CN110819526A - An in vitro simulation device for intestinal biomimetic fermentation and digestion - Google Patents

Abstract

The invention discloses an in vitro simulation device for intestinal bionic fermentation and digestion, comprising a simulation box, a heater is fixedly connected to the inner bottom wall of the simulation box, a heat conduction block is fixedly connected to the upper surface of the heater, and the heat conduction block is fixedly connected to the upper surface of the heater. The upper surface of the block is fixedly connected with a heating frame, the interior of the heating frame is provided with a simulation cylinder, the upper part of the simulation box is provided with a feeding pipe, and the bottom end of the feeding pipe runs through the simulation box and the simulation cylinder and extends in turn. To the interior of the simulation cylinder, a liquid inlet pipe is arranged above the simulation box, a through port is opened on the upper surface of the simulation box, and the bottom end of the liquid inlet pipe penetrates the through hole and the simulation cylinder in turn and extends to the simulation cylinder. internal. The intestinal biomimetic fermentation and digestion in vitro simulation device can enable better fermentation and digestion of mixed materials, facilitate viewing of the simulated state of the mixed materials, and can better operate the intestinal biomimetic fermentation and digestion in vitro simulation device , to avoid the problem of complicated operation.

Description

An in vitro simulation device for intestinal biomimetic fermentation and digestion

technical field

The invention belongs to the technical field of biological experiments, in particular to an in vitro simulation device for intestinal biomimetic fermentation and digestion.

Background technique

Biological experiment refers to the process of purposefully observing and studying the structure of organisms and life activity phenomena that are not easily observed under normal circumstances through scientific methods using certain instruments, materials and drugs under specific environmental conditions. It is necessary to simulate the digestion of the gastrointestinal tract of animals, so that the feed suitable for the growth of livestock can be formulated, but the current intestinal biomimetic fermentation and digestion in vitro simulation devices are too complicated to operate, and the simulation state of gastrointestinal biomimetic fermentation is poor. , it is impossible to observe more intuitively, so to solve the above problems, we provide an in vitro simulation device for intestinal biomimetic fermentation and digestion.

SUMMARY OF THE INVENTION

The present invention is provided to solve the above-mentioned problems that the manual operation of the current intestinal biomimetic fermentation and digestion in vitro simulation device is too cumbersome, and the simulation state of the gastrointestinal biomimetic fermentation is poor and cannot be observed more intuitively.

The present invention is realized in this way, an in vitro simulation device for intestinal biomimetic fermentation and digestion, comprising a simulation box, a heater is fixedly connected to the inner bottom wall of the simulation box, and a heat conduction block is fixedly connected to the upper surface of the heater, A heating frame is fixedly connected to the upper surface of the heat-conducting block, a simulation cylinder is arranged inside the heating frame, a feeding pipe is arranged above the simulation box, and the bottom end of the feeding pipe runs through the simulation box and the simulation box in turn. The upper surface of the simulation box is provided with a liquid inlet pipe, and the bottom end of the liquid inlet pipe extends through the through hole and the simulation cylinder in turn. To the inside of the simulation cylinder, and the outer surface of the liquid inlet pipe is fixedly connected with the inner wall of the port, the inner top wall of the simulation box is fixedly connected with a gear motor, and the output end of the gear motor is fixedly connected with a rotating rod through a reducer , the upper surface of the simulated cylinder is fixedly embedded with a bearing, the bottom end of the rotating rod penetrates the bearing and extends to the interior of the simulated cylinder, and the outer surface of the rotating rod is fixedly connected with the inner ring of the bearing, and the upper The surface is fixedly connected with a relatively symmetrical dial plate.

The inner wall of the simulation cylinder is fixedly connected with a filter screen, the bottom surface of the simulation cylinder is provided with a liquid discharge port, and the bottom surface of the liquid discharge port is fixedly connected with a liquid discharge pipe, and the left end of the liquid discharge pipe penetrates the simulation box and extends To the left side of the simulation box, the outer surface of the drain pipe is fixedly connected with a valve, the interior of the simulation cylinder is provided with two discharge pipes, and the ends of the two discharge pipes away from each other pass through the simulation cylinder in turn and the simulation box and extend to the left and right sides of the simulation box, the interior of each of the discharge pipes is clamped and blocked, the front of the simulation box is fixedly inlaid with a transparent glass plate, and the front of the simulation box is fixedly connected with In the control panel, symmetrical reinforcement blocks are fixedly connected to the outer surface of the simulation cylinder, and one side of the two reinforcement blocks that are far away from each other is fixedly connected to the inner side wall of the simulation box.

A feed cover is arranged above the simulation box, and the bottom end of the feed cover is in fixed communication with the top end of the feed pipe.

A fixing block is fixedly connected to the outer surface of the feeding pipe, and the left side of the fixing block is fixedly connected to the inner side wall of the simulation box.

The inside of the liquid inlet pipe is clamped with a rubber plug, and the outer surface of the rubber plug is in contact with the inner ring of the liquid inlet pipe.

The outer surface of the reduction motor is fixedly connected with relatively symmetrical fixing plates, and the upper surface of each fixing plate is fixedly connected with the inner top wall of the simulation box.

The bottom surface of the simulation box is fixedly connected with a base, and the bottom surface of the base is provided with anti-skid lines.

Compared with the prior art, the beneficial effect of the present invention is that the provided heater can generate a certain amount of heat and heat the heating frame, so that the temperature of the water inside the heating frame can be raised. , the temperature of the mixed material inside the simulation cylinder will increase, so that the mixed material can be better fermented and digested. The geared motor will drive the rotating rod to rotate, and the rotation of the rotating rod can drive the dial The material plate moves the mixed material in the simulation cylinder to make the mixed material peristaltic, which will make the mixed material better ferment and digest, so as to facilitate the viewing of the simulated state of the mixed material. , which can facilitate the operation of the intestinal biomimetic fermentation and in vitro simulation device, and solve the problem of cumbersome operation of the intestinal biomimetic fermentation and in vitro simulation device. Digestion in vitro simulates the state of the device at the time of simulation, so that feed suitable for the growth of livestock can be formulated.

Description of drawings

Fig. 1 is the sectional view of the front view of the simulation box of the present invention;

Fig. 2 is the front view of the simulation box of the present invention;

FIG. 3 is an enlarged schematic view of the structure at A in FIG. 1 of the present invention.

In the figure: 1. Simulation box; 2. Heater; 3. Thermal block; 4. Heating frame; 5. Simulation cylinder; 6. Feeding pipe; 7. Liquid inlet; 8. Port; 9. Geared motor; 10. Bearing; 11. Rod; 12. Dispatching plate; 13. Filter screen; 14. Drainage port; 15. Drainage pipe; 16. Valve; 17. Discharge pipe; 18. Blockage; 19. Transparent glass plate; 20, control panel; 21, base; 22, feed cover; 23, fixed block; 24, reinforcement block; 25, rubber stopper; 26, fixed plate.

Detailed ways

In order to make the objectives, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention, but not to limit the present invention.

In the description of the present invention, it should be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", The orientations or positional relationships indicated by "horizontal", "top", "bottom", "inside", "outside", etc. are based on the orientations or positional relationships shown in the accompanying drawings, which are only for the convenience of describing the present invention and simplifying the description, rather than An indication or implication that the referred device or element must have a particular orientation, be constructed and operate in a particular orientation, is not to be construed as a limitation of the invention. In addition, in the description of the present invention, "plurality" means two or more, unless otherwise expressly and specifically defined.

Please refer to FIGS. 1-3 , an in vitro simulation device for intestinal biomimetic fermentation and digestion, including a simulation box 1, a heater 2 is fixedly connected to the inner bottom wall of the simulation box 1, and the heater 2 is a well-known device in the prior art. Without going into too much description, the upper surface of the heater 2 is fixedly connected with a heat-conducting block 3 , and the upper surface of the heat-conducting block 3 is fixedly connected with a heating frame 4 . There is a feeding pipe 6, and the bottom end of the feeding pipe 6 runs through the simulation box 1 and the simulation cylinder 5 in turn and extends to the interior of the simulation cylinder 5. The upper surface of the simulation box 1 is provided with a liquid inlet pipe 7, and the upper surface of the simulation box 1 is provided with There is a port 8, the bottom end of the liquid inlet pipe 7 penetrates the port 8 and the simulated cylinder 5 in turn and extends to the interior of the simulated cylinder 5, and the outer surface of the liquid inlet pipe 7 is fixedly connected with the inner wall of the port 8, and the simulated box 1 A deceleration motor 9 is fixedly connected to the inner top wall of the motor 9, the output end of the deceleration motor 9 is fixedly connected with a rotating rod 11 through a reducer, the upper surface of the simulation cylinder 5 is fixedly inlaid with a bearing 10, and the bottom end of the rotating rod 11 penetrates the bearing 10 and extends The outer surface of the rotating rod 11 is fixedly connected with the inner ring of the bearing 10 , and the upper surface of the rotating rod 11 is fixedly connected with a relatively symmetric material-removing plate 12 .

The inner wall of the simulation cylinder 5 is fixedly connected with a filter screen 13, the bottom surface of the simulation cylinder 5 is provided with a liquid discharge port 14, the bottom surface of the liquid discharge port 14 is fixedly connected with a liquid discharge pipe 15, and the left end of the liquid discharge pipe 15 penetrates the simulation box 1 and extends To the left side of the simulation box 1, a valve 16 is fixedly connected to the outer surface of the liquid discharge pipe 15, and two discharge pipes 17 are arranged inside the simulation cylinder 5. 5 and the simulation box 1 and extend to the left and right sides of the simulation box 1, the inside of each discharge pipe 17 is clamped with a block 18, the front of the simulation box 1 is fixed and inlaid with a transparent glass plate 19, and the front of the simulation box 1 is fixed A control panel 20 is connected, and a symmetrical reinforcement block 24 is fixedly connected to the outer surface of the simulation cylinder 5 .

In the present invention, a feeding cover 22 is arranged above the simulation box 1, and the bottom end of the feeding cover 22 is fixedly connected with the top of the feeding pipe 6, which facilitates the discharge of the sample feed and prevents the phenomenon of scattering. The outer surface is fixedly connected with a fixed block 23, and the left side of the fixed block 23 is fixedly connected with the inner side wall of the simulation box 1, which deepens the fixation of the feeding pipe 6 and can make the feeding pipe 6 more stable, and the liquid feeding pipe 7 A rubber plug 25 is snapped into the inside of the rubber plug 25, and the outer surface of the rubber plug 25 is in contact with the inner ring of the liquid inlet pipe 7, which can seal the liquid inlet pipe 7 and avoid the trouble caused by the entry of sundries.

The outer surface of the deceleration motor 9 is fixedly connected with a relatively symmetrical fixing plate 26, and the upper surface of each fixing plate 26 is fixedly connected with the inner top wall of the simulation box 1, which plays a role in fixing the deceleration motor 9, and can make the deceleration motor 9 fixed. 9. More stable, the bottom surface of the simulation box 1 is fixedly connected with the base 21, and the bottom surface of the base 21 is provided with anti-skid lines, which can better support the simulation box 1 and prevent sliding.

The working principle of the present invention is as follows: when the intestinal biomimetic fermentation and digestion in vitro simulation device is in the simulation experiment, firstly, the intestinal biomimetic fermentation and digestion in vitro simulation device is connected with the power supply, and then the feed sample is passed through the feeding cover 22 and the feeding The tube 6 is put into the simulated cylinder 5, and then the rubber stopper 25 is opened to discharge the gastric simulated digestive juice from the liquid inlet pipe 7 into the simulated cylinder 5, and then the control panel 20 is used to control the heater 2 to work. A certain amount of heat will be generated, and the heat will be transferred to the heating frame 4 through the heat conduction block 3, and the water source in the heating frame 4 will be heated, so as to achieve the heating of the simulation cylinder 5. Through the work of the deceleration motor 9, it will drive the The rotation of the rotating rod 11, through the rotation of the rotating rod 11, will drive the dial plate 12 to move the mixed material in the simulation cylinder 5, so that through heating and the mixing of the material, the simulation cylinder 5 can be better fermented and fermented. Digestion, the digested mixture will be discharged from the drain pipe 15, and the undigested mixture will be blocked by the filter screen 13, and then open the block 18, and then pour clean water into the feeding pipe 6, so as to make the undigested mixture The mixture is discharged from the discharge pipe 17.

The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements and improvements made within the spirit and principles of the present invention shall be included in the protection scope of the present invention. Inside.

Claims (6)

1. The utility model provides an external analogue means of bionical fermentation of intestinal and digestion, includes simulation case (1), its characterized in that: the inner bottom wall of the simulation box (1) is fixedly connected with a heater (2), the upper surface of the heater (2) is fixedly connected with a heat conduction block (3), the upper surface of the heat conduction block (3) is fixedly connected with a heating frame (4), a simulation cylinder (5) is arranged inside the heating frame (4), a feed pipe (6) is arranged above the simulation box (1), the bottom end of the feed pipe (6) sequentially penetrates through the simulation box (1) and the simulation cylinder (5) and extends to the inside of the simulation cylinder (5), a liquid inlet pipe (7) is arranged above the simulation box (1), the upper surface of the simulation box (1) is provided with an opening (8), the bottom end of the liquid inlet pipe (7) sequentially penetrates through the opening (8) and the simulation cylinder (5) and extends to the inside of the simulation cylinder (5), and the outer surface of the liquid inlet pipe (7) is fixedly connected with the inner wall of the liquid inlet (8), the simulation device is characterized in that a speed reducing motor (9) is fixedly connected to the inner top wall of the simulation box (1), the output end of the speed reducing motor (9) is fixedly connected with a rotating rod (11) through a speed reducer, a bearing (10) is fixedly embedded in the upper surface of the simulation cylinder (5), the bottom end of the rotating rod (11) penetrates through the bearing (10) and extends into the simulation cylinder (5), the outer surface of the rotating rod (11) is fixedly connected with the inner ring of the bearing (10), and symmetrical material shifting plates (12) are fixedly connected to the upper surface of the rotating rod (11);

the inner wall of the simulation cylinder (5) is fixedly connected with a filter screen (13), a liquid discharge port (14) is formed in the bottom surface of the simulation cylinder (5), a liquid discharge pipe (15) is fixedly communicated with the bottom surface of the liquid discharge port (14), the left end of the liquid discharge pipe (15) penetrates through the simulation box (1) and extends to the left side of the simulation box (1), a valve (16) is fixedly communicated with the outer surface of the liquid discharge pipe (15), two discharge pipes (17) are arranged inside the simulation cylinder (5), one ends, far away from each other, of the two discharge pipes (17) sequentially penetrate through the simulation cylinder (5) and the simulation box (1) and extend to the left side and the right side of the simulation box (1), a plug (18) is clamped inside each discharge pipe (17), a transparent glass plate (19) is fixedly embedded in the front of the simulation box (1), and a control panel (20) is fixedly connected to the front of the simulation box (1), the outer surface of the simulation cylinder (5) is fixedly connected with symmetrical reinforcing blocks (24), and two sides, far away from each other, of the reinforcing blocks (24) are fixedly connected with the inner side wall of the simulation box (1).

2. The in vitro simulation device for intestinal biomimetic fermentation and digestion according to claim 1, wherein: the top of simulation case (1) is equipped with feeding cover (22), the bottom of feeding cover (22) and the fixed intercommunication in top of inlet pipe (6).

3. The in vitro simulation device for intestinal biomimetic fermentation and digestion according to claim 1, wherein: the outer fixed surface of inlet pipe (6) is connected with fixed block (23), the left surface of fixed block (23) and the inside wall fixed connection of simulation case (1).

4. The in vitro simulation device for intestinal biomimetic fermentation and digestion according to claim 1, wherein: the inside joint of feed liquor pipe (7) has plug (25), and the surface of plug (25) contacts with the inner circle of feed liquor pipe (7).

5. The in vitro simulation device for intestinal biomimetic fermentation and digestion according to claim 1, wherein: the outer surface of the speed reducing motor (9) is fixedly connected with symmetrical fixing plates (26), and the upper surface of each fixing plate (26) is fixedly connected with the inner top wall of the simulation box (1).

6. The in vitro simulation device for intestinal biomimetic fermentation and digestion according to claim 1, wherein: the bottom surface of the simulation box (1) is fixedly connected with a base (21), and anti-skid grains are arranged on the bottom surface of the base (21).

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