CN111999348A - Detection method or kit - Google Patents
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- CN111999348A CN111999348A CN201910485511.XA CN201910485511A CN111999348A CN 111999348 A CN111999348 A CN 111999348A CN 201910485511 A CN201910485511 A CN 201910485511A CN 111999348 A CN111999348 A CN 111999348A
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Abstract
The invention designs a method or a kit for detecting 2-imino-1-methylimidazoline-4-one, belonging to the technical field of medical inspection. The components of the kit comprise a compound with the following general formula A. The detection method or the kit can effectively eliminate or reduce the interference of some common interference substances existing in the sample on the detection result, and improve the reliability of the detection result.
Description
Technical Field
The invention relates to the technical field of medical examination. In particular, the present invention relates to a method or a kit for detecting 2-imino-1-methylimidazolin-4-one.
Background
2-imino-1-methylimidazolin-4-one is an important index for judging renal function, and clinically, there are various detection methods for 2-imino-1-methylimidazolin-4-one, such as picric acid method, oxidase method, electrochemical method, and dehydrogenase method.
The method which is most clinically applied at present is an oxidase method, and the reaction principle of the method comprises the following steps: the content of 2-imino-1-methylimidazolin-4-one was calculated by measuring the amount of hydrogen peroxide generated by converting 2-imino-1-methylimidazolin-4-one into N-methylglycine, converting N-methylglycine into N-methylglycine, and then oxidizing N-methylglycine to produce hydrogen peroxide.
The oxidase method has the advantage of economy and convenience, but the intermediate in the detection step of the oxidase method is hydrogen peroxide, which is unstable and is easily interfered by other substances existing in the sample. One common example is: after a patient takes or injects a medicine containing 2, 5-dihydroxybenzenesulfonate or dopa, the 2, 5-dihydroxybenzenesulfonic acid or salt thereof or dopa medicine existing in the blood of the patient can cause the serious low determination result of 2-imino-1-methylimidazolin-4-one, thereby causing misjudgment of the disease condition of the patient and causing serious consequences.
In addition, the experiment also shows that a plurality of compounds, such as tocopherol, gentisic acid, gallic acid, catechin, and MEDOPA, can also interfere the detection of 2-imino-1-methylimidazoline-4-ketone, and cause misjudgment of the result.
Therefore, there is a need in the art for a suitable technique that can eliminate or reduce interference of 2, 5-dihydroxybenzenesulfonate or dopas in a sample and ensure the reliability of the detection result of 2-imino-1-methylimidazolin-4-one.
Disclosure of Invention
The invention provides a detection method or a kit, which is used for detecting the content of 2-imino-1-methylimidazoline-4-ketone in a sample and comprises the following steps: a step of contacting a test sample with a compound represented by the general formula A;
and a kit comprising a compound of formula a as described above. In addition, the detection method or the kit provided by the invention also comprises a step of converting 2-imino-1-methylimidazoline-4-ketone into hydrogen peroxide; and a step of obtaining the content of 2-imino-1-methylimidazolin-4-one by detecting hydrogen peroxide.
In the art, the method of converting 2-imino-1-methylimidazolin-4-one into hydrogen peroxide by using several tool enzyme coupling is well known, and there are many reports on the discussion and combination of the manufacturer, amount, placement position in the kit, required buffer system, surfactant, preservative, etc., of the tool enzyme therein, and not much description is given here.
Electrochemical electrode methods are used for detecting hydrogen peroxide, but the most widely used method is the one generally named Trinder's reaction. In the Trinder's reaction, under the action of peroxidase, hydrogen peroxide and 4-aminoantipyrine or an analogue thereof and a chromogen can be reacted to generate a colored compound. Typical chromogens include phenol, and novel chromogens of the types abbreviated as ADOS, ADPS, ALPS, DAOS, HDAOS, MADB, MAOS, TODB, TOOS, TOPS, and the like, which vary in sensitivity and stability and are reported in a number of published art and not discussed herein in any significant amount.
In the compounds of formula a as described above, wherein R1, R2, R3, R4 are groups which may be attached to the carbon chain, most commonly hydrocarbyl or modified hydrocarbyl groups, and hydrogen atoms, which may be the same or different; r5 is a group that can be attached to a carbon chain.
In a preferred embodiment, R1, R2, R3 and R4 are all methyl groups, but it is understood that R1, R2, R3 and R4 can be substituted with the same or different substituents, such as ethyl groups, or propyl groups, aliphatic hydrocarbon chains, aromatic hydrocarbons, substituted hydrocarbons, such as halogenated hydrocarbons, fatty acid chains, ketone-containing chains, and the like, and combinations thereof, including combinations with methyl groups or hydrogen atoms, as is known in the art. This is a common technique of organic chemistry and is not described in great detail here.
R5 is also a group bonded to a carbon atom, for example, a hydrogen atom, a hydroxyl group, an oxygen atom, an amino group, a carboxyl chain, a mercapto group, a halogen atom, an alkyl group, an aralkyl group, a hydrocarbon group, an aryl group, a substituted oxy group, a substituted amino group, a substituted hydrocarbon group, a substituted thio group, a cyano group, an isothiocyanate group or the like. Among them, hydrogen atom, hydroxyl group, cyano group, carboxyl group, keto group, methoxy group, acetylamino group, cyano group, isothiocyanato group, methacryloyloxy group, 2-chloroacetamido group and the like are more common.
When 2-imino-1-methylimidazolin-4-one is converted to hydrogen peroxide, since hydrogen peroxide is a very active substance, it reacts with many substances, particularly reducing substances. The aforementioned 2, 5-dihydroxybenzenesulfonic acid or salts thereof and methyldopa may be the result of a destructive or competitive reaction with hydrogen peroxide, which leads to a pseudo-low measured value for 2-imino-1-methylimidazolin-4-one.
In the invention, through a large number of screening experiments, the substance shown in the general formula A has a very good effect when being contacted with a sample, and the interference of 2, 5-dihydroxybenzenesulfonic acid and methyldopa on detection can be effectively eliminated or reduced. For example, for a sample containing a very high concentration of calcium 2, 5-dihydroxybenzenesulfonate, the method described by the invention can reduce the interference degree to be within 15% of the relative deviation, and even can reduce the interference degree to be within 8% of the relative deviation under the conventional dosage concentration, thereby achieving the effect of eliminating the interference.
In this study, it was found that various salts of 2, 5-dihydroxybenzenesulfonic acid, such as calcium salts, diethylamine salts, various dopa-based substances, including carbidopa, levodopa, and medopa, interfere with the detection of 2-imino-1-methylimidazolin-4-one using conventional techniques. Further research shows that the substances have common structural characteristics and are connected with hydroxyl on a benzene ring. In view of this finding, we tested more compounds having a hydroxyl group attached to the benzene ring, such as tocopherol, sulfophenol, gentisic acid, gallic acid, catechin, resveratrol, dihydroxynaphthalene, and found that the results of the detection of 2-imino-1-methylimidazolin-4-one by the conventional oxidase method interfered with.
In a larger number of experiments, it has been found that when the benzene ring of the above-mentioned compounds is replaced by an aromatic heterocycle, for example 2, 6-dihydroxypyridine-4-carboxylic acid, 5-hydroxyindole; 3, 4-dihydroxyfuran-2, 5-dicarboxylic acid, 2, 5-dihydroxy-3-thiopheneethanesulfonic acid, 5, 6-dihydroxypyrimidine-4-carboxylic acid, and the like, also interfere with the results of detection of 2-imino-1-methylimidazolin-4-one using conventional techniques.
Combining the above research results, a rule can be found: that is, when a hydroxyl group is bonded to a benzene ring, a naphthalene ring, another aromatic ring or an aromatic heterocyclic ring, particularly when a plurality of hydroxyl groups are bonded, interference with the detection of 2-imino-1-methylimidazolin-4-one by conventional techniques may occur. Of course, numerous organic compounds that meet the above characteristics can be cited based on the knowledge of well-known organic chemistry.
In the present study, it was surprisingly found through experimental verification that the interference of the compounds of formula a can be effectively reduced by contacting the sample with the compounds of formula a according to the method of the present invention.
Therefore, the method provided by the invention, which adopts the compound of the general formula A, can not only eliminate or reduce the interference of 2, 5-dihydroxybenzenesulfonic acid or salt thereof or dopa compound in the sample on the measurement result. It is also possible to eliminate or reduce interference of the measurement result with a compound containing a hydroxyl group attached to an aromatic ring or an aromatic heterocyclic ring in a sample.
Based on the above studies, we have further conducted further studies, and further found that some of the m-diketones, such as 1, 3-cyclohexanedione, mesotrione, also interfere with the detection of 2-imino-1-methylimidazolin-4-one. Surprisingly, it has also been found that the compounds of formula A can be used to effectively eliminate or reduce interference from such substances when they are contacted with a sample using the methods of the present invention. It is assumed that the mechanism of the reaction is that the structure of the m-diketone may be isomerized to produce an enol structure, and a hydroxyl group connected to the double bond appears, and the hydroxyl group has a certain reducibility, thereby destroying the detection of hydrogen peroxide. The formula a of the present invention unexpectedly provides the ability to eliminate or mitigate such interference.
Based on the method provided by the invention, a kit for detecting 2-imino-1-methylimidazoline-4-ketone is prepared, the performance of the kit is verified, and the capability of resisting some conventional interfering substances is tested. In a validation experiment, it is very surprising to find that the kit adopting the method described by the invention also has the capacity of resisting the interference of other various substances, including the capacity of resisting the interference of hemoglobin, the capacity of resisting the interference of reduced glutathione and the capacity of resisting the interference of ascorbic acid.
Hemoglobin is an iron-containing porphyrin protein, and also commonly used proteins containing iron porphyrin protein include myoglobin, cytochrome C, and the like. Iron porphyrins are oxidative and may be capable of spontaneously generating reactive oxygen species, thereby interfering with the detection of hydrogen peroxide. Therefore, the Trinder's reaction can be initiated without hydrogen peroxide to generate a colored compound, thereby interfering with the detection result. It is presumed that the substance represented by the above general formula A may quench active oxygen and thus has an effect of inhibiting interference of iron-containing porphyrin protein.
The reducing property of the sulfhydryl of the compound containing active sulfhydryl, such as common reduced glutathione, cysteine and the like, can destroy hydrogen peroxide, so the compound can interfere the detection of 2-imino-1-methylimidazoline-4-ketone by the conventional technology, but the effect of the sulfhydryl compound on the detection result can be effectively reduced by contacting the compound with the general formula A with a sample by adopting the method provided by the invention.
Ascorbic acid is a very common interfering substance in Trinder's reaction, and the interference elimination of ascorbic acid is generally completed by adopting a cheap and mature ascorbic acid oxidase method. However, the method provided by the invention also has the effect of eliminating the interference of the ascorbic acid, and provides an additional solution.
Therefore, in the invention, the method or the kit described in the invention realizes the purpose of solving the problem of interference of 2, 5-dihydroxybenzenesulfonic acid or salts thereof, dopa and other compounds with hydroxyl groups connected to aromatic rings, which is not solved by a mature and effective means at present, and has the capability of additionally interfering with substances such as an anti-sulfhydryl compound, iron-containing porphyrin protein, ascorbic acid and the like, which is also a significant difference and a remarkable improvement of the invention from the prior art.
In the present invention, the whole detection process comprises a step of contacting the sample with the substance of the above general formula A, and a step of converting 2-imino-1-methylimidazolin-4-one into hydrogen peroxide, and further comprises a step of detecting hydrogen peroxide.
The step of converting 2-imino-1-methylimidazolin-4-one to hydrogen peroxide is a conventional technique in the art, most commonly using an enzyme to gradually convert 2-imino-1-methylimidazolin-4-one to N-methylguanidinoacetic acid and N-methylglycine, which is then oxidized to produce hydrogen peroxide. Details concerning these steps, including the amount of enzyme, the concentration of buffers, surfactants, preservatives, etc., and the placement of the components of each part of the kit, are well known in the art and are well documented and reported. If Tinder's reaction is used to detect hydrogen peroxide, the type and amount of chromogen, the amount of 4-aminoantipyrine or the like, the amount of peroxidase, etc. used therein also belong to the art disclosed in this field, and there are many documents and reports. If the electrode method is used for detecting the hydrogen peroxide, the used electrode, signal and data conversion also belong to the well-known technology in the industry. Nor will it be discussed more here.
The three detection steps are not necessarily carried out separately, and as a person skilled in the art, the three detection steps can be carried out respectively or simultaneously by reasonably designing and controlling the reaction speed of the three steps.
For example, if the amount of the substance of formula A is too large during the reaction, the interference elimination effect can be achieved quickly, so that the latter two steps can be initiated simultaneously or subsequently. If the first reaction step is allowed to start first, the amount of the substance of the formula A used can be reduced.
Therefore, in the present invention, the amount of the substance of formula a may vary according to the specific implementation details of the present invention, and usually ranges from 0.001% to 10% to achieve the appropriate interference cancellation or mitigation effect.
In addition, the properties of the compounds of formula A may vary slightly for different specific classes of compounds, and when two or more compounds corresponding to formula A are used in combination, some more pronounced effects can be produced, not only including better interference elimination or mitigation.
In summary, the technical route described in the present invention is summarized as follows:
namely, the present invention provides a detection method or a kit: the method is characterized in that: for detecting the content of 2-imino-1-methylimidazolin-4-one in a sample, comprising the step of contacting a test sample with a compound represented by the above general formula A. In the general formula A, R1, R2, R3 and R4 are groups which can be connected with carbon chains and can be the same or different; r5 is a group that can be attached to a carbon chain.
The invention also provides a kit for the application, wherein the kit components comprise the compound with the general formula A; and further comprising the step of converting the 2-imino-1-methylimidazolin-4-one to hydrogen peroxide; and a step of obtaining the content of 2-imino-1-methylimidazolin-4-one by detecting hydrogen peroxide.
For the structure of formula a, wherein R1, R2, R3, R4 are hydrocarbyl and/or modified hydrocarbyl and/or hydrogen atoms or combinations thereof.
In a preferred embodiment, wherein R1, R2, R3 and R4 are all methyl.
R5 in the compound of the general formula A is one of hydrogen atom, hydroxyl, keto, amino, carboxyl, sulfydryl, alkyl, halogen atom, alkyl, aralkyl, aryl, substituted oxy, substituted amino, substituted alkyl, substituted sulfenyl, cyano and isothiocyanato.
In some preferred examples, R5 is one of hydrogen atom, hydroxyl group, cyano group, carboxyl group, keto group, methoxy group, acetamido group, cyano group, isothiocyanato group, methacryloxy group, and 2-chloroacetamido group.
For the above detection method or kit, better effects can be obtained by using at least 2 compounds of formula A with different structures.
The method for detecting the hydrogen peroxide content in the detection method or the kit is a Trinder's reaction or an electrochemical method.
The detection method or the kit has the application of eliminating or reducing the interference of a compound containing a hydroxyl group connected to an aromatic ring in a sample on a measurement result, in particular eliminating or reducing the interference of 2, 5-dihydroxybenzenesulfonic acid or a salt thereof, or a dopa compound or a salt thereof in the sample on the measurement result.
In addition, the method can be used for eliminating or reducing the interference of the compound containing the hydroxyl group connected to the aromatic heterocyclic ring in the sample on the determination result. And can also be used for eliminating or reducing the interference of hydroxyl groups connected to conjugated double bonds in a sample on the measurement result. And further comprising the use for eliminating or reducing interference of a thiol-containing compound in a sample with the results of the assay; or further comprising the use for eliminating or reducing interference of iron-containing porphyrin protein in a sample with assay results; or further comprises the use for eliminating or reducing interference of ascorbic acid in a sample with the assay results.
Drawings
FIG. 1 is a graph showing the difference between the method of the present invention and the conventional method and the interference effect (relative deviation) for eliminating calcium 2, 5-dihydroxybenzenesulfonate.
Detailed Description
The implementation of the method or kit of the invention will now be described in more detail with reference to some specific examples. It should be noted that the following applications are only specific descriptions for the present invention to help understanding the technology of the present invention, but not to limit the present invention, and according to the known technology, the skilled person in the art should be able to adjust the concentration of each component, substitute the analog, increase or decrease the auxiliary component, adjust the pH of the buffer solution, etc. in the following examples, all fall within the protection scope of the present invention.
Example 1: observing the anti-interference effect of the compound in the general formula A, namely the 4-carboxyl-2, 2, 6, 6-tetramethyl piperidine nitroxide compound.
First, a kit (control group) for detecting 2-imino-1-methylimidazolin-4-one according to the conventional technique was prepared as shown in Table 1A:
note: TOOS is N-ethyl-N- (2-hydroxy-3-sulfopropyl) -3-methylaniline sodium salt.
The 2-imino-1-methylimidazolin-4-one assay kit (panel) according to the technology described in the present invention was prepared as shown in Table 1B,
the detection steps of the kit are as follows: and (3) reacting the sample with a reagent R1 for 3-5 min, adding a reagent R2, measuring the absorbance value after reacting for 5min, and calculating the content of the 2-imino-1-methylimidazoline-4-one in the sample according to the measured absorbance value.
The specific detection parameters and detector are shown in table 1C below:
the test samples were prepared as follows:
the same basic sample is divided into 6 groups, and interferents (2, 5-dihydroxy benzene sulfonic acid calcium) with different concentrations are sequentially added, wherein the specific adding concentrations are shown in table 1D.
The samples were then assayed under the above assay conditions using a control reagent (conventional technique) and a study reagent (technique described in the present invention), and the results of detection of the target analyte (2-imino-1-methylimidazolin-4-one) in each sample and the relative deviation from the assay results of the control base sample containing no interfering substance are shown in Table 1D below.
The test result shows that the calcium 2, 5-dihydroxybenzenesulfonate has very serious interference on the measurement result, but the technical research group described by the invention can greatly eliminate the interference of the interferents on the detection result.
Plotting the relative deviation data in table 1D, fig. 1, allows for a more intuitive comparison of the differences between the control and the study groups. The method has remarkable effect on eliminating the interference of the calcium 2, 5-dihydroxybenzenesulfonate.
Example 2: observing the anti-interference effect of the compound of the general formula A, namely the 4-cyano-2, 2, 6, 6-tetramethyl piperidine nitroxide compound.
The control group reagent of this example is the same as example 1;
the 2-imino-1-methylimidazolin-4-one assay kit (panel) according to the technology described in the present invention was then formulated as shown in Table 2B,
the detection procedure and sample preparation method were the same as in example 1 above;
the results of the measurements are shown in table 2D below:
it can be seen from the table that good anti-interference effect can be obtained when the 4-position of the compound of formula a is other substituent such as cyano using the techniques described in the present invention.
Example 3: the anti-interference effect of the compound of the general formula A, which is a 4-hydroxy-2, 6-diisopropyl piperidine nitroxide compound, is observed.
The control group reagent of this example is the same as example 1;
the 2-imino-1-methylimidazolin-4-one assay kit (panel) according to the technology described in the present invention was then formulated as shown in Table 3B,
the detection procedure and sample preparation method were the same as in example 1 above;
the results of the measurements are shown in table 3D below:
it can be seen from the table that the compounds of formula a, when subjected to the techniques described in the present invention, also have good anti-interference effects when the 2 and 6 positions are other substituents such as isopropyl.
Example 4: the effect of the simultaneous performance of the step of contacting the sample with the substance of formula a and the steps of generating and detecting hydrogen peroxide was observed.
The control group reagent of this example is the same as example 1;
the 2-imino-1-methylimidazolin-4-one assay kit (panel) according to the technology described in the present invention was then prepared as shown in Table 4B,
the sample preparation method is the same as that of the above specific example 1, and the detection conditions of the control group are the same as those of example 1;
the measurement conditions of the investigation group are shown in table 4C below.
The results of the measurements are shown in table 4D below:
as can be seen from the table, when the technique described in the present invention is applied, the three steps of measurement are: under the condition that the step of contacting the compound with the general formula A with the sample, the step of generating hydrogen peroxide and the step of detecting the hydrogen peroxide are carried out simultaneously, good anti-interference effect can still be obtained.
Example 5: the effect of the substance of formula a on eliminating or reducing the interference of methyldopa was observed.
The control group reagent and the study group reagent in this example are the same as those in example 1;
the test procedure and sample formulation method were similar to those described above in example 1, except that the interfering substance was methyldopa and the concentrations of the formulated interferents are shown in Table 5D below.
The results of the measurements are shown in table 5D below:
as can be seen from the table, the effect of the compound of formula a in mitigating methyldopa interference is also very significant when the techniques described in the present invention are used.
Example 6: the effect of the substance of formula A on eliminating or mitigating interference with 1, 3-cyclohexanone was observed.
The control group reagent and the study group reagent in this example are the same as those in example 1;
the test procedure and sample formulation method were similar to those described above for example 1, except that the interfering substance was 1, 3-cyclohexanone and the formulated interfering concentrations are shown in Table 6D below.
The results of the measurements are shown in table 6D below:
as can be seen from the table, the effect of the compounds of formula A in mitigating the above-mentioned interferents is also apparent when the techniques described in the present invention are used.
Example 7: observing the effect of the substance of the general formula A on eliminating or reducing interference of reduced glutathione.
The control group reagent and the study group reagent in this example are the same as those in example 1;
the detection procedure and sample preparation method were similar to those described in example 1 above, except that the interfering substance was reduced glutathione and the prepared interfering concentrations were as shown in Table 7D below.
The results of the measurements are shown in table 7D below:
as can be seen from the table, the interference effect of the compound of formula A in mitigating the above-mentioned interferents is also very significant when the techniques described in the present invention are employed.
Example 8: the effect of the substance of formula a on eliminating or mitigating hemoglobin interference was observed.
The control group reagent and the study group reagent in this example are the same as those in example 1;
the test procedure and sample preparation method were similar to those described above in example 1, except that the interfering substance was hemoglobin and the prepared interfering concentrations were as shown in Table 8D below.
The results of the measurements are shown in Table 8D below:
as can be seen from the table, the effect of the compound of formula A in mitigating the interference of the above interferents is also very significant when the techniques described in the present invention are used.
Example 9: observing the anti-interference effect of the combination of various substances in the general formula A.
The control group reagent of this example is the same as example 1;
the 2-imino-1-methylimidazolin-4-one assay kit of the technology described in the present invention was prepared as shown in Table 9B (investigation group).
The detection procedure and sample preparation method were the same as in example 1 above;
the results of the measurements are shown in table 9D below:
it can be seen from the table that when the technique described in the present invention is used, a better anti-interference effect can be obtained when a combination of two compounds of formula a is used.
Claims (13)
1. A method or kit for detection: the method is characterized in that: for detecting the content of 2-imino-1-methylimidazolin-4-one in a sample, comprising the step of contacting a test sample with a compound represented by the general formula A;
in the general formula A, R1, R2, R3 and R4 are groups which can be connected with carbon chains and can be the same or different; r5 is a group that can be attached to a carbon chain.
2. The detection method or kit according to claim 1, characterized in that: the kit components comprise the compound with the general formula A; and further comprising the step of converting the 2-imino-1-methylimidazolin-4-one to hydrogen peroxide; and a step of obtaining the content of 2-imino-1-methylimidazolin-4-one by detecting hydrogen peroxide.
3. The detection method or kit according to claim 1, characterized in that: wherein R1, R2, R3 and R4 in the formula A are hydrocarbon groups and/or modified hydrocarbon groups and/or hydrogen atoms or a combination thereof.
4. The detection method or kit according to claim 1, characterized in that: wherein R1, R2, R3 and R4 in the formula A are all methyl.
5. The detection method or kit according to claim 1, characterized in that: wherein R5 in the general formula A is one of hydrogen atom, hydroxyl, keto, amino, carboxyl, sulfydryl, alkyl, halogen atom, alkyl, aralkyl, aryl, substituted oxy, substituted amino, substituted alkyl, substituted sulfenyl, cyano and isothiocyanic group.
6. The detection method or kit according to claim 4, characterized in that: wherein R5 in the general formula A is one of hydrogen atom, hydroxyl, cyano, carboxyl, keto, methoxyl, acetamido, cyano, isothiocyanato, methacryloxy and 2-chloroacetamido.
7. The detection method or kit according to claim 1, characterized in that: the kit at least comprises 2 compounds with different structures and the general formula A.
8. The detection method or kit according to claim 1, characterized in that: the method for detecting the content of the hydrogen peroxide is a Trinder's reaction or an electrochemical method.
9. The detection method or kit according to any one of claims 1 to 8, characterized in that: use for eliminating or reducing interference in the assay results by compounds containing hydroxyl groups attached to aromatic rings in a sample.
10. The detection method or kit according to claim 9, characterized in that: use for eliminating or reducing interference of 2, 5-dihydroxybenzenesulfonic acid or a salt thereof, or a dopa-based compound or a salt thereof, in a sample with a measurement result.
11. The detection method or kit according to any one of claims 1 to 8, characterized in that: use for eliminating or reducing interference in a sample from a compound containing a hydroxyl group attached to a heteroaromatic ring with respect to assay results.
12. The detection method or kit according to any one of claims 1 to 8, characterized in that: the method is used for eliminating or reducing the interference of hydroxyl groups connected to conjugated double bonds in a sample on the measurement result.
13. The detection method or kit according to claim 9, characterized in that: also includes at least one of the following applications:
use for eliminating or reducing interference of thiol-containing compounds in a sample with assay results;
or further comprises the use for eliminating or reducing interference of iron-containing porphyrin protein in a sample with assay results.
Or further comprises the use for eliminating or reducing interference of ascorbic acid in a sample with the assay results.
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| CN201828565U (en) * | 2010-08-26 | 2011-05-11 | 上海执诚生物技术有限公司 | Kit for measuring content of blood sugar |
| JP2014155487A (en) * | 2013-01-17 | 2014-08-28 | Toyobo Co Ltd | Biogenic substance measurement method, and composition for measurement |
| JP2016019497A (en) * | 2014-07-15 | 2016-02-04 | 東洋紡株式会社 | Biogenic component measurement method and measurement composition therefor |
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| JP2016019496A (en) * | 2014-07-15 | 2016-02-04 | 東洋紡株式会社 | Biogenic component measurement method and measurement composition therefor |
| CN105572403A (en) * | 2015-12-11 | 2016-05-11 | 济南观韬医疗器械有限公司 | Serum total cholesterol detection kit suitable for full-automatic biochemical analyzer |
| CN109239059A (en) * | 2018-08-30 | 2019-01-18 | 中拓生物有限公司 | A kind of glycated serum protein assay kit and its preparation method and application |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201828565U (en) * | 2010-08-26 | 2011-05-11 | 上海执诚生物技术有限公司 | Kit for measuring content of blood sugar |
| JP2014155487A (en) * | 2013-01-17 | 2014-08-28 | Toyobo Co Ltd | Biogenic substance measurement method, and composition for measurement |
| JP2016019497A (en) * | 2014-07-15 | 2016-02-04 | 東洋紡株式会社 | Biogenic component measurement method and measurement composition therefor |
| JP2016019498A (en) * | 2014-07-15 | 2016-02-04 | 東洋紡株式会社 | Biogenic component measurement method and measurement composition therefor |
| JP2016019496A (en) * | 2014-07-15 | 2016-02-04 | 東洋紡株式会社 | Biogenic component measurement method and measurement composition therefor |
| CN105572403A (en) * | 2015-12-11 | 2016-05-11 | 济南观韬医疗器械有限公司 | Serum total cholesterol detection kit suitable for full-automatic biochemical analyzer |
| CN109239059A (en) * | 2018-08-30 | 2019-01-18 | 中拓生物有限公司 | A kind of glycated serum protein assay kit and its preparation method and application |
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