CN111793528A - Antibacterial phosphorus-free cleaning agent and preparation method and application thereof - Google Patents
Antibacterial phosphorus-free cleaning agent and preparation method and application thereof Download PDFInfo
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- 239000012459 cleaning agent Substances 0.000 title claims abstract description 58
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 57
- 238000002360 preparation method Methods 0.000 title abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 25
- IGFHQQFPSIBGKE-UHFFFAOYSA-N Nonylphenol Natural products CCCCCCCCCC1=CC=C(O)C=C1 IGFHQQFPSIBGKE-UHFFFAOYSA-N 0.000 claims abstract description 24
- SNQQPOLDUKLAAF-UHFFFAOYSA-N nonylphenol Chemical compound CCCCCCCCCC1=CC=CC=C1O SNQQPOLDUKLAAF-UHFFFAOYSA-N 0.000 claims abstract description 24
- 229940051841 polyoxyethylene ether Drugs 0.000 claims abstract description 24
- 229920000056 polyoxyethylene ether Polymers 0.000 claims abstract description 24
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 23
- HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000008367 deionised water Substances 0.000 claims abstract description 16
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 16
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000004327 boric acid Substances 0.000 claims abstract description 14
- 239000011734 sodium Substances 0.000 claims abstract description 14
- 229910052708 sodium Inorganic materials 0.000 claims abstract description 14
- BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 claims abstract description 14
- 239000000243 solution Substances 0.000 claims description 29
- 238000003756 stirring Methods 0.000 claims description 9
- 238000001914 filtration Methods 0.000 claims description 6
- 239000011259 mixed solution Substances 0.000 claims description 6
- 230000003204 osmotic effect Effects 0.000 claims description 5
- 239000000020 Nitrocellulose Substances 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 229920001220 nitrocellulos Polymers 0.000 claims description 3
- 239000011148 porous material Substances 0.000 claims description 2
- 238000000034 method Methods 0.000 claims 2
- 238000012360 testing method Methods 0.000 abstract description 12
- 230000000694 effects Effects 0.000 abstract description 4
- 239000007788 liquid Substances 0.000 abstract description 4
- 239000000126 substance Substances 0.000 abstract description 3
- 238000004140 cleaning Methods 0.000 description 12
- 239000011550 stock solution Substances 0.000 description 7
- 235000019441 ethanol Nutrition 0.000 description 5
- 229910019142 PO4 Inorganic materials 0.000 description 4
- 230000000845 anti-microbial effect Effects 0.000 description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 4
- 239000010452 phosphate Substances 0.000 description 4
- 238000001514 detection method Methods 0.000 description 3
- 239000003599 detergent Substances 0.000 description 3
- 230000003287 optical effect Effects 0.000 description 3
- 238000013112 stability test Methods 0.000 description 3
- 239000004094 surface-active agent Substances 0.000 description 3
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 2
- FJWGYAHXMCUOOM-QHOUIDNNSA-N [(2s,3r,4s,5r,6r)-2-[(2r,3r,4s,5r,6s)-4,5-dinitrooxy-2-(nitrooxymethyl)-6-[(2r,3r,4s,5r,6s)-4,5,6-trinitrooxy-2-(nitrooxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-3,5-dinitrooxy-6-(nitrooxymethyl)oxan-4-yl] nitrate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O)O[C@H]1[C@@H]([C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@@H](CO[N+]([O-])=O)O1)O[N+]([O-])=O)CO[N+](=O)[O-])[C@@H]1[C@@H](CO[N+]([O-])=O)O[C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O FJWGYAHXMCUOOM-QHOUIDNNSA-N 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000006185 dispersion Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- -1 polyoxyethylene nonyl phenyl ether Polymers 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 239000013556 antirust agent Substances 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000009640 blood culture Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000005536 corrosion prevention Methods 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 230000002354 daily effect Effects 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 230000001804 emulsifying effect Effects 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000005187 foaming Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
- C11D1/66—Non-ionic compounds
- C11D1/825—Mixtures of compounds all of which are non-ionic
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/0005—Other compounding ingredients characterised by their effect
- C11D3/0073—Anticorrosion compositions
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/0005—Other compounding ingredients characterised by their effect
- C11D3/0094—High foaming compositions
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/02—Inorganic compounds ; Elemental compounds
- C11D3/04—Water-soluble compounds
- C11D3/042—Acids
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/20—Organic compounds containing oxygen
- C11D3/2003—Alcohols; Phenols
- C11D3/2006—Monohydric alcohols
- C11D3/201—Monohydric alcohols linear
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/26—Organic compounds containing nitrogen
- C11D3/30—Amines; Substituted amines ; Quaternized amines
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/34—Organic compounds containing sulfur
- C11D3/3418—Toluene -, xylene -, cumene -, benzene - or naphthalene sulfonates or sulfates
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/48—Medical, disinfecting agents, disinfecting, antibacterial, germicidal or antimicrobial compositions
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
- C11D1/66—Non-ionic compounds
- C11D1/72—Ethers of polyoxyalkylene glycols
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Emergency Medicine (AREA)
- Inorganic Chemistry (AREA)
- Detergent Compositions (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides an antibacterial phosphorus-free cleaning agent and a preparation method and application thereof, wherein each 1L of the antibacterial phosphorus-free cleaning agent comprises the following components: nonylphenol polyoxyethylene ether TX-710-20 ml, nonylphenol polyoxyethylene ether TX-1220-45 ml, monoethanolamine 10-17ml, boric acid 2-4g, absolute ethyl alcohol 10-30ml, toluene-4-sodium sulfonate 1-2g and the balance of deionized water. The antibacterial phosphorus-free cleaning agent is suitable for a full-automatic biochemical analyzer, is colorless and transparent liquid in appearance, is stable in chemical property, and reduces the use cost while ensuring the test effect.
Description
Technical Field
The invention relates to the technical field of cleaning agents, in particular to an antibacterial phosphorus-free cleaning agent and a preparation method and application thereof.
Background
The full-automatic biochemical analyzer is an indispensable instrument and equipment for hospital clinical laboratory due to simple operation, reliable result and stable performance. The antibacterial phosphorus-free cleaning agent is also a necessity for daily operation of instruments, the consumption is high, and the antibacterial phosphorus-free cleaning agent in China mostly depends on import and is expensive, so that the medical cost is high. For example, Hitachi 7100, which is one of the most imported full-automatic biochemical analyzers, has the advantages of high detection speed, simple and convenient operation, good system repeatability, high accuracy and the like, the cleaning agent mainly adopts HITERGENT stock solution, HITERGENT stock solution is an imported cleaning agent, the price is higher, and although a small amount of domestic antibacterial phosphorus-free cleaning agent exists in the market at present, the actual effect is not ideal, and the requirements of customers cannot be met.
CN107881021A discloses a constant temperature bath cleaning solution and a preparation method thereof, wherein a bactericide of the constant temperature bath cleaning solution is Proclin 300, although Proclin 300 has low biological toxicity, the price is high, a surfactant is single TX-10, TX-10 only has single emulsifying capacity, and TX-10 is more in addition amount than TX-7, more viscous and not easy to weigh, triethanolamine basically crystallizes except in summer, is not easy to weigh, causes inconvenience for preparation of the cleaning solution, and is not beneficial to maintaining stability of cleaning solution performance.
Disclosure of Invention
The invention provides an antibacterial phosphorus-free cleaning agent, a preparation method and application thereof, which are suitable for a full-automatic biochemical analyzer.
The technical scheme of the invention is realized as follows: an antibacterial phosphorus-free cleaning agent, wherein each 1L of the antibacterial phosphorus-free cleaning agent contains: nonylphenol polyoxyethylene ether TX-710-20 ml, nonylphenol polyoxyethylene ether TX-1220-45 ml, monoethanolamine 10-17ml, boric acid 2-4g, absolute ethyl alcohol 10-30ml, toluene-4-sodium sulfonate 1-2g and the balance of deionized water.
Furthermore, each 1L of the antibacterial phosphorus-free cleaning agent contains: nonylphenol polyoxyethylene ether TX-715ml, nonylphenol polyoxyethylene ether TX-1230 ml, monoethanolamine 14ml, boric acid 3g, ethanol 20ml, toluene-4-sodium sulfonate 1.5g, and the balance of deionized water.
A preparation method of an antibacterial phosphorus-free cleaning agent comprises the following steps:
(1) adding monoethanolamine into deionized water to obtain a solution A, dissolving boric acid into absolute ethyl alcohol, uniformly mixing to obtain a solution B, adding the solution B into the solution A, and uniformly stirring to obtain a premixed solution;
(2) adding toluene-4-sodium sulfonate into the premixed solution obtained in the step (1), and uniformly stirring to obtain a mixed solution;
(3) and (3) adding nonylphenol polyoxyethylene ether TX-7 and nonylphenol polyoxyethylene ether TX-12 into the mixed solution obtained in the step (2), adding deionized water to a constant volume of 1L, uniformly stirring, and filtering to obtain the antibacterial phosphorus-free cleaning agent.
Further, in the step (2), a cellulose nitrate filter membrane with the pore size of 0.22um is adopted for filtration.
Further, the pH value of the antibacterial phosphorus-free cleaning agent is 10-10.5.
Further, the conductivity of the antibacterial phosphorus-free cleaning agent is 2-5 ms/cm.
Further, the osmotic pressure of the antibacterial non-phosphate cleaning agent is 600-800m0 sm/kg.
An application of antibacterial non-phosphorus detergent in full-automatic biochemical analyzer.
The invention has the beneficial effects that:
the antibacterial phosphorus-free cleaning agent is suitable for a full-automatic biochemical analyzer, is colorless and transparent liquid in appearance, has stable chemical properties, reduces the use cost while ensuring the test effect, saves the medical expenditure of people, is easy to popularize clinically, and has obvious social and economic benefits.
1. The invention adopts the toluene-4-sodium sulfonate as the solubilizer, reduces the viscosity and the turbidity of the cleaning agent, and has lower influence on the fluidity and the transparency of water after being added into the water bath.
2. The surfactant disclosed by the invention is a combination of TX-7 and TX-12, wherein TX-7 has excellent emulsification cleaning performance and stronger cleaning capability than other surfactants, but is diffused in water, and in addition, TX-12 is added to increase the water solubility of TX-7, and meanwhile, TX-12 is also a heavy-duty low-foaming lotion and can reduce the water hardness and increase the defoaming capability.
3. The monoethanolamine serving as the antirust agent can protect the sample sucking needle while washing the needle, and can provide an alkaline environment to wash off substances such as protein and the like on the sample sucking needle so as to prevent cross contamination; in addition, the monoethanolamine is liquid in most cases, the weighing is easy, and the monoethanolamine with the same concentration is dissolved in water, so that the PH value of the monoethanolamine is higher than that of triethanolamine, and the monoethanolamine is more favorable for corrosion prevention.
4. The preservative adopts boric acid to replace the common antibacterial agent sodium azide, has low toxicity and safe use, avoids the damage to reagent production personnel and environment, and can achieve the test effect equivalent to that of the antibacterial phosphorus-free cleaning agent in Hitachi original factories, but the cost is greatly reduced and the preparation method is simple.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be obtained by a person skilled in the art without inventive effort based on the embodiments of the present invention, are within the scope of the present invention.
An antibacterial phosphorus-free cleaning agent, wherein each 1L of the antibacterial phosphorus-free cleaning agent contains: nonylphenol polyoxyethylene ether TX-710-20 ml, nonylphenol polyoxyethylene ether TX-1220-45 ml, monoethanolamine 10-17ml, boric acid 2-4g, absolute ethyl alcohol 10-30ml, toluene-4-sodium sulfonate 1-2g and the balance of deionized water. The pH value of the antibacterial phosphorus-free cleaning agent is 10-10.5. The conductivity of the antibacterial phosphorus-free cleaning agent is 2-5 ms/cm. The osmotic pressure of the antibacterial non-phosphate cleaning agent is 600-.
The specific embodiment is as follows:
example 1
An antibacterial phosphorus-free cleaning agent, wherein each 1L of the antibacterial phosphorus-free cleaning agent contains: nonylphenol polyoxyethylene ether TX-715ml, nonylphenol polyoxyethylene ether TX-1230 ml, monoethanolamine 14ml, boric acid 3g, ethanol 20ml, toluene-4-sodium sulfonate 1.5g, and the balance of deionized water.
The preparation method of the antibacterial phosphorus-free cleaning agent comprises the following steps:
(1) adding monoethanolamine into deionized water to obtain a solution A, dissolving boric acid into absolute ethyl alcohol, uniformly mixing to obtain a solution B, adding the solution B into the solution A, and uniformly stirring to obtain a premixed solution;
(2) adding toluene-4-sodium sulfonate into the premixed solution obtained in the step (1), and uniformly stirring to obtain a mixed solution;
(3) and (3) adding nonylphenol polyoxyethylene ether TX-7 and nonylphenol polyoxyethylene ether TX-12 into the mixed solution obtained in the step (2), adding deionized water to a constant volume of 1L, uniformly stirring, filtering to obtain the antibacterial phosphorus-free cleaning agent, and filtering by using a cellulose nitrate filter membrane with the aperture of 0.22 um.
A Hitachi 7100 full-automatic biochemical analyzer is taken as an experimental object, and Hitachi 7100 original cleaning solution is HITERGENT original solution.
The following tests were carried out for example 1:
1. experiment of bacterial culture
3ml of solution is sucked from the reaction tank and added into a blood culture medium for culture before the biochemical analyzer is started up for three consecutive days, 0.1ml of solution is sucked from the culture medium every day and is transferred into a blood plate once, and whether bacteria grow or not is observed after the culture is carried out for three days.
And (3) testing results: after three days of culture no bacterial growth occurred.
2. Defoaming and cleaning Capacity test
Taking out the reaction cup after the power-on self-test is passed, observing whether bubbles are generated in the reaction tank, and observing whether bubbles are generated after the reaction tank is repeatedly changed with water; the old reaction cups (difference between cups is more than 800) are respectively cleaned by using HITERGENT stock solution and the antibacterial phosphorus-free cleaning agent described in the example one, and then the blank of the reaction cup is determined again.
And (3) testing results: and taking out the reaction cup after the Hitachi 7100 power-on self-test is passed, so that no bubbles are generated in the reaction tank, and after the reaction tank is repeatedly changed in water, no bubbles are generated.
The original HITERGENT solution and the antibacterial phosphorus-free cleaning agent described in the first example are respectively used for cleaning old reaction cups (the difference between cups is more than 800), the blank printing cup is printed after cleaning, the difference between cups is less than 200, and the two cleaning solutions have the same cleaning capability.
3. Optical system stability test
After starting the device, HITERGENT stock solution and the antibacterial phosphorus-free cleaning agent described in the embodiment are respectively used for changing water in the reaction tank, No. 1 cup is selected for measuring cup blanks for 20 times continuously, and the cup blank values of all wavelengths are statistically processed and compared.
The test results were as follows: comparing HITERGENT stock solutions with the antimicrobial non-phosphate cleaner of example one in terms of cup blank absorbance values at different wavelengths, it can be seen from Table 1: s is very close, CV values (all are less than 2 percent) accord with the specified range of the instrument, the precision is good, and the self-made antibacterial phosphorus-free cleaning agent has no influence on the detection result of the specimen.
TABLE 1 HITERGENT comparative results of stock solutions and antimicrobial non-phosphorus cleaners prepared in EXAMPLE I
Average, i.e., average of 20 cup blanks; s: standard deviation is the average of the distances of each data from the mean, i.e. 20 cup blanks from the meanAverage of the distances of (d); CV: the coefficient of variation represents the size of the dispersion of the blank of 20 cups, and the smaller the data, the smaller the dispersion.
4. EXAMPLE one test for determining the effective period of the antibacterial non-phosphate detergent
After 12 months, the pH, conductivity and osmotic pressure of the antimicrobial phosphorus-free detergent were measured and compared with the parameters at the completion of the formulation, followed by the optical system stability test.
The test results were as follows: after 12 months to the expiration date, the antibacterial phosphorus-free cleaning agent prepared in the first example is observed to be still colorless and transparent liquid without flocculent precipitates, and the pH, the osmotic pressure and the conductivity are detected to be 10.17 (10.25 at the beginning), 3.04ms/cm (3.11 ms/cm at the beginning) and 713m0sm/kg (720 m0sm/kg at the beginning); the results of the optical system stability test show (see table 2): s is very close, CV values (all are less than 2 percent) meet the specified range of the instrument, the precision is good, and the antibacterial phosphorus-free cleaning agent prepared in the embodiment I at the end of the expiration date has no influence on the detection result of the specimen.
TABLE 2 comparison of the end-of-term HITERGENT stock solutions and the antimicrobial phosphorus-free cleaners prepared in EXAMPLE I
Example 2
This embodiment is substantially the same as embodiment 1 except that: an antibacterial phosphorus-free cleaning agent, wherein each 1L of the antibacterial phosphorus-free cleaning agent contains: nonylphenol polyoxyethylene ether TX-710 ml, nonylphenol polyoxyethylene ether TX-1245 ml, monoethanolamine 17ml, boric acid 4g, ethanol 30ml, toluene-4-sodium sulfonate 2g, and the balance of deionized water.
Example 3
This embodiment is substantially the same as embodiment 1 except that: an antibacterial phosphorus-free cleaning agent, wherein each 1L of the antibacterial phosphorus-free cleaning agent contains: nonylphenol polyoxyethylene ether TX-720 ml, nonylphenol polyoxyethylene ether TX-1220 ml, monoethanolamine 10ml, boric acid 2g, ethanol 10ml, toluene-4-sodium sulfonate 1g, and the balance of deionized water.
Example 4
This embodiment is substantially the same as embodiment 1 except that: an antibacterial phosphorus-free cleaning agent, wherein each 1L of the antibacterial phosphorus-free cleaning agent contains: polyoxyethylene nonyl phenyl ether TX-713 ml, polyoxyethylene nonyl phenyl ether TX-1240 ml, monoethanolamine 12ml, boric acid 3g, ethanol 20ml, toluene-4-sodium sulfonate 1.5g, and the balance deionized water.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (8)
1. An antibacterial phosphorus-free cleaning agent, which is characterized in that each 1L of the antibacterial phosphorus-free cleaning agent contains: nonylphenol polyoxyethylene ether TX-710-20 ml, nonylphenol polyoxyethylene ether TX-1220-45 ml, monoethanolamine 10-17ml, boric acid 2-4g, absolute ethyl alcohol 10-30ml, toluene-4-sodium sulfonate 1-2g and the balance of deionized water.
2. The antibacterial phosphorus-free cleaning agent according to claim 1, wherein each 1L of antibacterial phosphorus-free cleaning agent comprises: nonylphenol polyoxyethylene ether TX-715ml, nonylphenol polyoxyethylene ether TX-1230 ml, monoethanolamine 14ml, boric acid 3g, absolute ethyl alcohol 20ml, toluene-4-sodium sulfonate 1.5g and the balance of deionized water.
3. A method for preparing the antibacterial phosphorus-free cleaning agent as defined in claim 1 or 2, which comprises the following steps:
(1) adding monoethanolamine into deionized water to obtain a solution A, dissolving boric acid into absolute ethyl alcohol, uniformly mixing to obtain a solution B, adding the solution B into the solution A, and uniformly stirring to obtain a premixed solution;
(2) adding toluene-4-sodium sulfonate into the premixed solution obtained in the step (1), and uniformly stirring to obtain a mixed solution;
(3) and (3) adding nonylphenol polyoxyethylene ether TX-7 and nonylphenol polyoxyethylene ether TX-12 into the mixed solution obtained in the step (2), adding deionized water to a constant volume of 1L, uniformly stirring, and filtering to obtain the antibacterial phosphorus-free cleaning agent.
4. The method according to claim 3, wherein the filtration in the step (2) is performed using a nitrocellulose filter having a pore size of 0.22 um.
5. The antibacterial phosphorus-free cleaning agent as claimed in claim 1 or 2, wherein the pH of the antibacterial phosphorus-free cleaning agent is 10-10.5.
6. The antibacterial phosphorus-free cleaning agent as claimed in claim 1 or 2, wherein the conductivity of the antibacterial phosphorus-free cleaning agent is 2-5 ms/cm.
7. The antibacterial phosphorus-free cleaning agent as claimed in claim 1 or 2, wherein the osmotic pressure of the antibacterial phosphorus-free cleaning agent is 600-800m0 sm/kg.
8. The use of the antibacterial phosphorus-free cleaning agent as defined in claim 1 or 2, which is used in a full-automatic biochemical analyzer.
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