CN1128225C - Prepn process of chitinamine oligosaccharide - Google Patents
Prepn process of chitinamine oligosaccharide Download PDFInfo
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- CN1128225C CN1128225C CN 01107822 CN01107822A CN1128225C CN 1128225 C CN1128225 C CN 1128225C CN 01107822 CN01107822 CN 01107822 CN 01107822 A CN01107822 A CN 01107822A CN 1128225 C CN1128225 C CN 1128225C
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- chitinamine
- oligosaccharide
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- preparing
- curing
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The present invention relates to a method for preparing chitinamine oligosaccharide. The method comprises the following steps: chitinamine is dissolved with acetic acid; enzymes are added to the obtained solution for reaction; after the reaction is finished, hydrochloric acid is added; finally, spray drying operation is carried out. The method for preparing chitinamine oligosaccharide is characterized in that the enzymes are hemicellulase, cellulase and beta amylase; in the reaction process, the decompression evaporation is carried out. When the method is used for degrading the chitinamine, chitinamine oligosaccharide whose polymerization degree is from 2 to 12 is obtained, and the average molecular weight is 1500. The chitinamine oligosaccharide is used for inhibiting tumour, preventing and curing hepatosis, improving functions of the intestinal tract, preventing and curing senile diseases, curing skin injury, improving the environment of body fluid, resisting bacteria, etc.
Description
The present invention relates to a kind of method for preparing chitinamine oligosaccharide.
Chitosan claims chitosan again, is the derivative after chitin takes off acetyl, is distributed widely in the crust of shrimp, crab, lobster etc.As the chitinamine oligosaccharide of one of chitosan derivative, shift at anticancer, regulate cell fission, suppress bacterium and fungi growth, excite the aspects such as element with fixed attention of inducing the higher plant endosome to produce plant to have good effect.Chitosan degraded is mainly contained salt acid system, enzyme process, mainly be that the polymerization degree is the oligose (its physiologically active is relatively poor) below 2 because the salt acid degradation obtains, and the enzymic degradation reaction conditions is relatively gentleer, is easy to control, and productive rate is higher, thereby is widely adopted.But now also do not find a kind of suitable enzyme to be enough to make it to be degraded to molecular-weight average about 1500, most of molecular-weight average is more than 10,000, and wherein the polymerization degree is that the yield of oligosaccharide of 3-10 is extremely low.
The purpose of this invention is to provide a kind of method for preparing chitinamine oligosaccharide, it can overcome the above-mentioned shortcoming of prior art.
A kind of method for preparing chitinamine oligosaccharide, comprise with acetic acid chitosan is dissolved, the enzyme-added reaction in the solution of gained, reaction finishes the back and adds NaOH, last spraying drying, it is characterized in that described enzyme is hemicellulase, cellulase and βDian Fenmei, in reaction process, carry out reduction vaporization.
The present invention utilizes the bioactive enzyme chitosan of degrading, obtaining the polymerization degree is the individual chitinamine oligosaccharide of 2-12, molecular-weight average is 1500, in order to suppress tumour, prevent and treat hepatopathy, improve intestinal function, prevent and treat senile disease, treat skin injury, to improve body fluid environment and antibiotic etc.
Below by embodiment the present invention is described.
In 1 liter 50 ℃ warm water, add 100 gram chitosan (purity is 90% (mass percent)), swelling 30 minutes.The CH that adds 1N (equivalent concentration)
3About 1 liter of COOH dissolves it fully.The placement of spending the night, PH is 3.5.Add hemicellulase 20ml, stirring reaction 2 hours; Reduction vaporization CH wherein
3COOH is controlled at about 5.0 PH; Add cellulase 100ml again, stirring reaction 6 hours; Continue reduction vaporization CH
3COOH makes PH rise to 7.0, adds beta-amylase, reacts 1 hour.Add NaOH, transferring PH is about 8.0.Solution is carried out spraying drying, get thick product.85 ℃ of dryings 2 hours, vacuum packaging promptly got chitinamine oligosaccharide with thick product.
The volume of used warm water can be in ± 20% change in the present embodiment.Added CH
3It is 1-3 that the concentration of COOH can make the PH of solution; The consumption of various enzymes can above-mentioned consumption 15% in the change.
Claims (1)
1, a kind of method for preparing chitinamine oligosaccharide, comprise with acetic acid chitosan is dissolved, the enzyme-added reaction in the solution of gained, reaction finishes the back and adds NaOH, last spraying drying, it is characterized in that described enzyme is hemicellulase, cellulase and βDian Fenmei, in reaction process, carry out reduction vaporization.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01107822 CN1128225C (en) | 2001-02-24 | 2001-02-24 | Prepn process of chitinamine oligosaccharide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01107822 CN1128225C (en) | 2001-02-24 | 2001-02-24 | Prepn process of chitinamine oligosaccharide |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1370784A CN1370784A (en) | 2002-09-25 |
| CN1128225C true CN1128225C (en) | 2003-11-19 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 01107822 Expired - Fee Related CN1128225C (en) | 2001-02-24 | 2001-02-24 | Prepn process of chitinamine oligosaccharide |
Country Status (1)
| Country | Link |
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| CN (1) | CN1128225C (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101455397B (en) * | 2009-01-07 | 2012-01-11 | 吉林农业大学 | Animals and plants polysaccharide oligomerization method |
| BE1019290A5 (en) | 2010-04-14 | 2012-05-08 | Nutrition Sciences Nv Sa | FOOD SUPPLEMENT INCLUDING OLIGOSACCHARIDES AND MEDIUM CHAIN FATTY ACIDS. |
| CN102702386A (en) * | 2012-06-14 | 2012-10-03 | 中国科学院海洋研究所 | Method for degrading chitosan |
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2001
- 2001-02-24 CN CN 01107822 patent/CN1128225C/en not_active Expired - Fee Related
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| Publication number | Publication date |
|---|---|
| CN1370784A (en) | 2002-09-25 |
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