CN113439637B - A light and simplified seedling-raising substrate for jujube tillering seedlings and its preparation method - Google Patents

Abstract

The invention discloses a light simplified seedling substrate for tillering seedlings of date palm and a preparation method thereof, and the substrate comprises a reagent A, a reagent B and a reagent C, wherein the reagent A is 1/4MS salt solution containing carbendazim which is diluted 800-1200 times and azoxystrobin which is diluted 800-1200 times; the reagent B is a mixed solution containing 300-600mg/L of naphthalene acetic acid solution, 100-200mg/L of indolebutyric acid solution and 50-100mg/L of 2, 4-dichlorophenoxyacetic acid solution; and the volume ratio of the naphthalene acetic acid solution to the indolebutyric acid solution to the 2, 4-dichlorophenoxyacetic acid solution is 2:1:1.5; the reagent C is a naphthalene acetic acid solution with the concentration of 0.3-0.7 mg/L. The rooting medium also comprises a fixing matrix B and a rooting matrix C; wherein the rooting matrix C comprises 65-75% of decomposed coconut husk, 7-15% of perlite, 5-10% of organic fertilizer, 7-15% of vermiculite and 0.1-0.4% of slow release fertilizer by volume percentage. The invention adopts an external intervention method to carry out in vitro culture on the tillered seedlings of the date palm, promotes rooting culture, and has the functions of disease resistance and insect prevention, wound repair promotion, rooting acceleration, sufficient nutrient guarantee, survival rate improvement, easy loading and fixation and the like.

Description

A light and simplified seedling-raising substrate for jujube tillering seedlings and its preparation method

technical field

The invention relates to the field of plant biotechnology, and more specifically relates to a light and simplified seedling-raising substrate for jujube tillering seedlings and a preparation method thereof.

Background technique

The date palm tree (scientific name: Phoenix dactylifera L.) belongs to the genus of Palmaceae. It is native to the desert oasis of North Africa or the surrounding areas of the Persian Gulf in southwest Asia. It is one of the four earliest domesticated and cultivated fruit trees by humans. It is currently in my country Hainan, Fujian, Guangxi, Yunnan, Guangdong and other tropical and subtropical regions are planted. The date palm, the fruit of the date palm tree, is rich in nutritional value, with a sugar content as high as 55%-70%, a protein content of 3%, and a variety of amino acids. It is also one of the main grains of the Arabs.

Date palm is a dioecious perennial crop, and the traits of the seedlings are seriously separated, and the excellent traits of the female parent cannot be maintained. In order to ensure the planting yield and quality of date palm per unit area, traditional commercial cultivation mainly carries out asexual reproduction by cutting tiller seedlings of female plants. At present, the international market has a huge demand for commercial seedlings of date palms, but the mother plant of date palms can only produce 15-30 mature tillering seedlings in a lifetime, and the tillering seedlings that have grown on the mother plant for 3-5 years can be removed for in vitro propagation. The number of tillering seedlings is limited, and the survival rate of cultivation is not high, thus limiting the rapid propagation of excellent seedlings.

In foreign countries, two methods are generally used to cultivate tillering seedlings. A kind of method is after the tillering seedling grows mature on the parent body, directly cuts down and moves in the nursery and carries out earth cultivation. Another method is to take different operations on the tillering seedlings growing in different parts of the mother body in order to promote rooting. One is to cover the base of the tillering seedlings with moist soil to promote rooting 12 months before removal of the tillering seedlings growing at the base of the mother body; The second is the tillering seedlings that grow at a higher position in the trunk of the parent body. Before removal, 12 months before removal, plastic bags or burlap with substrates are used to fix and moisturize the base to promote rooting. Therefore, there are the following problems in the existing tillering seedling cultivation technology: 1. the tillering seedlings taken off from the mother body are susceptible to infestation by diseases and insect pests, and the survival rate is not high; .A light and simple seedling-growing substrate that promotes roots without targeting, moisturizes and preserves fat.

Therefore, how to improve the survival rate of tillering seedling cultivation and accelerate the seedling raising speed under the condition of limited number of tillering seedlings is an urgent problem to be solved by those skilled in the art.

Contents of the invention

In view of this, the purpose of the present invention is to provide a kind of date palm tillering seedling light and simplified seedling-raising substrate and preparation method, by adopting the method for external intervention to carry out in vitro culture to date palm tillering seedling, promote rooting culture, have disease resistance and pest control, promote Wound repair, accelerate rooting, ensure sufficient nutrients, improve survival rate, easy shipment and planting and other functions.

In order to achieve the above object, the present invention adopts the following technical solutions:

A light and simplified seedling-raising substrate for jujube tillering seedlings, including reagent A, reagent B and reagent C;

The reagent A is a 1/4MS salt solution containing 800-1200-fold diluted carbendazim and 800-1200-fold diluted azoxystrobin; the reagent B is a naphthaleneacetic acid solution containing 300-600mg/L, A mixed solution of 200 mg/L indole butyric acid solution and 50-100 mg/L 2,4-dichlorophenoxyacetic acid solution; and the naphthalene acetic acid solution, indole butyric acid solution and 2,4-dichlorobenzene The volume ratio of the oxyacetic acid solution is 2:1:1.5; the reagent C is a 0.3-0.7 mg/L naphthaleneacetic acid solution.

Further a kind of date palm tillering seedling light simplified seedling-raising matrix, also comprises fixed matrix B and rooting matrix C; Wherein, described fixed matrix B is the matrix that has the effect of supporting date palm tillering seedling, is preferably the mixture of vermiculite and quartz sand, and The volume ratio of vermiculite and quartz sand is (80-90): (10-20); the rooting matrix C includes 65%-75% of decomposed coconut peat, 7%-15% of perlite, organic fertilizer 5%-10%, vermiculite 7%-15% and slow-release fertilizer 0.1%-0.4%.

The beneficial effects of the present invention are:

In reagent A, 1/4MS salt solution is used as a nutrient buffer to prevent the tillering seedlings from losing their nutrient supply instantly after leaving the mother body; Strostrobin is mainly used to prevent and kill infectious diseases caused by fungi in date palms. The combination of carbendazim and azoxystrobin in the present invention has a synergistic effect on the treatment of tiller base wounds, and can prevent germs from disseminating roots of tiller seedlings, accelerate wound repair, and improve the survival rate of tiller seedlings.

In reagent B, naphthaleneacetic acid (NAA) has the effect of inducing root germination and promoting rooting; Role in wound repair and parenchyma formation. In the present invention, three high-concentration hormones, naphthalene acetic acid, indole butyric acid and 2,4-dichlorophenoxyacetic acid, are quickly dipped in to stimulate the wound to produce callus, which can promote the rapid rooting of tillering seedlings and shorten the delay time of tillering seedlings. The effect of seedling cycle.

The combined use of decomposed coconut peat, perlite and vermiculite in the rooting medium C can provide an external environment for rapid root growth, help tillering seedlings absorb the required nutrients evenly, and maintain root stability under the long-term and continuous action of low-concentration naphthaleneacetic acid , Healthy growth. Organic fertilizers and slow-release fertilizers are used together to provide a stable and continuous supply of nutrients at the seedling stage. The light and simple rooting matrix has the functions of moisturizing and maintaining fertilizer, and accelerates the penetration of the root system, so as to achieve the effect of strong seedlings, and is convenient for shipment and transplantation.

Further, the reagent A is a 1/4MS salt solution containing 1000-fold diluted carbendazim and 800-fold diluted azoxystrobin; the reagent B is a solution containing 400 mg/L of naphthalene acetic acid and 150 mg/L of indole A mixed solution of butyric acid solution and 80mg/L 2,4-dichlorophenoxyacetic acid solution; the reagent C is a 0.5mg/L naphthaleneacetic acid solution.

Further, the rooting matrix C includes 70% of decomposed coconut peat, 10% of perlite, 8% of organic fertilizer, 11.8% of vermiculite and 0.2% of slow-release fertilizer in volume percentage.

Furthermore, the slow-release fertilizer has a fertilizer effect of more than 180 days, which is (15-9-11+2MgO+TE).

Another object of the present invention is to provide a method for preparing the light and simplified seedling-raising substrate of the above-mentioned date palm tillering seedlings, which specifically includes the following steps:

(1) Preparation of reagent A: Prepare 1/4MS saline solution with sterile water, then weigh carbendazim and azoxystrobin of corresponding dilution multiples, dissolve in 1/4MS saline solution, and obtain reagent A;

(2) Preparation of reagent B: weigh naphthaleneacetic acid, indolebutyric acid and 2,4-dichlorophenoxyacetic acid, dissolve them completely with sodium hydroxide solution, adjust the pH value to 5.5-6.0, add sterile water to to the corresponding concentration, and then mixed according to the volume ratio of naphthaleneacetic acid solution, indolebutyric acid solution and 2,4-dichlorophenoxyacetic acid solution in a ratio of 2:1:1.5 to obtain reagent B;

(3) Preparation of reagent C: weigh naphthaleneacetic acid, dissolve it completely with sodium hydroxide solution, adjust the pH value to 5.5-6.0, add sterile water to the corresponding concentration, and obtain reagent C.

Further, in steps (2) and (3), the molar concentration of the sodium hydroxide solution is 1mol/L.

Further, the preparation method of jujube tillering seedling lightly simplified seedling-raising substrate also includes:

(4) Prepare the fixation matrix B: sterilize the fixation matrix B, soak it with sterile water for later use;

Preferably wrapping the fixed matrix B with kraft paper and performing high temperature and high pressure moist heat sterilization;

(5) Preparation of rooting substrate C: Mix sterilized decomposed coconut peat, perlite, organic fertilizer, vermiculite and slow-release fertilizer in proportion, and set aside.

Beneficial effects: the present invention uses sodium hydroxide solution to dissolve growth hormones such as naphthaleneacetic acid, and analytically pure naphthaleneacetic acid, indolebutyric acid, and 2,4-dichlorophenoxyacetic acid are in powder form, and methanol or ethanol is usually used to dissolve them In order to prepare the required solution concentration, but the inventor found in long-term experiments that the use of methanol or ethanol dissolved naphthalene acetic acid, indole butyric acid and 2,4-dichlorophenoxyacetic acid, etc. are prone to precipitation after being placed. Snowflake-like substances are precipitated in the solution, but the properties of the solution after dissolving with sodium hydroxide are stable, and there is no drug precipitation during the storage period.

Another object of the present invention is to provide the method for using the above-mentioned jujube tillering seedling light and simplified seedling raising substrate, which specifically includes the following steps:

(1) pre-emergence treatment of date palm tillering;

(2) Remove the date palm tillering seedling from the parent body, soak the base of the tillering seedling in reagent A, take it out after 15-20 min, and rinse it with sterile water to obtain the date palm tillering seedling I;

(3) Soak the base of jujube tillering seedling I in reagent B, take it out after 40-50 minutes, and then plant it in fixed substrate B, plant one plant in each pot, spray sterile water every day to keep the environment of the tillering seedling base moist, and cultivate Take it out after 30-40 days to obtain date palm tillering seedling II;

(4) Add reagent C to rooting matrix C, and the volume ratio is rooting matrix C:reagent C=2:1, then plant date palm tillering seedling II, plant one plant in each pot, and water 1L of reagent every 25-35 days C, continuous watering 3-5 times, during this period, spray sterile water to keep the moistening of matrix C, obtain the jujube tillering seedling with solid root;

(5) Strong seedlings, transplanted.

Further, in the above-mentioned step (1), the specific operation of the pre-treatment of date palm tiller seedlings is: before the date palm tiller seedlings are taken off, remove dead branches and base impurities of date palm tiller seedlings, and use 3% thiacloprid microcapsules The 1500-2000 times dilution of the suspension agent and the 1200-1500 times dilution of 20% propiconazole EC are mixed to spray the whole plant of the date palm tillering seedlings, spray again after an interval of 14 days, and remove the date palm tillering seedlings from the mother after 7 days.

Beneficial effects: before the tillering seedlings are separated from the parent body, the dead branches and base impurities of the tillering seedlings are removed to expose the base of the tillering seedlings, and then sprayed with insecticides and fungicides to reduce the insect eggs and germs carried by the tillering seedlings, It can reduce the chance of tillering seedlings being infested.

Via the above-mentioned technical scheme, it can be seen that compared with the prior art, the beneficial effects of the present invention are as follows:

1. In the present invention, carbendazim and azoxystrobin are used in combination, which has a synergistic effect on the treatment of tiller base wounds, and can prevent the infection of the roots of tiller seedlings by pathogens, speed up the repair of incisions, and improve the survival rate of tiller seedlings.

2. In the present invention, three high-concentration hormones, naphthalene acetic acid, indole butyric acid and 2,4-dichlorophenoxyacetic acid, are subjected to rapid dipping treatment, which can achieve the effects of promoting rapid rooting of tillering seedlings and shortening the slowing down cycle of tillering seedlings.

3. The present invention combines low-concentration hormones, nutrient substrates and rooting substrates, which can not only ensure the robust growth of tillering seedlings, but also promote the development of the root system of tillering seedlings; the nutrient substrate and rooting substrate have the advantages of better permeability, moisture retention and fat preservation. , to speed up root penetration and nutrient absorption, shorten the period of strong seedlings; and the weight of the nutrient matrix and rooting matrix is light, which is conducive to the shipment and transplanting of tillering seedlings in the later stage, and saves labor costs.

Detailed ways

The following clearly and completely describes the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

Example 1

Jujube tillering seedlings are light and simplified seedling substrates, including reagent A, reagent B and reagent C; wherein, reagent A is 1/4MS salt solution containing 800-fold diluted carbendazim and 1200-fold diluted azoxystrobin; reagent B is A mixed solution containing 300 mg/L naphthalene acetic acid solution, 200 mg/L indole butyric acid solution and 100 mg/L 2,4-dichlorophenoxyacetic acid solution; and naphthalene acetic acid solution, indole butyric acid solution and 2, The volume ratio of 4-dichlorophenoxyacetic acid solution is 2:1:1.5; reagent C is 0.3mg/L naphthaleneacetic acid solution.

The date palm tillering seedling light simplified seedling-raising matrix also includes fixed matrix B and rooting matrix C; Wherein, fixed matrix B comprises vermiculite 80% and quartz sand 20% by volume percentage; Rooting matrix C comprises volume percentage Decomposed coconut peat 65%, perlite 15%, organic fertilizer 5%, vermiculite 14.9% and slow release fertilizer 0.1%. The composition of the slow-release fertilizer is (15-9-11+2MgO+TE).

The preparation method of the present embodiment jujube tillering seedling raising substrate specifically comprises the following steps:

(1) Preparation of reagent A: Prepare 1/4MS saline solution with sterile water, then weigh carbendazim and azoxystrobin of corresponding dilution multiples, dissolve in 1/4MS saline solution, and obtain reagent A;

(2) Preparation of reagent B: Weigh naphthaleneacetic acid, indolebutyric acid and 2,4-dichlorophenoxyacetic acid, respectively dissolve them completely with 1mol/L sodium hydroxide solution, adjust the pH value to 5.5-6.0, add The bacterial water was adjusted to the corresponding concentration, and then mixed according to the volume ratio of naphthalene acetic acid solution, indole butyric acid solution and 2,4-dichlorophenoxyacetic acid solution at a ratio of 2:1:1.5 to obtain reagent B;

(3) Preparation of reagent C: weigh naphthaleneacetic acid, dissolve it completely with 1mol/L sodium hydroxide solution, adjust the pH value to 5.5-6.0, add sterile water to the corresponding concentration, and obtain reagent C.

(4) Preparation of fixed substrate B: mix vermiculite and quartz sand, wrap it in kraft paper and sterilize it at high temperature and high temperature, take it out and soak it with sterile water for later use;

(5) Preparation of rooting substrate C: Mix sterilized decomposed coconut peat, perlite, organic fertilizer, vermiculite and slow-release fertilizer in proportion, and set aside.

The using method of the above-mentioned jujube tillering seedling raising substrate of the present embodiment specifically comprises the following steps:

(1) date palm tillering seedlings pretreatment, remove dead branches and base impurities of date palm tillering seedlings, and use 1500 times of dilutions of 3% thiacloprid microcapsule suspension and 1500 times of dilutions of 20% propiconazole emulsifiable concentrate to mix The whole plant of date palm tillering seedlings is sprayed, and after an interval of 14 days, it is sprayed again, and after 7 days, the date palm tillering seedlings are taken off from the parent body;

(2) Remove the date palm tillering seedlings from the parent body, soak the base of the tillering seedlings in reagent A, take them out after 15 minutes, and rinse them with sterile water to obtain date palm tillering seedlings I;

(3) Soak the base of date palm tillering seedling I in reagent B, take it out after 40 minutes, and then plant it in the fixed substrate B, plant one plant in each pot, spray sterile water to keep the environment moist, and check the rooting situation at the wound regularly , taken out after 30 days of cultivation to obtain date palm tillering seedling II;

(4) Add reagent C to the rooting matrix C, and the volume ratio is rooting matrix C:reagent C=2:1, then plant date palm tillering seedling II, plant one plant in each pot, and water 1L of reagent C every 25 days, Irrigate continuously 5 times, during this period, spray sterile water to keep the moistening of matrix C, obtain the jujube tillering seedling with solid root;

(5) Strong seedlings, transplanted.

Example 2

Jujube tillering seedlings are light and simplified seedling substrates, including reagent A, reagent B and reagent C; wherein, reagent A is 1/4MS salt solution containing 1000-fold diluted carbendazim and 800-fold diluted azoxystrobin; reagent B is A mixed solution containing 400 mg/L naphthalene acetic acid solution, 150 mg/L indole butyric acid solution and 80 mg/L 2,4-dichlorophenoxyacetic acid solution; and naphthalene acetic acid solution, indole butyric acid solution and 2, The volume ratio of 4-dichlorophenoxyacetic acid solution is 2:1:1.5; reagent C is 0.5mg/L naphthaleneacetic acid solution.

The date palm tillering seedling light simplified seedling-raising matrix also includes fixed matrix B and rooting matrix C; Wherein, fixed matrix B includes vermiculite 85% and quartz sand 15% in volume percentage; Rooting matrix C includes in volume percentage Decomposed coconut peat 70%, perlite 10%, organic fertilizer 8%, vermiculite 11.8% and slow release fertilizer 0.2%.

The preparation method of the present embodiment jujube tillering seedling raising substrate specifically comprises the following steps:

(1) Preparation of reagent A: Prepare 1/4MS saline solution with sterile water, then weigh carbendazim and azoxystrobin of corresponding dilution multiples, dissolve in 1/4MS saline solution, and obtain reagent A;

(2) Preparation of reagent B: Weigh naphthaleneacetic acid, indolebutyric acid and 2,4-dichlorophenoxyacetic acid, respectively dissolve them completely with 1mol/L sodium hydroxide solution, adjust the pH value to 5.5-6.0, add The bacterial water was adjusted to the corresponding concentration, and then mixed according to the volume ratio of naphthalene acetic acid solution, indole butyric acid solution and 2,4-dichlorophenoxyacetic acid solution at a ratio of 2:1:1.5 to obtain reagent B;

(3) Preparation of reagent C: weigh naphthaleneacetic acid, dissolve it completely with 1mol/L sodium hydroxide solution, adjust the pH value to 5.5-6.0, add sterile water to the corresponding concentration, and obtain reagent C.

(4) Preparation of fixed substrate B: mix vermiculite and quartz sand, wrap it in kraft paper and sterilize it at high temperature and high temperature, take it out and soak it with sterile water for later use;

(5) Preparation of rooting substrate C: Mix sterilized decomposed coconut peat, perlite, organic fertilizer, vermiculite and slow-release fertilizer in proportion, and set aside.

The using method of the above-mentioned jujube tillering seedling raising substrate of the present embodiment specifically comprises the following steps:

(1) date palm tillering seedlings pretreatment, remove dead branches and base impurities of date palm tillering seedlings, and use 1800 times of dilutions of 3% thiacloprid microcapsule suspension and 1300 times of dilutions of 20% propiconazole emulsifiable concentrate to mix The whole plant of date palm tillering seedlings is sprayed, and after an interval of 14 days, it is sprayed again, and after 7 days, the date palm tillering seedlings are taken off from the parent body;

(2) Remove the date palm tillering seedlings from the mother body, soak the base of the tillering seedlings in reagent A, take them out after 18 hours, and rinse them with sterile water to obtain date palm tillering seedlings I;

(3) Soak the base of date palm tillering seedling I in reagent B, take it out after 45 minutes, and then plant it in the fixed substrate B, plant one plant in each pot, spray sterile water every day to keep the environment moist, and check the rooting of the wound regularly situation, after 35 days of cultivation, it was taken out to obtain date palm tillering seedling II;

(4) Add reagent C to rooting matrix C, and the volume ratio is rooting matrix C:reagent C=2:1, then plant date palm tillering seedling II, plant one plant in each pot, and water 1L of reagent C quantitatively every 30 days , continuous watering 4 times, during this period, spray sterile water to keep the moistening of matrix C, obtain the date palm tillering seedlings with solid roots;

(5) Strong seedlings, transplanted.

Example 3

Jujube tillering seedlings are light and simplified seedling substrates, including reagent A, reagent B and reagent C; wherein, reagent A is 1/4MS salt solution containing 1200-fold diluted carbendazim and 1000-fold diluted azoxystrobin; reagent B is A mixed solution containing 600 mg/L naphthalene acetic acid solution, 100 mg/L indole butyric acid solution and 50 mg/L 2,4-dichlorophenoxyacetic acid solution; and naphthalene acetic acid solution, indole butyric acid solution and 2, The volume ratio of 4-dichlorophenoxyacetic acid solution is 2:1:1.5; reagent C is 0.7mg/L naphthaleneacetic acid solution.

The date palm tillering seedling light simplified seedling-raising matrix also includes fixed matrix B and rooting matrix C; Wherein, fixed matrix B includes vermiculite 90% and quartz sand 10% in volume percentage; Rooting matrix C includes in volume percentage Decomposed coconut peat 75%, perlite 7%, organic fertilizer 10%, vermiculite 7.6% and slow release fertilizer 0.4%.

The preparation method of the present embodiment jujube tillering seedling raising substrate specifically comprises the following steps:

(1) Preparation of reagent A: Prepare 1/4MS saline solution with sterile water, then weigh carbendazim and azoxystrobin of corresponding dilution multiples, dissolve in 1/4MS saline solution, and obtain reagent A;

(2) Preparation of reagent B: Weigh naphthaleneacetic acid, indolebutyric acid and 2,4-dichlorophenoxyacetic acid, respectively dissolve them completely with 1mol/L sodium hydroxide solution, adjust the pH value to 5.5-6.0, add The bacterial water was adjusted to the corresponding concentration, and then mixed according to the volume ratio of naphthalene acetic acid solution, indole butyric acid solution and 2,4-dichlorophenoxyacetic acid solution at a ratio of 2:1:1.5 to obtain reagent B;

(3) Preparation of reagent C: weigh naphthaleneacetic acid, dissolve it completely with 1mol/L sodium hydroxide solution, adjust the pH value to 5.5-6.0, add sterile water to the corresponding concentration, and obtain reagent C.

(4) Preparation of fixed substrate B: mix vermiculite and quartz sand, wrap it in kraft paper and sterilize it at high temperature and high temperature, take it out and soak it with sterile water for later use;

(5) Preparation of rooting substrate C: Mix sterilized decomposed coconut peat, perlite, organic fertilizer, vermiculite and slow-release fertilizer in proportion, and set aside.

The using method of the above-mentioned jujube tillering seedling raising substrate of the present embodiment specifically comprises the following steps:

(1) date palm tillering seedlings pretreatment, remove dead branches and base impurities of date palm tillering seedlings, and use 2000 times of dilutions of 3% thiacloprid microcapsule suspension and 1200 times of dilutions of 20% propiconazole emulsifiable concentrate to mix The whole plant of date palm tillering seedlings is sprayed, and after an interval of 14 days, it is sprayed again, and after 7 days, the date palm tillering seedlings are taken off from the parent body;

(2) Remove the date palm tillering seedlings from the parent body, soak the base of the tillering seedlings in reagent A, take them out after 20 minutes, and rinse them with sterile water to obtain date palm tillering seedlings I;

(3) Soak the base of date palm tillering seedling I in reagent B, take it out after 50 minutes, and then plant it in the fixed substrate B, plant one plant in each pot, spray sterile water every day to keep the environment moist, and check the rooting of the wound regularly situation, after 40 days of cultivation, it was taken out to obtain date palm tillering seedling II;

(4) Add reagent C to the rooting matrix C, and the volume ratio is rooting matrix C:reagent C=2:1, then plant date palm tillering seedling II, plant one plant in each pot, and water 1L of reagent C every 35 days, Irrigate continuously 3 times, during this period, spray sterile water to keep the moistening of matrix C, obtain the jujube tillering seedling with solid root;

(5) Strong seedlings, transplanted.

Comparative example 1

The difference between the jujube tillering seedling raising substrate and that of Example 2 is that it does not contain reagent A.

Comparative example 2

The seedling raising substrate of date palm tillering seedlings only differs from Example 2 in that it does not contain fixed substrate B and reagent B.

Comparative example 3

Date palm tillering seedling seedling raising matrix, the difference with embodiment 2 is only, does not contain matrix C and reagent C, after finishing the 1-3 step in the using method in the above-mentioned date palm tillering seedling seedling raising matrix of embodiment 2, directly pack into sandy loam Seedlings were grown in culture bags.

Comparative example 4

No pretreatment is performed, only reagent A is used to treat the jujube tillering seedlings, and the seedlings are directly put into sandy loam culture bags for seedling cultivation.

Performance Testing

Statistical embodiment 1-3 and comparative example 1-4 date palm tillering seedling root number before strong seedling, strong seedling period and final survival rate.

From the removal of the tiller seedlings to the treatment and seedling raising time reaching 150 days, the number of first-order roots and second-order roots was uniformly recorded, and the cycle of strong seedlings and survival rate were counted after 365 days. The statistical results are shown in Table 1.

Table 1 embodiment 1-3 and comparative example 1-4 nursery statistical result

As can be seen from Table 1, compared with Comparative Example 4, the first-order roots, second-order root quantity and strong seedling period of the date palm tillering seedlings treated with the substrate of Comparative Example 1-3 date tillering seedlings have been improved to a certain extent.

Compared with comparative example 4, the first-order root, secondary root quantity and strong seedling cycle of the date palm tillering seedling after the substrate treatment of embodiment 1-3 date tillering seedling raising are extremely significantly improved, and are obviously higher than comparative example 1- 3. The invention realizes the effects of improving the seedling growth period of jujube tillering seedlings and accelerating the seedling raising speed.

The above description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the present invention will not be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims (8)

1. The light simplified seedling substrate for the tillered seedlings of the date palm is characterized by comprising a reagent A, a reagent B and a reagent C;

the reagent A is 1/4MS salt solution containing 800-1200 times diluted carbendazim and 800-1200 times diluted azoxystrobin;

the reagent B is a mixed solution containing 300-600mg/L of naphthalene acetic acid solution, 100-200mg/L of indolebutyric acid solution and 50-100mg/L of 2, 4-dichlorophenoxyacetic acid solution; and the volume ratio of the naphthalene acetic acid solution to the indolebutyric acid solution to the 2, 4-dichlorophenoxyacetic acid solution is 2:1:1.5;

the reagent C is 0.3-0.7mg/L of naphthalene acetic acid solution;

the rooting medium also comprises a fixing matrix B and a rooting matrix C; the fixing matrix B is a mixture of vermiculite and quartz sand, and the volume ratio of the vermiculite to the quartz sand is (80-90): (10-20); the rooting matrix C comprises 65-75% of decomposed coconut coir, 7-15% of perlite, 5-10% of organic fertilizer, 7-15% of vermiculite and 0.1-0.4% of slow release fertilizer by volume percentage.

2. The light simplified seedling raising substrate for date tillering seedlings according to claim 1, wherein the reagent A is 1/4MS salt solution containing 1000-fold diluted carbendazim and 800-fold diluted azoxystrobin;

the reagent B is a mixed solution containing 400mg/L of naphthalene acetic acid solution, 150mg/L of indolebutyric acid solution and 80mg/L of 2, 4-dichlorophenoxyacetic acid solution;

reagent C is a 0.5mg/L solution of naphthylacetic acid.

3. The light simplified seedling raising matrix for the tillered seedlings of date palm according to claim 1, wherein the rooting matrix C comprises 70% of decomposed coconut husk, 10% of perlite, 8% of organic fertilizer, 11.8% of vermiculite and 0.2% of slow release fertilizer in percentage by volume.

4. A method for preparing the light simplified seedling substrate for the tillering seedlings of the date palm according to any one of claims 1 to 3, which is characterized by comprising the following steps:

(1) Preparation of reagent A: preparing 1/4MS salt solution by using sterile water, weighing carbendazim and azoxystrobin with corresponding dilution factors, and dissolving the carbendazim and azoxystrobin in the 1/4MS salt solution to obtain a reagent A;

(2) Preparation of reagent B: weighing naphthalene acetic acid, indolebutyric acid and 2, 4-dichlorophenoxyacetic acid, respectively dissolving the naphthalene acetic acid, the indolebutyric acid and the 2, 4-dichlorophenoxyacetic acid in sodium hydroxide solution, adding sterile water to a certain volume to corresponding concentration, and mixing according to the volume ratio of the naphthalene acetic acid solution, the indolebutyric acid solution and the 2, 4-dichlorophenoxyacetic acid solution of 2:1:1.5 to obtain a reagent B;

(3) Preparation of reagent C: weighing naphthalene acetic acid, completely dissolving by using sodium hydroxide solution, and adding sterile water to fix the volume to the corresponding concentration to obtain a reagent C.

5. The method for preparing a light simplified seedling substrate for date tillering seedlings according to claim 4, wherein in the steps (2) and (3), the molar concentration of the sodium hydroxide solution is 1mol/L.

6. The method for preparing the light simplified seedling substrate for the tillering seedlings of the date palm according to claim 4 or 5, further comprising:

(4) Preparing a fixed substrate B: sterilizing the fixed substrate B, and soaking the fixed substrate B in sterile water for later use;

(5) Preparing a rooting matrix C: uniformly mixing sterilized decomposed coconut husk, perlite, organic fertilizer, vermiculite and slow release fertilizer according to a proportion for standby.

7. A method for using the light simplified seedling substrate for tillering seedlings of date palm according to any one of claims 1 to 3, which is characterized by comprising the following steps:

(1) Pretreating the date tillering seedlings;

(2) Taking down the tillering seedling of the date palm from the parent body, immersing the basal part of the tillering seedling in the reagent A for 15-20min, taking out, and washing with sterile water to obtain the tillering Miao of the date palm;

(3) Soaking the basal part of the tillering seedling I of the date palm in the reagent B for 40-50min, taking out, then planting in the fixed matrix B, planting one plant in each pot, spraying sterile water each day to keep the basal part of the tillering seedling moist, culturing for 30-40 days, and taking out to obtain the tillering Miao of the date palm;

(4) Adding a reagent C into a rooting matrix C, wherein the volume ratio is the rooting matrix C: the reagent C=2:1, then planting the date tillering seedling II, planting one plant in each pot, irrigating 1L of the reagent C every 25-35 days, continuously irrigating 3-5 times, and spraying sterile water during the period to keep the substrate C moist, so as to obtain date tillering seedling with solid root;

(5) And (5) strengthening seedlings and transplanting.

8. The method for using the light simplified seedling substrate for tillering seedlings of date palm according to claim 7, wherein the specific operation of the pretreatment of the tillering seedlings of date palm in the step (1) is as follows: removing the dead branches and basal impurities of the date palm tillers before the date palm tillers are taken down, spraying the whole date palm tillers by mixing 3% thiacloprid microcapsule suspending agent 1500-2000 times diluent and 20% propiconazole emulsifiable concentrate 1200-1500 times diluent, spraying again after 14 days at intervals, and taking down the date palm tillers from the parent body after 7 days.