CN111394220A - Nucleic acid extraction device - Google Patents
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- CN111394220A CN111394220A CN202010212449.XA CN202010212449A CN111394220A CN 111394220 A CN111394220 A CN 111394220A CN 202010212449 A CN202010212449 A CN 202010212449A CN 111394220 A CN111394220 A CN 111394220A
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Abstract
The invention discloses a nucleic acid extraction device. Nucleic acid extraction element includes base, centrifugal module and mixing module, and centrifugal module includes centrifugal tray, and centrifugal tray rotationally sets up on the base around centrifugal axis, and mixing module, including the test tube mounting, the test tube mounting has the test tube accommodation hole that is used for holding the test tube, and the test tube mounting rotationally sets up in centrifugal tray around mixing axis, and mixing axis sets up for centrifugal axis slope. The test tube fixing piece of the nucleic acid extracting device rotates relative to the centrifugal tray, and the mixing axis is inclined relative to the centrifugal axis, so that the rotating speed of the test tube fixing piece has a vertical component, so that the reagent in the test tube is moved upside down to better mix the reagent.
Description
Technical Field
The invention relates to the field of bioengineering, in particular to a nucleic acid extraction device.
Background
Nucleic acids are the most basic substances of life, and are composed of biological macromolecular compounds, which are vital to the whole living system. Nucleic acids have extremely important applications, both in practical use and in scientific research. Many genetic diseases are closely related to nucleic acids. In general, the study of nucleic acids is of great interest.
As a basic method of biomolecular science, nucleic acid extraction is the starting point for downstream nucleic acid detection, research analysis and product development, and is also a key step in nucleic acid diagnosis. Nucleic acid extraction has high requirements on the quality and integrity of the isolated nucleic acid, and has a crucial influence on both research and diagnosis, so that a rapid, effective and accurate nucleic acid extraction technology is a prerequisite for subsequent research. The aqueous two-phase system is a two-phase or multi-phase system which is formed by mixing some polymers, polymers and salt or other combinations in water at a certain concentration and is not mutually soluble. The two-aqueous phase nucleic acid extraction method is a novel nucleic acid extraction technology, and realizes the extraction of nucleic acid by extracting target nucleic acid by utilizing the difference of distribution coefficients of solutes in two phases.
According to the operation requirement of extracting nucleic acid by a double-aqueous phase nucleic acid extraction system, various instruments such as a centrifuge, a heating plate, an oscillator and the like are often required, and complicated manual operation is accompanied. At present, most of nucleic acid extraction apparatuses adopt a traditional nucleic acid extraction method. The whole extraction reaction time is long, the efficiency is low, the operation is complicated, the cost is high, and the automation degree is low.
Disclosure of Invention
The invention aims to provide a nucleic acid extraction device for better mixing reagents.
The present invention provides a nucleic acid extraction device, including:
a base;
the centrifugal module comprises a centrifugal tray, and the centrifugal tray is rotatably arranged on the base around a centrifugal axis; and
the mixing module, including the test tube mounting, the test tube mounting has the test tube accommodation hole that is used for holding the test tube, and the test tube mounting rotationally sets up in centrifugal tray around mixing axis, and mixing axis sets up for the slope of centrifugal axis.
In some embodiments, the mixing axis is inclined at an angle in the range of 15 ° to 45 ° relative to the centrifugal axis.
In some embodiments, the axis of the tube receiving aperture intersects the mixing axis.
In some embodiments, the axis of the cuvette-receiving bore is perpendicular to the mixing axis.
In some embodiments, the tube holder is configured to rotate forward and backward periodically with respect to the centrifuge tray.
In some embodiments, the nucleic acid extraction device includes two mixing modules symmetrically disposed about the axis of centrifugation.
In some embodiments, the centrifugal tray includes a central tray body and inclined tray bodies disposed at both sides of the central tray body, and the two mixing modules are respectively disposed on the two inclined tray bodies.
In some embodiments, mix the module still including setting up in the casing in the test tube mounting outside, the casing includes the cell body and rotates the lid of connecting on the cell body, is provided with test tube fastening strip on the inner wall of lid, and when the lid rotated the position to closed cell body, test tube fastening strip and the test tube lid butt of test tube were in order to compress tightly the test tube lid.
In some embodiments, the test tube mounting has a plurality of test tube accommodation holes that the interval set up, and the test tube fastening strip has spacing recess, and spacing recess extends so that test tube lid card locates in spacing recess along the distribution direction of a plurality of test tube accommodation holes.
In some embodiments, an elastomeric material is mounted within the retaining groove.
In some embodiments, the test tube mounting includes the connection limit that is located the bottom both sides, and the mixing module still includes the cushion that sets up between the bottom surface of connecting limit and casing, connects the limit and passes through the cushion and be connected with the casing.
In some embodiments, the nucleic acid extraction device further comprises a heating module including a heating member that heats the test tube holder.
In some embodiments, the heating element is an electrothermal film, and the electrothermal film is attached to the end face of the test tube fixing member.
In some embodiments, the heating module further comprises a temperature sensor, and the test tube holder further comprises a detection hole disposed between two adjacent test tube accommodating holes, and the temperature sensor is disposed in the detection hole.
In some embodiments, the nucleic acid extraction device further comprises a heat dissipation module, wherein the heat dissipation module comprises a side heat dissipation fan disposed on a side of the test tube holder; and/or, the heat dissipation module further comprises a bottom surface heat dissipation fan arranged on the bottom surface of the test tube fixing piece.
In some embodiments, the hybrid module includes a housing with fan louvers disposed thereon.
In some embodiments, the nucleic acid extraction device further comprises a control module comprising a primary controller coupled to the centrifugation module, a secondary controller coupled to the mixing module, and a conductive slip ring connected to the centrifugation tray and electrically connecting the primary controller and the secondary controller.
Based on the technical scheme provided by the invention, the nucleic acid extraction device comprises a base, a centrifugal module and a mixing module, wherein the centrifugal module comprises a centrifugal tray which is rotatably arranged on the base around a centrifugal axis, the mixing module comprises a test tube fixing piece, the test tube fixing piece is provided with a test tube accommodating hole for accommodating a test tube, the test tube fixing piece is rotatably arranged on the centrifugal tray around a mixing axis, and the mixing axis is obliquely arranged relative to the centrifugal axis. The test tube fixing piece of the nucleic acid extracting device rotates relative to the centrifugal tray, and the mixing axis is inclined relative to the centrifugal axis, so that the rotating speed of the test tube fixing piece has a vertical component, so that the reagent in the test tube is moved upside down to better mix the reagent.
Other features of the present invention and advantages thereof will become apparent from the following detailed description of exemplary embodiments thereof, which proceeds with reference to the accompanying drawings.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the invention without limiting the invention. In the drawings:
FIG. 1 is a schematic view of the structure of a nucleic acid isolation apparatus according to an embodiment of the present invention;
FIG. 2 is a schematic structural diagram of the centrifuge module of FIG. 1;
FIG. 3 is a schematic diagram of the mixing module and centrifuge tray of FIG. 1;
FIG. 4 is a schematic view of the connection structure of the mixing module and the centrifuge tray in FIG. 3;
FIG. 5 is a schematic view of the mixing module and heating module within the housing of FIG. 3;
FIG. 6 is a schematic view of the heating module of FIG. 5 with the heat dissipation fan removed;
FIG. 7 is a schematic diagram of the control module of FIG. 1;
FIG. 8 is a schematic diagram of the control principle of the control module shown in FIG. 7.
Each reference numeral represents:
1. a base;
2. a centrifuge module;
21. a centrifugal tray; 211. a central disc body; 212. inclining the tray body; 213. a vertical tray body; 22. a direct current motor; 221. an output shaft of the DC motor; 23. a coupling; 25. an extension shaft; 26. a shaft sleeve; 27. a motor tray; 28. a motor bracket;
3. a mixing module;
31. a housing; 311. a trough body; 311A, a fan heat dissipation hole; 311B, ventilating and radiating holes; 312. a cover body; 32. a test tube holder; 321. a cuvette-receiving hole; 322. a connecting edge; 33. cushion blocks; 34. locking; 35. a stepping motor; 36. bearing fixing seats, 37 and bearings; 38. a step motor shaft sleeve; 39. a test tube fastening strip;
4. a control module;
41. a main controller; 42. a secondary controller; 43. a conductive slip ring;
5. a heating module;
6. a heat dissipation module;
61. a side heat radiation fan; 62. a bottom heat radiation fan.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. The following description of at least one exemplary embodiment is merely illustrative in nature and is in no way intended to limit the invention, its application, or uses. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The relative arrangement of the components and steps, the numerical expressions and numerical values set forth in these embodiments do not limit the scope of the present invention unless specifically stated otherwise. Meanwhile, it should be understood that the sizes of the respective portions shown in the drawings are not drawn in an actual proportional relationship for the convenience of description. Techniques, methods, and apparatus known to those of ordinary skill in the relevant art may not be discussed in detail, but are intended to be part of the specification where appropriate. In all examples shown and discussed herein, any particular value should be construed as merely illustrative, and not limiting. Thus, other examples of the exemplary embodiments may have different values. It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, further discussion thereof is not required in subsequent figures.
Spatially relative terms, such as "above … …," "above … …," "above … …," "above," and the like, may be used herein for ease of description to describe one device or feature's spatial relationship to another device or feature as illustrated in the figures. It will be understood that the spatially relative terms are intended to encompass different orientations of the device in use or operation in addition to the orientation depicted in the figures. For example, if a device in the figures is turned over, devices described as "above" or "on" other devices or configurations would then be oriented "below" or "under" the other devices or configurations. Thus, the exemplary term "above … …" can include both an orientation of "above … …" and "below … …". The device may be otherwise variously positioned and the spatially relative descriptors used herein interpreted accordingly.
As shown in fig. 1 to 6, the nucleic acid extraction apparatus according to the embodiment of the present invention includes:
a base 1;
the centrifugal module 2 comprises a centrifugal tray 21, and the centrifugal tray 21 is rotatably arranged on the base 1 around a centrifugal axis; and
mixing module 3, including test tube holder 32, test tube holder 32 has a test tube accommodation hole 321 for accommodating test tube a, and test tube holder 32 rotationally sets up in centrifugal tray 21 around the mixing axis, and the mixing axis sets up for the centrifugation axis slope.
The tube holder 32 of the nucleic acid isolation apparatus according to the embodiment of the present invention rotates with respect to the centrifugal tray 21 and the mixing axis is inclined with respect to the centrifugal axis, so that the rotation speed of the tube holder 32 has a vertical component to allow the reagent in the tube a to move upside down for better mixing the reagent.
In order to provide a better centrifugation effect for the reagents, the inclination angle of the mixing axis with respect to the centrifugation axis in this example is in the range of 15 ° to 45 °.
As shown in fig. 3, the mixing module 3 of the present embodiment further includes a housing 31 disposed outside the test tube holder 32 and fixedly connected to the test tube holder 32. The housing 31 has a square structure and includes a slot 311 and a cover 312 rotatably connected to the slot 311. The cover 312 is connected to the slot 311 by the lock catch 34 to lock the housing 31. Specifically, the lock catch 34 includes a box buckle disposed on the slot 311 and a hook disposed on the cover 312, and the hook is engaged with the box buckle.
In the following description, the cover 312 side is referred to as an upper side, and the side opposite to the cover 312 is referred to as a lower side. As shown in fig. 6, the test tube holder 32 of the present embodiment includes connecting edges 322 located at two sides of the bottom, the mixing module 3 further includes a pad block 33 disposed between the connecting edges 322 and the bottom surface of the housing 31, and the connecting edges 322 are connected to the housing 31 through the pad block 33.
The mixing module 3 of the present embodiment further includes a stepping motor 35 for driving the housing 31 to rotate. The output shaft of the stepping motor 35 is connected to a stepping motor boss 38, and the stepping motor boss 38 is connected to the side wall of the housing 31. The step motor shaft sleeve 38 is installed in the inner diameter of the bearing 37 and forms a tight fit with the inner ring structure of the bearing 37, the outer ring of the bearing 37 is installed in the bearing fixing seat 36, and the bearing fixing seat 36 is fixed on the centrifugal tray 21 through screws. The output shaft of the stepping motor drives the mixing module 3 to rotate, and the effect of mixing the reagent is achieved. And after the hybrid module 3 is fixed, the weight of the hybrid module is concentrated on the bearing fixing seat 36, and the stepping motor 35 only plays a role of transmission and cannot bear the weight of the whole hybrid module 3.
In order to better mix the reagents, the tube holder 32 of the present embodiment is configured to rotate forward and backward periodically with respect to the centrifuge tray 21. Specifically, the output shaft of the stepping motor 35 is controlled to periodically rotate forward and backward, so as to drive the housing 3 and the test tube fixing member 32 to periodically rotate forward and backward. As shown in fig. 6, the cuvette holder 32 according to the present embodiment has a plurality of cuvette receiving holes 321 arranged at intervals, and the plurality of cuvette receiving holes 321 are arranged in order along a straight line, thereby achieving an operation of extracting nucleic acid from a plurality of reagents to improve efficiency.
To further mix the reagents uniformly, the axis of the cuvette receiving hole 321 of the present embodiment intersects the mixing axis. So set up, thereby reagent in test tube A's the axis direction motion makes the abundant mixing of reagent. The tube holding member 32 of the present embodiment includes a plurality of tube receiving holes 321, wherein the axis of the tube receiving holes may be in the same plane as the mixing axis and intersect with each other, and the axis of the tube receiving holes may not be in the same plane as the mixing axis but intersect with each other.
Specifically, the axis of the tube accommodating hole 321 is perpendicular to the mixing axis. When the axis of test tube accommodation hole 321 is perpendicular with the mixing axis, test tube a follows test tube mounting 32 and rotates around the mixing axis, and test tube a's linear velocity direction is the axis of test tube accommodation hole 321, and the reagent speed that is located in test tube a is the same direction so, and in the process that test tube mounting 32 is followed casing 31 and is done periodic positive and negative rotation, the reagent in test tube a will do the reversal along test tube a's axis direction. The arrangement ensures that the reagent has sufficient space motion in the process of up-down reversal mixing, and simultaneously ensures that the reagent is centrifuged at the angle, so that the reagent can obtain better centrifugation effect.
The nucleic acid extraction apparatus of the present embodiment further includes a heating module 5. The heating module 5 includes a heating member that heats the test tube holder 32.
Specifically, the test tube holder 32 of the present embodiment is a metal block. The heating member is an electrothermal film which is attached to the end face of the test tube fixing member 32. Preferably, the end faces of the two sides of the test tube fixing member 32 of this embodiment are both attached with an electrothermal film so that the test tube accommodating hole 321 is located between the two electrothermal films to ensure the stability of heating. And the electrothermal film covers the surface of the metal block in a large area to improve the heating efficiency.
In other embodiments, the heating element may also be an electric heating tube, an electric heating ceramic sheet, or other heating elements, and is not limited to heating by using electric energy.
The terminal surface of the test tube fixing part 32 of this embodiment is provided with the mounting groove, and the electric heat membrane is embedded into the mounting groove in order to reach good heating effect.
The diameter of the test tube accommodation hole 321 of this embodiment is slightly greater than the diameter of the test tube so that the test tube can be better attached to the wall of the test tube accommodation hole to ensure the direct conduction of heat when the test tube is inserted into the test tube accommodation hole 321. Meanwhile, the wall thickness between the adjacent test tube accommodating holes is the same so as to ensure the uniformity and stability of heat conduction in the heating process and reduce the temperature difference between the holes.
In order to monitor the heating temperature, the heating module 5 of the present embodiment further includes a temperature sensor, and the test tube fixing member 32 further includes a detection hole disposed between two adjacent test tube accommodating holes, and the temperature sensor is disposed in the detection hole. For example, the inspection hole is opened in the middle of first test tube accommodation hole and second test tube accommodation hole, can better reflect the temperature of first test tube accommodation hole and second test tube accommodation hole. Because the electric heat membrane is the symmetry setting and the test tube accommodation hole also sets up the centre at the test tube mounting, consequently the heating process also is the symmetry and goes on, and temperature transmission is also symmetrical, so the temperature between first test tube accommodation hole and the second test tube accommodation hole also can reflect the temperature between third test tube accommodation hole and the fourth test tube accommodation hole.
For avoiding the inside temperature rise pressure of test tube A increase and make the test tube lid open and lead to the problem of reagent leakage in the heating process, the mixing module 3 of this embodiment is still including setting up test tube fastening strip 39 on the inner wall of lid 312, when lid 312 rotates to the position of closed cell body 311, thereby test tube fastening strip 39 prevents that the test tube lid from breaking away with the test tube lid butt of test tube A in order to compress tightly the test tube lid.
Specifically, the test tube fastening strip 39 of the present embodiment has a limit groove extending in the distribution direction of the plurality of test tube accommodating holes 321 so that the plurality of test tube caps are caught in the limit groove.
The limiting groove of the embodiment is internally provided with an elastic material. When closing lid 312, elastic material and test tube cover contact, make elastic material and test tube cover contact and produce certain deformation in order to last exert pressure to the test tube cover and play the logical of fastening test tube through control elastic material's thickness.
After the reagent is mixed, the reagent in the test tube needs to be cooled. In this embodiment, the nucleic acid extraction apparatus further comprises a heat dissipation module 6, wherein the heat dissipation module 6 comprises a side heat dissipation fan 61 disposed on a side surface of the heating module 5; and/or, the heat dissipation module 6 comprises a bottom surface heat dissipation fan 62 arranged on the bottom surface of the heating module 5.
As shown in fig. 3, the housing 31 of the present embodiment is provided with a fan heat dissipation hole 311A on a surface corresponding to the heat dissipation fan, and specifically, the housing 31 is provided with a fan heat dissipation hole 311A on both side surfaces and a bottom surface.
The slot 311 of this embodiment is a square structure, and includes four side surfaces and a bottom surface that are circumferentially arranged, wherein one side surface is a connection side surface connected with the centrifugal tray 21, and two opposite side surfaces and the bottom surface are provided with fan heat dissipation holes 311A. As shown in fig. 3, the lower side of the fan heat dissipation hole 311A is further provided with a strip-shaped hole, and the strip-shaped hole is used for penetrating a power supply circuit of the heat dissipation fan and the like so as to facilitate the arrangement and layout of the circuit. And the strip-shaped holes can further improve the heat dissipation effect.
In order to further improve the heat dissipation effect, the side surface of the slot body 311 opposite to the connecting side surface of the present embodiment is further provided with a plurality of ventilation and heat dissipation holes 311B.
In order to maintain the balance of centrifugation, as shown in FIG. 1, the nucleic acid extraction apparatus of the present embodiment includes two mixing modules 3 disposed symmetrically with respect to the axis of centrifugation.
As shown in fig. 2, the centrifugal tray 21 includes a central tray body 211 and inclined tray bodies 212 disposed at both sides of the central tray body 211, and the two mixing modules 3 are respectively disposed on the two inclined tray bodies 212.
The centrifuge module 2 of the present embodiment further includes a centrifuge driving means for driving the centrifuge tray 21 to rotate. Specifically, the centrifugal driving device of the present embodiment is a dc motor 22, and an output shaft 221 of the dc motor 22 is connected to the extension shaft 25 through a coupling 23. The end of the extension shaft 25 is sleeved with a shaft sleeve 26, and the shaft sleeve 26 is connected with the centrifugal tray 21.
In the present embodiment, as shown in FIGS. 7 and 8, the nucleic acid extracting apparatus further includes a control module 4, and the control module 4 includes a main controller 41 coupled to the centrifugal module 2, a sub-controller 42 coupled to the mixing module 3, and a conductive slip ring 43, and the conductive slip ring 43 is connected to the centrifugal tray 21 and electrically connects the main controller 41 and the sub-controller 42.
Wherein, the main controller 41 is disposed on the base 1.
Since the mixing module 3 and the heating module 5 are both rotated along with the centrifugal tray 21 and the sub-controller 42 is for controlling the mixing module 3 and the heating module 5, in order to reduce the connecting wires between the control module and the mixing module 3 and the heating module 5 to simplify the apparatus, the sub-controller 42 of the present embodiment is rotated in synchronization with the centrifugal tray 21.
Specifically, the centrifugal tray 21 of the present embodiment further includes a vertical tray 213 connected to the inclined tray 212, and the sub-controller 42 is connected to the vertical tray 213 and rotates along with the vertical tray 213.
The structure and operation of the nucleic acid isolation apparatus according to the embodiment of the present invention will be described in detail with reference to FIGS. 1 to 8.
As shown in FIGS. 1 to 7, the nucleic acid extraction apparatus of the present embodiment includes a base 1, a centrifugation module 2, a mixing module 3, a control module 4, a heating module 5, and a heat dissipation module 6. Base 1 is fixed to be set up, and centrifugal module 2 rotationally sets up on base 1, and mixing module 3 rotationally sets up on centrifugal module 2.
The centrifugal module 2 comprises a centrifugal tray 21, a direct current motor 22, a coupling 23, an extension shaft 25, a shaft sleeve 26, a motor tray 27 and a motor support 28. Two motor supports 28 are fixed on base 1 side by side, and motor tray 27 is connected in the top of motor support 8, and direct current motor 22's top is connected with motor tray 27 and direct current motor 22's bottom and base 1 between have certain interval. The output shaft 221 of the dc motor 22 passes through the motor tray 27 and is connected to the extension shaft 25 through the coupling 23. The extension shaft 25 is inserted into a hole of the bushing 26 and connected with the bushing 26 by a screw connection. The centrifugal tray 21 is fixed to the upper surface of the sleeve 25. The direct current motor 22 has a wider rotation speed adjusting range, provides a larger torque to drive the centrifugal tray 21 to rotate at a low rotation speed, and ensures a good centrifugal effect at a high rotation speed.
The nucleic acid extraction device of the present embodiment includes two mixing modules 3 symmetrically disposed with respect to the centrifugal axis so that the centrifugal turntable 21 of the present embodiment is strictly symmetrical from side to side, ensuring the stability of centrifugation.
As shown in fig. 3 to 6, the mixing module 3 of the present embodiment includes a housing 31, a test tube holder 32, a spacer 33, a latch 34, a stepping motor 35, a bearing holder 36, a bearing 37, a stepping motor hub 38, and a test tube fastening strip 39.
As shown in fig. 3, the housing 31 includes a slot 311 and a cover 312. The test tube holder 32 is fixedly disposed in the housing 31. As shown in fig. 5, the test tube holder 32 is provided with a plurality of test tube accommodating holes 321 arranged in a line in sequence, and the test tubes a containing the reagent are placed in the test tube accommodating holes 321. And test tube mounting 32 includes connecting edge 322 that is located the bottom both sides, and connecting edge 322 is connected with the bottom surface of casing 31 and is provided with cushion 33 between connecting edge 322 and the bottom surface of casing 31 so that have a certain distance between test tube mounting 32 and the bottom surface of casing 31.
As shown in fig. 3, an end surface of the stepping motor 35 is fixed to the lower surface of the centrifugal tray 21 by screws. The output shaft of the stepping motor 35 is inserted into the circular hole of the stepping motor boss 38. The step motor shaft sleeve 38 is installed in the inner diameter of the bearing 37 and forms a tight fit with the inner ring structure of the bearing 37, the outer ring of the bearing 37 is installed in the bearing fixing seat 36, and the bearing fixing seat 36 is fixed on the centrifugal tray 21 through screws. The mixing module 3 is driven by a stepper motor 35 to effect a circular motion relative to the centrifuge tray 21 via bearings 37 to effect mixing of the reagents. And after the hybrid module 3 is fixed, the weight of the hybrid module is concentrated on the bearing fixing seat 36, and the stepping motor 35 only plays a role of transmission and cannot bear the weight of the whole hybrid module 3.
Specifically, the test tube fastening strip 39 of this embodiment is a metal strip, and has a rectangular groove formed thereon. The groove is internally provided with a rubber strip with certain elasticity. When the cap body 312 is closed, the test tube fastening strip 39 comes into contact with the test tube held in the test tube holder 32. Through the thickness of control rubber strip, make rubber strip and test tube lid contact at the actual test in-process, produce certain deformation, last exert pressure to the test tube lid, play the effect of fastening the test tube. Meanwhile, the risk that reagent leakage occurs when the test tube cover is opened by steam generated by high-temperature heated gas in the test tube is reduced.
The nucleic acid extraction device of this embodiment further includes a heating module 5, and the heating module 5 includes a heating member connected to the test tube holder 32, and the heating member heats the test tube a through the test tube holder 32.
Specifically, the heating member of this embodiment is the electric heat membrane, and the both sides of test tube mounting 32 are all pasted and are equipped with the electric heat membrane. The test tube mounting 32 of this embodiment is massive structure, and it has the electric heat membrane to be embedded respectively on its both ends face, is the tandem, covers the surface of test tube mounting 32 by a large scale to press from both sides test tube accommodation hole 321 in the middle of two electric heat membranes, guarantee the stability of heating.
Still be provided with the inspection hole on the test tube mounting 32 of this embodiment, placed temperature sensor in the inspection hole, and the inspection hole is inside to be filled with heat conduction silicone grease, guarantees that the temperature in the inspection hole can be better must follow the temperature of test tube mounting 32. The inspection hole is opened in the middle of two adjacent test tube accommodation holes, can better reflect the temperature of two test tube accommodation holes.
The heat dissipation module 6 of the present embodiment includes a side heat dissipation fan 61 disposed on the side surface of the test tube holder 32 and a bottom heat dissipation fan 62 disposed on the bottom surface of the test tube holder 32. The housing 31 of the present embodiment is provided with a fan heat radiation hole 311A at a position corresponding to the heat radiation fan. The side heat dissipation fan 61 blows air outward from the housing 31. The bottom heat dissipation fan 62 blows air into the housing 31. In the heat dissipation process, all the heat dissipation fans are turned on, the bottom heat dissipation fan 62 blows air into the casing 31, and the side heat dissipation fans 61 on both sides draw air out of the casing 31. The air at the outside room temperature is blown on the bottom surface of the test tube fixing member 32, and the heat of the test tube fixing member 32 is taken away by the wind and dissipated by the side heat dissipation fans 61 at the two sides, so that a good air heat dissipation effect is achieved. And because the corresponding side of casing 31 is opened has corresponding fan louvre 311A, even remove the radiator fan after, fan louvre 311A also can be as the ventilation hole, when carrying out centrifugal operation through centrifugal module 2 to mixing module 3, the outside air can enter into casing 31 through the fan louvre and play the heat dissipation effect. At this time, the rotation speed of the centrifugal module 2 can be controlled to realize the rapid flow of the air inside the shell 31, so as to achieve a good heat dissipation effect.
Besides the fan heat dissipation holes 311A, the housing 31 of the present embodiment is further provided with ventilation heat dissipation holes 311B to further improve the heat dissipation effect. The housing 31 of this embodiment is provided with a plurality of ventilation and heat dissipation holes 311B arranged side by side,
the nucleic acid extraction apparatus of the present embodiment further includes a control module 4. As shown in fig. 8, the control module 4 includes a master controller 41 and two slave controllers 42 corresponding to the two mixing modules 3. The master controller 41 and slave controller 42 employ conductive slip rings 43 to solve the electrical routing problem. As shown in fig. 2, the conductive slip ring 43 is disposed on the extension shaft 25. Specifically, the conductive slip ring 43 includes a stator end and a rotor end, the stator end is fixed on the motor support 28 through a fixing support, the stator end electrical signal line is connected with the main controller 41, the rotor end is fixed with the extension shaft 25, and the rotor end electrical signal line is connected with the slave controller 42, so that the main controller and the slave controller are electrically connected, and power current and signal transmission can be realized. The conductive slip ring is internally provided with a brush and a bearing structure, and the conductive slip ring keeps sliding contact with the brush when rotating so as to transmit power current and signals. The conductive slip ring structurally adopts high-temperature alloy and composite materials, and cooling is not needed in the using process.
The centrifugal module 2 is controlled by a main controller 41, the mixing module 3 and the heating module 5 are controlled by a secondary controller 42, and the two secondary controllers 42 are respectively fixed at two sides of the centrifugal tray 21 and respectively control the functional modules at the two sides. The main controller 41 is connected with a plurality of groups of buttons and indicator lamps, the buttons are used for issuing commands to the main controller 41, the main controller 41 judges the commands and then communicates with the slave controller 422, corresponding operations are executed, and the main controller 41 controls the indicator lamps to reflect the running state of the device. The user can use the button to operate the device and the indicator light can read the device operating state.
The operation of the nucleic acid isolation apparatus of this example was as follows:
the nucleic acid reagent is put into the test tube A, the test tube A is put into the test tube accommodating hole 321 in the test tube holder 32, and is locked by the test tube fastening strip 39. In the mixing process, the output shaft of the stepping motor 35 makes a periodic circular motion to drive the housing 31 to rotate, at this time, the test tube a placed in the housing 31 makes an up-down reversal motion, and the nucleic acid reagent in the test tube a is also reversed up and down to achieve the purpose of reversing and uniformly mixing the nucleic acid reagent in the test tube a. Through heating control to the electric heat membrane, make the inside reagent of test tube A obtain the heating. The heating control can be performed simultaneously with the mixing control, that is, when the mixing module 3 performs the mixing operation, the test tube a moves upside down, and the heating module 5 performs the heating control on the reagent simultaneously.
After mixing and heating are accomplished, need realize centrifugal separation to reagent, under the state of guaranteeing 3 left and right sides symmetries of mixed module, centrifugal module 2 carries out centrifugal operation, and the mixed module 3 and the heating module 5 of installing on centrifugal tray 21 are driven and are centrifuged together, make the interior reagent of test tube A realize the separation under the effect of centrifugal force.
The heating module 5 of the present embodiment is installed in the mixing module 3, and both are installed on the centrifuge module 2, so that the reagents can be reversely mixed along a certain angle on the slope while being accompanied with heating control, and can also make high-speed circular motion along the centrifuge axis. Meanwhile, the test tube A is placed at a certain angle, so that the reagent can be separated under the action of centrifugal force. The nucleic acid extraction device of the embodiment integrates three functional modules on one platform, selects combinations of different functions according to the requirements of the nucleic acid extraction process, and the modules cooperate to complete the automatic nucleic acid extraction process. Wherein, mixing module and heating module are protected by metal casing, prevent that maloperation from causing the injury to personnel when high temperature heating, high-speed centrifugation.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention and not to limit it; although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art will understand that: modifications to the specific embodiments of the invention or equivalent substitutions for parts of the technical features may be made; without departing from the spirit of the present invention, it is intended to cover all aspects of the invention as defined by the appended claims.
Claims (17)
1. A nucleic acid extraction device, comprising:
a base (1);
a centrifuge module (2) comprising a centrifuge tray (21), the centrifuge tray (21) being rotatably arranged on the base (1) about a centrifuge axis; and
mixing module (3), including test tube mounting (32), test tube mounting (32) have a test tube accommodation hole (321) that is used for holding test tube (A), just test tube mounting (32) around mixing axis rotationally set up in centrifuge tray (21), mixing axis for the centrifuge axis slope sets up.
2. The nucleic acid isolation apparatus according to claim 1, wherein the inclination angle of the mixing axis with respect to the centrifugal axis is in the range of 15 ° to 45 °.
3. The nucleic acid extraction device according to claim 1, wherein an axis of the cuvette-accommodating hole (321) intersects the mixing axis.
4. The nucleic acid extraction apparatus according to claim 3, wherein an axis of the cuvette accommodating hole (321) is perpendicular to the mixing axis.
5. The nucleic acid extraction apparatus according to claim 1, wherein the tube holder (32) is configured to rotate periodically in the forward and reverse directions with respect to the centrifuge tray (21).
6. The nucleic acid extraction apparatus according to claim 1, characterized in that it comprises two mixing modules (3) arranged symmetrically with respect to the axis of centrifugation.
7. The nucleic acid extraction apparatus according to claim 6, wherein the centrifugation tray (21) includes a central tray body (211) and inclined tray bodies (212) provided on both sides of the central tray body (211), and the two mixing modules (3) are respectively provided on the two inclined tray bodies (212) in correspondence.
8. The nucleic acid extraction device according to claim 1, wherein the mixing module (3) further comprises a housing (31) disposed outside the test tube holder (32), the housing (31) comprises a groove (311) and a cover (312) rotatably connected to the groove (311), a test tube fastening strip (39) is disposed on an inner wall of the cover (312), and when the cover (312) is rotated to close the position of the groove (311), the test tube fastening strip (39) abuts against a test tube cover of the test tube (A) to compress the test tube cover.
9. The nucleic acid extraction device according to claim 8, wherein the cuvette holder (32) has a plurality of cuvette-accommodating holes (321) arranged at intervals, and the cuvette fastening strip (39) has a stopper groove extending in a direction in which the plurality of cuvette-accommodating holes (321) are distributed so that the cuvette lid is caught in the stopper groove.
10. The nucleic acid extraction device of claim 9, wherein an elastic material is installed in the limiting groove.
11. The nucleic acid extraction apparatus according to claim 8, wherein the test tube holder (32) includes a connecting edge (322) on both sides of the bottom, and the mixing module (3) further includes a spacer (33) provided between the connecting edge (322) and the bottom surface of the housing (31), and the connecting edge (322) is connected to the housing (31) through the spacer (33).
12. The nucleic acid extraction device according to any one of claims 1 to 11, further comprising a heating module (5), the heating module (5) comprising a heating element that heats the test tube holder (32).
13. The nucleic acid extraction device of claim 12, wherein the heating element is an electrothermal film attached to the end face of the test tube holder (32).
14. The nucleic acid extraction device according to claim 12, wherein the heating module (5) further includes a temperature sensor, and the tube holder (32) further includes a detection hole provided between adjacent two of the tube accommodating holes, the temperature sensor being placed in the detection hole.
15. The nucleic acid extraction device according to any one of claims 1 to 11, further comprising a heat dissipation module (6), wherein the heat dissipation module (6) comprises a side heat dissipation fan (61) provided at a side of the test tube holder (32); and/or the heat dissipation module (6) further comprises a bottom surface heat dissipation fan (62) arranged on the bottom surface of the test tube fixing piece (32).
16. The nucleic acid extraction device according to claim 15, wherein the mixing module (3) comprises a housing (31), and the housing (31) is provided with a fan heat dissipation hole.
17. The nucleic acid extraction apparatus according to claim 1, further comprising a control module (4), wherein the control module (4) comprises a main controller (41) coupled to the centrifugal module (2), a sub-controller (42) coupled to the mixing module (3), and a conductive slip ring (43), wherein the conductive slip ring (43) is connected to the centrifugal tray (21) and electrically connects the main controller (41) and the sub-controller (42).
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