CN112236236A

CN112236236A - Centrifuge system for separating cells in a suspension

Info

Publication number: CN112236236A
Application number: CN201980038216.8A
Authority: CN
Inventors: S·凯斯勒; T·马罗
Original assignee: New Medix
Current assignee: New Medix; Pneumatic Scale Corp
Priority date: 2018-06-08
Filing date: 2019-06-06
Publication date: 2021-01-15
Also published as: KR102568314B1; KR102568313B1; JP7336467B2; KR20220039841A; MX2022003223A; WO2019236895A1; MX2020011898A; CA3102600A1; AU2022204733A1; CN115254458A; JP2021526957A; AU2019280856B2; EP3801920A1; AU2022204733B2; EP3801920A4; BR112020025083A2; AU2019280856A1; CA3102600C; KR20210018796A

Abstract

An apparatus for separating cell suspension material into a centrate and a concentrate includes a single-use configuration (178, 240, 250) releasably positionable in a cavity in a solid wall rotatable centrifuge drum (172). The drum and portions of the single-use construction rotate about an axis (174). A stationary inlet feed tube (184), a centrate discharge tube (212) and a concentrate discharge tube (230) extend along the axis of the rotating single-use configuration. A centrifuge centripetal pump (208) is fluidly connected to the centrifuge discharge tube. A concentrate radial pump (216) is fluidly connected to the concentrate discharge pipe. A controller (274) operates in response to sensors (264, 270) in the respective centrifuge and concentrate discharge lines (262, 268) to control the flow rates of the concentrate pump (272) and the centrifuge pump (266) to produce an output stream of cell concentrate and substantially cell-free centrifuge.

Description

Centrifuge system for separating cells in a suspension

Technical Field

The present disclosure relates to centrifugal processing of materials. Exemplary embodiments relate to an apparatus for separating cells in a suspension by centrifugation.

Background

Devices and methods for centrifuging cells in suspension are useful in a number of technical environments. Such systems may benefit from improvements.

Disclosure of Invention

Exemplary embodiments described herein include apparatus and methods for centrifuging cells in large scale cell cultures having high cell concentrations using pre-sterilized, single-use fluid path components. The exemplary centrifuges discussed herein may be solid wall centrifuges using pre-sterilized, single-use components, and may be capable of processing cell suspensions having high cell concentrations.

Exemplary embodiments use rotationally fixed feed and discharge members. The single-use component includes a flexible membrane mounted on a rigid frame that includes a core having an enlarged diameter. The single-use component may further comprise at least one centripetal pump. The single-use structure may be supported within a multi-use rigid drum having an internal truncated cone shape. These configurations allow the exemplary system to maintain a sufficiently high angular velocity to produce a settling velocity suitable for efficiently processing highly concentrated cell culture streams. The feature of minimizing feed turbidity, and other features that allow continuous or semi-continuous discharge of cell concentrate, provides an increase in overall productivity over achievable rates. The exemplary structures and methods provide efficient operation and reduce the risk of contamination.

Drawings

FIG. 1 is a schematic diagram of an exemplary embodiment of a centrifuge system including single-use and multi-use components.

FIG. 2 is a close-up view of the upper flange region of the centrifuge of FIG. 1 illustrating a method of sealing the flexible chamber material to the surface of the flange.

FIG. 3 is an isometric cross-sectional view of the core and upper flange of the single-use component of the embodiment of the centrifuge system of FIG. 1.

FIG. 4 is a schematic view of the embodiment of FIG. 1, wherein the pump chamber of the centrifuge system includes an accelerator vane.

FIG. 5 is an isometric view of the top of the pump chamber of the exemplary embodiment of the centrifuge system shown in FIG. 4.

FIG. 6 is an isometric cross-sectional view of a core, upper flange and lower flange of a single-use centrifuge system with an enlarged core diameter (to create a shallow pool centrifuge) and a feed accelerator.

Fig. 7 is an isometric view of the feed accelerator of fig. 6.

FIG. 8 is an isometric cross-sectional view of a core and upper flange of a single-use centrifuge system having a standard core diameter and a feed accelerator with curved blades and an elliptical bowl.

Fig. 9 is an isometric view of the feed accelerator of fig. 8.

FIG. 10 is a schematic view of a portion of a continuous concentrate discharge centrifuge system.

FIG. 11 is a schematic view of a portion of a second embodiment including a continuous concentrate discharge centrifuge system.

FIG. 12 is a schematic diagram of a continuous concentrate discharge centrifuge system with diluent injection.

FIG. 13 is a schematic view of a portion of a third example embodiment of a continuous concentrate discharge system having a throttle mechanism for a centripetal pump.

FIG. 14 is an isometric cross-sectional view of a core and upper flange of a single-use centrifuge system having a core and a feed accelerator with straight blades.

Fig. 15 is an isometric view of the feed accelerator of fig. 14.

Fig. 16 is an isometric cross-sectional view of an alternative continuous concentrate discharge centrifuge system.

Fig. 17 is an isometric exploded view of an alternative centripetal pump.

FIG. 18 is an isometric view of a plate of an alternative centripetal pump including a volute channel therein.

FIG. 19 is a schematic diagram of a centrifuge system operating to ensure positive pressure is maintained in the centrifuge core chamber.

FIG. 20 is a schematic diagram illustrating a simplified exemplary logic flow executed by at least one control circuit of the system shown in FIG. 19.

Fig. 21 is a schematic cross-sectional view of an alternative continuous centrifuge and concentrate discharge centrifuge system.

FIG. 22 is a schematic cross-sectional view of another alternative continuous centrifuge and concentrate discharge centrifuge system.

FIG. 23 is a schematic cross-sectional view of another alternative continuous centrifuge and concentrate discharge centrifuge system.

FIG. 24 is a schematic diagram of a control system for an exemplary continuous centrifuge and concentrate discharge centrifuge system.

FIG. 25 is a schematic representation of a logic flow associated with the exemplary control system of FIG. 24.

Fig. 26 is a cross-sectional view of an exemplary upper portion of a single-use centrifuge structure including a concentrate dam and a centrate dam located in the separation chamber.

Figure 27 is a cross-sectional view of an exemplary upper portion of a single-use configuration that includes vanes in the centrifuge and concentrate pump chambers to control the radial position of the air/liquid interface.

FIG. 28 is a perspective view of a chamber surface of an exemplary concentrate or centrifuge pump chamber, the chamber surface including a plurality of chamber vanes.

Fig. 29 is a cross-sectional view of an exemplary upper portion of a single-use configuration similar to that shown in fig. 27, showing the location of the air/liquid interface.

Fig. 30 is a cross-sectional view of an exemplary upper portion of a single-use configuration that includes an air channel for retaining pressurized air in an air pocket.

FIG. 31 is a schematic diagram of an exemplary system for controlling a centrifuge system that includes a centrate return pressure control.

Detailed Description

In the field of cell culture for applications in biopharmaceutical processes, there is a need to separate cells from a fluid medium, such as a fluid in which the cells are grown. The desired product from the cell culture may be a molecular species secreted into the medium by the cell, a molecular species retained within the cell, or it may be the cell itself. On a production scale, the initial phase of the cell culture process is typically carried out in a bioreactor, which may be operated in batch mode or continuous mode. Variations, such as repeated batch processes, may also be implemented. The desired product must often be finally separated from other process components prior to final purification and product formulation. Cell harvesting is a general term applied to the separation of these cells from other process components. Clarification is a term referring to cell separation, wherein the target is a cell-free supernatant (or centrate). Cell recovery is a term commonly applied to separations targeting cell concentrates. Exemplary embodiments herein relate to cell harvest separation in large scale cell culture systems.

Methods for cell harvest separation include batch, continuous and semi-continuous centrifugation, Tangential Flow Filtration (TFF) and depth filtration. Historically, centrifuges used to harvest cells of large cell cultures on a production scale are complex multi-use systems that require Cleaning In Place (CIP) and Steam In Place (SIP) technology to provide a sterile environment to prevent contamination by microorganisms. Smaller systems can be used on a laboratory scale and during continuous cell harvesting. The UniFuge centrifuge system manufactured by pnematic Scale Corporation is described in published application US2010/0167388, the entire disclosure of which is incorporated herein by reference, which successfully processes culture batches for cell harvest using batch processing in amounts ranging from 3-30 liters per minute up to about 2000 liters. Also incorporated herein in their entirety are U.S. patent application No. 15/886,382, filed on 1/2/2018; and U.S. patent No. 9,222,067, also owned by pnematic Scale Corporation, the assignee of the present application. Batch processing typically requires periodic stopping of the centrifuge bowl rotation and feed flow in order to discharge the concentrate. This method generally works well in lower concentration, high viability cultures where large batches can be processed and the cell concentrate is discharged relatively quickly and completely.

It is sometimes desirable to harvest cells from a high concentration and/or low viability cell culture that contains a high concentration of cells and cell debris in a material feed, sometimes referred to as a "high turbidity feed". In some centrifugal separation systems, this high turbidity feed slows the process rate because:

1. a slower feed flow rate is required to provide increased residence time in the centrifuge for separation of small cell debris particles, and

2. higher concentrations of cells and cell debris can cause the drum to fill with cell concentrate quickly, requiring the drum to stop to discharge the concentrate.

These combined factors may lead to a reduction in net productivity and unacceptably long cell harvest processing times. In addition to the increased costs that may be associated with longer processing times, the increased time in the centrifuge may also result in higher levels of product contamination and loss when harvesting low viability cell cultures.

High concentrations of cells and cell debris in the material feed may also result in cell concentrates with very high viscosities. This may make it more difficult to completely discharge the cell concentrate from the centrifuge, even under extended discharge cycles. In some cases, additional buffered rinse cycles may be added to obtain a sufficiently complete discharge of the concentrate. The need to make either or both of these adjustments to the drain cycle further increases processing time, which can make the challenge of processing large volumes of cell culture more complex and expensive.

Scaling up the size of the system by increasing the drum size to increase the length of the feed portion of the batch processing cycle is sometimes impractical because it also results in a proportionally longer discharge cycle for the cell concentrate. Another limitation that may prevent simple geometric scaling is the scaling of the relevant hydrodynamic factors. The maximum processing rate of any centrifuge depends on the settling velocity of the separated particles. The settling velocity is given by a modification of the stokes law defined by equation 1:

where v is the sedimentation velocity, Δ ρ is the solid-liquid density difference, d is the particle diameter, r is the radial position of the particle, ω is the angular velocity, μ is the liquid viscosity. With respect to the scaled-up geometry, changing the radius of the drum changes the maximum radial position r that the particles can occupy. Thus, if the other parameters in equation 1 are held constant, an increase in drum radius results in an increase in the average settling velocity and an increase in throughput for a given separation efficiency. However, as the radius increases, maintaining the angular velocity of the drum becomes more difficult due to increased material strength that may be required and other engineering constraints. If the decrease in angular velocity is greater than the square root of the proportional increase in radius, the gains in average settling velocity and throughput (which is proportional to radius) both decrease.

One engineering limitation that must be considered is that the angular velocity required to rotate a larger bowl may not be practical to achieve due to the need for a larger mass and more expensive centrifuge drive platform.

In addition, if the angular velocity remains constant as the radius increases, the force pushing the cells towards the wall of the centrifuge also increases. When the drum is rotated at a sufficiently high angular velocity to produce the desired treatment efficiency, both the walls of the vessel and the cells accumulated therein are subjected to increased stress. For cells, this can cause cell damage by encapsulating the cells to too high a concentration. Cell damage is a disadvantage in applications where cell viability needs to be maintained, and can lead to contamination of the product in solution present in the centrifuge. The higher viscosity resulting from too high a cell concentration is sometimes also a disadvantage of complete discharge of the cell concentrate.

Exemplary embodiments include apparatus and methods for continuous or semi-continuous centrifugation of low viability cell suspension cultures containing high concentrations of cells and cell debris at rates suitable for processing large volumes of cell suspensions on a commercial scale. Some exemplary centrifuges have a pre-sterilized, single-use design and are capable of processing such cell suspensions at flow rates in excess of 20 liters/minute. This flow capacity allows a total run time in the range of 2 to 3 hours for a 2000 liter bioreactor. Exemplary embodiments of single-use centrifuge systems may be capable of processing about 300 to 2000 liters of fluid while operating at a rate of about 2 to 40 liters per minute.

Fig. 1 discloses a single-use centrifuge structure 1000. The centrifuge structure 1000 includes a core structure 1500 (best shown in fig. 3) that includes a core 1510, an upper flange 1300, a lower flange 1200, and a flexible liner 1100 sealed to both the upper flange 1300 and the lower flange 1200. The centrifuge structure 1000 further includes a centrifugal pump 1400 that includes a pair of stationary scraping discs 1410 and a rotating mechanical seal 1700 in a rotating pump chamber 1420.

Centrifuge structure 1000 further includes a feed/discharge assembly 2000. The assembly 2000 includes a plurality of concentric tubes about a rotational axis 1525 (labeled in fig. 12) of the centrifuge 1000. The innermost portion of infeed/outfeed assembly 2000 includes an infeed tube 2100. A plurality of additional tubes concentrically surround the feed tube 2100 and may include a tube or fluid passage 2200 that allows for discharge of centrate, a tube or fluid passage 2500 that allows for discharge of concentrate (see, e.g., fig. 12), or a tube or fluid passage 5000 that allows for supply of diluent (see, e.g., fig. 12). Each portion of the feed/discharge connection may be fluidly connected to a portion of the interior of centrifuge 1000 via an appropriate fluid connection, as well as to a collection or feed chamber (not shown), and may include additional tubes fluidly connected to the concentric tubes to remove centrate, concentrate, or diluent from or add it to the system.

As shown in fig. 1, upper flange 1300 and lower flange 1200 comprise conical drums that are axially aligned with and recessed toward core 1510. Core 1510 comprises a generally cylindrical body having a hollow cylindrical center that is large enough to receive feed tube 2100 having axis 1525 (labeled in fig. 12). The upper flange 1300, core 1510, and lower flange 1200 may be a unitary structure to provide a stronger support structure for the flexible liner 1100, which is also referred to herein as a membrane. In other embodiments, the core structure 1500 may be formed from multiple component parts. In further embodiments, core 1510 and upper flange 1300 may comprise a single component, wherein lower flange 1200 comprises a separate component, or core 1510 and lower flange 1200 may comprise a single component, wherein upper flange 1300 comprises a separate component.

Fig. 3 shows an embodiment of an integral core 1510 and upper flange 1300. This integral component will be joined to the lower flange 1200 to create the internal support structure 1500 of the single-use component of the centrifuge 1000. This structure anchors the flexible liner 1100 at the top and bottom around a fixed internal rigid or semi-rigid support structure 1500. The flexible liner 1100 is also externally supported by the walls and lid of the multi-use structure 3000 when the centrifuge system is in use.

The exemplary separation chamber 1550 is an open chamber that is generally cylindrical in shape, generally bounded by the outer surface 1515 of the core 1510 and the flexible liner 1100, as well as by the upper surface 1210 of the lower flange 1200 and the lower surface 1310 of the upper flange 1300. Separation chamber 1550 is fluidly connected to feed tube 2100 via an aperture 1530 extending from central cavity 1520 of core 1510 to outer surface 1515 of core 1510. The separation chamber 1550 is also fluidly connected to the pump chamber 1420 via a similar aperture 1540 through the core structure 1500. In this example, the bore 1540 slopes upward, toward the pump chamber 1420, opening into the separation chamber 1550 directly below the junction between the core 1510 and the upper flange 1300. As shown in fig. 12,

apertures

1420 or 4420 may enter the pumping chamber at angles other than upward, including horizontally or at a downward angle. Additionally, in some embodiments, the

holes

1420, 4420 may be replaced by slits or gaps between accelerator vanes.

Fig. 1 also shows a feed/discharge assembly 2000 that includes a feed tube carrier 2300 by which a feed tube 2100 extends to the position shown in fig. 3, near the bottom of the centrifuge structure 1000. In this position, feed tube 2100 can perform both feed and discharge functions without movement. By careful design of the gap between the nozzle 2110 of the feed tube 2100 and the upper surface 1210 of the lower flange 1200, the diameter of the nozzle 2110 of the feed tube 2100, and the angular velocity of the centrifuge, shear forces during the feeding process can be minimized. U.S. patent No. 6,616,590, the disclosure of which is incorporated herein by reference in its entirety, describes how to select the appropriate relationship to minimize shear forces. Other suitable feed tube designs known to those skilled in the art that minimize shear forces associated with feeding liquid cell culture into a rotary centrifuge may also be used.

Fig. 1 also includes a centripetal pump 1400 for discharging the centrate through the centrate discharge passage 2200. In the embodiment shown in fig. 1, centrifuge pump 1400 is located above upper flange 1300 in pump chamber 1420. The pump chamber 1420 is a chamber defined by the upper surface 1505 of the core 1510 and the

inner surfaces

1605, 1620 of the centrifuge cover 1600. Centrifuge cover 1600 may include a cylindrical wall 1640 and a mating cover portion 1610 shaped like a generally circular disk (shown in FIG. 5). The centrifuge cover 1600 may be formed as a single piece or from separate components.

As discussed in more detail below, in other embodiments, the shape and location of centrifuge pump chamber 1420 may vary. Chamber 1420 will typically be an axisymmetrical chamber near the upper end of core structure 1500 that is fluidly connected to separation chamber 1550 via an aperture or slit 1530 that extends from near the exterior of core 1515 into centrifuge pump chamber 1420. In some embodiments, as best shown in fig. 11 and 12, the centrifuge pump chamber 1420 may be located in a recess within chamber 1550.

Exemplary centrifuge pump 1400 includes a pair of paring discs 1410. The scraping disk 1410 is two thin disks (plates) that are axially aligned with the axis 1525 of the core structure 1500. In the embodiment shown in fig. 1-5, the scraping discs 1410 are held stationary relative to the centrifuge structure 1000 and are separated from each other by a fixed gap 1415 (labeled 1415 in fig. 10). The gaps 1415 between the scraping discs 1410 form part of a fluid connection for removing centrate from the centrifuge 1000, which allows centrate to flow between the scraping discs 1410 into a hollow cylindrical centrate discharge passage 2200 around the feed tube carrier 2300, which terminates in a centrate outlet 2400.

The exemplary single-use centrifuge structure 1000 is contained within a multiple-use centrifuge structure 3000. Structure 3000 includes a drum 3100 and a cover 3200. The walls of centrifuge bowl 3100 support flexible liner 1100 of centrifuge structure 1000 during rotation of centrifuge 1000. To do so, the exterior structure of the single-use construction 1000 and the interior structure of the multiple-use construction are adapted to each other. Similarly, the upper surface of the upper flange 1200, the exterior of the upper portion of the core 1510, and the lower portion of the wall 1640 of the centrifuge cover 1600 conform to the inner surface of the multi-use drum cover 3200, which is also adapted to provide support during rotation. Features of the multi-use bowl 3100 and bowl cover 3200 (discussed in more detail below) are designed to ensure that shear forces do not tear the liner 1100 from the single-use centrifuge structure 1000. In some cases, an existing multi-use structure 3000 can be retrofitted for single-use disposal by selecting a conforming single-use structure 1000. In other cases, the multiple-use configuration 3000 may be specifically designed for use with a single-use configuration insert 1000.

Fig. 2 illustrates a portion of an exemplary structure for an upper flange 1300, a plastic liner 1100, and a lid 3200 of a multiple use centrifuge structure 3000 to illustrate the sealing of the flexible liner 1100 to the upper flange 1300. The flexible liner 1100 may be a thermoplastic elastomer such as polyurethane (TPU) or other stretchable, tough, non-tearing, biocompatible polymer, while the upper and

lower flanges

1300, 1200 may be made of a rigid polymer such as polyetherimide, polycarbonate, or polysulfone. The flexible liner 1100 is a thin sleeve or envelope that extends between and seals to the upper and

lower flanges

1300, 1200 and forms the outer wall of the separation chamber 1550. The composition of the pad 1100 and the upper and

lower flanges

1300, 1200 and the core 1510 described herein are exemplary only. Those skilled in the art may substitute suitable materials having properties similar to those suggested that are known or that may become known.

A thermal bond attachment process may be used to bond the different materials in the areas shown in fig. 2. The thermal bond 1110 is formed by preheating the flange material, placing the elastomeric polymer on top of the heated flange, and applying heat and pressure to the elastic film liner 1100 at a temperature above the softening point of the film. The plastic liner 1100 is bonded to the lower flange 1200 in the same manner. Although thermal bond 1110 is described herein, it is merely exemplary. Other means of creating a similarly strong relatively permanent bond between the flexible membrane and the flange material may be substituted, such as by temperature, chemical, adhesive or other bonding means.

Exemplary single-use components are pre-sterilized. During removal of the components from their protective packaging and installation into a centrifuge, the thermal bonds 1110 remain sterile within the single-use chamber. When in use, the stretchable flexible liner 1100 conforms to the walls of the reusable bowl 3100. The reusable bowl 3100 provides sufficient support and the flexible liner 1100 is sufficiently resilient to allow the single use configuration 1000 to withstand the increased rotational forces that arise when a larger radius centrifuge 1000 is filled with liquid cell culture or other cell suspension and rotated at sufficient angular velocity to reach a settling velocity that allows processing at a rate of about 2 to 40 liters per minute.

In addition to thermal bonds 1110, sealing ridges or "nubs" 3210 may be present on the drum cover 3200 to press the thermoplastic elastomer film against the rigid upper flange 1300 to form additional seals. The same compression seal may also be used at the bottom of the bowl 3100 to seal the thermoplastic elastomer film to the rigid lower flange 1200. These compression seals support thermal bond area 1110 by isolating it from shear forces created by hydrostatic pressure generated when the chamber is filled with liquid during centrifugation. The combination of thermal bond 1110 and compression nub 3210 seal has been tested at 3000 times gravity (3000x g), which corresponds to a hydrostatic pressure of 97psi at the drum wall. The pad should be thick and compressible enough to allow nubs 3210 to compress and grip flexible pad 1100 while minimizing the risk of tearing near thermal bond 1110 or compression nubs 3210. In one example embodiment, a 0.010 inch thick flexible TPU gasket is sealed without tearing or leaking.

Embodiments corresponding to the illustrations of fig. 1-2 were tested in a 5.5 inch diameter drum. It has a hydraulic capacity of > 7 liters per minute at 2000 times gravity and successfully separates mammalian cells to 99% efficiency at a rate of 3 liters per minute.

In most cases, the upper and

lower flanges

1300, 1200 can have a shape similar to that shown in fig. 1, but in some cases the upper surface of the single use centrifuge structure can have a different shape, as shown in fig. 10 and 11. In the embodiment shown in fig. 10 and 11, rather than having a generally conical drum cover 3200 to accommodate the generally conical upper flange 1300, both the upper flange and the drum cover are relatively disc-shaped. Those skilled in the art will be able to adapt the sealing techniques described herein to sealing surfaces of different shapes.

Fig. 4-5 illustrate example embodiments having features that improve the efficiency of a centripetal pump 1400. As shown in detail in fig. 5, this embodiment for the internal structure of a single-use component similar to that shown in fig. 1 and 2 includes a plurality of radial fins 1630 on the inner surface 1620 of the cap 1610 of the pump chamber 1420. Fig. 5 shows the inner surface 1620 of the cap 1610 of the centrifuge cover 1600. The radial tabs 1630 may be thin, generally rectangular radial plates that extend perpendicularly from the inner surface 1620 of the cap 1610. In this exemplary embodiment, six (6) fins 1630 are shown, but other embodiments may include fewer or more fins 1630. In this embodiment, the tab 1630 forms a portion of the inner surface of the cap 1620, but in other embodiments may comprise an upper surface 1620 of the pump chamber 1420, which may take a different form than the cap 1610. When the centrifuge system 1000 is in use, the vanes 1630 are positioned above the scraping disk 1410 of the centripetal pump 1400 in the chamber 1420. These vanes 1630 transmit the angular rotation of the centrifuge 1000 to the centrifugal contents of the pump chamber 1420.

This increases the efficiency of the centripetal pump 1400, stabilizes the gas-liquid interface in the pump chamber 1420 above the scraping disk 1410, and increases the size of the gas barrier. The gas barrier is a generally cylindrical column of gas that extends outward from the exterior of the infeed/outfeed mechanism 2000 into the pump chamber 1420 to the inner surface of the spinning centrifuge. This increase in barrier size also occurs because the resulting increase in angular velocity of the centrifuge forces the centrifuge toward the centrifuge wall. When the rotating centrifugal matter contacts the stationary scraping disk 1410 within the pump chamber 1420, the resulting friction may reduce the efficiency of the pump 1400. A plurality of radial vanes 1630 are added, which rotate at the same angular velocity as the centrifugal mass, overcoming any reduction in velocity that might otherwise result from the collision between the rotating centrifugal mass and the stationary scraping disk 1410.

FIG. 6 illustrates an exemplary embodiment of an improved core structure 1500 for high turbidity feeds. Core structure 1500 includes a core 1510, an upper flange 1300, and a lower flange 1200. Core 1510 has a cylindrical central cavity 1520 adapted to allow insertion of feed tube 2100 into central cavity 1520. The distance from the central axis 1525 to the exterior of the core 1515 (core width, indicated by dashed line 6000 in fig. 6) is greater than the corresponding distance in the embodiment shown in fig. 3. The larger diameter core 1510 reduces the depth of separation chamber 1550 (represented by dashed line 6010) so that centrifuge 1000 operates as a shallow pool centrifuge. The depth 6010 of the separation chamber 1550 is generally the distance between the outside of the core 1510 and the flexible liner 1100, as marked in fig. 1 and 12. A shallow pool centrifuge is a centrifuge with a depth 6010 that is small relative to the diameter of the centrifuge. As can be seen in the exemplary embodiment shown in fig. 12, to facilitate removal of cell concentrate, shallow pool depth 6010 may vary from shallower at the bottom of separation chamber 1550 to slightly deeper at the top of separation chamber 1550. In some embodiments shown herein, the ratio of the average separation cell depth 6010 to the core width is 1:1 or less. One example of a shallow pool centrifuge is provided as an alternative model to the ViaFuge centrifuge system manufactured by pnematic Scale Corporation. An advantage of a shallow pool centrifuge is that it enables separation at higher feed flow rates. This is achieved by a higher average gravity for a given inner drum diameter, which results in a higher settling velocity at a given angular velocity. The resulting enhanced separation performance is beneficial when separating highly turbid feeds containing high concentrations of cell debris.

The example embodiment of the core structure 1500 shown in fig. 6 also includes accelerator blades 1560 as part of the lower flange 1200. The accelerator blade 1560 (shown in fig. 12) (rather than an aperture 1530 through the solid core 1510 (shown in fig. 10-11)) includes an alternative embodiment of a fluid connection between the central cavity 1520 of the core 1510 and the separation chamber 1550.

In the exemplary embodiment of the core structure 1500 shown in fig. 6, the accelerator blades 1560 comprise a plurality of radial, generally rectangular, spaced-apart webs 1580 that extend upward from the upper conical surface of the lower flange 1200. The plates 1580 extend upward perpendicular to the base of the core 1510. The plates 1580 extend generally radially outward from about the axis 1525 of the core 1510. In an exemplary embodiment, there are 12 plates 1580, as best shown in fig. 7. In other embodiments, there may be fewer or more than 12 plates 1580. Additionally, in other embodiments, plate 1580 may be curved in the direction of rotation of centrifuge 1000, as shown in the exemplary embodiment in fig. 9. The inner surface of the lower flange 1200 may be modified to form an oval accelerator drum 1590 with a curved plate extending upwardly therefrom. These embodiments are intended to be exemplary and one skilled in the art may combine them in different ways or may modify these embodiments to further benefit from the turbidity reduction of the plates and the shape produced by the lower flange 1200 and/or the embedded accelerator drum.

Other features of an exemplary embodiment of a single-use centrifuge 1000 designed for continuous or semi-continuous operation are shown in fig. 10-12. The exemplary embodiment shown in fig. 10 includes a second centripetal pump 4400 for removing cell concentrate. The centripetal pump 4400 for removing cell concentrate is positioned above the centripetal pump 1400 for removing centrifugation. The radial pump 4400 includes a pump chamber 4420 and a scraping disk 4410. A plurality of apertures or continuous slits 4540 extend from the upper outer circumference of the separation chamber 1550 into the pumping chamber 4420 providing a fluid connection from the exterior of the separation chamber 1550 to the second pumping chamber 4420. As with pump chamber 1400, pump chamber 4400 may have a different shape than that shown in fig. 10-12, but will be generally an axially symmetric chamber near the upper end of core structure 1500 that is fluidly connected to separation chamber 1550. As with pump chamber 1400, the pump chamber may be partially or fully recessed within core structure 1500. If the centrifuge pump chamber 1400 is present near the upper end of the core structure 1500, the cell concentrate pump chamber 4400 will be located generally above it. The pump chamber 4400 for removing cell concentrate will be fluidly connected to the separation chamber 1550 via an aperture or slit 4540 extending from near the outer upper wall of the separation chamber 1550 for collecting heavier cell concentrate propelled thereto by centrifugal force.

In the embodiment shown in fig. 10, the radius of the scraping disk 4410 for the concentrate discharge pump 4400 is substantially the same as the radius of the scraping disk for the centrifuge discharge pump 1400 and is rotationally fixed. In other embodiments, such as the embodiment shown in fig. 11, the scraper discs 4410 in the concentrate discharge pump 4400 may have a larger radius than in the centrifuge discharge pump 1400, with a correspondingly larger pump chamber 4420. Various intermediate diameter scraping discs may also be used. The optimum diameter will depend on the nature of the cell concentrate to be discharged. Larger diameter paring discs have higher pumping capacity but produce more shear.

In the embodiment shown in fig. 1, 4 and 10, the scraping disk 4410 in the concentrate discharge pump 4400 is rotationally fixed. In other embodiments, such as the embodiment shown in fig. 11, the scraping disk 4410 may be adapted to rotate at an angular velocity between zero and the angular velocity of the centrifuge 1000. The desired angular velocity may be controlled by a variety of mechanisms known to those skilled in the art. One example of a control device is an external slip clutch that allows the scraping disk 4410 to rotate at an angular velocity that is a fraction of the angular velocity of the centrifuge 1000. Other means of controlling the angular velocity of the scraping disc will be apparent to the skilled person.

In the embodiment shown in fig. 1, 4, 10 to 12, the

gap

1415, 4415 between the scraping

discs

1410 and 4410 is fixed. In other embodiments, such as the embodiment in fig. 13, the

gaps

1415, 4415 between the scraping

discs

1410 and 4410 may be adjustable to control the flow rate at which the centrate or concentrate is removed from the centrifuge 1000. One of each pair of scraping

discs

1410 and 4410 is attached to a vertically movable throttle 6100. The throttle 6100 can be moved up or down to narrow or widen the

gap

1415, 4415 between each pair of scraping

discs

1410, 4410. Additionally, an external peristaltic pump 2510 (not shown) may be added to the concentrate removal line 2500 (not shown) to assist in removing the concentrate. This pump 2510 can be controlled by a sensor 4430 in a pump chamber 4420. A sensor 4430 (not shown) may also be used to control the diluent pump 5150 to synchronize the removal of concentrate with the addition of diluent.

Also shown in fig. 13 is an embodiment in which a centrifuge pump 1400 is located at the base of the centrifuge 1000. In the embodiment shown in fig. 13, a centrifuge well 1555 is created between the pumping chamber 1420 and the flexible liner 1100. An aperture 1530 extends from core 1510 into centrifuge well 1555 below pump chamber 1420. Additionally, in the exemplary embodiment shown, a bore 1540 extends from separation chamber 1550 into pump chamber 1420 adjacent to outer surface 1515 of core 1510 to allow removal of centrate using centrate pump 1400. A bore 4540 may also extend from between separation chambers 1550 to pumping chamber 4420 adjacent the upper surface of the exterior of the separation chamber to allow cell concentrate to flow into pumping chamber 4420 for removal using radial pump 4400.

As described above, in the exemplary embodiment shown, the

clearances

1415, 4415 between the scraping

discs

4410 and 1410 can be adjusted by using a throttle pipe 6100 connected to one of each pair of scraping

discs

4410, 1410. The throttle pipe 6100 and the attached one of each

scraper disk pair

4410, 1410 may move up or down to narrow or widen the

gap

1415, 4415. In the exemplary embodiment shown, the throttle pipes 6100 are attached to the lower and upper paring discs of the paring

disc pair

4410, 1410, respectively. In other embodiments, the attachment may be reversed, may be used to throttle a single centripetal pump, or may be used to throttle both in parallel (rather than the opposite as shown in fig. 13).

As can be seen in the embodiment shown in fig. 10-12, the walls of the solid multi-use bowl 3100 are thicker at the base than in the upper portion thereof so as to create an internal truncated cone shape to support the single-use centrifuge structure 1000, which has a smaller radius at the lower end than at the upper end. This larger radius at the upper end of separation chamber 1550 moves the denser cell concentrate toward the upper exterior of separation chamber 1550 and into centripetal chamber 4420. In the embodiment shown, this truncated cone shape is produced by a multiple use drum 3100 having thicker walls at the base than it is in the upper part. Those skilled in the art will recognize that the multi-use drum 3100 having an internal truncated cone shape may also include walls of uniform thickness, and that other variations may exist that produce the desired internal shape of the multi-use drum 3100.

In the exemplary embodiment shown in fig. 10-12, the feed mechanism 2000 further comprises additional passages for removing cells or cell concentrates. In the embodiment shown in fig. 1, a cylindrical passageway 2200 around the feed tube 2100 is used to remove the centrate. The embodiment shown in fig. 10-12 also includes a concentric cylindrical passageway, referred to as a cell drain 2500, for removing cells or cell concentrate. Cell drain 2500 surrounds centrate removal passage 2200. If the centrifuge is designed to be used with concentrates that are expected to be very viscous, additional concentric cylindrical fluid passageways 5000 may be added around the feed tube 2100 to allow for the introduction of diluent into the cell concentrate pump chamber 4420 in order to reduce the viscosity of the concentrate. In the exemplary embodiment shown in fig. 12, the diluent passage 5000 comprises a concentric tube surrounding the cell discharge passage and opens at the lower end to a thin disk-shaped fluid passage 5100 above the scraping disk 4410, discharging near the outer edge of the scraping disk 4410 to provide fluid communication with the pump chamber 4420. Injecting the diluent at this location in this manner limits the diluent from mixing with the concentrate and being discharged with the concentrate rather than being introduced into the centrifuge, which may be undesirable in some applications. In alternative embodiments, the dilution liquid may be introduced directly onto the upper surface of the paring disc and allowed to expand radially outwards, or onto a separate disc located above the paring disc.

The choice of diluent will depend on the purpose of the separation process and the nature of the cell concentrate to be diluted. In some cases, a simple isotonic buffer or deionized water may be used as the diluent. In other cases, a diluent specific to the nature of the cell concentrate may be advantageous. For example, in production scale batch cell cultures operated at low cell viability, a flocculant is typically added to the culture as it is fed to the centrifuge to cause cells and cell debris to flocculate or aggregate into larger particles, which facilitates their separation by increasing their settling rate. Since both cells and cell debris carry a negative surface charge, compounds used as flocculants are typically cationic polymers, which carry multiple positive charges, such as polyethyleneimine. Due to its multiple positive charges, this flocculant can link negatively charged cells and cell debris into large aggregates. An undesirable consequence of using such a flocculant is that it further increases the viscosity of the cell concentrate. Thus, a diluent that is particularly useful in the present application is a deflocculant that will disrupt the binding that increases the viscosity of the cell concentrate. Examples of deflocculants include high salt buffers, such as sodium chloride solutions, at concentrations of 0.1M to 1.0M. Other deflocculants that can be used to reduce the viscosity of the cell concentrate are anionic polymers, such as polymers of acrylic acid.

In the case of cell concentrates in which cell viability is to be maintained, a diluent may be selected which is a shear protectant, such as dextran or Pluronic F-68. The use of a shear protectant, in combination with an isotonic buffer, will enhance the survival and viability of the cells upon discharge from the centrifuge.

The exemplary centrifuge shown in fig. 4 operates as follows. During a feed cycle, a feed suspension flows through feed tube 2100 into a rotating drum assembly. As the feed suspension enters the central cavity 1520 of the core 1510 adjacent the lower flange 1200, it is pushed outward by centrifugal force along the upper surface of the lower flange 1200, through the holes 1530 in the core 1510 and into the separation chamber 1550.

The centrate collects in the separation chamber 1550, i.e., a hollow generally cylindrical space around the core 1510 below the upper flange 1300. The centrate flows upwardly from its inlet through the bore 1530 into the separation chamber until it encounters the bore 1540 located between the separation chamber 1550 and the pump chamber 1420 adjacent the core 1410 in the upper part of the separation chamber 1550. Particles having a density higher than the liquid density move by settling (particle concentrate) towards the outer wall of the separation chamber 1550, away from the aperture 1530. When the rotation of centrifuge 1000 stops, the particulate concentrate moves downward under the influence of gravity to nozzle 2110 of feed tube 2100 for removal via combined feed/discharge mechanism 2000.

During rotation, centrifuge enters centrifuge pump chamber 1420 through aperture 1540. In the pump chamber 1420, the rotating centrifugal mass encounters the stationary scraping disk 1410, which converts the kinetic energy of the rotating liquid into pressure that pushes the upwardly discharged centrifugal mass through the centrifugal mass discharge passage 2200 in the feed/discharge mechanism 2000 and discharges it through the centrifugal mass discharge pipe 2400.

By adding radial fins 1630 on the inner surface 1620 of the cap 1610 of the rotary pump 1400, the efficiency of the centrifugal pump 1400 is increased. These vanes 1630 transfer the angular momentum of the rotating assembly to the centrifugal mass in the pump chamber 1420, which may otherwise slow due to friction as the rotating centrifugal mass encounters the stationary scraping disk 1410. The centrifugal pump 1400 provides an improved way of centrifugal discharge over mechanical seals due to the gas-liquid interface within the pump chamber 1420. The gas within the pump chamber 1420 is isolated from contamination of the external environment by the rotating seal 1700. Since the centrate discharged between the scraping discs 1410 is not in contact with air during either the feed or discharge process, it avoids excessive foaming that often occurs when the discharge process introduces air into the cell culture.

In the embodiment of centrifuge 1000 shown in fig. 4-5, the cell concentrate is discharged by periodically stopping the bowl rotation and feed flow and then pumping out the cell concentrate that has collected along the outer wall of separation chamber 1550. This process is called batch processing. When the volumetric capacity of the separation chamber 1550 is reached, centrifugal rotation is stopped. The cell concentrate moves down towards the nozzle 2110 of the feed tube 2100, where it is withdrawn by pumping the concentrate through the feed tube 2100. Suitable valves (not shown) external to centrifuge 1000 are used to direct the concentrate into a collection vessel (not shown). If the entire bioreactor batch has not been completely processed, drum rotation and feed flow is resumed, followed by additional feed and discharge cycles until the entire batch is processed.

As described above, when the cell culture is concentrated or contains a large amount of cell debris, the above process slows down because the residence time must be increased to capture small debris particles, which necessitates slower feed flow rates and fast filling of the separation chamber 1550, and frequent and repeated stops of rotation for each culture batch. In addition, cell concentrates tend to be more viscous, so gravity cannot effectively drain the cell concentrate to the bottom of centrifuge 1000, taking longer, and in some cases, washing may be required to remove the remaining cells.

As improved in the exemplary embodiments shown in fig. 6-13, a single use centrifuge produces a higher average sedimentation velocity without increasing angular velocity, allows the centrifuge 1000 to run continuously or semi-continuously, and allows diluent to be added to the cell concentrate during the cell removal process, making removal of cells easier and more complete.

The embodiment of the single-use centrifuge structure 1000 shown in fig. 6-12 operates as described herein. The feed suspension enters single-use centrifuge configuration 1000 via feed tube 2100. As the feed suspension encounters accelerator blade 1560, blade 1560 imparts an angular velocity to the feed suspension that approximates the angular velocity of single-use centrifuge 1000. The use of vanes 1560 instead of apertures 1530 provides a greater volume of feed suspension to enter separation chamber 1550 through a slower radial velocity, avoiding jetting that occurs when the feed suspension is forced through apertures 1530 having smaller cross-sectional openings than the openings between vanes 1560. This reduction in feed stream velocity minimizes disruption of the liquid contents in the tank as the feed stream enters the separation zone or tank, which allows for more efficient settling.

As centrifuge 1000 rotates, particles having a density greater than the centrate are pushed outward of separation chamber 1550, leaving a particle-free centrate adjacent core 1510. Centrifuge bowl 3100 has the shape of an inverted truncated cone with a wider radius at the upper end than at the lower end. Centrifugal forces cause particles to collect in the upper and outer parts of the chamber. Centrifuge 1000 may operate in a semi-continuous discharge of concentrate. The centrate discharge generally operates as described with reference to figure 4. Cell concentrate discharge works similarly, where cell concentrate collects near the upper exterior of the separation chamber 1550 and enters the concentrate discharge pump chamber 4400 via the aperture 4540 near the upper exterior wall of the separation chamber 1550.

A vibration sensor system, such as that described in U.S. patent No. 9,427,748, which is incorporated herein by reference in its entirety, may be used to monitor the feed rate and rotational angular velocity of the suspension. Such a sensor system allows the centrifuge to be filled at a lower rate until the vibration indicates that the centrifuge is nearly full, and then the feed rate and angular velocity are appropriately adjusted in response to this information. Typically, once the centrifuge is close to full, the feed rate is reduced or stopped, and the angular velocity will increase to increase the settling velocity, and once settling and discharge are substantially complete, the cycle will repeat. If the system is optimized using the additional features described herein to reduce the need to interrupt the process, it is possible to operate the system continuously or nearly continuously at the angular velocity required for settling.

In the case of semi-continuous concentrate discharge, the suspension is continuously fed into the centrifuge 1000 using a concentrate pump 4400 that operates intermittently to remove the concentrate. The operation of the concentrate pump 4400 may be controlled by an optical sensor in the concentrate discharge line that indicates the presence or absence of discharged concentrate. Instead of the concentrate pump 4400, the discharge cycle may be electronically managed using a controller and sensors that determine when to open and close the valve to most effectively process the fluid suspension.

The average discharge rate can be further controlled by using a centrifuge 1000 with an adjustable gap between the paring

discs

4410, 1410. It should be noted that it may only be desirable or necessary that one set of scraping

discs

4410, 1410 be adjustable. The gap between the scraping discs 4410, 1410 (which forms part of the fluid path exiting the centrifuge 1000) may be opened to allow flow or closed to shut off flow, thereby acting as an internal valve. Widening or narrowing the

gap

4415, 1415 between the scraping

discs

4410, 1410 may also be useful depending on the desired product or characteristics of the product. Changing the clearance affects the pumping and shear rates associated with the scraping disk.

The rate at which concentrate and centrate are removed from the centrifuge 1000, as well as the viability of the removed concentrate, may be further controlled using a number of features of the exemplary embodiments shown in fig. 4-13. Accelerator vanes 4630, similar to those in the centrifuge pump chamber 1420, may be added to the concentrate pump chamber 4420. The addition of the accelerator vanes 4630 increases the rate at which concentrate can be removed by overcoming some of the deceleration due to friction between the moving concentrate and the scraping disk 4410. In addition to accelerator tabs 4630 in the upper surface of pump chamber 4420, such tabs 4630 may also be added to the lower surface in pump chamber 4420 to increase its effectiveness. Another feature may be to replace the

apertures

1540, 4540 with slits, which minimizes shearing on the material entering the

pump chambers

1420, 4420.

If the vigor of the concentrate is important, a rotatable scraping disc 4410 may be included in the pump chamber 4420, which reduces the shear force applied to the concentrate when the concentrate contacts the surface of the scraping disc 4410. The rate of rotation of the scraping disk 4410 can be adjusted to a rate somewhat between the fixed value and the rate of rotation of the separation chamber 1550 to balance the concentrate vigor and the discharge rate. The desired angular velocity may be controlled by a variety of mechanisms known to those skilled in the art. One example of a control device is an external slip clutch that allows the scraping disk to rotate at an angular velocity that is a fraction of the angular velocity of the centrifuge. The use of slip clutches is well known to those skilled in the art. In addition, there may be devices other than slip clutches to adjust the angular velocity, as will be apparent to those skilled in the art.

Peristaltic pumps 2510 may also be used to make the removal of the concentrate more efficient and reliable, especially for very concentrated feed suspensions. The use of the peristaltic pump 2510 allows a user to more accurately control the flow rate of concentrate from the centrifuge 1000 than is possible by relying on the centrifugal pump 4400 alone, as the rate of the centrifugal pump is not as easily adjusted as the rate of the peristaltic pump 2510.

In addition, to reduce the viscosity of the concentrate, a diluent pump 5150 may be used to pump a diluent such as sterile water or buffer through the diluent passage 5000 into the concentrate pump chamber 4420. A more complete useful discussion of the diluent can be found above. The operating rate of either or both of the peristaltic pump 2510 or the diluent pump 5150 may be controlled by an automatic controller (not shown) responsive to a concentration sensor 4430 located in the concentrate discharge connection 2500. The controller can be programmed to start, stop, or vary the pumping rates for diluent addition and concentrate removal in response to the concentration of particles in the concentrate (independently in response to the concentration sensor 4430, in conjunction with a standard feed/discharge cycle, or as a combination).

FIG. 16 illustrates an alternative example embodiment of a core for use in conjunction with a centrifuge that provides a continuous separation process to produce a continuous concentrate and centrate supply. The core 10 is similar to those previously discussed, and is configured to be positioned in a rotatable bowl of a centrifuge. During processing, the centrifuge bowl and core rotate about axis 12. The apparatus includes a stationary assembly 14 and a rotatable assembly 16.

As with the previously described embodiment, the stationary assembly 14 includes a feed tube 18. The feed tube 18 is coaxial with the axis 12 and terminates in an opening 20 at the bottom of a separate chamber or cavity 22 adjacent the core. The stationary assembly further includes a centrifuge centripetal pump 24, and an exemplary embodiment of the centrifuge pump 24, described in more detail below, includes an inlet 26 and an annular outlet 28. The annular outlet is fluidly connected to a centrifuge tube 30. The centrifuge tube extends in coaxial surrounding relation to feed tube 18.

In this exemplary embodiment, the centrifuge centripetal pump 24 is positioned within the centrifuge pump chamber 32. The centrifuge pump chamber is defined by a wall that is part of the rotatable assembly and which provides the inlet 26 of the centrifuge centripetal pump during operation for exposure to a pool of liquid centrifuge.

The exemplary embodiment also includes a concentrate centripetal pump 34. The concentrate centripetal pump 34 of this exemplary embodiment may also have a configuration similar to that discussed in detail later. In the exemplary arrangement, the concentrate centripetal pump 34 includes an inlet 36 positioned in a wall defining an annular periphery of the centripetal pump. It should be noted that the concentrate centripetal pump 34 has a peripheral diameter that is greater than the peripheral diameter of the centrifugal pump. The concentrate pump also includes an annular outlet 38. The annular outlet 38 is fluidly connected to a concentrate outlet tube 40. The concentrate outlet tube extends in coaxial surrounding relationship with the centrifuge tube 30.

In the exemplary embodiment, the concentrate centripetal pump inlet 36 is positioned in a concentrate pump chamber 42. The concentrate pump chamber is defined by the walls of the rotatable assembly 16. During operation, the concentrate is exposed to the inlet 36 of the centrifugal pump to concentrate in the concentrate pump chamber 42. The concentrate pump chamber 42 is vertically bounded by a top 44. At least one fluid seal 46 extends between the outer circumference of the outlet tube 40 and the top 44. The example seal 46 is configured to reduce the risk of fluid escaping from the interior of the separation chamber and prevent contaminants from being introduced from the exterior region of the core therein.

During operation of the centrifuge, the bowl and the core including the cavity or separation chamber rotate in a rotational direction about axis 12. Rotation in the direction of rotation is operable to separate a cell suspension introduced through the feed tube 18 into a centrate discharged through the centrate tube 30 and a concentrate discharged through the concentrate outlet tube 40.

The cell suspension enters the separation chamber 22 through the tube opening 20 at the bottom of the separation chamber. The cell suspension moves outward via centrifugal force and a plurality of accelerator blades 48. As the suspension moves outwardly through the accelerator blades, centrifugal forces act on the cell suspension material causing the cell material to move outwardly towards the annular tapered wall 50 defining the outside of the separation chamber. As shown, the concentrated cellular material is urged to move outwardly and upwardly against the tapered wall 50 and through a plurality of concentrate troughs 52. The concentrate material moves upwardly beyond the concentrate trough and into the concentrate pump chamber 42 from which the concentrate is discharged by the concentrate centrifugal pump 34.

In the exemplary arrangement, during operation, the cell-free centrifuge is positioned proximate to the vertical annular wall 54 that defines the interior of the separation chamber 22. The centrifuge material moves upwardly through a centrifuge aperture 56 in an annular base structure defining the centrifuge pump chamber 32. The centrate moves upward through the centrate aperture 56 and forms a pool of liquid centrate in the centrate chamber. The centrate is moved from the centrate chamber by operation of the centrate centrifugal pump 24 and is delivered from the core through the centrate tube 30.

In the exemplary embodiment of fig. 16, the concentrate pump and the centrifuge pump may have a configuration substantially similar to that shown in fig. 17. In fig. 17, the centrifuge centripetal pump 24 is shown in an isometric exploded view. As shown in fig. 17, the exemplary centripetal pump has a disc-shaped body formed by a first plate 58 and a second plate 60. During operation, the first and second plates are held in releasably engaged relationship via fasteners represented by screws 62. It should of course be understood that in other embodiments, other configurations and fastening methods may be used.

In this exemplary arrangement, the second plate 60 includes three side walls that define a curved volute channel 64. It should be understood that while in this exemplary arrangement, the centripetal pump includes a pair of generally opposed volute channels 64. In other arrangements, other numbers and configurations of volute channels may be used.

In this exemplary arrangement, the first and second plates constitute a disc-shaped body of the centripetal pump having an annular vertically extending wall 67 defining an annular periphery 66. An inlet 68 to the volute passage 64 extends in the annular periphery. An annular collection chamber 70 extends radially outward from the axis 12 in the disc-shaped body and is fluidly connected to the volute passage. An annular collection chamber 70 receives material entering the inlet 68. The annular collection chamber 70 is fluidly connected to an annular outlet coaxial with the axis 12. In an exemplary arrangement of the centrifuge centripetal pump, the annular outlet is an annular space extending between the outer wall of the feed tube 18 and the inner wall of the second plate 60, which outlet is fluidly connected to the centrifuge outlet tube 30.

In this exemplary arrangement, each volute channel 64 is configured such that the volute channel curves toward the direction of rotation of the bowl and separation chamber, which is indicated by arrow R in fig. 17. In this example structure, the vertically extending walls 74 that define the volute channels and face the direction of rotation are each curved toward the direction of rotation. The curved configuration of the wall 74 horizontally bounding the volute passage provides enhanced pumping characteristics of this exemplary arrangement. Furthermore, the opposing bounding walls 76 of each volute channel in this exemplary arrangement have a similar curved configuration. The curved configuration of the vertically extending wall horizontally bounding the volute channels provides a constant cross-sectional area of each volute channel from the respective inlet to the collection chamber. This uniform cross-sectional area is further achieved by using a generally flat wall 78 that extends between

walls

74 and 76 and vertically defines a volute channel on one side. Furthermore, in the exemplary embodiment, first plate 58 includes a generally flat circular face 80 on a side thereof that faces inwardly when the plates are assembled to form a disc-shaped body of a centripetal pump. In this exemplary arrangement, the face 80 serves to vertically bound the sides of the two volute channels 64 of the heart pump.

Of course, it will be understood that this exemplary arrangement including a pair of plates is exemplary, with one plate including a recess having a wall defining three of the four sides of the curved volute passage and the other plate including a surface defining the remaining side of the volute passage. It should be understood that in other arrangements, other configurations and structures may be used.

In the exemplary centripetal pump structure shown in fig. 16, a centripetal pump structure is used and has the ability to move more liquid than a scraped disc type centripetal pump of the same size. Furthermore, this exemplary configuration produces less heating of the liquid than a comparable paring disc.

Furthermore, in the exemplary arrangement as previously described, the annular periphery of the centrate centripetal pump 24 has a smaller outer diameter than the periphery of the concentrate centripetal pump 34. In this exemplary arrangement, this configuration serves to avoid the centrifuge centripetal pump removing excess liquid from the pool of liquid centrate formed in the centrifuge pump chamber 32. Ensuring that there is sufficient liquid centrate within the centrate pump chamber helps ensure that no waves are formed in the centrate near the inlet of the centrate centrifugal pump. Waves formed due to insufficient liquid centrate may cause vibration and other undesirable properties of the centrifuge and core.

The larger annular perimeter of the concentrate pump of this example arrangement results in material preferentially flowing out of the core via the concentrate centripetal pump. In this exemplary arrangement, the concentrate flow downstream of the concentrate outlet conduit 40 can be controlled to control the ratio of the centrate flow to the concentrate flow from the core.

Further, in exemplary embodiments, with a centripetal pump having the described configuration, the properties and flow characteristics of the centrifuge may be tailored to the requirements of the particular material and separation process being performed. In particular, the diameter of the annular periphery of the centripetal pump may be sized to achieve optimal properties for a particular treatment activity. For example, the larger the diameter of the periphery of the centripetal pump, the greater the flow and pressure achievable at the outlet. In addition, larger diameters tend to produce greater mixing than relatively smaller diameters. However, the larger diameter also results in greater heating than the smaller peripheral diameter of the centripetal pump. Thus, to achieve less heating, a smaller diameter perimeter may be used. Further, it should be understood that different sizes, areas and numbers of inlets, as well as different volute channel configurations, may be utilized as desired to vary flow and pressure properties for the purposes of a particular separation process.

Fig. 19 schematically illustrates an exemplary system for helping to ensure positive pressure within a separation chamber, alternatively referred to herein as a chamber, during cell suspension processing. As discussed in connection with the previous exemplary embodiments, it is generally desirable to ensure a positive pressure above atmospheric pressure within the separation chamber at all times. Doing so reduces the risk of contaminants being introduced into the separation chamber by permeating through one or more fluid seals operatively extending between the fixed and rotatable components of the core. As further noted above, it is also generally desirable to maintain air at positive pressure within the separation chamber in contact with the inner surface of the fluid seal. The presence of an air pocket adjacent the seal avoids the seal coming into contact with the material being processed and further helps to reduce the risk of contaminants being introduced into the processed material and any material escaping from the separation chamber.

The exemplary system described in connection with fig. 19 is used to maintain a consistent positive pressure in the separation chamber and reduce the risk of the introduction of contaminants and the escape of processed materials.

As schematically shown in fig. 19, the centrifuge includes a rotatable bowl 82. The centrifuge bowl may be rotated about axis 84 by a motor 86 or other suitable rotating device.

The exemplary centrifuge structure shown includes a rotatable single-use core 88 that defines a cavity 90, which is alternatively referred to herein as a separation chamber.

Similar to other previously described embodiments, the exemplary core includes a stationary assembly including a suspension inlet feed tube 92 having an inlet 94 located near a bottom region of the chamber. The stationary assembly further includes at least one centripetal pump 96. The centripetal pump of the exemplary embodiment includes a disc-shaped body having at least one pump inlet 98 adjacent its periphery and a pump outlet 100 adjacent the center of the centripetal pump. The pump outlet is fluidly connected to the centrate outlet tube 102. The centrate outlet tube extends in coaxially surrounding relation to the suspension inlet tube in a manner similar to that previously discussed. The rotatable top 104 of the fluid-containing separation chamber is in operative connection with at least one seal 106 which operates to fluidly seal the cavity of the core with respect to the inlet and outlet pipes. The at least one seal 106 operatively extends in sealing relationship between the outer annular surface of the stationary centrifuge outlet tube 102 and the rotatable top 104 of the core having an upper inner wall that bounds the chamber 90 internally as shown.

In this exemplary arrangement, the inlet tube 92 is fluidly connected to a pump 108. In one exemplary arrangement, the pump 108 is a peristaltic pump that efficiently pumps the cell suspension without causing damage thereto. Of course, it should be understood that this type of pump is exemplary, and in other arrangements, other types of pumps may be used. Further, in this exemplary arrangement, pump 108 is reversible. This enables pump 108 to act as a feed pump to enable pumping of the cell suspension from inlet line 110 into the inlet tube at a controlled rate. Further, in this exemplary arrangement, the pump 108 may be operated as a concentrate removal or discharge pump after the cell concentrate has been separated by centrifugation. In performing this function, the pump 108 operates to pump the cell concentrate out of the separation chamber by reversing the flow of material in the inlet tube 92 with the flow when feeding the cell suspension into the separation chamber. The cell concentrate is then pumped to the concentrate line 112. As shown in fig. 19, the inlet line 110 and the concentrate line 112 may be selectively opened and closed by

valves

114 and 116, respectively. In the exemplary embodiment,

valves

114 and 116 include pinch valves that open and close flow through flexible lines or tubing. Of course, it should be understood that this method is exemplary, and in other arrangements, other methods may be used.

In the exemplary system, the centrate outlet tube 102 is fluidly connected to a centrate discharge line 118. The centrifuge discharge line is fluidly connected to a centrifuge discharge pump 120. In this exemplary arrangement, the centrate discharge pump 120 is a variable flow pump that can selectively adjust its flow rate. For example, in some exemplary arrangements, the pump 120 may comprise a peristaltic pump including a motor whose speed may be controlled to selectively increase or decrease the flow rate through the pump. The outlet of the centrate discharge pump delivers the processed centrate to a suitable collection chamber or other processing means.

In the exemplary arrangement schematically represented in fig. 19, the pressure damping reservoir 122 is fluidly connected to the centrate discharge line 118, which is fluidly intermediate the centrate outlet tube 102 and the pump 120. In this exemplary arrangement, the pressure damping reservoir includes a generally vertically extending container having an interior region configured to contain the liquid centrate in fluid-tight relation. The pressure damping reservoir includes a bottom port 124 fluidly connected to the centrate discharge line 118.

On the opposite side of the reservoir 122 is a top port 126. The top port is exposed to air pressure. In this exemplary arrangement, the top port is exposed to air pressure from a high air pressure source, schematically indicated at 128. In this exemplary embodiment, the high pressure source may include a compressor, air accumulator, or other suitable device for providing a source of high air pressure above atmospheric pressure within a desired range for system operation. Air from high pressure source 128 passes through a sterile filter 130 to remove impurities therefrom. The regulator 132 is operable to maintain a substantially constant air pressure level above atmospheric pressure at the top port of the pressure damping reservoir. In an exemplary arrangement, the air pressure regulator includes an electronic quick-acting regulator to help ensure a substantially constant air pressure is maintained at a desired level. The example fast-acting regulator 132 operates to rapidly increase the pressure acting at the top port 126 when the pressure drops below a desired level, and to rapidly decrease the pressure through the regulator if the pressure acting at the top port is above the set point of the regulator.

In some embodiments, the regulator outlet may also be operatively fluidly connected to the interior of the top 104 of the separation chamber by an air line 143, shown schematically in phantom. In this exemplary arrangement, the outlet pressure of the regulator acting on the top port 126 of the accumulator also acts through the air line 143 on a pocket of air inside the separation chamber that extends down to the level in the chamber above the centripetal pump inlet and on the inside of the at least one seal 106 and radially from a region adjacent the axis 84 to the upper inner wall on the inside of the top 104. In this exemplary arrangement, line 143 applies positive pressure to the area within the separation chamber below the at least one seal through at least one isolation channel extending through the fixed structure of the assembly comprising centrifuge outlet tube 102 and inlet feed tube 92. The at least one example isolation channel of air line 143 applies air pressure to the interior of top 104 through at least one air opening 145 to the separation chamber. The exemplary at least one opening 145 is positioned outside of the outer surface of the outlet tube 102, above the inlet 98 of the centrifugal pump and below the at least one seal 106. Of course, it should be understood that this described structure for an exemplary air line providing positive air pressure to the air pocket in the separation chamber and on the inside of the at least one seal is exemplary, and in other embodiments, other structures and methods may be used.

In the exemplary arrangement of the pressure damping reservoir 122, the upper level sensor 134 is configured to sense liquid centrate inside the pressure damping reservoir. The upper level sensor is operable to sense liquid at an upper level. The lower level sensor 136 is positioned to sense liquid in the reservoir at a lower level. A high level sensor 138 is positioned to detect a high level in the reservoir above the upper level. The high level sensor is positioned to sense a liquid level at an unacceptably high level in order to indicate an abnormal condition that may require shutting down the system or taking other appropriate safety measures. In this exemplary arrangement, the

level sensors

134, 136 and 138 comprise capacitive proximity sensors adapted to sense the level of liquid centrate adjacent thereto within the pressure damping reservoir. Of course, it should be understood that these types of sensors are exemplary, and in other arrangements, other sensors and methods may be used.

The exemplary embodiment also includes other components as long as they can be adapted for the operation of the system. This may include other valves, lines, pressure connections, or other suitable components to perform the processing and manipulation of the suspension, centrate, and concentrate as required by the particular system. This may include additional valves, such as the schematically illustrated valve 140, for controlling the open and closed state of the centrate discharge line 118. The additional lines, valves, connections, or other items included may vary depending on the nature of the system.

The exemplary system of fig. 19 also includes at least one control circuit 142, which may alternatively be referred to as a controller. The exemplary at least one control circuit 142 includes one or more processors 144. The processor is operatively connected to one or more data stores, schematically indicated at 146. As used herein, a processor refers to any electronic device configured to operate via processor-executable instructions to process data stored in the one or more data stores or received from an external source, parse information, and provide output that may be used to control other devices or perform other actions. The one or more control circuits may be implemented as hardware circuits, software, firmware, or applications that are operable to enable the control circuits to receive, store, or process data and perform other actions. For example, the control circuitry may include one or more of a microprocessor, CPU, FPGA, ASIC, or other integrated circuit or other type of circuit capable of performing functions in the manner of an electronic computing device. Further, it is understood that the data storage may correspond to one or more of volatile or non-volatile memory devices such as RAM, flash memory, hard drives, solid state devices, CDs, DVDs, optical storage, magnetic storage, or other circuit-readable media or media on which computer-executable instructions and/or data may be stored.

The circuit-executable instructions may include instructions in any of a variety of programming languages and formats, including but not limited to routines, subroutines, programs, threads of execution, objects, methods, and functions to perform actions such as those described herein. The structure of the control circuit may include, correspond to, and utilize the principles described in Ramesh s. ganker's textbook entitled "microprocessor architecture, programming and applications" of model 8085 (prentic Hall, 2002), which is incorporated herein by reference in its entirety. Of course, it should be understood that these control circuit configurations are exemplary, and in other embodiments, other circuit configurations for storing, processing, parsing, and outputting information may be used.

In this exemplary arrangement, the at least one control circuit 142 is operatively connected with at least one sensor (e.g.,

sensors

134, 136 and 138) via a suitable interface. The at least one control circuit is also operatively connected to the variable flow rate discharge pump 120. Further, in some exemplary embodiments, the at least one control circuit may also be operatively connected with other devices, such as the motor 86, the pump 108, the regulator 132, the pneumatic pressure source 128, fluid control valves, and other devices.

The exemplary at least one control circuit is operable to receive data and control these devices in accordance with circuit-executable instructions stored in the data storage 146. In this exemplary arrangement, fluid level 147 in the fluid damping reservoir is a property that corresponds to the pressure in centrifuge discharge tube 102. In one exemplary implementation that does not utilize air line 143, the fact that the pressure in the centrate discharge tube indicates the nature of the pressure in the top 104 of the core and the pressure in the separation chamber adjacent seal 106 is used to control the operation of the discharge pump and other components. As previously mentioned, it is desirable to maintain a positive pressure above atmospheric pressure and a pocket of air adjacent the at least one seal within the separation chamber to avoid introduction of contaminants into the separation chamber that may result from the negative pressure. However, if the fluid level within the separation chamber becomes too high, the pressure and suspension material being processed may spill over the seal, which may result in potential contamination and undesirable exposure and loss of processed material. This may be due to a situation where the back pressure on the centrifuge line connected to the outlet of the centripetal pump is too high.

In this exemplary arrangement, the drum speed produces a corresponding pumping force and pump output pressure level for the centripetal pump. This pump output pressure level of the centripetal pump varies with the rotational speed of the drum and the core. An exemplary arrangement that does not use air line 143 provides a controlled back pressure on the centrate outlet tube. The back pressure is provided by controlling the speed of the motor operating the pump 120 and the level 147 of the fluid in the pressure damping reservoir. The back pressure is maintained at a level less than the pump output pressure (so that the centripetal pump can convey the centrate out of the separation chamber), but at a positive pressure above atmospheric pressure to ensure that contaminants do not penetrate through the seal into the separation chamber, and so that air at elevated pressure is maintained inside the separation chamber adjacent the seal to isolate the seal from the components of the suspension being processed.

In this exemplary arrangement, the elevated pressure applied to the top port 126 of the pressure damping reservoir is maintained by the regulator 132. Further, the speed of pump 120 is controlled by the at least one control circuit 142 to maintain the liquid level 147 between the upper and lower liquid levels 136 sensed by sensor 134, controlling the flow of centrate out of the separation chamber such that the pressure in the top region of the separation chamber is maintained at a desired constant value and the centrate does not contact or spill the seal.

In an alternative embodiment using air line 143, the positive pressure water level of the regulator acts on the fluid in reservoir 122 and on the area of the separation chamber above the centrifugal pump inlet. Since the positive pressure level of the air applied at both locations is the same, the back pressure on the centrate discharge line (which is the pressure applied above the fluid in the reservoir) is in fact always the same as the pressure in the air pocket at the top of the separation chamber. This enables the centripetal pump to operate without any net effect from either pressure.

In this exemplary embodiment, the pump 120 and other system components are controlled in response to the at least one control circuit 142 to ensure that a sufficient volume of air is always present inside the reservoir 122 during the production of the centrate. This ensures that the reservoir provides the desired damping effect on changes in centrifuge discharge line pressure that might otherwise be caused by the pumping action of the pump 120. This is accomplished by maintaining the liquid in the reservoir 122 at a level no higher than the upper level detected by the sensor 134. In addition, the liquid level in the reservoir is controlled to remain above the lower liquid level sensed by sensor 136. This ensures that the centripetal pump does not pump air and aerate the centrifuge.

In this exemplary arrangement, the flow of centrate exiting the separation chamber is controlled by operation of the at least one control circuit. The exemplary control circuit may operate the system during processing conditions to maintain a flow of cell suspension through pump 108 into separation chamber 90 at a substantially constant rate, while the separation process occurs with motor 86 operating to maintain a constant drum speed to effect separation of the centrate and cell concentrate. The exemplary arrangement also operates to maintain a desired constant back pressure from the centrifugal pump on the centrifuge discharge line while maintaining the air in the separation chamber above the level of the underside of the air pocket to isolate the at least one seal 106 from the centrifuge and concentrate materials being processed.

In one exemplary arrangement, the pressure maintained in the pressure damping reservoir by operation of the regulator is set to about 2kPa (0.29psi) above atmospheric pressure. In this exemplary system, this pressure has been found to be suitable to ensure that seal integrity and isolation is maintained during all stages of cell suspension processing. Of course, it should be understood that this value is exemplary, and in other arrangements, other pressure values and pressure damping reservoir configurations, sensors, and other features may be utilized.

Fig. 20 schematically illustrates exemplary logic performed by operation of the at least one control circuit 142 in connection with maintaining a desired pressure level in the centrate discharge tube and within the top of the separation chamber. It should be understood that in some exemplary embodiments, the control circuit may perform many additional or different functions in addition to those shown. In addition to the pressure control functions, these functions may include overall control of the various processes and steps for centrifuge operation. As shown in fig. 20, in an initial subroutine step 148, the at least one control circuit 142 is operable to determine whether centrifuge operation is currently in a mode in which the centrate is being discharged from the separation chamber. If so, the at least one control circuit is operable to cause the centrate discharge pump 120 to operate to discharge the delivered centrate through the centrate discharge line 118. This may be done by operating the motor of the pump. In this exemplary arrangement, the flow rate of the pump 120 may be initially a set value, or alternatively may vary depending on the particular operating conditions determined by the control circuit operation during the process. Operation of the centrate discharge pump is represented by step 150.

Then, in step 152, the at least one control circuit is operable to determine whether liquid is sensed at a high level of the high level sensor 138. If so, this represents an undesirable condition. If liquid is sensed at the level of sensor 138, the control circuitry operates to take action to address the condition. This may include operating the pump 120 to increase its flow rate and making a subsequent determination if the liquid level drops for a period of time while the centrifuge continues to operate. Alternatively or additionally, the at least one control circuit may reduce the speed of the pump 108 to reduce the flow of the incoming material. If such action does not result in the liquid level falling within a set period of time, additional steps are taken. These steps may also include slowing or stopping the rotation of the drum 182. These actions may also include stopping operation of pump 108 to avoid introducing more suspension material into the separation chamber. These steps, which are commonly referred to as shutting down the normal operation of the system, are represented by step 154.

If no liquid is sensed at the level of high level sensor 138, the at least one control circuit is next operable to determine if liquid is sensed at the upper level of sensor 134. This is represented by step 156. If liquid is sensed at the upper level sensor, the at least one circuit operates in response to its stored instructions to increase the speed of the drain pump 120 and thus its flow rate. In one exemplary embodiment, this is accomplished by increasing the speed of a motor that is part of the pump. This is represented by step 158. Increasing the flow rate of the pump causes the liquid level 147 in the pressure damping reservoir to begin to decrease as the pump 120 moves more liquid.

If no liquid is sensed at the upper level of the sensor 134 in step 156, the at least one control circuit then operates to determine if no liquid is sensed at the lower level of the sensor 136. This is represented by step 160. If the level is not at the level of sensor 136, the control circuitry operates according to its programming to control pump 120 to reduce its flow rate. In one exemplary embodiment, this is accomplished by slowing the speed of the motor. This is represented by step 162. In this exemplary arrangement, slowing the flow rate of the pump 120 causes the liquid level 147 to begin to rise in the pressure damping reservoir. In some exemplary arrangements, if the liquid level within the reservoir does not rise within a given time, the control circuitry may operate according to its programming to cause additional actions, such as those associated with the shutdown step 154 discussed previously. The control circuitry of the exemplary embodiment is operable to vary the pumping rate of pump 120 to maintain the liquid level 147 within the pressure damping reservoir at a substantially constant level between the levels of

sensors

134 and 136 during production of the centrate.

In this exemplary arrangement, maintaining a substantially constant elevated pressure of sterile air above the liquid in the pressure damping reservoir helps ensure that a similar elevated pressure is consistently maintained in the centrate outlet line and at the seal within the separation chamber. Furthermore, in this exemplary arrangement, the pressure is enabled to be controlled at a desired level during different operating conditions of the centrifuge during which the bowl is rotated at different speeds. This includes, for example, conditions during which the separation chamber is initially filled at a relatively high rate by introducing the cell suspension, and during which the centrifuge is rotated at a relatively low speed. During subsequent final filling conditions, the pressure may also be maintained, wherein the flow rate of the cell suspension into the separation chamber occurs at a slower rate, and during this the rotational speed of the drum is increased to a higher rotational speed. Furthermore, during feeding of the suspension into the drum and during discharge of the centrate from the separation chamber, a positive pressure is maintained as previously discussed. Further, in an exemplary embodiment, the at least one control circuit may be operable to maintain a positive pressure also during the period of time that the concentrate is removed by pumping the concentrate out of the separation chamber. Maintaining a positive pressure within the separation chamber during all of these conditions reduces the risk of contamination and other undesirable conditions that might otherwise arise due to negative (sub-atmospheric) pressure conditions.

Of course, it should be understood that the features, components, structures, and control methods are exemplary and in other arrangements, other methods may be used. Furthermore, although the exemplary arrangement includes a system that operates in a batch mode rather than a mode in which both the centrate and concentrate are processed continuously, the principles thereof may also be applied to such other types of systems.

While an exemplary embodiment of a pressure damping reservoir may be used to help ensure that a desired pressure level is maintained in the outlet tube and the separation chamber, other methods may be utilized in other exemplary embodiments. For example, in some arrangements, the pressure may be sensed and/or applied directly in the outlet tube, in the separation chamber, or in other locations corresponding to the pressure in the separation chamber. In some arrangements, the flow rate of the discharge pump may be controlled in order to maintain a suitable pressure level. In other arrangements, the exemplary control circuitry may be operative to control the discharge pump and the pump that supplies the suspension into the core and/or appropriate valves or other flow control devices in order to maintain the appropriate pressure levels. Such alternative methods may be required depending on the particular centrifuge apparatus used and the type of material being processed.

Fig. 21 schematically shows an alternative centrifuge system 170 specifically configured to continuously or semi-continuously separate cells in a cell culture batch into a cell centrifuge and a cell concentrate. The exemplary system shows a rigid centrifuge bowl 172 that is rotatable about an axis 174. The drum includes a cavity 176 configured to releasably receive a single-use configuration 178 therein. The rigid drum includes an upper opening 180. An annular securing ring or other securing structure, schematically indicated at 182, enables the single-use structure 178 to be releasably secured within the drum cavity.

The exemplary single-use construction 178 of this exemplary embodiment includes a central axially extending feed tube 184. As discussed later, the feed tube is used to deliver the cell culture batch material into the interior region 186 of the single-use construction 178. The feed tube 184 extends from an upper portion at the first axial end 188 of the single-use set to an opening 190 in an interior region at a lower portion at the second axial end 192. The single-use construction 178 includes a substantially disc-shaped portion 194 adjacent the first axial end. The exemplary disc portion 194 is substantially rigid, meaning that it is rigid or semi-rigid, and includes an annular outer periphery 196. The annular outer periphery is configured to engage an upper annular bounding wall 198 of the centrifuge bowl chamber 176. The annular outer periphery of the disc portion 194 is configured to engage the rigid drum 172 such that the single-use configuration rotates therewith.

The exemplary single-use construction 178 also includes a hollow rigid or at least semi-rigid cylindrical core 200. The core 200 is operatively engaged with the disc portion 194 and rotatable therewith. The core 200 is axially aligned with the disk portion and extends axially intermediate the upper and lower portions of the single-use configuration. The core 200 includes an upper opening 202 and a lower opening 204 through which the feed tube 184 extends.

The disk portion 194 includes a substantially circular centrifuge-to-heart chamber 206. A centrifuge centripetal pump 208 is positioned in the pump chamber 206. A substantially annular centrifuge opening 210 is fluidly connected to the centrifuge pump chamber 206. By substantially annular it is meant that the opening may consist of an annular arrangement of discrete openings as well as a continuous opening. The centrate feed pump 208 is fluidly connected to a centrate discharge tube 212. The centrate discharge tube 212 extends in coaxial surrounding relationship with the feed tube 184. The discharged centrifuge passes through a substantially annular opening in the centrifuge towards the periphery of the centrifugal pump and through an annular space in the centrifuge discharge tube 212 outside the feed tube.

The disc portion 194 also includes a concentrate centripetal pumping chamber 214. The concentrate centripetal chamber 214 is a substantially circular chamber positioned above the centrifuge centripetal chamber 206. The concentrate centripetal chamber 214 has a concentrate centripetal pump 216 positioned therein. The concentrate radial pump is fluidly connected to the concentrate discharge line 220. The concentrate discharge tube 220 extends in an annular relationship around the centrifuge discharge tube 212. The concentrate passes through a substantially annular opening at the periphery of the concentrate centripetal pump and through an annular space in the concentrate discharge tube 220 outside the centrifuge discharge tube.

A substantially annular concentrate opening 218 is fluidly connected to the concentrate pump chamber 214. In this exemplary arrangement, the substantially annular concentrate opening and the substantially annular centrifuge opening are concentric coaxial openings, wherein the concentrate opening is disposed radially outside of the centrifuge opening. Of course, this arrangement is exemplary, and in other embodiments, other methods and configurations may be used.

The exemplary single-use construction 178 also includes a flexible outer wall 222. The flexible outer wall 222 is a fluid tight wall that extends in operative supporting engagement with the wall defining the rigid drum cavity 176 in the operative position of the exemplary single use configuration 178. In this exemplary arrangement, the flexible outer wall 222 is operatively engaged with the disc portion 194 in a fluid tight connection. The flexible outer wall has an inner frustoconical shape with a smaller inner radius adjacent a lower portion of the single-use construction adjacent the second axial end 192.

The example flexible outer wall 222 extends in surrounding relation to at least a portion of the core 200. The wall 222 also defines an annular separation chamber 224. A separation chamber 224 extends radially between the outer wall of the core 200 and the flexible outer wall 222. The substantially annular concentrate opening 218 and the substantially annular centrifuge opening 210 are each in fluid communication with a separation chamber 224.

In this exemplary arrangement, the flexible outer wall 222 has a textured outer surface 226. The textured outer surface is configured to enable air to escape from the space between the surface defining the cavity of the rigid drum 172 and the flexible outer wall 222. In one exemplary arrangement, the textured outer surface may comprise substantially the entire area of the flexible outer wall in contact with the rigid drum. In an exemplary arrangement, the textured outer surface may include a pattern of one or more outwardly extending protrusions or dimples 228 with spaces or recesses therebetween to facilitate the passage of air. When the single-use configuration 178 is positioned in the drum cavity 176, air can be expelled therefrom through the upper opening 180 or through the lower opening 230. In an exemplary arrangement, the projections may be constructed of an elastically deformable material that may decrease in height in response to the force of the liner against the rigid wall of the drum. The textured outer surface 226 of the flexible outer wall 222 reduces the risk that air pockets will become trapped between the rigid bowl of the centrifuge and the single-use configuration. Such cavitation may cause irregularities in the wall profile, which may cause imbalances and/or alter the profile of the separation chamber in a manner that adversely affects the separation process. Of course, it should be understood that the air release structure is exemplary and other embodiments of other air release structures may be used.

The exemplary single-use configuration shown in fig. 21 also includes a rigid or semi-rigid lower disk portion 232. Rigid or semi-rigid materials operate to maintain their shape during operation. In this exemplary arrangement, the lower disc portion 232 has a tapered shape and is operatively attachedly connected with the lower end of the core 200 by a vertically extending wall or other structure. A plurality of angularly spaced fluid passages 234 extend between the upper surface of the disc portion 232 and a radially outwardly lower portion of the core. The fluid channel 232 extends radially outward and upward relative to the bottom of the second axial end 192 and enables cells in the cell culture batch material entering the interior region 186 through the opening 190 in the feed tube 184 to enter the separation chamber 224 radially outward and upward.

In this exemplary arrangement, the flexible outer wall 222 extends below the lower disk portion 232 at the second axial end 192 of the single-use construction. The flexible outer wall 222 extends intermediate the lower disk portion 232 and a wall surface of the rigid drum 172 that defines a cavity in which the single-use configuration is positioned.

In this exemplary arrangement, the feed pipe 184, the centrate discharge pipe 212 and the concentrate discharge pipe 220, as well as the centrate centrifugal pump 208 and the concentrate centrifugal pump 216, remain stationary while the centrifuge bowl 172 and the upper disc portion 194, the lower disc portion 232 and the flexible outer wall 222 rotate relative to the bowl. At least one annular resilient seal 236 operatively extends between the outer surface of the concentrate outlet conduit 220 and the upper disc shaped portion 194 in sealing engagement. The at least one seal 236 maintains a hermetic seal in a manner similar to that previously discussed such that air pockets may be maintained in the interior region 186 during cell processing to isolate the seal from the cell culture batch material being processed. The air pockets maintained within the interior region of the single-use configuration are configured such that the centrifuge centripetal pump 208 and the concentrate centripetal pump 216 remain in fluid communication with the cell culture batch material. In a manner similar to that previously discussed, a positive pressure may be maintained within the interior region to ensure that an air pocket exists to sufficiently isolate the at least one seal 236 from the cell culture batch material being processed. Alternatively, other methods may be used in order to keep the seal isolated from the material being processed.

The exemplary system 170 operates in a manner similar to that previously discussed. Cells in a cell culture batch material are introduced through the feed tube 184 into the interior region 186 of the single-use construction 178. Cells enter the interior region 186 through a feed tube opening 190 at the lower axial end of the single-use configuration. Centrifugal forces cause the cells to move outward through the opening 234 and into the separation chamber 224. The outwardly and upwardly tapering outer wall 222 causes cells or cell material containing cell concentrate to collect near the radially outward and upper region of the separation chamber 224. The typically cell-free centrate collects in the separation chamber, radially inwardly adjacent the outer wall of the core 200.

In this exemplary arrangement, the cell centrate passes upwardly through a substantially annular centrate opening into the centrate pump chamber. The centrate passes inwardly through a substantially annular opening of the centrate centrifugal pump and then upwardly through the centrate discharge tube 212. At the same time, the cell concentrate passes through the substantially annular concentrate opening 218 and into the concentrate centripetal chamber 214. The cell concentrate passes inwardly through a substantially annular opening of the concentrate centripetal pump 216 and then upwardly through the concentrate discharge tube 220. This exemplary configuration enables the exemplary system 170 to operate on a continuous or semi-continuous basis. Operation of the system 170 may be controlled in a manner similar to that discussed later in order to facilitate reliable prolonged operation of the system and delivery of the desired cell concentrate and generally cell-free centrate in separate output fluid streams.

Fig. 22 illustrates an alternative centrifuge system, generally designated 238. The system 238 has a single-use configuration 240. The single-use construction 240 is similar in most respects to the single-use construction 178 described previously. Some of the structures and features of the single-use structure 240 that are substantially identical to those described in connection with the single-use structure 178 are labeled with the same reference numerals as those used to describe the single-use structure 178.

The single-use construction 240 differs from the single-use construction 178 in that it includes a rigid or semi-rigid lower disk portion 242. The lower disk portion 242 is a generally conical structure operatively connected to the lower end of the core 200. A plurality of radially outwardly and upwardly extending fluid passages 244 extend between the lower end of the core 200 and the lower disk portion 242. The exemplary lower disk portion 242 also includes a plurality of angularly spaced radially extending vanes 246. The fluid passages extend radially outwardly between each angularly adjacent pair of vanes 246. In this exemplary arrangement, the vanes 246 extend upwardly from the bottom of the disc portion 242, and at least some of the vanes are operatively engaged with the core at a radially outer portion thereof. In this exemplary arrangement, the blades 246 accelerate the cell culture batch to facilitate movement and separation within the interior region of the single-use configuration.

An alternative exemplary embodiment of a centrifuge system 248 is shown in fig. 23. This exemplary embodiment includes a single-use configuration 250. The single-use construction 250 is similar in many respects to the previously described single-use construction 178. Some structures and features similar to those described above in the single-use structure 178 are labeled with the same reference numerals on the single-use structure 250.

The exemplary single-use construction 250 differs from the single-use construction 178 in that it includes a lower disc-shaped portion 252. The lower disk portion 252 is a rigid or semi-rigid conical structure that is operatively attached to the core 200 via a wall or other suitable structure. The lower disk portion 252 includes a plurality of angularly spaced radially outwardly extending accelerator blades 254. The accelerator blades 254 extend downward from the lower tapered side of the disk portion 252. Each angularly adjacent pair of vanes 254 has a fluid passage extending therebetween. In this exemplary arrangement, the flexible outer wall 222 extends in an intermediate relationship between the lower ends of the vanes 254 and the walls of the rigid drum 172 that define the cavity 176. This exemplary configuration provides a submerged accelerator operable to accelerate a cell culture batch material to facilitate its separation within an interior region of a single-use configuration. Of course, it should be understood that the single-use structural features described herein may be combined in different arrangements to facilitate separation of different types of materials and substances having different properties and achieve a desired output fluid flow.

Fig. 26 shows an alternative single-use configuration 304. The single-use configuration 304 is similar to the previously described single-use configuration 178, except as otherwise noted herein. Elements that are identical to elements in the single-use configuration 178 have been designated with the same reference numerals in fig. 26.

The single-use configuration 304 includes a continuous annular concentrate dam 306. A concentrate dam 306 extends downwardly in the separation chamber 224 and is disposed radially inward of the substantially annular concentrate opening 218. The example annular concentrate dam shown in cross-section extends downwardly below the concentrate opening, and the example annular concentrate dam shown in axial cross-section includes a tapered outer surface 308 extending outwardly and toward the opening 218.

The single-use construction 304 also includes a continuous annular centrifuge dam 310. A centrifuge dam 310 extends downwardly in the separation chamber 224 below the substantially annular centrifuge opening 210. The centrifuge dam 310 is disposed radially outward from the centrifuge opening 210. In this exemplary arrangement, the downward distance that concentrate dam 306 and centrate dam 310 extend in separation chamber 224 is substantially the same. However, in other exemplary arrangements, other configurations may be used. Also, in other exemplary arrangements, the centrifuge structure may include a concentrate dam or a centrifuge dam, but not both.

An annular recess 312 extends radially in the separation chamber between the centrifuge dam and the concentrate dam. The exemplary annular recess extends upwardly between the centrifuge dam and the concentrate dam to form an annular air pocket therebetween.

In an exemplary embodiment, the concentrate dam 306 helps ensure that primary cellular material or other solid material to be separated can pass outwardly along the upper portion defining the separation chamber 224 to reach the concentrate opening 218 and concentrate centripetal chamber 214. The centrifuge dam 310 also helps ensure that predominantly cell-free centrifuge material is allowed to pass along the upper surface bounding the separation chamber 224 and into the substantially annular centrifuge opening 210 to reach the centrifuge pump chamber 206. It should be understood that a variety of different configurations of concentrate dams and centrifuge dams may be used in different example arrangements depending on the nature of the material being processed and the requirements for processing such material.

FIG. 24 is a schematic diagram of an exemplary control system for providing substantially continuous processing of cell culture material to produce a generally cell-free centrifuge stream and a cell concentrate stream. In this exemplary arrangement, the centrifuge system 170 previously discussed is shown. However, it should be understood that this exemplary system feature may be used with many different types of materials and centrifuge systems and structures, such as those discussed herein.

In the exemplary embodiment shown, centrifuge bowl 172 is rotated about axis 174 by motor 256 at a selected speed. Feed line 184 is operatively connected to cell culture feed line 258 through which cell culture batch material is received. The feed line is operatively connected to a feed pump 260. In an exemplary arrangement, the feed pump 260 can be a peristaltic pump or other suitable pump for delivering cell culture at a selected flow rate into a single-use configuration.

The centrate discharge tube 212 is fluidly connected to the centrate discharge line 262. A centrate optical density sensor 264 is operatively connected to an interior region of the centrate discharge line 262. In this exemplary arrangement, the centrifuge optical density sensor is an optical sensor operable to determine the density of cells currently in the centrifuge passing from the single-use configuration. In this exemplary embodiment, this is achieved by measuring a decrease in intensity of light output by the emitter that is received by a receiver disposed from the emitter and has at least a portion of the centrate stream passing therebetween. The amount of light from the emitter received by the receiver decreases as the density of the cells in the centrate increases. Of course, this is only one example of a sensor that can be used to determine the density or amount of cells present in the centrate, and in other arrangements, other types of sensors may be used. For example, the light may be near infrared light or other visible or invisible light. In other sensing arrangements, other forms of electromagnetic, acoustic, or other types of signals may be used for sensing. The centrifuge discharge line is also operatively connected to a centrifuge pump 266. In this exemplary embodiment, the centrifuge pump may comprise a peristaltic pump or other variable rate pump suitable for pumping centrifuge material.

In this exemplary arrangement, the concentrate drain 220 is operatively connected to the concentrate drain line 268. A concentrate optical density sensor 270 is operatively connected to at least a portion of the interior region of the concentrate discharge line 268. The exemplary concentrate optical density sensor may operate in a similar manner to the centrifuge optical density sensor previously discussed. Of course, it should be understood that the concentrate optical density sensor may comprise different structures or properties, and that different types of cell density sensors may be used in other exemplary embodiments. The concentrate discharge line 268 is operatively connected to a concentrate pump 272. In this exemplary embodiment, the concentrate pump 272 may include a peristaltic pump or other variable rate pump suitable for pumping concentrate without causing damage thereto. Of course, it should be understood that these structures and components are exemplary and that alternative systems may include different or additional components.

The exemplary control system includes a control circuit 274, which is alternatively referred to herein as a controller. In an exemplary embodiment, the control circuitry may include one or more processors schematically indicated at 276. The control circuitry may also include one or more data memories, schematically indicated at 278. The one or more data stores may include one or more types of tangible media that hold circuit-executable instructions and data that, when executed by a controller, cause the controller to perform operations such as those discussed later herein. Such a medium may include, for example, solid-state memory, magnetic memory, optical memory, or other suitable non-transitory medium for holding circuit-executable instructions and/or data. The control circuit may include structures similar to those previously discussed.

The operations performed by the exemplary controller 274 will now be described in connection with the schematic representation of a logic flow illustrated in FIG. 25. In this exemplary arrangement, the controller 274 is operable to control the operation of the components in the system so as to maintain the concurrent delivery of the output flow of the generally cell-free centrate and cell concentrate. This is accomplished by using optical density sensors in the respective centrifuge and concentrate outlet lines to detect the cell density (or turbidity) of the output feed and adjust the operation of the system components to maintain the output within a desired range.

In using this exemplary control system, the cell concentration of the cells in the cell culture material to be processed is measured separately before operation of the system is initiated. The desired axial rotational speed of the centrifuge is determined as the speed for operating the feed pump 260. In this exemplary arrangement, the rotational speed of the centrifuge and the feed rate of cellular material through the feed pump are typically maintained by a controller at constant set points. Of course, in other arrangements and systems, alternative methods may be used, wherein the speed and feed rate may be adjusted by the controller during cell processing.

In this exemplary arrangement, the discharge rate (flow rate) of the external concentrate pump 272 is set at an initial value, referred to herein as the "primary value," based on the determined cell concentration. Also preset in this exemplary embodiment is a "priming duration" which corresponds to the period of time that the external concentrate pump 272 will initially operate at a priming value. This duration allows the single-use construction 178 to be partially filled. Also in this exemplary system, a "base speed" is set for the concentrate pump based on the cell density and the feed rate from the feed pump 260. The base speed of the concentrate pump is the speed (which corresponds to the flow rate) at which the concentrate pump will operate after the priming duration. In this exemplary arrangement, the set base speed is generally expected to correspond to a concentrate pump speed that will produce a centrate having a cell density below a desired set limit and a cell concentrate having a cell density generally above another desired set limit. The set point and limit values are received by the controller in response to input through a suitable input device and stored in the at least one data store.

In the exemplary logic flow illustrated in fig. 25, step 280 represents operation of the concentrate pump 272 at an initial priming rate. At step 282, it is determined by the controller whether the concentrate pump has been operating at the priming rate for a period of time corresponding to the duration of priming that is operable to at least partially fill the single-use configuration 178.

Once the concentrate pump has operated at the priming speed for the priming duration, the controller causes the concentrate pump speed to then increase to the base speed, as represented by step 284. Controller 274 operates to monitor the cell density in the centrate as detected by sensor 264. The controller operates to determine if the optical density is above the desired set point, as shown at step 286. If the optical density of the centrate is not above the set point, the centrate is sufficiently free of cells or cellular material such that this measurement does not cause the controller to change the operating speed of the concentrate pump, and the logic returns to step 284.

If it is determined in step 286 that the optical density of the centrate is above the set point, the logic proceeds to step 288. In step 288, control operates to increase the speed of the concentrate pump by the set incremental step amount. This increase in velocity step is generally intended to result in an optical density of cleared centrate due to a reduction in the number of cells therein.

After increasing the speed of the concentrate pump 272 in step 288, the controller then operates in response to the sensor 264 to determine in step 290 whether the optical density of the centrate is still above the set point at a set time after the incremental increase in the speed (flow) of the concentrate pump. If so, the controller continues to monitor the optical density of the centrate until it is not above the set point. In this exemplary arrangement, the instructions include a set period of time during which the centrate optical density must not be above the set point before the concentrate pump speed controller determines that the adjustment to the base speed is sufficient to maintain the optical density of the centrate at a level at or below the desired set point. Step 292 represents the controller determining that the increased concentrate pump speed has maintained the optical density of the centrate at or below the set point of the stored set time period value corresponding to consistently producing a completely cell-free centrate outflow or reaching a programmed wait time. In response to producing a completely cell-free centrate for a desired duration or for a programmed wait time, the controller next operates in step 294 to cause the basal speed value of the concentrate pump to be adjusted to correspond to an increased basal speed. The controller sets a new base speed and the logic returns to step 284. It should be noted that if the centrifuge optical density is still above the set point as determined in step 286, the concentrate pump speed will again be adjusted.

The exemplary controller also simultaneously monitors the optical density of the cells in the output concentrate stream. This is done by monitoring the optical density as detected by sensor 270. The controller operates to determine if the optical density in the concentrate is below a desired set point, as shown in step 296. If the concentrate optical density is detected at or above the desired set point value stored in the data store, the concentration of cells in the concentrate output stream is at or above the desired level, and the logic returns to step 284. However, if the optical density of the concentrate is below the desired set point, meaning that the level of cells in the concentrate is below the desired level, the controller moves to step 298. In step 298, the speed of the concentrate pump is reduced by a predetermined incremental step amount. Reducing the speed of the concentrate pump will reduce the output flow rate, typically increasing the amount of cells in the concentrate output stream, and thus increasing the optical density of the concentrate output stream.

The controller then operates the concentrate pump 272 at the new reduced speed, as shown in step 300. As shown in step 302, the controller operates the concentrate pump at this reduced rate for a set period of time corresponding to the set point stored in the data storage device so that the concentration of cells in the output concentrate stream can be increased before determining whether the rate reduction is sufficient. Once it is determined in step 302 that the time period has elapsed, the controller returns to step 284 from which the logic flow is then repeated to determine whether further speed adjustments are needed.

Of course, it should be understood that this schematic simplified logic flow is exemplary, and in other embodiments, different logic flows and/or additional operating parameters of system components may be monitored and adjusted to achieve desired output flows of centrate and centrate. For example, in other exemplary arrangements, the speed of the centrate discharge pump, and thus the centrate discharge flow rate, may be varied by the controller at least partially in response to the optical density corresponding to the level of cells in the centrate detected by the centrate optical density sensor. For example, if the cell level in the centrifuge is detected to be above a set limit, the controller may operate to decrease the flow rate of the centrifuge pump. This may be done by the controller instead of or in combination with controlling the concentrate discharge flow rate. The controller can appropriately vary the centrifuge flow rate to ensure that the cell level in the centrifuge remains below a set limit or within a set range.

Alternatively or additionally, the controller may also control the flow rate of the cell suspension into the single-use configuration. This can be done in conjunction with varying the flow rates of the centrate and concentrate from the single-use configuration to maintain the cell levels in the centrate and concentrate within programmed set limits stored in a memory associated with the controller. In addition, the controller may also operate in accordance with its programming to vary other process parameters, such as changes in the rotational speed of the drum, introduction of diluent and diluent introduction rate, and other process parameters, to maintain the centrate and concentrate properties within programmed limits and desired process rates. Further, in other exemplary embodiments, other properties or parameters may be monitored and adjusted by the control system in order to achieve a desired product.

Fig. 27 shows a cross-sectional view of another alternative single-use centrifuge structure 314. The single-use configuration 314 is generally similar to the single-use configuration 178 previously discussed, except as specifically noted. Single-use configuration 314 includes elements operable to help ensure that the air/liquid interface of the air pocket, which extends in the single-use configuration and isolates seal 236 from the material being processed, remains more stably at a desired radial position.

In the single-use configuration 314, the centrifuge pump 208 is positioned in the centrifuge pump chamber 316. Centrifuge pump chamber 316 is bounded vertically at the bottom by a circular lower centrifuge to pump chamber surface 318. The centrifuge pump chamber 316 is vertically bounded on the upper side by a circular upper centrifuge to a centrifuge chamber surface 320.

The lower centrifuge pump chamber surface 318 extends radially outward from the lower centrifuge to the pump chamber opening 322. In this exemplary arrangement, the lower centrifuge to pump chamber opening 322 extends through the circular top of the core 200 and corresponds to the upper opening 202 previously discussed. The feed tube 184 extends through the lower centrifuge to open into the centrifuge chamber.

The upper centrifuge chamber surface 320 extends radially outward from a circular upper centrifuge chamber opening 324. The feed pipe 184 and the centrifuge discharge pipe 212 extend axially through the upper centrifuge to open into the centrifuge chamber.

A plurality of angularly spaced upwardly extending lower centrifuge chamber vanes 326 extend over the lower centrifuge chamber surface 318. Each lower centrifuge chamber vane 326 extends radially outward from the lower centrifuge to the centrifuge chamber opening 322. Lower centrifuge chamber blades 326, shown in more detail in fig. 28, extend radially outward from rotational axis 174 by a lower centrifuge blade distance V. In this exemplary arrangement, the lower centrifuge chamber vanes 326 extend upwardly in circular recesses on the lower centrifuge chamber surface 318. However, it is to be understood that such an arrangement is exemplary and other embodiments of other arrangements may be used; for example, the radial length of the vanes, the vane height, and the depth and diameter of the recess may be varied to achieve desired fluid pressure properties.

A plurality of angularly spaced, downwardly extending upper centrifuge chamber vanes 328 extend from the upper centrifuge to the centrifuge chamber surface 320. Each of the upper centrifuge chamber vanes 328 extends radially outward from the upper centrifuge toward the centrifuge chamber opening 324. The upper centrifuge chamber blades extend radially outward from the axis of rotation 174 an upper centrifuge blade distance. In this exemplary arrangement, the upper centrifuge blade distance substantially corresponds to the lower centrifuge blade distance V. In this exemplary arrangement, the upper centrifuge chamber vanes extend downwardly in a circular recess on the upper centrifuge centripetal pumping chamber surface, the circular recess having a similar configuration to that shown in figure 28 for the lower centrifuge chamber vanes, but in the opposite orientation.

In the exemplary arrangement shown, the centrifuge centripetal pump 208 includes a substantially annular centrifuge centripetal pump opening 330. The substantially annular centrifuge sets the centrifuge pump opening distance radially outward from the axis of rotation 174 towards the centrifuge pump opening 330. For reasons discussed later, the centrifuge pump opening distance at which the centrifuge centripetal pump opening 330 is located is a greater radial distance than the lower and upper centrifuge blade distances.

In the exemplary arrangement of the single-use configuration 314, the concentrate centripetal pump 216 is positioned in the concentrate pump chamber 332. The concentrate pump chamber 332 is vertically bounded on the underside by a circular lower concentrate to a pump chamber surface 334. The concentrate pump chamber 332 is vertically bounded on the upper side by a circular upper concentrate to a pump chamber surface 336.

The lower concentrate pumping chamber surface 334 extends radially outward from a lower concentrate pumping chamber opening 338. In this exemplary arrangement, the lower concentrate pumping chamber opening corresponds in size to and is continuous with the upper concentrate pumping chamber opening 324. The feed tube 184 and the centrate discharge tube 212 extend through the lower concentrate centripetal chamber opening 338.

A plurality of angularly spaced upwardly extending lower concentrate chamber vanes 340 extend over the lower concentrate centripetal chamber surface 334. The lower concentrate chamber vanes 334 extend radially outward from the lower concentrate toward the pumping chamber opening 338. The lower concentrate compartment blades 334 extend radially outward from the axis of rotation by a lower concentrate blade distance. In this exemplary arrangement, the lower concentrate cell vanes 334 extend over circular recesses in the lower concentrate centripetal chamber surface, similar to the upper and lower concentrate cell vanes previously discussed. Of course, it should be understood that this configuration is exemplary.

The upper concentrate pumping chamber surface 336 extends radially outward from the upper concentrate pumping chamber opening 342. The feed pipe 184, the centrifuge discharge pipe 212 and the concentrate discharge pipe 220 extend coaxially through the upper concentrate centripetal chamber opening 342. A plurality of angularly spaced upper concentrate compartment vanes 344 extend downwardly from the surface 336. The upper concentrate chamber vanes extend radially outward from the upper concentrate toward the pumping chamber opening 342 by an upper concentrate vane distance. The upper concentrate chamber vanes extend in upwardly extending circular recesses in the upper concentrate centripetal chamber surface. In this exemplary arrangement, the upper concentrate compartment vanes are constructed in a similar manner to the lower concentrate compartment vanes and the upper and lower centrifuge compartment vanes previously discussed. Of course, it should be understood that this method is exemplary, and other embodiments of other methods may be used.

The concentrate centripetal pump 216 includes a substantially annular concentrate pump opening 346. The concentrate pump opening is radially disposed from the rotational axis 174 by a concentrate pump opening distance. In this exemplary arrangement, the upper and lower concentrate vane distances are less than the concentrate pump opening distance. Of course, it should be understood that this configuration is exemplary, and other embodiments of other methods may be used.

In the exemplary single-use configuration 314, the upper and lower

concentrate compartment vanes

344, 340, and the upper and lower

centrifuge compartment vanes

326, 328 operate to stably and radially position the annular air/liquid interface 348 in the centrifuge pump chamber 330 and the air/liquid interface 350 in the concentrate pump chamber 332. As shown in fig. 28, the air/liquid interface 348 is positioned radially midway along the radial length of the centrifuge chamber vanes. This is radially inward from the centrifuge pump opening 330. The radially extending centrifuge chamber vanes operate to provide a centrifugal pumping force that maintains the annular air/liquid interface 348 at radial positions above and below the centrifuge pump, which are disposed radially inward of the centrifuge pump opening 330. In this exemplary arrangement, the vanes further help stabilize the air/liquid interface so that it remains in a coaxial circular configuration both above and below the centrifuge pump. Furthermore, in an exemplary arrangement, the radial position of the interface relative to the axis of rotation may be controlled as discussed later such that the centrifuge pump opening 330 remains in the liquid centrifuge at all times and is not exposed to air.

The upper concentrate compartment blades 344 and the lower concentrate compartment blades 340 operate in a similar manner as the centrifuge compartment blades. The concentrate chamber vanes maintain a circular air/liquid interface 350 in the concentrate pump chamber 332 at a radial distance inside the substantially annular concentrate pump opening 346. This configuration ensures that the concentrate pump opening is always exposed to concentrate rather than air. It should also be understood that although in the illustrated embodiment the centrifuge centripetal pump and the concentrate centripetal pump are of substantially the same size, other arrangements of the centripetal pumps may be of different sizes. In this case, the radial distance from the rotational axis, through which the centrifuge chamber vanes and the concentrate chamber vanes extend, may be different. Furthermore, the radial position of the air/liquid interface in the centrifuge pump chamber and the concentrate pump chamber with respect to the axis of rotation may be different. A variety of different blade configurations and arrangements may be used depending on the particular relationship between the components making up the single-use set and the particular materials being handled via the single-use configuration.

Figure 30 shows an upper portion of another alternative single-use construction 352. Single-use configuration 352 is similar to single-use configuration 304, except as discussed otherwise. The single-use arrangement 352 includes an air tube 354 that extends in coaxially surrounding relation to the concentrate discharge tube 220. The air tube 354 communicates with an opening 356 in the single-use configuration. The opening 356 extends from the interior of the air tube to above the concentrate radial pump 216 in the concentrate pump chamber 332. In this exemplary arrangement, the seal 236, as schematically shown, operatively engages the air tube 354 to maintain an air tight engagement with the air tube and the concentrate discharge tube, the centrate discharge tube and the feed tube. As can be appreciated, the air tube may be used to selectively maintain the air pressure level in the air pocket within the single-use configuration. This arrangement may be used in conjunction with the system described previously, or in other systems, where externally supplied pressurized air is used to isolate the seals of the centrifuge structure from the material being processed and to maintain the air/liquid interface in a desired location. Of course, it should be understood that this configuration is exemplary and other embodiments of other methods may be used.

Fig. 31 schematically illustrates a system 358 that may be used to continuously separate a cell suspension into a substantially cell-free centrate and concentrate. The system 358 is similar to the system 170 previously discussed, except as otherwise noted herein. In this exemplary arrangement, the system 358 operates using a single-use configuration similar to the single-use configuration 352. The controller 274 of the system 358 operates to control the position of the air/liquid interface within the single-use configuration to ensure that the interface is maintained radially inward from each of the centrifuge pump opening and the concentrate pump opening relative to the axis of rotation.

In this exemplary arrangement, a flow back pressure regulator 360 is fluidly connected to the centrate discharge line 262. In this exemplary arrangement, the flow back pressure regulator 360 is intermediate the flow of the centrate discharge tube 212 and the centrate pump 266. The exemplary system 358 includes a source of pressurized air schematically represented at 362. A source of pressurized air 362 is connected to a pilot pressure control valve 364. The control valve is operatively connected to a controller 274. The signal from the controller 274 causes a selectively variable pressure in the pilot line 366. Pilot line 366 is fluidly connected to back pressure regulator 360. The pressure applied in the pilot line 366 by the pilot pressure control valve 264 is operable to control the centrifugal flow and, thus, the centrifugal flow back pressure applied by the flow back pressure regulator 360.

In the exemplary arrangement, pressure control valve 368 is in fluid communication with pressurized air source 362. Control valve 368 is also operatively connected to controller 274. In this exemplary arrangement, the control valve 368 is controlled to selectively apply precise pressure to the air tube 354 and the air pocket within the upper portion of the single-use construction 352.

In this exemplary arrangement, the controller 274 operates in accordance with stored executable instructions to control the operation of the system 358 in a manner similar to that previously discussed in connection with the system 170. Further, in the exemplary arrangement, controller 274 operates to control pilot pressure valve 364 to vary the back pressure applied by back pressure regulator 360 to centrifuge discharge tube 212. Controller 274 also operates to control valve 368. The controller operates to maintain and selectively vary the pressure applied in the air pocket at the top of the interior of the single-use construction. The controller operates according to its programming to vary the back pressure and/or cavitation pressure of the centrifuge stream to maintain the air/liquid interface of the cavitation at a radial distance from the axis of rotation that is inward from the centrifuge pump opening 330 and the concentrate pump opening 346. This pressure change in both the centrifuge flow back pressure and the cavitation pressure, in combination with the action of the centrifuge chamber vanes and the concentrate chamber vanes in this exemplary embodiment, maintains the stability and radial outward extent of the air/liquid interface to ensure that air induction is minimized in the centrifuge and concentrate output from the single use configuration. In addition, the ability to selectively vary the backpressure and flow rate of the centrate may affect the level of cells and the corresponding detected optical density of the discharged concentrate. Thus, the controller may operate in accordance with its programming to selectively vary the concentrate flow rate, the centrifuge back pressure and flow rate, the internal air pocket pressure, the feed rate of the cell suspension into the single use configuration, and possibly other operating variables of the centrifugation process to maintain the centrifuge and concentrate properties within set limits and/or ranges stored in at least one data storage associated with the controller. Furthermore, this exemplary arrangement enables different types of materials to be separated and operated at different flow rates while maintaining reliable control of the separation process. Of course, while it should be understood that control of the position of the air/liquid interface is described in connection with the features of system 170, such control may be used in other types of systems including other or different types of processing elements.

Accordingly, the new centrifuge system and method of this exemplary embodiment achieves at least some of the above objectives, eliminates difficulties encountered when using existing devices and systems, solves problems, and achieves the desired results described herein.

In the foregoing description, certain terms have been used for brevity, clarity, and understanding, however, no unnecessary limitations are to be implied therefrom because such terms are used for descriptive purposes and are intended to be broadly construed. Furthermore, the descriptions and illustrations herein are by way of example and the invention is not limited to the exact details shown and described.

In the following claims, any feature described as a means for performing a function shall be construed as encompassing any means known to those skilled in the art to be capable of performing the recited function, and shall not be limited to the structures shown herein or their pure equivalents.

Having described the features, discoveries and principles of new and useful features, the manner of constructing, utilizing and operating the same, and the advantages and useful results attained, the new and useful structures, devices, elements, arrangements, components, combinations, systems, apparatuses, operations and relationships thereof are set forth in the appended claims.

Claims

1. An apparatus, comprising:

a single-use configuration configured for use in a centrifuge system comprising a multi-use rigid rotating centrifuge bowl, wherein the single-use configuration is configured to be positioned in the rigid bowl and separate cells in a cell culture batch into a cell concentrate and a cell centrifuge within an interior region of the single-use configuration,

the single-use configuration in the operative position includes:

the upper part of the disc shape is provided with a disc shape,

the lower part of the lower part is provided with a plurality of grooves,

a hollow, at least semi-rigid, cylindrical core intermediate said upper and lower portions,

a separation chamber in radially surrounding relation to the core,

a flexible outer wall, wherein the outer wall

Extends in fluid-tight relationship with the disc-shaped upper portion and delimits the separation chamber, extends in surrounding relationship with the core, and

having an internal truncated conical shape with a smaller internal diameter adjacent to the lower portion,

a feeding pipe which extends vertically is arranged on the feeding pipe,

a centrifuge discharge tube extending vertically,

a vertically extending concentrate discharge tube,

wherein the disc-shaped upper portion and the outer wall are rotatable about a vertical axis within the drum,

wherein during rotation of the drum, the disc-shaped upper portion and the outer wall rotate relative to each of the feed pipe, the centrate discharge pipe, and the concentrate discharge pipe,

a centrifuge centripetal pump, wherein the centrifuge centripetal pump is axially aligned with the core, is coaxially disposed about the feed tube, and is in fluid communication with the centrifuge discharge tube,

wherein the centrifuge centripetal pump is positioned in the interior region in a centrifuge pump chamber, wherein the centrifuge pump chamber is in fluid communication with the separation chamber,

wherein during rotation of the drum, the disc-shaped upper portion and the outer wall rotate relative to the centrifuge centripetal pump,

a concentrate centrifugal pump, wherein the concentrate centrifugal pump is axially aligned with the core, is coaxially disposed about the feed tube, is positioned vertically above the centrifuge centrifugal pump, and is in fluid communication with the concentrate discharge tube,

wherein the concentrate centripetal pump is positioned in the interior region in a concentrate pump chamber,

wherein the concentrate pump chamber is in fluid communication with the separation chamber, and wherein, during rotation of the drum, the disc-shaped upper portion and the outer wall rotate relative to the concentrate centrifugal pump.

2. The apparatus as set forth in claim 1, wherein,

wherein the outer wall includes a textured outer surface that enables air to escape from between the outer wall and the drum.

3. The apparatus as set forth in claim 2, wherein,

wherein the textured outer surface comprises a pattern of outwardly extending protrusions with intermediate recesses between the protrusions,

wherein the pattern of protrusions and recesses enables air to escape from between the outer wall and the drum.

4. The apparatus as set forth in claim 2, wherein,

wherein the disc-shaped upper portion within the interior region is substantially rigid,

wherein the disc shaped upper portion is configured to releasably operatively engage the rigid drum.

5. The apparatus as set forth in claim 4, wherein,

wherein the lower portion within the interior region comprises

A plurality of angularly spaced radially extending accelerator vanes, wherein fluid passages extend between the vanes.

6. The apparatus as set forth in claim 5, wherein,

wherein the lower portion of the disc shape is fixedly operatively connected with the core.

7. The apparatus as set forth in claim 6, wherein,

wherein said flexible outer wall extends intermediate said disc-shaped lower portion and said rigid drum.

8. The apparatus as set forth in claim 7, wherein,

wherein the radially extending accelerator blades extend upwardly from the lower portion of the disk shape.

9. The apparatus as set forth in claim 7, wherein,

wherein the radially extending accelerator blades extend downwardly from the lower portion of the disk shape.

10. The apparatus as set forth in claim 7, wherein,

wherein the single-use configuration enables the centrifuge system to operate with at least one of a continuous or semi-continuous discharge of cell centrate and cell centrate.

11. The apparatus as set forth in claim 7, wherein,

wherein the outer wall has a larger inner diameter at a vertical height corresponding to a top of the core than at a vertical height corresponding to a lower portion of the core.

12. The apparatus as set forth in claim 11, wherein,

wherein the concentrate pump chamber is in fluid communication with the separation chamber through a substantially annular concentrate opening,

wherein the centrifuge pump chamber is in fluid communication with the separation chamber through a substantially annular centrifuge opening,

wherein the substantially annular concentrate opening and the centrifuge opening are coaxial and the substantially annular concentrate opening is disposed radially outward from the substantially annular centrifuge opening.

13. The apparatus of claim 12, further comprising

An external concentrate pump, wherein the external concentrate pump is external to the single-use configuration and fluidly connected to the concentrate discharge tube,

a concentrate optical density sensor, wherein the concentrate optical density sensor is in operative connection with an interior of a concentrate discharge line, wherein the concentrate discharge line is in operative connection with the concentrate discharge tube and the external concentrate pump,

an external centrifuge pump, wherein the external centrifuge pump is external to the single use configuration and fluidly connected to the centrifuge discharge tube,

a centrifuge optical density sensor, wherein the centrifuge optical density sensor is in operative connection with an interior of a centrifuge discharge line, wherein the centrifuge discharge line is in operative connection with the centrifuge discharge tube and the external centrifuge pump,

a controller, wherein the controller is in operative connection with the external centrifuge pump, the external concentrate pump, the centrifuge optical density sensor, and the concentrate optical density sensor, wherein the controller is operative to control at least one of the external concentrate pump and the external centrifuge pump at least partially in response to the concentrate optical density sensor and the centrifuge optical density sensor.

14. The apparatus of claim 13, further comprising

At least one annular gas-tight seal which is,

wherein at least one said seal extends in operative sealing relation between said disc-shaped upper portion and at least one annular outer wall extending outwardly from at least one of said feed tube, said centrifuge discharge tube and said concentrate discharge tube,

wherein at least one said seal operates to maintain an air pocket within said interior region, wherein air in said air pocket isolates at least one said seal from a cell culture batch being processed while maintaining said cell culture batch in fluid connection with each of said centrifuge pump and said concentrate pump.

15. The apparatus as set forth in claim 14, wherein,

wherein the centrifuge chamber is vertically delimited by:

a circular axially centered lower centrifuge is placed against the pumping chamber surface,

wherein the lower centrifuge chamber surface comprises an axially centered lower centrifuge chamber opening, wherein the feed tube extends through the lower centrifuge chamber opening,

an axially centered circular upper centrifuge is positioned against the pumping chamber surface,

wherein the upper centrifuge chamber surface comprises an axially centered upper centrifuge chamber opening,

wherein the feed pipe and the vertically extending centrifuge discharge pipe extend through the upper centrifuge chamber opening,

wherein the centrifuge pump comprises a substantially annular centrifuge pump opening, wherein the centrifuge from the centrifuge pump chamber enters the centrifuge pump through the substantially annular centrifuge pump opening,

wherein the substantially annular centrifuge pump opening is radially arranged at a centrifuge pump opening distance from the axis,

wherein the lower centrifuge chamber surface comprises

A plurality of angularly spaced, radially upwardly extending lower centrifuge chamber vanes, wherein said lower centrifuge chamber vanes extend radially outwardly from said lower centrifuge chamber opening a lower centrifuge vane distance, wherein said lower centrifuge vane distance is less than said centrifuge pump opening distance,

wherein the upper centrifuge chamber surface comprises a plurality of angularly spaced radially downwardly extending upper centrifuge chamber vanes,

wherein the upper centrifuge chamber vanes extend radially outward an upper centrifuge vane distance from the upper centrifuge to a centrifuge chamber opening, wherein the upper centrifuge vane distance is less than the centrifuge pump opening distance.

16. The apparatus of claim 15

Wherein the concentrate centripetal chamber is vertically defined by:

a circular axially centered lower concentrate is applied to the pumping chamber surface,

wherein the lower concentrate centripetal chamber surface comprises an axially centered lower concentrate centripetal chamber opening, wherein the feed pipe and the vertically extending centrifuge discharge pipe extend through the lower concentrate centripetal chamber opening,

an axially centered circular upper concentrate is pumped to the pumping chamber surface,

wherein the upper concentrate pumping chamber surface comprises an axially centered upper concentrate pumping chamber opening,

wherein the feed pipe, the vertically extending centrifuge discharge pipe and the vertically extending concentrate discharge pipe extend through the upper concentrate-centripetal chamber opening,

wherein the concentrate pump comprises a substantially annular concentrate pump opening, wherein concentrate from the concentrate pump chamber enters the concentrate pump through the substantially annular concentrate pump opening,

wherein the substantially annular concentrate pump opening is radially arranged at a concentrate pump opening distance from the axis,

wherein the lower concentrate comprises

A plurality of angularly spaced radially upwardly extending lower concentrate compartment vanes,

wherein the lower concentrate chamber vanes extend radially outward a lower concentrate vane distance from the lower concentrate pumping chamber opening, wherein the lower concentrate vane distance is less than the concentrate pumping chamber opening distance,

wherein the upper concentrate comprises

A plurality of angularly spaced radially downwardly extending upper concentrate compartment vanes,

wherein the upper concentrate chamber vanes extend radially outward an upper concentrate vane distance from the upper concentrate toward the pumping chamber opening, wherein the upper concentrate vane distance is less than the concentrate pump opening distance.

17. The apparatus of claim 16, further comprising:

a source of pressurized air, wherein the source of pressurized air is external to the single-use construction,

wherein the source of pressurized air is in operative connection with the air pocket,

wherein the source of pressurized air operates to maintain the air pocket disposed radially inward of each of the substantially annular centrifuge pump opening and the substantially annular concentrate pump opening.

18. The apparatus of claim 17, further comprising:

a back-pressure regulator of the flow is provided,

wherein the flow back pressure regulator is external to the single use configuration and fluidly connected to the centrate discharge tube,

wherein the flow back pressure regulator operates to selectively apply back pressure to a centrifuge stream from the single-use configuration,

wherein the flow back pressure regulator is in operative connection with the controller,

wherein the controller is operative to control the flow back pressure regulator to maintain the pockets radially inward from each of the substantially annular centrifuge pump opening and the substantially annular concentrate pump opening.

19. The apparatus of claim 18, further comprising:

a continuous annular dam of concentrate is formed,

wherein the annular concentrate dam

Extending in the separation chamber and being provided with a plurality of openings,

extends downwardly below said substantially annular concentrate opening, and

is arranged radially inside said substantially annular concentrate opening.

20. The apparatus of claim 19, further comprising:

a continuous annular centrifuge dam is provided,

wherein the annular centrifuge dam

extends down below said substantially annular centrifuge opening, and

arranged radially outside said substantially annular centrifuge opening,

wherein an annular upwardly extending recess extends radially in the separation chamber between the annular concentrate dam and the annular centrifuge dam.

21. The apparatus as set forth in claim 1, wherein,

wherein the outer wall comprises a pattern of outwardly extending protrusions with intermediate recesses between the protrusions,

22. The apparatus as set forth in claim 1, wherein,

23. The apparatus as set forth in claim 1, wherein,

wherein the lower portion within the interior region comprises

24. The apparatus as set forth in claim 1, wherein,

wherein the lower portion within the interior region comprises

A substantially rigid disc-shaped lower portion of the container,

wherein the disc-shaped lower portion is fixedly operatively connected with the core.

25. The apparatus as set forth in claim 1, wherein,

wherein the lower portion within the interior region comprises

A substantially rigid disc-shaped lower portion of the container,

wherein the disc-shaped lower portion is fixedly operatively connected with the core,

26. The apparatus as set forth in claim 1, wherein,

wherein the lower portion within the interior region comprises

A substantially rigid disc-shaped lower portion of the container,

wherein the disk-shaped lower portion includes a plurality of angularly spaced radially extending accelerator blades, wherein the blades extend upwardly from the disk-shaped lower portion and fluid passages extend radially between the blades.

27. The apparatus as set forth in claim 1, wherein,

wherein the lower portion in the inner region comprises

A substantially rigid disc-shaped lower portion of the container,

wherein the disk-shaped lower portion includes a plurality of angularly spaced radially extending accelerator blades, wherein the blades extend downwardly from the disk-shaped lower portion and fluid passages extend between the blades.

28. The apparatus as set forth in claim 1, wherein,

29. The apparatus as set forth in claim 1, wherein,

30. The apparatus as set forth in claim 1, wherein,

31. The apparatus of claim 1, further comprising

a controller, wherein the controller is in operative connection with the external concentrate pump and the concentrate optical density sensor, wherein the controller is operative to control the external concentrate pump at least partially in response to the concentrate optical density sensor.

32. The apparatus of claim 1, further comprising

a controller, wherein the controller is in operative connection with the external centrate pump and the centrate optical density sensor, wherein the controller is operative to control the external centrate pump at least partially in response to the centrate optical density sensor.

33. The apparatus of claim 1, further comprising

34. The apparatus of claim 1, further comprising

At least one annular gas-tight seal which is,

wherein at least one said seal operatively extends in sealing relation between said disc-shaped upper portion and at least one annular outer wall extending outwardly from at least one of said feed tube, said centrifuge discharge tube and said concentrate discharge tube,

35. The apparatus as set forth in claim 1, wherein,

wherein the centrifuge chamber is vertically delimited by:

wherein the lower centrifuge chamber surface comprises

wherein the upper centrifuge chamber surface comprises

A plurality of angularly spaced radially downwardly extending upper centrifuge chamber vanes,

36. The apparatus as set forth in claim 1, wherein,

wherein the concentrate centripetal chamber is vertically defined by:

wherein the lower concentrate comprises

A plurality of angularly spaced radially upwardly extending lower concentrate compartment vanes, wherein the lower concentrate compartment vanes extend radially outwardly from the lower concentrate centripetal chamber opening by a lower concentrate vane distance, wherein the lower concentrate vane distance is less than the concentrate pump opening distance,

wherein the upper concentrate comprises

37. The apparatus as set forth in claim 1, wherein,

wherein the substantially annular concentrate opening and the centrifuge opening are coaxial and the substantially annular concentrate opening is disposed radially outward from the substantially annular centrifuge opening,

a continuous annular centrifuge dam is provided,

wherein the annular centrifuge dam

extends down below said substantially annular centrifuge opening, and

is arranged radially outside said substantially annular centrifuge opening.

38. The apparatus as set forth in claim 1, wherein,

a continuous annular centrifuge dam is provided,

wherein the annular centrifuge dam

extends down below said substantially annular centrifuge opening, and

arranged radially outside said substantially annular centrifuge opening,

a continuous annular dam of concentrate is formed,

wherein the annular concentrate dam

extends downwardly below said substantially annular concentrate opening, and

is arranged radially inside said substantially annular concentrate opening.

39. The apparatus as set forth in claim 1, wherein,

a continuous annular centrifuge dam is provided,

wherein the annular centrifuge dam

extends down below said substantially annular centrifuge opening, and

arranged radially outside said substantially annular centrifuge opening,

a continuous annular dam of concentrate is formed,

wherein the annular concentrate dam

extends downwardly below said substantially annular concentrate opening, and

arranged radially inside said substantially annular concentrate opening,

40. The apparatus of claim 1, further comprising:

at least one annular gas-tight seal which is,

wherein at least one said seal operatively extends in sealing relation between said disc-shaped upper portion and at least one annular outer wall extending radially outwardly from at least one of said feed tube, said centrifuge discharge tube and said concentrate discharge tube,

wherein the centrifuge pump comprises a substantially annular centrifuge pump opening, wherein the centrifuge passes from the centrifuge pump chamber through the substantially annular centrifuge pump opening,

wherein the concentrate pump comprises a substantially annular concentrate pump opening, wherein concentrate passes from the concentrate pump chamber through the substantially annular concentrate pump opening,

wherein at least one of the seals operates to maintain an air pocket within the interior region, wherein the source of pressurized air is fluidly connected with the air pocket,

wherein air in the air pocket isolates at least one of the seals from a cell culture batch being processed, and wherein the air pocket is positioned radially inside each of the substantially annular centrifuge pump opening and the substantially annular concentrate pump opening.

41. The apparatus of claim 1, further comprising:

at least one annular gas-tight seal which is,

wherein at least one said seal operates to maintain an air pocket within said interior region,

wherein air in the air pocket isolates at least one of the seals from a cell culture batch being processed,

a flow back-pressure regulator fluidly connected to the centrate discharge conduit, wherein the flow back-pressure regulator is selectively operable to back-pressure the centrate stream,

a controller, wherein the controller is in operative connection with the flow back pressure regulator, wherein the controller is operative to maintain the air pocket positioned radially inside both the substantially annular centrifuge pump opening and the substantially annular concentrate pump opening.

42. An apparatus, comprising:

a single-use configuration configured for use in a centrifuge system comprising a multi-use rigid rotating centrifuge bowl, wherein the single-use configuration is configured to be releasably positioned in the rigid bowl and separate cells in a cell culture batch into a cell concentrate and a cell centrifuge within an interior region of the single-use configuration,

the single-use construction includes:

a first disc-shaped portion located at a first axial end,

a second axial end disposed axially from the first axial end,

a hollow cylindrical core coaxially disposed with the first disk-shaped portion and axially intermediate the first disk-shaped portion and the second axial end,

a flexible outer wall, wherein the outer wall

In fluid-tight relationship with said first disc-shaped portion,

extending in surrounding relation to at least a portion of the core,

defining a separation chamber extending radially between the core and the outer wall,

is tapered such that a radius of the outer wall is greater axially adjacent the first disc-shaped portion than axially adjacent the second axial end,

an axially extending feed tube, wherein the feed tube includes an inlet within the interior region,

an axially extending centrate discharge tube coaxial with and radially outward from said feed tube,

an axially extending concentrate discharge tube coaxial with and radially outward from the centrifuge discharge tube,

a centrifuge centripetal pump, wherein the centrifuge centripetal pump is coaxially disposed about the feed tube and is in fluid communication with the centrifuge discharge tube,

wherein during rotation of the single-use configuration and the centrifuge bowl, the first disk-shaped portion and the outer wall rotate relative to the centrifuge bowl,

a concentrate centrifugal pump, wherein the concentrate centrifugal pump is coaxially disposed about the feed tube, is in fluid communication with the concentrate discharge tube, and is axially positioned intermediate the centrifuge centrifugal pump and the first disk portion,

wherein the concentrate pump chamber is in fluid communication with the separation chamber, and wherein the first disk shaped portion and the outer wall rotate relative to the concentrate radial pump during rotation of the single use configuration and the centrifuge bowl.

43. In accordance with the apparatus set forth in claim 42,

wherein the flexible outer wall comprises a textured outer surface,

wherein the textured outer surface is configured to enable air to be released from between the outer wall and the drum.

44. In accordance with the apparatus set forth in claim 42,

wherein the outer wall defines the interior region of the single-use construction at the second axial end.

45. The apparatus according to claim 42, further comprising a second disc portion,

wherein the second disc portion

Is axially disposed within the interior region from the first disc-shaped portion,

operatively supportingly connected with the core and including a plurality of angularly spaced radially extending vanes with fluid passages extending between the vanes.

46. In accordance with the apparatus set forth in claim 42,

wherein the outer wall defines the interior region of the single-use construction at the second axial end,

wherein the single-use construction further comprises a second disc portion within the interior region, wherein the second disc portion

is operatively connected in supporting relation to said core,

comprising a plurality of angularly spaced radially extending vanes, wherein fluid passages extend radially between the vanes,

wherein the vanes extend axially from the second disk portion toward the second axial end.

47. The apparatus of claim 42, further comprising:

at least one annular gas-tight seal which is,

wherein at least one said seal extends in operative sealing relation between said first disc-shaped portion and at least one annular outer wall extending outwardly from at least one of said concentrate discharge tube, said centrifuge discharge tube and said feed tube,

wherein, during cell culture batch processing, at least one said seal operates to maintain an air pocket within said interior region, wherein air in said air pocket isolates at least one said seal from said cell culture batch while maintaining said cell culture batch in fluid connection with each of said centrifuge pump and said concentrate pump.

48. In accordance with the apparatus set forth in claim 42,

49. The apparatus of claim 42, further comprising