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CN111567261B - Plant toner and application and use method thereof - Google Patents

Plant toner and application and use method thereof Download PDF

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Publication number
CN111567261B
CN111567261B CN202010409358.5A CN202010409358A CN111567261B CN 111567261 B CN111567261 B CN 111567261B CN 202010409358 A CN202010409358 A CN 202010409358A CN 111567261 B CN111567261 B CN 111567261B
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plant
toner
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lactobacillus rhamnosus
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CN111567261A (en
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江志阳
尹微
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Institute of Applied Ecology of CAS
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Institute of Applied Ecology of CAS
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05B—PHOSPHATIC FERTILISERS
    • C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
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    • C05F17/40—Treatment of liquids or slurries
    • C—CHEMISTRY; METALLURGY
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    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/50—Surfactants; Emulsifiers
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00—Fertilisers characterised by their form
    • C05G5/20—Liquid fertilisers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/225—Lactobacillus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Health & Medical Sciences (AREA)
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  • Biotechnology (AREA)
  • Zoology (AREA)
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  • Pest Control & Pesticides (AREA)
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  • Biodiversity & Conservation Biology (AREA)
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  • Forests & Forestry (AREA)
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  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Biomedical Technology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Fertilizers (AREA)

Abstract

The invention belongs to the technical field of plant toner, and particularly relates to a plant toner and an application and use method thereof. Comprises 7-9 parts of lactobacillus rhamnosus, 1-2 parts of calcium, 0.25 part of magnesium, 0.1 part of boron, 0.5 part of potassium and 0.15 part of ferrum according to parts by weight. When in use, the toner is diluted by 800-1000 times by water and is sprayed on the surface of the plant at different periods of plant growth. The invention has the advantages that: the product components only contain microorganisms and mineral elements, and do not contain any chemical pesticide, chemical composition, phytohormone and the like, so that the safety and ecological safety of agricultural products are ensured, and the effect of fruit color enhancement can be achieved.

Description

Plant toner and application and use method thereof
Technical Field
The invention belongs to the technical field of plant hyperchromic agents, and particularly relates to a plant hyperchromic agent, and an application and a use method thereof.
Background
As is well known, fruit swelling, sweetening, coloring can greatly improve the quality and the appearance of the fruits, flowers or vegetables; the fruit can be listed in advance by promoting precocity, and both are favorable for the plant to sell better price, thereby obviously improving the planting benefit. Therefore, coloring, sweetening and accelerating maturity are important rings of plant later-period management, and directly influence the planting benefit of the current year.
Taking fruits as an example, genetic factors, illumination, moisture, temperature and sugar accumulation of the fruits are main factors influencing fruit color increasing, and microorganisms, metabolites thereof and mineral nutrition are highly positively correlated with the factors, polysaccharide substances in the metabolites of the microorganisms are key substances for plant coloring, and mineral elements in the combined state of the metabolites of the microorganisms can promote the absorption rate of the mineral elements by the plants.
At present, research and application of fruit coloring are mainly focused on plant growth regulators such as ethephon and abscisic acid and multifunctional fertilizers. The fruit coloring agent is generally a fertilizer containing trace elements, major elements and biological materials, and the fruit coloring agent disclosed in patent CN102057953A contains photosynthetic bacteria, anthocyanin liquid, chitin, chitosan, potassium dihydrogen phosphate, potassium sulfate and wetting agent. The color enhancer contains photosynthetic bacteria, the nutrition of the bacteria is rich, the growth can be promoted, the accumulation of sugar is enhanced under the illumination condition, the conversion of reducing sugar is promoted, and the coloring of fruits is promoted.
In addition, studies show that sodium butyrate and brassinolide also have reports of promoting apple coloring. Although the above substances or fertilizers can play a role in color conversion, a large number of experimental researches show that: the phenomenon that the leaves of the grape trees fall, the fruits crack, the fruit grains fall off, the fruits cannot be stored enduringly and the like can be easily caused by singly using the ethephon preparation in the color conversion process of the grapes; abscisic acid is directly and quickly colored on fruits such as grapes and apples, but is not easy to control in practical use because the abscisic acid is sensitive to light, is unstable and easy to decompose in the use process and is easy to metabolize. Meanwhile, the release of the national GB/T37500-2019 'high performance liquid chromatography for measuring plant growth regulators in fertilizers' standard enables illegal behaviors of adding plant growth regulators in fertilizers to have technical support. Solves the problems that a plurality of fertilizers added with plant growth regulators exist in the current market, the market order is disturbed, and the crop safety is damaged. Plant growth regulators are a class of substances that have similar physiological and biological effects as plant hormones. Examples of compounds having the function of controlling plant growth and development include diethyl aminoethyl hexanoate (DA-6), forchlorfenuron, sodium nitrophenolate, gibberellin, ethephon, brassinolide and paclobutrazol. The use of plant growth regulators generally does not pose a hazard to human health. However, if the use is irregular, the crops can grow too fast, or the growth can be inhibited and even die. Has certain influence on the quality of agricultural products and is harmful to human health.
Therefore, the pure biological, safe and effective fruit colorant with good coloring effect has important practical significance.
Disclosure of Invention
In order to solve the technical problems, the invention provides a plant toner and an application and use method thereof.
The plant toner is prepared by mixing lactobacillus rhamnosus and various mineral substances, adding the mixture into a culture medium, and fermenting for a certain time under a certain condition.
Preferably, the food comprises 7-9 parts of lactobacillus rhamnosus, 1-2 parts of calcium, 0.25 part of magnesium, 0.1 part of boron, 0.5 part of potassium and 0.15 part of iron in parts by weight.
Further preferably, the formula of the culture medium is as follows: 1000mL contains 10-12g of peptone, 10-12g of beef extract, 5-8g of yeast powder, 20-23g of glucose, 2-3g of triammonium citrate, 0.058-0.06g of magnesium sulfate heptahydrate, 3.12-3.17g of sodium acetate, 1.63-1.71g of disodium hydrogen phosphate, 2.25-2.5g of potassium acetate, 801-90 mL of tween, 0.25-0.3g of manganese sulfate tetrahydrate and the balance of water.
Further preferably, the fermentation conditions are: fermenting at pH of 6.5-7.5 and temperature of 30-34 deg.C and ventilation ratio of 1:0.3-0.5 for 40-50 h.
Further preferably, the inoculation amount of the mixture formed by the lactobacillus rhamnosus and various minerals is 1-3% of the mass of the culture medium.
Further preferably, the plant is strawberry, apple, blueberry, cherry, kiwi fruit, banana, rose, Chinese rose, cabbage, tomato, eggplant.
The invention also provides the application of the plant toner as a fruit colorant.
The invention also provides the application of the plant toner as a plant conditioning fertilizer.
The invention also provides a use method of the plant toner, wherein the toner is diluted by water by 800-fold and 1000-fold, and is sprayed on the surface of a plant at different periods of plant growth.
Compared with the prior art, the invention has the advantages that:
1. the toner provided by the invention is a pure biological preparation, and completely replaces a chemical toner and a plant growth regulator, and the biological preparation can promote the coloring of fruits, vegetables and flowers, improve the quality of agricultural products and ensure the safety of the agricultural products;
2. the color enhancer provided by the invention is obtained by fermenting lactobacillus rhamnosus in a culture medium containing mineral elements, so that the lactobacillus rhamnosus can promote plant cell division under the action of the mineral elements and under a certain pH value system, and hydrogen is produced in the fermentation process, so that minerals form a certain chelated state, and the obtained chelated minerals are absorbed and accumulated by the plant cells under the plant cell division, thereby promoting the coloring of plant fruits; in addition, in the cell division metabolic process of the microorganism, the microorganism can ferment and accumulate to obtain a plurality of monosaccharides, and the monosaccharides and the formed chelated minerals which are easy to absorb enable plants to absorb and accumulate interaction under the action of microorganism division, so that the coloring of fruits is promoted, and the coloring of the fruits is more obvious due to organic combination of the two accumulated interactions.
3. The toner provided by the invention can improve the cell division of leaves and promote photosynthesis when being applied to crops, so that the product is rich in chelated minerals and various nutrient elements which are absorbed and accumulated by plants, thereby promoting the growth and the coloring of the plants, and meanwhile, the additionally added bacterial strain has the characteristics of acid resistance, bile salt resistance, multiple antibiotic resistance and the like.
4. Improve the quality of fruits and promote environmental protection. The natural microbial elements used in the toner provided by the invention can solve the problem of improving the quality of fruits and reduce the after-effect of the chemical plant growth regulator on the environment.
Detailed Description
The following examples are presented to further illustrate embodiments of the present invention, and it should be understood that the embodiments described herein are for purposes of illustration and explanation only and are not intended to limit the invention.
In the following examples, Lactobacillus rhamnosus (Lactobacillus rhamnosus) is a commercially available strain, and the calcium, magnesium, boron, potassium and iron are extracted from minerals; the extraction method can be according to the existing method; thus obtaining the mineral calcium, the mineral magnesium, the mineral boron, the mineral potassium and the mineral iron.
Examples 1,
The toner comprises the following components in parts by weight: 7 parts of lactobacillus rhamnosus, 2 parts of calcium, 0.25 part of magnesium, 0.1 part of boron, 0.5 part of potassium and 0.15 part of iron, and mixing to obtain a mixture; adding the mixture into culture medium, adjusting system pH to 6.5-7.5, fermenting at 30 deg.C and ventilation ratio of 1:0.3 (air flow rate is generally expressed by ventilation ratio in fermentation production, and air volume ratio is generally expressed by air volume ratio of culture solution per unit volume in one minute, and unit is V/V.m) for 50 hr to obtain toner; wherein the mixture accounts for 3% of the culture medium.
The components of the culture medium are as follows: the formula of 1L is 10g of peptone, 10g of beef extract, 5g of yeast powder, 20g of glucose, 2g of triammonium citrate, 0.058g of magnesium sulfate heptahydrate, 3.12g of sodium acetate, 1.63g of disodium hydrogen phosphate, 2.25g of potassium acetate, 801 mL of tween, 0.25g of manganese sulfate tetrahydrate and the balance of water.
Application example
Experiment set 2 treatments, 1 control, treatment 1: diluting the color enhancer fermentation liquor prepared in the above example by 1000 times with water, applying to an experimental group, treating 2: using seradipine as a control, blank: spraying clear water as a blank; the processed variety is apple.
Spraying once after the apple bears fruit, and spraying once in the fruit color-changing period; the spraying amount of each time of the experimental group is 500 g per mu of the toner stock solution, and other management is the same as conventional management. The experiment was repeated three times for a total of 27 apple trees.
When the fruits are ripe, sampling according to the fertilizer field test requirements, respectively measuring several indexes influencing coloring, and further calculating the coloring index, the content of soluble solids, the content of titratable acid and the content of Vc.
Wherein,
Figure BDA0002492607040000051
degree of coloration is obtained from the coloration index:
Figure BDA0002492607040000052
the content of soluble solids, the content of titratable acid and the content of Vc are respectively determined according to the content of the soluble solids, namely refractometer method GB/T12295-; GB/T12293-1990 determination of titratable acidity of fruit and vegetable products; GB/T6195-1986 fruit and vegetable vitamin C assay method.
After testing, the content of soluble solids, the content of titratable acid and the content of Vc in the apples are respectively 17.2 percent, 0.22 percent and 4.17mg/100g after the toner is adopted. The content of soluble solids of the S-abscisic acid, the content of titratable acid and the content of Vc are respectively 16.7 percent, 0.23 percent and 4.09mg/100 g. The content of the clear water reference soluble solid, the content of the titratable acid and the content of Vc are respectively 12.7 percent, 0.41 percent and 4.89mg/100 g.
200 apples were taken for each treatment, the color index of the experimental group was 81.11%, the color index of the abscisic acid was 77.13%, and the color index of the clear water control group was 62.12%. The results show that the product can meet the requirements in aspects of apple color enhancement and quality improvement.
Examples 2,
The toner comprises the following components in parts by weight: 8 parts of lactobacillus rhamnosus, 1 part of calcium, 0.25 part of magnesium, 0.1 part of boron, 0.5 part of potassium and 0.15 part of iron are mixed to obtain a mixture; adding the mixture into culture medium, fermenting at 32 deg.C and pH of 6.5-7.5 under aeration ratio of 1:0.4 for 45 hr. Wherein the mixture accounts for 2% of the culture medium.
The formula of the culture medium is as follows: the formula of 1L is 10g of peptone, 10g of beef extract, 5g of yeast powder, 20g of glucose, 2g of triammonium citrate, 0.058g of magnesium sulfate heptahydrate, 3.12g of sodium acetate, 1.63g of disodium hydrogen phosphate, 2.25g of potassium acetate, 801 mL of tween, 0.25g of manganese sulfate tetrahydrate and the balance of water.
The application comprises the following steps:
experiments are designed with 2 treatments and 1 control, and the apple is sprayed once after fruit setting and once in the fruit color-changing period. Treatment 1: the toner obtained in example 2 was diluted 900 times with water and sprayed onto the foliage. And (3) treatment 2: 0.5mg/L of abscisic acid; control, clear water. The experiment was repeated three times for a total of 27 apple trees.
The fertilizer spraying is based on a conventional foliar fertilizer spraying method, and other management is the same as conventional management.
Sampling is carried out according to the field test requirements of the fertilizer, and a plurality of indexes influencing coloring, the content of soluble solid matters, the content of titratable acid and the content of Vc are respectively determined, and the results show that the content of the soluble solid matters, the content of titratable acid and the content of Vc of the product are respectively 16.7 percent, 0.25 percent and 4.22mg/100 g. The content of soluble solids of the used abscisic acid, the content of titratable acid and the content of Vc are respectively 16.2 percent, 0.27 percent and 4.19mg/100 g. The content of soluble solid in the clear water, the content of titratable acid and the content of Vc are respectively 11.9 percent, 0.42 percent and 4.77mg/100 g.
Meanwhile, the coloring index of the apple is also determined according to the following formula
Figure BDA0002492607040000071
Figure BDA0002492607040000072
200 apples were treated for each treatment, with treatment 1 having a color index of 79.19%, treatment 2 having a color index of 75.26%, and clear water control having a color index of 63.14%. The results show that the product can meet the requirements in aspects of apple color enhancement and quality improvement.
Examples 3,
According to the weight portion ratio, 7.5 portions of lactobacillus rhamnosus, 1.5 portions of mineral calcium, 0.25 portion of mineral magnesium, 0.1 portion of mineral boron, 0.5 portion of mineral potassium and 0.15 portion of mineral iron are mixed to obtain a mixture; adding the mixture into culture medium, fermenting at 34 deg.C and ventilation ratio of 1:0.5 for 43 hr under the condition of pH of 6.5-7.5. Wherein the mixture accounts for 1% of the culture medium.
The lactobacillus rhamnosus fermentation medium is as follows: the formula of 1L is 10g of peptone, 10g of beef extract, 5g of yeast powder, 20g of glucose, 2g of triammonium citrate, 0.058g of magnesium sulfate heptahydrate, 3.12g of sodium acetate, 1.63g of disodium hydrogen phosphate, 2.25g of potassium acetate, 801 mL of tween, 0.25g of manganese sulfate tetrahydrate and the balance of water.
The experiment is designed with 2 treatments and 1 control, and the purple perilla is sprayed once in each seedling stage (emergence of seedlings and before branching), full growth stage (branching and before flowering), flowering stage (7-8 months) and fruit stage (8-9 months). Treatment 1: the color enhancer obtained in example 3 was diluted 800 times with water and sprayed to the foliage. And (3) treatment 2: 0.5mg/L of abscisic acid; control, clear water. The experiment was designed for three replicates, each cell area being 20 square meters, for a total of 180 square meters.
The fertilizer spraying is based on a conventional foliar fertilizer spraying method, and other management is the same as conventional management.
Sampling is carried out according to the field test requirements of the fertilizer, and the soluble solid content and the freezing point temperature are respectively measured, and the result shows that the soluble solid content and the freezing point temperature of the product are respectively 11.7 percent and 0.21 ℃. The content of soluble solids of the S-abscisic acid and the freezing temperature are respectively 10.9 percent and 0.42 ℃. The clear water control soluble solid content and freezing temperature are respectively 8.9 percent and 0.77 ℃. The results show that the product can affect the soluble solid which is an influencing factor for enhancing the color of the purple perilla.
Examples 4,
According to the parts by weight, 7.1 parts of lactobacillus rhamnosus, 1.9 parts of calcium, 0.25 part of magnesium, 0.1 part of boron, 0.5 part of potassium and 0.15 part of iron are mixed to obtain a mixture; adding the mixture into culture medium, fermenting at 34 deg.C and pH of 6.5-7.5 under aeration ratio of 1:0.5 for 48 hr. Wherein the mixture accounts for 2.5% of the culture medium.
The formula of the culture medium is as follows: the formula of 1L is 10g of peptone, 10g of beef extract, 5g of yeast powder, 20g of glucose, 2g of triammonium citrate, 0.058g of magnesium sulfate heptahydrate, 3.12g of sodium acetate, 1.63g of disodium hydrogen phosphate, 2.25g of potassium acetate, 801 mL of tween, 0.25g of manganese sulfate tetrahydrate and the balance of water.
The experiment is designed with 2 treatments and 1 control, and the rose is sprayed once for leaf expansion, bud emergence and full flower. Treatment 1: the color enhancer obtained in example 4 was diluted 1000 times with water and sprayed to the leaf surface. And (3) treatment 2: 0.5mg/L of abscisic acid; control, clear water. The experiment was designed for three replicates, each cell area being 20 square meters, for a total of 180 square meters.
The fertilizer spraying is based on a conventional foliar fertilizer spraying method, and other management is the same as conventional management.
The soluble sugar content and Vc are respectively measured by sampling according to the field test requirements of the fertilizer, and the results show that the soluble sugar content and Vc of the product are respectively 1.3 percent and 1975mg/100 g. The content of soluble sugar in the S-directed antibiotics and Vc were 1.0% and 1833mg/100g, respectively. The clear water content of soluble sugar and Vc are 0.77% and 1540mg/100g respectively. The results show that the product can increase the content of soluble sugar and Vc which are influencing factors for rose hyperchromia.

Claims (7)

1. A plant toner is characterized in that lactobacillus rhamnosus and a plurality of mineral substances are mixed and added into a culture medium, and fermentation is carried out for a certain time under a certain condition to obtain the plant toner;
the plant toner comprises 7-9 parts of lactobacillus rhamnosus, 1-2 parts of calcium, 0.25 part of magnesium, 0.1 part of boron, 0.5 part of potassium and 0.15 part of iron by weight;
the formula of the culture medium is as follows: 1000mL contains 10-12g of peptone, 10-12g of beef extract, 5-8g of yeast powder, 20-23g of glucose, 2-3g of triammonium citrate, 0.058-0.06g of magnesium sulfate heptahydrate, 3.12-3.17g of sodium acetate, 1.63-1.71g of disodium hydrogen phosphate, 2.25-2.5g of potassium acetate, 801-90 mL of tween, 0.25-0.3g of manganese sulfate tetrahydrate and the balance of water.
2. The plant toner of claim 1, wherein the fermentation conditions are: fermenting at pH of 6.5-7.5 and temperature of 30-34 deg.C and ventilation ratio of 1:0.3-0.5 for 40-50 h.
3. The plant toner of claim 1, wherein the amount of the mixture of lactobacillus rhamnosus and the plurality of minerals inoculated is 1-3% by mass of the medium.
4. The plant toner according to any one of claims 1, 2 or 3, wherein the plant is strawberry, apple, blueberry, cherry, kiwi, banana, rose, cabbage, tomato, eggplant.
5. Use of a plant toner according to any of claims 1, 2 or 3 as a fruit colorant.
6. Use of a plant toner according to any of claims 1, 2 or 3 as a plant conditioning fertilizer.
7. The method for using the plant toner as claimed in any one of claims 1, 2 or 3, wherein the toner is diluted by 800-fold with water and sprayed on the surface of the plant at different periods of plant growth.
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Citations (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20080105532A (en) * 2007-05-31 2008-12-04 청화산영농조합법인 Seedbed soil for growing blueberry and the method for preparing thereof
CN101613225A (en) * 2009-07-22 2009-12-30 山东光大肥业科技有限公司 A kind of special apple organic-inorganic compound fertilizer that contains Calcium of intensifiens, iron nutrition and preparation method thereof
KR101019133B1 (en) * 2010-08-19 2011-03-07 정현석 Plant nutrient substance for accelerating sugar content and coloration, and method of producing the same
CN102491797A (en) * 2011-11-14 2012-06-13 河海大学 Method for preparing chelating magnesium
JP2014080398A (en) * 2012-10-18 2014-05-08 Yukijirushi Shubyo Kk Plant growth regulator
CN104692925A (en) * 2015-02-04 2015-06-10 泰安市西贝隆基生物科技有限公司 Agricultural microbial ecological agent for soil conditioning and plant calcium supplement and production method thereof
CN105087680A (en) * 2015-08-19 2015-11-25 沈阳科纳提克生物科技有限公司 Lactobacillus fermentation culture medium and process for producing lactic acid at high yield
CN106011041A (en) * 2016-08-10 2016-10-12 青岛科拓恒通乳酸菌产业化开发研究院有限公司 Composite lactobacillus preparation used for increasing tomato quality and preparation method thereof
CN106116797A (en) * 2016-06-17 2016-11-16 张铭强 A kind of micro-carbon Anthocyanin promoting fruit coloring and completely filled fruit ahead of time and its preparation method and application
CN106119143A (en) * 2016-03-16 2016-11-16 河北润雨肥料科技有限公司 A kind of disease resistance growth promotion microbial-bacterial fertilizer and preparation method thereof
CN106358944A (en) * 2016-08-31 2017-02-01 周翠华 Cultivation method for increasing blueberry anthocyanin content and fruit internal quality
CN107667797A (en) * 2017-09-21 2018-02-09 界首市靳莓果酒有限责任公司 A kind of implantation methods for improving strawberry sugar content
CN108707563A (en) * 2018-05-28 2018-10-26 宁波吉丰生物科技发展有限公司 The preparation method of composite fermentation microbial inoculum and Fish protein amino acid Water soluble fertilizer
CN109136150A (en) * 2018-09-21 2019-01-04 郭春灵 A kind of complex microorganism liquid bacterial agent and its application
CN109593010A (en) * 2019-01-07 2019-04-09 天津新平台生物科技有限公司 A kind of preparation method and applications of high compound disease-resistant organic foliar fertilizer
CN110563503A (en) * 2018-06-06 2019-12-13 中国科学院沈阳应用生态研究所 Special fertilizer for grapes and preparation method thereof
CN110637082A (en) * 2017-01-04 2019-12-31 诺维信生物农业公司 Bacillus isolate and uses thereof

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101951765B (en) * 2008-02-19 2013-06-19 雪印种苗株式会社 Plant growth regulator composition
DK2836587T3 (en) * 2012-04-09 2017-05-08 Chr Hansen As Bioprotection using Lactobacillus rhamnosus strains
JP6296690B2 (en) * 2013-03-27 2018-03-20 サンスター株式会社 Fruit crushed material with suppressed color change and method for producing the same
CN103766474B (en) * 2014-01-14 2015-07-08 浙江大学 Biological preservative for cherry and tomato fruits and use thereof
AU2016243181A1 (en) * 2015-03-31 2017-08-31 Xyleco, Inc. Processing of biomass materials
US10202313B2 (en) * 2017-04-07 2019-02-12 Natural Organic Technologies, Inc. Plant supplements and related methods
CN108046913A (en) * 2017-12-21 2018-05-18 山西农业大学 A kind of fruit increases sugar and coloured composition, its preparation method and its application

Patent Citations (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20080105532A (en) * 2007-05-31 2008-12-04 청화산영농조합법인 Seedbed soil for growing blueberry and the method for preparing thereof
CN101613225A (en) * 2009-07-22 2009-12-30 山东光大肥业科技有限公司 A kind of special apple organic-inorganic compound fertilizer that contains Calcium of intensifiens, iron nutrition and preparation method thereof
KR101019133B1 (en) * 2010-08-19 2011-03-07 정현석 Plant nutrient substance for accelerating sugar content and coloration, and method of producing the same
CN102491797A (en) * 2011-11-14 2012-06-13 河海大学 Method for preparing chelating magnesium
JP2014080398A (en) * 2012-10-18 2014-05-08 Yukijirushi Shubyo Kk Plant growth regulator
CN104692925A (en) * 2015-02-04 2015-06-10 泰安市西贝隆基生物科技有限公司 Agricultural microbial ecological agent for soil conditioning and plant calcium supplement and production method thereof
CN105087680A (en) * 2015-08-19 2015-11-25 沈阳科纳提克生物科技有限公司 Lactobacillus fermentation culture medium and process for producing lactic acid at high yield
CN106119143A (en) * 2016-03-16 2016-11-16 河北润雨肥料科技有限公司 A kind of disease resistance growth promotion microbial-bacterial fertilizer and preparation method thereof
CN106116797A (en) * 2016-06-17 2016-11-16 张铭强 A kind of micro-carbon Anthocyanin promoting fruit coloring and completely filled fruit ahead of time and its preparation method and application
CN106011041A (en) * 2016-08-10 2016-10-12 青岛科拓恒通乳酸菌产业化开发研究院有限公司 Composite lactobacillus preparation used for increasing tomato quality and preparation method thereof
CN106358944A (en) * 2016-08-31 2017-02-01 周翠华 Cultivation method for increasing blueberry anthocyanin content and fruit internal quality
CN110637082A (en) * 2017-01-04 2019-12-31 诺维信生物农业公司 Bacillus isolate and uses thereof
CN107667797A (en) * 2017-09-21 2018-02-09 界首市靳莓果酒有限责任公司 A kind of implantation methods for improving strawberry sugar content
CN108707563A (en) * 2018-05-28 2018-10-26 宁波吉丰生物科技发展有限公司 The preparation method of composite fermentation microbial inoculum and Fish protein amino acid Water soluble fertilizer
CN110563503A (en) * 2018-06-06 2019-12-13 中国科学院沈阳应用生态研究所 Special fertilizer for grapes and preparation method thereof
CN109136150A (en) * 2018-09-21 2019-01-04 郭春灵 A kind of complex microorganism liquid bacterial agent and its application
CN109593010A (en) * 2019-01-07 2019-04-09 天津新平台生物科技有限公司 A kind of preparation method and applications of high compound disease-resistant organic foliar fertilizer

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
Effect of fermentation and sterilization on anthorcyanins in blueberry;Nie,Qixing;《Journal of the science of food and agriculture》;20170331(第97期);第1459-1466页 *
乳酸菌在农产品种植及其质量安全中的研究进展;高鹏飞;《中国农业科技导报》;20141231;第16卷(第6期);第143-148页 *
微生物菌剂对彩椒农艺性状及产量、品质的影响;李凤喜等;《蔬菜》;20160430(第4期);第15-16页 *
生物有机肥对提高土壤肥力及作物品质等综合效果研究;江志阳;《腐殖酸》;20160229(第1期);第39-44页 *
辣椒花青素合成相关基因的表达与紫色性状遗传分析;李珍;《中国优秀硕士学位论文全文数据库》;20160515(第05期);第D048-42页 *

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