CN111592984A - Multifunctional cell detector - Google Patents
Multifunctional cell detector Download PDFInfo
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- CN111592984A CN111592984A CN202010549260.XA CN202010549260A CN111592984A CN 111592984 A CN111592984 A CN 111592984A CN 202010549260 A CN202010549260 A CN 202010549260A CN 111592984 A CN111592984 A CN 111592984A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/52—Mobile; Means for transporting the apparatus
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M39/00—Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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Abstract
The invention discloses a multifunctional cell detector, which comprises a base, wherein a rotating shaft is axially connected on the top surface of the base, a rotating disc is sleeved on the rotating shaft, the top end of the rotating shaft is axially connected with an output shaft of a driving motor, the top end of the driving motor is provided with an electric telescopic rod, one end of an inner rod of the electric telescopic rod is provided with a first connecting rod, a second connecting rod is fixed between the inner rod of the electric telescopic rod and the first connecting rod, the top end of the first connecting rod is provided with a display screen, one side of the display screen is provided with a data processor, the bottom end of the first connecting rod is provided with a camera, a plurality of object stages are circumferentially distributed on the rotating disc, and the outer sides of the object stages are, living cells such as insects and the like provide an optimal living environment, the types of the detected cells are improved, the cells are prevented from dying in the detection process, the detection efficiency is further improved, and the production cost is reduced.
Description
Technical Field
The invention relates to the technical field of biomedical equipment, in particular to a multifunctional cell detector.
Background
The cell culture medium is not only a basic substance for supplying cell nutrition and promoting cell reproduction and proliferation in the cultured cells, but also a living environment for the growth and the proliferation of the cultured cells, the composition framework is the components which are necessary for the cell composition such as amino acid, vitamin, carbohydrate, inorganic ion and the like and participate in the cell growth and metabolism, and when the cell culture medium is used for culturing different types of cells, the required growth temperature is different, for example, the optimal adaptation temperature of mammalian cells is 36-37 ℃, the optimal adaptation temperature of insect cells is about 27 ℃, a cell detector is needed to observe the growth condition and growth rate of the cells, however, the current cell detector can only detect one cell type, the detection efficiency is influenced, and the external temperature can not be ensured to be at the survival temperature of the cells in the detection process, so that the problem of partial cell death in the detection process can be caused.
Disclosure of Invention
The invention aims to provide a multifunctional cell detector, which solves the problems in the prior art, can detect more cell types, can provide optimal production conditions and improves the detection efficiency.
In order to achieve the purpose, the invention provides the following scheme: the invention provides a multifunctional cell detector, which comprises a base, wherein a rotating shaft is axially connected to the top surface of the base, a rotating disc is sleeved on the rotating shaft, an output shaft of a driving motor is axially connected to the top end of the rotating shaft, an electric telescopic rod is arranged at the top end of the driving motor, a first connecting rod is arranged at one end of an inner rod of the electric telescopic rod, a second connecting rod is fixed between the inner rod and the first connecting rod of the electric telescopic rod, a display screen is arranged at the top end of the first connecting rod, a data processor is arranged on one side of the display screen, a camera is arranged at the bottom end of the first connecting rod and is in signal connection with the data processor, the camera is in signal connection with the display screen, the data processor is in signal connection with the display screen, a plurality of object carrying tables are circumferentially distributed on the rotating disc, a constant temperature mechanism is sleeved on the outer sides of the object carrying tables and comprises, two layers of insulating layers are sleeved outside the heat storage and insulation layer, electric heating wires are sleeved between the insulating layers, the insulating layer is sleeved outside the insulating layer on the outermost side, the base is provided with an aseptic water tank, the aseptic water tank is connected with the objective table through a pipeline, and a microporous filter membrane is arranged in the aseptic water tank.
Preferably, a water pump is arranged in the sterile water tank, the top end of the water pump is connected with a water inlet pipe, and a stop valve is arranged on the water inlet pipe.
Preferably, two identical first through holes are formed in the rotary table, a pipeline connecting piece is arranged at the bottom end of each first through hole, and the pipeline connecting piece is detachably connected with the water inlet pipe.
Preferably, a water outlet pipe is arranged in the sterile water tank, a stop valve which is the same as that on the water inlet pipe is arranged on the water outlet pipe, and the water outlet pipe is detachably connected with the pipeline connecting piece.
Preferably, a groove is formed in the objective table, a containing box is placed in the groove, two second through holes are formed in the bottom wall of the groove, and the first through holes are communicated with the second through holes.
Preferably, a motor protective housing is arranged between the rotating shaft and the electric telescopic rod, a fixing frame is arranged on the inner wall of the motor protective housing, the driving motor is fixed on the fixing frame, a shaft sleeve is fixed at the bottom end of the motor protective housing, the rotating shaft is fixedly connected with the shaft sleeve, and the electric telescopic rod is fixed at the top end of the motor protective housing.
Preferably, the heat insulation layer is a stainless steel partition plate or a plastic partition plate.
Preferably, the heat storage and insulation layer comprises two heat insulation layers and a heat storage medium layer embedded between the two heat insulation layers, the heat insulation layer is made of polyurethane foam, and the heat storage medium layer is made of shaped paraffin pellets.
The invention discloses the following technical effects: firstly, a constant temperature mechanism is sleeved outside each objective table, then a heating wire is started, the temperature in the objective tables can be controlled at will and kept constant, living cells of various biological species are ensured to be in the optimal growth state, at the moment, cell culture media are respectively placed on each objective table, a driving motor is started to drive a turntable to rotate, one objective table is stopped after being correspondingly arranged under a camera, image information in the objective tables is collected through the camera and is transferred into a display screen for imaging, a user can conveniently and directly observe the cell growth condition, the image data collected by the camera is transmitted into a data processor for processing, after the work is stopped, sterile water in a sterile water tank is circularly led into the objective tables and is filtered through a microporous filter membrane, the multifunctional cell detector is convenient to use for the next time, and the multifunctional cell detector can provide the optimal living environment for the living cells of people, mammals, insects and the like, therefore, the optimal growth rate and the optimal growth condition of the cell are detected, the variety of the detected cell is improved, the cell is prevented from dying in the detection process, the detection efficiency is further improved, and the production cost is reduced.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without inventive exercise.
FIG. 1 is a schematic structural view of the multi-functional cell detector of the present invention;
FIG. 2 is an enlarged view of a portion of FIG. 1;
wherein, 1 is the base, 2 is the pivot, 3 is the carousel, 4 is driving motor, 5 is electric telescopic handle, 6 is the head rod, 7 is the second connecting rod, 8 is the display screen, 9 is data processor, 10 is the camera, 11 is the objective table, 12 is the heat accumulation heat preservation, 12.1 is the heat preservation, 12.2 is the heat accumulation dielectric layer, 13 is the insulating layer, 14 is electric heating wire, 15 is the insulating layer, 16 is aseptic water tank, 17 is the water pump, 18 is the inlet tube, 19 is the stop valve, 20 is first through-hole, 21 is the pipe connection spare, 22 is the outlet pipe, 23 is the recess, 24 is for holding the box, 25 is the second through-hole, 26 is the motor protective housing, 27 is the mount, 28 is the axle sleeve.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in further detail below.
Referring to fig. 1-2, the present invention provides a multifunctional cell detector, including a base 1, a rotating shaft 2 is coupled to a top surface of the base 1, a rotating disc 3 is sleeved on the rotating shaft 2, an output shaft of a driving motor 4 is coupled to a top end of the rotating shaft 2, a type of the driving motor 8 is preferably an ac motor of Y100L2-4, parameters are 3kw, 1500rpm, and v1380v, an electric telescopic rod 5 is disposed at a top end of the driving motor 4, a first connecting rod 6 is disposed at one end of an inner rod of the electric telescopic rod 5, a second connecting rod 7 is fixed between the inner rod of the electric telescopic rod 5 and the first connecting rod 6, a display screen 8 is disposed at a top end of the first connecting rod 6, a data processor 9 is disposed at one side of the display screen 8, the display screen 8 and the data processor 9 are related art, and no further description is given here, and a camera 10 is disposed at a bottom, the up-and-down telescopic movement of the inner rod of the electric telescopic rod 5 drives the camera 10 to adjust the height from the object stage 11, the camera 10 is in signal connection with the data processor 9, the camera 10 is in signal connection with the display screen 8, the data processor 9 is in signal connection with the display screen 8, one side of the camera 10 is also provided with a light path system (not shown in the figure), the light path system is a ccd imaging special light path system, which is not repeated in the prior art, the light path system provides a light source to ensure sufficient light, cells can be imaged on the camera 10, the whole mechanism formed by the camera 10 and the object stage 11 can be placed under the platform for observation, the camera 10 is preferably a high-definition BNC industrial camera, can enlarge 100 times for observation, ensures that the enlargement distance during the on-platform observation is enough for clearly observing the cells, a plurality of object stages 11 are circumferentially arranged on the turntable 3, and a temperature sensor (not shown in the figure) is arranged in the object stage, the temperature sensor is in signal connection with an electric heating wire 14, the temperature is adjusted by an external controller (not shown in the figure), the number of the object stages 11 is preferably 4, a constant temperature mechanism is sleeved on the outer side of the object stage 11, the constant temperature mechanism comprises a heat storage heat preservation layer 12 arranged on the innermost side, two insulation layers 13 are sleeved on the outer side of the heat storage heat preservation layer 12, the electric heating wire 14 is sleeved between the two insulation layers 13, a heat insulation layer 15 is sleeved on the outer side of the insulation layer 13 on the outermost side, the heat insulation layer 15 is a stainless steel partition plate or a plastic partition plate, the heat storage heat preservation layer 12 comprises two heat preservation layers 12.1 and a heat storage medium layer 12.2 embedded between the two heat preservation layers 12.1, the heat preservation layer 12.1 is polyurethane foam, the heat storage medium layer 12.2 is a shaped paraffin pill, a sterile water tank 16 is arranged on the base 1, the sterile water tank 16 is internally provided with a 0.44/0.22um microporous filter membrane.
Further optimize the scheme, be equipped with water pump 17 in the aseptic water tank 16, the top of water pump 17 is connected with inlet tube 18, be equipped with stop valve 19 on the inlet tube 18, two identical first through-holes 20 have been seted up on the carousel 3, the bottom of first through-hole 20 is equipped with pipe connection 21, pipe connection 21 can be dismantled with inlet tube 18 and be connected, be equipped with outlet pipe 22 in the aseptic water tank 16, be equipped with on the outlet pipe 22 with inlet tube 18 on the same stop valve 19, outlet pipe 22 can be dismantled with pipe connection 21 and be connected, seted up recess 23 on the objective table 11, place in the recess 23 and hold box 24, two second through-holes 25 have been seted up on the diapire of recess 23, first through-hole 20 link up with second through-hole 25 mutually.
Furthermore, in order to improve the transfer efficiency of switching the containing box 24, a motor protective housing 26 is arranged between the rotating shaft 2 and the electric telescopic rod 5, a fixing frame 27 is arranged on the inner wall of the motor protective housing 26, the driving motor 4 is fixed on the fixing frame 27, a shaft sleeve 28 is fixed at the bottom end of the motor protective housing 26, the rotating shaft 2 is fixedly connected with the shaft sleeve 28, and the electric telescopic rod 5 is fixed at the top end of the motor protective housing 26.
Firstly, the constant temperature mechanism is sleeved at the outer sides of 4 object stages 11, and the electric heating wire 14 is started aiming atFor example, in the case of mammalian cells, the present invention also requires the introduction of an existing gas control system (not shown) for detecting mammalian cells, typically ensuring 5% Co2When the temperature of the objective table 11 is raised to about 36 ℃, the temperature sensor transmits an electric signal to the external controller, the external controller stops the electric heating wire 14 and sends out an acoustic signal to prompt an experimenter to put the mammalian cell culture medium into the containing box 24, when the temperature is reduced, the temperature sensor detects and starts the electric heating wire 14 to be raised to 36 ℃ through the external controller, the cell is ensured to be always in the optimal growth environment, the driving motor 4 is started to drive the rotating shaft 2 to rotate according to the objective table 11 to be detected, the rotating disc 3 synchronously moves along with the rotating shaft 2 until the objective table 11 is positioned under the camera 10, then the extension height of the rod in the electric telescopic rod 5 is adjusted, so that the height of the camera 10 is adjusted to ensure that the objective table is in the optimal visual field condition, the image of the cell culture medium is collected through the camera 10 and imaged through the display screen 8, the image data of the cell growth is transmitted to the data processor 9, the optimal growth rate and growth condition of the cell can be conveniently detected, after the cell is used, the water inlet pipe 18 and the water outlet pipe 22 are respectively inserted into the two pipeline connecting pieces 21, the water pump 17 is started, the water pump 17 pumps the sterile water in the sterile water tank 16, the sterile water enters the groove 23 and the containing box 24 after passing through the water inlet pipe 18, the first through hole 20 and the second through hole 25, and circularly flows back to the sterile water tank 16 through the second through hole 25, the first through hole 20 and the water outlet pipe 22, and the cleaning work is completed by filtering through a microporous filter screen.
In the description of the present invention, it is to be understood that the terms "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, are merely for convenience of description of the present invention, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.
The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.
Claims (8)
1. A multifunctional cell detector is characterized in that: comprises a base (1), a rotating shaft (2) is connected to the top surface of the base (1) in a shaft coupling manner, a rotating disc (3) is connected to the rotating shaft (2) in a sleeved manner, an output shaft of a driving motor (4) is connected to the top end of the rotating shaft (2) in a shaft coupling manner, an electric telescopic rod (5) is arranged at the top end of the driving motor (4), a first connecting rod (6) is arranged at one end of an inner rod of the electric telescopic rod (5), a second connecting rod (7) is fixed between the inner rod of the electric telescopic rod (5) and the first connecting rod (6), a display screen (8) is arranged at the top end of the first connecting rod (6), a data processor (9) is arranged at one side of the display screen (8), a camera (10) is arranged at the bottom end of the first connecting rod (6), the camera (10) is in signal connection with the data processor (9), and the camera (, data processor (9) and display screen (8) signal connection, a plurality of objective table (11) have been laid to circumference on carousel (3), constant temperature mechanism has been cup jointed in the objective table (11) outside, constant temperature mechanism is including setting up at the heat accumulation heat preservation (12) of the most inboard, two-layer insulating layer (13) have been cup jointed to heat accumulation heat preservation (12) outside, and is two-layer electric heating wire (14) have been cup jointed between insulating layer (13), insulating layer (15) have been cup jointed in the outside of insulating layer (13) in the outside, be equipped with aseptic water tank (16) on base (1), aseptic water tank (16) are through pipe connection objective table (11), be equipped with millipore filtration in aseptic water tank (16).
2. The multi-functional cellular detector of claim 1, wherein: be equipped with water pump (17) in aseptic water tank (16), the top of water pump (17) is connected with inlet tube (18), be equipped with stop valve (19) on inlet tube (18).
3. The multi-functional cellular detector of claim 2, wherein: two identical first through holes (20) are formed in the rotary plate (3), a pipeline connecting piece (21) is arranged at the bottom end of each first through hole (20), and the pipeline connecting piece (21) is detachably connected with the water inlet pipe (18).
4. The multi-functional cellular detector of claim 3, wherein: be equipped with outlet pipe (22) in aseptic water tank (16), be equipped with on outlet pipe (22) with inlet tube (18) on same stop valve (19), outlet pipe (22) can be dismantled with pipe connection spare (21) and be connected.
5. The multi-functional cellular detector of claim 4, wherein: the novel multifunctional storage rack is characterized in that a groove (23) is formed in the objective table (11), a storage box (24) is placed in the groove (23), two second through holes (25) are formed in the bottom wall of the groove (23), and the first through holes (20) are communicated with the second through holes (25).
6. The multi-functional cellular detector of claim 1, wherein: be equipped with motor protective housing (26) between pivot (2) and electric telescopic handle (5), be equipped with mount (27) on the inner wall of motor protective housing (26), driving motor (4) are fixed on mount (27), motor protective housing (26) bottom mounting has axle sleeve (28), pivot (2) and axle sleeve (28) rigid coupling, electric telescopic handle (5) are fixed on the top of motor protective housing (26).
7. The multi-functional cellular detector of claim 1, wherein: the heat insulation layer (15) is a stainless steel partition plate or a plastic partition plate.
8. The multi-functional cellular detector of claim 1, wherein: the heat-storage insulating layer (12) comprises two insulating layers (12.1) and a heat-storage medium layer (12.2) embedded between the two insulating layers (12.1), the insulating layer (12.1) is made of polyurethane foam, and the heat-storage medium layer (12.2) is made of shaped paraffin pills.
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| CN202010549260.XA CN111592984A (en) | 2020-06-16 | 2020-06-16 | Multifunctional cell detector |
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| CN202010549260.XA CN111592984A (en) | 2020-06-16 | 2020-06-16 | Multifunctional cell detector |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115369019A (en) * | 2022-10-21 | 2022-11-22 | 江苏优仕生物科技发展有限公司 | Remote intelligent monitoring equipment for microbial strain production |
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| CN213388701U (en) * | 2020-08-25 | 2021-06-08 | 成都益科达生物科技有限公司 | Cell culture dish for biotechnology |
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| CN115369019A (en) * | 2022-10-21 | 2022-11-22 | 江苏优仕生物科技发展有限公司 | Remote intelligent monitoring equipment for microbial strain production |
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