CN111919709A - Cow dung basic carrier culture medium containing plant growth-promoting bacteria and preparation method thereof - Google Patents
Cow dung basic carrier culture medium containing plant growth-promoting bacteria and preparation method thereof Download PDFInfo
- Publication number
- CN111919709A CN111919709A CN202010916625.8A CN202010916625A CN111919709A CN 111919709 A CN111919709 A CN 111919709A CN 202010916625 A CN202010916625 A CN 202010916625A CN 111919709 A CN111919709 A CN 111919709A
- Authority
- CN
- China
- Prior art keywords
- cow dung
- growth
- promoting bacteria
- basic carrier
- promoting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000003608 fece Anatomy 0.000 title claims abstract description 67
- 241000894006 Bacteria Species 0.000 title claims abstract description 35
- 239000001963 growth medium Substances 0.000 title claims abstract description 25
- 238000002360 preparation method Methods 0.000 title claims abstract description 10
- 241000196324 Embryophyta Species 0.000 claims abstract description 41
- 240000008042 Zea mays Species 0.000 claims abstract description 23
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 23
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 23
- 235000005822 corn Nutrition 0.000 claims abstract description 23
- 239000010902 straw Substances 0.000 claims abstract description 23
- 238000000855 fermentation Methods 0.000 claims abstract description 20
- 230000004151 fermentation Effects 0.000 claims abstract description 20
- 239000002361 compost Substances 0.000 claims abstract description 15
- 235000015097 nutrients Nutrition 0.000 claims abstract description 15
- 239000000758 substrate Substances 0.000 claims abstract description 15
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000004021 humic acid Substances 0.000 claims abstract description 13
- 241001453380 Burkholderia Species 0.000 claims abstract description 12
- 230000001737 promoting effect Effects 0.000 claims abstract description 12
- 241000589151 Azotobacter Species 0.000 claims abstract description 11
- 239000002131 composite material Substances 0.000 claims abstract description 10
- 244000005700 microbiome Species 0.000 claims abstract description 8
- 235000019362 perlite Nutrition 0.000 claims abstract description 8
- 239000010451 perlite Substances 0.000 claims abstract description 8
- 235000019354 vermiculite Nutrition 0.000 claims abstract description 8
- 239000010455 vermiculite Substances 0.000 claims abstract description 8
- 229910052902 vermiculite Inorganic materials 0.000 claims abstract description 8
- 239000000203 mixture Substances 0.000 claims abstract description 6
- 230000012010 growth Effects 0.000 claims abstract description 5
- 108090000790 Enzymes Proteins 0.000 claims abstract description 4
- 102000004190 Enzymes Human genes 0.000 claims abstract description 4
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 4
- 239000002994 raw material Substances 0.000 claims abstract description 4
- 239000002609 medium Substances 0.000 claims abstract 4
- 241000283690 Bos taurus Species 0.000 claims description 63
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 239000000463 material Substances 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 11
- 239000003337 fertilizer Substances 0.000 claims description 7
- 238000002156 mixing Methods 0.000 claims description 7
- 230000008635 plant growth Effects 0.000 claims description 6
- 239000010871 livestock manure Substances 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 239000000654 additive Substances 0.000 claims description 4
- 230000000996 additive effect Effects 0.000 claims description 4
- 230000000813 microbial effect Effects 0.000 claims description 4
- 238000009264 composting Methods 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 230000003203 everyday effect Effects 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 3
- 238000005273 aeration Methods 0.000 claims description 2
- 241000607479 Yersinia pestis Species 0.000 abstract description 7
- 238000004519 manufacturing process Methods 0.000 abstract description 7
- 201000010099 disease Diseases 0.000 abstract description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 4
- 229930192334 Auxin Natural products 0.000 abstract description 2
- 208000035240 Disease Resistance Diseases 0.000 abstract description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 abstract description 2
- 239000002363 auxin Substances 0.000 abstract description 2
- 230000002708 enhancing effect Effects 0.000 abstract description 2
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 abstract description 2
- 238000011177 media preparation Methods 0.000 abstract description 2
- 229910052698 phosphorus Inorganic materials 0.000 abstract description 2
- 239000011574 phosphorus Substances 0.000 abstract description 2
- 230000003248 secreting effect Effects 0.000 abstract description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 239000002689 soil Substances 0.000 description 5
- 239000007788 liquid Substances 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 235000013311 vegetables Nutrition 0.000 description 4
- 239000001888 Peptone Substances 0.000 description 3
- 108010080698 Peptones Proteins 0.000 description 3
- 235000015278 beef Nutrition 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 235000019319 peptone Nutrition 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- 241000244206 Nematoda Species 0.000 description 2
- 238000012271 agricultural production Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000009335 monocropping Methods 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 241000086906 Cupriavidus sp. Species 0.000 description 1
- 241000589774 Pseudomonas sp. Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 238000010413 gardening Methods 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 235000013379 molasses Nutrition 0.000 description 1
- 239000010908 plant waste Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Soil Sciences (AREA)
- Fertilizers (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention relates to the technical field of culture medium preparation, and discloses a cow dung basic carrier culture medium containing plant growth-promoting bacteria and a preparation method thereof, wherein a basic carrier of the medium comprises the following raw materials in percentage by mass: 60-65% of cow dung, 10-15% of corn straw, 5-10% of vermiculite and 5-10% of perlite; wherein, the cow dung and the corn straws are subjected to compost fermentation, and the fermented and decomposed basic carrier is compounded with growth-promoting bacteria, plant nutrient elements and humic acid; the growth-promoting bacteria in the substrate are a composite microorganism growth-promoting microbial inoculum formed by combining dozens of microorganisms and biological enzymes, and the composite microorganism growth-promoting microbial inoculum is prepared from azotobacter: burkholderia ﹦ 1: 1, the effective viable count is more than 80 hundred million cuf/g, and the composition has the functions of secreting auxin, dissolving phosphorus, enhancing disease resistance of plants, resisting stress, and the like and promoting the growth of the plants. The invention can solve the pollution problem of cow dung and corn stalk, and has low production cost, less pest and disease damage, high seedling quality, less damage to high-quality farmland and wide application range.
Description
Technical Field
The invention relates to the technical field of culture medium preparation, in particular to a cow dung basic carrier culture medium containing plant growth-promoting bacteria and a preparation method thereof.
Background
With the rapid development of facility gardening, the soilless culture technology and the plug seedling technology are popularized and applied in a large area and a large range, which greatly promotes the research, development and application of the solid culture medium. In soilless culture, compared with water culture, the solid matrix culture facility is simple, the investment cost is low, and the use is very common.
The substrate cultivation is a cultivation technique for cultivating crops by using a solid substrate and a nutrient solution without using soil, thereby achieving high yield. The substrate cultivation and facility agriculture are combined, the agriculture is not restricted by the geographical environment, the substrate cultivation is used as an advanced agricultural technology, the pollution-free green vegetable reliable production method is realized at present, the plant is not polluted by plant diseases and insect pests such as soil pests, fungi and nematodes and weeds, and the method has the advantages of high controllability, high fertilizer utilization rate, capability of avoiding soil-borne plant diseases and insect pests, continuous cropping obstacles, secondary salinization of soil and the like.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides the cow dung basic carrier culture medium containing the plant growth-promoting bacteria and the preparation method thereof, which are used for plant culture or seedling culture medium and have the advantages that the plant is not polluted by pests and diseases such as soil pests, fungi, nematodes and the like and weeds, the controllability is high, the utilization rate of the fertilizer is high, the soil-borne pests and continuous cropping obstacles are avoided, and the secondary salinization of the soil is avoided.
(II) technical scheme
In order to achieve the purpose, the invention provides the following technical scheme:
a cow dung basic carrier cultivation substrate containing plant growth-promoting bacteria comprises the following raw materials in percentage by mass: 60-65% of cow dung, 10-15% of corn straw, 5-10% of vermiculite and 5-10% of perlite;
wherein, the cow dung and the corn straws are subjected to compost fermentation, and the fermented and decomposed basic carrier is compounded with growth-promoting bacteria, plant nutrient elements and humic acid.
Preferably, redundant moisture is removed by the cow dung, the moisture content of the cow dung is 60%, and the particle size of the dried corn straw is 16-25 mm.
Preferably, the growth-promoting bacteria are a composite microbial growth-promoting agent formed by combining tens of microorganisms and biological enzymes, and are prepared from azotobacter: burkholderia ﹦ 1: 1, the effective viable count is more than 80 hundred million cuf/g, and the addition amount is 0.3 percent of the mass of the material.
Preferably, the plant nutrient element (N: P: K) ratio is 3:3: 9; the humic acid is a conventional additive in the fertilizer industry.
Preferably, the addition amount of the growth-promoting bacteria is 0.3% of the cow dung substrate carrier.
A preparation method of a cow dung basic carrier culture medium containing plant growth-promoting bacteria comprises the following steps:
the method comprises the following steps: naturally airing or drying the cow dung to remove redundant water, and crushing the cow dung to obtain cow dung powder when the water content of the cow dung is 60%;
step two: mixing the cattle manure residues and the corn straws according to the weight ratio of (80-85) to (15-20);
step three: the cow dung and the corn straws are subjected to compost fermentation, and the fermented and decomposed materials are composed of growth-promoting bacteria, plant nutrient elements and humic acid;
step four: and adding 5-10% of vermiculite and 5-10% of perlite into the obtained fermentation mixture, and uniformly stirring.
Preferably, the time for fermenting the cow dung and the corn straw compost is 10-15 days, and the temperature of the compost is 40-70 ℃.
Preferably, in the composting treatment process of the cow dung and the corn straws, turning the compost on the 4 th day and the 12 th day, and automatically aerating every day.
(III) advantageous technical effects
Compared with the prior art, the invention has the following beneficial technical effects:
in the prior art, the fermentation material obtained by mixing the fresh cow dung and the plant waste chips can be independently used for planting plants, and most plants grow well;
according to the invention, the dried vegetable scrap corn straw and the fresh cow dung are mixed to reduce the water content of the cow dung, and then fermentation is carried out, so that the production procedures can be reduced, the production period can be shortened, the yield can be improved, the utilization of waste resources can be promoted, and the production cost can be obviously reduced;
meanwhile, loose vermiculite which does not contain organic colloid substances, has low nutrient and salt content, strong water absorption, water retention and water permeability and relatively low price and perlite which can improve the air permeability of the substrate are selected to be compounded with the fermentation material of the cow dung, so that the fermentation material of the pure cow dung and other substrates can make up for the deficiencies of each other, and the composite substrate which has excellent property and is suitable for the cultivation and seedling raising of various plants is produced;
the culture medium is suitable for garden, vegetable production and agricultural production, is also suitable for cultivation in courtyards, balconies and roofs of urban families, is low in price and cost-saving, and plays a role in promoting urban greening.
Detailed Description
The strains involved in the invention are all derived from China center for the culture Collection of Industrial microorganisms (GSICC);
the nitrogen-fixing bacteria (Pseudomonas sp.) are given the following commercial numbers: GSICC 30114;
the commercial number of Burkholderia (Cupriavidus sp.) is: GSICC 31606;
the invention is further illustrated by the following examples;
the first embodiment is as follows:
a cow dung basic carrier cultivation substrate containing plant growth-promoting bacteria comprises the following raw materials in percentage by mass: 60-65% of cow dung, 10-15% of corn straw, 5-10% of vermiculite and 5-10% of perlite;
wherein, the cow dung and the corn straws are subjected to compost fermentation, and the fermented and decomposed materials are compounded with growth-promoting bacteria, plant nutrient elements and humic acid to form the fertilizer;
the mass percentage of the materials of the growth-promoting bacteria is 0.3 percent;
the proportion of plant nutrient elements (N: P: K) is 3:3:9, and the mass percentage of the plant nutrient elements is 12.5%;
the material mass percentage of the humic acid is 10.5 percent, and the humic acid is a conventional additive in the fertilizer industry;
preferably, redundant moisture in the cow dung is removed, the moisture content of the cow dung is 60%, and the particle size of the dried corn straw is 16-25 mm;
wherein, the growth-promoting bacterium is developed by biology of scientific academy of Gansu province, and the composite microorganism growth-promoting bacterium agent formed by combining dozens of microorganisms and biological enzymes is prepared from azotobacter: burkholderia ﹦ 1: 1, the effective viable count is more than 80 hundred million cuf/g, and the addition amount of the viable count is 0.3 percent of the mass of the material;
the growth-promoting bacterium has the effects of secreting auxin, dissolving phosphorus, enhancing disease resistance of plants, resisting stress, and promoting plant growth in various aspects;
the culture medium used in the preparation of the growth-promoting bacteria azotobacter and burkholderia comprises the following components in proportion: 10g of beef extract, 10g of peptone, 5g of NaCl and 1000mL of water, wherein the pH value is 7.2-7.4;
a preparation method of a cow dung basic carrier culture medium containing plant growth-promoting bacteria comprises the following steps:
the method comprises the following steps: naturally airing or drying the cow dung to remove redundant water, and crushing the cow dung to obtain cow dung powder when the water content of the cow dung is 60%;
step two: mixing the cattle manure residues and the corn straws according to the weight ratio of (80-85) to (15-20); wherein the cattle manure residues are solid cattle manure residues obtained by carrying out solid-liquid separation on flushing sewage of a large-scale cattle farm;
step three: fully mixing straw powder and cow dung powder, and adding a composite microbial growth-promoting microbial inoculum produced by biological research institute of science institute of Gansu province for fermentation treatment;
the composite microbial growth promoting agent is prepared by the following steps:
preparing seed liquid of azotobacter and Burkholderia, wherein the culture medium used by the seed liquid comprises the following components in proportion: 10g of beef extract, 10g of peptone, 5g of NaCl, 15g of agar and 1000mL of tap water, wherein the pH value is 7.2-7.4;
respectively inoculating azotobacter and Burkholderia bacillus into a slant culture medium for activation, culturing for 24 hours at 30 ℃, taking out the activated azotobacter and Burkholderia bacillus from a culture medium plate by using an inoculating needle, streaking and separating, culturing for 24 hours at 30 ℃, selecting a single colony with good growth vigor, inoculating the single colony into a liquid culture medium by using the inoculating needle, and performing shake culture for 24 hours at 30 ℃ in a triangular flask;
③ the enlarged culture of azotobacter and Burkholderia: 40L of culture medium (the components of the culture medium are: 10g of beef extract, 10g of peptone, 5g of NaCl and 1000mL of water) and 5Kg of molasses are added into a 50L fermentation tank, the pH value is adjusted to 7.2, the mixture is sterilized at 100 ℃ for 2 hours, after cooling, 500mL of azotobacter and Burkholderia seed liquid are respectively inoculated into the mixture, and the culture conditions are as follows: continuously stirring at the temperature of 30 ℃ and the stirring speed of 150rpm for 24 hours;
fourthly, the fermentation liquor of the azotobacter and the burkholderia which are subjected to expanded culture is mixed according to the weight ratio of 1: 1, mixing to ensure that the effective viable count is more than 80 hundred million cuf/g;
taking the cow dung straw compost fermentation product as a substrate, and adding the composite growth-promoting fermentation liquor, plant nutrient elements and humic acid to obtain the cow dung basic carrier culture substrate containing the plant growth-promoting bacteria;
wherein the mass of the added material of the composite growth promoting fermentation broth is 0.3%;
the proportion of the added plant nutrient elements (N: P: K) is 3:3:9, and the mass of the added plant nutrient elements is 12.5%;
the mass of the added humic acid is 10.5 percent, and the added humic acid is a conventional additive in the fertilizer industry;
the time of fermenting cow dung and corn straw compost is 10-15 days, the temperature of the compost is 40-70 ℃, the compost is turned over on the 4 th day and the 12 th day in the composting process, and automatic aeration is performed every day;
step four: adding 5-10% of vermiculite and 5-10% of perlite into the obtained fermentation mixture, and uniformly stirring;
the using method comprises the following steps: directly mixing the prepared cow dung basic carrier culture medium containing the plant growth-promoting bacteria with plant seeds, or dipping roots of the plants and irrigating the roots;
through experiments, the culture medium prepared in the first embodiment is suitable for being used in garden and vegetable production and agricultural production, is also suitable for cultivation in courtyards, balconies and roofs of urban families, is low in price, saves cost, and plays a role in promoting urban greening.
Claims (8)
1. A cow dung basic carrier cultivation substrate containing plant growth-promoting bacteria is characterized in that a basic carrier of the substrate comprises the following raw materials in percentage by mass: 60-65% of cow dung, 10-15% of corn straw, 5-10% of vermiculite and 5-10% of perlite;
wherein, the cow dung and the corn straws are subjected to compost fermentation, and the fermented and decomposed basic carrier is compounded with growth-promoting bacteria, plant nutrient elements and humic acid.
2. The plant growth promoting bacteria-containing cow dung basic carrier culture medium as claimed in claim 1, wherein the cow dung is free of excess water, the moisture content of the cow dung is 60%, and the particle size of the dried corn stalks is 16-25 mm.
3. The cow dung-based carrier cultivation medium containing plant growth-promoting bacteria as claimed in claim 2, wherein the growth-promoting bacteria are a composite microbial growth-promoting microbial inoculum consisting of tens of microorganisms and biological enzymes, and are selected from the group consisting of azotobacter: burkholderia ﹦ 1: 1, the effective viable count is more than 80 hundred million cuf/g, and the addition amount is 0.3 percent of the mass of the material.
4. The plant growth promoting bacteria-containing cow dung-based carrier cultivation medium as claimed in claim 3, wherein the plant nutrient elements (N: P: K) ratio is 3:3: 9; the humic acid is a conventional additive in the fertilizer industry.
5. The cow dung basic carrier cultivation medium containing the plant growth promoting bacteria, according to the claim 4, is characterized in that the addition amount of the growth promoting bacteria is 0.3% of the cow dung basic carrier.
6. A preparation method of a cow dung basic carrier culture medium containing plant growth-promoting bacteria is characterized by comprising the following steps:
the method comprises the following steps: naturally airing or drying the cow dung to remove redundant water, and crushing the cow dung to obtain cow dung powder when the water content of the cow dung is 60%;
step two: mixing the cattle manure residues and the corn straws according to the weight ratio of (80-85) to (15-20);
step three: the cow dung and the corn straws are subjected to compost fermentation, and the fermented and decomposed materials are composed of growth-promoting bacteria, plant nutrient elements and humic acid;
step four: and adding 5-10% of vermiculite and 5-10% of perlite into the obtained fermentation mixture, and uniformly stirring.
7. The preparation method of the cow dung basic carrier culture medium containing the plant growth promoting bacteria as claimed in claim 6, wherein the fermentation treatment time of the cow dung and the corn straw compost is 10-15 days, and the temperature of the compost is 40-70 ℃.
8. The method for preparing the cow dung basic carrier culture medium containing the plant growth promoting bacteria as claimed in claim 7, wherein the cow dung and the corn straw are turned over on the 4 th day and the 12 th day in the composting process, and automatic aeration is performed every day.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010916625.8A CN111919709A (en) | 2020-09-03 | 2020-09-03 | Cow dung basic carrier culture medium containing plant growth-promoting bacteria and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010916625.8A CN111919709A (en) | 2020-09-03 | 2020-09-03 | Cow dung basic carrier culture medium containing plant growth-promoting bacteria and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111919709A true CN111919709A (en) | 2020-11-13 |
Family
ID=73309144
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010916625.8A Pending CN111919709A (en) | 2020-09-03 | 2020-09-03 | Cow dung basic carrier culture medium containing plant growth-promoting bacteria and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111919709A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113122483A (en) * | 2021-05-31 | 2021-07-16 | 哈尔滨工业大学 | Rapid heat-producing microbial inoculum for aerobic composting of corn straws and application of rapid heat-producing microbial inoculum |
| CN113508739A (en) * | 2021-07-13 | 2021-10-19 | 湖州壤之沃生物技术有限公司 | Organic nutritional type culture medium and preparation method thereof |
| CN114916400A (en) * | 2022-04-19 | 2022-08-19 | 淮阴工学院 | Crassulaceae succulent plant cultivation medium prepared from steam-exploded camphorwood chips |
| CN114982589A (en) * | 2022-04-21 | 2022-09-02 | 甘肃省科学院生物研究所 | Corn seeding time probiotic composite matrix and preparation method, matrix cup and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR19990080372A (en) * | 1998-04-16 | 1999-11-05 | 이영하 | Microbial preparation for manufacturing compost and its manufacturing method |
| CN103396265A (en) * | 2013-08-03 | 2013-11-20 | 许云刚 | Preparation method of trace element-rich bio-fertilizer for chrysanthemum cultivation |
| CN105794530A (en) * | 2014-12-29 | 2016-07-27 | 杭州满庭芳生物科技有限公司 | Method for producing plant cultivating and seedling raising matrix by using cattle manure and use method for matrix |
| CN106631551A (en) * | 2016-09-29 | 2017-05-10 | 山西宁邦生物肥业有限公司 | Plant-growth-promoting bio-organic fertilizer and preparation method |
| CN108823134A (en) * | 2018-07-25 | 2018-11-16 | 江苏天象生物科技有限公司 | A kind of corn stover decomposing agent and preparation method thereof |
-
2020
- 2020-09-03 CN CN202010916625.8A patent/CN111919709A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR19990080372A (en) * | 1998-04-16 | 1999-11-05 | 이영하 | Microbial preparation for manufacturing compost and its manufacturing method |
| CN103396265A (en) * | 2013-08-03 | 2013-11-20 | 许云刚 | Preparation method of trace element-rich bio-fertilizer for chrysanthemum cultivation |
| CN105794530A (en) * | 2014-12-29 | 2016-07-27 | 杭州满庭芳生物科技有限公司 | Method for producing plant cultivating and seedling raising matrix by using cattle manure and use method for matrix |
| CN106631551A (en) * | 2016-09-29 | 2017-05-10 | 山西宁邦生物肥业有限公司 | Plant-growth-promoting bio-organic fertilizer and preparation method |
| CN108823134A (en) * | 2018-07-25 | 2018-11-16 | 江苏天象生物科技有限公司 | A kind of corn stover decomposing agent and preparation method thereof |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113122483A (en) * | 2021-05-31 | 2021-07-16 | 哈尔滨工业大学 | Rapid heat-producing microbial inoculum for aerobic composting of corn straws and application of rapid heat-producing microbial inoculum |
| CN113508739A (en) * | 2021-07-13 | 2021-10-19 | 湖州壤之沃生物技术有限公司 | Organic nutritional type culture medium and preparation method thereof |
| CN114916400A (en) * | 2022-04-19 | 2022-08-19 | 淮阴工学院 | Crassulaceae succulent plant cultivation medium prepared from steam-exploded camphorwood chips |
| CN114982589A (en) * | 2022-04-21 | 2022-09-02 | 甘肃省科学院生物研究所 | Corn seeding time probiotic composite matrix and preparation method, matrix cup and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102432393B (en) | Composite organic soil for raising rice seedlings | |
| CN111919709A (en) | Cow dung basic carrier culture medium containing plant growth-promoting bacteria and preparation method thereof | |
| CN102503652B (en) | Preparation method of organic compound soil for rice seedling raising | |
| CN1648107A (en) | Biological organic fertilizer and its preparing method | |
| CN104774072A (en) | Rice seedling raising organic matrix | |
| CN110106126A (en) | One plant of colloid bacillus cereus and its preparing the application in saline-alkali soil conditioner | |
| CN102503654A (en) | Seedling raising substrate for mechanically-transplanting rice | |
| CN102503653B (en) | Organic compound soil for rice seedling raising | |
| CN105503333A (en) | Seedling raising medium special for organic rice | |
| CN104744182A (en) | Tobacco water retention biological organic fertilizer and preparation method thereof | |
| CN112501072A (en) | Crop rhizosphere soil conditioner and preparation method and application thereof | |
| CN112457125A (en) | Banana wilt prevention bio-organic fertilizer and preparation method and application thereof | |
| CN112094149A (en) | Preparation method of composite microbial organic fertilizer based on cow dung and wheat straw | |
| CN111393206A (en) | Organic-inorganic full-element biological organic fertilizer containing composite bacteria and application thereof | |
| CN103173387A (en) | Growth-promoting bacteria for facilitating growth of rape and microbial organic fertilizer | |
| CN111226742B (en) | Rice seedling raising substrate and preparation method thereof | |
| CN108949635B (en) | Bacillus mucilaginosus, microbial fertilizer, preparation method and application thereof | |
| CN111763127A (en) | Preparation method for producing fresh earthworm full-nutrient fermented liquid fertilizer in large scale | |
| CN116535262A (en) | Drought-resistant organic fertilizer and preparation method thereof | |
| CN116354766A (en) | Carbon-based bio-organic fertilizer for promoting vegetable growth and improving soil and preparation method thereof | |
| CN108299097A (en) | A kind of preparation method of nitrogenous fertilizer | |
| WO2022222136A1 (en) | Environment-friendly seedling culturing fertilizer suitable for flowering plants, and production process therefor | |
| CN117165452B (en) | Soil conditioner and application thereof | |
| CN118303287B (en) | Method for planting third-generation lily bulbs by soil improvement and biological fertilizer used by same | |
| CN113943193B (en) | Marine biological fertilizer and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20201113 |