CN110840785B

CN110840785B - Cosmetic composition for improving skin aging or skin wrinkles

Info

Publication number: CN110840785B
Application number: CN201910307168.XA
Authority: CN
Inventors: 庆瑞娟; 李敬银; 吕炫周; 姜承贤; 朴明三
Original assignee: Cosmax Inc
Current assignee: Cosmax Inc
Priority date: 2018-08-20
Filing date: 2019-04-17
Publication date: 2022-11-22
Anticipated expiration: 2039-04-17
Also published as: KR20200021180A; KR102124698B1; CN110840785A

Abstract

The present invention relates to a cosmetic composition for skin aging or skin wrinkle improvement comprising a mixed extract of chrysanthemum, aloe, and ivy as an effective ingredient, and more particularly, to a composition for skin external preparation comprising an extract effective for improving skin wrinkles by inhibiting skin aging caused by ultraviolet rays. According to the present invention, a cosmetic composition comprising a mixed extract of chrysanthemum, aloe, and ivy as an active ingredient can exhibit skin aging prevention or wrinkle improvement effects by increasing the expression of COL1A1, which is a collagen biosynthesis gene, and inhibiting the expression of MMP-1, which is a collagen-degrading enzyme.

Description

Cosmetic composition for improving skin aging or skin wrinkles

Technical Field

The present invention relates to a cosmetic composition for skin aging or skin wrinkle improvement comprising a mixed extract of chrysanthemum, aloe, and ivy as an effective ingredient, and more particularly, to a composition for skin external preparation comprising an extract effective for wrinkle improvement by inhibiting skin aging caused by ultraviolet rays.

Background

The skin, which is the outermost organ in the body, protects our body from external environments and irritation. The skin is exposed to external irritants at all times as compared with many organs in the body, and thus may be easily attacked, and in particular, the facial skin is exposed directly to ultraviolet rays and pollutants, and thus has a characteristic of being easily aged as compared with other body skins.

Skin aging is classified into intrinsic aging occurring due to genetic factors and extrinsic aging occurring due to environmental exposure. Intrinsic aging occurs naturally as a result of biological metabolic processes, whereas extrinsic aging occurs as a result of continuous exposure, stress, smoking, and the like. In addition, from the clinical viewpoint, although the skin is dry and pale with loss of elasticity and shows fine lines due to intrinsic aging, deep wrinkles due to extrinsic aging are observed, and spots are generated and colored due to irregular pigmentation, rough skin and the like are observed, and in severe cases, diseases such as moles, black spots, skin cancer and the like are caused, and thus the skin is affected more. Therefore, in order to prevent skin aging, it is more effective to block such extrinsic aging in advance and suppress the progression of extrinsic aging.

From a histological point of view, skin aging is caused by changes in the composition of the Extracellular tissue (Extracellular matrix proteins) of the skin. In the dermal extracellular tissue, various collagens account for 90% of the dermal layer of the skin, and impart strength and tension to external stimuli or forces to protect the skin. Therefore, the reduction of collagen is closely related to skin aging and wrinkle formation. One of the main cross-linked constituents of healthy dermis is type i collagen, the precursor of which is significantly reduced in skin damaged by photoaging. When aging progresses, collagen is also decomposed, but biosynthesis of various collagen-decomposing enzymes (Matrix Metalloproteinases (MMPs)) is increased by stimulation such as ultraviolet rays to reduce collagen synthesis, thereby forming wrinkles. Various kinds of collagen degrading enzymes (MMPs) are enzymes that decompose and recombine components of the extracellular structure of dermis as intracellular proteases, and among them, matrix metalloproteinase-1 (MMP-1) acts on collagen in dermis to fractionate collagen. Therefore, the amount of expression and the degree of activity of this enzyme in vivo play an important role in the generation or amelioration of wrinkles.

Meanwhile, as a raw material for wrinkle improvement, vitamin C, α -tocopherol, retinol and derivatives thereof, etc. are mainly used in the form of being blended in cosmetics and pharmaceuticals, but these have disadvantages of generating a bad odor phenomenon or having poor chemical stability when blended in a dosage form.

In order to overcome such drawbacks, recently, development of wrinkle-improving cosmetic compositions using these natural product-derived substances has been attracting attention, and it is necessary to discover such wrinkle-improving cosmetic compositions.

Chrysanthemum (Chrysanthemum morifolium) is a flower that is generally widely used for ornamental purposes, but it is known that Chrysanthemum is used as a medicine for eyes in china and contains various vitamin precursors and minerals and active ingredients such as β -carotene that is a pigment having good antioxidant ability. Aloe Vera (Aloe Vera) is a plant used for years as a medicinal or nutritional purpose as a succulent plant growing in tropical regions. Not only for healing purposes, the gel contained in aloe leaves is often used in the cosmetic industry as cream and lotion, etc. In particular, it has been reported that vitamins, minerals, polyphenol antioxidants and the like are contained in many cases. Caulis Hederae sinensis (Hedera Helix) as a plant belonging to Araliaceae family is decocted to a concentrated state in the absence of white sugar to be used as sweetener, and stem and fruit are used as medicine in folk therapy. In particular, it is known that it has an effect of lowering blood sugar level in diabetes and has an effect on arthritis, muscle pain, cough, and the like. However, when chrysanthemum, aloe, or ivy is used, the skin aging prevention or wrinkle improvement effect has not been revealed.

In view of the above, the present inventors have conducted intensive studies in order to overcome the various problems of the prior art as described above, and finally have confirmed that a mixed extract of chrysanthemum, aloe, and ivy has superior efficacy in skin aging and wrinkle improvement than a case where a single extract is contained as an active ingredient, and have completed the present invention.

Documents of the prior art

Patent literature

(patent document 1) Korean laid-open patent No. 10-2017-0027685

(patent document 2) Korean patent laid-open No. 10-2008-63267

(patent document 3) Korean laid-open patent No. 10-2012-0081309

Disclosure of Invention

The main object of the present invention is to provide a wrinkle-improving cosmetic composition comprising a mixed extract of chrysanthemum, aloe and ivy, which is effective in improving skin aging and wrinkle formation.

According to one embodiment of the present invention, there is provided a cosmetic composition for skin aging or skin wrinkle improvement comprising a mixed extract of chrysanthemum, aloe, and caulis hederae chamomillae as an active ingredient.

The present invention is characterized in that the skin aging is caused by external factors, more specifically, the skin aging is skin photoaging caused by ultraviolet rays.

The present invention is characterized in that the extract can be extracted with any solvent conventionally used, preferably with water, an organic solvent or a mixed solvent thereof, and more preferably with at least one solvent selected from the group consisting of water, anhydrous or hydrous methanol, ethanol, propanol, butanol, glycerol, propylene glycol, butylene glycol or a mixed solvent thereof.

The present invention is characterized in that the mixed extract can be mixed in a ratio of 1-5:1-5:1-5, extracting chrysanthemum in the following mixing weight ratio: aloe vera: the hedera helix extract, preferably, can be present in a ratio of 1-3:1-3:1-3, more preferably, can be extracted in a weight ratio of 1:1:1, mixing the chrysanthemum in the mixing weight ratio: aloe vera: caulis Hederae sinensis extract. The mixed extract of the present invention may be extracted together after mixing each natural product, or may be mixed after separately extracting. The present invention is characterized in that the mixed extract can be extracted from a part of chrysanthemum, aloe, and ivy, but preferably, a flower, leaf, or stem thereof.

The invention is characterized in that the extract is contained in an amount of 0.001 to 30 weight percent based on the total weight of the composition. When the content is less than the above range, the effectiveness may be lowered, and when the content is more than the above range, the formulation may be difficult, which is not preferable.

The present invention is characterized in that the above composition has the effects of promoting the gene expression of type I collagen (COL 1A 1), inhibiting the gene expression of MMP-1 increased by ultraviolet rays to inhibit aging, and improving wrinkles.

According to the experimental examples of the present invention, it was confirmed that when human fibroblasts were treated with the mixed extract of chrysanthemum, aloe and ivy, the synergistic effect of increasing the gene expression of COL1A1 was exhibited, as compared to the case of treating the extract of chrysanthemum, aloe or ivy alone. Furthermore, it was confirmed that, in fibroblasts irradiated with ultraviolet light, when a mixed extract of chrysanthemum, aloe and caulis hederae, a synergistic effect was exhibited when the expression of MMP-1 was suppressed, as compared with the case where the extract of chrysanthemum, aloe and caulis hederae is treated alone. From these results, it was found that the mixed extract of chrysanthemum, aloe and ivy of the present invention promotes the expression of type i procollagen gene, and shows skin aging-inhibiting and wrinkle-improving effects by inhibiting the increase of MMP-1 gene expression caused by ultraviolet rays.

The present invention is characterized in that the composition can be prepared using any formulation generally prepared in this field, and preferably, the composition is one or more formulations selected from the group consisting of a skin lotion, a skin softener, a toner, an astringent, a lotion, an emulsion, a moisturizing lotion, a nourishing lotion, a massage cream, a nourishing cream, a moisturizing cream, a hand cream, a foundation, an essence, a nourishing essence, a mask, a soap, a face cleanser, a cleansing lotion, a cleansing cream, a body lotion, and a body lotion.

The cosmetic composition of the present invention may contain, as an active ingredient, in addition to the mixed extract, a water-containing aqueous phase ingredient, an oil phase ingredient containing oil or fat, an organic or inorganic powder, a surfactant, a humectant, a thickener, etc., which are generally used in cosmetic compositions, in different formulations, and may further contain, as other auxiliary ingredients, a preservative, an antioxidant, a pH adjuster, a pigment, a perfume, etc.

According to the present invention, the cosmetic composition comprising the mixed extract of chrysanthemum, aloe and ivy as an active ingredient can increase the expression of COL1A1, which is a collagen biosynthesis gene, and exhibit skin aging prevention or wrinkle improvement effects by inhibiting the expression of MMP-1, which is a collagen-degrading enzyme.

Drawings

FIG. 1 is a result of Experimental example 1 in which the effect of mixed extracts of Chrysanthemum morifolium, aloe Vera and hedera helix on the expression of mRNA of COL1A1 in human fibroblasts was confirmed.

Mixture of chrysanthemum, aloe and hedera helix 1: 3.3% of chrysanthemum, 3.3% of aloe and 3.3% of hedera helix (ul/ml); mixture of chrysanthemum, aloe and common ivy 2: chrysanthemum 5+ aloe 2.5+ common ivy 2.5 (ul/ml); mixture of chrysanthemum, aloe and ivy 3: 2.5% of chrysanthemum, 5% of aloe and 2.5% of ivy (ul/ml). ^* p<0.05, ^** p<0.01 (comparison with blank group)

FIG. 2 is a result of experimental example 2 confirming the effect of mixed extracts of Chrysanthemum morifolium Mill, aloe vera and Hedera helix on mRNA expression of MMP-1 in human fibroblasts.

Mixture of chrysanthemum, aloe and common ivy 1: 0.33% of chrysanthemum, 0.33% of aloe, 0.33% of common ivy, and 0.33 (ul/ml); mixture of chrysanthemum, aloe and common ivy 2: chrysanthemum 0.5+ aloe 0.25+ hedera helix 0.25 (ul/ml); chrysanthemum, aloe and common ivy stem mixture 3: 0.25% of chrysanthemum, 0.5% of aloe and 0.25% of common ivy (ul/ml). ^** p<0.01 (compare with blank set) and, ⁺⁺ p<0.01 (comparison with UV control group)

Detailed Description

Hereinafter, the present invention will be described in detail by way of examples and experimental examples. However, the examples and experimental examples of the present invention may be modified in various other ways, and the scope of the present invention should not be construed as being limited to the examples and experimental examples described in detail below. Examples and experimental examples of the present invention are provided to enable those skilled in the art to more fully understand the present invention.

An embodiment of the present invention provides a cosmetic composition for preventing skin aging or improving wrinkles, comprising a mixture of extracts of chrysanthemum, aloe, and hedera helix as effective ingredients. The extracts of chrysanthemum, aloe, and ivy used in the present invention were supplied from skin laboratory (Derma Lab) to be used in the experiment. The method for obtaining the above chrysanthemum, aloe and common ivy extracts is not particularly limited, and extracts prepared or sold by extraction with an organic solvent or water may be used.

The cosmetic composition is effective in improving skin aging caused by external or internal factors. The external factors are various external factors such as ultraviolet rays, while the internal factors are also called chronological factors, and mainly refer to factors generated with the passage of time.

Specifically, the cosmetic composition of the present invention comprising mixed extracts of chrysanthemum, aloe and ivy has the effect of inducing the expression of collagen biosynthesis genes to restore damaged dermis, and the effect thereof exhibits synergistic efficacy compared to the case of treating chrysanthemum, aloe or ivy alone.

Also, the cosmetic composition of the present invention can reduce collagen catabolic enzyme genes to exhibit wrinkle improvement and aging prevention effects, and the effects thereof exhibit synergistic effects as compared to the case of treating chrysanthemum, aloe, or ivy alone.

The present invention will be described in more detail below with reference to examples. However, these examples are provided to illustrate the present invention, and the scope of the present invention is not limited to these examples.

Comparative example 1: separate extracts of chrysanthemum, aloe and common ivy

After powdering chrysanthemum (flower), aloe (leaf), and hedera helix (leaf), each 100g of the powder was extracted, and each was put into a separate container as an extraction solvent, and the extract was immersed in 70 wt% of 1L of an aqueous ethanol solution and allowed to stand at room temperature for 24 hours, thereby obtaining an extract. The obtained extract was filtered with filter paper, and the filtrate was concentrated under reduced pressure with a rotary evaporation dryer to obtain extracts of 50g of chrysanthemum extract, 50g of aloe extract, and 50g of hedera helix extract.

Example 1: preparation of mixed extract of flos Chrysanthemi, aloe and caulis Hederae sinensis

As shown in the following table 1, the individual extracts of chrysanthemum, aloe, and caulis hederae chamomillae obtained in the above comparative example 1 were mixed to obtain a mixed extract.

TABLE 1

Experimental example 1: type I collagen gene expression induction effect of mixed extract of chrysanthemum, aloe and common ivy Measurement of (2)

In order to determine the type i collagen gene expression inducing effect of the mixed extracts of chrysanthemum, aloe and ivy, the corresponding extracts were provided from the skin laboratory and used in the following experiments.

Specifically, in a Human fibroblast cell line (Human dermal fibroplast, hs 68), an experiment was performed under the following conditions. First, at 3.5x10 ⁵ Hs68 fibroblasts were dispensed into 6-well plates and then, at 37 ℃ in 5% CO ₂ The culture was incubated in the conditioned medium for 24 hours. Then, the medium was removed, and the resulting mixture was washed with phosphate buffered saline (DPBS) and replaced with Fetal Bovine Serum (FBS) -free medium, and then the mixture was divided into a group in which 1% (10 ul/ml) of the chrysanthemum, aloe, and hedera helix extracts were treated alone and a group in which the final mixed concentration of the mixed extracts of chrysanthemum, aloe, and hedera helix was treated to 10ul/ml, and further cultured for 24 hours. Then, ribonucleic acid was separated from the cells of each sample by TRIZOL (RNA iso, DA KARA, japan), and then quantified by nanodrop at 260nm, and complementary deoxyribonucleic acid (cDNA) was synthesized in an amplifier using 2. Mu.g of ribonucleic acid (C1000 thermocycler, bio-Rad, USA). The real-time polymerase chain reaction was carried out in a real-time Polymerase Chain Reaction (PCR) machine using a mixture in which COL1A1 primer as a target gene and SYBR Green (supermis, applied Biosystems, usa) as a cyanine dye were added to the synthesized complementary deoxyribonucleic acid, thereby finally confirming the expression degree of COL1A1 gene. The expression level of the gene was finally analyzed by correcting the β actin (β -actin) gene, and the analysis results are shown in fig. 1.

As shown in fig. 1, when chrysanthemum, aloe, and an extract of hedera helix were treated alone, COL1A1 expression was significantly increased in chrysanthemum and aloe, but the expression increase pattern was not exhibited in the extract of hedera helix. In contrast, it was confirmed that COL1A1 expression was increased at a very prominent level when the mixed extract of chrysanthemum, aloe, and ivy was treated.

Experimental example 2: evaluation of MMP-1 expression inhibitory ability of Chrysanthemum, aloe and caulis Erythrinae mixed extract

In order to evaluate the synergistic effect of inhibiting the expression of MMP-1, which was overexpressed in mixed extracts of chrysanthemum, aloe, and ivy due to ultraviolet rays, the ability of inhibiting the expression of MMP-1 in a Human fibroblast cell line (Hs 68) was confirmed.

First, at 3.5x10 ⁵ Hs68 cells were dispensed into 6-well plates, and then, 5% of CO was measured at 37 ℃ ₂ The culture was incubated in the conditioned medium for 24 hours. Thereafter, the medium was removed and, after placing in a phosphate buffer solution, irradiated at 12mJ/cm in the remaining cell group except for the outdoor Ultraviolet (UVB) non-irradiated group ² After outdoor uv, the medium was replaced with fetal calf serum free medium. Then, samples were treated in a group in which 0.1% (1 ul/ml) of the extracts of chrysanthemum, aloe and hedera helix were treated alone and in a group in which the mixed extracts of chrysanthemum, aloe and hedera helix were treated so that the final mixed concentration became 1ul/ml, and then cultured for 24 hours. Then, ribonucleic acid was separated from the cells of each sample by TRIZOL (RNA iso, DAKARA, japan), and then quantified at 260nm by nanodrop, and complementary deoxyribonucleic acid was synthesized in an amplifier using 2. Mu.g of ribonucleic acid (C1000 thermocycler, bio-Rad, USA). The real-time polymerase chain reaction was performed in a real-time polymerase chain reaction machine using a mixture in which MMP-1 primer as a target gene and SYBR Green (supermis, applied biosystems, usa) as a cyanine dye were added to the synthesized complementary deoxyribonucleic acid, thereby finally confirming the expression degree of COL1A1 gene. The expression level of the gene was finally analyzed by correcting the beta actin gene, and the gene was analyzedThe analysis results are shown in FIG. 2.

As shown in FIG. 2, the MMP-1 expression level was significantly increased in the outdoor UV irradiation group. On the other hand, when chrysanthemum, aloe and caulis ampelopsis grossedentata extracts are treated separately, MMP-1 is inhibited, and when chrysanthemum, aloe and caulis ampelopsis grossedentata extracts are treated in combination, a more prominent inhibitory effect on the expression level is exhibited, and thus a synergistic effect on MMP-1 expression inhibition can be confirmed.

Dosage form example 1: preparation of skin-refreshing agent

A skin-refreshing agent comprising mixed extracts of chrysanthemum, aloe and hedera helix was prepared in a usual manner according to the composition components and composition ratios shown in the following table 2.

TABLE 2

Dosage form example 2: preparation of emulsions

An emulsion comprising mixed extracts of chrysanthemum, aloe and ivy was prepared in a usual manner according to the composition components and composition ratios shown in the following table 3.

TABLE 3

Composition (I)	Content (percentage by weight)
		(Chrysanthemum)	0.33
Aloe vera	0.33
		Caulis Hederae sinensis	0.33
Glycerol	7.0
		Butanediol	3.0
Glycerol stearate/PEG-100 stearate	1.0
		Polysorbate 60	0.5
Sorbitan sesquioleate	0.5
		Squalane	3.0
Caprylic/capric triglyceride	5.0
		Shea butter	2.0
Stearyl alcohol	0.5
		Stearic acid	0.5
Carboxyvinyl polymer	0.2
		Arginine	0.2
Preservative	Micro-scale
		Perfume	Micro-scale
Purified water	Allowance of

Dosage form example 3: preparation of cream

A cream containing a mixed extract of chrysanthemum, aloe and hedera helix was prepared in a usual manner according to the composition components and composition ratios shown in the following table 4.

TABLE 4

Composition (I)	Content (percentage by weight)
		(Chrysanthemum)	0.33
Aloe vera	0.33
		Caulis Hederae sinensis	0.33
Glycerol	7.0
		Butanediol	5.0
Glycerol stearate/PEG-100 stearate	2.0
		Polysorbate 60	0.5
Sorbitol stearate	0.5
		Plant squalane	5.0
Jojoba oil	3.0
		Caprylic/capric triglyceride	10.0
Aloe grease	3.0
		Beeswax (Cera flava)	1.0
Stearyl alcohol	0.5
		Behenyl alcohol	0.5
Stearic acid	0.5
		Carboxyvinyl polymer	0.2
Arginine	0.3
		Polydimethylsiloxane	0.5
Preservative agent	Micro-scale
		Perfume	Micro-scale
Purified water	Balance of

The above description is only for illustrating the technical idea of the present invention by way of example, and a person skilled in the art to which the present invention pertains may make various modifications and variations within a scope not departing from the essential characteristics of the present invention.

Claims

1. A cosmetic composition for improving skin aging or skin wrinkles, comprising a mixed extract of Chrysanthemum (Chrysanthemum morifolium), aloe and hedera helix as an active ingredient, wherein the extract is extracted with water, ethanol or a mixed solvent thereof, and the ratio of the water to the ethanol in the mixture is 1:1:1 weight ratio of chrysanthemum, aloe and caulis hederae chamomillae extracts.

2. The cosmetic composition for skin aging or skin wrinkle improvement according to claim 1, wherein the mixed extract of chrysanthemum, aloe and hedera helix is contained in an amount of 0.001 to 30 wt% based on the total weight of the cosmetic composition.

3. The cosmetic composition for skin aging or skin wrinkle improvement according to claim 1, wherein the mixed extract of chrysanthemum, aloe and ivy increases expression of type i collagen gene COL1A1 or inhibits expression of matrix metalloproteinase-1 (MMP-1).

4. The cosmetic composition for improving skin aging or skin wrinkles as claimed in claim 1, wherein the composition is in the form of one or more selected from the group consisting of a skin lotion, a skin softener, a toner, an astringent and a skin lotion.

5. The cosmetic composition for improving skin aging or skin wrinkles according to claim 1, wherein the composition is in the form of one or more agents selected from the group consisting of lotion, moisturizing lotion, nourishing lotion, massage cream, nourishing cream, moisturizing cream, hand cream, foundation, and essence.

6. The cosmetic composition for improving skin aging or skin wrinkles according to claim 1, wherein the composition is in the form of one or more selected from the group consisting of a nutrient essence, a mask, a soap, a facial cleanser, a skin cleansing lotion, a cleansing cream, a body lotion and a body lotion.