CN113185973A - Preparation method of zero-dimensional nano carbon material/origami structure deoxyribonucleic acid composite material - Google Patents
Preparation method of zero-dimensional nano carbon material/origami structure deoxyribonucleic acid composite material Download PDFInfo
- Publication number
- CN113185973A CN113185973A CN202110369392.9A CN202110369392A CN113185973A CN 113185973 A CN113185973 A CN 113185973A CN 202110369392 A CN202110369392 A CN 202110369392A CN 113185973 A CN113185973 A CN 113185973A
- Authority
- CN
- China
- Prior art keywords
- graphene quantum
- dispersion liquid
- dna
- quantum dot
- origami
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
- C09K11/08—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials
- C09K11/65—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials containing carbon
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y20/00—Nanooptics, e.g. quantum optics or photonic crystals
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
-
- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01B—NON-METALLIC ELEMENTS; COMPOUNDS THEREOF; METALLOIDS OR COMPOUNDS THEREOF NOT COVERED BY SUBCLASS C01C
- C01B32/00—Carbon; Compounds thereof
- C01B32/15—Nano-sized carbon materials
- C01B32/182—Graphene
- C01B32/194—After-treatment
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Nanotechnology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Materials Engineering (AREA)
- Physics & Mathematics (AREA)
- Inorganic Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Condensed Matter Physics & Semiconductors (AREA)
- Composite Materials (AREA)
- Manufacturing & Machinery (AREA)
- Biophysics (AREA)
- Optics & Photonics (AREA)
- Carbon And Carbon Compounds (AREA)
Abstract
A preparation method of a zero-dimensional nanocarbon material/folded paper structure deoxyribonucleic acid composite material comprises the steps of respectively preparing folded paper DNA dispersion liquid and graphene quantum dot water dispersion liquid, fully mixing the folded paper DNA dispersion liquid and the graphene quantum dot water dispersion liquid for reaction, and then concentrating and dialyzing the reacted mixed solution to remove redundant graphene quantum dots to obtain the composite material. According to the invention, the Graphene Quantum Dots (GQDs) and the paper folding DNA are coated on the surface of the paper folding DNA by utilizing the electrostatic adsorption effect of the graphene quantum dots and the paper folding DNA, so that the graphene quantum dots are attached to the surface of the paper folding DNA structure, and the environmental adaptability of the paper folding DNA is improved.
Description
Technical Field
The invention relates to a technology in the field of deoxyribonucleic acid engineering, in particular to a preparation method of a zero-dimensional nano carbon material/origami structure deoxyribonucleic acid composite material.
Background
The paper folding DNA is a two-dimensional or three-dimensional DNA nano structure which can be formed by self-assembly by utilizing the self-assembly and sequence programmability of DNA molecules and designing the hybridization relationship among DNA chains. The metal nano-structure and the pattern can be further constructed by taking the metal nano-structure as a template material. However, the existing technical means for preparing the folded paper DNA composite material mostly adopts noble metal ions (gold ions, silver ions and the like) or semiconductor materials (silicon, silicon dioxide and the like) to be compounded with the folded paper DNA, and the composite material prepared by the technology has poor biocompatibility and low environmental friendliness.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a preparation method of a zero-dimensional nanocarbon material/origami structure deoxyribonucleic acid composite material, which is characterized in that Graphene Quantum Dots (GQDs) and origami DNA (deoxyribonucleic acid) electrostatic adsorption are utilized to coat the graphene quantum dots on the surface of the origami DNA, so that the graphene quantum dots are attached to the surface of the origami DNA structure, and the environmental adaptability of the origami DNA is improved.
The invention is realized by the following technical scheme:
the invention relates to a preparation method of a zero-dimensional nanocarbon material/folded paper structure deoxyribonucleic acid composite material.
The volume ratio of the folded paper DNA dispersion liquid to the graphene quantum dot water dispersion liquid is 0.5: 1-2: 1.
The paper folding DNA dispersion liquid is prepared by placing paper folding DNA obtained by regulation and control in a Mg-containing atmosphere2+The buffer solution is dispersed, and the concentration of the buffer solution is 0.1 nM-1 nM.
The graphene quantum dot water dispersion liquid is obtained by dispersing graphene quantum dots with the transverse size smaller than 40nm in deionized water, and the concentration of the graphene quantum dot water dispersion liquid is 0.01-1 mg/mL.
The intensive mixing reaction is as follows: stirring for 10 s-10 min under the condition that the stirring speed is 120-360 r/min.
Technical effects
The invention integrally solves the problems that the prior art can only modify the DNA of the origami by metal ions or semiconductor materials, and the introduction of heavy metals increases the biocompatibility of the composite material and has low environmental friendliness. Compared with the prior art, the invention adopts the zero-dimensional nano material with excellent physicochemical characteristics, such as low toxicity, good water dispersibility, low chemical activity, stable fluorescence property and the like, namely the graphene quantum dot is compounded with the folded paper DNA, so that the folded paper DNA is endowed with the corresponding physicochemical characteristics of the graphene quantum dot.
Drawings
FIG. 1 is an AFM height image of the graphene quantum dot/square origami DNA composite prepared in example 1;
fig. 2 is an AFM phase diagram of the graphene quantum dot/square paper folding DNA composite material prepared in example 1.
Detailed Description
Example 1
The embodiment comprises the following steps: preparation of a composition containing Mg2+0.1nM of square origami DNA dispersion in buffer; preparing 0.05mg/mL graphene quantum dot water dispersion liquid with the transverse dimension smaller than 40 nm; and (3) mixing the square folded paper DNA dispersion liquid and the graphene quantum dot water dispersion liquid according to the volume ratio of 1: 1 mixing, stirring for 10s under the condition that the stirring speed is 360 r/min for full reaction, and passing the mixed solution after the reaction through a 30K ultrafiltration tubeAnd (5) concentrating, dialyzing, and removing redundant graphene quantum dots to obtain a final product.
As shown in fig. 1 and 2, the graphene quantum dot/origami DNA composite material has dual morphological characteristics of the granular graphene quantum dot and the square origami DNA.
Example 2
The embodiment comprises the following steps: preparation of a composition containing Mg2+1nM of square origami DNA dispersion in buffer; preparing 1mg/mL graphene quantum dot water dispersion liquid with the transverse dimension smaller than 40 nm; and (3) mixing the square folded paper DNA dispersion liquid and the graphene quantum dot water dispersion liquid according to the volume ratio of 2:1, stirring for 5min under the condition that the stirring speed is 200 r/min for full reaction, concentrating the reacted mixed solution through a 30K ultrafiltration tube, dialyzing, and removing redundant graphene quantum dots to obtain a final product.
Example 3
The embodiment comprises the following steps: preparation of a composition containing Mg2+0.5nM triangular origami DNA dispersion in buffer; preparing 0.01mg/mL graphene quantum dot water dispersion liquid with the transverse dimension smaller than 40 nm; and (3) mixing the square origami DNA dispersion solution and the graphene quantum dot water dispersion solution according to the volume ratio of 0.5:1, stirring for 10min under the condition that the stirring speed is 120 r/min for full reaction, concentrating the reacted mixed solution through a 30K ultrafiltration tube, dialyzing, and removing redundant graphene quantum dots to obtain a final product.
The invention adopts zero-dimensional nano material with excellent physicochemical characteristics, such as low toxicity, good water dispersibility, low chemical activity, stable fluorescence property and the like, namely graphene quantum dots to be compounded with folded paper DNA, and endows the folded paper DNA with corresponding physical and chemical characteristics of the graphene quantum dots.
The foregoing embodiments may be modified in many different ways by those skilled in the art without departing from the spirit and scope of the invention, which is defined by the appended claims and all changes that come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Claims (5)
1. A preparation method of a zero-dimensional nanocarbon material/folded paper structure deoxyribonucleic acid composite material is characterized in that folded paper DNA dispersion liquid and graphene quantum dot water dispersion liquid are respectively prepared and fully mixed for reaction, and then the reacted mixed solution is concentrated and dialyzed to remove redundant graphene quantum dots to obtain the composite material;
the volume ratio of the folded paper DNA dispersion liquid to the graphene quantum dot water dispersion liquid is 0.5: 1-2: 1.
2. The method for preparing zero-dimensional nanocarbon material/origami-structured deoxyribonucleic acid composite material according to claim 1, wherein the origami DNA dispersion is prepared by placing origami DNA obtained by regulation and control in a Mg-containing atmosphere2+The buffer solution is dispersed, and the concentration of the buffer solution is 0.1 nM-1 nM.
3. The method for preparing the zero-dimensional nanocarbon material/origami structure deoxyribonucleic acid composite material according to claim 1, wherein the graphene quantum dot water dispersion liquid is obtained by dispersing graphene quantum dots with the transverse dimension of less than 40nm in deionized water, and the concentration of the graphene quantum dots is 0.01 mg/mL-1 mg/mL.
4. The method for preparing the zero-dimensional nano carbon material/origami structure deoxyribonucleic acid composite material according to claim 1, wherein the intensive mixing reaction is as follows: stirring for 10 s-10 min under the condition that the stirring speed is 120-360 r/min.
5. The method for preparing the zero-dimensional nano carbon material/origami structure deoxyribonucleic acid composite material according to any one of claims 1 to 4, which is characterized by being realized in any one of the following modes:
firstly, preparing Mg-containing2+0.1nM of square origami DNA dispersion in buffer; preparing 0.05mg/mL graphene quantum dot water dispersion liquid with the transverse dimension smaller than 40 nm; and (3) mixing the square folded paper DNA dispersion liquid and the graphene quantum dot water dispersion liquid according to the volume ratio of 1: 1 mixing, stirring for 10s under the condition that the stirring speed is 360 r/min for full reaction, and subjecting the reacted mixed solution to reactionConcentrating by using a 30K ultrafiltration tube, dialyzing, and removing redundant graphene quantum dots to obtain a final product;
② preparing Mg-containing2+1nM of square origami DNA dispersion in buffer; preparing 1mg/mL graphene quantum dot water dispersion liquid with the transverse dimension smaller than 40 nm; and (3) mixing the square folded paper DNA dispersion liquid and the graphene quantum dot water dispersion liquid according to the volume ratio of 2:1, mixing, stirring for 5min under the condition that the stirring speed is 200 r/min for full reaction, concentrating the reacted mixed solution through a 30K ultrafiltration tube, dialyzing, and removing redundant graphene quantum dots to obtain a final product;
③ preparing Mg-containing2+0.5nM triangular origami DNA dispersion in buffer; preparing 0.01mg/mL graphene quantum dot water dispersion liquid with the transverse dimension smaller than 40 nm; and (3) mixing the square origami DNA dispersion solution and the graphene quantum dot water dispersion solution according to the volume ratio of 0.5:1, stirring for 10min under the condition that the stirring speed is 120 r/min for full reaction, concentrating the reacted mixed solution through a 30K ultrafiltration tube, dialyzing, and removing redundant graphene quantum dots to obtain a final product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110369392.9A CN113185973B (en) | 2021-04-06 | 2021-04-06 | Preparation method of zero-dimensional nano carbon material/paper folding structure deoxyribonucleic acid composite material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110369392.9A CN113185973B (en) | 2021-04-06 | 2021-04-06 | Preparation method of zero-dimensional nano carbon material/paper folding structure deoxyribonucleic acid composite material |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113185973A true CN113185973A (en) | 2021-07-30 |
| CN113185973B CN113185973B (en) | 2023-04-28 |
Family
ID=76975198
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110369392.9A Active CN113185973B (en) | 2021-04-06 | 2021-04-06 | Preparation method of zero-dimensional nano carbon material/paper folding structure deoxyribonucleic acid composite material |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113185973B (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103159170A (en) * | 2013-03-06 | 2013-06-19 | 中国科学院苏州纳米技术与纳米仿生研究所 | Three-dimensional nano-structure construction method |
| CN104133053A (en) * | 2014-05-28 | 2014-11-05 | 上海纳米技术及应用国家工程研究中心有限公司 | Detection method using DNA nano-origami structure as signal amplification probe |
| CN106620725A (en) * | 2017-02-16 | 2017-05-10 | 国家纳米科学中心 | Optical and opto-acoustic integration dual-mode molecular image probe as well as preparation method and application thereof |
| US20170152385A1 (en) * | 2015-11-27 | 2017-06-01 | Institute Of Process Engineering, Chinese Academy Of Sciences | Method for preparation of carbon quantum dots and application |
| CN109706221A (en) * | 2018-09-21 | 2019-05-03 | 湘潭大学 | A method of the resonant light scattering probe combination CHA technology quantitative detection mutant DNA based on GQDs |
| CN110243792A (en) * | 2019-06-11 | 2019-09-17 | 山东师范大学 | A kind of fluorescence chemical sensor and its detection method and application based on quantum dot and tetrahedron DNA structure |
| EP3699142A1 (en) * | 2019-02-20 | 2020-08-26 | Instytut Chemii Fizycznej Polskiej Akademii Nauk | Method of immobilization nucleic acid structure on surface of graphene, graphene modified by immobilization of nucleic acid structure and its use |
| CN111638330A (en) * | 2020-06-10 | 2020-09-08 | 青岛农业大学 | Biosensor for detecting salmonella typhimurium and application thereof |
| CN112129788A (en) * | 2020-09-16 | 2020-12-25 | 南京大学 | Super-resolution microscale based on quantum dot and DNA origami nano-assembly structure |
-
2021
- 2021-04-06 CN CN202110369392.9A patent/CN113185973B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103159170A (en) * | 2013-03-06 | 2013-06-19 | 中国科学院苏州纳米技术与纳米仿生研究所 | Three-dimensional nano-structure construction method |
| CN104133053A (en) * | 2014-05-28 | 2014-11-05 | 上海纳米技术及应用国家工程研究中心有限公司 | Detection method using DNA nano-origami structure as signal amplification probe |
| US20170152385A1 (en) * | 2015-11-27 | 2017-06-01 | Institute Of Process Engineering, Chinese Academy Of Sciences | Method for preparation of carbon quantum dots and application |
| CN106620725A (en) * | 2017-02-16 | 2017-05-10 | 国家纳米科学中心 | Optical and opto-acoustic integration dual-mode molecular image probe as well as preparation method and application thereof |
| CN109706221A (en) * | 2018-09-21 | 2019-05-03 | 湘潭大学 | A method of the resonant light scattering probe combination CHA technology quantitative detection mutant DNA based on GQDs |
| EP3699142A1 (en) * | 2019-02-20 | 2020-08-26 | Instytut Chemii Fizycznej Polskiej Akademii Nauk | Method of immobilization nucleic acid structure on surface of graphene, graphene modified by immobilization of nucleic acid structure and its use |
| CN110243792A (en) * | 2019-06-11 | 2019-09-17 | 山东师范大学 | A kind of fluorescence chemical sensor and its detection method and application based on quantum dot and tetrahedron DNA structure |
| CN111638330A (en) * | 2020-06-10 | 2020-09-08 | 青岛农业大学 | Biosensor for detecting salmonella typhimurium and application thereof |
| CN112129788A (en) * | 2020-09-16 | 2020-12-25 | 南京大学 | Super-resolution microscale based on quantum dot and DNA origami nano-assembly structure |
Non-Patent Citations (1)
| Title |
|---|
| ANSHULA TANDON: "DNA structures embedded with functionalized nanomaterials for biophysical applications", 《JOURNAL OFTHE KOREAN PHYSICAL SOCIETY (2021)》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113185973B (en) | 2023-04-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Wang et al. | Dispersing multi-walled carbon nanotubes with water–soluble block copolymers and their use as supports for metal nanoparticles | |
| Wang et al. | Graphene-based materials: fabrication, characterization and application for the decontamination of wastewater and wastegas and hydrogen storage/generation | |
| Meng et al. | Advanced technology for functionalization of carbon nanotubes | |
| Khajeh et al. | Nanoadsorbents: classification, preparation, and applications (with emphasis on aqueous media) | |
| Fu et al. | Facile preparation of RuO2/CNT catalyst by a homogenous oxidation precipitation method and its catalytic performance | |
| Zhang et al. | The formation of magnetite nanoparticles on the sidewalls of multi-walled carbon nanotubes | |
| KR20110099731A (en) | Method for producing metal nanoparticles and nanoparticles obtained in this way and use thereof | |
| Van der Bruggen | The separation power of nanotubes in membranes: a review | |
| CN1424149A (en) | Method for coating mono-metal particles on carbon nano tube surface | |
| Tsai et al. | Enhancing hydrogen storage on carbon nanotubes via hybrid chemical etching and Pt decoration employing supercritical carbon dioxide fluid | |
| Li et al. | Non-covalently functionalized carbon nanostructures for synthesizing carbon-based hybrid nanomaterials | |
| Kang et al. | Nanocomposite membranes containing positively polarized gold nanoparticles for facilitated olefin transport | |
| Kumar et al. | Phase transfer of platinum nanoparticles from aqueous to organic solutions using fatty amine molecules | |
| Saleh | Properties of nanoadsorbents and adsorption mechanisms | |
| Gong et al. | Advancing selective copper detection in water: Innovative electrode utilizing surface ion-imprinted polymer | |
| Kang et al. | Synthesis and characterization of polyoxometalate nanowires based on a novel microemulsion process | |
| Ravindran et al. | Self-assembly of ZnO nanoparticles to electrostatic coordination sites of functionalized carbon nanotubes | |
| CN113185973A (en) | Preparation method of zero-dimensional nano carbon material/origami structure deoxyribonucleic acid composite material | |
| Vimalanathan et al. | Dynamic thin films in controlling the fabrication of nanocarbon and its composites | |
| CN107893169A (en) | A kind of preparation method of CNT and graphene hybrid reinforced metal-matrix composites | |
| CN107913668B (en) | Nano composite material with adsorption and catalytic degradation functions and preparation method and application thereof | |
| Zhang et al. | Using Cellulose Nanocrystal as Adjuvant to Improve the Dispersion Ability of Multilayer Graphene in Aqueous Suspension | |
| Wang et al. | Dispersion of graphene in aqueous solution | |
| Rabbani et al. | Comparison of some gold/carbon nanotube composites prepared by control of electrostatic interaction | |
| Zhao et al. | The sustainable, one-pot and high-yield synthesis of ultrafine carbonaceous nanospheres with high anionic separation efficiency |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |