CN113337382A - High-flux nucleic acid detection system - Google Patents
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- CN113337382A CN113337382A CN202110489787.2A CN202110489787A CN113337382A CN 113337382 A CN113337382 A CN 113337382A CN 202110489787 A CN202110489787 A CN 202110489787A CN 113337382 A CN113337382 A CN 113337382A
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
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Abstract
The invention belongs to the technical field of medical detection, and particularly relates to a high-throughput nucleic acid detection system. The nucleic acid detection system comprises a lofting frame, an optical assembly, a detection assembly and a shell; the lofting frame comprises a sample module support frame (1) and a plurality of sample modules, and the sample modules are arranged on the lofting frame in an array; the sample module is provided with a sample tube placing hole (2) and/or a bag type sample tube placing hole (14) is formed between adjacent sample modules in a plurality of sample modules; the sample module is provided with a heating element (3) for heating the sample tube placing hole (2) or the bag type sample tube placing hole (14); the lofting frame is fixed on the shell, and the optical assembly and the detection assembly are arranged below the lofting frame. The detection system can realize portable high-flux field rapid full-automatic detection, realize one-step sample adding, full-automatic rapid detection of a large amount of samples in small-volume instruments, and has small volume, large flux and simple pretreatment.
Description
Technical Field
The invention belongs to the technical field of medical detection, and particularly relates to a high-throughput nucleic acid detection system.
Background
In the present stage, the screening of a large amount of nucleic acids such as bacteria and viruses is mainly completed by pure manual or manual + semi-mechanical operation in a laboratory, so that one-step sample injection detection cannot be realized, and the screening cannot be performed in a PCR laboratory or on-site screening cannot be performed. Although the existing mobile PCR laboratory like a mobile shelter can be placed on site, the occupied area is large, the laboratory is not flexible, the manufacturing cost is high, the laboratory can be completed by pure manual or manual plus semi-mechanical operation, and one-step sample injection detection cannot be realized. The existing full-automatic nucleic acid detecting instrument has a complicated internal mechanical structure, so that the instrument has small volume but low flux, can not screen a large number of samples on site, increases the flux and has large volume correspondingly.
The detection of bacteria, viruses and the like through samples such as blood, nasopharyngeal swab, human tissues and the like is mainly realized through a nucleic acid extraction detection mode. In the current main detection method, the detection flux is related to the size of the instrument, and generally speaking, the flux is large, the instrument is large, the flux is small, and the instrument is small. For a chip-type detection instrument, single-sample multi-site detection can be realized, if a large number of samples are detected, the volume of the instrument is increased, and a large-flux chip-type detection instrument is not available at present.
The existing full-automatic nucleic acid detection instrument is invented, but the internal mechanical structure is complicated, so that the instrument is small in size but low in flux, a large number of samples cannot be screened on site, the flux is increased, and the instrument size is correspondingly large.
Chinese utility model patent CN212432985U a scanning fluorescence detection device, which comprises in order along the direction of light beam propagation: the excitation light source module is used for generating light beams with at least one wavelength and sending the excitation light beams with one wavelength to the first light path shaping module; the first light path shaping module is used for receiving the excitation light beam and forming a first light spot; the first light spot is used for irradiating the wafer to enable organic matters on the surface of the wafer to generate fluorescence; the light collection module is used for collecting fluorescence generated by organic matters from the surface of the wafer; and the image acquisition module is used for receiving the fluorescence output by the light collection module and imaging. However, the patent can only detect samples with small flux, cannot be used for detecting large flux and a large number of samples, and cannot meet the requirement of simultaneous detection of multiple samples.
Disclosure of Invention
The invention aims to provide a high-flux nucleic acid detection system which can realize portable high-flux field rapid full-automatic detection, realize one-step sample adding, realize full-automatic rapid detection of a large amount of samples in a small-volume instrument, and has small volume, large flux (not less than 100 cases) and simple pretreatment.
In order to achieve the purpose, the invention adopts the following technical scheme:
a high throughput nucleic acid detection system comprising a loft frame, an optical assembly, a detection assembly, and a housing;
the lofting frame comprises a sample module supporting frame and a plurality of sample modules, and the sample modules are arranged on the lofting frame in an array; the sample module is provided with a sample tube placing hole and/or a bag type sample tube placing hole is formed between adjacent sample modules in the plurality of sample modules; the sample module is provided with a heating element for heating the sample tube placing hole and/or the bag type sample tube placing hole;
the optical assembly comprises a light source, a light source light transmitter and a light source optical filter; the light source, the light source light transmitter and the light source optical filter are sequentially arranged on the optical component support frame;
the detection assembly comprises a detector, a detector light transmitter and a detector optical filter, and the detector, the detector light transmitter and the detector optical filter are sequentially arranged on the detection assembly supporting frame;
the lofting frame is fixed on the shell, and the optical assembly and the detection assembly are arranged below the lofting frame.
Further, the loft frame also includes an actuating device disposed above the sample module and a clamp disposed above and adjacent to the actuating device.
Preferably, several sample modules are arranged in an array on the loft frame.
Preferably, the sample module is square or rectangular parallelepiped or cylindrical.
Further, set up the indicator lamp plate on the sample module.
Further, the optical assembly is arranged on the optical assembly guide rail and can move along the optical assembly guide rail.
Further, the detection assembly is arranged on the detection assembly guide rail and can move along the detection assembly guide rail.
Preferably, the heating element is an electric heating wire, a heating film, a ceramic heating sheet, or a semiconductor heating sheet.
Further, the nucleic acid detection system also comprises a lofting frame cover, wherein the lofting frame cover is fixed on the shell, can be opened during lofting and is arranged above the lofting frame.
In the invention, the interior of the sample tube or the bag-type sample tube is divided into two independent cavities, the two cavities are isolated, and the isolation is removed by heating, magnetic force or extrusion to communicate the two cavities.
Further preferably, the number of the optical assemblies is multiple, and the optical assemblies surround the detection assembly in a ring shape or are distributed in a certain rule in the space around the detection assembly.
The light source light transmitter and the detector light transmitter of the present invention use lenses or optical fibers to transmit light of a light source to the reaction tube through the light transmitter, and simultaneously transmit fluorescence of reagents in the reaction tube to a detector (e.g., a camera) or other light receiving devices by using the light transmitter. The lower part of each reaction tube is respectively corresponding to one or a pair of light transmitters, or one light transmitter, and the end parts of the light transmitters are sequentially moved to the bottom of each reaction tube by a driving mechanism to collect fluorescence; it may be a row of light conveyors which are moved in the column direction by a driving mechanism until all the reaction tubes are covered.
The method is mainly used for large-flux screening of bacteria, viruses and the like, and can realize integrated large-flux rapid screening of the samples of the viruses, the bacteria and the like through simple operation.
Compared with the prior art, the invention has the beneficial effects that:
1. high-flux detection with short time and small volume is realized; the portable operation is realized, the instrument is convenient to carry, and the occupied area is small, so that the portable multifunctional coronary disease diagnosis instrument can be used for dealing with sudden public health events at different times and places such as new coronary epidemic situations; one-step application of sample, convenient operation, the training is convenient, can realize that a large amount of samples in time detect, saves personnel, time.
2. The large-flux detection is carried out at one time, the instrument is small in size and convenient to deploy and move, the detection is finished at one time without the help of a moving device or a simple moving device, and an interpretation result is output;
3. the modeling is simple, and the manufacturing cost is low; the required instruments and accessories are simple, the cost of the instruments is reduced, and the whole volume of the instruments is reduced.
Drawings
FIG. 1 is a diagram of an arrangement of sample modules in a lofting frame according to the present invention;
FIG. 2 is a schematic structural diagram of an optical assembly;
FIG. 3 is a schematic structural diagram of a detection assembly;
FIG. 4 is a cross-sectional view of a nucleic acid detecting system according to the present invention;
FIG. 5 is a schematic view of the structure of the nucleic acid detecting system of the present invention;
FIG. 6 is another sectional view of a sectional view of the nucleic acid detecting system of the present invention;
FIG. 7 is a layout view of an optical assembly and a detection assembly of the present invention;
reference numerals:
1. a sample module support frame; 2. a sample tube placement hole; 3. a heating element; 4. a light source; 5. a light source light transmitter; 6. a light source filter; 7. an optical component support frame; 8. a detector; 9. a detector light transmitter; 10. a detector filter; 11. a detection assembly support frame; 12. an actuating device; 13. a clamp; 14. a bag type sample tube placing hole; 15. an indicator light panel; 16. a lofting frame cover; 17. a housing.
Detailed Description
The invention is described in further detail below with reference to the figures and the detailed description.
Example 1
As shown in fig. 4 and 5, a high throughput nucleic acid detection system includes a loft frame, an optical assembly, a detection assembly, and a housing;
as shown in fig. 1, the lofting frame comprises a sample module support frame 1 and a plurality of sample modules, wherein the sample modules are arranged on the sample module support frame 1 in an array, sample tube placing holes 2 are formed in the sample modules, and heating elements 3 are arranged around the sample tube placing holes 2;
as shown in fig. 2, the optical assembly includes a light source 4, a light source light transmitter 5, and a light source filter 6; the light source 4, the light source light transmitter 5 and the light source optical filter 6 are sequentially arranged on the optical component support frame 7;
as shown in fig. 3, the detection assembly includes a detector 8, a detector light transmitter 9 and a detector filter 10, the detector 8, the detector light transmitter 9 and the detector filter 10 are sequentially disposed on a detection assembly support frame 11;
the lofting frame is fixed on the shell, and the optical assembly and the detection assembly are arranged below the lofting frame.
The sample module is cube-shaped.
And an indicator lamp panel 15 is arranged on the sample module. The indicator lamp plate is used for indicating whether a sample detection result in the sample module is positive or not, and when the sample detection result is positive, the indicator lamp is turned on.
The heating element 3 is an electric heating wire.
The invention is realized by skillfully arranging the closed nucleic acid reaction tube, the detection component, the optical component and the large-batch reaction tube lofting frame in the shell. The enclosed nucleic acid reaction tube can realize the liquid flow in the completely enclosed reaction tube by the heating operation in the detector, and complete the biochemical reaction in the completely enclosed tube. The rapid extraction reagent and the rapid PCR reagent are arranged in the reaction tube.
The reaction tubes in large batches (not less than 100 cases) are arranged in an array mode, so that the size of a final instrument can be within 300mm x 480 mm; the detection assembly is positioned below the reaction tube lofting frame and can perform detection in a mode of taking pictures wholly or locally by a camera; the optical assembly employs an arc lamp, an LED lamp, a tungsten lamp, or the like as a light source.
The closed nucleic acid reaction tube is internally divided into two independent cavities for storing different reagents, the two cavities are separated by physical measures, and the two cavities can be communicated by a thermal method, a magnetomechanical method or other mechanical methods to complete reagent mixing.
The optical component comprises a light source, a light transmitter system and a wavelength selection mechanism, exciting light is emitted, a detection substance in the reagent tube is excited to emit fluorescence, the detection component captures the fluorescence, and result interpretation processing is carried out.
The invention controls the temperature of the heating element and realizes the nucleic acid amplification reaction process in the reagent tube in a temperature cycle mode.
Compared with the traditional detection, the invention can realize the one-time detection of the multi-flux large target surface, has no moving mechanism among the excitation, emission and detection components, greatly reduces the complexity of the system due to the stable structural relationship, improves the detection efficiency of the system, reduces the weight of the instrument due to the absence of the moving mechanism, reduces the volume of the instrument and is suitable for rapid deployment.
After the reaction tube is filled with the sample such as throat swab, the reaction tube is completely sealed by screwing down the reaction tube. The reaction tube is placed in the invention, and the liquid flows in the closed tube through heating or other simple operations, so that the operations of nucleic acid rapid extraction, rapid PCR reaction and the like are completed.
Example 2
As shown in fig. 5 and 6, a high throughput nucleic acid detection system includes a loft frame, an optical assembly, a detection assembly, and a housing;
as shown in fig. 1, the lofting frame comprises a sample module support frame 1 and a plurality of sample modules, wherein the sample modules are arranged on the sample module support frame 1 in an array manner, and heating elements 3 are arranged in the sample modules; the lofting frame further comprises an actuating device 12 and a clamp 13, the actuating device 12 is arranged above the sample modules, the clamp 13 is arranged above the sample modules and adjacent to the actuating device 12, bag-type sample tube placing holes 14 are formed between adjacent sample modules in the plurality of sample modules, and the sample bags in the bag-type sample tube placing holes can be heated by the heating elements.
As shown in fig. 2, the optical assembly includes a light source 4, a light source light transmitter 5, and a light source filter 6; the light source 4, the light source light transmitter 5 and the light source optical filter 6 are sequentially arranged on the optical component support frame 7;
as shown in fig. 3, the detection assembly includes a detector 8, a detector light transmitter 9 and a detector filter 10, the detector 8, the detector light transmitter 9 and the detector filter 10 are sequentially disposed on a detection assembly support frame 11;
the lofting frame is fixed on the shell, and the optical assembly and the detection assembly are arranged below the lofting frame.
The sample module is cube-shaped. And an indicator lamp panel 15 is arranged on the sample module. The indicator lamp plate is used for indicating whether the sample in the sample module is positive or not, and when the sample module is positive, the indicator lamp is turned on.
The heating element 3 is a ceramic heating plate.
The reaction hose is composed of two sections, a small through hole with the diameter not larger than 0.5mm is arranged between the sections, one end of the hole is sealed (hot melting materials can also be used for sealing, when the reaction hose is heated, the hot melting materials are melted, so that the through hole is communicated with the upper section and the lower section), substances such as a filter element, a filter membrane and the like are placed above the tube hole at the upper section, reagents such as nucleic acid extracting solution and the like can be stored in the section below the cover, reagents such as PCR reaction solution and the like can be stored in the second section, the thickness of the bottom of the reaction hose is not smaller than 1mm, and the bottom of the reaction hose is transparent, so that the detection is convenient.
The soft bag type reaction tube is placed in the bag type sample tube placing hole, at the moment, the upper section and the lower section of the reaction tube form an included angle of 90 degrees, the upper section is horizontally placed on the upper surface of the sample module, an actuating device is arranged above the upper section, the lower section is placed in the bag type sample tube placing hole in a vertical state and is positioned between the heating elements of the two modules, and a clamp is arranged above the joint between the upper section and the lower section.
The actuating device is used for pressing the liquid of the upper section to flow into the lower section; the clamp is used for obstructing the first section and the second section from liquid. The heating element can also be controlled to heat the fused sealing part of the reaction tube so as to communicate the upper section with the lower section. The clamp is used for blocking two sections of liquid.
The specific operation steps are as follows: after the upper section is added with samples such as throat swabs and the like, the tube cover is closed and placed in the instrument; the actuating device is used for enabling the upper section liquid to flow into the lower section, the clamp is used for blocking the two sections of liquid, the lower section liquid is heated, the reagent starts to react, and finally the signal is detected below through the detection device. Or the upper section of liquid flows into the lower section of liquid through heating and cooling, the two sections of liquid are blocked by the clamp, the lower section of liquid is heated, the reagent starts to react, and finally, the signal is detected below through the detection device.
The reaction tubes in large batches (not less than 100 cases) are arranged in an array mode, and the size of a final instrument can be within 300 mm-480 mm.
Example 3
A high throughput nucleic acid detection system comprising a loft frame, an optical assembly, a detection assembly, and a housing;
the lofting frame comprises a sample module support frame 1 and a plurality of sample modules, wherein the sample modules are arranged on the sample module support frame 1 in an array manner, sample tube placing holes 2 are formed in the sample modules, and heating elements 3 are arranged around the sample tube placing holes 2; the lofting frame further comprises an actuating device 12 and a clamp 13, the actuating device 12 is arranged above the sample modules, the clamp 13 is arranged above the sample modules and adjacent to the actuating device 12, bag-type sample tube placing holes 14 are formed between adjacent sample modules in the plurality of sample modules, and the sample bags in the bag-type sample tube placing holes can be heated by the heating elements.
The optical assembly comprises a light source 4, a light source light transmitter 5 and a light source filter 6; the light source 4, the light source light transmitter 5 and the light source optical filter 6 are sequentially arranged on the optical component support frame 7;
the detection assembly comprises a detector 8, a detector light transmitter 9 and a detector optical filter 10, wherein the detector 8, the detector light transmitter 9 and the detector optical filter 10 are sequentially arranged on a detection assembly supporting frame 11;
the lofting frame is fixed on the shell, and the optical assembly and the detection assembly are arranged below the lofting frame.
The sample module is cube-shaped.
And an indicator lamp panel 15 is arranged on the sample module. The indicator lamp plate is used for indicating whether the sample in the sample module is positive or not, and when the sample module is positive, the indicator lamp is turned on.
The heating element 3 is a semiconductor heating plate.
This embodiment is a multifunctional nucleic acid detecting system, and a general-purpose reaction tube or a soft bag reaction tube may be used.
The procedure was the same as in example 1 when a general closed reaction tube was used, and the procedure was the same as in example 2 when a soft bag-type reaction tube was used.
Example 4
Compared with the embodiment 1, the high-throughput nucleic acid detecting system of the embodiment further comprises a lofting frame cover, wherein the lofting frame cover is fixed on the shell and arranged above the lofting frame and used for covering the lofting frame cover on the lofting frame after the reaction tube is placed in the lofting frame.
Similarly, in embodiments 2-3, a loft cover may also be included.
Example 5
Compared with example 1, the high-throughput nucleic acid detecting system of this embodiment further includes an optical assembly guide rail and a detecting assembly guide rail. The two ends of the optical assembly guide rail and the detection assembly guide rail are fixed on the shell. The optical assembly is arranged on the optical assembly guide rail and can move along the optical assembly guide rail; the detection assembly is arranged on the detection assembly guide rail and can move along the detection assembly guide rail.
Similarly, the embodiments 1-4 can further include an optical assembly guide rail and a detection assembly guide rail.
Example 6
Compared with embodiment 1, the number of the optical assemblies in this embodiment is multiple, the detection assembly is surrounded in a ring shape, and the optical assembly and the detection assembly can share one optical filter, as shown in fig. 7; the optical filters can also be used separately, i.e. the optical assembly still comprises a light source, a light source light transmitter and a light source filter, the detection assembly still comprises a detector, a detector light transmitter and a detector filter, and the optical assembly is annular to surround the detection assembly in the center.
Similarly, the arrangement of the optical assembly and the detection assembly described above can be further adopted in embodiments 1 to 5.
Conventional technical knowledge in the art can be used for the details which are not described in the present invention.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and are not limited. Although the present invention has been described in detail with reference to the embodiments, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted without departing from the spirit and scope of the invention as defined in the appended claims.
Claims (10)
1. A high throughput nucleic acid detection system, comprising a loft rack, an optical assembly, a detection assembly, and a housing;
the lofting frame comprises a sample module support frame (1) and a plurality of sample modules, and the sample modules are arranged on the lofting frame in an array; the sample module is provided with a sample tube placing hole (2) and/or a bag type sample tube placing hole (14) is formed between adjacent sample modules in a plurality of sample modules; the sample module is provided with a heating element (3) for heating the sample tube placing hole (2) and/or the bag type sample tube placing hole (14);
the lofting frame is fixed on the shell, and the optical assembly and the detection assembly are arranged below the lofting frame.
2. The high throughput nucleic acid detection system of claim 1, wherein the optical assembly comprises a light source (4), a light source light transmitter (5), and a light source filter (6); the light source (4), the light source light transmitter (5) and the light source optical filter (6) are sequentially arranged on the optical component support frame (7);
the detection assembly comprises a detector (8), a detector light transmitter (9) and a detector light filter (10), wherein the detector (8), the detector light transmitter (9) and the detector light filter (10) are sequentially arranged on a detection assembly supporting frame (11).
3. The high throughput nucleic acid detection system of claim 1, wherein the loft frame further comprises an actuation device (12) and a gripper (13), the actuation device (12) being disposed above the sample module, the gripper (13) being disposed above the sample module and adjacent to the actuation device (12).
4. The high-throughput nucleic acid detection system of claim 1, wherein the sample module is square, rectangular or cylindrical, and an indicator lamp panel (15) is disposed on the sample module.
5. The high throughput nucleic acid detection system of claim 1, wherein the interior of the sample tube or bag sample tube is divided into two separate chambers, the two chambers being separated by a spacer, the spacer being removed by heating, magnetic force or squeezing, to connect the two chambers.
6. The high throughput nucleic acid detection system of claim 1, wherein the optical assembly is disposed on and movable along an optical assembly guide rail, and the detection assembly is disposed on and movable along a detection assembly guide rail.
7. The high throughput nucleic acid detection system of claim 1, wherein the light source light transmitter and the detector light transmitter employ lenses or optical fibers.
8. The high throughput nucleic acid detecting system according to claim 1, wherein the heating element (3) is an electric heating wire, a heating membrane, a ceramic heating sheet or a semiconductor heating sheet.
9. The high throughput nucleic acid detection system of claim 1, further comprising a loft cover (16), the loft cover (16) being secured to the housing and disposed above the loft.
10. The high throughput nucleic acid detection system of claim 1, wherein the number of optical assemblies is one or more, and the detection assemblies are arranged in a ring shape to surround the center.
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| CN202110489787.2A CN113337382A (en) | 2021-05-06 | 2021-05-06 | High-flux nucleic acid detection system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113866139A (en) * | 2021-09-06 | 2021-12-31 | 北京谊安和景生物科技有限公司 | High-throughput nucleic acid detection optical system |
| CN116496880A (en) * | 2023-04-26 | 2023-07-28 | 南京华银医学检验所有限公司 | Fluorescent quantitative PCR instrument |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109929751A (en) * | 2019-04-23 | 2019-06-25 | 苏州合惠生物科技有限公司 | A kind of novel fluorescence quantitative augmentation detection instrument |
| US20200355715A1 (en) * | 2019-05-06 | 2020-11-12 | University Of Prince Edward Island | Portable field testing apparatus and method |
| CN111979094A (en) * | 2020-08-28 | 2020-11-24 | 中国科学院苏州生物医学工程技术研究所 | Nucleic acid detection device |
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2021
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| CN109929751A (en) * | 2019-04-23 | 2019-06-25 | 苏州合惠生物科技有限公司 | A kind of novel fluorescence quantitative augmentation detection instrument |
| US20200355715A1 (en) * | 2019-05-06 | 2020-11-12 | University Of Prince Edward Island | Portable field testing apparatus and method |
| CN111979094A (en) * | 2020-08-28 | 2020-11-24 | 中国科学院苏州生物医学工程技术研究所 | Nucleic acid detection device |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113866139A (en) * | 2021-09-06 | 2021-12-31 | 北京谊安和景生物科技有限公司 | High-throughput nucleic acid detection optical system |
| CN116496880A (en) * | 2023-04-26 | 2023-07-28 | 南京华银医学检验所有限公司 | Fluorescent quantitative PCR instrument |
| CN116496880B (en) * | 2023-04-26 | 2023-10-03 | 南京华银医学检验所有限公司 | Fluorescent quantitative PCR instrument |
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