoxicity coefficient (CTC) was calculated using the Sun cloud Pepper method.

The composition exhibits antagonism when CTC < 80, additive effect when CTC < 120, synergistic effect when CTC > 120.

Measured virulence index (ATI) ═ standard agent EC 50/test agent EC50 x 100

Theoretical virulence index (TTI) ═ A agent virulence index x percent of A in the mixture + B agent virulence index x percent of B in the mixture

Co-toxicity coefficient (CTC) ═ measured toxicity index (ATI) of the mixture/Theoretical Toxicity Index (TTI) of the mixture x 100

And (3) test results: as can be seen from the table 1, the compounded allyl isothiocyanate and fluensulfone have good synergy and additive effect on the meloidogyne incognita at the mass ratio of 20: 1-1: 20, and when the ratio of the allyl isothiocyanate to the fluensulfone is 2: 1-1: 5, the cotoxicity coefficient is more than 120, so that the synergistic effect is achieved.

Table 1 indoor toxicity test results of allyl isothiocyanate and fluensulfone compounded on Meloidogyne incognita

Example 2: 10% sulfobenzide-sulfofenchol emulsifiable concentrate

5% of allyl isothiocyanate, 5% of fluensulfone, 500# 8% of agricultural emulsion, 1601# 6% of agricultural emulsion, 6% of cyclohexanone and the balance of xylene to 100%. The active ingredients, the emulsifier and the auxiliary agent are sequentially added into a mixing kettle according to the proportion of the formula and are uniformly stirred to prepare the 10 percent sulfosulfobenzide emulsifiable solution.

Example 3: 24% sulfoisopropyl sulfochol emulsion

4% of allyl isothiocyanate, 20% of fluensulfone, 500# 12% of agricultural emulsion, 1601# 5% of agricultural emulsion, 4% of methanol, 8% of cyclohexanone and the balance of xylene to 100%. The active ingredients, the emulsifier and the auxiliary agent are sequentially added into a mixing kettle according to the proportion of the formula and are uniformly stirred to prepare the 24 percent sulfosulfobenzide emulsifiable solution.

Example 4: determination of field control effect of compound medicament on tomato root-knot nematode

Ploughing and preparing land before transplanting tomatoes, tightly covering with a plastic film, dissolving each medicament in water according to the test dose, diluting with clear water of 450 kg/mu, and irrigating. The treatment time is 7 days, the film is required to be uncovered and deflated for 4 days before transplanting, the area of each cell is 50 square meters, 4 cells are arranged for each treatment, and the cells treated by clear water are used as blank reference. Investigating the control effect of each treatment in the adult plant stage, counting the nematode condition of each treatment by uprooting classification, and calculating the control effect. The calculation method comprises the following steps:

the grading standards adopted by root-knot nematodes are:

0, the root has no root knot;

1, a small number of small root knots are arranged on the fibrous roots, and the number of the root knots is less than 5;

3, most root systems are clean, obvious visible root knots are formed on fibrous roots, and the number of the root knots is less than 10;

5, a few of main roots are infected, the number of root knots is less than 5, and the number of root knots on fibrous roots is more than 10;

7, a large number of root knots are arranged on the main root, and all fibrous roots are provided with root knots;

9, all the root systems have root knots, and the overground parts of the plants wilted.

And (3) test results: as can be seen from Table 2, the 10% sulfosulfolane and 24% sulfolane emulsifiable concentrates prepared by the invention have better control effect on tomato root-knot nematodes.

Table 2: determination result of field control effect of compound medicament on tomato root-knot nematode

Medicament	The effective component dosage	Control effect%
			10% sulfobenzide-sulfofenchol emulsifiable concentrate	2kg/bm2	95.45
24% sulfoisopropyl sulfochol emulsion	2kg/hm2	92.04
			480g/L fluensulfone missible oil	2kg/bm2	83.28
CK

The above description is only a preferred embodiment of the present invention, and it is obvious to those skilled in the art that modifications may be made without departing from the scope of the present invention, and such modifications are also within the scope of the present invention.

Claims

1. A nematicidal composition containing allyl isothiocyanate and fluensulfone is characterized in that the mass ratio of the allyl isothiocyanate to the fluensulfone can be 1: 20-20: 1.

2. The nematicidal composition of claim 1, wherein the mass ratio of allyl isothiocyanate to fluensulfone is from 2: 1 to 1: 5.

3. A nematicidal composition according to claim 1 or 2, further comprising an agriculturally acceptable adjuvant.

4. A nematicidal composition according to claim 3 wherein the percentage of active ingredient is 1 to 60%.

5. The nematicidal composition according to claim 4, wherein the formulation of the composition is selected from the group consisting of emulsifiable concentrates, microemulsions, and aqueous emulsions.

6. Use of a nematicidal composition according to any one of claims 1 to 5 to control nematodes.

7. Use according to claim 6, wherein the nematode is a root-knot nematode.