CN111912843A - Blood coagulation and blood coagulation inhibition experiment result interpretation device - Google Patents
Blood coagulation and blood coagulation inhibition experiment result interpretation device Download PDFInfo
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- CN111912843A CN111912843A CN202010911312.3A CN202010911312A CN111912843A CN 111912843 A CN111912843 A CN 111912843A CN 202010911312 A CN202010911312 A CN 202010911312A CN 111912843 A CN111912843 A CN 111912843A
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- 230000023555 blood coagulation Effects 0.000 title claims abstract description 118
- 230000005764 inhibitory process Effects 0.000 title claims abstract description 21
- 238000002474 experimental method Methods 0.000 title claims description 16
- 230000035931 haemagglutination Effects 0.000 claims abstract description 25
- 238000007781 pre-processing Methods 0.000 claims description 8
- 238000012545 processing Methods 0.000 claims description 7
- 230000011218 segmentation Effects 0.000 claims description 3
- 230000000903 blocking effect Effects 0.000 description 6
- 239000000428 dust Substances 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 3
- 208000007536 Thrombosis Diseases 0.000 description 3
- 229960005486 vaccine Drugs 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 208000002979 Influenza in Birds Diseases 0.000 description 1
- 208000010359 Newcastle Disease Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 206010064097 avian influenza Diseases 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
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- Health & Medical Sciences (AREA)
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- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
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- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The invention discloses a device for interpreting experimental results of hemagglutination and hemagglutination inhibition, which comprises a box body, wherein one end of the box body is provided with a light source; a blood coagulation board inclination component is arranged corresponding to the light source and comprises a blood coagulation board adapter and two support arms; two sides of one end of the blood coagulation board adapter, which is far away from the light source, are respectively hinged with the two support arms; a support arm slide rail fixed on the box body is respectively arranged corresponding to each support arm; the box body also comprises a blood coagulation plate pushing device, and the blood coagulation plate pushing device comprises a screw rod assembly and a motor; the blood coagulation board pushing device can push the blood coagulation board adapter to incline downwards along the adapter slide rail, and the straight light source is arranged in the box body; an image grabbing device is arranged in the box body corresponding to the blood coagulation board adapter. This device can carry out the blood coagulation board slope automatically and snatch the image, avoid with safe risk, labour saving and time saving, reduce the result deviation that subjective factor brought.
Description
Technical Field
The invention belongs to the field of biological detection, and particularly relates to a device for interpreting experimental results of hemagglutination and hemagglutination inhibition.
Background
The hemagglutination and hemagglutination inhibition experiments are mainly used for detecting the antibody level in serum, can be used for judging the immune effect of a human or an animal after vaccine injection, and can also be used for judging the infection state. With the continuous development of the breeding industry, the prevention and control situation of animal epidemic diseases is severe, the common epidemic diseases of poultry animals, such as avian influenza, newcastle disease, and the like, are mainly avoided by injecting vaccines, and the immune effect of the vaccines needs to be determined by a hemagglutination inhibition experiment. At present, almost all the steps are carried out in a manual experiment interpretation mode, and the steps are complicated and tracing cannot be carried out.
In the current implementation scheme, a camera is adopted to photograph at the top of the blood coagulation plate, and the device is integrally inclined, so that the problems of poor photographing effect, poor interpretation accuracy and complex mechanical structure exist; and frequent contact with the reaction plate is required, so that the biological safety risk exists; the original image cannot be reserved and cannot be traced.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides the device for judging the experimental results of hemagglutination and hemagglutination inhibition, which can automatically incline the hemagglutination plate and capture images, further can judge the experimental results after image identification, can avoid pollution and safety risks in real-time manual judgment, is time-saving and labor-saving, and reduces the result deviation caused by subjective factors.
The specific technical scheme is as follows:
a device for judging and reading experimental results of hemagglutination and hemagglutination inhibition comprises a box body, wherein one end of the box body is provided with a light source;
a blood coagulation board inclination component is arranged corresponding to the light source and comprises a blood coagulation board adapter and two support arms; the blood coagulation board adapter comprises a tray which can accommodate the blood coagulation board to be horizontally placed and expose an observation area at the bottom of the blood coagulation board; two sides of one end of the blood coagulation board adapter, which is far away from the light source, are respectively hinged with the two support arms; a support arm slide rail fixed on the box body is respectively arranged corresponding to each support arm; the support arm can slide back and forth along the support arm slide rail corresponding to the light source;
the box body also comprises a blood coagulation board pushing device for driving the blood coagulation board adapter to slide back and forth;
the hemagglutination plate pushing device can push the hemagglutination plate adapter to incline downwards along the adapter slide rail through the pulley by the rotation of the screw rod, and the upper surface of a tray on the hemagglutination plate adapter is provided with a straight surface light source;
the image grabbing device is arranged in the box body corresponding to the blood coagulation board adapter, and when the blood coagulation board adapter inclines downwards along the adapter slide rail, the lens of the image grabbing device can be aligned to the bottom of the blood coagulation board adapter.
The tray preferably has a blood clotting plate securing action to prevent the blood clotting plate from sliding out of the adapter when tilted. The adapter can be provided with a baffle, or a device such as a buckle which can fix the blood coagulation board is arranged.
When the blood coagulation board is inclined and is in a straight surface light source, the blood coagulation board is preferably attached to the light source, interference caused by reflection and refraction of light is reduced, and definition and contrast are ensured.
The image grabbing device is preferably arranged at the other end opposite to the light source and far away from the light source, and the elongated focal length can ensure that one camera grabs the complete image of the whole plate without adopting a plurality of cameras to shoot simultaneously.
When the device is used, the transparent blood coagulation board is fixed on the blood coagulation board adapter, so that the blood coagulation board pushing device pushes the blood coagulation board adapter to slide along the support arm slide rail towards the light source; the camera lens of the image capturing device and the blood coagulation board preferably form an included angle of 90 degrees, a bottom photo of the blood coagulation board is shot, and a flowing state image is captured; the result of blood coagulation or blood coagulation inhibition can be judged from the photographed real-time image.
Further, the blood coagulation board pushing device comprises a lead screw assembly and a motor; the screw rod assembly comprises a screw rod and a screw rod nut connected to the screw rod; the screw nut is fixed at one end of the blood coagulation board adapter, which is far away from the light source; the motor is fixed in the box body and drives the screw rod to rotate.
Still further, the box in still set firmly the mounting panel, the motor be fixed in the mounting panel. The motor and the lead screw are preferably connected through a coupling.
Furthermore, the device also comprises a control circuit board which controls the inclination and the reset of the blood coagulation board adapter, the on and off of the light source and the grabbing of the image.
Still further, the control circuit board also controls the preprocessing of the image. The image preprocessing comprises image single-hole segmentation, binarization processing, tear drop length, wave crest and other parameter calculation.
The software can be designed according to the parameters of image preprocessing, image preprocessing data are received, the final titer is judged according to the judgment rule, and the original image and the interpretation result are stored in the database to generate an experimental report.
Furthermore, the device also comprises a power supply module which provides power supply for all circuit parts.
Further, the image capturing device is a high-definition industrial camera and captures images of the flowing state in real time; the axis of the lens of the high-definition industrial camera is vertical to the plane of the blood coagulation board adapter which is inclined in place.
Furthermore, the adapter slide rails are two and symmetrically arranged on two sides of the blood coagulation board adapter.
Furthermore, the upper end of the box body is provided with a dust blocking door which can horizontally slide back and forth relative to the direction of the light source. When the blood coagulation board needs to be loaded, the dust blocking door is slid to the side far away from the light source, and the blood coagulation board adapter is exposed; when the blood coagulation board inclines, the dust blocking door slides towards the light source to block the bottom of the blood coagulation board, so that the dust is prevented from influencing shooting, and the light blocking board can also play a role in light blocking and is used for condensing light. The dust-proof door can be controlled to open and close by using the control circuit board. Specifically, an initial position, namely an open door position, is determined by a photoelectric sensor; the motor drives the blood coagulation board to slide along a fixed track through the screw rod, and the stop position, namely the door closing position, is determined according to the set inclination angle.
Further, if the dust-proof door is not installed on the device, the upper end of the light source is provided with a preferable light barrier.
The invention has the following beneficial effects:
the invention can realize the automatic inclination of the hemagglutination plate in hemagglutination and hemagglutination inhibition experiments, and carry out clear and accurate image capture, thereby solving the problems that the traditional manual operation needs frequent contact with the reaction plate and brings biological safety risk; and the experimental efficiency and the interpretation accuracy are effectively improved, and the original image can be reserved for tracing. The device can be matched with the image recognition and processing module to judge the experimental result, so that the interpretation precision and efficiency are further improved, and errors caused by subjective factors in manual interpretation are avoided; and can be butted with a laboratory management system, which is beneficial to improving the informatization level of the laboratory.
Drawings
FIG. 1 is a schematic top view of a horizontal blood clot plate of the present invention;
FIG. 2 is a schematic front view of the horizontal state of the internal blood clot plate of the present invention;
FIG. 3 is a schematic front view of the inner blood coagulation plate of the present invention in a state during the inclination thereof;
FIG. 4 is a schematic front view showing a state in which the inner blood coagulation plate of the present invention is completely tilted;
FIG. 5 is a schematic top view of a blood coagulation plate according to the present invention in a state of being tilted;
FIG. 6 shows photographs of the blood coagulation plates obtained by the present invention and the processing procedure thereof.
In the figure: 1. a light source; 2. a blood clot plate adapter; 3. a support arm; 4. a support arm slide rail; 4.1, a sliding block; 5. a motor; 6. a lead screw; 7. an adapter slide rail; 8. a dust-blocking door; 9. a high definition industrial camera; 10. mounting a plate; 11. a coupling; 12. a blood coagulation plate.
Detailed Description
The principles and features of this invention are described below in conjunction with examples, which are set forth to illustrate, but are not to be construed to limit the scope of the invention.
A device for interpreting hemagglutination and hemagglutination inhibition experimental results, as shown in figures 1-5, comprises a box body with an opening at the upper part, wherein one end of the box body is provided with a light source 1;
a blood coagulation board tilting assembly is arranged corresponding to the light source 1, and comprises a blood coagulation board adapter 2 and a support arm 3; the blood coagulation board adapter 2 comprises a tray which can accommodate the blood coagulation board 12 to be horizontally and fixedly placed and expose an observation area at the bottom of the blood coagulation board 12; two sides of one end of the blood coagulation board adapter 2, which is far away from the light source 1, are respectively hinged with the two support arms 3; a support arm slide rail 4 fixed on the box body is respectively arranged corresponding to each support arm 3; the support arm 3 can slide back and forth along the support arm slide rail 4 corresponding to the light source 1 through a slide block 4.1;
the box body also comprises a blood coagulation plate pushing device for driving the blood coagulation plate adapter to slide back and forth, and the blood coagulation plate pushing device comprises a lead screw assembly and a motor 5; the lead screw assembly comprises a lead screw 6 and a lead screw nut connected to the lead screw 6; the screw nut is fixed at one end of the blood coagulation board adapter 2 far away from the light source 1; the box body is internally and fixedly provided with an installation plate 10, and the motor 5 is fixed on the installation plate 10; the motor 5 is connected with the lead screw 6 through the coupler 11 and drives the lead screw 6 to rotate;
an adapter slide rail 7 is fixedly arranged in the box body, a pulley corresponding to the adapter slide rail 7 is arranged on the side edge of one end, close to the light source 1, of the blood coagulation board adapter 2, and the blood coagulation board pushing device can push the blood coagulation board adapter 2 to incline downwards along the adapter slide rail 7 and enable the upper surface of a tray on the blood coagulation board adapter 2 to be straight and attached to the light source 1;
the upper end of the box body is provided with a dust blocking door 8 which can horizontally slide back and forth relative to the direction of the light source 1;
the high-definition industrial camera 9 is arranged in the box body corresponding to the blood coagulation board adapter 2, when the blood coagulation board adapter 2 inclines downwards along the adapter slide rail 7, the lens of the high-definition industrial camera 9 can be aligned to the position of the bottom tray of the blood coagulation board adapter 2, and the axis of the high-definition industrial camera 9 is vertical to the plane of the blood coagulation board adapter 2 after the lens inclines to the right position;
the device also comprises a control circuit board, wherein the control circuit board controls the inclination and the reset of the blood coagulation board adapter 2, the opening and the closing of the light source 1, the image capture and the image preprocessing; the image preprocessing comprises image single-hole segmentation, binarization processing, tear drop length and wave crest parameter calculation; the control circuit can also control the opening and closing of the dust-proof door 8 through a photoelectric sensor;
the device also comprises a power supply module which supplies power for all the circuits.
When the device is used, the dust-proof door 8 is opened, the transparent blood coagulation board 12 is fixed on the blood coagulation board adapter 2, the motor 5 is started, the screw rod component pushes the blood coagulation board adapter 2 to slide towards the light source 1 along the support arm slide rail 4, when the blood coagulation board adapter 2 reaches the adapter slide rail 7, the pulleys on the side edge slide along the adapter slide rail 7, so that the blood coagulation board adapter is attached to the straight light source 1, the light source 1 is in an open state, uniform background light is provided, and the light is directly irradiated to the blood coagulation board; the camera lens of the high-definition industrial camera 9 and the blood coagulation board preferably form an included angle of 90 degrees, and a picture of the bottom of the blood coagulation board 12 is shot to capture a flowing state image; the high-definition industrial camera 9 is connected with a computer, and images are preprocessed and stored in the computer for manual interpretation; or the design result analysis software judges the final titer according to the judgment rule, and the original image and the interpretation result are stored in the database to generate an experiment report.
As shown in FIG. 6, the photographs of the blood coagulation plates and the processing flow thereof obtained by the present invention can clearly grasp the flow state of the sample when the blood coagulation plates are tilted in place, and further data processing can be performed.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (10)
1. The device for judging and reading the experimental results of hemagglutination and hemagglutination inhibition is characterized by comprising a box body, wherein one end of the box body is provided with a light source (1);
arranging a blood coagulation board tilting assembly corresponding to the light source (1), wherein the blood coagulation board tilting assembly comprises a blood coagulation board adapter (2) and a support arm (3); the blood coagulation board adapter (2) comprises a tray which can accommodate the blood coagulation board (12) to be horizontally placed and expose an observation area at the bottom of the blood coagulation board (12); two sides of one end of the blood coagulation board adapter (2) far away from the light source (1) are respectively hinged with the two support arms (3); a support arm slide rail (4) fixed on the box body is respectively arranged corresponding to each support arm (3); the support arm (3) can slide back and forth along the support arm slide rail (4);
the box body also comprises a blood coagulation board pushing device for driving the blood coagulation board adapter (2) to slide back and forth;
an adapter slide rail (7) is fixedly arranged in the box body, a pulley corresponding to the adapter slide rail (7) is arranged on the side edge of one end, close to the light source (1), of the blood coagulation board adapter (2), and the blood coagulation board pushing device can push the blood coagulation board adapter (2) to incline downwards along the adapter slide rail (7) and enable a surface light source (1) on the upper surface of a tray on the blood coagulation board adapter (2);
the image grabbing device is arranged in the box body corresponding to the blood coagulation board adapter (2), and when the blood coagulation board adapter (2) inclines downwards along the adapter slide rail (7), the lens of the image grabbing device can be aligned to the bottom of the blood coagulation board adapter (2).
2. The device for interpreting results of hemagglutination and hemagglutination inhibition experiments according to claim 1, wherein the hemagglutination plate pushing device comprises a screw assembly and a motor (5); the lead screw assembly comprises a lead screw (6) and a lead screw nut connected to the lead screw (6); the screw nut is fixed at one end of the blood coagulation board adapter (2) far away from the light source (1); the motor (5) is fixed in the box body and drives the screw rod (6) to rotate.
3. The apparatus for interpreting results of blood coagulation and blood coagulation inhibition experiments according to claim 2, wherein a mounting plate (10) is further fixedly arranged in the case, and the motor (5) is fixed on the mounting plate (10).
4. The apparatus for interpreting results of blood coagulation and blood coagulation inhibition experiments according to any one of claims 1 to 3, further comprising a control circuit board which controls the tilting and resetting of the blood coagulation plate adapter (2), the turning on and off of the light source (1) and the capturing of images.
5. The device for interpreting results of blood coagulation and blood coagulation inhibition experiments according to claim 4, wherein said control circuit board also controls the preprocessing of images.
6. The apparatus for interpreting results of blood coagulation and blood coagulation inhibition experiments according to claim 5, wherein the preprocessing of the image comprises image single-hole segmentation, binarization processing, tear drop length and peak parameter calculation.
7. The device for interpreting results of blood coagulation and blood coagulation inhibition experiments according to any one of claims 1-3, further comprising a power supply module for supplying power to all circuits.
8. The apparatus for interpreting results of blood coagulation and blood coagulation inhibition experiments according to any one of claims 1 to 3, characterized in that the image-capturing device is a high definition industrial camera (9), and the lens axis of the high definition industrial camera (9) is perpendicular to the plane of the blood coagulation plate adaptor (2) after it is tilted into place.
9. The device for interpreting results of blood coagulation and blood coagulation inhibition experiments according to any one of claims 1 to 3, wherein the two adapter slide rails (7) are symmetrically arranged on both sides of the blood coagulation plate adapter (2).
10. The apparatus for interpreting results of blood coagulation and blood coagulation inhibition experiments according to claim 1 or 2, wherein the upper end of the case is provided with a dust-proof door (8) which can horizontally slide back and forth with respect to the direction of the light source (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010911312.3A CN111912843A (en) | 2020-09-02 | 2020-09-02 | Blood coagulation and blood coagulation inhibition experiment result interpretation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010911312.3A CN111912843A (en) | 2020-09-02 | 2020-09-02 | Blood coagulation and blood coagulation inhibition experiment result interpretation device |
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| CN111912843A true CN111912843A (en) | 2020-11-10 |
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| CN202010911312.3A Pending CN111912843A (en) | 2020-09-02 | 2020-09-02 | Blood coagulation and blood coagulation inhibition experiment result interpretation device |
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4290997A (en) * | 1978-02-28 | 1981-09-22 | Kommandiittiyhtio Finnpipette Osmo A. Suovaniemi | Apparatus for automatic measurement of the results of agglutination tests |
| CN201382945Y (en) * | 2009-03-16 | 2010-01-13 | 杨傲冰 | Epidemic disease hemagglutination detecting system |
| CN105974145A (en) * | 2016-05-17 | 2016-09-28 | 山东栋梁科技设备有限公司 | Full-automatic Newcastle disease detector |
| CN205655823U (en) * | 2016-04-26 | 2016-10-19 | 科络普线束技术（绵阳）有限公司 | Novel optical detection equipment |
| CN106661764A (en) * | 2014-07-28 | 2017-05-10 | 赛诺菲巴斯德维思设计公司 | Automated imaging and analysis of the hemagglutination inhibition assay (HAI) |
| CN107024061A (en) * | 2017-04-28 | 2017-08-08 | 南京创维家用电器有限公司 | A kind of thawing apparatus, refrigerator and defreezing method |
| CN209327219U (en) * | 2018-08-30 | 2019-08-30 | 济南继德生物科技有限公司 | A kind of continuous interpretation device of test paper |
| CN212228758U (en) * | 2020-09-02 | 2020-12-25 | 北京哈美顿生物科技有限公司 | Blood coagulation and blood coagulation inhibition experiment result interpretation device |
-
2020
- 2020-09-02 CN CN202010911312.3A patent/CN111912843A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4290997A (en) * | 1978-02-28 | 1981-09-22 | Kommandiittiyhtio Finnpipette Osmo A. Suovaniemi | Apparatus for automatic measurement of the results of agglutination tests |
| CN201382945Y (en) * | 2009-03-16 | 2010-01-13 | 杨傲冰 | Epidemic disease hemagglutination detecting system |
| CN106661764A (en) * | 2014-07-28 | 2017-05-10 | 赛诺菲巴斯德维思设计公司 | Automated imaging and analysis of the hemagglutination inhibition assay (HAI) |
| CN205655823U (en) * | 2016-04-26 | 2016-10-19 | 科络普线束技术（绵阳）有限公司 | Novel optical detection equipment |
| CN105974145A (en) * | 2016-05-17 | 2016-09-28 | 山东栋梁科技设备有限公司 | Full-automatic Newcastle disease detector |
| CN107024061A (en) * | 2017-04-28 | 2017-08-08 | 南京创维家用电器有限公司 | A kind of thawing apparatus, refrigerator and defreezing method |
| CN209327219U (en) * | 2018-08-30 | 2019-08-30 | 济南继德生物科技有限公司 | A kind of continuous interpretation device of test paper |
| CN212228758U (en) * | 2020-09-02 | 2020-12-25 | 北京哈美顿生物科技有限公司 | Blood coagulation and blood coagulation inhibition experiment result interpretation device |
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