CN112772668B - Fly killing composition containing chlorbenzuron and application thereof - Google Patents

Abstract

The invention discloses a fly killing composition containing chlorbenzuron and application thereof, wherein the active ingredients of the fly killing composition are chlorbenzuron and dinotefuran/acetamiprid, and the weight ratio of the chlorbenzuron to the dinotefuran is 1: 15-15: 1, preferably 1: 10-8: 1; the weight ratio of chlorbenzuron to acetamiprid is 1: 10-10: 1, preferably 1: 8-5: 1. the invention also provides missible oil consisting of the fly killing composition, which consists of 10-50 percent of active ingredients, 1-15 percent of emulsifier, 1-10 percent of penetrant and the balance of solvent. The pesticide is used for controlling flies breeding by compounding chlorbenzuron and dinotefuran or acetamiprid. The composition has good permeability, good lethal effect, long persistent period, high emergence inhibition rate and reduced labor cost; the insecticidal composition is widely applied to places such as garbage piles, manure pits, farms and the like, has special effects on controlling bred ground fly larvae, and simultaneously kills adults.

Description

Fly killing composition containing chlorbenzuron and application thereof

Technical Field

The invention relates to a sanitary insecticide, in particular to a compound sanitary insecticide containing chlorbenzuron and dinotefuran or acetamiprid.

Background

Flies are common sanitary pests and mainly cause mechanical transmission diseases, harassment blood absorption and other hazards. The habitat of the fly larvae is generally called a breeding place, and breeding substances are basic conditions for breeding the flies. A great deal of waste such as food residue, excrement, production leftovers, animal carcasses and the like generated in the daily life and production process of human beings can be used as a living substrate for breeding the fly larvae.

The cleaning of the breeding ground is the most direct and effective means for controlling fly larvae, and chemical control means are needed when the breeding ground is not treated well. The fly larvae are favored to be hidden in deep parts of breeding grounds, how to enable the medicament to penetrate deep into the breeding grounds is one of the influencing factors influencing the effect of the fly larvae of the breeding grounds, and most of the current chemical control methods are granules, aqueous emulsion and microemulsion, which have not good effect on controlling the fly larvae in the deep parts of the breeding grounds. Moreover, the single use of a single drug can induce drug resistance.

Chlorbenzuron is an insect growth regulator, belongs to a specific insecticide, and has the insecticidal mechanism that the synthesis of chitin in an insect body is inhibited, so that the insect can not normally molt, and the insect is killed. Mainly takes stomach toxicity as main action and takes contact killing action as secondary action, has slow drug effect speed, longer residual effect period and rain wash resistance, is nontoxic to natural enemies such as birds, fishes, bees and the like, and does not destroy ecological balance.

Dinotefuran is a new generation of super nicotine pesticide, and has the characteristics of contact poisoning, stomach toxicity, systemic property on roots, high quick effect, wide insecticidal spectrum and the like; the acetamiprid belongs to pyridine compounds, has strong osmosis effect besides contact poisoning and stomach poisoning effects, and shows quick-acting insecticidal activity.

The chlorbenzuron, the dinotefuran and the acetamiprid have different action mechanisms, the chlorbenzuron has poor quick-acting property but long lasting effect, and the pest drug resistance is easy to occur when one medicament is singly used, so that the pesticide application cost is increased, and the hidden danger is brought to the living environment; on the contrary, dinotefuran and acetamiprid are novel insecticide agents and have good quick action. According to the invention, the chlorbenzuron and the dinotefuran or the acetamiprid are compounded, so that the prevention and control effect of the fly larvae can be improved, and the generation of drug resistance can be slowed down.

Disclosure of Invention

The invention aims to solve the technical problem of the prior art, and provides a fly-killing composition containing chlorbenzuron, which is widely applied to places such as garbage piles, manure pits, farms and the like, has special effect on controlling the bred ground fly larvae, and simultaneously kills adults. The fly-killing composition has good insecticidal effect, is compounded with synergy by double mechanisms, and is not easy to generate drug resistance.

In order to achieve the purpose, the invention adopts the following technical scheme:

the invention firstly provides a fly killing composition containing chlorbenzuron, which is compounded by chlorbenzuron and dinotefuran/acetamiprid, wherein the weight ratio of the chlorbenzuron to the dinotefuran is 1: 15-15: 1, the weight ratio of chlorbenzuron to acetamiprid is 1: 10-10: 1.

in the above technical scheme, the weight ratio of chlorbenzuron to dinotefuran is preferably 1: 10-8: 1.

in the above technical scheme, the weight ratio of the chlorbenzuron to the acetamiprid is preferably 1: 8-5: 1.

the invention also provides a fly killing preparation containing chlorbenzuron, the formulation is missible oil, and the fly killing preparation is prepared by taking the fly killing composition, an emulsifier, a penetrating agent and a solvent as raw materials, and the fly killing preparation accounts for the total weight of the missible oil preparation in percentage by weight: 10 to 50 percent of fly killing composition, 1 to 15 percent of emulsifier, 1 to 10 percent of penetrating agent and solvent are complemented to 100 percent.

In the above technical scheme, the emulsifier is selected from: styryl phenol polyoxyethylene ether, phenyl phenol polyoxyethylene ether phosphate, castor oil polyoxyethylene ether phosphate, calcium alkyl benzene sulfonate, alkylphenol polyoxyethylene ether, phenethyl phenol formaldehyde resin polyoxyethylene ether, nonylphenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylaryl polyoxypropylene polyoxyethylene ether, alkylaryl polyoxyethylene ether, tween series and span series, and any one, two or more than two of the above are mixed in any proportion to form a mixture.

In the above technical scheme, the penetrating agent is selected from: the mixture is prepared by mixing any one or two or more of diisooctyl sulfosuccinate sodium salt, di-sec-octyl sulfosuccinate sodium salt, sodium ricinoleate sulfate, octanol polyoxyethylene ether and sodium alkyl benzene sulfonate in any proportion.

In the above technical scheme, the solvent is selected from: any one, two or more than two of dimethyl sulfoxide, N-dimethylformamide, N-dimethylacetamide, N-methylpyrrolidone, cyclohexanone, benzyl alcohol and N-butyl alcohol are mixed in any proportion to form a mixture.

The invention also provides the application of the fly killing preparation containing the chlorbenzuron in the aspect of preventing and controlling sanitary pests at the breeding places of flies.

In the technical scheme, the fly breeding places refer to garbage piles, manure pits, farms and the like.

In the technical scheme, the sanitary insect pests refer to larvae and/or adults of flies.

In the technical scheme, when the fly killing preparation containing the chlorbenzuron is used for preventing and controlling sanitary pests at fly breeding places, the fly killing preparation is diluted by 100-fold and 200-fold with water and then sprayed on the fly breeding places.

The technical scheme of the invention has the advantages that: the pesticide is used for controlling flies breeding by compounding chlorbenzuron and dinotefuran or acetamiprid. The composition has good permeability, good lethal effect, long persistent period, high emergence inhibition rate and reduced labor cost; the insecticidal composition is widely applied to places such as garbage piles, manure pits, farms and the like, has special effects on controlling bred ground fly larvae, and simultaneously kills adults.

Detailed Description

The following detailed description of the embodiments of the present invention is provided, but the present invention is not limited to the following descriptions:

the preparation method of the cream formulation in each embodiment of the invention is as follows: weighing chlorbenzuron, dinotefuran/acetamiprid, emulsifier, penetrant and solvent according to the proportion, and uniformly mixing in a container to obtain the pesticide.

The percentages in the following examples are by weight unless otherwise indicated.

Formulation example 1: 48% chlorbenzuron dinotefuran emulsifiable concentrate

3 percent of chlorbenzuron, 45 percent of dinotefuran, 4 percent of castor oil polyoxyethylene ether, 6 percent of calcium alkyl benzene sulfonate, 4 percent of sodium ricinoleate and 100 percent of N-methyl pyrrolidone

Formulation example 2: 33% chlorbenzuron dinotefuran emulsifiable concentrate

3% of chlorbenzuron, 30% of dinotefuran, 3% of styryl phenol polyoxyethylene ether, 7% of phenyl phenol polyoxyethylene ether phosphate, 5% of sulfonated diisooctyl succinate sodium salt and the balance of benzyl alcohol to 100%.

Formulation example 3: 24% chlorbenzuron dinotefuran emulsifiable concentrate

4% of chlorbenzuron, 20% of dinotefuran, 4% of fatty alcohol-polyoxyethylene ether, 4% of phenyl phenol polyoxyethylene ether phosphate, tween-604%, 7% of di-sec-octyl sulfosuccinate sodium salt, 8% of cyclohexanone and 100% of N, N-dimethylacetamide.

Formulation example 4: 20% chlorbenzuron dinotefuran emulsifiable concentrate

The pesticide composition comprises, by weight, 10% of chlorbenzuron, 10% of dinotefuran, 5% of calcium alkyl benzene sulfonate, 3% of fatty alcohol-polyoxyethylene ether, 2% of phenyl phenol polyoxyethylene ether phosphate, 2% of sodium alkyl benzene sulfonate, 3% of sodium ricinoleate sulfate and 100% of N-methyl pyrrolidone.

Formulation example 5: 18% chlorbenzuron dinotefuran emulsifiable concentrate

Chlorbenzuron 15%, dinotefuran 3%, nonylphenol polyoxyethylene ether 4%, alkylaryl polyoxyethylene ether 2%, castor oil polyoxyethylene ether phosphate 5%, di-sec-octyl sulfosuccinate sodium salt 10%, cyclohexanone 5%, and N, N-dimethylacetamide to make up to 100%

Formulation example 6: 33% chlorbenzuron dinotefuran emulsifiable concentrate

30% of chlorbenzuron, 3% of dinotefuran, 5% of calcium alkyl benzene sulfonate, 4% of fatty alcohol-polyoxyethylene ether, 5% of alkylaryl polyoxypropylene-polyoxyethylene ether, 6% of sodium ricinoleate sulfate and 100% of dimethyl sulfoxide.

Formulation example 7: 40% chlorbenzuron dinotefuran emulsifiable concentrate

37.5% of chlorbenzuron, 2.5% of dinotefuran, 4% of castor oil polyoxyethylene ether phosphate, 6% of fatty alcohol polyoxyethylene ether, tween-602%, 7% of octanol polyoxyethylene ether, 6% of N-butanol and N, N-dimethylformamide to make up to 100%.

Formulation example 8: 44% chlorbenzuron and acetamiprid emulsifiable concentrate

4% of chlorbenzuron, 40% of acetamiprid, 3% of styrylphenol polyoxyethylene ether, 7% of alkylaryl polyoxypropylene polyoxyethylene ether, 2% of alkylphenol polyoxyethylene ether, 9% of diisooctyl sulfosuccinate sodium salt and 100% of N-methyl pyrrolidone.

Formulation example 9: 30% chlorbenzuron and acetamiprid emulsifiable concentrate

5% of chlorbenzuron, 25% of acetamiprid, 4% of castor oil polyoxyethylene ether, 6% of alkylphenol polyoxyethylene ether, 5% of span-803%, 5% of ricinoleic acid sodium sulfate, 10% of benzyl alcohol and 100% of N, N-dimethylacetamide.

Formulation example 10: 20% chlorbenzuron and acetamiprid emulsifiable concentrate

The pesticide composition comprises, by weight, 10% of chlorbenzuron, 10% of acetamiprid, 3% of castor oil polyoxyethylene ether, 5% of alkylphenol polyoxyethylene ether, 2% of octanol polyoxyethylene ether, 5% of ricinoleic acid sodium sulfate, 9% of cyclohexanone and 100% of N, N-dimethylacetamide.

Formulation example 11: 18% chlorbenzuron and acetamiprid emulsifiable concentrate

15% of chlorbenzuron, 3% of acetamiprid, 4% of castor oil polyoxyethylene ether, 4% of alkylaryl polyoxyethylene ether, 3% of ricinoleic acid sodium sulfate, 3% of sodium alkyl benzene sulfonate and 100% of dimethyl sulfoxide.

Formulation example 12: 22% chlorbenzuron and acetamiprid emulsifiable concentrate

20% of chlorbenzuron, 2% of acetamiprid, 4% of fatty alcohol-polyoxyethylene ether, 5% of calcium alkyl benzene sulfonate, 4% of di-sec-octyl sulfosuccinate sodium salt and 100% of N, N-dimethylformamide.

Application example 1: drug effect test for controlling fly larvae

Test targets: larva of 3-instar housefly

The test method comprises the following steps: laying housefly larva fodder with thickness of 5cm in a wooden box with thickness of 20cm × 50cm × 10cm, placing 100 housefly larvae, applying the agent on the surface of the fodder, spraying 50g of liquid medicine, covering the box with gauze, observing survival insect number after placing for 10 days, calculating out emergence inhibition rate, and setting a blank control group;

and (3) test treatment: preparation examples 1-3,8-10 total concentration of active ingredients were diluted to 500 mg/l;

control 1: diluting 5% chlorbenzuron emulsifiable concentrate to 500 mg/l;

control 2: diluting 20% dinotefuran emulsifiable solution to 500 mg/l;

control 3: diluting 20% acetamiprid missible oil to 500 mg/l;

blank control: distilled water

And (3) test results:

the test result shows that: the control 1-3 are single-agent emulsifiable concentrates, the feathering inhibition rate of the fly larvae is lower than that of the preparation of the invention, and the preparation examples 1-3,8-10 of the invention have good lethal effect and high feathering inhibition rate (see Table 1)

TABLE 1 formulation examples 1-3,8-10 efficacy tests for fly larvae control

Further, the same efficacy test is carried out on the preparation examples 4-7 and 11-12, the lethal effect is good, the eclosion rate is very high, and the persistence is good.

Application example 2: simulated field test for fly larvae control

Test targets: larva of 3-instar housefly

The test method comprises the following steps: laying 20cm thick housefly larva feed in a 20cm x 50cm x 30cm wooden box, applying a medicament on the surface of the feed, spraying 50g of liquid medicine, then putting 100 3-instar housefly larvae into the wooden box after the day, 2 weeks and 4 weeks, covering the box with gauze, observing the number of surviving imagoes after 10 days, calculating the emergence inhibition rate, and properly spraying water after 2 weeks and 4 weeks of the test to keep the feed moist;

and (3) test treatment: preparation examples 4-5,11-12 total concentration of active ingredients were diluted to 500 mg/l;

control 1: diluting 5% chlorbenzuron emulsifiable concentrate to 500 mg/l;

control 2: diluting 20% dinotefuran emulsifiable solution to 500 mg/l;

control 3: diluting 20% acetamiprid missible oil to 500 mg/l;

and (3) test results:

the test result shows that: the comparison samples 1-3 are single-agent missible oil, the feathering inhibition rate of the preparation is lower than that of the preparation, and the persistence is lower than that of the preparation. In the preparation examples 4-5 and 11-12 of the present invention, not only the lethal effect is good, but also the emergence retarding rate is high, and the persistence is good (see Table 2)

Table 2 formulation examples 4-5,11-12 simulated field trials for fly larvae control

Further, the same efficacy tests are carried out on the preparation examples 1-3 and 6-10, the lethal effect is good, the eclosion rate is very high, and the persistence is good.

Application example 3: permeability test of larvae of Drosophila infestans

Test targets: larva of 3-instar housefly

The test method comprises the following steps: laying housefly larva feed with thickness of 10cm, 20cm and 30cm in a wooden box with thickness of 20cm, 50cm and 30cm, applying the medicament on the surface of the feed, spraying 50g of liquid medicine, then putting 100 housefly larvae with age of 3 into the wooden box, covering the wooden box with gauze, observing the number of survival imagoes after 10 days, and calculating the eclosion inhibition rate;

and (3) test treatment: formulation examples 2 and 9 the total concentration of active ingredient was diluted to 500 mg/l;

control 1: the penetrant was removed following the formulation example 2;

control 2: the penetrant is removed following the formulation example 9;

and (3) test results:

the test results show that the control samples 1 and 2, without penetrant, have decreasing emergence inhibition rates for fly larvae with increasing feed thickness, and have lower emergence inhibition rates than the preparation examples 2 and 9, and have poorer permeability performance than the preparation examples 2 and 9, the preparation examples 2 and 9 of the invention have good feed penetration effects and high emergence inhibition rates with different thicknesses (see table 3)

TABLE 3 Drosophila larvae Permeability pharmacodynamic test

Application example 4: drug effect test of different formulations on bred ground fly larvae

Test targets: larva of 3-instar housefly

The test method comprises the following steps: laying housefly larva feed with the thickness of 20cm in a wooden box with the thickness of 20cm multiplied by 50cm multiplied by 30cm, applying a medicament on the surface of the feed, spraying 50g of liquid medicine, then putting 100 housefly larvae with the age of 3 into the wooden box, covering the wooden box with gauze, observing the number of survival imagoes after 10 days, and calculating the emergence rate of the imagoes blocked;

and (3) test treatment: formulation examples 3 and 10 the total concentration of active ingredient was diluted to 500 mg/l;

control 1: preparation example 3 the microemulsion of chlorbenzuron and dinotefuran with the same raw pesticide ratio is diluted to 500 mg/l;

control 2: preparation example 3 the same raw material ratio of chlorbenzuron and dinotefuran aqueous emulsion is diluted to 500 mg/l;

control 3: in the preparation example 10, the micro-emulsion of chlorbenzuron and acetamiprid with the same raw pesticide ratio is diluted to 500 mg/l;

control 4: preparation example 10 the same raw material ratio of chlorbenzuron and acetamiprid aqueous emulsion is diluted to 500 mg/l;

and (3) test results:

the test results showed that the control samples 1-2 had a lower feathering inhibition ratio for fly larvae than that of the formulation example 3, and had poorer penetration properties than that of the formulation example 3, and the control samples 3-4 had a lower feathering inhibition ratio for fly larvae than that of the formulation example 10, and had poorer penetration properties than that of the formulation example 10. The formulations of examples 3 and 10 according to the invention have good penetration and a high emergence inhibition (see Table 4)

TABLE 4 drug effect test of different formulations on bred Dilophaga terrestris

Application example 5: outdoor efficacy test of adult ground flies bred

Test targets: adult fly

The test method comprises the following steps: spraying or scattering a medicament on the surface of a breeding ground in a garbage field subarea, and recording the density reduction rate of the fly imagoes for 24 hours;

and (3) test treatment: preparation example 4, preparation example 11 total concentration of active ingredients is diluted to 500 mg/l;

and (3) test results:

the test result shows that the preparation of the invention has the effect of killing imagoes and high fly density reduction rate (see table 5)

TABLE 5 outdoor efficacy test of adult ground flies bred

Treatment of	Density decrease ratio (%)
		Formulation example 4	96.9
Formulation example 11	97.2

Application example 6: determination of co-toxicity coefficient of chlorbenzuron and dinotefuran or acetamiprid to fly larvae

Test targets: larva of 3-instar housefly

The test method comprises the following steps: the method comprises the steps of filling 30g of fly feed in a white enamel bowl with the volume of 300mL, preparing solutions with different concentrations, sucking 40mL of the fly feed, mixing the fly feed with the solutions, standing for several minutes, putting 50 housefly larvae of 3 years old into the bowl, observing the number of the housefly larvae which survive for 10 days, considering all pupae which cannot be eclosized as dead, and calculating the death rate;

the data calculates half lethal concentration value and virulence regression equation according to linear weighted regression method, and calculates cotoxicity coefficient by referring to Sun cloud Pepper method.

The test results show that the insecticidal composition has good synergistic effect on the feathering inhibition rate of the fly larvae after being compounded through indoor pesticide effect tests and calculation, and the specific results are shown in tables 6 and 7:

TABLE 6 chlorbenzuron and dinotefuran cotoxicity coefficient determination (for housefly larvae)

As shown in table 6, the compounding ratio of chlorbenzuron and dinotefuran is 1: 15-15: 1, the co-toxicity coefficient for fly larvae is more than 100, and particularly, when the compounding ratio is 1: 10-8: when 1, the co-toxicity coefficient exceeds 120, and the synergistic effect is obvious.

TABLE 7 measurement of Co-toxicity coefficients of chlorbenzuron and acetamiprid (for housefly larvae)

As shown in table 7, the compounding ratio of chlorbenzuron and acetamiprid is 1: 10-10: 1, the co-toxicity coefficient for fly larvae is more than 100, and particularly, when the compounding ratio is 1: 8-5: when 1, the co-toxicity coefficient exceeds 120, and the synergistic effect is obvious.

The above examples are only for illustrating the technical concept and features of the present invention, and are not intended to limit the scope of the present invention. All equivalent changes or modifications made according to the spirit of the present invention should be covered within the protection scope of the present invention.

Claims (6)

1. The application of the fly killing composition containing the chlorbenzuron in the aspect of preventing and controlling sanitary pests at the breeding places of flies is characterized in that the fly killing composition is prepared by compounding the chlorbenzuron and the dinotefuran/acetamiprid, and the weight ratio of the chlorbenzuron to the dinotefuran is 1: 10-8: 1; the weight ratio of chlorbenzuron to acetamiprid is 1: 8-5: 1;

the breeding places of the flies refer to garbage piles, manure pits and farms; the sanitary insect is larva of fly.

2. The use as claimed in claim 1, wherein the formulation of the said fly-killing composition is an emulsifiable concentrate, which is prepared from the fly-killing composition containing chlorbenzuron and emulsifier, penetrant, solvent as raw materials, and the percentages of each in the total weight of the emulsifiable concentrate formulation are: 10 to 50 percent of fly killing composition, 1 to 15 percent of emulsifier, 1 to 10 percent of penetrating agent and solvent are complemented to 100 percent.

3. Use according to claim 2, wherein the emulsifier is selected from the group consisting of: styryl phenol polyoxyethylene ether, phenyl phenol polyoxyethylene ether phosphate, castor oil polyoxyethylene ether phosphate, calcium alkyl benzene sulfonate, alkylphenol polyoxyethylene ether, phenethyl phenol formaldehyde resin polyoxyethylene ether, nonylphenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylaryl polyoxypropylene polyoxyethylene ether, alkylaryl polyoxyethylene ether, tween series and span series, and any one, two or more than two of the above are mixed in any proportion to form a mixture.

4. The use according to claim 2, wherein the osmotic agent is selected from the group consisting of: the mixture is prepared by mixing any one or two or more of diisooctyl sulfosuccinate sodium salt, di-sec-octyl sulfosuccinate sodium salt, sodium ricinoleate sulfate, octanol polyoxyethylene ether and sodium alkyl benzene sulfonate in any proportion.

5. The use according to claim 2, wherein the solvent is selected from the group consisting of: any one, two or more than two of dimethyl sulfoxide, N-dimethylformamide, N-dimethylacetamide, N-methylpyrrolidone, cyclohexanone, benzyl alcohol and N-butyl alcohol are mixed in any proportion to form a mixture.

6. Use according to claim 1, characterized in that: when the fly killing composition containing the chlorbenzuron is used for preventing and controlling sanitary pests in fly breeding places, the fly killing composition is diluted by 100-fold and 200-fold with water and then sprayed on the fly breeding places.