CN111763722A - Method for identifying generation of PGCs mediated offspring through genome sequencing - Google Patents
Method for identifying generation of PGCs mediated offspring through genome sequencing Download PDFInfo
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Abstract
A method for identifying generation of PGCs mediated offspring by genome sequencing belongs to the field of biotechnology. The research method for identifying the generation of the PGCs mediated offspring through genome sequencing is practical, reliable, rigorous and accurate, has obvious effect, and provides a novel, feasible and efficient experimental method for identifying offspring chickens generated by the PGC-like cell mediated transformation formed by CEF.
Description
Technical Field
The invention relates to a method for identifying generation of PGCs mediated offspring through genome sequencing, belonging to the technical field of biology.
Background
The PGCs transplantation technology is an effective way to achieve species recovery by germ cell preservation, and ChumaS and others transplant PGCs of mice into and out of the testis of the mice after birth to differentiate the transplanted PGCs into spermatogonial stem cells and obtain offspring. In birds, due to the characteristic that PGCs can migrate through blood, Tagamit and the like successfully obtain offspring after injecting PGCs of white-feather chickens to receptors, successfully recovers recessive white-feather chickens, and Luyanqing and the like successfully realize PGCs transplantation of Donglan black-bone chickens and white-feather chickens in the white-feather chickens through the same method, and produces pure black-feather chickens.
Due to the development potential of germ cells, PGCs can be used as germplasm resources for protection and utilization, and can also be used as target cells for gene editing to produce gene-edited animals. Because the number of the isolated primary PGCs is small and the early embryo needs to be sacrificed, the application of the PGCs in the interspecific transplantation is not wide, and the conservation of the germ plasm resources of the poultry still faces a great problem. However, researches find that inducing pluripotent stem cells iPS can induce PGCs under specific conditions, does not have great harm to individuals, and can reprogram poultry somatic cells obtained in a large amount in a short period into iPS and further induce PGCs, which is undoubtedly the best way to solve the problem.
In the earlier stage of the subject group, a transdifferentiation optimization system for inducing chicken iPS by CEF and further inducing and differentiating into PGC-like cells is established, and meanwhile, a primary PGCs migration homing model is also established and offspring is successfully generated. However, whether the PGC-like cells formed by CEF transdifferentiation have the same ability of producing offspring as primary PGCs is still unclear, and the accuracy of in vitro induction is still uncertain, so that genome sequencing analysis of offspring chickens generated by the PGC-like cells formed by in vitro induced transdifferentiation mediated is urgently needed, feasibility and accuracy of offspring generation generated by in vitro induced PGCs are researched, and a foundation is laid for realizing wide application of PGCs in the poultry resource protection process.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a method for identifying PGCs-mediated generation of offspring through genome sequencing.
The technical scheme of the invention is as follows:
the invention provides a novel method for identifying generation of PGCs mediated offspring through genome sequencing: injecting PGC-like cells of Langshan chickens formed by induced transdifferentiation into white feather receptor chick embryo blood vessels incubated for 2.5 days at the early stage, sealing the vessels, then continuously incubating until the eggs are shelled, recording the phenotype properties of feather color, wing root color, shank color and the like of offspring chickens, extracting offspring chicken blood from PGC-like cell sources to perform genome sequencing, and analyzing the sources of different offspring by taking normal recessive white feather and Langshan chickens as controls. EGFP was analyzed for insertion in offspring chickens, along with similarities and dissimilarities in SNP distribution in the genomes of individuals with similar phenotypes in the offspring and differences in random insertion sites in the genomes. The result shows that the positive offspring can be definitely identified, and the positive offspring is derived from PGC-like formed by CEF induced transdifferentiation of the Langshan chicken.
The research method for identifying the generation of the PGCs mediated offspring through genome sequencing is practical, reliable, rigorous and accurate, has obvious effect, and provides a novel, feasible and efficient experimental method for identifying offspring chickens generated by the PGC-like cell mediated transformation formed by CEF.
The invention has the following beneficial effects:
the invention is precise, accurate, practical and widely applicable. PGCs can be used as germ plasm resources for protection and utilization due to the development potential of germ cells of the PGCs, can also be used as target cells for gene editing to produce gene editing animals, and can be used for generating offspring through allogenic/xenogenic transplantation of PGC-like cells formed by reprogramming avian somatic cells into iPS and further inducing, thereby breaking through the obstacles that the number of separated primary PGCs is small and early embryos need to be sacrificed. And genome sequencing analysis is carried out on the offspring chicken generated by the PGC-like cell mediation, and the method has great significance for researching the feasibility and the accuracy of the production of the offspring chicken mediated by the PGCs induced in vitro.
In addition, chickens are one of the most important model organisms in the study of genetics in non-mammals. Therefore, the experimental method disclosed by the invention is feasible in the germ plasm resource protection process of poultry and can be applied to other research fields of vertebrates. The invention links genome sequencing with identification of offspring chicken from PGCs, provides more theoretical basis and technical support for production research of PGC-like cell mediated offspring chicken formed by CEF transdifferentiation, is also beneficial to protection and utilization of bird germplasm resources, and is also beneficial to production research of transgenic chicken.
Detailed Description
PGC-like cell transplantation to induce transdifferentiation to produce progeny
Injecting PGC-like cells of Langshan chickens formed by induced transdifferentiation into a white feather receptor chick embryo blood vessel which is incubated for 2.5 days, continuously incubating until the chick is hatched after sealing, recording the phenotype of offspring chickens which are hatched, observing the phenotype change of the feather color and the like of the offspring chickens during brooding, weighing the offspring chickens every week, feeding the offspring chickens to 1 month for blood sampling, and performing genome sequencing.
Prove that the blot of the parent generation appears at the same time in the later generation
The method comprises the steps of collecting blood of wing veins of existing Langshan chicken flocks and recessive white-feather chicken flocks, extracting genome DNA, and completing genome sequencing under the charge of Yongzhou YouDiao organism company. Appropriate SSRs were screened in the GenBank database and germline transmission of parental SSRs in progeny was demonstrated by capillary sequencing (Sanger sequencing).
Secondly, designing a specific primer of the microsatellite fingerprint, amplifying the genome DNA of the existing Langshan chicken group and recessive white feather chicken group, and carrying out microsatellite detection by the Scutellaria organism company.
Correlation analysis between generations after similar feather color
And (3) carrying out wing vein blood sampling on individuals with similar feather colors in the offspring chicken, extracting genome DNA (deoxyribonucleic acid) for sequencing, and analyzing the genetic relationship among the individuals with similar feather colors through sequencing data.
Variation analysis of genes related to feather color among individuals with different feather colors
Through genome sequencing, the system screens the variation of feather color related genes melanin cortex Receptor1 (Melanocortin Receptor1, MC 1R), Tyrosinase (TYR), rat-related protein (AGRP) and the like, analyzes the genetic relationship among individuals with different feather colors through sequencing data, and analyzes whether different feather color characters occur due to gene separation or insertion of a lentivirus vector.
Claims (5)
1. A method for identifying PGCs-mediated offspring generation by genome sequencing is characterized in that Langshan chicken PGC-like cells formed by induced transdifferentiation are injected into white feather receptor chick embryo blood vessels which are hatched for 2.5 days at the early stage, the Langshan chicken is continuously hatched to be shelled after being sealed, the phenotype characters of offspring chicken are recorded, and offspring chicken blood from PGC-like cells is extracted for genome sequencing; taking normal recessive white feather and Langshan chicken as a reference, and analyzing the sources of different offspring; analyzing the insertion situation of EGFP in the offspring chicken, and simultaneously analyzing the difference of SNP distribution and the difference of random insertion sites of genomes in the genomes of individuals with similar phenotypes in the offspring; the result shows that the positive offspring can be definitely identified, and the positive offspring is derived from PGC-like formed by CEF induced transdifferentiation of the Langshan chicken.
2. The method for genome sequencing and identifying PGCs-mediated generation of offspring according to claim 1, comprising the steps of:
1) transplanting PGC-like cells formed by inducing transdifferentiation by CEF to generate offspring;
2) prove that the print of the parental generation appears at the same time in the next generation;
3) correlation analysis among generations after the similar feather colors;
4) and (3) analyzing variation of the feather color related genes among individuals with different feather colors.
3. The method for genome sequencing and identifying PGCs-mediated offspring generation according to claim 2, wherein the step 2) comprises:
wing vein blood sampling is carried out on the existing Langshan chicken flocks and recessive white feather chicken flocks, genome DNA is extracted, and genome sequencing is carried out; screening proper SSRs in a GenBank database, and proving that germ line transmission of the parent SSRs occurs in offspring through capillary sequencing;
secondly, designing a specific primer of the microsatellite fingerprint to amplify the genome DNA of the existing Langshan chicken flocks and recessive white feather chicken flocks.
4. The method for genome sequencing and identifying PGCs-mediated generation of offspring according to claim 3, wherein in step 3), the individuals with similar feather color in the offspring chicken are subjected to wing vein blood sampling, genome DNA is extracted for sequencing, and the genetic relationship between the individuals with similar feather color is analyzed through sequencing data.
5. The method of claim 4, wherein in step 4), genomic sequencing is used to systematically screen for variations in melanocortin receptor1, tyrosinase and acarus-related protein, which are genes associated with feather color, and sequencing data is used to analyze the genetic relationship between individuals with different feather colors, and to analyze whether the individuals with different feather colors have different feather color traits due to gene segregation or insertion of lentiviral vectors.
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6156569A (en) * | 1997-08-04 | 2000-12-05 | University Of Massachusetts Office Of Vice Chancellor For Research At Amherst | Prolonged culturing of avian primordial germ cells (PGCs) using specific growth factors, use thereof to produce chimeric avians |
| CN102212535A (en) * | 2011-04-08 | 2011-10-12 | 单云峰 | Construction and identification of over-expression lentivirus vector of rat GSK-3 beta (Glycogen Synthase Kinase-3 beta) target gene |
| AU2012201824A1 (en) * | 2005-02-01 | 2012-04-19 | Synageva Biopharma Corp. | Transgenic chickens |
| US20140363467A1 (en) * | 2011-06-10 | 2014-12-11 | University Of Georgia Research Foundation, Inc. | Avian induced pluripotent stem cells and their use |
| CN108289436A (en) * | 2015-09-01 | 2018-07-17 | 重组股份有限公司 | Identify the existing method of foreign allele in desired haplotype |
| CN109295238A (en) * | 2018-11-01 | 2019-02-01 | 江苏省家禽科学研究所 | The screening technique of blue-shelled egg layer genome SNP marker and its application |
| CN110122411A (en) * | 2019-05-13 | 2019-08-16 | 江苏省家禽科学研究所 | The construction method of one breeder inbred strais |
| CN110283894A (en) * | 2019-06-26 | 2019-09-27 | 扬州大学 | It is a kind of to identify the identification method that PGC transplants offspring chicken by plumage color binding molecule genetic marker |
-
2020
- 2020-07-27 CN CN202010731512.0A patent/CN111763722A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6156569A (en) * | 1997-08-04 | 2000-12-05 | University Of Massachusetts Office Of Vice Chancellor For Research At Amherst | Prolonged culturing of avian primordial germ cells (PGCs) using specific growth factors, use thereof to produce chimeric avians |
| AU2012201824A1 (en) * | 2005-02-01 | 2012-04-19 | Synageva Biopharma Corp. | Transgenic chickens |
| CN102212535A (en) * | 2011-04-08 | 2011-10-12 | 单云峰 | Construction and identification of over-expression lentivirus vector of rat GSK-3 beta (Glycogen Synthase Kinase-3 beta) target gene |
| US20140363467A1 (en) * | 2011-06-10 | 2014-12-11 | University Of Georgia Research Foundation, Inc. | Avian induced pluripotent stem cells and their use |
| CN108289436A (en) * | 2015-09-01 | 2018-07-17 | 重组股份有限公司 | Identify the existing method of foreign allele in desired haplotype |
| CN109295238A (en) * | 2018-11-01 | 2019-02-01 | 江苏省家禽科学研究所 | The screening technique of blue-shelled egg layer genome SNP marker and its application |
| CN110122411A (en) * | 2019-05-13 | 2019-08-16 | 江苏省家禽科学研究所 | The construction method of one breeder inbred strais |
| CN110283894A (en) * | 2019-06-26 | 2019-09-27 | 扬州大学 | It is a kind of to identify the identification method that PGC transplants offspring chicken by plumage color binding molecule genetic marker |
Non-Patent Citations (2)
| Title |
|---|
| HYUN-JUN JANG等: "Gene Expression and DNA Methylation Status of Chicken Primordial Germ Cells", 《MOL BIOTECHNOL》 * |
| 赵瑞丰: "鸡CEF重编程成为PGC诱导体系的建立及其迁移归巢功能的研究", 《中国优秀硕士学位论文全文数据库农业科技辑》 * |
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