CN112735525A - 一种基于分治法的mRNA序列优化的方法与装置 - Google Patents
一种基于分治法的mRNA序列优化的方法与装置 Download PDFInfo
- Publication number
- CN112735525A CN112735525A CN202110039347.7A CN202110039347A CN112735525A CN 112735525 A CN112735525 A CN 112735525A CN 202110039347 A CN202110039347 A CN 202110039347A CN 112735525 A CN112735525 A CN 112735525A
- Authority
- CN
- China
- Prior art keywords
- codon
- codons
- mrna sequence
- optimized
- protein expression
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108020004999 messenger RNA Proteins 0.000 title claims abstract description 100
- 238000000034 method Methods 0.000 title claims abstract description 69
- 238000005457 optimization Methods 0.000 title claims abstract description 37
- 230000014509 gene expression Effects 0.000 claims abstract description 67
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 64
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 55
- 108020004705 Codon Proteins 0.000 claims description 148
- 230000006978 adaptation Effects 0.000 claims description 12
- 230000008569 process Effects 0.000 claims description 8
- 108020005350 Initiator Codon Proteins 0.000 claims description 7
- 108700026244 Open Reading Frames Proteins 0.000 claims description 7
- 108020005038 Terminator Codon Proteins 0.000 claims description 7
- 238000005215 recombination Methods 0.000 claims description 7
- 230000006798 recombination Effects 0.000 claims description 7
- 108091008146 restriction endonucleases Proteins 0.000 claims description 7
- 108090000589 ribonuclease E Proteins 0.000 claims description 7
- 239000012634 fragment Substances 0.000 claims description 6
- 108700026226 TATA Box Proteins 0.000 claims description 5
- 238000004364 calculation method Methods 0.000 claims description 5
- 238000011176 pooling Methods 0.000 claims description 4
- 238000012935 Averaging Methods 0.000 claims description 3
- 230000015572 biosynthetic process Effects 0.000 claims description 3
- 239000013612 plasmid Substances 0.000 description 13
- 238000004422 calculation algorithm Methods 0.000 description 10
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 9
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 8
- 230000006872 improvement Effects 0.000 description 8
- 238000010586 diagram Methods 0.000 description 7
- 238000001976 enzyme digestion Methods 0.000 description 7
- 238000000338 in vitro Methods 0.000 description 7
- 238000013518 transcription Methods 0.000 description 7
- 230000035897 transcription Effects 0.000 description 7
- 238000000246 agarose gel electrophoresis Methods 0.000 description 6
- 241000894007 species Species 0.000 description 6
- 108700010070 Codon Usage Proteins 0.000 description 5
- 238000004590 computer program Methods 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 238000003776 cleavage reaction Methods 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 238000000746 purification Methods 0.000 description 4
- 230000007017 scission Effects 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- 238000001890 transfection Methods 0.000 description 3
- 108091026890 Coding region Proteins 0.000 description 2
- 108020004414 DNA Proteins 0.000 description 2
- 241000206602 Eukaryota Species 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- 108091034057 RNA (poly(A)) Proteins 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 238000011068 loading method Methods 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 230000005026 transcription initiation Effects 0.000 description 2
- 238000013519 translation Methods 0.000 description 2
- 108020003589 5' Untranslated Regions Proteins 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 239000013614 RNA sample Substances 0.000 description 1
- 230000006819 RNA synthesis Effects 0.000 description 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
- 108010083644 Ribonucleases Proteins 0.000 description 1
- 102000006382 Ribonucleases Human genes 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 108700005078 Synthetic Genes Proteins 0.000 description 1
- 108020004566 Transfer RNA Proteins 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 125000003275 alpha amino acid group Chemical group 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000007622 bioinformatic analysis Methods 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000001687 destabilization Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 239000002158 endotoxin Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- 238000002073 fluorescence micrograph Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000003517 fume Substances 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- JGBUYEVOKHLFID-UHFFFAOYSA-N gelred Chemical compound [I-].[I-].C=1C(N)=CC=C(C2=CC=C(N)C=C2[N+]=2CCCCCC(=O)NCCCOCCOCCOCCCNC(=O)CCCCC[N+]=3C4=CC(N)=CC=C4C4=CC=C(N)C=C4C=3C=3C=CC=CC=3)C=1C=2C1=CC=CC=C1 JGBUYEVOKHLFID-UHFFFAOYSA-N 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 125000001967 indiganyl group Chemical group [H][In]([H])[*] 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000012160 loading buffer Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000000424 optical density measurement Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000002028 premature Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000003259 recombinant expression Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 230000005945 translocation Effects 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 238000012070 whole genome sequencing analysis Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B50/00—ICT programming tools or database systems specially adapted for bioinformatics
- G16B50/40—Encryption of genetic data
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Theoretical Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Biotechnology (AREA)
- Evolutionary Biology (AREA)
- Medical Informatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioethics (AREA)
- Molecular Biology (AREA)
- Databases & Information Systems (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202110039347.7A CN112735525B (zh) | 2021-01-18 | 2021-01-18 | 一种基于分治法的mRNA序列优化的方法与装置 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202110039347.7A CN112735525B (zh) | 2021-01-18 | 2021-01-18 | 一种基于分治法的mRNA序列优化的方法与装置 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN112735525A true CN112735525A (zh) | 2021-04-30 |
CN112735525B CN112735525B (zh) | 2023-12-26 |
Family
ID=75592769
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202110039347.7A Active CN112735525B (zh) | 2021-01-18 | 2021-01-18 | 一种基于分治法的mRNA序列优化的方法与装置 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN112735525B (zh) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113851190A (zh) * | 2021-11-01 | 2021-12-28 | 四川大学华西医院 | 一种异种mRNA序列优化方法 |
CN114708909A (zh) * | 2022-03-21 | 2022-07-05 | 深圳市新合生物医疗科技有限公司 | mRNA序列的优化方法及装置、设备、存储介质 |
CN117497092A (zh) * | 2024-01-02 | 2024-02-02 | 合肥微观纪元数字科技有限公司 | 基于动态规划和量子退火的rna结构预测方法及系统 |
Citations (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1997048370A2 (en) * | 1996-06-21 | 1997-12-24 | Merck & Co., Inc. | Vaccines comprising synthetic genes |
WO2002098443A2 (de) * | 2001-06-05 | 2002-12-12 | Curevac Gmbh | Stabilisierte mrna mit erhöhtem g/ c- gehalt und otimierter codon usage für die gentherapie |
CN101134966A (zh) * | 1997-10-20 | 2008-03-05 | Gtc生物治疗学公司 | 修饰的核酸序列以及增加细胞系统中mRNA水平和蛋白质表达的方法 |
JP2009538131A (ja) * | 2006-05-25 | 2009-11-05 | インスティチュート フォー アドバンスド スタディ | 配列モチーフを同定するための方法、およびその応用 |
US20110081708A1 (en) * | 2009-10-07 | 2011-04-07 | Genscript Holdings (Hong Kong) Limited | Method of Sequence Optimization for Improved Recombinant Protein Expression using a Particle Swarm Optimization Algorithm |
US20130123483A1 (en) * | 2002-12-23 | 2013-05-16 | Geneart Ag | Method and device for optimizing a nucelotide sequence for the purpose of expression in a protein |
CN104673802A (zh) * | 2015-03-12 | 2015-06-03 | 山东大学第二医院 | 一种编码irisin蛋白的核酸分子以及利用该核酸分子高效表达irisin蛋白的方法 |
US20170016008A1 (en) * | 2015-07-15 | 2017-01-19 | The Penn State Research Foundation | DESIGN OF mRNA SEQUENCES TO CONTROL CO-TRANSLATIONAL FOLDING OF PROTEINS |
WO2018104385A1 (en) * | 2016-12-07 | 2018-06-14 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Codon optimization |
CN108363904A (zh) * | 2018-02-07 | 2018-08-03 | 南京林业大学 | 一种用于木本植物遗传密码子优化的CodonNX系统及其优化方法 |
WO2019020054A1 (zh) * | 2017-07-25 | 2019-01-31 | 南京金斯瑞生物科技有限公司 | 一种基于免疫算法的密码子优化方法 |
CN110491447A (zh) * | 2019-08-05 | 2019-11-22 | 浙江省农业科学院 | 一种用于异源基因体外表达的密码子优化方法及应用 |
TW202008379A (zh) * | 2018-07-30 | 2020-02-16 | 大陸商南京金斯瑞生物科技有限公司 | 密碼子優化 |
-
2021
- 2021-01-18 CN CN202110039347.7A patent/CN112735525B/zh active Active
Patent Citations (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1997048370A2 (en) * | 1996-06-21 | 1997-12-24 | Merck & Co., Inc. | Vaccines comprising synthetic genes |
CN101134966A (zh) * | 1997-10-20 | 2008-03-05 | Gtc生物治疗学公司 | 修饰的核酸序列以及增加细胞系统中mRNA水平和蛋白质表达的方法 |
WO2002098443A2 (de) * | 2001-06-05 | 2002-12-12 | Curevac Gmbh | Stabilisierte mrna mit erhöhtem g/ c- gehalt und otimierter codon usage für die gentherapie |
US20130123483A1 (en) * | 2002-12-23 | 2013-05-16 | Geneart Ag | Method and device for optimizing a nucelotide sequence for the purpose of expression in a protein |
JP2009538131A (ja) * | 2006-05-25 | 2009-11-05 | インスティチュート フォー アドバンスド スタディ | 配列モチーフを同定するための方法、およびその応用 |
US20110081708A1 (en) * | 2009-10-07 | 2011-04-07 | Genscript Holdings (Hong Kong) Limited | Method of Sequence Optimization for Improved Recombinant Protein Expression using a Particle Swarm Optimization Algorithm |
CN104673802A (zh) * | 2015-03-12 | 2015-06-03 | 山东大学第二医院 | 一种编码irisin蛋白的核酸分子以及利用该核酸分子高效表达irisin蛋白的方法 |
US20170016008A1 (en) * | 2015-07-15 | 2017-01-19 | The Penn State Research Foundation | DESIGN OF mRNA SEQUENCES TO CONTROL CO-TRANSLATIONAL FOLDING OF PROTEINS |
WO2018104385A1 (en) * | 2016-12-07 | 2018-06-14 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Codon optimization |
WO2019020054A1 (zh) * | 2017-07-25 | 2019-01-31 | 南京金斯瑞生物科技有限公司 | 一种基于免疫算法的密码子优化方法 |
CN108363904A (zh) * | 2018-02-07 | 2018-08-03 | 南京林业大学 | 一种用于木本植物遗传密码子优化的CodonNX系统及其优化方法 |
TW202008379A (zh) * | 2018-07-30 | 2020-02-16 | 大陸商南京金斯瑞生物科技有限公司 | 密碼子優化 |
CN110491447A (zh) * | 2019-08-05 | 2019-11-22 | 浙江省农业科学院 | 一种用于异源基因体外表达的密码子优化方法及应用 |
Non-Patent Citations (4)
Title |
---|
KATZ, L,等: "Widespread selection for local RNA secondary structure in coding regions of bacterial genes", GENOME RESEARCH, vol. 13, no. 9, pages 2042 - 2051, XP093050903, DOI: 10.1101/gr.1257503 * |
杨云彭,等: "密码子优化策略在异源蛋白表达中的应用", 生物工程学报, no. 12, pages 2227 - 2237 * |
肖景发,等: "遗传密码的新排列和起源探讨", 中国科学(C辑:生命科学), no. 08, pages 717 - 726 * |
袁巧敏,等: "家蝇乙酰胆碱酯酶基因密码子优化及酶学特性表征", 中国食品学报, vol. 20, no. 12, pages 72 - 80 * |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113851190A (zh) * | 2021-11-01 | 2021-12-28 | 四川大学华西医院 | 一种异种mRNA序列优化方法 |
CN114708909A (zh) * | 2022-03-21 | 2022-07-05 | 深圳市新合生物医疗科技有限公司 | mRNA序列的优化方法及装置、设备、存储介质 |
WO2023179273A1 (zh) * | 2022-03-21 | 2023-09-28 | 深圳市新合生物医疗科技有限公司 | mRNA序列的优化方法及装置、设备、存储介质 |
CN114708909B (zh) * | 2022-03-21 | 2023-10-20 | 深圳市新合生物医疗科技有限公司 | mRNA序列的优化方法及装置、设备、存储介质 |
CN117497092A (zh) * | 2024-01-02 | 2024-02-02 | 合肥微观纪元数字科技有限公司 | 基于动态规划和量子退火的rna结构预测方法及系统 |
CN117497092B (zh) * | 2024-01-02 | 2024-05-14 | 微观纪元(合肥)量子科技有限公司 | 基于动态规划和量子退火的rna结构预测方法及系统 |
Also Published As
Publication number | Publication date |
---|---|
CN112735525B (zh) | 2023-12-26 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN112735525B (zh) | 一种基于分治法的mRNA序列优化的方法与装置 | |
Boldogkői et al. | Long-read sequencing–a powerful tool in viral transcriptome research | |
JP5483628B2 (ja) | 正確な配列データおよび修飾塩基位置決定の方法 | |
CN105849276B (zh) | 用于检测结构变异体的系统和方法 | |
JP7171709B2 (ja) | 圧縮分子タグ付き核酸配列データを用いた融合の検出のための方法 | |
EP2668277A1 (en) | Method for the construction of specific promoters | |
WO2016011378A1 (en) | Systems and methods for detecting structural variants | |
CN110878334B (zh) | 用于扩增子测序的引物及两步pcr建库方法 | |
CN116179512B (zh) | 靶标识别范围广的核酸内切酶及其应用 | |
US20240254465A1 (en) | Heat-resistant endonuclease and gene editing system mediated by heat-resistant endonuclease | |
CN116410955B (zh) | 两种新型核酸内切酶及其在核酸检测中的应用 | |
CN117210437A (zh) | 两种基因编辑工具酶鉴定及其在核酸检测中的应用 | |
CN115843318B (zh) | 基于全基因组分析与基因组编辑的植物物种鉴定方法与应用 | |
CN113293200B (zh) | 一种降低或消除二代测序中扩增产物污染的方法及应用 | |
US11685948B2 (en) | Method for analyzing a nucleic acid sequence | |
CN114214734A (zh) | 一种单分子靶标基因建库方法及其试剂盒 | |
CN113969311B (zh) | 一种检测基因编辑后的突变的方法 | |
CN116286991B (zh) | 全基因组增强子筛选系统、筛选方法及应用 | |
CN118599978A (zh) | 一种piRNA的分析方法 | |
Grybchuk et al. | Analysis of Leishbuviridae from Trypanosomatids | |
CN118755698A (zh) | Pam兼容性高的基因编辑核酸内切酶及其应用 | |
Gvozdenov | Genome‐Wide Mapping of 5′ Isoforms with 5′‐Seq | |
CN118345153A (zh) | 基于ONT测序的动植物转录组poly(A)的测定分析方法 | |
WO2024174032A1 (en) | Sequence analysis of manufactured genetic constructs | |
Kahraman | Evaluation of blood-based microRNAs toward clinical use as biomarkers in common and rare diseases |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
TA01 | Transfer of patent application right | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20220208 Address after: 215000 unit 201, building 23, Tengfei Science Park, No. 388, Xinping street, Suzhou Industrial Park, Suzhou area, China (Jiangsu) pilot Free Trade Zone, Suzhou, Jiangsu Province Applicant after: Suzhou Kerui Maide Biomedical Technology Co.,Ltd. Address before: 215000 units 01, 2 / F and 05 & 06 & 07 & 08 & 09 & 10, 1 / F, building 23, Tengfei Science Park, No. 388, Xinping street, Suzhou Industrial Park, Jiangsu Province Applicant before: JIANGSU PURECELL BIO MEDICINE TECHNOLOGY Co.,Ltd. |
|
EE01 | Entry into force of recordation of patent licensing contract | ||
EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20210430 Assignee: Shanghai Shenruilian Biopharmaceutical Co.,Ltd. Assignor: Suzhou Kerui Maide Biomedical Technology Co.,Ltd. Contract record no.: X2023310000140 Denomination of invention: A Method and Device for Optimizing mRNA Sequences Based on Divide and Conquer Method License type: Exclusive License Record date: 20230731 |
|
GR01 | Patent grant | ||
GR01 | Patent grant |