CN111537707B - Chemiluminescent immunoassay analyzer, analytical system and detection method - Google Patents
Chemiluminescent immunoassay analyzer, analytical system and detection method Download PDFInfo
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- CN111537707B CN111537707B CN202010468119.7A CN202010468119A CN111537707B CN 111537707 B CN111537707 B CN 111537707B CN 202010468119 A CN202010468119 A CN 202010468119A CN 111537707 B CN111537707 B CN 111537707B
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- 238000001514 detection method Methods 0.000 title claims abstract description 82
- 238000003018 immunoassay Methods 0.000 title claims abstract description 43
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 113
- 238000002347 injection Methods 0.000 claims abstract description 59
- 239000007924 injection Substances 0.000 claims abstract description 59
- 238000001917 fluorescence detection Methods 0.000 claims abstract description 29
- 238000000034 method Methods 0.000 claims description 15
- 241000894006 Bacteria Species 0.000 abstract description 5
- 238000004458 analytical method Methods 0.000 abstract description 3
- 238000003908 quality control method Methods 0.000 description 7
- 241000187479 Mycobacterium tuberculosis Species 0.000 description 6
- 239000000243 solution Substances 0.000 description 5
- 238000011534 incubation Methods 0.000 description 4
- 238000003745 diagnosis Methods 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 206010059866 Drug resistance Diseases 0.000 description 2
- 241000208818 Helianthus Species 0.000 description 2
- 235000003222 Helianthus annuus Nutrition 0.000 description 2
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- 125000002485 formyl group Chemical class [H]C(*)=O 0.000 description 2
- 208000008128 pulmonary tuberculosis Diseases 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
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- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
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- 230000000721 bacterilogical effect Effects 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000012295 chemical reaction liquid Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000014670 detection of bacterium Effects 0.000 description 1
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- 229940088597 hormone Drugs 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
- G01N2035/00099—Characterised by type of test elements
- G01N2035/00158—Elements containing microarrays, i.e. "biochip"
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00178—Special arrangements of analysers
- G01N2035/00237—Handling microquantities of analyte, e.g. microvalves, capillary networks
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- Health & Medical Sciences (AREA)
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
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- Pathology (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
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Abstract
The invention discloses a chemiluminescent immunoassay analyzer, an analysis system and a detection method. The chemiluminescent immunoassay analyzer comprises: a chip tray for loading a microfluidic chip; a reagent injection mechanism for injecting a reagent into a detection region of the microfluidic chip; the driving mechanism is used for enabling the microfluidic chip on the chip tray to move relative to the reagent injection mechanism, and is connected with the chip tray; the fluorescence detection device is used for detecting the fluorescence intensity of the sample in the microfluidic chip and is provided with a detection port; the chip tray is provided with a reagent injection position for the reagent injection mechanism to be inserted into the microfluidic chip to push the reagent and a detection position for the fluorescence detection device to collect fluorescence, and when the chip tray is at the detection position, a detection area of the microfluidic chip can be opposite to the detection port. The invention can detect bacteria, and has convenient operation and compact structure.
Description
Technical Field
The invention belongs to the field of medical instruments, and relates to a chemiluminescent immunoassay analyzer, an analysis system and a detection method.
Background
In recent years, the incidence of pulmonary tuberculosis is on the rise, and the pulmonary tuberculosis is more and more concerned. Conventional methods such as acid-fast bacteria smear microscopic examination, mycobacterium tuberculosis separation culture and drug sensitivity experiments are still main methods for bacteriological diagnosis of all countries in the world after the organism is infected by the traditional mycobacterium tuberculosis, but the method has long detection time limit, lacks quick and accurate capability, and generally cannot meet the requirements of tuberculosis diagnosis. There is a critical need in clinic for a novel and direct rapid detection platform suitable for conventional mycobacterium tuberculosis identification. On one hand, the sensitivity and accuracy of diagnosis are required to be improved, the detection time is shortened, and on the other hand, bacterial drug resistance genes are detected, so that the reasonable use of antibiotics is guided, the curative effect is improved, and the generation of drug resistance bacteria is reduced. The chemiluminescent immunoassay (chemiluminescence immunoassay, CLIA) combines a chemiluminescent assay technology with high sensitivity and a high-specificity immune reaction, and is used for detection and analysis of various antigens, hapten, antibody, hormone, enzyme, fatty acid, vitamin, medicine and the like, and is a better solution.
Disclosure of Invention
Aiming at the technical problems, the invention aims to provide a chemiluminescent immunoassay analyzer, an analytical system and a detection method, which can detect bacteria and are convenient to operate.
In order to achieve the above purpose, the invention adopts the following technical scheme:
a chemiluminescent immunoassay analyzer comprising:
a chip tray for loading a microfluidic chip;
a reagent injection mechanism for injecting a reagent into a detection region of the microfluidic chip;
a driving mechanism for moving the microfluidic chip on the chip tray relative to the reagent injection mechanism, the driving mechanism being connected to the chip tray; and
A fluorescence detection device for detecting the fluorescence intensity of a sample in the microfluidic chip, the fluorescence detection device having a detection port;
the chip tray is provided with a reagent injection position for the reagent injection mechanism to be inserted into the microfluidic chip so as to push the reagent and a detection position for the fluorescence detection device to collect fluorescence, and when the chip tray is at the detection position, a detection area of the microfluidic chip can be opposite to the detection port.
Further, the reagent is a reagent pre-stored in the microfluidic chip.
Further, the chip tray is provided with a mounting groove into which the microfluidic chip can be clamped.
Further, the chip tray is slidably disposed on the guide rail.
Further, the reagent injection mechanism includes one or more push rods that can be inserted into the microfluidic chip.
Preferably, the reagent injection mechanism comprises a movable first push rod and a fixedly arranged second push rod.
More preferably, the reagent injection mechanism further comprises a mounting plate, the first push rod is movably arranged on the mounting plate, and the second push rod is fixedly arranged on the mounting plate.
More preferably, the mounting plate is fixedly provided with a push rod seat, the first push rod is movably arranged on the mounting plate in a penetrating manner along the length direction of the first push rod, one end part of the first push rod is movably inserted into the push rod seat, and an elastic piece is arranged between one end part of the first push rod and the push rod seat.
Further preferably, the elastic member is a compression spring propped between the one end portion of the first push rod and the push rod seat.
Further preferably, the length of the first push rod which can be inserted into the microfluidic chip is greater than the length of the second push rod which can be inserted into the microfluidic chip.
Further, the chip tray has a first reagent injection location corresponding to the first pushrod and a second reagent injection location corresponding to the second pushrod, the second reagent injection location being located between the first reagent injection location and the detection location.
Further, the driving mechanism comprises a motor, and an output shaft of the motor is connected with the chip tray.
Still further, the chemiluminescent immunoassay analyzer further comprises a control board, wherein the control board is electrically connected with the linear motor.
Further, the fluorescence detection device comprises a photomultiplier tube.
Further, the fluorescence detection device further comprises a counting device electrically connected with the photomultiplier.
Further, the chemiluminescent immunoassay analyzer further comprises a shell, wherein the chip tray, the reagent injection mechanism, the driving mechanism and the fluorescence detection device are arranged in the shell, and a window for loading the microfluidic chip on the chip tray is formed in the shell.
The invention also adopts the following technical scheme:
the chemiluminescence immunoassay system comprises a microfluidic chip and the chemiluminescence immunoassay analyzer, wherein the microfluidic chip is provided with a detection area and a reagent storage area, a micro-channel is arranged between the detection area and the reagent storage area, a plunger is arranged in the reagent storage area, and when the chip tray is at a reagent injection position, the plunger is abutted against the reagent injection mechanism.
The invention further adopts the following technical scheme:
a detection method of the chemiluminescent immunoassay analyzer as described above, comprising the steps of:
A. loading a microfluidic chip on a chip tray;
B. the driving mechanism drives the chip tray, the chip tray carries the microfluidic chip to move, and in the moving process of the microfluidic chip, the reagent injection mechanism is inserted into the microfluidic chip and extrudes a reagent pre-stored in the microfluidic chip into a detection area of the reagent injection mechanism;
C. the driving mechanism continues to drive the chip tray to move, and the chip tray carries the microfluidic chip to move below the fluorescence detection device so that the detection area of the microfluidic chip is opposite to the detection port of the fluorescence detection device.
Preferably, the step B specifically includes:
b1, the chip tray carries the micro-fluidic chip to move backwards until a quality control port of the micro-fluidic chip is opposite to a detection port of the fluorescence detection device; in the process, a first push rod of the reagent injection mechanism is inserted into the microfluidic chip;
b2, the chip tray carries the micro-fluidic chip to continue to move, and in the moving process of the micro-fluidic chip, a first plunger in the micro-fluidic chip is abutted against a first push rod of the reagent injection mechanism to be blocked, so that the reagent in the first reagent storage area is extruded into the detection area;
b3, the chip tray carries the micro-fluidic chip to further move, and in the process that the micro-fluidic chip continues to move, a second push rod of the reagent injection mechanism is inserted into the micro-fluidic chip and is abutted against a second plunger in the micro-fluidic chip, and the second plunger is subjected to the resistance of the second push rod to push the reagent in the second reagent storage area into the detection area.
Compared with the prior art, the invention has the following advantages:
the chemiluminescent immunoassay analyzer can be matched with a microfluidic chip, and can be used for injecting a reagent into a detection area through a reagent injection mechanism in the moving process of the microfluidic chip carried by a chip tray, realizing fluorescence collection detection through a fluorescence detection device, realizing detection of bacteria (such as mycobacterium tuberculosis), and adding the reagent and aligning the fluorescence detection device only by controlling the movement of the chip tray.
Drawings
In order to more clearly illustrate the technical solutions of the present invention, the drawings that are needed in the description of the embodiments will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of a chemiluminescent immunoassay analyzer according to an embodiment of the present invention;
FIGS. 2 and 3 are schematic partial structures of the chemiluminescent immunoassay analyzer shown in FIG. 1, respectively;
fig. 4a to 4f illustrate a moving process of the microfluidic chip.
Wherein,,
1. a chip tray; 10. a mounting groove; 11. a front limiting block; 12. a rear limiting block;
2. a reagent injection mechanism; 21. a first push rod; 22. a second push rod; 23. a mounting plate; 24. a push rod seat; 25. an elastic member;
3. a driving mechanism; 31. a linear motor;
4. a fluorescence detection device; 41. a photomultiplier tube; 411. a detection port; 42. a counting device;
5. a housing; 51. a window; 52. a linear guide rail; 53. a control board; 54. a power supply module; 55. a switch;
6. a microfluidic chip; 61. a detection zone; 62. a quality control port; 63. a first plunger; 64. and a second plunger.
Detailed Description
Preferred embodiments of the present invention will be described in detail below with reference to the attached drawings so that the advantages and features of the present invention can be more easily understood by those skilled in the art. The description of these embodiments is provided to assist understanding of the present invention, but is not intended to limit the present invention.
As used in this specification and in the claims, the terms "comprises" and "comprising" merely indicate that the steps and elements are explicitly identified, and do not constitute an exclusive list, as other steps or elements may be included in a method or apparatus. The term "and/or" as used herein includes any combination of one or more of the associated listed items.
It should be noted that, unless otherwise specified, when a feature is referred to as being "fixed" or "connected" to another feature, it may be directly or indirectly fixed or connected to the other feature. Further, the descriptions of the upper, lower, left, right, etc. used in the present invention are merely with respect to the mutual positional relationship of the constituent elements of the present invention in the drawings.
The present embodiment provides a chemiluminescent immunoassay analyzer. Referring to fig. 1, the chemiluminescent immunoassay analyzer has a housing 5, and a window 51 is formed in the housing 5, wherein the window 51 is used for mounting and dismounting a microfluidic chip 6. As shown in fig. 2 and 3, the chemiluminescent immunoassay analyzer includes a chip tray 1, a reagent injection mechanism 2, a driving mechanism 3, and a fluorescence detection device 4, which are disposed in a housing 5. The window 51 on the housing 5 is arranged opposite to the chip tray 1, and the microfluidic chip 6 enters and exits through the window 51 on the housing 5. The chip tray 1 is used for loading microfluidic chips 6. The reagent injection mechanism 2 is used for injecting a reagent into the detection zone of the microfluidic chip 6. The driving mechanism 3 is used for enabling the microfluidic chip 6 on the chip tray 1 to move relative to the reagent injection mechanism 2, and the driving mechanism 3 is connected with the chip tray 1 to drive the chip tray 1 to move. The fluorescence detection device 4 is used for detecting the fluorescence intensity of the sample in the microfluidic chip 6, and the fluorescence detection device 4 is provided with a detection port for fluorescence to enter. The chip tray 1 has a reagent injection position (as shown in fig. 4c and 4 d) and a detection position (as shown in fig. 4 e), when the chip tray 1 is at the reagent injection position, the reagent injection mechanism 2 is inserted into the microfluidic chip 6, and as the chip tray 1 continues to move, the reagent injection mechanism 2 moves relative to the microfluidic chip 6, pushing the reagent to inject the reagent into the detection region of the microfluidic chip 6; when the chip tray 1 is at the detection position, the chip tray 1 is located below the fluorescence detection device 4, the detection area of the microfluidic chip 6 loaded on the chip tray 1 can be opposite to the detection port of the fluorescence detection device 4, and then fluorescence in the detection area can enter the fluorescence detection device 4 through the detection port.
The chemiluminescent immunoassay analyzer is matched with the microfluidic chip 6, and the chemiluminescent immunoassay system further comprises the chemiluminescent immunoassay analyzer and the microfluidic chip 6. As shown in fig. 4a, the microfluidic chip 6 has a detection area 61 and a reagent storage area, a micro flow channel is provided between the detection area 61 and the reagent storage area, a plunger is provided in the reagent storage area, when the plunger is pushed by an external force, the reagent in the reagent storage area is extruded by the plunger to enter the detection area 61 through the micro flow channel, so as to realize incubation, reaction and the like, and the detection area 61 is used for performing reaction and detecting after the reaction. Specifically, two reagent storage areas are provided in the microfluidic chip 6, a first movable plunger 63 is provided in the first reagent storage area, and a second movable plunger 64 is provided in the second reagent storage area. In one specific embodiment, the first reagent storage area is pre-stored with phage buffer solution and the second reagent storage area is pre-stored with a sunflower aldehyde solution, and the chemiluminescent immunoassay can detect bacteria (e.g., mycobacterium tuberculosis) in a biological sample. The first reagent storage area and the second reagent storage area are both located at the rear side of the microfluidic chip 6. The detection region 61 is located substantially in the middle of the microfluidic chip 6, and has a hole for fluorescence light transmission. The microfluidic chip 6 is also provided with a quality control port 62, the quality control port 62 is positioned behind the detection area 61, the quality control port 62 can be used for pre-placing phage solution, and reaction liquid is added into the quality control port 62 when the microfluidic chip is used. .
As shown in fig. 3, the chip tray 1 is slidably disposed on the guide rail. The guide rail mainly comprises a pair of parallel linear guide rails 52, the linear guide rails 52 are fixedly arranged on the shell 5, and two sides of the chip tray 1 are respectively connected with the corresponding linear guide rails 52 in a sliding fit manner. The chip tray 1 is provided with a mounting groove 10 into which the microfluidic chip 6 can be clamped, and the shape of the mounting groove 10 in a plan view is consistent with that of the microfluidic chip 6. The front side of the chip tray 1 is provided with a front limiting block 11, the rear side of the chip tray 1 is provided with a rear limiting block 12, and the height of the front limiting block 11 is smaller than that of the rear limiting block 12, so that the microfluidic chip 6 can enter and exit the mounting groove 10. The number of the front limiting blocks 11 is two and the front limiting blocks are arranged at intervals, so that a person can clamp the microfluidic chip 6 conveniently and assemble and disassemble the microfluidic chip; the number of rear stoppers 12 is two and spaced apart to provide clearance for allowing the reagent injection mechanism 2 to move in and out of the microfluidic chip 6 to push the plunger. Here, the longitudinal direction of the linear guide 52 is defined as the front-rear direction, the side of the chip tray 1 that is farther from the reagent injection mechanism 2 is defined as the front side, and the side of the chip tray 1 that is closer to the reagent injection mechanism 2 is defined as the rear side.
As shown in connection with fig. 2 and 3, the reagent injection mechanism 2 comprises one or more push rods that can be inserted into the microfluidic chip 6. Further, after the push rod is inserted into the microfluidic chip 6, the push rod is matched with the plunger in the microfluidic chip 6, and as the chip tray 1 drives the microfluidic chip 6 to move, the plunger is blocked by the push rod to squeeze the reagent in front of the plunger into the detection area 61 of the microfluidic chip 6. The number of push rods is consistent with the number of plungers. Thus, the reagent injection mechanism 2 includes the first push rod 21 corresponding to the first plunger 63 and the second push rod 22 corresponding to the second plunger 64. As the microfluidic chip 6 moves backward, the first plunger 63 abuts against the first push rod 21 to receive a resistance of the first push rod 21, and when the resistance is greater than a friction force received by the first plunger 63 in the microfluidic chip 6, the first plunger 63 moves forward relative to the microfluidic chip 6 to push the reagent in the first reagent storage region into the detection region 61; similarly, the second plunger 64 is capable of pushing the reagent in the second reagent storage area into the detection zone 61.
Further, the first plunger 21 and the second plunger 22 do not move synchronously, and the second plunger 22 has not contacted the second plunger 64 when the first plunger 21 pushes the first plunger 63; after the reagent in the first reagent storage area is pushed into the detection area 61, a reaction time (e.g., incubation for 15min after phage buffer is pushed in) is required, and then the second push rod 22 pushes the reagent in the second reagent storage area into the detection area 61, at this time, the first push rod 21 may move backward together with the microfluidic chip 6 so that the second push rod 22 can contact the second plunger 64. Specifically, the first push rod 21 and the second push rod 22 are both disposed on a mounting plate 23, and the mounting plate 23 is fixedly disposed on the housing 5. Wherein the first push rod 21 is movably arranged on the mounting plate 23, and the second push rod 22 is fixedly arranged on the mounting plate 23. The mounting plate 23 is fixedly provided with a push rod seat 24, the first push rod 21 and the second push rod 22 are parallel to each other and extend along the front-rear direction, the first push rod 21 is movably arranged on the mounting plate 23 along the length direction (i.e. the front-rear direction), one end part of the first push rod 21 is movably inserted into the push rod seat 24, and an elastic piece 25 is arranged between one end part (i.e. the rear end part) of the first push rod 21 and the push rod seat 24. The elastic member 25 is specifically a compression spring that abuts against one end portion of the first push rod 21 and the push rod seat 24. The length of the first push rod 21, which can be inserted into the microfluidic chip 6, is longer than the length of the second push rod 22, which can be inserted into the microfluidic chip 6, so that the first push rod 21 contacts the first plunger 63 first, and after the reagent in the first reagent storage area is pushed in, the second push rod 22 contacts the second plunger 64, and the reagent pre-stored in the second reagent storage area is injected. Accordingly, the chip tray 1 has a first reagent injection position (shown in fig. 4 c) corresponding to the first push rod 21 and a second reagent injection position (shown in fig. 4 d) corresponding to the second push rod 22, the second reagent injection position being located between the first reagent injection position and the detection position (shown in fig. 4 e).
As shown in fig. 2 and 3, the driving mechanism 3 includes a motor, and an output shaft of the motor is connected to the chip tray 1. Specifically, the motor employs a linear motor 31, the linear motor 31 is mounted on the housing 5, and an output shaft of the linear motor 31 is connected to the chip tray 1 through a connector. As the linear motor 31 operates, the chip tray 1 moves in the front-rear direction accordingly.
As shown in fig. 2, the fluorescence detection device 4 includes a photomultiplier tube 41 and a counting device 42. The photomultiplier 41 (PMT) converts the fluorescence signal emitted from the detection port into an electrical signal, and the counting device 42 is electrically connected to the photomultiplier 41 to obtain the number of objects to be detected (such as mycobacterium tuberculosis) from the electrical signal output from the photomultiplier 41. The detection port specifically refers to a detection port of the photomultiplier tube 41, and is used for fluorescence to be provided inside the photomultiplier tube 41. In the present embodiment, the photomultiplier 41 is disposed above the chip tray 1, and the counting device 42 is disposed beside the chip tray 1.
As shown in fig. 2 and 3, the chemiluminescent immunoassay analyzer further comprises a control board 53, a power supply module 54, and a switch 55 for controlling the power supply module 54. The control board 53 is electrically connected to the linear motor 31, so as to send a control signal for controlling the start and stop of the linear motor 31 according to a set detection program. The control board 53 is also electrically connected to the counting device 42, so as to obtain the detection information obtained by the counting device 42, and display and output the detection information. The power supply module 54 is used for supplying power to the linear motor 31, the photomultiplier tube 41, the counting unit, the control board 53, and the like, and the power supply module 54 may be a power line for connecting with an external power source such as a mains supply, or may be a battery (e.g., a rechargeable battery).
The detection method of the chemiluminescent immunoassay analyzer comprises the following steps:
1. as shown in fig. 4a, the chip tray 1 is located at the front side of the guide rail, and even part of the chip tray can be located outside the housing 5 through the window 51, so that the microfluidic chip 6 is loaded on the chip tray 1;
2. the linear motor 31 operates, and the chip tray 1 carries the microfluidic chip 6 to move backwards along the linear guide rail 52 until the quality control port 62 of the microfluidic chip 6 is opposite to the detection port 411 of the photomultiplier 41, as shown in fig. 4 b; and in this process, the front portion of the first push rod 21 is inserted into the microfluidic chip 6, but the first plunger 63 has not yet been pushed;
3. the linear motor 31 continues to operate, the chip tray 1 carries the microfluidic chip 6 to move backwards along the linear guide rail 52, and in the moving process of the microfluidic chip 6, the first plunger 63 abuts against the first push rod 21 to be blocked, so that the reagent (such as phage buffer solution) in the first reagent storage area is extruded into the detection area 61, as shown in fig. 4 c; the linear motor 31 stops running, and the chip tray 1 maintains the first reagent injection position shown in fig. 4c for a period of time for reaction, incubation, etc., for example, 15min after squeezing into phage buffer;
4. after incubation, the linear motor 31 continues to run, the chip tray 1 carries the microfluidic chip 6 to further move backwards along the linear guide rail 52, and during the continuous moving process of the microfluidic chip 6, the second push rod 22 is inserted into the microfluidic chip 6 and abuts against the second plunger 64, and the second plunger 64 receives the resistance of the second push rod 22 to push the reagent (such as the sunflower aldehyde solution) in the second reagent storage area into the detection area 61, as shown in a second reagent injection position in fig. 4 d; in this process, the pushing force of the first plunger 63 applied to the first push rod 21 increases and moves rearward together with the first push rod 21, and the elastic member 25 is deformed by pressing;
5. the linear motor 31 runs reversely, and the chip tray 1 carries the microfluidic chip 6 to move forwards until the detection area 61 of the microfluidic chip 6 is opposite to the detection port 411 of the photomultiplier 41, as shown in fig. 4 e;
6. after the detection is completed, the linear motor 31 continues to move reversely, the chip tray 1 carries the microfluidic chip 6 to exit the window 51, and the microfluidic chip 6 is detached from the chip tray 1.
During the reverse running of the linear motor 31, the pushing force applied by the first plunger 63 to the first push rod 21 decreases until the pushing force disappears, and the restoring force of the elastic member 25 drives the first push rod 21 to restore for the next detection.
The above-described embodiments are provided for illustrating the technical concept and features of the present invention, and are intended to be preferred embodiments for those skilled in the art to understand the present invention and implement the same according to the present invention, not to limit the scope of the present invention. All equivalent changes or modifications made according to the principles of the present invention should be construed to be included within the scope of the present invention.
Claims (14)
1. A chemiluminescent immunoassay analyzer comprising:
a chip tray for loading a microfluidic chip;
a reagent injection mechanism for injecting a reagent into a detection region of the microfluidic chip;
a driving mechanism for moving the microfluidic chip on the chip tray relative to the reagent injection mechanism, the driving mechanism being connected to the chip tray; and
A fluorescence detection device for detecting the fluorescence intensity of a sample in the microfluidic chip, the fluorescence detection device having a detection port;
the reagent injection mechanism comprises one or more push rods which can be inserted into the microfluidic chip, the chip tray is provided with a reagent injection position for the reagent injection mechanism to be inserted into the microfluidic chip to push the reagent and a detection position for the fluorescence detection device to collect fluorescence, and when the chip tray is at the detection position, a detection area of the microfluidic chip can be opposite to the detection port.
2. The chemiluminescent immunoassay of claim 1 wherein: the chip tray is slidably arranged on the guide rail.
3. The chemiluminescent immunoassay of claim 1 wherein: the reagent injection mechanism comprises a movable first push rod and a second push rod which is fixedly arranged.
4. A chemiluminescent immunoassay according to claim 3 wherein: the reagent injection mechanism further comprises a mounting plate, the first push rod is movably arranged on the mounting plate, and the second push rod is fixedly arranged on the mounting plate.
5. The chemiluminescent immunoassay of claim 4 wherein: the mounting plate is fixedly provided with a push rod seat, the first push rod can be movably penetrated on the mounting plate along the length direction, and one end part of the first push rod is movably inserted into the push rod seat, and an elastic piece is arranged between the one end part of the first push rod and the push rod seat.
6. The chemiluminescent immunoassay of claim 5 wherein: the elastic piece is a pressure spring propped between one end part of the first push rod and the push rod seat.
7. The chemiluminescent immunoassay of claim 4 wherein: the length of the first push rod which can be inserted into the micro-fluidic chip is larger than the length of the second push rod which can be inserted into the micro-fluidic chip.
8. The chemiluminescent immunoassay analyzer of any one of claims 4 to 7 wherein: the chip tray has a first reagent injection location corresponding to the first pushrod and a second reagent injection location corresponding to the second pushrod, the second reagent injection location being located between the first reagent injection location and the detection location.
9. The chemiluminescent immunoassay of claim 1 wherein: the driving mechanism comprises a motor, and an output shaft of the motor is connected with the chip tray.
10. The chemiluminescent immunoassay of claim 1 wherein: the fluorescence detection device comprises a photomultiplier tube.
11. The chemiluminescent immunoassay of claim 10 wherein: the fluorescence detection device further comprises a counting device electrically connected with the photomultiplier.
12. The chemiluminescent immunoassay of claim 1 wherein: the chemiluminescent immunoassay analyzer further comprises a shell, wherein the chip tray, the reagent injection mechanism, the driving mechanism and the fluorescence detection device are arranged in the shell, and a window for loading a microfluidic chip on the chip tray is formed in the shell.
13. A chemiluminescent immunoassay system characterized by: the chemiluminescence immunoassay analyzer comprises a microfluidic chip and the chemiluminescence immunoassay analyzer according to any one of claims 1-12, wherein the microfluidic chip is provided with a detection area and a reagent storage area, a micro-channel is arranged between the detection area and the reagent storage area, a plunger is arranged in the reagent storage area, and when the chip tray is at a reagent injection position, the plunger is abutted against the reagent injection mechanism.
14. A method of detection of a chemiluminescent immunoassay according to any one of claims 1-12 comprising the steps of:
A. loading a microfluidic chip on a chip tray;
B. the driving mechanism drives the chip tray, the chip tray carries the microfluidic chip to move, and in the moving process of the microfluidic chip, the reagent injection mechanism is inserted into the microfluidic chip and extrudes a reagent pre-stored in the microfluidic chip into a detection area of the reagent injection mechanism;
C. the driving mechanism continues to drive the chip tray to move, and the chip tray carries the microfluidic chip to move below the fluorescence detection device so that the detection area of the microfluidic chip is opposite to the detection port of the fluorescence detection device.
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| CN112433063B (en) * | 2020-11-09 | 2021-07-09 | 昭衍(北京)医药科技有限公司 | Full-automatic chemiluminescence immunoassay technical platform |
| CN115244169B (en) * | 2021-01-29 | 2024-09-17 | 京东方科技集团股份有限公司 | Device and driving method for driving microfluidic chip |
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