CN112522214B - High-lytic pseudomonas aeruginosa bacteriophage RDP-PA-20001 and application thereof - Google Patents

Abstract

The invention discloses a high-lytic pseudomonas aeruginosa bacteriophage RDP-PA-20001 and application thereof in pathogenic pseudomonas aeruginosa infection of poultry. The invention utilizes a double-layer plate method to separate a phage from excrement of a Qingdao broiler farm, namely inky chicken farm, which is named as pseudomonas aeruginosa phage RDP-PA-20001 and the preservation number is CGMCC No. 21410. The pseudomonas aeruginosa bacteriophage RDP-PA-20001 provided by the invention has higher temperature tolerance and wider acid-base tolerance range, and is beneficial to industrial production and preparation; the phage has strong lytic effect on pseudomonas aeruginosa BP-20001, can effectively prevent and control the generation and the propagation of pseudomonas aeruginosa, and can be used for efficiently and reliably treating poultry diseases caused by pseudomonas aeruginosa.

Description

High-lytic pseudomonas aeruginosa bacteriophage RDP-PA-20001 and application thereof

Technical Field

The invention relates to the field of biotechnology, in particular to a high-lytic pseudomonas aeruginosa bacteriophage RDP-PA-20001 and application thereof in infection of pseudomonas aeruginosa of poultry animals.

Background

Pseudomonas aeruginosa is a common gram-negative bacillus, referred to as pseudomonas aeruginosa for short, is a bacterium with very strong adaptability, exists in the natural environment, also exists in the skin, intestinal tract and respiratory tract of a human body, mainly causes nosocomial infection, and often causes infection of the respiratory tract, urinary system and burn wound. The bacterium can not be detected in the cosmetic hygiene standard, and the pseudomonas aeruginosa polluted by swimming pool water can also cause the upper respiratory tract infection of people. Food poisoning is caused by eating food contaminated by pseudomonas aeruginosa, and the food-borne pseudomonas aeruginosa contamination becomes one of the risk factors harming human health. The pseudomonas aeruginosa has an asymptomatic bacteria-carrying phenomenon in poultry bodies, can cause septicemic diseases of the poultry, has extremely high morbidity and mortality, poultry meat products carrying the pseudomonas aeruginosa are potential hazard sources, and once the pseudomonas aeruginosa in food is propagated in large quantities, toxin factors generated by the pseudomonas aeruginosa will certainly harm the health of people who eat the food.

The bacteriophage is a virus taking bacteria as a host, has no cell structure, must be copied and propagated depending on the host, is widely existed in the natural world, has strong specificity and self-lysis capability, has the functions of lysing the bacteria and controlling the growth of the bacteria, can adjust the growth and the decay of the bacteria in the natural world, takes the pseudomonas aeruginosa bacteriophage as a biological bactericide to prevent, treat and control pseudomonas aeruginosa bacteriosis in the poultry breeding process, has the advantages of low pollution and high efficiency, and reduces the influence of artificial activities on the natural world to a certain extent. Therefore, the natural enemy phage of the bacteria is utilized to solve the biological control approach with wide prospect for the bacteria.

The invention provides a safe and reliable lytic pseudomonas aeruginosa bacteriophage for treating avian pseudomonas aeruginosa, which is used for solving the problems of bacterial drug resistance and antibiotic abuse in order of comprehensive banning of corresponding countries.

Disclosure of Invention

The invention aims to provide a high-lytic pseudomonas aeruginosa bacteriophage RDP-PA-20001, and aims to solve the problems that poultry in a farm are not treated timely due to acute morbidity of infection with pseudomonas aeruginosa, drug taking is ineffective due to drug resistance of pathogenic bacteria and the like.

In order to achieve the purpose, the invention adopts the following technical scheme:

a high lytic pseudomonas aeruginosa bacteriophage RDP-PA-20001, observed by an electron microscope, has a icosahedron head, is three-dimensionally symmetrical, has a head length of 60.865nm and a tail length of 183.254nm, and is a long-tail bacteriophage family of the order of tailed bacteriophages; the gene has the full length of 92178bp and has no virulence gene and lysogen gene. The phage is preserved in China general microbiological culture Collection center (CGMCC) at 12 months and 17 days in 2020, and the preservation addresses are as follows: no. 3 Xilu No. 1 Beijing, Chaoyang, and the preservation number is CGMCC No. 21410.

Furthermore, the bacteriophage is a virulent bacteriophage, has strong lytic property to pseudomonas aeruginosa, has a lysis rate of 98 percent, and provides a bacteriophage source for industrialized, large-scale and programmed production of the bacteriophage and treatment of poultry breeding animal bacterial diseases caused by the pseudomonas aeruginosa.

Further, the bacteriophage RDP-PA-20001 acts for 1 hour at a pH ranging from 7 to 8 and its titer is 2.3X 10⁷fu/mL, suitable for growth in a neutral environment.

Further, the titer reached 10 at pH 12 for bacteriophage RDP-PA-20001²pfu/mL, at pH 4, still survived the phage, with a broad range of acid-base tolerance of the phage.

Further, the optimum temperature of the bacteriophage RDP-PA-20001 is 37 ℃, and the titer reaches 10⁹pfu/mL, the phage can be stored for a long time at-80 ℃, and the titer is maintained to be stable; acting at 80 deg.C for 30min, and maintaining titer at 10⁶pfu/mL, still having biological activity.

Further, the bacteriophage RDP-PA-20001 efficiently infects Pseudomonas aeruginosa, and the optimal multiplicity of infection is 0.1.

The invention also provides application of the high-lytic pseudomonas aeruginosa bacteriophage RDP-PA-20001 in preparation of an anti-pseudomonas aeruginosa preparation.

Another object of the present invention is to provide a bactericidal composition for the control of Pseudomonas aeruginosa, comprising an effective amount of Pseudomonas aeruginosa bacteriophage RDP-PA-20001; after the bacteriophage RDP-PA-20001 is added into the bactericidal composition, the bactericidal composition has obvious cracking effect within 1 hour. Preferably, the antibiotic for resisting pseudomonas aeruginosa is also included.

The invention provides a cleaning agent or a disinfectant, which comprises the pseudomonas aeruginosa bacteriophage RDP-PA-20001.

The invention has the beneficial effects that:

(1) the invention obtains a strain of pseudomonas aeruginosa BP-20001 by screening from excrement of a Qingdao, i.e. a Mexico-broiler farm, and obtains a bacteriophage RDP-PA-20001 by taking the pathogenic pseudomonas aeruginosa BP-20001 as a host for separation, wherein the bacteriophage RDP-PA-20001 has strong cracking effect on the pathogenic pseudomonas aeruginosa BP-20001 in a poultry breeding environment, and provides a bacteriophage source for the industrial production of the bacteriophage for the prevention and treatment of the pathogenic pseudomonas aeruginosa BP-20001 in the poultry breeding environment;

(2) the pseudomonas aeruginosa bacteriophage RDP-PA-20001 provided by the invention has an obvious lysis effect within 1 hour after the bacteriophage RDP-PA-20001 is added into a pseudomonas aeruginosa culture, has strong lysis activity and shows good application prospect;

(3) the pseudomonas aeruginosa bacteriophage RDP-PA-20001 provided by the invention acts for 30min at the temperature of 80 ℃, and the titer is maintained at 10⁶pfu/mL, still having biological activity; at pH 12, the potency can reach 10²pfu/mL, at pH 4, still phage survived; the high temperature tolerance and the wide acid-base tolerance range are shown, the titer is high, and the safety and the effectiveness are realized;

(4) the pseudomonas aeruginosa bacteriophage RDP-PA-20001 provided by the invention has good application prospect in prevention and control of poultry pseudomonas aeruginosa.

Drawings

The above and other features, properties and advantages of the present invention will become more apparent from the following description of the embodiments with reference to the accompanying drawings:

FIG. 1 is a schematic view of plaques obtained in example 2 of the present invention.

FIG. 2 is a morphological diagram of Pseudomonas aeruginosa bacteriophage RDP-PA-20001 under a transmission electron microscope;

FIG. 3 is a graph showing the effect of the bacteriophage RDP-PA-20001 of the present invention on the growth curve of Pseudomonas aeruginosa BP-20001;

FIG. 4 is a schematic diagram of the acid-base tolerance experiment of bacteriophage RDP-PA-20001 of the present invention;

FIG. 5 is a schematic diagram of the experiment on the thermal stability of the bacteriophage RDP-PA-20001 according to the present invention;

Detailed Description

The invention is further illustrated by the following figures and examples. The following examples will assist those skilled in the art in further understanding the invention, but are not intended to limit the invention in any way. It should be noted that variations and modifications can be made by persons skilled in the art without departing from the spirit of the invention. All falling within the scope of the present invention.

The invention uses pseudomonas aeruginosa BP-20001 separated from excrement of Qingdao, i.e. black broiler farm, and determines pathogenicity and drug resistance of BP-20001 through experiments, uses BP-20001 as a host, separates a pseudomonas aeruginosa bacteriophage RDP-PA-20001 from the environment, and determines the genotype of RDP-PA-20001 through a whole genome sequence.

Example 1 isolation and characterization of Pseudomonas aeruginosa BP-20001

Sampling the excrements of the chicken farm with the pseudomonas aeruginosa bacterial diseases, adopting a method of aseptic operation, carrying out 1:10 dilution by sterilized LB broth, taking 10ml of 1:10 sample dilution, adding the sample dilution into 90ml of common broth culture medium, and culturing for 18-24 hours at 37 ℃. A layer of thin bacterial film grows on the surface of the culture solution, and the culture solution is yellow green.

Selecting a culture at a thin bacterial membrane of the enrichment fluid, streaking and inoculating the culture on a cetyl trimethyl ammonium bromide agar plate, and culturing at 37 ℃ for 18-24 hours to obtain a gray-white, flat and surface-wet colony with yellow-green peripheral agar and a diameter of about 2-4 mm. Typical colonies were picked and streaked three times on a cetyltrimethylammonium bromide agar plate, and then single colonies were picked and inoculated into 5ml of LB broth liquid medium, cultured at 37 ℃ for 8h with shaking at 200rpm, to give a uniform turbid bacterial suspension. Determining the pseudomonas aeruginosa through 16sRNA molecular identification, and naming one strain of the pseudomonas aeruginosa as BP-20001; and the pathogenicity and drug resistance of BP-20001 are determined through experiments.

Example 2 isolation and identification of Pseudomonas aeruginosa bacteriophage RDP-PA-20001

The experimental excrement sample is collected from an excrement urinal in a Qingdao, i.e. Memo-broiler farm in 2020, and is used as a phage separation sample.

(1) Isolation of phage RDP-PA-20001:

taking 10 mg of feces, soaking in 10ml of LB broth overnight at 4 deg.C, centrifuging at 10000rpm for 5min, and filtering the supernatant with 0.22 μm microporous membrane for sterilization. 3ml of the filtrate and 3ml of the culture of the host bacterium in the logarithmic growth phase were taken and added together to 20ml of autoclaved LB broth, which was left to stand in an incubator at 37 ℃ overnight for the propagation of the phage which may be present. Taking 3ml of culture solution, centrifuging at 10000rpm for 5min, and filtering the supernatant with 0.22 μm microporous membrane for sterilization. The filtrate was assumed to contain phage. The culture medium is diluted with LB broth in a gradient manner, and is cultured with a host proliferation solution in logarithmic growth phase for 19-23h at 37 ℃. If the transparent plaques appear on the double-layer plate, the existence of the phage is proved, and if the plaques do not appear, the existence of no phage is proved, and the rescreening is needed.

(2) Purification of bacteriophages

The phage obtained by separation is not a kind of phage, so further purification is needed, bright, smooth and small plaques are picked from a double-plate with obvious single plaques, the plaques are placed in physiological saline, a pipetting gun is used for repeatedly blowing and beating, a 0.22 mu m microporous membrane is used for filtration and sterilization, LB broth is used for gradient dilution, and a gradient preparation double-plate capable of obtaining single plaques is selected. Repeating the steps for 4-5 times until the obtained plaques on the double-plate have the same size, shape and definition, and finishing the phage purification. The results are shown in FIG. 1, which show that the plaques are consistent in morphology, size and definition and are typical lytic phages.

(3) Determination of phage titer

Single plaques of the purified phage were picked and added to LB broth, together with the culture of the host bacterium in logarithmic growth phase, and cultured at 37 ℃ for 6 hours with shaking at 170 rpm. Centrifuging phage proliferation solution 5ml at 10000rpm for 5min, collecting supernatant, and filtering with 0.22 μm microporous membrane for sterilization. 10-fold gradient dilution to 10 with LB broth⁹Get 10 times⁷、10⁸、10⁹Double-layer plates were prepared with 0.1ml of the dilution of the double dilution and 0.1ml of the host cell growth medium, and the titer of the phage was measured.

Wherein, the titer of the phage (pfu/mL) is the number of plaques multiplied by the dilution multiple multiplied by 10; the titer of the phage can reach 10 by determination⁹Pfu/mL. The phage has an optimum temperature of 37 deg.C and titer of 10⁹pfu/mL, the phage can be stored for a long time at-80 ℃, and the titer is maintained to be stable.

Example 3 morphological Observation of phages

Applying a phosphotungstic acid negative dyeing method: phage multiplication solution 20. mu.L (titer 10)⁹pfu/mL) was dropped on a paraffin plate, and a copper mesh was placed thereon for 10min and then removed to adsorb phages. Drying at room temperature for 2-3min, dyeing with 2% phosphotungstic acid water solution, sucking water after 2min, naturally drying for 5min, and observing phage morphology with a microscope. As can be seen from the electron micrograph (FIG. 2), the head of the phage RDP-PA-20001 is icosahedron, and is three-dimensionally symmetrical, the head is 60.865nm long, the tail is 183.254nm long, and the phage belongs to the long-tail phage family of the order of tailed phage according to the latest classification and naming of the phage.

Example 4 phage genome sequencing

After enrichment culture of a single phage, centrifuging for 15 minutes at 8000g under the condition of 4 ℃, adding 10% PEG8000 and 0.5M NaCl, standing overnight, adding equal amount of chloroform, mixing uniformly, standing for layering, centrifuging for 10 minutes at 5000g, removing a chloroform layer and a PEG layer, adding restriction endonuclease for digestion treatment, suspending the phage under the condition of gradient density cesium chloride, dialyzing for 3 times by TM buffer solution at the later stage, and performing whole genome sequencing by Chimona genome biological sequencing company. The whole length of the phage genome is 92178bp, and the phage genome has no virulence genes and lysogenic genes.

Example 5 optimal multiplicity of infection (MOI) assay

Phage multiplication solution 0.5ml (titer 10)⁹pfu/mL) was diluted 10-fold in LB broth, and 0.1mL of the diluted solution and 0.1mL of the host cell growth solution (10 mL) were each diluted at an MOI value of 100, 10, 1, 0.1, 0.01, 0.001⁶fu/mL) was added to 5mL of LB broth, and cultured at 37 ℃ for 8 hours with shaking at 200 rpm. Centrifuging the culture solution at 10000rpm for 5min, and measuring the titer by double-layer plate method, wherein the combination with the highest titer is the optimal infection complex number. The results are shown in Table 1.

TABLE 1

From Table 1, the optimal multiplicity of infection of RDP-PA-20001 is 0.1.

Example 6 one-step growth Curve experiment

Mixing phage and host bacteria under optimum MOI condition, culturing at 37 deg.C for 20min, centrifuging at 10000r/min for 5min, discarding supernatant, and washing precipitate with appropriate amount of LB broth. Adding 40ml LB broth, culturing at 37 deg.C with shaking at 170rpm, sampling every 10min from 0, centrifuging at 10000r/min for 5min, filtering supernatant, and measuring titer. The growth curve was plotted with time as abscissa and the log of phage titer as ordinate, as shown in fig. 3. And calculating the average cracking amount; wherein: the average lysis amount is the phage titer at the end of lysis/host bacteria concentration at the initial stage of infection.

The results are shown in FIG. 3. The titer is not obviously changed within 30min after the phage infects host bacteria, which shows that the incubation period is about 30min, the titer of the phage is obviously increased within 30-50min after infection, and then the titer is stable, which shows that the phage lysis period is about 50 min. By calculation, the average lysis amount of the phage is about 112, which indicates that the phage has extremely strong lysis and replication capacity. Therefore, the bacterial strain has obvious lysis effect within 1 hour and stronger lysis activity after the bacterial strain RDP-PA-20001 is added into the pseudomonas aeruginosa culture.

Example 7 acid-base resistance test

Adjusting pH of normal saline with dilute hydrochloric acid and dilute NaOH solution to obtain buffer solution with pH of 4, 5, 6, 7, 8, 9, 10, 11, 12, and 13, and diluting phage into 1 × 10 with the prepared buffer solution⁸pfu/mL, the diluted solution was incubated at 37 ℃ in water for 1 hour, diluted 10-fold with LB liquid medium and plated on a double-plate. Counting after inverted culture at 37 ℃ for 4-6 h. The results are shown in FIG. 4.

As shown in FIG. 4, the phage RDP-PA-20001 acted at pH 7-8 for 1 hour, and its potency was high, and it was 2.3X 10⁷fu/mL, suitable for growth in a neutral environment. At pH 12, the potency can reach 10²pfu/mL, at pH 4, still survived the phage, indicating a broad range of acid-base tolerance for this phage.

Example 8 thermal stability experiment

The titer is 10⁹The pfu/mL phage proliferated solution was dispensed into EP tubes, 1mL phage proliferated solution was added to each tube, incubated in an oven at 80 ℃ for 10min, 20min and 30min, then diluted 10-fold with LB liquid medium, and 0.1mL each of the sample and host proliferated solution was plated on a double-layer plate for counting. The results are shown in FIG. 5.

As can be seen from FIG. 5, the titer of the phage decreased to 10 with the lapse of time⁶pfu/mL, the titer was still high. The bacteriophage is shown to have excellent high temperature resistance.

Example 9 cracking Rate test and cracking Rate comparison test

(1) Cracking rate test: under aseptic conditions, 1mL of sample and 1mL of host bacterium solution (1X 10)⁵CFU/mL), incubating for 15 minutes at 37 ℃, mixing uniformly, and diluting to 10 with physiological saline^-1-10^-3Each gradient was 100. mu.L spread on LB agar plates, incubated at 37 ℃ for 24 hours, and each gradient was repeated twiceNext, the process is carried out. Simultaneously taking 1mL of physiological saline and 1mL of host bacterium liquid (1X 10)⁵CFU/mL) as a blank, repeat the above steps. Plates with 30-300 colonies were picked for enumeration. The test was repeated 3 times and the average was taken. The phage lysis rate was (1-number of treated colonies/number of control colonies) × 100%.

(2) Comparative experiment of cracking ratio

The RDP-PA-20001 is compared with the currently existing Pseudomonas aeruginosa phages YAPa, PaP3 and Pse-AE-P3 in terms of the cracking rate, and the cracking rate is respectively determined by referring to the above cracking rate experimental method. The results are shown in Table 2.

TABLE 2

Name of bacteriophage	Cracking Rate (%)
		RDP-VP-19003	98
YAPa	79
		PaP3	72
Pse-AE-P3	68

As can be seen from Table 2, the lysis rate of RDP-PA-20001 is obviously higher than that of other 3 bacteriophages, the lysis rate of the RDP-PA-20001 to host bacteria is the highest and is 98%, the growth of pseudomonas aeruginosa can be effectively inhibited, and the bacteriophage has the potential of replacing antibiotics to treat diseases caused by the pseudomonas aeruginosa and wide application prospect.

It should be noted that the specific embodiments are merely representative examples of the present invention, and it is obvious that the technical solution of the present invention is not limited to the above-mentioned examples, and many variations are possible. Those skilled in the art, having the benefit of this disclosure and the benefit of this written description, will appreciate that other embodiments can be devised which do not depart from the specific details disclosed herein.

Sequence listing

<110> Ray union (Qingdao) bioengineering Co., Ltd

<120> high-lytic pseudomonas aeruginosa bacteriophage RDP-PA-20001 and application thereof

<160> 1

<170> SIPOSequenceListing 1.0

<210> 1

<211> 92178

<212> DNA

<213> 2 Ambystoma laterale x Ambystoma jeffersonianum

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gttttgtcga tccttctagc cttcattggg atgttcctcg acatggaata ctctcgtcta 5160

aaatacggca cggagcctgt agtggagttc caagccagtc ccgatcaaat catagaaagc 5220

aagcgaacac tgtgcgagta cagaaagcct tgcagactgc tggcagaagc ggtctacttc 5280

gaagcccgtg gagagccgga aatcgggcag attgctgtag ctcataccgt catcaacagg 5340

gtgcagcacg ataggtggcc cagcaccgta gctggagtgt tatacttcaa atgtcaatat 5400

tcgtacacct gcgatggtag catgcggcag ggcgtcaagg agcccgacaa gtgggagaca 5460

gcaaaacatg ttgcatacaa cgctctgtat gggtatactg ttgatccaac gggaggagcg 5520

gaccactacc tgaacgagag aaaggtaagg agagccaata atggcaggct ccctcggtgg 5580

gctcggaagt acaagcgtac catcaagata ggcaaccaca cattctacaa aggagattaa 5640

catgaaaact cagctcggta ttgctatcga gatcgcactc tcggcccatg aaggtcagcg 5700

gtatggagag accaactatc cgtatgccta ccatctgaac caagtgcata caatctgcgt 5760

agcacgtaat gctccgaagg atatggaccc gaatcaggcc ttctcggact ttccgtacat 5820

ggacaccctt ctagcggtct gcttcctcca tgacgttctt gaggataccg acctgacaga 5880

ggaggactta gagtctatgg gcgtgatgcc gcatatcgta gaggctctgg tcattcttga 5940

caaaaaccga gctgaaagct atcggaagta tatcgagggt tgccgtaatc atccggttgc 6000

aaaagaggtt aaaatctgcg acactattgc caatcttaca aattctgtga tgagtggcaa 6060

tgcgaaacgg atcaagaagt atagcaacca actgagcatg ctcgaaaggg aagcatcggt 6120

tctggaaaac aaggcacaga agaagacaac ccgttccgac aaattcaaag gctacgtagg 6180

agaacagtat aatgtggaat gaatataaag agcagtacag ctacacccca gccaccaccg 6240

aggagattaa agaagttctt gcaagatggg agttcattaa attgcaggct gagtcggctg 6300

tagaggaagc catcaacaac tattctcgtc cttactcttt cttcgagaag ttgcggggtg 6360

tagacaagtg gtctccgaaa gagttactga agaaagacgc agtagaccac tggcttggtg 6420

ttgaagggca cctgtaccga gagggtaaga ttaatgcaga cacctactgg gtagtagact 6480

tccactatga acgccgagat atgctgtcag agttcaaggc aatggcccat atgaaaccgg 6540

atcgagagca tctgctggga ggggctgcta ttcgactgat ccgcaggatc aacaagattt 6600

atgaatttga aaaggaggca taatgtctgt atttttctct tccgatctcc acttcttcca 6660

taagaatgtg atcgaatact gcaacaggcc ttggactttc gaggagcaaa ccgaggagct 6720

tatttctcgg tggaactccc gggtaggtcg ggtagacgaa gtgtaccatc tgggagactt 6780

cgcatttgca ggaaagaaca agctacagcc tgtgctggat atcatcaagc agttgaatgg 6840

ccgtatcacc ttcctgaagg gaaatcattg cgactctcgt ctgtggaagt tgatcgctga 6900

tagcaaccta tcgcacgtcg aagatatccg agactacaag gaagtgagga tcgacggagt 6960

taagatcgtc atgtgccact atcctttcga gacgtggaat accgctcatc atggcgcttg 7020

gcacctgcat ggtcacactc acggtagcct gccttccaag ggtaagcggc ttgatgtagg 7080

tattgacaat cacccggacc atcaggtatt ctcttacgac gaagtgaggg aatatatgcg 7140

agagcaagag tttgtggttg tagaccacca taaataaaag gaggaaagaa tggtcaatgt 7200

gttgatttcg cgtgacaagg aggatgttaa acctaaagct aacactctgg tggaactgat 7260

ccatgacgac ggagttcctt ttatcgtcct catgacttct gatgttaaac aagacgggac 7320

ttttgaagga gtggctttcg aggacgcccg ctggacggat cagtttgaga tcgctcaatt 7380

ccgagtattc gatggcaaag taacgctgag caataaggag gactaacatg aatgatccgg 7440

tcaaggattt caagaaaact gtaatggttg ttgctgcaca ctacatcact cagtctgggc 7500

tgactcaacg ggaagtggca gagcggtgtg gaacccatca gagccgaatc tccaatctga 7560

tgaagcagca tgttgacaaa ttctctattg agagtatact ggagatcgtt cacaagctcg 7620

gaggttataa gatggtagcc tccatggaac ttgaagagca taacgtactt aacttcaaca 7680

tcttcccgga ggaacaagca tgaagattga taagaattcg tggcatatgc ggctccatcg 7740

tacgttcgag accccggtct attcggctat ctcaagcggt tggcaaattg taaccctctg 7800

ctcttatttc tggcagacag tattcctcgt cgtgtggctg gcgttaacgg ttgtagcgct 7860

cgctgctgtt gtaggctctg tattgtattc tctggctctc ggtccactta ccggatatgt 7920

tctaggtggg actctggctg tcatggggtt tgtatggatt ggaatcatcg ccgtctttta 7980

tggcgtgtgg gttttctacc gattctctcg ctttatgtcg agtaaatcgt ccagcaagga 8040

tgggattctg gtatcgtatc taaaggcaaa gaaggctaaa ttctgcccaa ttgtcgaatt 8100

gaaataagga gaagtaggat ggaaggtaat ctcaacattg caatgtacaa agacctgatc 8160

gctaataagc tggtgactgt taagcacttc aacggtatgt cgattataaa gtatgcccgg 8220

aaggtgttct acgagaacct gtggaatgag catccgctcc ttctggaggc acggggtcat 8280

gtatttgacc agcactccgg tgattgtatt gtacgcccct tcgagaaggt gtttaatctg 8340

ggagagaatg gtgctgggag tttcctccat cctaaattcc gtgtgcggct gattgagaaa 8400

gtgaatggat tcatgttctc tgtaactaag cacaacggct cgctaatctt cagcacgaca 8460

ggatcgctga gtagtgacta tgttgcacta ggtcggaagt atgtgtctga gaacgcagat 8520

gactacattg cagggttcac gtacaacttt gaaatctgct ccccggaaga ccctcacatt 8580

gtggaggaag aagaaggtgc ttatctgatc ggtgttcggg acatcttcac tggtcagcag 8640

cagtctgagc atattctgga tgcatacgca cttggtatgt cggctggaag ctcagttaga 8700

attctccgcc cagatcacat cgagtgtagc tgggcacatg ctaagggcct cctcagctcc 8760

tgtgagaaag agggttatat ggtgcggact ggcatgggga cggtgaagtg caagtccact 8820

cactacctcg gtaagaagtt tatcatgcgg atgggcagca agaaggttaa tgcgatgtac 8880

caagacccgg ctggattcaa gcagacgctt gacgaagagt tctatcctct ggtagacttc 8940

ttggtaaatg aagtagagga gtcctgtttc gtagaaatga ctgatgccca gcggcgtctt 9000

ctgatcgaaa cctacttcga tatgactaga atttaaagga gggaatatgc aggaaaagat 9060

catgtatatc attcgaggac tcccgggctc cgggaagtcc tcatttgccc ggattttata 9120

ccaccagatt tgggagtctg gtgggacggc atccatctgc gaggctgatg actaccagat 9180

ggagtatgga gagtacgtgt ggaaggcaga gcgtgtagta gagtcccata agaagtgcat 9240

ggagaaagcc cgaaactcta tgaaggccgg tcttgacgct gtgatcgtgt ccaatactag 9300

cacccgggag cgagaggtgg aaccgtacat tgacatggct gaagagtttg gctacactgt 9360

gatctctctg attgtagaga atcgtcatgg gaataagtct gtacacgacg tccctgagag 9420

caccatggaa gcaatgcgga agcgcttctc tgtgaaactg tgaggaggat gtatgattag 9480

ttatgctgta ggttgtcttc acgaaatgga aggtgttatg agccgtccgt ggcaagaggc 9540

aaaagtgtgg acgttcccgg gcggcgaagt gggcgtcaat gtggcaggca tcgaaagcca 9600

tgcgttcgta aacgttaaag ctcagattaa gaacagcgat gatatcatgg ctctcctcaa 9660

tgtcactgaa gcgatccgag cagaaatgat taatccacac atgacgcttc tgctaggtta 9720

tgtcccgtat gcccgccaag accgtcggtg caatccgggc gacgccttca gtttgaaagt 9780

gtttgcaaac atgctgaact ctgccgggta cgaccgagtt atcgttgtgg accctcacag 9840

cgacgcaact ctgcacctga ttaacaacgt cgaagttgtt cgtcaatctt cggtcttcgg 9900

cggcctgctg aaccgctcag agtgggagca gtaccaactc gtagcccctg atatcggagc 9960

aacaaagcgg accgaggatt ttgcaaagaa aactggtgca gccactgtaa ttcgttgcaa 10020

caagaagcgc gatcttcaga ccggcaaaat cgaaggcttt gaaattctga atccttcgga 10080

tattgatcct gagcttccgc ttctagtgat cgacgatatc tgtgacggag gtgctacatt 10140

ccgaagcgtg tctaaggccc taagagagta cagtaaagtg ctccctgacc tgttcgtcac 10200

tcatggaatc ttcagtaagg gtatcgactg cttgctggat tccttcggat gcatctacac 10260

ctcggatacg ctccctcaaa aagagcacga agcgctgact gtcattccgg ttgaatactc 10320

caaggcggaa cgcctgtgag cttgagtatc tggattgtag agtataagcg ttcggctcca 10380

gagaagaggg ttgtcaaggt ggaggagttc gacaccaagg aggaactgga cgctcggtac 10440

aagacgatgc acgataagtt ttgggaagac ttccacagca agaagcaacc attgctgcgc 10500

cttccatgca tctaccctgt gctgacgctg ccagtagaag aagtcgaata ttgacaggag 10560

gcccagattg atgtagtctg ggcctattca acacaacagg aggaaataac atgaaaattc 10620

tgccacatct ttccaccgat ggttataaga ttggacacgg ttccatgtac tcggaaggga 10680

ctcagcttgt ctatagtaac ctgacccctc gctccgataa gatttatcga aagacgcatg 10740

ccaccgactt ctacgacggc actctcaagt gggtcggcgg acgggccgct tggatggaga 10800

tcgtagagtc ttgggaaggc ttcttcgcgg ctgaagaaga gaacgccgtt ggccggtatg 10860

cccggttcct gactggctat tttggccggg aaatggaggc tgtggagcag cttcgagccc 10920

tacacaagct cggctatctg cctctggagg tcaagtctct gactgaaggc gtcagtgtac 10980

cgatgggcgt gcctgttctc accatcaaga acaccctgcc ggagttctac tggctagtga 11040

actaccacga aacactgatt tctgctatga cttggaagac cgcgactaac gccactatcg 11100

ccgcagaata cgcagctatg ggccgatact acgcgaatca cactggatgc tatgacgact 11160

tcctgctgtc cgttcagttt cacgacttca gtgcccgggg tatgagtggg attgaagacg 11220

cggcccgaag tggtgttggt cacttgacct cgttcgtagg ctcggacacc ttggcagcgg 11280

ctatctacgc cgaggactac tataacgcga ctggtttggt agcttgctcc gttcctgcga 11340

ctgaacacgc tgtagctact agcaacatcc tccgcattga gaaagaaatg gaggatggag 11400

aaggtaaagg tggccgtgag gaagcagaac gtcgattcct tgttgaactg atgggtaagt 11460

tcccgagtgg tattctgtct tacgtggcag acagcttcga cttctggggc gtgcttactg 11520

acattctgcc atccatcaag gatcagatca tggagcgaga tggcaagctg gttattcgtc 11580

cagactctgg tgatcctgta gaggttatct gcggactggg tgatattgaa gttgtcgagt 11640

cgctcgaaca ctgggatatc atggatgcag taaatggaaa cgatgcattt gttctgaact 11700

ttgaagataa gtacttcaag gttattccca ctgagtggga tcaatacgag tatgtaattg 11760

gctataaact tgaagaagtg agcgaggcgc aggtgaaagg agctatccaa gttctgtggg 11820

aaaccttcgg cggtactgtg aacgaagcag gctataagat gctcgattct catatcggtt 11880

tgatctatgg agattccatt acaactcgcc gagcagctaa aatcttggag cgcttgaagg 11940

agaaaggttt cgcttccctg aacgtagtgt ttggagttgg cagttacact taccagtgca 12000

acacccggga caccttcgga ttcgcagtga aagctaccta cacggaagtg aacggtgaag 12060

gcattgcaat cttcaaagac ccgaaaactg acagcaagaa gaagtcggct aaaggcctcc 12120

tgaaagtgta ccgagacctg caaaatgggc gattccccca gttgaaagat aatgtatctg 12180

aagaggagga ggctactggt tccttgcgga cgttcttcaa agacgggaaa tgggacaccg 12240

aagacctgaa gaacaatgac ttggcatcta tccgttatcg gattaagaac ggagtataag 12300

gaggataaag gtgttagcta aatattactt tacaaccttc tgcgaacaag gtcgcccaga 12360

aaataaaggg cacaaaatgt tcaagttcaa aatctgggag aaggagtatt ggtccccgtt 12420

tgctgccact aactcgatgt acgcagacat tgctcacgga gcgagaggct ctgaagtcag 12480

attcacaagt gttgagaggt tcaaatgatt aaataccgaa agggcgatct tatcgctgcg 12540

ttcaaaaaca aagagttgca cgccattgca catcaggcta attgcttcaa caccatgggc 12600

agcggcattg ctaagcaaat ccgggagcaa ttcccggagg cctacgaggc agaccaagca 12660

acggtgaaag gggaccgagg taaactcggc tccctgacct tcacaatgaa cgagtacgga 12720

tcgatttaca atctgtacgg gcagtacaac tatggccgag aagagggacg agtctacact 12780

cagcttgacg cactgggcca agccctgtac actatgtctc tgttccttga ggcagaggct 12840

gacgctcggg cgattcctac ctcggagttg tgtgtaggtc tgccgaagat tggctgcggt 12900

ttagccggag aggactggag caatgtcgag cctcttatcg aggagacgct cggccagttt 12960

caggtatacg tttacgaact gtaataggag gagatatgat ccggggtgca attaagtata 13020

aggggacttg gttggccccg ggctcggagg ctttccagct ttatcaggat aaaaagctag 13080

acaagttgga taagcatcta gccaagctgg acaaggcatt caagaaacta gaagggaggg 13140

agtgacatgt ctattgatct ggacaactta acagcgtacg aacttctcta tttgcaagct 13200

caaataatcg agaaattgag actcaagttt gaagagtctg gagaaggtga gctagagata 13260

aaagacgtgt accggggaat gctcttaacc atgggcagtg atggatacac tattcccaga 13320

ggatgtgaat gcaaggtggt aagcatagac cacgaagaca acttctatcc ggtgttagtg 13380

gagtacacca accaagaagg ggattatcgt caccactgga ttacatacgg agaactggga 13440

caaatggtga gacgatgaga tattatacag gagtcggaag tagggatacg ccaaacagag 13500

agtggggcat ccttcgagag gtaggccgta aactggcaga acaaggtttt attcttcggt 13560

caggaggagc gaagggagcg gactcagcgt tcgaatcggg ctggctggat tatatatcca 13620

ataacaaggg aggaactacg gcagaaatct atcttccgtc tcccggcttc aaccagtacc 13680

agataacgga gacgctcgga gctaccatcc tgccagagga agagttgccg gctgaatggg 13740

aagaagctaa aggcattgcc agtcaaattc atccggcttg gaactctgtc agaaatgacg 13800

ggaggtccgt tctaagcccg ttcgcaagac gagcccacac tcggaacgtt ttccaagtgc 13860

tgggaatcga tctcaacacg cctagcaagt tcttggtgtg ctggagcaag acagaccaca 13920

acgggattcc agaaggcggg actaggaccg cttggcaact ggcgtcctct agaggtgtgc 13980

cttgcttcaa tctgtaccgc catgcagata gagacagaat catggcgttc ttgtaaaaca 14040

aatagaggta caatcatgta tacagttgag gttccagagg ttccggtagt ctgcgatatc 14100

acagacccaa accgcaggtg catgcaatgg attcgcgctc gaatcatccg acttaagaac 14160

aaccagagta cagggttcga agacatttac gttctagagc ctagccacgt tgatctagag 14220

tgcagatggc gagaagagaa cgaccctgaa gagcacgtta gatgggctat tgcgtggttc 14280

gacgtggata tgtgcgacgg gcagaagttc cgccagttga ttcgatacga tatcaacaac 14340

atgttcaagt ggtatggacc tgctaacctg acatgcgaag gcgtgttcac tatcgagaaa 14400

tctaaggtgt gccctccgta tgttgacatg tgaagagatc gaggagctaa agtctcggat 14460

aggggcgaat gctgatcggt tgattcacaa ggtggagagg gggcttgttc ctcctctaga 14520

taccagcaga gggcgaaagc cctctttctt tttcaacgct gttcagcctg aaatgtacga 14580

agtcatggtc gagctagacg gagactaccc gttgtgggta gacgacgttc tccaaggagt 14640

tgcccggctg gactggtaca gacccgatga aaggtcgatt cccctctctg caaagaagct 14700

cttgacaatt tttgcaagcc ttagtaatat caatgctcac acgataagcc acctcctgcg 14760

ggttgaggct agacaggcta atcggtacta cagagcagcc gaattcgcac accaaaaact 14820

gattgacgga ttctgcaagg atgagctaag atgcatgaaa tatccagaga cattcgtcta 14880

ccctaggcag gatgttcccc agacagatca agaggagggt tagtatgttc aagaaattgg 14940

tagccgttgc gtttacactt gccctgcatt catgcggggt cgctcctgct tatgcagagg 15000

acacttctgg ccgcgatgca gaatactgct cggctgtagt tgcacacttg acaagcatgc 15060

caatggaggc tatgctctct gctctggatg ttcgtcccgg ggatgatcct gttatccgag 15120

tagcaaacac catgtatatc tatacatgca aggtggaggg caatctggtg ctggtcaaag 15180

gagatttcga gaagggattc tatcgagcat tcctcgtcca agaggatgaa tacaactact 15240

acatcagccg ggaggaaatg ctatgatctt cgacaagtat attatcgcgt tagatacgtt 15300

caaggatggt actgaggaat acgccctgtt tagccggggc gacaacgggt ttataacgta 15360

catcaacagc ttcgcaacca aatactcagc tattgaccac gcgttcttct tggagtacaa 15420

tcgctaccca tcagcttatg aggaggccga cctgtacaac tgggttgtga tgtgctgacc 15480

gcaggtcgaa ccaaaaattt agagccctag tggggctctc aaaggaggaa caatgagaac 15540

tgttgcagag ttgataaaga tcggactaga agactacttg gttaacaatc aagaacctta 15600

catgtgttat atcatgagtg atctcgtaga agagcagcag atcacctcca aggagtatcg 15660

atcgttcaag gagtggctac tagaagctca cggcatctcc acttgggagt cagttaacga 15720

ttacctgcgg atcaaccggt tggaggatac cgaggtaaac tggaaaaact tttacgtttg 15780

gtcttatttc gacctgattc gaaaggaggg aaaatgaact ctaatagtat tgcaccacta 15840

atcaaggaag gcatgcgggc attcttcgag aacgaagaag agccttacat gtgctacatt 15900

ctggagagat tggcactgtg cagtgactcc agcgtcaccg tctcagacgt gggacgattt 15960

aaggggtggc tagataagac ctttggaatc gggccgctta attcggtaat gcacctcttg 16020

gacgaagtag ctgattcatg cgtgttatcc atgttcgaga ataaccctcc gaacaaggac 16080

tgggaagact attgggaagg ctgggccata ggcagagcgt ggtgcaactt ctacgtgtgg 16140

gcctacttcg acttgattcg aaaaggaggg gagtaatgga gataatccca atgctattcg 16200

tcctcgcgct cgcgtgggga attgtgttca tgtggaccta catgggtatc acggagtatt 16260

tcaaagataa cttcccagct agtgcgacta aagatcacag agagggtatc gaggcttttg 16320

ccattgtact gggactgcta tggcctatta cattcacagt gtatgctagc tttctgctac 16380

tctacgcaac atacagcgtt ttgaaagtgg tgttgttacc taagtgggct ctgtctatct 16440

ggaacagccg taaactactt aagaggatcg caccatggaa caagtagggc ttctcattct 16500

agtatgcata gtcgtcggag tggtctgcct attgttctac tctgtgatag ggatgttcgt 16560

atgggaatct ctacgacccc ttcttgggtg gaggggagaa agtacagaga ggcgtaagag 16620

agaggcccgg gcggtgttgg ctggaatatt ctggcctatc gcagtaccgt ttatggctat 16680

gctattcctc gttagtctcc ctagaaaagt gtggagactg actagggtag ctatcacagg 16740

agaggaggac tgaatatgtt taagaagctg ttcgcatggt tccgagagga agaaagtaac 16800

gatgagccta aagtgttgga gtctcgggct gaggagtttc aggtagagga gtgctatgat 16860

ccagcctccc ggcctgtaaa gtatataaac gctaagcaga ttaaacgtct gaaggaactg 16920

gatgctaagg tcggagaccc ggttaaggct atttgtgcta cattcttcga ctggaagcga 16980

tggatcgtag accgcaactg gagattttcg atgatggccc accgtatcga tgagaaggtg 17040

catattacag attctaagac cggaacagtg ctacagtgga gagctaccgc agactcagac 17100

ctgagaccgg taggattcct atggagagat atccccttgg tcagggacgc cgctgacctc 17160

gtactgacca atatgccgtg gatgacttcg gaggaaaaag aatacgctgc ccttatggct 17220

aggaaggcta tcgccgcttt ggcagataag aaggactaca tcgacaagct ggtagcccgc 17280

aagcgtagcc gggataacta caaggctggt ctgattgacc gtgcgttgat gatggagaag 17340

tatgtaggag tccaagaagg cgatgagtgg gatatcattc taaaagaggc tagagagcgt 17400

ctggaacggt ctatccccac agagagcttg acaatggact gggaacgttt tacactccca 17460

taaaacacag acaggaggaa tacaattgaa agagcgtttg gttaaatttt acactatcgt 17520

gaagaccccc gaagagaaca ttgcagtaca acagcacctt aaggcttatg gtatcgagtt 17580

tgggagcgga aacggcttca tgtacgacaa ggctcaagtg ctactgtggg ttggagataa 17640

tctgctggac tacactcgta aaagcgacgg aagtccaatg acattcctgt ggtacgagga 17700

agaactcaaa gaggccattg gatactcgaa atcgacctac gacttccgaa gggtagactt 17760

ccatttcgtc actgagaaga aagtgaccgc gtacacgctt catgagtgtg acactggtcc 17820

tactacagag ctaaatggca cctacacgct tcaagcgttg aaggaaatca ttgcagaaat 17880

ggaggaagag aatgaaaact aaagagatcg atgtcagcaa tttcactgcc gaacagtttg 17940

atgcgttcct tgagtactgt cagtacgctc aattgaaggt aggagagggc gtcgttgata 18000

agatcaggga gtggatggaa caccctatct ccgtagatgg gccagatgat agtccccggt 18060

gcctatacat ccgggatcat cccgaagacc ggttcattag ctacggaact ggcctgcgaa 18120

gcaagtggca acccgatgtg tatgacttgt atcggcctac cttccagact aagtgtgtga 18180

tgactctagg gaaggcagac aagtatgtgg tcgtaggaga cgagaccatt tcactggagg 18240

agctaaagac tatcctagct aagcaagggt gtttgctgcg tttggagggt ctaatgtctg 18300

agcaatattg gtatccagcc cggatcgggt gcataaagag tctgaaagca gtggcttggg 18360

aggcttggtc cagagacttc tgggatgatg agaatttgaa caagattctc cgtcacaacg 18420

ggttcagtcc tgaccagagc aaagaacgag cgttgcagta catggaccag attaacgaag 18480

agtactgcaa ggttatgcag ggatgcttgg aagcagagga catggaatac aatcagtaga 18540

gacaaaaagc cccgggcagc aaaagctgcc cggggctctc ttttacacag ttacggtcta 18600

gctgttgtca gcttattccc aataccaatc ctatagattt gcattggcca ttctccctgt 18660

gcaagcggag cttgctattg tatgcacgac cccaatcccc tcccagaaat aatgaccact 18720

tgaagagtgc gtttactcta tggctgggaa aataatgatg tagtgtggcc ctacgtccga 18780

gtgtatcctg ttcgggagtt gtggttctat ttccgagcta cttaaccacg aacccacctc 18840

gctaccctaa cccttagtaa attcaaaata atgggtggcc gtggtctttc cttcttagcg 18900

acagctaagc tatgcaaaac taatgccaac gggctcccgc cctgccgggt gaaatctttc 18960

cacatgttgg catggaatat gcatatacgt ttgatagctt cgctatcgga ttactctggc 19020

ggagttctcg cactctcaga gggttgacca tcgacccatc aaatcattgc aaaagggttg 19080

tctcttttcc ctgtgcaata tccttgccaa gtttgtccac tctttcaagc aacgcttgaa 19140

caacgtttac actgtgaaca tcctccatct gatagagatc gatacattca tggatggctg 19200

ctatcttggc atgttgccat gccgcatggg cttgcatagg atcgttccac gatccaaggg 19260

tagactgctt gcctagtgtc ctacactgag caacgtatac aggcatcccg tacttgtcat 19320

agcgtgctag gcggacgcct agagggatat agctatccca cgtattggca cggttattgc 19380

ttgtcttgtc gcagttggag aatagagtgt gcatgtatgt aggcgtgaac tcgcatgtat 19440

gcggagcata gagcttgttt cccgggtaga ggatgttctt gtctaggcgg tagcctacgg 19500

cgtaccctga cacattcacc acccaatcat agaacgcttc aaagcctgtc ttgggcttgg 19560

cccattcctt gcatatctca tcgcttgcga tggcacgttt acgcatggcg tagtagattt 19620

gcttagcgta atggatgtga atcttggcat gattgttgct tgtcattgtg cctccttgtg 19680

tgaatgttgg ttactcgata gcggcagcta tcaacctttt agtttaacgt atttactggg 19740

ctggagtcaa tgttttcagt gtcaccactt ccggtcgcaa atataataga ggtagagcct 19800

acacttcaca gaccactttg aaagctccgc tttcgagggg ttacccttgt catgcttatg 19860

caattcgtgt gccatgcaaa acgggatagc ggagctatca cagggtatac tatagcatgc 19920

acccgcatgc aaagtgaagt cacttttgaa gcgcatgatg aactgtatgc aatttccatt 19980

gcaaaatggg aacgggtgta agctgtcgct taacatcacc gggacaatgg tagcgcggaa 20040

aggggttata cccaaaatct actcaaaaca accaacatcc tatgctaatc gcatatggcc 20100

ttaaaacctc ctacaagcct cttactgtaa ggctttcgcc ttgatggtgc agactaacat 20160

agcgaagcta tcgcttcata tacggcctta caggccgctt aaaaccgata ggttttaggt 20220

gggttattgt ggggttattt gatagggata tctgtacagc tttcgctgtc atgctgttat 20280

acatgtaacg ttaacgttac aagtggtcta tcttgtcatg ctggtgttac ctctacagag 20340

tgttacaaca ttacatgtta attacacttg aaagctccgc tttcgcagat agaacaaagc 20400

ccgcacaagg cgggctgtta gagctttgtt ggttttagtg ggcttagtag ttgtttttga 20460

ttaggcgttt cactgccttt gcgatcacca ccacgccggt atgcctagga caatgcctac 20520

tgcgagaaat ggtaacgaga taggccatat tgcacctagg acgtagataa catctttctc 20580

tttgcgggta ggccattcaa attcagagct agcctctttt agcattagta gcattccaga 20640

gactaaagcg cctatcacgc aatagaccat tgctatgacg aaaaagagga tcagatagtt 20700

aaccattgtt cacttgcctc ctatgagaga aagcccggct gtgccgggct gggaatgggg 20760

ttagctgtga tggctgcgga tgcaccgagc ttcatcgtgg agattgcgga ttcgctcggc 20820

ccaaggatgg ccagtgaact ggtatccacg atgttccggc agcagcatca ggcggagggt 20880

tgcacagcgt tcgttctgta ggcgtgccag agtggcgttc atgatctctc ctcgattagg 20940

tctcagtgaa tggttagcga cgaagggttg cgagattgga cccacaatga ccgcaccact 21000

tcttgcctaa tgcgataacc caattgtgtt tgctgttacc gccgttgatg gctccacact 21060

tcgaacaaca gatttgaacg tttttcatgg tctttcctat gagaggcccc ggtcacccgg 21120

ggcttgttac ggattgatta cttgcggtat ttcttaatca agtcgtgggc catggcgata 21180

gcatcagagt aggtttcgcc gccagcttgc atttccttga cagccttatt gcgaagcgag 21240

ttcagcttgt ttccgttgat gctgcctttt tcatccttgg cagcttcaca agcatcgcgc 21300

agagttttca gggcttggta ggttgcttgc atcgtctttt acctattcag gtgctcaggg 21360

tgattccctt tgcttgatgg ccattctaca gcaatggcct aagctgtcaa caactttatt 21420

tgagttcctg ttcagggtaa acggagcccc ttcccgtcgg agaataccac cagattcccg 21480

aatccgatgt atgtgctgga gcttccagct tcgtgagcct tcaggcaggc atcgactata 21540

gtcatgccat ccccgggttg aacttgagtc catttggttt tagcccagcc gtaccgcccg 21600

tagtaccctt ctctgacaaa ccaaaccccg tcttccctgc gggcgtagtc gtagccgtca 21660

ttgccaatgc gggagaactt cagatttgcc tttgccattt tgctagtcct tctagcttag 21720

gagacgcctt gtgcgtctcg ataggcacag tatagggaac cctctagcct cacgtcaaca 21780

ggtattttcc ggattattct agtttttcta tagccactag tcgagcccgt agagcccgct 21840

tagtcgctcg gctcagcttc catttggcgg ggtttcgcat atccttttcc cgctgcttgg 21900

cgtccttgta ggccttaata caatctttgt aaggacggcc atagccagca caggcaaaga 21960

gcgctgccag cttcacaact cgactgtcgt ggtggaccca tcgtgccgcc aaggaaagga 22020

ctggccgtct gccgtcgaaa tggcagtatt ccgcaatgat gttatcctta tacttgttat 22080

tcatttgatg atttctcctt tgtagttgaa tcggcagagg atcgactcgc agccctcctc 22140

ttcgtcgaag aaggatagac ggataccagt atctgcggga ttgcagagga gtgatacaat 22200

ctcctgagct tgaccgaaac tccgcacaat ttgagattcc acaaacacgc cggttatggt 22260

gacaaaccta actcggcaat cgtaggtttc gaatatggtt cgaagcttca tcctagctcc 22320

ctcagttaat gcaagatttg cgttttcaga ttggggcgcg ggtcgatatc aatggcattc 22380

tcctttgagg cgaaaaggat agaccaatcc gacaggtagt cttttgctgt catatctttt 22440

tgacgtatat gctgccatgc ggtcatgtaa ctatactctt cgatctcgtt cacgggagtg 22500

gtatatgccg ggctttcaac atcagtaaac acgatccgag ggttcgccag ataaagatca 22560

ggtgactcgc attgaatgtg aaacactcgg caatcttcgt ctaaggtgac gattgcaagt 22620

cgatgattgg gtgctaccgg gctcaatccc gaatcgtatt cgtcatgaac cgcgtagtcc 22680

cggatatgct ttcgaatact ctctacagct tccataagga aactacggac agtttgaacg 22740

ctgtaggcgg aatccaaacg gataatcagg gtattcatgc ttttatctcc tattgtgagc 22800

cccggcttgc cggggctgtg ataatcagtt ggttacggtt tccatcctga ccgggcgatg 22860

tattttatca tcttgtgaag tagcttcttt cgagaggctt tcagtgacgg cggcacttga 22920

gtggtaactt tcttgctgcc gaggatagag atcatgccgt cgattgcttt gcactggtaa 22980

gagtcggtat attggcgttt cattgtcagt actcctagta ggattttctt caggctgcca 23040

gttcgagcat tgccacatca tgggcgtctt tgaacaggat agcggcttca gaggcactat 23100

acccgcgctc caccatgaat gcaacccact tttcactgct acgatcatcg cgggcttgga 23160

tggtctggcc cttgcgcagg tgagcacaat ggttaacctt gatctcggcg atgatgtact 23220

cttggatttt gatctggctc atcggggcgg tcctgatgtt gtttgctggt atgggtgcat 23280

tatggggccc ttttcgggcc ccgtcaagca ttagataccc aattccgcgt atttttccat 23340

cttacgttct gcgggagcca taatcatggc catggtggca ttttcggtcc agtcggcgat 23400

cacttcgcca gcgctgttac cgtagattgc ccacatagag ccgacgatgt tgtccgggcc 23460

gtcacccttg cggaagcgga tgatatcctc atcggtcgat tgcaggctgt cagagatttc 23520

cttgatatcg gtagacttct tcactaccca ctcgccatcg ctgccatcct tcacgctcac 23580

ggtgaagcca gtacggattg cacgcattac tgctgccatc actacagcct tttcagcccg 23640

catgcgcttt tggaccagct cacgtgcctt gatatcctcg acgagtgctg cacaatcgta 23700

ctggcggaca cccggcttgc ggtaggcgtt acgcagagcg atgcggaaca gggtggcgta 23760

gggcaggcta gtgccagagg ccttgtccat gcgggcgacg tagtgggcgt atgcgaaagt 23820

gttcattggc ttgttcatgg ggagtctccg gtttgtggtt tgtcttgctt gagggccatt 23880

ctacgcgaat ggcccaagct gtcaacccta caaaccgaag attttcaaaa tttctttcga 23940

agcctcaccg gccgagtcat cccctaggtc tagggacaat tgctcggtag gacgtggagc 24000

tacagtttgt gtgaccgagg cggacaatag tccggcgcgt accatgctag cccattggtc 24060

tatgctcacc tcttggacgc aatcagcttc cgccttgcgg aatacacatt gccggatgcc 24120

gactcttaca tgttgcttgt ctacctcata gactctgatc cgtgcggtag aatagaagga 24180

cagcacatcg tattcatcgc caaaccaatc gtctagctgc ctaggggacg tgaaggcgaa 24240

atactcaaaa tggcgaacat ctggtatccc gtcatgctca gggacaggcc gctctggagt 24300

ggaagacgaa ttcgtcatgc acccgttaac ataagggccc tcgccgttct tgcactcgat 24360

tcgataaact acagttttgt cacccatgac cgtccctcta gatcacatta tttgctggct 24420

gttctacgct tgtaatgcat tctactgccc ttttcgggct aagtcaaaga ttacgaagaa 24480

ccaaaattgc acccacattg cgtaggatga gcgcccgtat tcggacattt cctgattgtt 24540

cattttgtat gcggctgcta ggccgctttc ctcgaaatag tcatcaacgc cggcgaattc 24600

gcatccctct ggggttgatg agataccggc agtcatcatg accgagttaa ctgccatttc 24660

aacgggcgag gggaacaaat gaatctcccg gagacggatg cacatatacc acggcattcc 24720

gtactcaatt gcatagttca ggcctttttg gaaaatttca gaaagtttca tagatacgtc 24780

tcctattcgg ggattttgtt gtgaacagtg aaccgattca tatctgcctt gtgccgggtc 24840

tcgataacag gcgtaaaagg cgggaaaaca tcattcgcct tgccgtattt cactatctgc 24900

tcagcatact tctttgccag cgcatgggta gtgaacagag tcggctcaaa ctctggtcca 24960

atgctaccag tgacgatgta gcagtgattc gggctttcat tacctgcggt tcgatcaacg 25020

atggccattt tctgtatcct tttgaatggg gcgcttatgc accccttggc taggcattct 25080

acgctagttg gctagactgt caagcgttat ctttgaaatg ttcgagaatt gacagcggga 25140

gctgtgaatg gtcgatctcg aaagcttcgt atgtgtctat gtcactggac tgcgccaccg 25200

actccgctat cgccaccagt ttgtcaggta gttggtggga ttggaacata gaattacctg 25260

agactccagc tacgatgaaa gggatggccc atacactcac ctccgcctca tacacttcta 25320

caatccatga tggatcaaag ttatcctctg gaaaacagct agcttcatta ctggcgttag 25380

ggcacgaata atcatcttct acgcggtcct gaaactcttc ggggatatcg acaaccagag 25440

cgtagatttt accgccttcg gccatcatag cttgctgtaa ttgagcctgt tctaggcaac 25500

gaacgatgca ttccgcttta gcatactctt cttcatcgtg ccattccata accttgttca 25560

cgttgtagaa gtatgacatt ccgtcatgat ccgaaacgtc ccaaggtgcg gccaatcctt 25620

ccttgccttg acggttcatg atagctttca ggccttttgc agttgtccca tgaactaaca 25680

gcattttatc gatctccggg ttccagattg agcctttcgg ctgttttcct ttcttgaggc 25740

ctattctaca ggtacaaaaa cgcccgtcaa gcattatttc agcctaacgg gcgatatttt 25800

acagcattgc cagcttggga agagcatcga cagtggtttc ccagcaagct acaggacgcc 25860

acgtggtttc gtcatattcg agcatgatgg ctcggtcggt ttcctgatga atccactcct 25920

ctagcaggcc gtcattgtag gtattccagt tgatccaacc gagagccttc aaccgatgtt 25980

ctaccggaaa agctgcaaac tcagctttca gcttggacat ttgctcttct gcgtgccgca 26040

gataggcgaa acgctcggtt atgttttcac tgcactcacc gacgaagaac gtaactgtgc 26100

gtagccagcg gctctcatag tatttcactc ggcgaatgat gatggcggcg cggcgctgta 26160

gttgtttatt ggtgatatgg ggagcgatca tggcagttca tcctctgcga tcagttgaca 26220

ggtgaaatcg taatcgaaaa caaagcgcgg gtcaacctga atattgaatc ggctaaggta 26280

tcgggtcagg cgggtatcgg cattagtcgc cacccaagca tcgccgaact catcggcctc 26340

ttccactagc cgagcattac actctgcggc ttgtcccggg agcgagtgat cgataatgta 26400

atcatcacat tgaacggcag agcaaagggt cagagttaag gctaagatcg acattggagc 26460

attcctgttt tgatggagat tgcagattac agtagttcgt accgatagtc aagcttcatt 26520

tggcggcaca gtttgttcca tttcaccagc aggcggagtg cagcaggcat cggcagagcc 26580

tcgatcaaat cgggatcacc accgcagagg cccatagatt cgctgaagtg ggcatcggtg 26640

aatagtgtga tctcaccgtc ccgctttccg ccgacaaccc ggacgccttt gctccagtta 26700

gcttgagtca ttgcccttgt tcctctagtt gttcgattac agtttcccac cacaagcgcc 26760

gcatttgata ggccctatcc gatacaaaga aagtcatttc atcctctggc tcatcatcgt 26820

atacggctct ttcgtaaccc ggtatctctt tcttgagctt agcgtgcaag gtaacgaagg 26880

gccctagtag ctgatggata aattcttgta cctcggtagc tttatttctg gacacattat 26940

cgaacgccag agcgcacatg aacgaattat tcttgtaaaa cttcaggtta tttttcagaa 27000

tagcggagat tttcattctg cggccctttt ttggtcagat agtagactta gcggcatcgc 27060

ttgcggattc tttatcctcg aacggacccc aatggaaggc aataggctcg ctaaggctag 27120

ttccgaaaac gctcacgatg tagcagccat aaacatcctc atagacagta gcagtacggc 27180

tatcaccgct tgcgtagtct tcacttttga actgagctac agctttcatt ttgagtctcc 27240

taatgggcgg tttgtcttgc ttgatggcca ttctacgcga atggcctagg ctgtcaagca 27300

ttattttctg tcgtctttca gtgtgtaggg ccagcccagc gcttgccaag cccggaattt 27360

ggctaccgtt ttaagagcgt gcgtttgatt gatacagaat cctagcacgg tcacccggtc 27420

acctctgtaa ccgggctcag tacggatgat ggcccacctc tggccccgtt tctctgtcga 27480

gtaagaggcc gtcactggta acgctcgata tcacgcagag tcttaagagc atcgttgtgc 27540

cctatgctca ggttaggatg atacccgtac tcactcagag cgttgattgc agcccggtta 27600

atgcccttcc attccaccca tgctgcaatg acattcccag tcacccggac actctcagca 27660

ccatatgcaa cgaatcggga gttatggtag ctaacccgca gaatgttatg cccggctttg 27720

tttgcaatgt agcaggtaga gccgtggcca ttcattagac caccacggcc agtagaagct 27780

agctctaagg ctaggtcgat cttaggcccc aaggccttaa gctgggattt gttaagctgc 27840

cgcatcactc gcctcctttg tagcaggccg cgcccgccgc tgtctgatac tgagagggga 27900

accacttcac tccccatgcg gttagctgag cttgtagctg ccttgtcaga ttctgccctt 27960

ggctagcctt gcgagtttcc ttgtaatgaa tttccccgtc cgcttgcacc atgaccccgg 28020

acattccctg ccctagggca tcgtatatga cgattctatg cttgttcttg tgatcctgat 28080

acaagactac cttgcatccg tttggagccg ctaggctccg cacggttcgt tctaggccca 28140

tcatcttaca ctgattgaat ggtgcgtaaa cagattcgtc cattgtgtta acccttagca 28200

atgagatcga cagttttctt tgctgtgccg tgggcacgga agccgataat atactcccgg 28260

tcggccactt ggcacaagcc gcacgactta caattcacct tgtcgctttt ctcagccgga 28320

caagctacca ctttacgtcc tttcggcgta aagcttacat tctccgcgtc catcgggaga 28380

atagtcacta caggtgcgat gttcaggtcg gccagttcat cggcgtgcgc tacattgttg 28440

cccgacaggt tgatagtgaa gccgctacga ttggccgaca atacgtgctg gcggttcatg 28500

aaattgttca tcgggtaatg tgtgtaggtg aagccgcgac gtcctttgtt ggctttcacc 28560

aaatccatta gcgcaacgcc gtcgataacg tcatcctgac ctaccaaatc accagcttga 28620

ttatgacgcc acaagtcgcc acgggagata gatcgcacct gttgaaggaa ctctttccac 28680

tctacgccac gttcaccctt gcttagcttt ttccagtgaa tagcagtagg cccataagcg 28740

gcatagcagc ctttagcctt gagagggcag gcatccgggc aggtgccgtt atgagacgtg 28800

ctaacaggca tgggaccggt tttgctgttt ttggaaacga ggttaagaac gaagtgcatg 28860

acggttatcc tttatctagc taggcggcct gtccgccttt cttgctgacc attctacagg 28920

aggcgggaac cttgtcaact gtttttcaaa acaaaccaca caaaaatcga gaaagacagg 28980

atggtccaga acatccaagg ttttacactg cttaacagga tggctctacg gtgaacctct 29040

tccggtacgc tgtacccttg gacattggta ggctcatcct ctggcagacc gtaggcccgg 29100

gcacgttgct tcattatctt ccggcgtcgg gcttttagta ccttagccat ttcgttgtat 29160

tcgtccgggt ccgttgcatc cctgacccgt cgcaattgtc cctctagctt atcgacttcg 29220

ccaagcttgc taaggtaggg gtctatcctg tccctcagcg gcgtttcatc ctcggtctgg 29280

tcttgaaccc acgacgggag ccgctgtcgt ctagccataa cataccgccc attgtcccga 29340

agtcttaaag agttttatgt ccttttgagc gggcagaccg gcgaactccg gcatggcgaa 29400

caaccatgca aggcaaaaag catggcgagc atcgggacag ggtacgcatt gacgaaccat 29460

ttttggtatc tccgctgggg ttgagaatcc taaaaccact cgacgaatgg gcttaggatt 29520

gccggcgaat cggcccaccg gctaggcaca aaccttacat atcgtcgcgg aagtcgaaag 29580

ctcggcctcg gctagctttg cgattgtcac gcgatttgcg ggactcttta cgttcggaac 29640

gtttggcggc ctcgaactgg taagcggaat cgaagtcgga agccttgata gaacgtgcca 29700

ttttaaatct cctttctgtt taagtttgtc ttgcttgagg gccattctac aggaatggcc 29760

taggctgtca agcttttaat cgaatttata ttcgattccg tttaccttgg cgcgggtcag 29820

agtctcaagg ttgatagtgc ggaaagcgga tttaccctct tccatgttca gcacctcggc 29880

ggcggtatac aggtgcggct tgccagcgct ggtgggcttg cgggtagcaa actggccgtt 29940

agcatacgcc gacttgatct gacgtttaca cgtcattttc cgaacggtgc cgtccgcctt 30000

gataaactcc acactgaaga aactgccatc cttcgcttcg tcgagaatgg accgcttgcg 30060

gtctacgcta acagacttag ccattactta gcctcctttt tgttcagctt gacagcccag 30120

cgcttttcag cttgagcgcc gttgtctacc actttacggc cattggtggc cattgcacgg 30180

gcttgggtac gggttttgaa aaacatttgc atctgctttc tcctcgccta tcggcgtctt 30240

cgttgtctgt gggtgctatt ttgcagctag tgggtcaggt tgtcaagcgt ttatttcact 30300

aatgagcaca tagaattcga tcccgcgcca agcccctccc accttggcca tatcaccttg 30360

tatcttactc actcggccag tccatatttc tcccgtgccg gcatgcttga ggaacttacc 30420

acctaccaat tgttcgggcg tgtattggca cgttccgtcg agctttttcc gaatgtcacc 30480

ccggatagcc acgatatctc ctactctgac ggtcatcagt tctcccctca tgccgttttt 30540

acttcgatag accgagcgac aatcggtccg tcctgcccga tattgtacga taccaccggg 30600

agcgattccc atcgacggag catccattct gttttatcgc agtcgtctgc gaaaggcggc 30660

aggccgtcgc ggaaagcgat ctcatccgca ataagattaa gtatatcgtc caacccctcg 30720

tctaggtcga gcgttcgacc aagcaaggcg aaggcttgaa tcaaggcctt gtaactcagt 30780

tccgacagaa cgattttaac agaaaggtta tccatcactc ggcctcctct acagtgatat 30840

cggatgggta tccatcatga tttaccagag acttccgcac ctcctcggta tcacacataa 30900

catcgaagaa aacaatatcg atcaactgtc cgttctggta tacgttccaa gcttgcattt 30960

ctgaatctcc tttatgctgt gcggaaagag cgtgtgtccg acttcacgtc aaagataacc 31020

ccgacgcctt ggacggttcc attgcagtaa aggctgtgcc aacgggctgt acccttcgaa 31080

tgcttcacca gataggtgcg gcgacgatac atgtatgcgc cgtcttccca ttcatcatct 31140

ttctcgatag ggaagtagcg gttcaaatct accgcaatac gatcaagctc gctacgattg 31200

taagccgcgt agagtgcccg atgattacaa agagctttca tatctgcttc ctgcgtgggt 31260

gctcagggtg tttccctttg cttgagggcc attctacgcg aatggcccaa gctgtcaaca 31320

tctttttctc aattattttc gttcttcacc caaactagcc tgcccagcgt ttcaggacgc 31380

ccacgtacct gttttctcgc catgctagtc cgtctgaaac cgtaatggtt gagtagttca 31440

agctggccgc ctccgtgatg gatggccagt cgccacggat agacgactac cttttcctta 31500

gtgctggcgt attggttcgg tttggccccg atcaattcgc accactcttt ccataccgcg 31560

ccgtggccct gccctcgtcc agctttggcg tgggctatct cgtggcgaat ggtatccaaa 31620

acagcaggca agccgttgtt agctacataa tcagagccta gctctatgag ctttttagta 31680

taatggcatc gacctaatgt agccttcagc ttactattga atctgaattt ccagccttgt 31740

ttgaggagac cccatttgtc catttcctgt agggccaatt cccgggcgtc attgtgcaac 31800

attgtagagt accgttccgt tgaatgttgg ctcattgtgc ctatatagag acagcctgtc 31860

aacctatttt atgtagattt ttctctatat tttcgcttgc cttcttttct tgcttgtgta 31920

taatgacagg caggcgggca acgcaggcag gaggccgagc accggaggca aacacggagg 31980

gagcctgaga tttaggttgg cacgctaatt gcaattgcaa agactgtgcc ataaaaatta 32040

tgttgacaaa ggggtttgtc atagtagaat gggcacatct taaacgcggg gtgcgagtcc 32100

ccaactacat atcgccttca cgacggatag tcgaggccta cgcctgcccg ggaaactggt 32160

gcgggcttgc aacgttcgtg ccaactgagg tccgtcctag gaatttttag ttagtgtgct 32220

aacgattaaa ggtagcttgc taaccaatta gatagcctgc taacgatcca cccttctgag 32280

aaatccggct aaatttcagg ggaaattcct ctgtagaaaa atccccttct gtgaaaaact 32340

gtccagttcc taaaaatctg cccagttctc aattttgcag tctgtgaaaa tctatccagt 32400

ccctaaaaat ccatccggtc cctagcttag atcgcctcgt attcctcccg tttaattctg 32460

tgcagtactc ttcgctgata ccagctaatt aagtatgcta tacaagccct cttctcgatc 32520

tctgtggcat ccatctgaga aacaaactct ttgatatccg ccagccaata cgcatattcg 32580

ccaccgttgt caatcttcat tctaaccctc ctattaagcg tcacactgtc cttctacggc 32640

taagtagtag ctcaccctgc ccacactcga acggctctta tacgagcatt ctgagccttt 32700

tcctgagaaa tttcatattg cccatattgg ccgaaactcc cagaaatcta aaaataattt 32760

gaaaataacc cctgccacga aaaagcccgg ctctcgccgg gcaattctaa aataatggtg 32820

gcttattttc gccactctgc tccgaccacg gtccattcgc gggtccaccg cacctccgaa 32880

gagctgtgca gcttatccca caggggatca tccacactaa tacacttaac taccctatcc 32940

ggtacagagt aataggaaga tgggcggtcg tctttctcgt tgtactccca ttttacacca 33000

ttcactaggt cgaggttttg aagttcatcc ggtacatttc cagattgaat gtcggttgcc 33060

tttggagaca ggataaaatg gatactccac cgctttccta ccggcttagc gaacacacta 33120

acccacttcg aatctttctc agtgaattct tttctatcta gttccctctc tagatggtgg 33180

taaccccacc gacccccacc ccaccctcgg ctagagtacc ggcccttttt agctaagagg 33240

tgccaagtga tcttcacctg tccgaggtag acagcctcta gtttctcctc tgtcccgtag 33300

ctaagaagag tgcatcgatc ccctgcaacc atatctttga cggttttggc cgcctctttt 33360

cgctctacga aacttacagc ttctttgtag agagaggagc cttccggcag tagaacgtgc 33420

tgagagccca tccgaaccca cacgcaaggc tcttgaataa ctcccttgat tactgtgcag 33480

ttgtagagca aggtcgagat attcccggta ggaacctcta gaacaaagcc cctagggtcc 33540

gtcacacgga aatatttgtt ctcagtgacc cagcgttcaa cactgtcccc gatgaaatgc 33600

cccgagacag ggatgttgtc tatgaagcaa gtctcggcat tcttacctgc ccagctcata 33660

ccagtctgct gctttttctt gaagctggca gccaagctac catctttatt gaagtccact 33720

tgagacatgt atgccaaaag cgtcccttta tagtcgccct tccgctcgct tttagctacc 33780

catagttgct tcgatatatt gaattgcatt tttcttccct cctctgttgg atgccgtaag 33840

tttacatatt ccggccaacc ttgtcaaccg caaatttcag gcaaaaagaa gcccgccttg 33900

tgggcgggct gaaagtgtgg cgtccttgcc actaaccatg atttgaatct ctacctacaa 33960

cacacctaat tacctaatta ccaaaggtcg tctgggtagg cttggccgga ctctcgaata 34020

gccaagtggc gttctagaaa ttcgatatga gcatcaaacc atgcacggat gcgttggttt 34080

ggtgcaaagc gtctagcttc tttgaactct tcgagttcct gccgcagacg acgcaccgag 34140

tacttacgat acacggcctc tttccagcgg ctagtcattg ctagtttcct tatattgagt 34200

agggaatttc ctacatacca ttgtaccatt cgtcggctaa ttagtcaatc tcaggctgcg 34260

gaccgggcca tacagcctcc gggtctcgaa tccaagcctt gatctcggcg agtgtcttaa 34320

acccctctga tttaatcttg ccttcgcatc cgtaagggta gtaatgccac ttcttaccct 34380

gtttcccaag ccagcctagc ctcttcttgc cgaatcggac gattactcga ccgtccggag 34440

tgacaggtag gaagtttacg cacttgacgg ttcttgccat ttcaccactc catgctgaca 34500

gtgacggtct tagtcttgtc gtacatgttg cggagaatcg cttcccagcc cactgccgtg 34560

ggatatacag catttacctc gtaggtttta ccgtcagatc gcagtcgaac aacatcacct 34620

gccttggggc gcttgtcgag ggacagggtt tctacttctt taggctgtgc tttcatgagt 34680

cctcctcaca tcttgctgaa gttttgtttg tagtcattgt acacgctgtc gaacagattc 34740

gcaatagcct gaatatgtac ttcagacggg gtgccagccg ggagatactt acctttcggg 34800

gtaatcagag aggttgcttc ctgcccgttc tcaccagtat acgtcacagc cgcgtttact 34860

gcaatcttaa tatcgcgggc cgtagcctcg cccgggcgca taacctgttt cacttcgcta 34920

agaacacggt ccttggtggt gaattcacaa gcaggtttgc ggtcttctac cttgatgcac 34980

atgctataca gcagcttctc gctcttcacg gagcgaccga gattgatggt gccgtcttca 35040

gtagacacga caatatcctc ggcgggcgcg ctcttgttgg ccaacgcagt aatcccgact 35100

acaaaaccta ttgctgcgag agtcagagcg acaacgacaa tcaaagcctg cacaatctct 35160

ttcatattca tcagagcacc tcctagttaa ttttggaatc cagtacgttc aacacagcct 35220

cagcagcttc acgaagcttt acacggagct tgtcgcagtc ctcgatactc acctgctcat 35280

tcatttcagc catagtgata agcccgtgaa tataccctgt gcgattagat aacgccctgt 35340

cacggaagtt ctccgagtac ccagttacgt cctcttgcaa gcgttcgata caggccgaga 35400

catactcgat gatcttctct gttgtagcca tggctcctcc tctgtctgat gtggccatct 35460

tactagcact tctattcgtt gtcaaccctc ttgctgtctt cgatatcctg tagaagctgc 35520

atcctgacga acggtttatc tttcgttgcc ttctcgcaat cagggcagta gatagtgcat 35580

actcctcgga cgtacagttt acctccgcag gcgtagcaca tgttaatgcc tagcgagtct 35640

acaacattag gcttcatatg cttcactccg gctcagtaca caagctagta ggcaggggct 35700

cttatcgaag ctgccttgga aatgcccata gtttgcagat tccccgcaac aagggcaaac 35760

gtagcccggt agagtataga tagcttcgtc ttgatacacc cattggatat cttccagtag 35820

ggttttcacg acacgcatat cacacttagc gatagccacc ttagccttga gttcggctac 35880

ctctttttct aactcaccga ctcgccttga gaaagcgtcc cggtcataca taacttgcat 35940

gtatttatcg aacatatcca ttagtctacc tcttctagga tgatgtagcc cggatacagg 36000

aatacttcag cgataatccc ttgacgaact ccgagttcaa cgcctgtgac accatttaca 36060

tggatttgac catgcttgtc ctttgtccac tgaacgcctt taagcaggtt ataggcggcc 36120

ttctcgcaca aggtcagaac gtgccgacct tcgttatcaa gcacaatata ccgagattta 36180

tcaatagttt gttccattat actacctcct caaaagttgg aactaagatt cgttgctgtc 36240

cattgaatac cggtttatca ggctctacgt cattgtactc ccagatgctg atgacgcgat 36300

tatccgcagt tacgcaagtg aattcgcagt acttagcccc tccccacgca atcgctacgt 36360

ccatagcttg cgtcgctgaa tcgaacggtt cactggtctg cattagtcgc tttttaaaat 36420

attctttcat taaaacacct ccagtcctgt ttcgcggata gcttctttgc agcgattgat 36480

tgtgtacttt tggatgtcga taaatccgcc ttctggcgcg ggaagctcaa cactcacttt 36540

tcttcgacta gccatccaga tatattccgc ccatcctttg gcacaggatt tacgaagttc 36600

ttcttgctcg tcctgtcccc accatttatc gaactctttg cggagtttgc tgttcatcgc 36660

gtgaactcca caacccatac agggttgatc ccgccagtga agtctcgacg gattgtagca 36720

gtccatccca tatctatcaa gatatttgta cgagagcacg ctgcgctcca agtgtagtac 36780

acttcagaga atttgttcat tttagtccct ccttgttttg atgggctcag aatacacaaa 36840

ggcccggaag ctgtcaagcc ccgggccaat gattttacat gcacatttca cattcttctg 36900

ttctagagat atccccagca cctcgcatac tatagatgta gtatagcgac aggatgttag 36960

ggtcttccat ggccagacga tggatgtatg aaatgtaagc cggcgagtcg ttcgacgtga 37020

agtagaggtt gatcgactgc ccttggtcga tgtacttctg ccgctgagag cacagacgca 37080

ggtagtcttc cattctcacc tcgaaggcag tacggaacac agccttttcg tgatctgtca 37140

gccagtctac gtgctgaaca ctccctttac gttcgttaat ctgccttgca cactcttccc 37200

aatcgaggcc tcgctcttgg ataatctcca gcaacacctt gttgacacgg aagaattcac 37260

ctccagcgct ctgcttggtg aagcacatag caacgtccag tccgatgccc tcagaagccc 37320

cagccatgag ttctgccgtc gatttagttg gcggcatcat cattgtagtg gcattacgct 37380

tccctagtcc acggcagccc tctggctccc ctagaacctc agctagccaa ctgttacaag 37440

cttccgcttg ctccttcata cccttgaaga tggcgttgtt caggaacata gcatccatac 37500

taccgaccgg gaatcgctcc cgctgtagca gggtgtggaa ccctagaaca ccgctgccaa 37560

gcgccctgaa gtccttggtg aatcggtaaa tcttctccag tgctcggcgg tcctgaacgc 37620

tgacctcatc catggttgct aggtattcgc tgatgttgca atcagacata acctgaccaa 37680

taaacacaag gtgcttaggc caagaacggt aaagttcgag gttgtagttc aggataacgc 37740

agctaaaagt ataccagtca tttgccggga gaacagtctc ttgacaaagg ttggacgctt 37800

cacagcgtag acccgcccgt ttaaacggct ctgcaaggtg ccgattcatc ttatcgataa 37860

acgtgaagta gccttttcct ctggccagct tgacgaacat cattcgcttc catcgcttca 37920

aggtctccgg gtctttctcg gccatatcct tgcaccactg gtcgtctaga agccatccga 37980

cgttgttcga ctctgtgcgc ttatacaggt gatcgataac gttccagaag tctccatggc 38040

gggggttgat gctgtatgca cagctaccac gcctagaacc ctgtgctacc tcttccatga 38100

cgttgataac gtcccgaatg acaggcatca caccttgaga ctcaccgcct cggatagcgt 38160

ctccttctga tggccagttg gtcaggctaa agcttgtgcc atggctgtgc ttcgttagca 38220

ctgcgatctc tgtcattaca ttgtaacggt catagaggtt gttgtccatc agaccgccag 38280

cacaagatac cgtagtgccc ttcttgcgga gcccgccgtt cgacaggagc ggagtggatg 38340

ggctgacaaa tccatcccac ataacgtcga agaatacctg ttcccagtcc ttgcctctag 38400

tgtattcgtc ctcctcccac cactcaggat aaaccgtggg ggcgtgctga gccatagctc 38460

tggctacagt ctcaaaacga gagcgaacag attcgccctc attcgaatat ttcttcatga 38520

acaactggta gccagccgta gtataccaca gaggagtctc tcctgctgct tgactgtttt 38580

tcctagcttc ggaatagttg aatgtgtctc tcttactcat atttcagctc ctcttcccaa 38640

gcctttgcta gttcctcttc atcccacccc agttcgtatt ccatgcccat tcccggagta 38700

aagaagtcaa tcattttgta agcgtacgtg ttcatttcga accaatcttt gatcggagtg 38760

tcatcgttct cgaaatgatc ttccagaccc agacgacggc accatacgtt caaacgataa 38820

cgaacaaacg ctttatagtt ttcagccgac tcgccgttaa gttctccgcc cgggatcgcc 38880

atatcgacaa tacgacattc atgagcatat acatggtcga tagcttgtcg aatcttctcg 38940

acacgccgag tatcttcaga caatggtcga ccaatctctg cgtagtattg gttgatgatc 39000

tcagccgaaa ctacaccgtg caagtcttca tccacagcac tctggttagc cccgcgtgcg 39060

atgacaggga tatcgttata cccgttgcac tggaagctct tcaggatagc gaaagaactg 39120

aacagaaggg cagtctctgt taggctgaag ataattaccg aaagaacatc gtcttcatcg 39180

cctagaacag actcaagcca gtccatccga cccataagct cggggtcgtc cttgtaagct 39240

acatagtccg catctgtatc catccccagc actacgttga tctggttgta gaattcagcg 39300

tggactgcca attccatcat gcacaggatc gaagccgcta gtttaacctc tgggcgaggg 39360

aacgttcgga ctactttacc catccaaaac tcctcaccta cgatcagttc gtatttgagg 39420

aacagttgaa ggacagtctt tacagcgtga agctgatgag gttccaactt aaacttcagg 39480

ttcaacttat ccagctctac cttcatttcg ctagagaacc acaacttctc ttctagttgc 39540

tggtttgcta gttcgacaat gaccggatat ttccgggcat aggactccgt tggagtcctg 39600

atttgtgtga tcttattgaa aatatcggcc tcatctacga gagcctttgt aacttccata 39660

tctactccta ctacattaat tgtggaacgt tgcctctgtc gatccctctg gtcgcatctt 39720

acgaagagtg aaccttagat tactatcatc acacgctgca atatgagctt ccagagaggc 39780

gcaaccgagg ctcagccact ccttgtctgt acgctcaaag ccagagaatc gtagaccctc 39840

tacgactcgg ccaatcaggg tgcgatgctg acagaaaacc atttcatacg agtagttttt 39900

aacatccatg ccgttagtcc acagatgctc ccgcactact ttttcattat tactctggaa 39960

cgcctctttg aagccatcga ccagcatgag gtcttccaga ctcaggttat attctgccat 40020

ttttaaactc cttcctcttc ttggccgtag tactcattac atacgtgccc tttaattagt 40080

tgacgatgtt ggatgaaacc tcggaagtta ccagaccaga ggtcaccata ttggtccaag 40140

tgcgtcacac cgtcctccca tccagtttga ttgtccgaga tgcggagcat cggtgttgct 40200

tggtgttcga acgggctagc gtgaacgggc ttagtatcta ccaatcggtt gtagattaca 40260

agagctttct ccagagtctc gtcgttcttc ctatagctaa cctgagcaca gcagctagca 40320

gataccttaa tagcatcctc tacgctcagt tcttgcccgt tacaatctgc gtaacgcagc 40380

ttaccatccc ctatgcggaa ccgagtcaca taaggaacat gccactctcc gtcatataga 40440

gcctctgccg ggcccgcttt aataagagcc tcatacattt tctcagcgag aaccttgatc 40500

tccggttgtg cgtctttatg gcaacgcagg tagaagaagt tatctagttc cgtggctgta 40560

acaacggtct tcatcatctg gaacggctca aggagacggt taaccacctg cttgtggagt 40620

cccattttat ttagtgcgct agcgatccga gccgcactct ttgcagcctt cttccacagg 40680

tactgagagc tacgttgcaa acactcactc agttcgaact cagcttgcat acccggttga 40740

ttcattcccc agtgaacagg catcgcagga ttctcttcca cctgtcggat catatgggca 40800

acgggaatgg cacgactact tgcagcattt cgactaaaaa gtcggtgagt cataagttca 40860

ctgtgaataa accgaggata ctccagttcg aatgtagtga tgaatctgtg attcttcgta 40920

cctaggccga tccgggaact gtgttgaatc acttttgcag aaatttgcat aaactctcct 40980

tattaaatct ttgatacaat aaagggagcc gaagctccct tgttttactg aagcattgcg 41040

aaggtgaggc accagacgat ctcgctagcg aggataggat gcaccatcat gagcttctgt 41100

tttactttct ggatcatatc tacacacctc cttcgtttca acttggagcc tattatcgca 41160

agcctcgggg agaatgtcaa cccctttctc tgagaatctt atcgatctcc aactgagcga 41220

agaagatgac tttctcgtag tcccggataa catcgttacc ggccttcagc ttgcctaggt 41280

tctgtgctgc cgcagcccgc cacaacgctt tgaacatgtt gccttgaccg aatcgcatat 41340

ccagcgcttc gatgatatcg ttgcactctg caaaatacgg catgttagcc ttcgtagtgg 41400

ggttgttcac cttgacagtg tagtaactag aggagcctcc gctacctaca gcctccttgt 41460

ggttagccat agcttccaat gtggcatagt tttctttgcc taggtaatct tctaggttgg 41520

cgaacacaac cttcttaccc agtgcaacaa gctcttcatt gagtctgtcg acctcggcta 41580

aagcctcgtc cctctcactt agttcgtcaa cttctttgtg ctctactgtc tcgggattcc 41640

cagacatagc tgaaagttcc tcatcgtcgg cagtgtaagg cgacagaaca tttttaagaa 41700

cagccatgtt gaatagtggc atccctttag ggtcgccgcc aatcagaaag tctccgtagt 41760

agcgctcact gtttgtcaaa ggctgagccc gatcccagtt aacaaaggta gtcactgggt 41820

gatcttcgaa cagaacatca tacgcttgtt taacgtcgcc gagactggct gcgtcataac 41880

ccctctccat taggaggaag aaatcagaga atctacatac cttaacagag tgctcatcat 41940

ctgcactact agccagcctt acgaccaatt ctccctttcg tatatcaccc cttttctcgg 42000

taacaaccaa gactgttccg taagggatgg tatcggcgtc ccaatattga atattgatag 42060

gattctttga ccgttgcacc agagaaatcg gttgcccagc atgaacgttg atgatatcgt 42120

tactcaaagt tcacctccat atctttcaac tcttgaaacc cgtcaagact ctcgatctta 42180

gcgcacatca ctctcatagt gacatatccc ttcagtacac ccttcggacc tttaaactga 42240

ctctctagga aggccagcag ggcatctagc cctgctgagt caaccatgag atcgtcgaca 42300

gataggtctt cttttacaat ctcttcggcc acttgttact cctcgtcgct aatcgattca 42360

cgaatgcagt ccagaatgaa ggacaggttc atgtgcagag tcaggagatc gtctttggtc 42420

tccggcatcg cagcaaccaa gatcatcagc ttcaggaagt cagggccacc cattaccaga 42480

ggcagatcac ccaagctggt gcggatgtgt cgtgcaagca tgccatagtc cagttcaccg 42540

tcctcggtca cgatggaatc ttccagagat ttacggtcgg tcaaggcttt ctctaccaga 42600

gcccgctgct cagggttcat acctgccatt gcttcttcca tttgctcagg ggtgatgttg 42660

aaatcagtca ttttatatct ccttttcgat tatttagatt tgcggtcgat tttgaaaata 42720

gcgccgctgt tgcagtgtac agcaccgtct ccgaagaagt caactccgaa atacttgacg 42780

ccttcgttat tctcgcatgt tgaagtcact ttctcaatat cttcggcggt aaagctcgca 42840

gtgatactaa acataaagaa caagcctgca agggccgccc caaggaaaaa ctcagccata 42900

tgccctccta ttacctaatt agccgataac tttaacagag tcgaagccga gcaggcctgc 42960

gtcctccagt tcctcaatag tatcatcaat cccgcaatct gacaaggctt tagctacatc 43020

ttcttccatc tggtagtagt ctcgccacag gcactcattg ataccgatat cccattcaca 43080

ccagaactta taaaccgtca tttgaaacct cctttctcca tcatgaagtc taggcttacc 43140

ggcattggca gaccaaaacc atccttcact tcgtgaatca taacacaacc ccggaagtga 43200

ttgttaccct gatagccttt gtaagcctcg tcgtgaggat agcacgctcc agtcacaatg 43260

cctagccttt gttttccgtc aattgtagga tggatcgccc agtccagaag ctgacggtgg 43320

cccatgacaa acgaatgacc ggcagcctta agcatgttgt tcgctgtgcc acccagaggc 43380

ttcccagtga agtggttttg taggtagtgg acgtaataga tgccatccac ctcgataggc 43440

ttcaggaacg gaacaacctc aaaaccataa tcctgaatac ccagttcctc acttccgacc 43500

gtgccgtaca attcggggtt gtcgttggcc gctcggtctg ctcgctcttc gtggttaccc 43560

agtgtgaaaa tcaaccgagg ttggtacacc tttttccgat tagcccgttg acgctcctgt 43620

agctcccaca gaggcttgaa cagcctgtcc agaccctcac gtccgacttg aacgtcttcg 43680

gcgtatctcc ggccctcaga ggagcgttta cccttatcat aagacgacaa gctggcgagg 43740

tcaaagtgat ccccgatgtg aatcacaaca tctggacgtt tggctacgat atacttaccg 43800

atagcttcca tgtagtccag actgataccc ggcttgcatt gtgtatcaga gattacaaag 43860

tgtttcttgc tgctcattct acttcctccc actcgccctc gcccggttga taatacaaaa 43920

acgcatcaat acgtttgtac aagtccgtat catatagtcc gtttttctgc atcataacca 43980

aggcctcgtc gagcatagag acagcttcga tcaagtcccg aagttctgct atagttttac 44040

taatatcgcg ctgcacctta ttcctcctcg cactcttgac cgtagaggaa tgctgcaatg 44100

tcctcgtata catctgtatc gtagcaatgg acgttatcta gaaggttgag ggctctttct 44160

agaagcttcg ttgcttcctc cagctcctgt ttgatgaaat acgctcgggg gtcagtcatg 44220

ctcattttgc ctccttcagt agtttgatga tatcgtcgta tgtggacgtt ttaatggtga 44280

gtcggacata tccgtctccg atgtggtctg gcacttcgaa cccaataaca tctttcagta 44340

gatcgagcac caactggctt tcgtgcttca gggattcagc aatatcaaca atctcgaaag 44400

ctgattggtt tttaaaagat gctgcttccc agccggccag acgtgcggga gcggccccta 44460

ctatcaaagg aatgttcagg tcaggccacc gttccatatc tcttttagca tagtacgctc 44520

ctagtgtagt actctcatat gcgtttctcc aagctcgggc tgcccctcct ttcgtggtcc 44580

attgccgctt tcctttagga gtggtccaaa gctctttggt ctttttgtgg cgtataacat 44640

acgtagctaa attggtcatg actccaactc ctctcccgtt ttaatgcact ggtaagctcc 44700

cttggcagct tcgatcaaaa cagagtatcc gtttcttaac tcttcttttg cattatcgaa 44760

gaactctaga attccaacga ttagcgcagt tgcaaccggg agccagaaga acagagcaat 44820

ccaagccata acaagggaag cccttacccg gatgggcagc ttccgcataa ttctcaaaaa 44880

cttctctctc acagaggcct ccataatttt gcaaatttaa gaacattcgg ggtctcctct 44940

ttgctcagag gaggtactac taggcaggag ccttcgccat tgcagatggt tctctcatac 45000

ccctcatata cgaaagggat ttgcttgatc ttatcgtact ctttgtcaga aactcgtaca 45060

acgatcttac ggaagctcca ctgtttccac tccacccact taatggcggt ttttgccgct 45120

gcatgcatat acggacggga cggaaggtcc gtcgatggaa cggcccggtc ctgtccgagg 45180

agaaccattg ccatgtctgc ccctaggatg ctatgtgcaa ccagagtcgg caccatgtac 45240

tctggtgcct cgtctcttac tacaatgtaa acccgatcct cgttcatcca ccctccttct 45300

tagacttaag gtattcaatg atctgttctt ttctaagctt tgcgttagat gcaggcttaa 45360

ttccagcttc ctgcaagaag accttatcat accccttctg cttctgtatt gcaatagcct 45420

gtttcacgat atcagcctct tctagggtca gcccgtagcg ctcggcgtat gtcttgatct 45480

catggcacgg tttgcacaag acagccaaat ctcctatgcg gacaaacacc acaccttcca 45540

cgaacgcttg gatatcctct actcgcttca gggagaactc ccccgtcttg tggtcaatct 45600

caacgttagc ttgaggagtc tccttcccgc actgctcgca cacatagccc cacacagtag 45660

ggtgcctaag cttgctcttt tcactgggat tggttatcct aatccttcgg gatttagcaa 45720

actcaagctt gactgggttc ttagaccaga gatagcggcg aatgccgcct ctgacccaat 45780

tcaagaacgc cgattctgtc tcccagatgc ccgggaagtc ctcccaagga cgcgccctta 45840

cgctcattct gcacctccca aatccactcc aagtttagcc aaaacagccc tacagtctac 45900

ccgatccccc gcccaacgtt gcatgtgggc gcagtcacaa tacatttgca tgatatcgat 45960

tgcatcttta gtgtgctctg tgtcgtccca tgctgtgtaa gtgacaggct caggatacca 46020

cgatttgtag gtgtcgtaga ttgcttgcca gcactccttg tccgttgtgc agggattaat 46080

gatgttaaac gctgccactt ctccgaagtc cttcccgatg cacaggtcag caggtcggta 46140

gcagtcaaca gggtctccga atagaagctg gaagtagaag aacttacgtc cctcgcctct 46200

caccttgccc ttagagtccc tgtagagcga tcctaggcct gaaatgaact tgacttgaac 46260

aggctcacgc tcatagttgc aaagccatcc tagggtgcca tctgcgtctt tatcgatggt 46320

tgccccgatc acacgttgag tggtttcgcc tctagagtaa cttacaccgt tcttgtacat 46380

catttcggcc atcaagtcgt ccgcttctcc gggatggatg atggtgtcat acgcttcgat 46440

catatactgc cttagctgac ctagctgaag aggcttaatc tgccctgtac gcttaccctt 46500

gtacctgcca ccacgagtga aggtcttttt acccttggta atctcgtatt ctttcgggag 46560

tgggatggcg tcacggaagt tatcagggcc tgataggaga atcttgaact gcttgcaccc 46620

agcttggcgg cagattcggt cgatcatggt cttaacaaca gagataccgt aggacacgtg 46680

cttggggtct tgatggtcct caatttcgaa gttgtcttcg ttgaatccct cttcgtcttt 46740

gttattctcc cgccaagtcg tccgattctt ccaagtttcc tcttctcctg tcaatttgtt 46800

tctgacaatg atggagcggg tctcgttgac acttgcacac ttaaaggcga taaggtcgcc 46860

gtcgatgagg acgatatcct cttccttttc actcattttt atctccaatg aaaaagggag 46920

ccgaagctcc ccttgggtta gaactgtaca acctgaatgt cccactctgt ttgtagagtg 46980

tcagggttta ccttgttagg aagagcaaca taagccggct ctcctttaat gatacactcc 47040

gcagcgttct tcaaatcttt gaagagattt cgctcccaca ggtcaccgct gaactggttg 47100

tttactgtgg ggccttttag aatgtactca ggtactcgaa caataaccaa actactcatc 47160

attagtcctc acttattccg gaagtgcttt ttaaagctaa agttcagttt agtacgatcg 47220

ccgaatgcgt aaatcagacc tacaattaca agaaacagta cgagaatcga caatacaatt 47280

acaagaggaa tccagatggg catgagtgcc acagtccaag agatagcgag aactccggca 47340

gatttcagaa cgcacaggac gattgctgcg agaaggaaga tgggaccaaa attgatttgc 47400

atgtgttgcc tcctttgaaa ttttgaaagg aaggggccga agccccttgt cttaccaccg 47460

ccctttagaa cggaatgtca tcatctacgt tggtttgaac cggctccact ttaggtgcag 47520

cttgggtctg cggcttatct tcagcttgct cttcgctgtc gccactggag cggttaccac 47580

cgcgtgcagc ttcaacggct tcgatcatcg ccttgatatc cgaaccttcg tagttcttgg 47640

ctcgcttgat ggtgttgatg acgttagcac gcagttgctt gactgcttct tcgtcgttct 47700

cgccatacag gttgacaccg tagatgtact tctcgtccag ctcgggaacc ggaacacctt 47760

caggaaccat accgaccaga ctgatatcct cagtgaggaa tttcatatcg ttcttgcccg 47820

gcttcatcca cactcgcact tggaactgag ccaccttgcc cagaagctcg cctacgcggt 47880

ccttggtgaa gagttcgtct tcgttgatga cgccagtagc cacagcaagt ttgtgcaaca 47940

tggagttctt ggcgaacgcc catttgccat tcgggtgctt cacttccttg atggtaaacg 48000

gacggccaac aatcttagtc ttctgacccg gcagagtaaa gtcgccgttg tacaggacac 48060

gaagcggaga cttgttgctg ttaccggtca ggtacaggct cttgtctacc tcgatctgag 48120

ggaagtccac agtgatcgcg atttgttgca tatcgcgctg cggccagcac ttcagacgta 48180

caggcttgcg agtctgcttg tccagtccat ccttgaagta agtgttcgga tattccttga 48240

tgatttggat ttcatcttcg gcagtgccag tgaacacaac ctctgcatcg ttctgctctt 48300

gctcgccgag atcgatgata ccggagatat aacccgggat cgaacgcgct ttagcggcag 48360

tgccagcagc ttcaactacg tggttgttca gagcatccca atcgacttcc tttcggacgc 48420

cgccgttgcc ggattgagtg ttggtggtta cgttcagttt aaaagccatt tcatttctcc 48480

tatttaagta ccctagactg ggcgagtgtg tcagccactt gactgacgat cataccggac 48540

attcccggat tctgcaagag gagaagcgcg ttaacgcacc tcctctcctt tgattacttt 48600

tacaatcgag actccctcag ccttgccacc ccaccagcgc ttggtggctc gggcttcttc 48660

gcgagtgagg cagaatttaa gaggactccc gtctgcatcg agaacagcat aacccattac 48720

agcaggcttt gcaacctttt ctttcttctc ttcgtcaacc ggagcttcaa ccttgccctc 48780

cgcctcttct ttaaactctt ccagtaccag ctcagggtag agggtctgga actgcggctt 48840

gtcagaggtt tcaccgccaa tgtcaacgcg tcctttaaaa gtcatgcaga acgggctctc 48900

cccttcgtgc atgctcttca gccagcacgg ctcgaaacct acgtacaagg ctggctccga 48960

aggaaccaag taatggccga tctgtgcgct gtgcttggca acgttcttaa cgtaatactt 49020

cttgcccgga actgctacaa tttcgaagcg gtcgttgtca gcgagcatcg attggtagtc 49080

tccaagtcca tcattgacgt ctcggtcaat ggaccaatga agggagcaga accggatttc 49140

cccttccggg agaccgatat cgcgtccatc tactgcgact gcgtatgcgc ctacatatcg 49200

ggcttccttt ggccagccaa cggtgacgtc acatcccact tgagtgagtt tgacgatagt 49260

cccgggaata acaccagaca tattatttac ctcctttctg tttgtatttg ttagccacgc 49320

ttttgtgggc taacggtgtc tccgatacct cgaagccttt agaggtatag tactcccgat 49380

tggccttgtt gtcaacagtg attcctacca tggacgattt gccgtagacg tagagtcgag 49440

ccgtgttcat accatacctc ctattctgtg attaccgtat cactcgctgc tgccttcaac 49500

agccttcaga gtttctttct tagcccgctt aaccagagct tcgagcagtt cagtagattc 49560

gctgagaacc tgctgagtag cgtctgcttg atagaggctc tgtgcagcgt acccgccgac 49620

catccaccat gccgtctcag acgaaggcac cagatggccg attacaatga taggcagaaa 49680

cagcttgaac gtcaagggcc gtaggtgatt tttgagtgtg gctttaactg ccgaattctc 49740

tcggccacca atggatgcca agtaaacgaa ccctcctgca acccatgcga tgatggtgac 49800

gatcagggca aagctaccag ccgacgacaa ggctgggaat acggtcatta ccagatagat 49860

aatgaaagcc agagtcattg tgtttctcct tgtatgttgt gaatatgtgg ccattgtata 49920

ggaggcggag gggcctgtca agcccctttc ctcgcttaat gccagtttta gtggcaatcc 49980

ttccaagatc gaccaacgat gtagccagcg gtcagctcga taggcaccag aggagtagag 50040

tagaactctc ccgcagctct cacggccttc gtggcaagtt ctccagcgcg gcagtaagct 50100

acaaacaccc cgccttcacc tttgctgtag tgtgcgatat cactccacac cttgccagta 50160

ctagccaatt gttcgtcctt gtaagcctga gctagagctt tcagcttctt ctccgcctcc 50220

tcctgttcct tttctccttc tggcttaccg cctacaagag accaagggaa catcttgaag 50280

ctgaccgact tagctgtaac ttccaactgc gcttcgtcgt ggtacgcgat catttgttgg 50340

cagaattccg gcttgttctt ccagtcttct ttgaaaaagt caaccttcag accttctttc 50400

tccagtagta catcatgaat caccatagcc cgcttagcac agataacacc gccactctgg 50460

aacaagctgt tcaggatagc gtgagccgaa cgagtaggga ctcggcgacc gtccactcca 50520

atgatatact tcttgccatt ggcttcccat tgcttggtga ggtcgtcctt cagagctttc 50580

aatgggaatg ctgcatccca gaacgcatcg aataccagtt gacccgtttg caaatcggca 50640

ccaatggttt ttgccacttt accagcttgg gcaccgtacg tacacccgta ttttacgttt 50700

ttcgcaggag atcggttaaa ttgacgacca atgatctcag aaatacgctt agccatcatt 50760

gtgtgaacgt caaatggctt ctccatcata agagatttgc aatattcctt tgtcggatcg 50820

tactgatcgc agtaggccga ctctttacgt gcttccaagg aatcgaagtc gtatccaatt 50880

tggaagaacc catctccaac cccaaacaac tctcgaagct cagccccgta caacgaggtg 50940

atccgaggaa cgttagcaac cttccggtgc ttcatccggc tagtagcagc cccgcaagtg 51000

tcagcaggcg tgggaatcct gccgtcctga cgaatgcttg caaggtaacc tttttccggc 51060

tcctctccat cctccatttc gtcccagtcc atgccgccac cgaggattgc gcttcgccgg 51120

tgcttaaagg tcaggtactc aatcacatcc ttcgcgaacg gaaactgttc tgcaatgcgt 51180

tcgaggtctg gacacatttc tttctcttgg ccctttgtga agctagggtt agtgagaacc 51240

ttacagctcc ccttgcttgc acgatcaatc aacctctgac gtacatacgc gcgggtgcta 51300

cgaggggtga tcttcatgta atccccgaac ccgtccagcc gctcttcctt gtacgctgtg 51360

ctatacgtta catccagcca gcgatcgatc ttagcaacga tgttttccat cggaagcttg 51420

atcttcttct gatccacagt cagatcagtc tctttccact ctgatggaat ccaaccaaac 51480

tcacccataa gccattgctt gatgtgagtt gtgtccccga tcttagcagg catttccgtc 51540

ttcagcggta cgtcagcggg aagcggaagc ttgtactcct tgccttcgaa gatcatcgtc 51600

cggtcgtcac cctctttcaa ctgagcccca atccgctcaa taaacttctg caagtagctg 51660

gtgatctccc cgttctgctt gaactgccgc ttaggcggtg tgtagtctgc catgaatttc 51720

ttggtagctg gtcgaggagg aagcacaggc tctacccggg cccgacgctc ttccatcttg 51780

gcgtccaagt ctttcacagc tttttcacag agatcaacat tcagaacaaa ccctcggtgc 51840

tcctgatagg tgatgatctc tgcaaccttc ttctccaact gaatcgggga cagccaattc 51900

ttgatgtagt tgtcacggtc ggacagttgg taatcttcgt ggtcctgcaa ccaatgccat 51960

acagcggtgt tcgcctttac gtcatagata cagtagtaga gcatatcggc tgcgaagtcg 52020

aggaatcgaa catccggtgc caaatgctta cggaagtcaa tcttggtgtc accacctgca 52080

cgacgagaca gcttatccag actgtgaccg ccgaacaggt cagggttaca agtcttggag 52140

ataacgaggg tatcgtcgaa taccacttgc ttgcccatcc aagtgtcagc agaccacgta 52200

gtcagaccgc catccctgtg ctctacgccg atagtgaagt ccatatcttc cacaagcttc 52260

atcgacataa ggtcgaagtt aatcatgttg tgagccacta cgcgcttgat cttgcgcttg 52320

agcacatagt tcttgaactc tttcagcggc aggtgaatat actctaccgg agtgtagtcc 52380

ttgagagtca caacttcttc agtgtggagc atggaagacg ctttcggcag tctctcagtg 52440

tactctcgcc cgtcaaagac gtaggtcggc ccatcgtaga atgccagaat attgccaccg 52500

tgctcctcca ctacaatgca gtggtgtttg tagctaggct taatcttgta ggggctggca 52560

gtgtaatcga tagacgtttc atccaacagg cctgtggcct cgatatccca agtcatgttg 52620

gtgaaattgt tatttcccat ccattcgagg tattgctcga ttgtttcaaa tcgttgttgc 52680

atttcctttc ctcctgtaaa cgacgaaagg gatgatacat aagcatcacc cctctgtcaa 52740

ccttagtatt ccgtgtcccg gccttcctct tccttctgag ccatccaagc ctcgtattca 52800

cggcgcttgt cagggtatcg gtccagatag tcgtccaagt cccagattgt atgagtctca 52860

acgtcgtagt accaattgcc catcattcca gtgcggccag tccatcggca cttagagagc 52920

ttcacgttaa tcaggttctt ttcgaactcg ttctccgcgt ccttgttccg ccacatcaga 52980

atgttagctg caccagattt gaagatggac gaagaccctt ggaaatcttc ttcgtacagt 53040

tcagcacctg ccgagttagc cttctgacca ccaccactct tacggacgtg gttgatgttg 53100

atgaacgtca ctcggtggct cttggtcata cccttttgcc agcgcatgaa taccgactgc 53160

tcttcgttgc tcaatccgtc cagaatatcc tgaagcgggt ccagaataat aacacggcac 53220

tcaaactgaa taatcagttg ttcgatttga gccttgaggg ctgctaggct tccgtctcgg 53280

tcatcgatca gatagaagcg tgggcttccg tcttccttgt gccacaagtc gtaccgagcg 53340

tcacggtgcc gatcaaggaa cgccactttc tcttcaacgg tctcgtacaa gtctgccttc 53400

tcgcctacct tacggctcag cagcttgatt gcgtactccc cgcagtccga ttccagagtc 53460

acagtacccg gcatatgagg cgagttataa atccaaaaca gagtgaactc atccactaca 53520

gtagatttac ccatgcccga tgcagcgccg atattcacaa tccggcccaa cgggatgcct 53580

cctgccatag ccttctggag cttcttagcg aacggtggca gaggaatctt ctcaacctgc 53640

acagcttcat acattcgagc gtcgagtgca tcagacccta caataccgct aggggtccat 53700

gtacgggctc gatagaatgc actgacaaac tccgactgac ggccagcttt caaatactcg 53760

ttagggtctt tacggttcat ttccatcatg aacaccttgc ccttcggaag cttatcggct 53820

gccttgtctg ctgcctctcg tcctgcatca tcactgtcga aacagaggat gatttgttcg 53880

aatcggtcta gccactcgta ctgagcctgt agctgtttcc acgccccact ctcaccgata 53940

gtagggctga ccactggagt cggttcataa tcactcttac cgcctgctcg ccggtcatca 54000

tactccttga gcatctggaa tgctgatagc tggtcaacct caccgcctac aataagcacc 54060

ttcttgctgt tgctattctt gaacctgaac tgaccaaaaa gctcgcaatc cttcccggtc 54120

tcgccgatag gctctgagaa gttcttcggc atgatccgct gtttgtagcc agtgagttca 54180

taattagtag tagtaggata tagctgagtt gcaatctcgc cagtctccgg gtcgaaacca 54240

tgtagcactc cgaaatactg gcaagtttcc ttggacaggc cacgataacc acgcggatca 54300

tacgagcatt gctctttaat ttgagcattg atctctgcgt taaattcagt tcccacgaaa 54360

ttccactcct cttcgtcttc tccccgggcg tctctttcct gctcttcaga gagcaaggtg 54420

tacccacacg cccaacaata ggcacctttg tgttccccgg tattacgatc agtaccatag 54480

acctttaggt tgtctccgct gttgtcagcg cctttcttcc ggcattgggg acaagcggtc 54540

ttgtggtcag aatacagatc gataccgtgc atagccgcaa tttgggccac ggtcattttg 54600

gacataaaac ctccttactt cagacaacca cggagtttag catcaccttg cgttcgtgtc 54660

aacccctctc cgagacgtat tggtcccgca ttgcacggtc cataggaaca gccacctgat 54720

agccggagcc cagagtcgtg actgtccatt gatctacagc gttctctgtc aaccactcat 54780

tcatttccga gaacgtgtgg aagtagatgt atagcagatt gtggtttctc agtgtagcct 54840

tcatggcgag tcgccttctc tcatgtggtt gaatggtgag aaatccgcat gatcttcgaa 54900

cggaccgctc cagtaggtgg caaactcgac ctcggtgagc cttccgtcgt tctggtagta 54960

accattctcg taccctccac accgatgaca catgcctcca gtgacttgct gcatccatcc 55020

aagcccaacg tctacaaact cagcttcggt tttctctcca caccatctgc actcgggtcc 55080

gttcattata ccctcctacg cctgcttgga caactcagag cgctcgaaga acaggtgaag 55140

gtactcgata agctctccgc tttcgtcttc gtagctgccc acatcaataa cgcaaccctc 55200

ttcgtctact gcgatgatag tggcaacacg gccccggaag atggtatcag cgtcgtcatt 55260

gatcctcact ttatcaccct tcttcattta atcgctcctt ctgtttatgc ttaggcttcc 55320

gcctgtattt ggttttatcc ctctctaccg agggcctgtt gactgtgttt agaaacttga 55380

acaccgggtt cttcttagcc atagaatact cctgatccac atttactgca acccttatac 55440

cagatgccag tcttatgacg gagcatgaca ggctctccaa gatgcataca ccggtagcag 55500

agtcgtttca tttgtcctcc tataaacgtg tgaagtcttt cagcgggata tggaccataa 55560

cttcttggtc ttgccagtct ccacggtcaa tcctgccacc catgctcatg tagttgacgc 55620

cattggctgt cattttgaca tatcctgtat agtccgtcca ctttaccaca aggaatgcgg 55680

gaatgtcaaa caccctttgc atttcgatcc catggagcca cttcatctgg gacacaaaga 55740

agtcgggata tttctgcatt tcattattcc gagccttaag ctccacaaag ctaacaacct 55800

ttccattccg gcaggctgcc caatccatat agtgggctct aggtagctta tgaagagaac 55860

acttccaccg cccttcaagc tcagaacgga cggcctcctc gaccgcccta tctgatgcag 55920

actcgtaagt ctgtctagcc actcaaacct ccttgaagaa gacgtactcg aaggagttgg 55980

tcataatctg caacttgccc tccatgagtt tgccgtccac gccttcgtct tcatttcccc 56040

agaatcggaa acggcaacgc tcgaaatcaa gctccaagaa gctaggctca agctctcgaa 56100

tatccgggtc aagaacatac tcaggtttga tataaaatcc gagacaagca gtgtccagca 56160

catcccagcc gttccagtgg gaaatgaagt agctctctag cccacgtgct tcaactacaa 56220

tctcatcgcg cttactctga ctcatgctta cacacctcct tcagttttaa tagagaaaga 56280

atctaggaaa tcttggatat agggatcgag ttcgccaact gcctcaataa gccgcatgca 56340

ctgacgctca ttatcttctt cttctaagat tacaacgctc atatacttac gccactggtc 56400

atacaactcc attgttcacc tccagttgaa tcttggtcaa gtacatacct tgcatttcat 56460

ccaagaagta aatgagttct ccctgttcca ccaccgtcct gattagcgcc cctgctacct 56520

catactgtag aatgaggtgt acgttacctt tcctatcgta aatcttctct actacataca 56580

ctacgatagg cggggtttcg tcttgattta cactcacagg cacgtccact tctacgactt 56640

ggccagttcg tagctgctcg acggactcat tcagtcgctg agcgttagcc ggagattcca 56700

taaggtagtc gtggtcacaa ccgaaggcct cgtctagggt gcttacggga gcttttgctg 56760

ccttacatgc gatgtaaaga tcagaagcgt caacaactac aacagccctg tggtcagtgt 56820

aggcgtctag acgtactgca cagcactctt ccttcagtcc cagttcagtc atcacaacgg 56880

tgcctttcaa gcctccggcc agttcgtcgg tgtggaccca actgtcttct acaaaccccg 56940

tagctagcag ttcatgcaaa cgaccatgcg cctgcgccca tcgctttgca catccagtgt 57000

atgtatcgca ataggcctta aggaccaccc gatctcccac ttcaacctta ataatctcgc 57060

tcatgcttta ctccctctgt tgatagctcg ttgcttcttt gcctcaaggt aggcgtcgta 57120

ttgctccgct gtccacgggg gaaggtcatt atccacgctg aacgggttgt cgtcaaccca 57180

tacagagact tttattccaa gattttccat gaatggtcgc tttgccttct gtgccgtgaa 57240

atagatcggg aacccgtagt cccgtagcca ctctaactct ccgctgttgt cagggtggcg 57300

tgcagtgact acgatcacat tgaaagcgcc tccaaagccc ctgaaggtat ccatgacgat 57360

tcggaaggca agagggtaga gactgattgt ctcatcataa tcgaatgcaa ttacaaaagg 57420

ttcgcccata aaacccgcta actgatccat gttattcacg cccctttgct acgtactctg 57480

tcgtaggagg tgctacatgg gtgacaggga ctgcaaccca agcagtcacc tcttcgtgac 57540

agacagggca ttctgcggag tagtatcccg tctctccggg acggcagaat gagaacgaaa 57600

catactcaag ttccgacctc ttagcccgga gaatgctcaa gcagtggtgg cacttagccg 57660

taaacaaatc ctcttgaggg agcgtccctc tcgaaataat ttccataaat cctcctcttt 57720

gtacttcaga tgtttgtgtt tagacatacc agtccggggc ctttctcaca ggaggtgaga 57780

ttggatcagg cttcctctcg atcttctcag gtgactcccc gggccacact tgccagcagt 57840

gccagatggt gcgtcttttg tcattagtgt gatcgtacaa cactccgtcg atgattgcaa 57900

cagcatgccc ctttaccgcg atccagaacc gaccttcagg gttttcctca gcaaactgcc 57960

taagagtctt atccttagca tcctcccggt agagcatccg ggtcttaggg caccaagtgt 58020

ctgcgtagct gaagcgcttc tgagtagccc cttgccggta accgcgcttg cacttcactt 58080

tcaggtgctg gtgtgccttt tcataggtgg tgttccatac gactgcaaag ctcaccacca 58140

tgcagtcgtt ggtttcgcca tacctacgag aagcgtcgaa gaacctaggg taagggtctt 58200

tgaaggtagc tttcgactcc tccacggtta cgcacctacg gttgggaagc aatctgcaag 58260

agcttcgacc tctagctccc ggccatcgtc atcgaagtat ccgcagtcga ttagcgaggc 58320

ctcataccag tcccagttgt caacgccacc agcctccaga gcttggagct tcatacggtc 58380

gcgtaacagg tcgattaggt cttctcggta aataacatgt ttcatttata cctcctctgt 58440

gattgatttg agaatactct acacggattg cagagctttg tcaagctctt gtatgcagat 58500

agatcggtag tggaggcaga cctgtcccta ggcttgccat acggattttt ccgcctaaga 58560

catgcctctg gtctgcctct cgctctcatt atgcagatga gtcggtgagc ggggagcatg 58620

ggctccttat aggctctgag ctttcccctt tcgggtcgtc gctgccctgt tctcagattc 58680

gactcaggat gcgcttgcag ctttcctctt catacgctat actgctggcc ggtgagtgac 58740

gtggacattt ctctccagca ggtcacccac tttaccacgg attattttct tgtcaaggct 58800

attgacacag cagaggaatc cgtccagaat acaacgactt tcaacaaaac aggaggaaac 58860

accgtgaaga tcgatattcg aaacgaggcg gcagaggtca gggagattgt cttcggagat 58920

agtgtagact gtgcttgcag acttgaaaag agcgagtata ctgacgtacc tgtgtacatc 58980

gcagatggct taccggggaa tagtgggagt tggatgccga tccgtgatct tctagatgct 59040

gagaatctgc tactggcctt agagcgagct attgacctcg gctggtttaa caagtaatat 59100

accaattttc aaacaaggag gataacatat gagcaattcc caagaccgcg tactagcccg 59160

agtggtgcgt atcgacgatg tgaagaccca tcctaacgct gacagccttg atctggcgct 59220

tgttggtggc tggcagtgcg taatcaaacg aggtgagtat caggctggtg atctggcaat 59280

ctacattgaa gtggattcca tggtccctat ggacaaccct atctttgaat tcctgcgagg 59340

ccgtaatatg gttgagcggg atggaaaaga atattctcga atcaagacta tgaagcttcg 59400

caaagagctt agccaaggat tgatcgtgcc tgttcatgca gccggcagtc cagagccgaa 59460

agtagacgaa gacgttactg atcgaattgg catcctgaag tatgtaaagc catctgagca 59520

ggcagctatc aatggcggca acaagggtga ccgaactggc acttccaagc tagggttccc 59580

caacttcatc ccgaagacgg atcaggctcg cgtacagaat attcagatgg cttacgatca 59640

ggctcatcag aaggacgaga agtttgaagt gacattcaaa ttggacggtt cgtctctcac 59700

tatctggcac aaggacggag aagtaggcgt ggcgtctcga aatgtcggtt tccgtctaga 59760

agacgagaag atcgggttct tcacggctgt caagaacttc tttaagggtc gtggctggaa 59820

gcgtgtgatt aagaaagact ccaatcactt cactgcgatg gcagaagagc ttaatcttgg 59880

tgaacgtctc cgggagtttg gtctaaatat cgcccttcag ggtgaactgg ttgcaccgaa 59940

tattcaaggc aacttcgagg gagttgacaa accggaatac tatgtgtacg atatctacaa 60000

catttcggag agtcgttact atcttccagc ggagcgtctt gccattctga atacgctgaa 60060

ccagttgggt ggtgagacgg tcaaacatgt gccggtgtac atgtgggacg taggcgttcc 60120

aaaagacatg caactagtaa ttaaaatggc tagtggcgag tctgcgttga acggtaagta 60180

ccgggaaggc ttggttttca aaagcatgag ccgagacttc agcttcaaag ttattagcaa 60240

tgagtatctt ctgaaggagg agtaaatatg gcttactggg gatgcgggga ggttgatcga 60300

gttgtgtgcg aatgttgcgg gaacaacttc cctcccgagg aggttgtaag cgacgacggc 60360

atgctaactt gtgtcgattg ttattttgaa atgttctgca aggagggtga agttaatgag 60420

tagagttaaa gatcatatcg aggaagctca tcgcccagct agccgagaga ccacccactt 60480

gggttctttc gtcttggcca atggcgtaag tggagaaaaa gtaccagcgt ttgttgtagc 60540

catggagact caggaactat ccgctgagga caatctgcca ctcctattat actttggtgc 60600

cggtcatgca ggctggagac catggggaga agtgggcagc acttccctgc gaatcgacca 60660

gagacttatt gataagcgcg attgggaagg gggagctgaa gttgttaatg cgattgatgg 60720

atatgaagat ttcttctcat acattgacga acaagagctt atcaaaaaat tccacgacgc 60780

ttggatggaa tggcctttcc acactgcaat tgttgaagat caagagccga cgttcggtga 60840

actattcgaa cgagagtccg tcttcgagtt tgttagcggt tttaacaaaa tgatgactgg 60900

tcttgaggtt gttaaccgca agtggaacac cgatctggtc acaggcgagc cgattaaacg 60960

tgatgctctg cacatgctga tgctcgttac cactgaaatt gcggaagctg ctgaaggcgt 61020

ccgtaaggat ttgatggacg atcacttgcc agagcggaag atggaagagg tggaactggc 61080

cgacgctatc attcgcatca tggactatgc gaatgtacgg ggtctggatg ttgcaggggc 61140

tatgatcgag aagatcgagt acaacatgac ccgtgcagac cacaagcggg aagctcgcct 61200

tgcagaaggc ggcaagaagg tgtagaatga gcttgcaata tatggtgagt ggtgatgagc 61260

acatcgacga ctatgcaacg tatgagttga gttatcccga tctcaaagcc acgtataacc 61320

gcctgtcgaa gctggacgat tccagcttca aggcttctat gccagaggta ttgcacttct 61380

gctgttttgt agcttggata aaagagattc ctagctctat tttgctggga gacagaggac 61440

tgatacacct tatcgctcac cacattcatg aaggagcgga agacgatggg ccacaggctt 61500

ttaagtctct gaggcatcag ttcaacacac taatgaagtt agattaagga ggttttaaat 61560

ggaattattt gacgttaaga aagggcaggt tgttatggtt ggcagtctgg acatagacat 61620

tccgaggccg accttcgaaa agaccaacgc tcggatgacc ggcgtagttg tcgatattga 61680

cgagggctcg atgcatctga atgttaaagt gctgttcgac aacggggcga tggactgggg 61740

caacgcgtcc gatattaagc tactcaattc cggcccagag gctaacaaag ttcgcaagaa 61800

gctggctaag aaggttatcc agaagcttga tgacctgttc gatgaagtct atcaagttgt 61860

tcgctgaaaa cccatgacaa agagccccgg taagctttcg ctcccggggc ttaattattc 61920

ttgtttttct tttattctcc gctcaagttc gctaatcttc cttttaagcg agtcgagttc 61980

ttcatattgc aaacgggatt gccgatcctg tgtgtacgag gccgagttta tctgatcctg 62040

tagtctgttc agtttgagtt cataccgccg ctcagattcg ctaattcgaa tctcgtaggt 62100

ctggcgtaca gagtcaaacc gagcctcggg agtcatcgct gtgaaagcca gaatagccat 62160

acagacaagg agggagataa acaaagcccc gactgctgaa actagcggcc agtcttgttt 62220

ccttgtcatt atattgtctc ccagttgggt taacgggttg tagttattga gttgctgtat 62280

cccgaccctt gacggcattc ataatcaaaa tcaaataccg gttggtttct tgttgttctc 62340

ttcttacgtt actaatttca ctttcaagtc ggctattgaa tctcgactct agtgcgttaa 62400

gttttgcctc ggataccata tctctttgca tcgcaaagat acgttcttcc tgcttagaaa 62460

gactggaact taggaatcca gaccaagcca ttaggatagc aattagcaga gagataactc 62520

cctgtgtagc taccttgatt aggccttctt tgccgtcttt actttccata cagagccctc 62580

tgctcttctg cccacagctt gatagtctgg agttgactat tgcacatagc agcactgtta 62640

gtgtttagaa tgtaagcttt tgcaagcgct tttactgtat cttgttccac tcggtacacc 62700

ttcacttcat cggttaggct ttccgggggc acaataagga tcgtttggta ctgcggaaca 62760

gaaggctcta tcgagcaagc ttgtaagagc agggtccaaa cgagcaccag tagaaggctc 62820

agagatcgcc tctgtagcat tttctttggt ctcccttgtg gttgggttag gaagggattc 62880

aagctcacac aggtggcctt gcagaagctc ctgtcggctc ttcatttggg tgatgtgtgc 62940

ctcaagtctc ccagtggtga tttcacaccc tgctcgttct ttctggaatt gctcttccat 63000

ctgtcgagac gcagcttgaa gagattggat atcattcttt gcaacggaca aatctccata 63060

caaagagaac gacagatatc caaaccctgc tgccattgcg gcagctacta cgagggcgta 63120

gtttttgagt ttagttagta tcatttccta tggtatcgtc ttctatggtt tccgaaatcg 63180

cttcctccac tttaccagca gttacaaaca ctgccgaaat tcttacaccg acagctacca 63240

gtgtggccac tgcggcgaat acgccgggag gaaggatagg ctcccaaaga tgaaccatgg 63300

cctccagagc cataaaagtt gcagagccta cagcagccca gaacttccag cttttcaata 63360

gcttcttcat taggaacctt cctagattta cttgaaggat ttgtaggctt ctgctagctt 63420

aacatcgtaa ttatttttct tgtaatcagg cccgttatac ctccgggcaa attccgccca 63480

gttcttggat ttcagagctt tgtggattgc aggattgatc ttgatgaagc gtacaaaagt 63540

gtttagttgc gatccttcgc tggcgtactg ggcattgaca aatgcctgaa cactcgcata 63600

acctagtgcc tcccaatgga atcccataat ctggaatagg ccccaagagg cactttgaag 63660

tgcgcagtct ctatccattt tgactgcttt atctagacgt tcgtgctccg cttgtccgcc 63720

gaggtatcct ccagctttag ggttgcaaac gtcgtttatt tcagggtcac gacctagctt 63780

ccttttgaga agcttgaaca tccagtgcct ttcgaataga atcttaggga cgccagaaag 63840

tagaaagccg ctcccacgac tctctacttt agtgacggcc tttacactgg caacatcgac 63900

tccgagatca tcggcagccg attggaagtc ttgctcagtt agagccattt ttgatactcc 63960

tgaattacag ggtggaagtg atagcaaacc agttgaacgg gtaagaccag ttgccccagt 64020

tgcagctaac tctaaacccg gtgttgctcc acatatccgt catgaaagcg cccgggccga 64080

tggagttgtt aaacgtcccc attacggtca cttgaacgtt caagattcgt gctggaacag 64140

ggagttggat attcgtcctt gtgtcatcag acagaccggg accagttccc cattgtagca 64200

gaaggccgtt tggtagtcga gcccatcctc tggaaatatc cgactgactg gactggaagt 64260

ccatagcagt tgtattcgca ggaagacccc atacatcgag cgtagccgct cccttcaacc 64320

ctaggttgtt tctagctgct tggacgttcg tgaggtcgga caggttagcc ccagacgaca 64380

ggaagtctcc tgtatttcta gtagaagctg tgcctagccc taggttggac cgagcggtag 64440

cagtgttagc caatccagca aggttgtctg ccttcatcat aaccgccgca gggtctgttg 64500

tagccatccc agtgaggcct aggttgactc tagcttgtgc cttgttaggt acatctgcta 64560

ggttctgagc gctcctcaag aaataggttt ccggctgggt agcactactt gtaattccaa 64620

gattcgatct tgccagcgcc ttgttaggca catctgccag attgctacct gccttcagga 64680

atgtagaaga aggcatagtc gctgtatccg acagtcctag attagctctt gcggcagtga 64740

cgttagacag accggaaagg ttgtctttct tcttcagata cacctgtgcg gccttgagcg 64800

gactcacagc cttggtatca ttggctccag cttctgtctc gatagtcgta gcaactgcca 64860

taaccccggc tacagtctcg gtcgcaggag gaacgagagt cgatagctgg ctcagcggga 64920

tcgcgtgagt tggctgtgtg gcagtggcga cgctgaaaac attagagccg ttcccgtgct 64980

tcatagcgta ccgggcgtcg ccttcagctt tactatacac acttaggttt tgtcttgcaa 65040

taactacgtt gccaatccca gagagggtgt tgtagtttgt tacatgccga tcaaggttgg 65100

cttgaacctt agctgcctct cccgcatcct caaacgccac tcgccaatat ccggctgtaa 65160

tgttcagagg gtcagttgga atcttgtcgg aatgggttct aaggcactta tagatgatcc 65220

cgttagcgcc ttgagtgtag ctcaggttac cctgatactc tgtcacgcta tcccattcag 65280

ggataccatg ttggttgacg tgagcgttaa acaggtcttg tttatattca atgaagtttg 65340

cagtctggta cggaggaagt tccgtaaccc agcctagccc tactttcaca gcacccgggt 65400

caaccttctg gccattttca gaccagatag cgttgattcc cgtaggcttc ataatgtttg 65460

gcatattatc ttacctcact ccttcggagc ttttggttta ctcttctcgg ctgggaagtt 65520

tttatgctca ggccacttac gtagtgcaga gcggtactta cgccaagcct tctcttttac 65580

ttcgtcttca tcgtcgatag caatttctac ttgacgatca gcccgggtta gctcattatc 65640

tctccacaga cgtgcatcta caacgaggtc tctatctgta acgtcctttg gttcggggat 65700

ttctccgtta tcgacagcac gggcgatggc gtcatatacc agtttggctc tatcgtcgta 65760

atcacaagcg tagaacggaa tccatcccca ctgaggatga tttacctcgc attcgaaccc 65820

cattttgtat tttacaacat ttctgaattc cactataatc tcctctaaaa ggagggccga 65880

agccctctat taagaaattc tctgccacag ggtaatctgg ttaccaccac ctgcaaacgc 65940

tcccatacat ctgtaagtgc ctgctgctgg tagtgcccct gctgtgtagc taatagtcga 66000

ataaggcttc aactggcttc cagctctagt tgtccccggt ccaccagacc ctccagattg 66060

atctcccgcc agaatatact gacctacagc gtcccaagcg gctccggctc ttgcgatatc 66120

gttaggaatt tctcccttct tggcatatac cgaatcaacc cagttggtca gagaaccata 66180

ggctccgtaa gcggctcctg tgatattccc tgctgtattg agcgtagcgc tcccggcgtt 66240

aacgttgcct gtcgcattca cgtttccaga ggcgtttagt gtggtagcat tgactcttcc 66300

agtaacagtc atgtccagac cggtaatcga cactgcggtg gctccgccag caactcggaa 66360

gttcatcgag ttgttagatt gggctcccca gataagaccg agttctgttc cgttcgctct 66420

ctggaacccg atgtgggcgt tgccagtgcc cgatgctcta ctgaagatgt tagagccgta 66480

cccgacgata gtgccatacg cattaatcgc aatagcgctg aatcctccgt ctgcgtctct 66540

ctgaacaaga gagttagcct gcgccgtagt tgtggctgga atctgagccg agttggacag 66600

accagtgatt ctagcggcag gaactgtacc atgagtcatg ttaccattag cgtcgaatcg 66660

gaacagccat ccgttagtgc taccgttgaa tcctccgata gccgtaggtt gtacggtcaa 66720

gtaggggcca ttgttcaagg tgctggggaa gacttgagta tcagagctac cttgagttag 66780

tctgtcgaga cccagacgag acctagcgtc tgtaagagtc gggcccagag agaactggtt 66840

tcctgtgtga aggataactt cgttgccaat ctgtacaact gggtttgtag cgcttctgaa 66900

tacaaactgg agaatgttgt catccactac agcaaggcta atccagtcgt tgtcagattt 66960

gttcctcagt gctagctgag tagtcttatc tgcactcgaa aggttcgctc cgttgatgtg 67020

aagacgtgca accgttcccg cataacttgt atctaggccc ggaagaacaa tgttttggct 67080

tccgtcaaac gccacaccgt tgattgtaac tggggttcta agcttgttag ctgtagttgc 67140

gtttccagac agggtagcag tgattacgcc agcgttgaag ttgccagcag catctctata 67200

gaccagagta tgtgccctgt tagctgggtc tgccgtaatg atagcagagt tagctagtcc 67260

taggaaattt cttgcgtttt catcattagc cgcgttgaca agtcctcttc cgaacgctgt 67320

gatgttgaag gtagccatgg aggtatccgt gttgaagtac ggaaccgtgt tggcagccct 67380

agtcacagtg gacaggttgg acagtacaac agagctttct tgagccgaaa tgtagcttct 67440

agctgcggag gcattagcta cattcaacat tccgcgacca taggccgtag agtcaaacac 67500

gctagctcct gtagcgctaa tgaagaatgg gattccaccc tctcttggtg tcaggcttcc 67560

aagtgcagag ctagatgtgg tgtaatctgc aaaagctcga acccagttgg cactaacttg 67620

tgcagggtcg cttcctgtat gcgtacgaac agccttgtaa acaaatccac ctctagtgca 67680

gaacgatttg ttagcaatgt actcttgtgt actagtccac tctgggatgc ccttctgtag 67740

caaatacgct aggttagtat cgtgaaggtt ccacttccag ttccaccatt ggcgaggaac 67800

cgactgaata ccccatccgc cttgctgctg cgcaggggtc ggggctacca tatccccgcc 67860

tgtggaccaa atattttcaa taggtagttg actgtaatca gccatttata actccttatg 67920

tcactaggtt ggtcagaatt ccaccaacag tgggatcgtt taggtctcca acgccttgtc 67980

ctccggggaa gccttctgta gcaaataccc ggccagcttg gaactcagtg tatgtgtagt 68040

taaccccgat agtcttagga agcaatgcac ctgcaccacc taggtcgaat agaagactac 68100

gctctacgtt cgttagaatc ttaccgatgc cgatacggac agcagcggga gcgtactcct 68160

ctaggaatac ttcaggaacc ccgaatacaa atttataagc ttcgataact tgctctgggg 68220

ttgagtttgt tctgttcttg atgatctttg ctttcaggat catccgatac tcttcgtcgc 68280

tcggctctct ggcgttacca gttggagcac cgaccgagta ccatggagca ccgaccgtag 68340

ggtccgacag gcttccgaaa gaacctgcac gctccgttcc agcgaatcca aaatagttga 68400

aaatatcttg gtacactaga ccgcgtggtc gccctacaat gtctccgata atgtccagtt 68460

gcttcccggt ggctgtgtcc agagaccgga gagtctggag gtcttccagt gcattctgga 68520

tatcaaactt gccagacaat agcacttgca ggaagcgatc aaagatcggc ttctctttaa 68580

actgttcagt gacccgttcc cgggccacct ccagataatc ttctctgtca aattggttga 68640

cagccatgga attctccttc tatcattaca ccgtagttac ggtgatatct gctgcggcga 68700

atgtagccac ttctttgaac ccgatttcaa tgttcgccat gccttgagtt tgtcctttct 68760

tggcaatcgt caggctgtta acttggaagc ccgggatttc catgattgga gcatagaatc 68820

gagaataaat tacatcgtct ccgatcttgt aattctcggt cccgtacgct acaatccttg 68880

gccgtaggat atcttcaatg ttctctggca tcgatcctgt gtttgttaca ctgatcttga 68940

tctcgattgg aacctcgaca gggcgctggt agttgactct atgggtatat cccagactgt 69000

ctaccacctg aacctctgta tctccaacag agccaatccc gaatggcttg ttcagccaaa 69060

tagactgtgc aacctctgta ggaataccgc caagaacgat tggaaggaac gagtgacccg 69120

ggactccatg aactgggtct gggttgtctg tatcgttttc gataatccta acatcctcga 69180

ctccttctac gttcatcagc ccgtcgatta gggactccag aatgttggtg gcttgtacga 69240

atttagagtt tcggaatctc tcgcgaagct catcgtcggt ctcacgcaga cggccagtga 69300

tagctggaac agggttgata acgctgtccc atccggcaat aggaatggag atcgtatcga 69360

tagctagagc ttgctgaggc acagggccaa taacgtcgtc aacagcgata ccaagctttc 69420

taaccttctg aatactcagg ttggaggctt cttcgaaagt cttggtgtta aatgggtcaa 69480

taggtactat gaaaagtata ccattctgtt cgtagcttgt caaggtattt ccgtgatttg 69540

tagctacgtc ggcggccagt cctgctagga tggactcgga tgttactgtg ccagtggcag 69600

gacttgtgta cttgatagac gttgtgttaa tgtcatcaac gtacatctta agctcgtacg 69660

tcaggtcgtt gcctactgtc tgagggaaga ttccaacacc agagcaagct ttaggagtga 69720

ggatcacgcc ttgtagtagg tggaacactc ggcctgtact gctggaaatc atcttcgcgg 69780

gaggcgagtt aataacggtg ttgatgttgc cctctagaac aacctgagcc cgggtcggac 69840

gtgcagcatg ccggctaatt gccgagaagg atactaggtt atccagagcc acgccagttg 69900

cggctgccgg gttgaaggag ttatacacca tctggataac ttcccagata gcttcgtccg 69960

atggagcaac tacgccgatc agacggccta gagcagagtt accagacgtg tcaacttcgt 70020

ctccagttga aactaggtcg gcgaagacct gagaggctac gtttctgtag tctgtcagga 70080

tttggtccaa gtctttaatc gttagacctt ctgctgtaat tccagccata ttaaactcct 70140

atcattctga aagtgatggg aagggattct gtattgtctg tggttctgac tgtgaactcc 70200

atggagtatt cacgtgtgtg agggtcgatc cccgactgcc aagatgtaat ctcaaggacg 70260

ccggggtctc tcataatctc agattggaat atcgcgtcaa cagcgggaag gctcctagac 70320

ttaccaagaa tcctctcgaa gtaggggatg ccgatcctat catccaagaa ccactcacct 70380

aggaatgtgt acagtttgat ctttagtcgc tgagcaacaa tatcaaccat tcgttgagtg 70440

actggagctt caccatttat aaaaaccaag tcatgtgtgt cggggttgac aaacaagtcc 70500

atatgatctc ctttaagctt gtgggacagc agtaacaccg ccgcccggca taacaccacc 70560

gtgtttgtga gtgtggaatg gaataccgtt gaatgtagaa gttccaccag tctgcacgta 70620

gttacctgta tgtgcaatgt tcccgttcca tgttgtatgg gctgcatcga cagtgagtga 70680

agcacacttg acgttgacat tttgagcatt aatgcttata gtcttgcggc tgttgattag 70740

aatatctcca ttggctttga tcctaacttc cgcctcttgt cccgtcatca ggttgtgggc 70800

aatagtaagg tctttggtat catgtggcca actatgtctt gcagggtcgt tcatcgacct 70860

agggaaggtg aacagcccgg gaatggcaat ggcatccgcc atgttgaact tacgaagatc 70920

gtttggagtg tgtggctgtc cagtggcaga gcctttaaac acgtccatag ttctttgaga 70980

gaatacacac aacaccgtat ccccaacatt caatgggaag ctgataagtg tgttagctgt 71040

tcccggcata atgacaggga cagatagaat ctctggttgc tcttccgatg tgccgtcttt 71100

gtacaggtcg ttgaccactg gtctaacatc gacagatagg cgtctaaaat cgtccagaac 71160

gcgcaatacc acacaaggta tggcagtgta ctgcttcgaa ccctctgtgg acgagtgcag 71220

gttcagaagc tcttgtaggc ctgttctcct cattggtcaa tatcctcttg gttaagatcg 71280

gaacaccagc attcagtgta ccattcgttg tctctgtatc cgcccgagat tcggacgttg 71340

ttgattctga aagtgcctga caatctgtcc gactctaccc ggacaagaac cccgggagtt 71400

agctcagggt tgagaaggca tttgaattga acgcccctcc gtctccgctg gtctttcttc 71460

tgtgctgttc cgctggcact ggcgtagaat gggatatcaa tcaggccggt ctctctgttt 71520

acgagaggag ccatttgaac actcttcgtt gttgggccgt taacgtctgt gacgtttaga 71580

acgtttccac tgatgttcca ttcaatattg ttggcttcac acacactgtt tagtgcgtct 71640

ttagccagtc cacgtagttg gtatccatac atgatcgggt tgttgcaatt gaggcctgta 71700

taagctcctc tgtccactcc cggcatttga agacgaattt cttcgataac atcctttaca 71760

gttttgcccg gagatactgt gcccttcagc ttggtctctg ttagtgctgt atatccttca 71820

cctagaataa gctgagtgac ataatcgtta ccactctttc tagtcgaagt ttcaaccacg 71880

ttgccatcaa gcacaacaga agttcccatt tccttataac caacttctag tcggcacgtc 71940

agatactcag tttcaagaat gttcagagtg gcaatagaca gattgtatat ctcaacagtc 72000

gccgagtttc cgctcttctt gttatctgaa cttttagaga tatcaaaccg gaactgaagt 72060

ccctctctgt tcaaacttcc gtcagggtgg aggtttgtaa tctctacacc ttgcccggat 72120

tcagtgttac cgagcgttag tttatacact ctgtcatatt gtttcactct acgtcatcct 72180

ccaagtcaac gttcacgtat agcagttcga aaaactgtgg tacaatgtca cttgcatcgt 72240

gcttatactg tacctgttct aggttgacag gcatcagtag gaagtagcca ttgaaaccta 72300

tatgttgcat ggcgtagtct gccatgatcg gatactgagg aacaaccgcc agtcctgtca 72360

ggatagggtt aagttcctcg tcgaatatat ccatgtgcca ctgccgagag cggctattcc 72420

agtagaattg aaggactctt gagattccct cgatagctac tgcgtatctg tacttccggt 72480

cttggtagag aggaagtgtg tcgatataag tcgtgctcat ttagtctacc tcgcaacctg 72540

agttctaatc tgagcctctt tcatttgact acgcttgaat gtaggagagt cttggctggt 72600

agcattgcta gccccggtat caccttcttt ggtgcctctc ttttctttct tagcggtctt 72660

tctacccttg ttaaccctca cctcgacata acgagtatct gtgaatgtca cagcctcgat 72720

tgtcatacga ggttgaaggc tgctacctgt ctcggcgttt tcagtgaaag acaagtcggt 72780

gaagatgcat ctttcgattt gatcccagat gataccgtct cggaagtcca gcaaactgaa 72840

gacctcagca ttacgctgca tgtcgatcag ttggcgctca atatcataag cgactttatc 72900

acccttttga gggaggacag ttgcagaagg aatctcagga ggaataaact gagtgataac 72960

ttctggaaag attctgctag ggtctagtct agaggttgag ataacagccg ggatggttac 73020

cggctgcgta ttcacgaact ctttgttgtt gatcttgtat gtctgggcct cgttgtcgtt 73080

gataacgggc ctgttcaagt tgaagtctgc gtctgatacg acggctgtaa gagtgaagac 73140

tgggttttct gtagtaatgt ggtctgtgat ttttcctccc gtttcgattg ggttggaact 73200

cacagagcca cggtactgtc ttccgaactc tgtaacagcg tcgaaccaga ttaggtctcc 73260

gttctctctt ttgatagcaa ttgtcattgg gattccttat aacttgccga agctctggag 73320

atttcttcct caagaacgtt actaacgatc tctcgtactc gctcttgcgc ttcagttgtc 73380

atgacgttac cctgagcatc gataccagaa acattgaggt tgaaactaat gtcaatggca 73440

ctcttcttga gggcaccctc catttcgttt ctaacttgac cacgttgcat catataccca 73500

aactggtctg gagtcaagga ctgggttgca cgggtatgag tctctgtctg gatcgcgtca 73560

atgttctgag ctactagcct gtcaccgatg aaggtggcct tgtcttttgc atcttccgga 73620

ttgaacatag cgtacatcgt gtcgatgttc gacattatag tacttctgtc tcctccggct 73680

tcagcaatct tagcgtccct gtacttgccg gctattgcga atctctctac aacactgttg 73740

aagaactcca tgatggcagc aagttctctc atcgtgctga ccagcatttc attgaactct 73800

agacctttga gcgcttcagc tagccccggg acaacagaga agataccgtc gagattgttc 73860

ttcagttcac tggattcact ggctagcttt tcgaatgtct cggcagcttg tacgttgttg 73920

ttcaagaagt ccccgaacac cgaatcaccg ccttccaaga agacaatgaa gtcttccagt 73980

gccaacgcac cccagctaat agcagagatc aacctgccca taggggtcag caaggctagg 74040

gtttgagccg tggtaagagt cagccctgtg ccggtcatgt tgaactgctt ggcaatgttc 74100

tcccactggg aaccaagctc ccctccaata ccgaccagag cgttgacagg cctcataaga 74160

gcttcgaacc cgccaccaag tgcagttacc agaggaagcg cttctttcat gccttgggca 74220

gcaatcttga agaagtcgct cattcctcgg tcgaatcctc cagccgcgaa ggcctcgata 74280

gtgcggttcc atccgaactg gaatcggccc tgctgagcgg ctgtaccttg catagccgcg 74340

tccagagcgc cacctttacg agcttcttcc gctagagttc tggcgaatgg gatcagagca 74400

tcactcttaa gcttcccgtc ttccatcgtt ttcatcagtt ccgcgatagt catgtcttga 74460

gacttggcca tcagagctac agcagcgggg aatctttcgg ccaactgacc tttcaattct 74520

tcggacataa tctgcccttt acccatcatc tgctcaaccg ctcggaaaga gcctttcatc 74580

gcttcgccgt caaggcccat cactcgacca tattcagcca tagactggaa gatgctgtca 74640

acttgttcct tgctgaaatc cgatgcctgt ccagcagcca tcatcttaac ataggagctg 74700

ccgagtgctc tgtcgtcgag acctaggcgt tgagagatat cacgtagggt agcttgcagt 74760

tcttgaccac cctgtactcc accaccgaca gcttgcatgg ccagacgttg accttgcagt 74820

tcttggttag cacggttaag ctgcataata gcgaacgcac caccaaggcc gggaataaac 74880

ccgcgtcccc acccggctag ctcgctacca atccccattc ccataccttg acggatatgg 74940

ccggcagtac ctctcgatcc acctgcacca cctcctcgga tggaagactc taggtcaatg 75000

cggattctca cttcgcgtct gatttgctca agaacatctt gaatctctct acgaagatgg 75060

gcacggtcta cagacagcct aatttgaggc gtagcgacag caagaccgtt tagcctaccc 75120

tgcacatccc ttagtgtgtt tcgtgcattc tggagcaatt tagagcggct aatgtctgca 75180

ttcagatgaa ttttaaaacg actctctgtg cgcttcttcc attcatacag cgtacggcta 75240

gcctgagcca agcccagttc aaccggtaga tcgaccttgg tctggccgag agccgtcttt 75300

acgaagtttt tctgatctcg gataccgcta aggtctagcc taacagactt caacctgatc 75360

ggcgttgcat tcagtctttc ttgaagcttc tcagagataa gacgaacagc ggcagcatct 75420

accgcgatgt tcttcaagac taccctttga gtggctgcct tggcaagctc ctctctcaga 75480

gtttttgtat ctagagttag cttaactcca aaccgcttgt tcgtcagctt ggagaattct 75540

cttagccggg cttcgacccc agcgagcttc ttgtcaaaag cagtgagagg ccgggaatcg 75600

actgtaaatt taagttcccc agttagctgg gcaatccttt ctgtaatcat ttcttatcag 75660

cctctgcgtg ttcttttgcc acttgagtga tagtatcgtg aacgtctata acctcaagga 75720

tgttgtacaa atcttctgta gtgaggactg tactcagttc cccatacgtc gttagcttga 75780

gagggtgagt gatagccaca taaatctccc aatgctgcga gaacttctgg tgggcctccc 75840

taaccctccg atcactctga actcgatcag tagtgactga agtctcggac tgcccatccc 75900

cggtctttat accgtcggtg cctctgcgaa accgattaga gaaaaaaccg agccgaagtt 75960

gaactcgatc acgtcttttg tgagtgcgta cagcaggtca tacttgccag cgaattcggt 76020

gtcgaaattg attgccatcg aacctttgaa aactgtcagc atcagattct ggatcaaatt 76080

ctcaacagga accttgtcga tgttatcgaa cagttctttc atggctccag agaagtcgtg 76140

gtcagcttga tacttggcaa gggatggacc cagcaagctg gttagctgct tcagtacgct 76200

gataccgcga gttgctggca gaaggttgat agtgtattct gtcccattgt ggatgacagt 76260

acgttggttt ccgaacataa atactcctta tttatctgag gtagctaaga gcgccttgaa 76320

cagttccaaa cacgtcgaag ccgacttgct ggtttccgcc taccgtgcca tcggtccagt 76380

ccagaacatc aatagtccag accctgttct cgattccatc ggtgaatgtt atgctagggt 76440

agccattgac ataagcgttg tctgtgctca tcatggtgct accgtgggta tccttgacag 76500

acaaggtgat tcgggcggat tgtgttcttc ggtcttgctc tactagcgag aagaatgcat 76560

cattagacac cgaagtctga agaacctcga ttctaatctg cgcagaaagg tctctattga 76620

agacccgggt gtggtgccct ctgattcctc gaaccattgt gtagggtctc gaaagccaag 76680

acatttcgaa agaaactaca ccagtgagag catacccaca aaggacaatt ttaacatcag 76740

atgggctata tgtcaatacc gtgttagcca ttagaagcct ccgaattgcg taagggctgg 76800

cagaagagag cttcccagca ggatagcttg ctcgaccaaa ctttgatcga cgtttccgcc 76860

aatgttcatg gcgacctcag agcaccctag aatccaagtg cgggtctcca gttgtccaga 76920

gaaagatacc ctaggtaggt cttccaccca tgctgtcaga gctaggaagc ttgtagaacc 76980

cttcgtgtcc ttaatcatca gtgggaattt tcccattcgg gttgcaatgt ccacgttgta 77040

aagcatcgaa aggatatcat tggtcggcga ggattgcatg attgtcaact ctactcgcca 77100

gccttggtct ttgttgtaga cccttgcgat ttcaccatcc atcgaaccgt gcttgatgaa 77160

aggcttaata tcttttacaa ttctgataaa ctctccctct gcgtaccctg tcacggaatg 77220

gataccagcg agagttacaa ccacgtcacg cggcgagtag gtggtaatca atttctatcc 77280

tccagaaaca acaaagggag ccacccgtta aggcagctcc ccgtcgatgt ggcataagcg 77340

ctatattaca gccagcgctg gtctagtgtg acacctaggt tacgaagagt atccgcatca 77400

cccgggctca gcttggcgtt accgccgatt agagtttgca ggttgtgtgc atgaataacc 77460

caatcgcgtg tctgcatgct gttcgagaag ttagaacccg gaacaacacc gacataggcg 77520

tcatcactga agtagtcgga acgaccagag ttgtctttaa cgtggattgt gaacaatccc 77580

tcagacgaac gacgggcgtc gtcgtagttg taaagagcag tcaggaagtc gttcgaaggc 77640

gaagtttgtt gaaggctaac agtgagggta gcagacttag aggcgttgta gacgcgagtc 77700

gatgtgttgt cggctccggt atacatcacg taagtgtcag cgtttctttc aatgttaacg 77760

atactgtctt cgctgaatcc gccgagaacc atgctctcac cagttgcagg gtgagtaata 77820

acaatggtaa catcagccgg agagaatgtc gctagacgtt gaacagccat ttagtaatct 77880

ccttatggga aggaccgtcc ttagacggtc acagttccac gaatgtgaac gaagtgaatg 77940

gcaccggcca gacgggcttc aaattcaatg ccttcaaaga tacgctgtgc tctcatgttc 78000

ggagacatgg atagaacatc aggtacgaag actttaggag ccggagcttc agccagacca 78060

ccaacacgaa ttccttcgtt aagctgggct ctgatttccg attccagaat tgttgcacca 78120

acggcgtcgt aagggatttt cttggagtta gccatacgga accacagacg ctcggtcatg 78180

cgggcttcca gccagtccac gaaaatcatt acgtcgatcc attcaccacc ggccatagcg 78240

ccaccgaatg tacggttgac accaccaacg cgctcgaacg tggtaacgtt cttgttcttc 78300

aggttggtag attcggttgg ggttagacgg taagcatcta cggcagctag agctttatgg 78360

gtccatgtgt tgctacccgg ttgctcttgc agttggtatc ctacccaagc acattcaggg 78420

aattgagcgt cagcgtttgg atggtaaatt agagccgtac gttggaaccc ggcagcaacc 78480

agacgagaag caatgtcatt ctctgcggaa gtcttggtgt tagcctgagc agtggctccg 78540

ataaacactt tcttggtgcc ctcgatatga gtagctactg ccatgatatc atcatcggcg 78600

tggctgtcaa tgctcagagc ataccattcg tcgttttctc cttgaactgc tgtgatagca 78660

gccggccaac cttcagtaga aggagcagca gccatagtca ggttagaaga aaccttcagg 78720

ctccagtctc cgttcgaagc gacagtcaga gttccgtctt cgttgtcagt tacggtaacg 78780

ccaacaacag gggtaacatc atatgcagct tttaggccag ttgcaattag ggtggcagtg 78840

tcgtcttgct ggctaacata gctgacaggt agtccgttaa cggtaaggac gtagctttgt 78900

ccttcctgaa caacactgac agagacggta gcagaaggaa cttgtctgcg tccgataact 78960

agactgcgag gcttgagagc ttgaccgaag tagcgaacag cggcaagata tgttggatcg 79020

gattctccga agtcttcagc gacacctttc aggctgttgt agactctggc acgttccgag 79080

aagtttgtgt gagaagcgat gaacagagga acgttgaagt ttgtctgtgc gacagcagca 79140

gtctcacggc tgatctgaat gtcaattaca tcagtaagaa cagtcattcc tgtctcctat 79200

tagtgttcga agtttatgtc gatgtgggac tcaataatgt gacccgggcg accagcgtcg 79260

taataaacgc cgtgaatatc agccccagca gcaaactctt gagttgtggt ttctataaca 79320

gagaatccaa attcacatgt aacttgatag aacatgtaca tatctgtttc tcttagcata 79380

ggaactcgtc ttgtgctagg gagtttgaaa agggatagat taaactttct ttgggcggcg 79440

tgtcctgctg gagtacgcat tgcccgagta atcgccattg ccatgtcaga gacacttgct 79500

gtctctccat acttgccaat cagttcgaat acgactttgg ctgcgaagtc ctgttgaact 79560

tgaatagttc catgcgaagg actaatggga tcgctcatat tggagacatt ttcccttccc 79620

agttcatcca gcctcttaat gtcgatagcc agataagggg tttgcggttc ctgattattc 79680

gtgtaagctt gaattacacg ccagtcgggg aacaactctt tgaccataag atagagggag 79740

ttttctagct ctgtgtggat catgctgtgc tattcctctc tactctcata caaattgctt 79800

tgtaatggtt taggattccc atcgaatagt tgatgacttt cataacttca tacaactctc 79860

cttcccacat gaatctatcg gcactccacc ctcccgggcc ttcctttaac tgtcgaatct 79920

cttcgcctct ggagtacacc ttgaagcaag ccttgcttct gtcggcctct ggaaggatac 79980

gagtgtctgt cgatttttcg ataggttgaa cgttagctgt gattgttact actgtctcaa 80040

ctggaggggt aggtcttcca aaaccgtcgt ctccgggctg ccctcttcta atcaaatcta 80100

gctttacgaa gttggtcatg gagaaggtag gatagagcat tattgcctcc gatggatttg 80160

gaacttgaca gactcaagca tctttccagt atggaataga ggatcgttaa atcctttgta 80220

agctgcccat gccggactgt tagagcccgg atagtcgtca atgttaactt gcatctgctc 80280

tgcaaccatt ttacccaatt tcttcagaag agtattcgtc tgccttccat cgcgtaggac 80340

gttctcgaac gtggacttca ttagtcttgc gtagtggaat tgagatgtga attcttcaaa 80400

ggttggagcc atgaatggcc gggttgggtt gcgagtagta ccgaattcgt tgtaagctgc 80460

aacttgtgca actggtaggt ttccattttc agaaccatat cggtcctcgg ggaagaaacc 80520

aacttctacc gttgttccgt ctagagcctc caccctctta atcaaggatg tgaggcgacg 80580

gcgatctgtt ttaaaactga agcttgccaa gataatcaca ccccatgcta aggtcttgag 80640

gaccggggtt gcactggttg taaagcttct cccgtcttgc aaaaccgatg tagaattccc 80700

gtgttacgtt gtcggggtct aggtcattag ccagcatatc cgatttggag ataccgccag 80760

catatggcac ggccaaagaa attgtaatat tcgggtcttt gagaatgagc tgaagagctt 80820

tcaagtagtt gttgaaatac tcagcaccat atacttcgat atcacctgtg cgttctctca 80880

ttccccttgt caggcggaag agcatcatac gcataagttc aagtgcggct cgtctttcgt 80940

tgccttcgtt cttgtccaac acccattgat aggtttcgtc ggaaagcatc ggaggctccc 81000

acacgtcccc tacaatctct cgtagacggt cgatagggtt gtttgctgga tcaccagtgt 81060

aagccatttt tggtaccctc ctacgacgaa agggaggccg aagcctccct gaagtcttta 81120

cgcattcaca gtgccgcgaa cgatcatctg tggacgacgc agaacgtgga tgaagttgga 81180

ctcggtttcg atcaggatcg aacgacggtc ttcagccata cgctcggtag cgtacagttc 81240

tttacccaga gttcctaggt catcgaaagt gctgcccggt ccgaagtaac gagcgaacag 81300

gtcaccgctg tcggttggga tgaagtaagc atcgccagcc gggatcagac ggttgccgta 81360

cgggtcaacg ccacggtatt cgatgaagtg aatgtttccg aagtagaact cacggaagcg 81420

aaggtccaga ccgcgagcgg ttagacgttc acgccagtat tgaggactag cttggtaagc 81480

tttgtagaac tctttgatgc tcggatggtt aatcagggca gtgaagaact caggagagca 81540

cagggcaacg aagtcaccac ggatcagacc gtcttggctg ttgtcttgca tatggacgaa 81600

gatttcttcg gtcttgccca taacgtcagc ggtagggttg gtcaggtcga agccgacaac 81660

cttacgagtt ttacccatca ggtcgtacca gtcgtactcg atggttccgg ctggggcgta 81720

gctcttacct gttacgatag cgctaacgga agcaacttca gcggtgttag cccagttgcg 81780

acggatggtt tccagcttac gagcacgaac ctcgttcaga gtttcttcgc ggtcagcacc 81840

gaaagcgcgc ttaccttgaa tatcttctgg agtgatggag tcgtcttggt tgaagtgcgg 81900

aatcgcgaag gcacgcagtt gacgacgttg gtcgctagca acttggtgac gagttccacg 81960

gatggcatcc ttaaccagac cgtagcgagt ttcgatctct tcgaactgga cagtgttctg 82020

agtcacacct tcacttgtga agacgcccat gttctcgacg agggtccaag tgttcgggat 82080

gataagcagg ttttcagtct ggtcggcaat gtagaactga ccatcgttta ggtaggaacg 82140

ggtgttagcc atttttaaag ttactcctag tatttattta ctggaacgac cctcgcttac 82200

gcgaaggtaa cgttctcgaa tgcgccttcg atctggtcaa cggcgaagat gttcttggct 82260

ttgaaagctt caaagacggt agctttatca gcgtcgtcaa cgtcagcacc gaagataaca 82320

gccttggagc cgatcttggc aaaaccgcga gcaaggacga cagcgtggct catctgagcg 82380

ttggcatagt cgtgcaggtc ggtaattagg atgtaggtag cgtcggcgat agtagcctta 82440

gttaccagct tgccagtgct gtctaggaca gaaccagttg ttttaacatc aatgtcagcg 82500

acaggcagag tttcgcgagt gtagaacagg ccacggtggc ccatttgttc ttctttcagc 82560

cagttgctaa gacgctgatg atcttttgcg ataatgtttt gagccatttg gaattcctct 82620

agattggcta gatattactt ggagccgtgg cgagctttca ggatagcggc aacaccggcg 82680

acaacagagt cgtcttcttc cccagcgccc gatacacctt tctcgacaag catgtcattt 82740

tggtcagcag cagctttaag aatgcggagc gttcctactg tttgtgcaaa agcttgatcg 82800

tccagaactt ccagagactt gaaagtatct tctacttggt cagccgggag gacttcagcc 82860

agttgctctt tacgggcttt agcgatagca gcggttttct ctgcctcagc agccttgaca 82920

atttcacgaa gagctgtaag ctctacatta gcggcgtcta gttgcttctg aacttctgca 82980

acagcagcga caccagcagc tttttcaact gcaatagcgt cagctagagc ttctaggtgg 83040

gccgatttca ggatagtttc catgttggta atctccttgg atggattttc attggattgc 83100

tcggagacta cctccagagc cttttcaagc agttcctgat cctgtttaag ggaaagggtc 83160

tgctcagggg taagctcgga gagagcttga ttcaggtcag ccgatttata aaccgactta 83220

agaatggaca tagactcgac ccgctggtcg atccagtctt tgtgatctag ttctggatac 83280

tccgttctgc catatcccat ggcaacagag agaacttcgg catcttcata atacatcccg 83340

aagaacttac gaaggaactc agggaactgc attgtgactg taacttcagc agctttatcg 83400

ataagctctt cagttacgcc attggtagct tttgtgatta gagtagtgtg cccgttggct 83460

gcaccacctt ggagcttact gaccagagcg acatgagcac cttcgtgttc aaacttgata 83520

tcggtcagtt tacgttttgc ttttacggtc attcgatatc ctcgactgta gctctggcac 83580

cgatagagac gcctgtgaat tcaccagatt taacgccttc ccatagtgca tcggctactt 83640

cagagccttc agggaagtgc caccactgga gccatgtacc tttcttgatc tctcttccgt 83700

cgtcagttgt gaatgcggat ggagcgatga aggactggac aatctcagct tcctgtgttt 83760

caatcttgtg gaagatatta gctacgttgc aatgagtatt gaagttgatg caagctttct 83820

ctacttcctc ttcggagtag gtgtctgcgt gtaggtcagt cgtggagtcg ccttcttggg 83880

gctcaagaac gacaaacaaa gccatcctct tttcattgtc tagggacttt gtgacctcta 83940

cagccggggc ttcttccgcc ttggcgggag ccccgaaagc attgctgaga gcttcaataa 84000

cactttcaag agtcagcatt ctgtctcctt atgcgttatt tgcagcggcg ttgtctctac 84060

cggctgcgga tgttgatgca ccattaccta ctccagcttt acctgcttga gactgaccac 84120

ccatgatatt atccacatca acaggctcgt cgtctggtcg aggcttaatc ttgagagctt 84180

tgcgaatctt gttgtaaacc tcacggtcac gttcaagacc accgacagaa ccgattctct 84240

gaatcccttt cgagaactct tcaagatcga tttcatctag ctcaccgtat tgcagcttcg 84300

gcaagtctgg actataatca ccgttcagtg cgaacagcgt aggaatgagt ttattatcta 84360

gtacgtcttt gatttcgcgg agccgcgatt caatcgccat agccatgatg ttagtcttgg 84420

caccagctag agagtaagag ccaacttggt cctgacccat cttcagcata tcggcaaaca 84480

aggcttgcag aattttgttg tcccagcgac gaatgatggc atctgtatca tacatcttac 84540

tgccctgcga agaggtcagt tcgaatttga acagaggttg tcggctttct gggtcaaacg 84600

cttgaggaag gataagtcca gattgttcat tcatttgaat gttacgaatt actcgcttgt 84660

agtattcgta gactgccctc tcagcttctg tagcatcttc ggacatatat cgaggaggca 84720

gatagagcgt aggcataccg ttcatatcgc gtgttacgcc aacggactct tgctcttcaa 84780

tctctactcg atatctccaa gcgttatagc acgcacggag agggctgtta ccttcaggac 84840

tgtcacgttt agcatcgacc cggaaaagaa gcatcttctt agctggaatc tctacagttc 84900

cgtccttatt acctaggttg acgtaacggt cgccattgac aacattggca agactttgct 84960

cacaccctag gaagtttcta ccgtcttcag agtacaacca tttggtgata gtagactgag 85020

agcggattgg caacttttta ataccgaatt tgttgtcatt gtacttcgat acagggtatc 85080

cttcacgtcg cttaaacacc atttcatgta cggagaatcc gtaggtgtag aagctagtga 85140

cctctttgat gaaggagtac caactgtgtt ccatgtcatg catgcactct ttcaggaatt 85200

taccccgggc cttcatagcc tcgtcggggt caactcctag atcaacttcc cagtcaaccc 85260

tgctcatcat catttcgaag agatcgagtg ctgatttgat ggtagggtct tccgccatca 85320

tttggaatgt tctgcatgcc ctagggaacc gaagttctgg tcggcgttct tccagaatag 85380

taccattgac ttgtttaagg cctgtactac caatctcgcc taggcgaagt cgcgggatac 85440

ccggtgctgt cttttcagtc tcggccatcg ccgcctccta ttaatttagt ttgaatttgt 85500

tggattgagt catatcaggc ggtaggaaat cagggataat caagctgcca agggatagag 85560

cattaaatgc atctgatgta gcgtcaactt ggtcatcttt gatagcctta cttccgtcga 85620

atcgctcaag ttcatcgaag taatcttcgt tccaatctcc cgcaacgact tgcactgatt 85680

tagtttcagc tacagatacg aacggggcga atcgagtaac tttcgatttg ttagttgtct 85740

tcggtctggc gtggaatcca tgatccgcta ggtctctaat tagctgggca gcataagctt 85800

taccagcagc ccccgggtca catgggattt gaatttcaac gtcttgaccg tcttgcttgg 85860

ctgtttctag aatgaggtcg aaaacacctc cgaacaacct acgctctctg acaacatcct 85920

cgacagtgaa caagccttct tttgtacggc tcatcagcac gccggctgtc cagtcggggt 85980

ttctgttggt ttcggattca atgctaccgc tgatgtccca tgcacgtacc cttttaagca 86040

cccgtagagg cggatgggga acgatttggc accagtccct tgtgaagtat ccagctccct 86100

cttctctagc gtgccagcta ccgtctagga gcttttcacg ttctacccta ggaagggctt 86160

caaggttagc tacatactca gggtcgttga gcatcaagat tgggttatcg aaacagttag 86220

ccgcgatgaa tttgaagctg ataggcttca cctgatcttc atcgtcaggc gctagactgg 86280

gattaccatg aagggcaatg cactcttcct tggtgtctcc ccagaacatc ttaccgcctt 86340

tgcgtacaaa ccagcgagtt acgccggagc gctcagggat cgggattcct gtgtcagggt 86400

ctagccacca ttcaatccat ttacgaagga acgaattgta atcagggtta catgtaatct 86460

tcatgtgcgg aacaaccgag catttcgggt tacgcatacg agagataagg tatgtcacca 86520

tgccttctgt gaactgctga ccttcgtcga ctaggaactt actaacctcc caaccttgga 86580

aggagtcttt gtcagattct tgttcgaagt gtcgaaggaa aataactgca ccagaagaga 86640

acacaaactt gccgtctttg tctttccact tgactttagg gtcaacgagc ttgaataggt 86700

ctgttgcttt ttcaagcaga ccgcccgggc ctttaatctg aggagtagta cgacgagtca 86760

ttacacctcg gaagttagga tcatggatat atttcatgaa gtccattaca ccgagataag 86820

acttaccagc accagcagca ccaccgaata cagtgatctt tgcatcgctg tctgtgaact 86880

ctttctgttt gaaagatttt ggaccaataa catgagggct gagatcgaaa ttctctctgg 86940

cctctttaag cgctagcgcg attggtccga gttttttatc aggaacctta gtgttttcca 87000

tgctacaacc ttatagagtg tgaaccctat cgggagcttg cgggttcttt gatagtgaat 87060

tggtgcccca ttcctgactt gaacagaatt cgccgggtta caaagccggt gcatcaccat 87120

aaatgcttaa agggcaggga atagagcctg tgagacggga aaggaggagg aaaccgttca 87180

gcacaggcga ggaggaaaga gtgtcatgtt gtggaatcga accacctatc gctctgctgc 87240

gagagcccgc tattctcgct ttactggctt acgatccaga gagcctaaaa gcgtcaacgc 87300

gatataccat tgcacgacat tggttggagg cggggactcg aaccccaaca atcgggtcac 87360

aaccgaaagg cagcatccgg gctacacaat ctccacacga ataatgccaa gccggctgaa 87420

agcacatacg gaggaatgtt ccggcttgga cttgattgga gcagttaccg gggatcgaac 87480

ccggaacacc agattgaaag tctggcgatt taaccaatta atctataact gctttatttg 87540

gtgcatctgc ttggtaacga tccaagtcct ctggaatttc aacccagcgc ttctacctag 87600

ttagcttcag acgcgtatac gtagaactca catcttctcc cgggcgttac tccgggttac 87660

tgaggtctac gacttttggt gcgggcttag ggattcgaac cctagtctct tgggtggaag 87720

ccaagaataa taaccagact atacgaaacc cgctttaaat tggtcacccc ggaaggattt 87780

gaaccttcga ccttcggatt ccaaatccga cactctaacc agactgagct acgaggagat 87840

aataaggccc gtatcgggcg ttcgactctg cttctaaagc gaagagtctc ttttggaaat 87900

tggctccgta cctaggaatc gaacctagcg cgtttcggtt aacagccgag ccccatcgcc 87960

ttgatgggcc gtacggaata aaactctgga gcctcgtatc ggatttgaac cgatgtatgg 88020

cagatttgca atctgctgcc tgatcctctc ggccaacgag ggcaataaac taggaggttt 88080

ttaacccggg cctctccggg agatactgga tcacctccta tggcttacag catctaattc 88140

aaatctagga gctataacgg gcccgagccg gacgatttat actggctgcc agtagcttat 88200

cacagctact caattacccg tgccagctta ggcgcttccc cttaccgaga taggtagcga 88260

cactgaaccc accacttagg gttcctcgga gtgcaatttg gaagcgagag gtggaatcga 88320

accacttgta ttcggttatg agccgagtga cagcgccagc cagtcttaac gctctcgcta 88380

tttggtggtc gaaggaagga attgaacctc tagcctatgg cgtccgggtt acagccggat 88440

gatagtagcc atctatctct acaacgacct tgttggcgac ccatgtcgga ttcgaaccga 88500

cgaccttgcc ctagacaggg gcttgctcta gtccagactg agctaatggg cctttaaatt 88560

ctggtagcca gagaaggact tgaaccttca accacccgat tatcggtcgg gtgctctacc 88620

aattgagcta cccggctaca ttgtttgtta ctctatgtac ctatattatc atgaatttct 88680

tgtttgtcaa ccccttcatg ataactttta tagcgccggg gaggtcggaa agcagcgcta 88740

ccttcgtcac gtcccctagc tctctcacta tttggccgcc atagaccggc cttaagtgac 88800

cgggtaggga ctcgaaccct aaccaaatag caaaagagat tcttaatttg gtggccccgg 88860

agggacttga accctcaatc ctctcggcgt cagtttctaa gactgatgtg tataccaatt 88920

ccaccacagg gccttaattt ggcagcgagt aaaggagtcg aaccctcacc gttcatcaca 88980

gcggcctagt tttctagact agttcccgac ctactcaggg ctactcgcta agtatttctt 89040

catctgataa gtgtattaaa acatgaaaaa ttcagtttgt caacacttaa aatcacttat 89100

tttggcagtg agcagtggtc tcgatccaca tccgcgttag cagacgtact ccttagcagg 89160

gagcctcaga acctatctga tttactcact aaatttggtt gaacgggtga gactcgaact 89220

caccgccatc cgacttaaaa agacatgtgt gcagacgcta aactacaatc tcatatcctt 89280

caataccagc atgcagcttc ctgtggcagt ttgaacacag gatggcacac tttctaatct 89340

cttctttcag cctgagtctg ctgtacgaaa caagcttcac gacctcggta tctttttgtg 89400

tttggtcgat atggtgaaag tctaaagcaa caggctcatt ctctccacaa aacctacaac 89460

caaacattct tctcaacctt cccaagaact ttctgttgcg tgcaatagta tccttgttcg 89520

attgtagtat cttggaccta cgtttaggta gacgcttata cgtgtctgca ttgatggcag 89580

aataacattc cttacagtat gtctgtaaac catccttctt tcttttgttt ctggagaagt 89640

cttcaactgg cttctcgatt gtgcagcgag ggcacagctt catatctcct ccttaaaagc 89700

ccgatgccta tccactttag cttccgtcca tcttaaattt ggcgctcgta ctaggactcg 89760

aacctagacc ttacggctta gaagaccgtt gtgcatccat tacactatac gagcaatttg 89820

tgtcactata caggagcctt actctcctgt caacaccaat tggagagaat accgagtctc 89880

taaagaacat cggtcgctag cagcatagcc tagctctccg gctgcgaact tggtcccaga 89940

aggattcaag ttccatataa tttggcgggc cgactccgat tcgaacagag aatacgagga 90000

tttggaatcc agtaggttgc caattacgct caccgaccct taattctgga ggacacttat 90060

tactcgctgt cctctgagcg agacacattc cttgtcgggt gaatgttctt ttactacatt 90120

tggcagccac tgatgggatc gaaccatcgt ctccagattc aaagtctggc gtcctaccat 90180

tgaacgaagc ggcaattaat caaaacgcga gaacgactcc gggagtcttg tcctcttgag 90240

attcctcatt atctcctgat tctgttcctt ttgcaagctc ttttttgagt gcaatgtcag 90300

ctttgtagta agcgacgaac atgtcagaaa gctctttctt gagcttgatt ctcatctgta 90360

gagagacctc tgggttgtca gattgctctt ggaggaatct gatgttctct gcaaagctct 90420

tctttagaag tctaagccct gctttcaggt ctgtctgaat gataccccgg gcttctagct 90480

cggcattcgt agggcgaccg cccttgtttc tttcttgagt catattaaat cctctgaagc 90540

tttgtaagcc ccatatactc cgattatagc acgaaatctc ctgttgtcaa gcccttaaag 90600

aattattcgc accgtaatct actatttata catattgtca gagtacgcgc actctatata 90660

caatgtcagt ccccggtagg gagaggagcg gtaacccctt cgcttggata ccaaccacca 90720

aaatactcat gatccagtgt aggattgaga gcaaacctac ggcaagagga gcagagatcg 90780

tcctccttac cagtatgttt attgatgact ggagtctcaa acgagagttc gctatcacag 90840

cacttacatc tcatagaaat tccttatggg ttgattgatg gattatctgt ggtgggtatt 90900

ctgtgtatgt gtatcaggag aacaccccat gcagatagat tcggctaggg aaggatagag 90960

tcgtcccaag gctcgccata cagaatttaa tctgcctaag acgtgccttg gctctatcca 91020

attcctctta ctcatgcaga tgagttcggt gaggggcagc atgtaggctg catcggattg 91080

ctgtcattcc gctttcgcgt cgtctccacc ctttaacagc attcgagttc aggttgccct 91140

tgtggattcc tcttcatagg cgctaggcgt gggccggtga gtgctttcag acatatctct 91200

cccacgctga atattgataa taccacagat ttcctctatg tcaagcctct accagaacaa 91260

aatgggttga cattcctgag aaacgtggta ttattgtaaa acactaaccc gcaaggagta 91320

aaaagaatat gcaagttatc aatatcgtag aatacaaccc tatcgtctgg cttcgctcga 91380

tttacgccca catgaaggaa ggtttccgag tcgtccgctc catcgaaggc taccctgtgt 91440

acggcaccct ctgtcaagta cagatgttcc gtcaagaggg cgtttctgag gaaatcgaaa 91500

aagagcttga cggcaatgaa gtcattgtgc aagaatggga cgttttcagc cttctgatgg 91560

gcatgcaaaa tgctttcgat caaggtttcc gcattgatct cgaagaccct catttcgcca 91620

atatgaattt cgctctcaag tctgtcaaga tggtccgaga aggcgcagag aagcagactg 91680

aggaaaagtc tacagaagta gctaaggaag ttcctgtaga ggaagtccaa gagactgaat 91740

cggaagataa accaaaaacc cgccgtcgtc gtactacaaa ggagaaaagt gaatgaagtt 91800

taacaaagca caacgtgagt ttttcaaaga ctacgcaatt cagactgtaa agtctaccct 91860

gatgcaggtt gctgcacaga atggcctaga gatcaatcag gaagctctgg atcgcgctat 91920

ggaagtcgta aacctcgaac ctctggcgga agcagccggc gctgcgttct tggaacgagt 91980

agactttaaa accctcgtta aggttgacaa gtttgtacgc actgaagagt tcctgagtgt 92040

agctcaggct ggtgcagaag ttggtgaagc tgtcagcgga gagctgagca caatcctcct 92100

tccattcttt gcaatgctgg gctctggcca gaaagaaagc gaggagtgat tcagtaataa 92160

gggccctttc gggcccat 92178

Claims (5)

1. A high-lytic Pseudomonas Aeruginosa bacteriophage (Pseudomonas Aeruginosa phase) RDP-PA-20001 is characterized in that the head of the bacteriophage is icosahedron and is three-dimensionally symmetrical through electron microscope observation, the head is 60.865nm long, the tail of the bacteriophage is 183.254nm long, and the bacteriophage is Siphoviridae (Caudovirales); the gene has total length of 92178bp, no virulence gene and lysogen gene, and the preservation number of CGMCC No. 21410.

2. Use of the high lytic Pseudomonas Aeruginosa bacteriophage (Pseudomonas Aeruginosa phase) RDP-PA-20001 of claim 1 in the preparation of a formulation against Pseudomonas Aeruginosa.

3. A fungicidal composition for the control of Pseudomonas Aeruginosa comprising an effective amount of Pseudomonas Aeruginosa bacteriophage (Pseudomonas Aeruginosa phase) RDP-PA-20001 according to claim 1; after the bacteriophage RDP-PA-20001 is added into the bactericidal composition, the bactericidal composition has obvious cracking effect within 1 hour.

4. The bactericidal composition for the control of pseudomonas aeruginosa according to claim 3, further comprising an antibiotic for combating pseudomonas aeruginosa.

5. A cleaning or disinfecting agent comprising Pseudomonas Aeruginosa bacteriophage (Pseudomonas Aeruginosa phase) RDP-PA-20001 according to claim 1.

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