CN111149807A - Bactericide and preparation method thereof - Google Patents
Bactericide and preparation method thereof Download PDFInfo
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- CN111149807A CN111149807A CN202010055955.2A CN202010055955A CN111149807A CN 111149807 A CN111149807 A CN 111149807A CN 202010055955 A CN202010055955 A CN 202010055955A CN 111149807 A CN111149807 A CN 111149807A
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- Prior art keywords
- bactericide
- water
- cosolvent
- benzisothiazolin
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/80—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/02—Amines; Quaternary ammonium compounds
- A01N33/04—Nitrogen directly attached to aliphatic or cycloaliphatic carbon atoms
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a bactericide and a preparation method thereof, belonging to the technical field of bactericides, and the technical scheme is characterized in that the bactericide comprises 5-15% of 1, 2-benzisothiazolin-3-one, 1-5% of alcamines cosolvent and the balance of water according to weight percentage; the preparation method comprises the following steps: s1: adding isothiazolin-3-ketone, alcohol amine cosolvent, synergist and water into a container, and stirring until the mixture is dissolved to obtain a crude product solution; s2: the crude product solution in the step S1 is filtered to obtain the bactericide, so that the sterilizing performance of the bactericide is improved, and the processing cost of the bactericide is reduced.
Description
Technical Field
The invention relates to the field of bactericides, and particularly relates to a bactericide and a preparation method thereof.
Background
Most of water-containing compositions such as liquid cleaning agents, water-based coatings, fountain solutions for printing and the like are easy to undergo microbial decay due to the presence of water, the decay can not only damage the performance of products, but also cause health hazards to users under extreme conditions, so in order to protect the service cycle of the products, bactericides are generally added in the process of manufacturing the products, most of solvent auxiliaries in the existing bactericides are alcohols, potassium hydroxide and sodium hydroxide, the price of the alcohol solvents is high, the cost of the bactericides is increased, and potassium hydroxide and sodium hydroxide solvents are crystallized and precipitated due to the reduction of the temperature in winter, so that the bactericidal performance of the bactericides is reduced.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide the bactericide, which achieves the effects of improving the sterilizing performance of the bactericide and reducing the processing cost of the bactericide.
The technical purpose of the invention is realized by the following technical scheme:
the bactericide comprises, by weight, 5-15% of 1, 2-benzisothiazolin-3-one, 1-5% of an alcamines cosolvent, and the balance of water.
By adopting the technical scheme, the 1, 2-benzisothiazolin-3-one has the outstanding effect of inhibiting the propagation of microorganisms such as fungi, mould, bacteria and algae in an organic medium, and the 1, 2-benzisothiazolin-3-one has long-acting bactericidal power, can effectively kill various bacteria, moulds and the like, has good biodegradability, small influence on the environment, low toxicity, safe use and good water solubility. The alcohol amine is used as the cosolvent, so that the 1, 2-benzisothiazolin-3-one can be fully dissolved in water, meanwhile, the alcohol amine is organic weak base, the precipitation phenomenon cannot occur at low temperature, the sterilization performance of the bactericide is effectively ensured, and meanwhile, compared with the alcohol cosolvent, the alcohol amine cosolvent has a lower price, so that the processing cost of the bactericide is effectively reduced.
The invention is further set that the raw materials comprise 7-13% of 1, 2-benzisothiazolin-3-one, 1.5-4.5% of alcohol amine cosolvent and the balance of water according to weight percentage.
By adopting the technical scheme, when the bactericide is in the proportioning range, the sterilizing effect can be further improved, and the sterilizing performance and the stability are excellent.
The invention is further set that the raw materials comprise 10 percent of 1, 2-benzisothiazolin-3-ketone, 3 percent of alcohol amine cosolvent and the balance of water according to weight percentage.
By adopting the technical scheme, when 10% of 1, 2-benzisothiazolin-3-one, 3% of alcamines cosolvent and the balance of water are adopted, the bactericidal performance of the bactericide is good, and meanwhile, the bactericidal performance of the bactericide can be effectively improved.
The invention is further provided that the alcohol amine cosolvent selects one or two of triethanolamine and diethanolamine.
By adopting the technical scheme, the alkanolamine cosolvent has alkalescence when triethanolamine and diethanolamine are selected, can not be separated out at low temperature, can be used within the pH range of 1-10 after the triethanolamine or the diethanolamine are selected, and can also be used at the temperature of more than 70 ℃, so that the application environment is wider, the triethanolamine and the diethanolamine are low in toxicity and environment-friendly, the risk is low during transportation, and the explosion phenomenon cannot occur.
The invention is further provided that the raw material also comprises 1-5% of synergist, preferably 2-4%.
The invention is further configured that the synergist is N- (3-aminopropyl) -N-dodecyl-1, 3-propane diamine.
By adopting the technical scheme, the N- (3-aminopropyl) -N-dodecyl-1, 3-propane diamine has good stability and excellent emulsifying, dispersing, corrosion preventing and sterilizing effects, and after the synergist is added into the bactericide, the sterilizing and corrosion preventing effects of the bactericide are greatly improved, and meanwhile, the dosage of the 1, 2-benzisothiazolin-3-one can be reduced, so that the cost is reduced.
A preparation method of a bactericide comprises the following steps:
s1: adding isothiazolin-3-ketone, alcohol amine cosolvent, synergist and water into a container, and stirring until the mixture is dissolved to obtain a crude product solution;
s2: and filtering the crude solution in the step S1 to obtain the bactericide.
The bactericide is applied to the fields of water-based paint, printing ink, latex products, lubricating oil, leather and the like.
By adopting the technical scheme, the bactericide disclosed by the invention is widely applied to products with water existing such as water-based paint, ink, latex products, lubricating oil, leather and the like except products (namely daily chemical products) contacting with human skin, and has excellent sterilizing and anti-corrosion properties.
In conclusion, the invention has the following beneficial effects:
1. the 1, 2-benzisothiazolin-3-one is used with the alcoholic amine cosolvent in a matching way, so that the bactericidal performance of the bactericide is ensured, the phenomenon of precipitation of the bactericide at low temperature is effectively prevented, the bactericide can be used within the range of 0-10 degrees, and the excellent bactericidal performance of the bactericide at low temperature is ensured, in addition, the bactericide can be used within the range of pH of 1-10, and can be used at the temperature of more than 70 ℃, the use is environment-friendly, and compared with the alcoholic cosolvent, the transportation risk of the bactericide is low, and the processing cost is reduced;
2. the addition of N- (3-aminopropyl) -N-dodecyl-1, 3-propane diamine and the matching use of 1, 2-benzisothiazolin-3-one can effectively improve the bactericidal performance of the bactericide.
Detailed Description
The present invention will be described in further detail with reference to examples.
Example 1
A bactericide is prepared from (by weight) 1, 2-benzisothiazolin-3-one 5%, diethanolamine 5% and water in balance.
The preparation method comprises the following steps:
s1: adding isothiazolin-3-ketone, diethanol amine and water into a container, and stirring until the isothiazolin-3-ketone, the diethanol amine and the water are dissolved to obtain a crude product solution;
s2: and filtering the crude solution in the step S1 to obtain the bactericide.
Example 2
A bactericide is prepared from (by weight) 1, 2-benzisothiazolin-3-one 7%, diethanolamine 4.5% and water in balance.
The preparation method comprises the following steps:
s1: adding isothiazolin-3-ketone, diethanol amine and water into a container, and stirring until the isothiazolin-3-ketone, the diethanol amine and the water are dissolved to obtain a crude product solution;
s2: and filtering the crude solution in the step S1 to obtain the bactericide.
Example 3
A bactericide is prepared from (by weight) 1, 2-benzisothiazolin-3-one 11%, diethanolamine 3.2%, and water in balance.
The preparation method comprises the following steps:
s1: adding isothiazolin-3-ketone, diethanol amine and water into a container, and stirring until the isothiazolin-3-ketone, the diethanol amine and the water are dissolved to obtain a crude product solution;
s2: and filtering the crude solution in the step S1 to obtain the bactericide.
Example 4
A bactericide is prepared from (by weight) 1, 2-benzisothiazolin-3-one 13%, diethanolamine 1.5% and water in balance.
The preparation method comprises the following steps:
s1: adding isothiazolin-3-ketone, diethanol amine and water into a container, and stirring until the isothiazolin-3-ketone, the diethanol amine and the water are dissolved to obtain a crude product solution;
s2: and filtering the crude solution in the step S1 to obtain the bactericide.
Example 5
A bactericide is prepared from (by weight) 1, 2-benzisothiazolin-3-one 15%, diethanolamine 1% and water in balance.
The preparation method comprises the following steps:
s1: adding isothiazolin-3-ketone, diethanol amine and water into a container, and stirring until the isothiazolin-3-ketone, the diethanol amine and the water are dissolved to obtain a crude product solution;
s2: and filtering the crude solution in the step S1 to obtain the bactericide.
Example 6
A fungicide was different from that of example 3 in that diethanolamine was replaced with the same amount of triethanolamine.
Example 7
The difference between the bactericide and the bactericide in example 3 is that the raw material also comprises 1% of N- (3-aminopropyl) -N-dodecyl-1, 3-propane diamine.
The difference from example 3 in the production method is that N- (3-aminopropyl) -N-dodecyl-1, 3-propanediamine is added in step S1.
Example 8
The difference between the bactericide and the bactericide in example 3 is that the raw material also comprises 3% of N- (3-aminopropyl) -N-dodecyl-1, 3-propane diamine.
The difference from example 3 in the production method is that N- (3-aminopropyl) -N-dodecyl-1, 3-propanediamine is added in step S1.
Example 9
The difference between the bactericide and the bactericide in example 3 is that the raw material also comprises 5% of N- (3-aminopropyl) -N-dodecyl-1, 3-propane diamine.
The difference from example 3 in the production method is that N- (3-aminopropyl) -N-dodecyl-1, 3-propanediamine is added in step S1.
Detection of antiseptic and bacteriostatic performance
The antiseptics in examples 1 to 12 and comparative examples 1 to 4 were tested for bacteriostatic performance, and the results are shown in table 1, where the bacteriostatic ability of the bacteriostatic substance is judged according to the diameter of the zone of inhibition, and the larger the diameter of the zone of inhibition, the better the bacteriostatic effect of the surface bacteriostatic substance.
The preservatives of examples 1-12 and comparative examples 1-4 above were tested in the present invention by dilution in nutrient broth using the following species: escherichia coli (strain No. ATCC 11229), Staphylococcus aureus (strain No. ATCC 6538), Pseudomonas aeruginosa (strain No. ATCC 9027), Aspergillus niger (strain No. ATCC 16404), Candida albicans (strain No. ATCC 10231), and Aspergillus flavus (ATCC 9644), wherein the microorganism is subjected to tryptone soybean broth culture medium to prepare a bacterial suspension for later use.
Experimental methods
(1) Preparation of the bacterial suspension
Preparing corresponding culture medium, performing wet heat sterilization at 120 deg.C for 25min, pouring plate into super clean bench, cooling and solidifying, respectively selecting experimental strains under aseptic condition, inoculating to corresponding culture medium by streaking method, placing in incubator, culturing and activating, culturing strains at 37 deg.C for 24 hr, placing activated thallus Porphyrae of five kinds of bacteria in corresponding liquid culture medium, and adjusting the bacterial suspension concentration of Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans to 3.5 × 10 with sterile physiological saline6cfu/ml, Aspergillus niger and Aspergillus flavus concentration adjusted to 2.8 × 104cfu/ml, and storing in a refrigerator at 4 ℃ for later use.
(2) Preparation of test solutions
5.0g of each of the preservatives of examples 1 to 12 and comparative examples 1 to 4 was diluted with sterile phosphate buffer to give a 10% dispersion, and then diluted with the buffer to give 15. mu.g/mL, 10. mu.g/mL, and 5. mu.g/mL of each of the test solutions.
(3) Determination of zone of inhibition
Preparing a circular filter paper sheet with the diameter of 6mm by using a puncher, drying for later use after sterilization, respectively taking 10mL of test solution, immersing the filter paper sheet into the test solution, soaking for 20min, uniformly coating 0.2mL of bacterial suspension on the surface of a corresponding solid culture medium by using an aseptic coating rod, completely permeating bacterial liquid after 15min, clamping the filter paper sheet with the test solution and a control group by using aseptic tweezers, pasting the filter paper sheet on a bacterial coating flat plate, avoiding the overlapping of antibacterial rings at certain intervals between the paper sheets, repeating 3 groups of each bacterium, then culturing the bacterium for 24h, culturing the fungus for 48h, taking out and measuring the diameter of the antibacterial rings, and taking an average value for recording.
(4) In the same manner, a positive control group to which the bacterial suspension was added but the test solution was not added, and a negative control group to which the test solution was added but the bacterial suspension was not added were prepared.
TABLE 1 Bactericidal results of bactericide
From the above table, it can be seen that:
the diameters of the inhibition zones of the bactericides in the embodiments 1 to 5 are increased and then decreased, and reach the maximum value in the embodiment 3, which shows that the formula in the embodiment 3 can effectively improve the inhibition performance of the bactericides;
compared with the example 3, the diameter of the inhibition zone of the bactericide in the example 6 is basically the same as that of the inhibition zone in the example 3, which shows that the same inhibition effect can be achieved by replacing diethanolamine with triethanolamine in the same amount;
compared with the example 3, in the examples 7-9, after the N- (3-aminopropyl) -N-dodecyl-1, 3-propane diamine is added to the raw materials, the diameter of the inhibition zone of the bactericide is better than that of the inhibition zone in the example 3, which shows that the compounding of the N- (3-aminopropyl) -N-dodecyl-1, 3-propane diamine and 1, 2-benzisothiazolin-3-one can play a further synergistic role and effectively improve the bactericidal performance of the bactericide.
The present embodiment is only for explaining the present invention, and it is not limited to the present invention, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present invention.
Claims (8)
1. A fungicide, characterized by: the raw materials comprise, by weight, 5-15% of 1, 2-benzisothiazolin-3-one, 1-5% of alcohol amine cosolvent and the balance of water.
2. A bactericide as claimed in claim 1, wherein: the raw materials comprise, by weight, 7-13% of 1, 2-benzisothiazolin-3-one, 1.5-4.5% of alcohol amine cosolvent and the balance of water.
3. A bactericide as claimed in claim 1, wherein: the raw materials comprise, by weight, 10% of 1, 2-benzisothiazolin-3-one, 3% of an alcamines cosolvent and the balance of water.
4. A bactericide as claimed in any of claims 1 to 3, characterized in that: the alcohol amine cosolvent is one or two of triethanolamine and diethanolamine.
5. A bactericide as claimed in any of claims 1 to 3, characterized in that: the raw materials also comprise 1-5% of a synergist.
6. A bactericide as claimed in claim 5, wherein: the synergist adopts N- (3-aminopropyl) -N-dodecyl-1, 3-propane diamine.
7. The preparation method of the bactericide is characterized by comprising the following steps: mixing the ratio of 1, 2-benzo S1: adding isothiazolin-3-ketone, alcohol amine cosolvent, synergist and water into a container, and stirring until the mixture is dissolved to obtain a crude product solution;
s2: and filtering the crude solution in the step S1 to obtain the bactericide.
8. The bactericide is applied to the fields of water-based paint, printing ink, latex products, lubricating oil, leather and the like.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010055955.2A CN111149807A (en) | 2020-01-18 | 2020-01-18 | Bactericide and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010055955.2A CN111149807A (en) | 2020-01-18 | 2020-01-18 | Bactericide and preparation method thereof |
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| Publication Number | Publication Date |
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| CN111149807A true CN111149807A (en) | 2020-05-15 |
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| CN202010055955.2A Pending CN111149807A (en) | 2020-01-18 | 2020-01-18 | Bactericide and preparation method thereof |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101528043A (en) * | 2006-09-08 | 2009-09-09 | 奥麒木材保护有限公司 | Antibacterial composition containing isothiazolin-3-one |
| CN101631457A (en) * | 2007-01-12 | 2010-01-20 | 安格斯化学公司 | The amino alcohol and the Biocidal composition that are used for aqueous based systems |
| CN102559421A (en) * | 2011-12-23 | 2012-07-11 | 广州立白企业集团有限公司 | Enzyme-containing fabric detergent with synergistic anticorrosion effect |
| CN103619174A (en) * | 2011-05-13 | 2014-03-05 | Isp投资公司 | Aqueous solutions of 1,2-benzisothiazolin-3-one |
-
2020
- 2020-01-18 CN CN202010055955.2A patent/CN111149807A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101528043A (en) * | 2006-09-08 | 2009-09-09 | 奥麒木材保护有限公司 | Antibacterial composition containing isothiazolin-3-one |
| CN101631457A (en) * | 2007-01-12 | 2010-01-20 | 安格斯化学公司 | The amino alcohol and the Biocidal composition that are used for aqueous based systems |
| CN103619174A (en) * | 2011-05-13 | 2014-03-05 | Isp投资公司 | Aqueous solutions of 1,2-benzisothiazolin-3-one |
| CN102559421A (en) * | 2011-12-23 | 2012-07-11 | 广州立白企业集团有限公司 | Enzyme-containing fabric detergent with synergistic anticorrosion effect |
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Application publication date: 20200515 |
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