CN110747206B - 3-hydroxy-3-methylglutaryl coenzyme A reductase gene RKHMGR and application thereof - Google Patents
3-hydroxy-3-methylglutaryl coenzyme A reductase gene RKHMGR and application thereof Download PDFInfo
- Publication number
- CN110747206B CN110747206B CN201911070771.7A CN201911070771A CN110747206B CN 110747206 B CN110747206 B CN 110747206B CN 201911070771 A CN201911070771 A CN 201911070771A CN 110747206 B CN110747206 B CN 110747206B
- Authority
- CN
- China
- Prior art keywords
- ala
- leu
- ser
- val
- gly
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01034—Hydroxymethylglutaryl-CoA reductase (NADPH) (1.1.1.34)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Mycology (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a 3-hydroxy-3-methylglutaryl coenzyme A reductase gene RKHMGR and application thereof, wherein the nucleotide sequence is shown as SEQ ID NO:1, the amino acid sequence coded by the gene is shown as SEQ ID NO: 2, the gene is rhodosporidium toruloides (Rhodosporidium kratochvilovae) The key enzyme gene for synthesizing carotenoid in YM25235 is the first rate-limiting enzyme in MVA pathway and can regulate and control Rhodosporidium toruloides (Zymomonas mobilis)Rhodosporidium kratochvilovae) YM25235 produces carotenoids; the invention improves the yield of carotenoid in the microorganism by modifying the microorganism by means of genetic engineering, and lays a foundation for large-scale commercial production of the carotenoid.
Description
Technical Field
The invention belongs to the field of biotechnology and genetic engineering, relates to a 3-hydroxy-3-methylglutaryl coenzyme A reductase gene RKHMGR, and in particular relates to a yeast-rhodosporidium toruloides (yeast)Rhodosporidium kratochvilovae) The 3-hydroxy-3-methylglutaryl coenzyme A reductase gene RKHMGR cloned in YM25235 and the gene are directly connected with different vectors and transferred into yeast cells to improve the expression level of the gene and finally promote the synthesis of carotenoid.
Background
Carotenoids (carotenoids) are a class of fat-soluble pigments, and many studies have found that carotenoids are widely present in plants, animals, and microorganisms, generally in yellow, orange-red, or purple colors. Carotenoids are terpenoids consisting of an isoprene skeleton, mostly a forty-carbon molecular skeleton formed by two twenty-carbon unit tails connected to a tail, and from which a number of different compounds can be derived. Natural carotenoids have been found to date in more than about 700 kinds, and can be classified into two major groups, one being carotene (containing only two elements of carbon and hydrogen) and the other being lutein (containing oxygen-containing functional groups such as hydroxyl, keto, carboxyl, and methoxy groups), depending on the presence or absence of oxygen. They can also be classified into monocyclic carotenoids, bicyclic carotenoids and chain carotenoids according to their molecular structures. The most common carotenoids are beta-carotene, lycopene, astaxanthin.
Natural carotenoids have very strong oxidation resistance, and can scavenge free radicals, prevent aging, prevent cancer and the like, so that the natural carotenoids are widely applied to various fields of food, pharmacy, feed, cosmetics and the like as novel antioxidant active substances in pharmaceutical raw materials, dietary supplements and functional foods.
With the increasing demand for natural carotenoid preparations, the production and quality of carotenoids are still not meeting the market needs at present, although there have been considerable research by many researchers on how to increase the production of carotenoids. At present, the production methods of carotenoids mainly include plant extraction methods, chemical synthesis methods and biological synthesis methods. However, since chemically synthesized carotenoids are not healthy and cause environmental pollution, the yield of carotenoids directly extracted from natural plant raw materials is limited by the extraction method, and thus, the production of safe and environment-friendly carotenoids by fermentation using microorganisms instead of chemically synthesized carotenoids is highly advantageous. At present, the microorganisms capable of producing carotenoids by fermentation mainly comprise fungi, bacteria, yeasts and the like, and the research of producing carotenoids by yeast fermentation is the most common.
Carotenoids are mainly synthesized in yeast cells through a Mevalonate (MVA) pathway, isoprenyl pyrophosphate (IPP) is synthesized through a mevalonate pathway by taking acetyl coenzyme A as a raw material, the IPP forms dimethylallyl pyrophosphate (DMAPP) through an isomerase action, the DMAPP is condensed with three IPPs to sequentially form geranyl pyrophosphate (GPP), farnesyl pyrophosphate (FPP) and geranylgeranyl pyrophosphate (GGPP), and the GGPP forms beta-carotene, gamma-carotene, rhodotorula, rhodotorubin and astaxanthin through a series of reactions such as condensation, dehydrogenation and cyclization under the action of the enzyme. RKHMGR is a rate-limiting enzyme in MVA approach, 3-hydroxy-3-methylglutaryl coenzyme A reductase gene RKHMGR is researched, the influence of RKHMGR on a synthesis mechanism of carotenoid produced by yeast is analyzed, and a foundation is laid for large-scale production of the carotenoid.
Disclosure of Invention
The invention aims to provide a 3-hydroxy-3-methylglutaryl coenzyme A reductase gene RKHMGR, which is prepared from red winter sporulation yeast (A)Rhodosporidium kratochvilovae) YM25235, the nucleotide sequence of the gene is shown in SEQ ID NO:1 or the fragment of the nucleotide sequence, or the nucleotide sequence complementary to SEQ ID NO:1, the length of the gene sequence is 3981bp (basic group), and the amino acid sequence coded by the gene is shown in SEQ ID NO: 2 or a fragment thereof.
Another objective of the invention is to provide a recombinant expression vector containing the 3-hydroxy-3-methylglutaryl coenzyme A reductase gene RKHMGR, which is constructed by directly connecting the nucleotide sequence shown in SEQ ID NO:1 with different expression vectors (plasmids, viruses or carriers). The nucleotide sequence of the 3-hydroxy-3-methylglutaryl-CoA reductase gene RKHMGR and the appropriate transcription/translation regulatory elements can be constructed using methods well known to the person skilled in the art; these methods include in vitro recombinant DNA techniques, DNA synthesis techniques, in vivo recombinant techniques, and the like.
The DNA fragment sequence of the present invention can be obtained by the following method: (1) isolating double-stranded DNA sequences from genomic DNA; (2) chemically synthesizing a DNA sequence to obtain a double-stranded DNA of the polypeptide; the nucleotide sequence of the 3-hydroxy-3-methylglutaryl-CoA reductase gene RKHMGR is then operably linked to the appropriate promoter of the expression vector to direct mRNA synthesis. Representative examples of such promoters are: lac or trp promoter of E.coli; the PL promoter of lambda phage; eukaryotic promoters include CMV early promoter, HSV thymidine kinase promoter, early and late SV40 promoter, LTRs of retrovirus, and other known promoters capable of controlling the expression of genes in prokaryotic or eukaryotic cells or viruses. The expression vector also includes a ribosome binding site for translation initiation, a transcription terminator, and the like. The insertion of enhancer sequences into vectors will enhance transcription in higher eukaryotic cells. Enhancers are cis-acting elements of DNA expression, usually about 10-300bp, that act on a promoter to enhance gene transcription, such as adenovirus enhancers.
Another object of the present invention is to provide a host cell containing the 3-hydroxy-3-methylglutaryl coenzyme A reductase gene RKHMGR or the recombinant expression vector described above.
Another object of the present invention is to use the above-mentioned 3-hydroxy-3-methylglutaryl coenzyme A reductase gene RKHMGR in promoting the production of carotenoids by microorganisms.
The invention relates to a method for preparing a red wintergreen spore yeast (Rhodosporidium toruloides)Rhodosporidium kratochvilovae) Separating 3-hydroxy-3-methylglutaryl coenzyme A reductase gene RKHMGR from the total RNA gene of YM25235, wherein the total length of the gene is 3981 bp; overexpression of RKHMGR gene in Rhodosporidium toruloides YM25235 can cause the transcription level of the gene in cells to be improved to a certain extent, which indicates that exogenous genes are transcribed in thalli and then translated into corresponding proteins, and causes the expression level of enzymes related to carotenoid synthesis in cells to be improved. The research result is helpful to elucidate the carotenoid production mechanism in the rhodosporidium toruloides YM25235, provide reference for disclosing the mechanism for improving the microbial carotenoid production, improve the carotenoid content by modifying the rhodosporidium toruloides YM25235 through a genetic engineering means, and realize the industrial production of the carotenoidProvides good application prospect and economic benefit, and lays a foundation for large-scale commercial production of carotenoid.
Drawings
FIG. 1 is a diagram showing PCR amplification of RKHMGR gene from Rhodosporidium toruloides YM25235 of the present invention;
FIG. 2 is a plasmid map of recombinant plasmid pRHRKHMGR;
FIG. 3 shows restriction analysis of the recombinant plasmid pRHRKHMGR; wherein: 1.DNA molecular weight marker DL 10000; 2. carrying out double digestion on BamH I and EcoR V of the plasmid pRH 2034; 3. carrying out double digestion on BamH I and EcoR V of the recombinant plasmid pRHRKHMGR; 4. PCR products of RKHMGR gene; 5.DNA molecular weight marker DL 5000;
FIG. 4 shows the verification of positive clone of recombinant plasmid pRHRKHMGR transformed Rhodosporidium toruloides YM 25235; DNA molecular scalar DL 5000; 2. primers RKHMGR-F and RKHMGR-R PCR products amplified with YM25235 genome; 3. primers RKHMGR-F and RKHMGR-R PCR products amplified with YM25235/RKHMGR strain genome; 4. primers RKHMGR-F and RKHMGR-R PCR product of plasmid pRHRKHMGR; DNA molecular scalar DL 5000;
FIG. 5 shows the results of the total carotenoid content of the over-expressed strain YM25235/pRHRKHMGR and the control strain YM 25235.
Detailed Description
The present invention is further illustrated in detail below with reference to the drawings and examples, but the scope of the present invention is not limited to the above description, and reagents and methods used in the examples are, unless otherwise specified, conventional reagents and conventional methods.
Example 1: from Rhodosporidium toruloides (A)Rhodosporidium kratochvilovae) Nucleotide sequence of isolated 3-hydroxy-3-methylglutaryl coenzyme A reductase gene RKHMGR from YM25235
Extracting total RNA of Rhodosporidium toruloides YM25235 by using UNlQ-10 column type Trizol total RNA extraction Kit (product number: SK 1321) of Shanghai bioengineering (Shanghai) GmbH, carrying out reverse transcription synthesis of cDNA according to TaKaRa Kit PrimeScript RT reagentkit With gDNA Eraser (Perfect read Time), carrying out polymerase chain reaction by taking 0.5 mu L as a template, designing specific primers RKHMGR-F and RKHMGR-R (primer 1 and primer 2) according to the RKHMGR sequence found in transcriptome sequencing, carrying out PCR amplification on the obtained cDNA template on a PCR instrument (BIOER company), wherein the primers, the reaction system and the amplification conditions are as follows:
primer 1: RKHMGR-F: 5' -TTCGGATCCTTATGGTCCTCTCGCCGTC-3’（SEQ ID NO:3）
Primer 2: RKHMGR-R: 5' -GCAGATATCTTACTCTTTGGCGAACCCG-3’（SEQ ID NO:4）
（ GGATCC Is composed ofBamH IThe site of the enzyme is cut by the enzyme,GATATCis composed ofEcoRV cleavage site);
amplification conditions: pre-denaturing at 94 ℃ for 5 min, then denaturing at 94 ℃ for 30 s, annealing at 63 ℃ for 30 s, extending at 72 ℃ for 4min, performing 30 cycles, finally completely extending at 72 ℃ for 10min, taking 2 mu L of product after reaction, performing electrophoresis analysis in 1% agarose gel, obtaining a fragment with the size of about 4000bp by amplification, recovering the fragment by using an agarose gel DNA recovery kit (Beijing Solebao scientific and technology Co., Ltd.), connecting the recovered fragment to pMD-18T (TaKaRa product), and transforming the connecting product to CaCl2Escherichia coli DH 5. alpha. treated by the method was cultured overnight on LB solid plate containing ampicillin (100. mu.g/mL), white colonies growing on the plate were picked, and positive clones were verified by colony PCR. The positive clones were inoculated into LB liquid medium (containing 100 μ g/mL ampicillin) for overnight culture, plasmids were extracted with a high purity plasmid miniprep kit (centrifugal column type) (Beijing Baitaike Biotechnology, Inc.), and the amplified fragments were 3981bp in size by sequencing (Kunming Shuzo Biotechnology, Inc.), named as HMRKGR, and the sequence composition was the nucleotide sequence shown in SEQ ID NO: 1.
Example 2: construction of overexpression vector pRHRHMGR
Reverse transcribed YM25235 cDNA as template with RKHMThe GR-F and RKHMGR-R are used as primers to amplify the coding sequence of RKHMGR, the size of the obtained RKHMGR fragment is about 4000bp, and the RKHMGR fragment obtained by amplification is subjected to amplificationBamH I、EcoPerforming enzyme digestion on two restriction enzymes of RV, and connecting to an expression vector pRH2034 to obtain a recombinant plasmid pRHRKHMGR (figure 2); transferring the obtained recombinant plasmid into Escherichia coli DH5 alpha for amplification, performing colony PCR verification, extracting recombinant plasmid, and purifying withBamH I、EcoPerforming double enzyme digestion verification on pRHRKHMGR by RV; the results showed that the recombinant plasmid pRHRKHMGR generated two bands of about 4kb and 10.7 kb after double digestion (FIG. 3, lane 3), which were identical in size to the RKHMGR fragment and the pRH2034 vector after double digestion, and the success of the recombinant plasmid pRHRKHMGR construction was preliminarily demonstrated. Sequencing by using a sequencing primer, and sending out the plasmid with correct enzyme digestion verification for further verification; the sequencing result shows that the sequence obtained by sequencing is completely consistent with the target sequence, and no base mutation, deletion and the like occur.
Example 3: analysis of the relationship between RKHMGR Gene and Carotenoid Synthesis in Rhodosporidium toruloides
1. Agrobacterium mediated transformation of Rhodosporidium toruloides YM25235
The recombinant plasmid pRHRKHMGR is transformed into Rhodosporidium toruloides YM25235 by an agrobacterium-mediated method, transformants are screened by a YPD culture medium containing hygromycin B (hygromycin B) with the final concentration of 150 mug/mL, then genomic DNA of the yeast transformants is extracted according to the steps in the DNA extraction kit specification of Shanghai biological engineering GmbH, and then PCR verification is carried out, wherein the result is shown in FIG. 4.
2. Analysis of changes in the content of carotenoids in RKHMGR gene and Rhodosporidium toruloides
Culturing overexpression strain containing pRHRKHMGR at 28 deg.C for 144h, extracting carotenoid, and determining total carotenoid content (mg/g dry thallus) at 445nm with ultraviolet-visible spectrophotometer using original Rhodosporidium toruloides YM25235 strain as control, as shown in FIG. 5; as can be seen from the graphs, the total carotenoid synthesis of the over-expressed strain YM25235/pRHRKHMGR was significantly increased as compared with the original strain, the carotenoid synthesis of the original control strain was 6.29mg/g, and the carotenoid synthesis of the over-expressed strain YM25235/pRHRKHMGR was 8.06mg/g, i.e., the carotenoid synthesis of the over-expressed strain YM25235/pRHRKHMGR was 1.28 times that of the control strain, indicating that RKHMGR gene could promote the synthesis of total carotenoids, i.e., the RKHMGR nucleic acid sequence was indeed related to the carotenoid synthesis in P.erythraea.
3. Analysis of content variation of components of RKHMGR gene and Rhodosporidium toruloides carotenoids
After culturing the overexpression strain containing pRHRKHMGR at 28 ℃ for 192h, extracting carotenoid, and performing high performance liquid chromatography analysis by taking the original Rhodosporidium toruloides YM25235 strain as a reference, the result is shown in Table 1, and the table shows that the content of carotenoid of the overexpression strain YM25235/pRHRKHMGR, mainly rhodotorubin (torularhodine) and beta-carotene (beta-carotene), is obviously improved compared with the content of the original strain. The contents of rhodotorubin and beta-carotene of the over-expression strain YM25235/pRHRKHMGR are 3.06 times and 1.51 times of those of the control strain respectively. The results indicate that RKHMGR gene is capable of promoting carotenoid synthesis, i.e.RKHMGR nucleic acid sequence is indeed associated with carotenoid synthesis in Rhodosporidium toruloides.
Table 1 shows the change in the content of each carotenoid component in YM25235 and YM25235/RKHMGR strains
Sequence listing
<110> university of Kunming science
<120> 3-hydroxy-3-methylglutaryl coenzyme A reductase gene RKHMGR and application thereof
<160> 4
<170> SIPOSequenceListing 1.0
<210> 1
<211> 3981
<212> DNA
<213> Rhodosporidium toruloides YM25235(Rhodosporidium kratochvilovae YM25235)
<400> 1
atggtcctct cgccgtcggg ctcgtccttc tcgtcgctcc gcgcgcccgt cttccgcctc 60
gcaaggctcg gcaccgcgta cccgatcgag gccattgtca ccgtcttctg cgccgccacc 120
ctcgtctact tccagctcat caaggtcgtt cgcaactcgg acttcctcct ctacgacgac 180
gatgccgtcc taggcgccag cgctgccaac cggcacccgt ccatctcgtt cgccccgtcc 240
accggtctct ggaactcggt ttcgccgtcc gagcccgcac ccgccgatgc cgtcgagctc 300
tggctccggc aggtcatcgt cgaggtgccc aacgcgatgg ctgtcgcccg caagggcgag 360
gtcaaggagc tcctcgacgc caactcagcc ctcgcgtccg ccggcgtcga ggactgccac 420
caggtcggcg gcaagtgctg gacctaccag acccagcccg ccctcaacct caccgtcaag 480
tccttcgcgt tcgactcgct cgtcggtccg acccagttcc tcagcaactt ctcgaccctc 540
ccgctcgaga ccgagacggt cgcgctagag agggtccgcc agggcggccg caagcggtac 600
acgagcttct tcaaccgtgg ccgcgaggac ttcatcgtcg aggacgtcac gcccaacgag 660
gaccgcaagc gcgagatgga gagcgtccag tggatgatct tcgccgccaa ggtctttgtc 720
ctgcgtttct ggggcctcgc caagaaagcc gactcggccg acatcttcgt gatgctcatc 780
gcctacgtcc tcatgcactc gaccttcgtc tcgctctacc tcaacatgaa ccgcctctcg 840
cgctcgctac gccccaactc ggggtcgctc gggttctggc tcgcgaccct ctcgctcgcg 900
tcgtcctgcg tcgcgttcat gttcgccctc ctcacggcgt ggtacctcga gatcaacgtc 960
aacccggtcc tccttggcga ggcgctcccc ttcctcgtca tcacagtcgg gtttgagaag 1020
ccgttcgtcc tcacccgcgc cgtcttctcg cacccggcga tcgggcctgg cggcgcgtac 1080
gcgggtgcgc cgagccgcgg ctcgctcacc ccggtcggca acggcaaggg catgcccatg 1140
aacaacgctt tcggcctgcg cttcgccccg cccgtcccgt cgcgcgacat cgtccgaagc 1200
gccgtcgcca agaccgggta cggcatcatc cgcgactacg cgctcgaggt cgcggtcctc 1260
gtcctcggcg cgatgagcgg cgtcgccggc ctccgcgagt tctgccagct cgcggccgtc 1320
atcctcgtat gggacgcgtt ctgcctcgtc gggttcttcg tctcggtcct caccatcatg 1380
gtcgagatcc accgcatcaa ggttatccgc cacttccgcc gcaccgactc gtccgccgac 1440
ctgtcgcgcc tcctcgacga cccggccgcg ttcgccgacg cggacgcgca agcatcggac 1500
gacgagacgg acgcgccgac cccgtcgctc acgctccgcg agcacttcgt caagctcgtc 1560
acgggcaccg ccccgggcga gaaggcccag tcgggcagcc cgaccgcgcg tctcaaggtc 1620
ctcatcatcg cggcgttcct cacgctgcac tcgctcaacc tcgtcacgac cctcaccgcc 1680
aagacggcgc tcgggcgcca catcgaccac tcgccgtcga cgtcgcacgt cccgcagatc 1740
gacacgtcca accccgtcct gagctcgacg ctccttcagc tcgtcgcatc gcacgaggcc 1800
ggcaccgagc tcctcgtcca cgtcgtcccc gcgctgcact tccgcgccgt cgacctcacg 1860
ctcccgcccg tcgcgccggc gtcgaccgca tcgcaggtca gtgatgcgat gcccaacctc 1920
tcctcggcgt cggcatcggg ctcgttcgcc tcgatcgacc agttcctctc gcgctggacg 1980
cgcctcgtcg gcgaccccat cgtctccaag tggatcgtca tcgcgctcgc catgtcgctc 2040
ttcctcaacg ggtacctgct caagggcatc gcgtccggct ccgactcgtt cgggcacggc 2100
tcggccgccg aggtcgccgc gcgtatcctc ctcgcgagca ccggcgcgga cgccaacaac 2160
gacgacgacg aggcggccaa ggcgcgcctc cgccgctcgt tctcgctcct ccgcgaggag 2220
ctgcagaacg agtggacgca gaaggatgcg gctgtcatgc agcgcgagta cgtcaagcac 2280
gagcgcaagg ccgagcagga ggcgacgcag accatcccga gcgttatcaa gattaccgcc 2340
gcgaagaagg cccggagcgg ctccgcctcg tccgacgact cgtcgccgcc cggctcgccc 2400
atcctcatcc gcacccgctc gtccgcccgc aagaacggcg cgagcctcgc tgccccgtcc 2460
tccagctccg aggcctcgac ggctgtcacg ccgccgcagc gcgagctcaa gctctcgccg 2520
tcgaccgtcg cgctcgtccc catcgagggc attcccgacg ctccgcgcga cctcgacacg 2580
tgcgtcaagc tgttcgacgg cggcaacggt gccgttctcc tcaacgacga ggagatcatc 2640
ctcctggtgc agaagggcaa gatcgccgcg tacgcgctcg agaagctcct caacgaccac 2700
gtccgcgccg tcgcgatccg ccgtgccctc atctcgcgcg cgtcggctcg caagacgctc 2760
gagtcgtccg acctcccgta cctccacttc gactactcgc gcgtgatggg ccagtgctgc 2820
gagaacgtgg taggctacat gcccctgccc gtcggcatcg ccggcccgct ccgcatcgac 2880
ggcgtcgtcc tgcccatccc gatggcgacg accgagggtg cgctcgtcgc gtcgacctcg 2940
cgcggctgca aggcgctcaa cgtctcgggc ggcgtcacga ccgtcgtcgt gcaggacgcg 3000
atgacgcgcg gcccggcgct cacgttcccg agcgtcatca tgtgcgcggc ggcgaagcgc 3060
tgggtcgact cggaggaggg cagcaacatc ctcaaggcgg cgttcaactc gacgtcgcgg 3120
tttgcgcgcc tcaagagcct caaggccgcg atggcgggcc ggacgctcta tgtccggttc 3180
gcgacgcaga cgggcgacgc catgggcatg aacatgatct cgaagggctg cgagcgcgcg 3240
ctcgacgtga tgatgaccga ctacttcccc gagatgacga tcgcgtcgct ctcgggcaac 3300
tactgcaccg acaagaagcc ggcggcgatc aactggatcg agggccgcgg caagagcgtc 3360
gttgccgagg gcatcatccc gggcgaggcg gtcaagtcga tcctcaagac aaccgtggac 3420
gacctcgtca agctcaacgt gaccaagaac ctcatcggtt cggccatggc gggctcgatc 3480
ggcggcaaca acgcgcacgc gagcaacatc ctcactgcca tctacctcgc gacgggccag 3540
gacccggcgc agaacgtcga gtcgagcaac tgcatgacgc tgatggacgc catcaacgac 3600
ggcaaggacc tcctgatcac gtgctcgatg ccgtccatcg aggtcggtac cgtcggcggc 3660
ggcacgatcc tcctcccgca ggcggcgatg ctcgacctcc tcggcgtcaa gggtccgcac 3720
ccgaccgcgc ccggccagaa cgcgcagcag ctcgcgcgca tcgtctgcgc cgccgtcatg 3780
gccggcgagc tctcgctcat gtcggccctc gccgccggct cgctcgtcaa gagccacctc 3840
gcgcacaacc gctcggcgcc tgccacgcct gcgccgcaga cgccgctcat ggcttcgcgg 3900
cctacgacgc ctgcgctcgg tgcgccgcct gcccgcctcg cgccgttgac gaccggcggg 3960
cccgggttcg ccaaagagta a 3981
<210> 1
<211> 1326
<212> PRT
<213> Rhodosporidium toruloides YM25235(Rhodosporidium kratochvilovae YM25235)
<400> 1
Met Val Leu Ser Pro Ser Gly Ser Ser Phe Ser Ser Leu Arg Ala Pro
1 5 10 15
Val Phe Arg Leu Ala Arg Leu Gly Thr Ala Tyr Pro Ile Glu Ala Ile
20 25 30
Val Thr Val Phe Cys Ala Ala Thr Leu Val Tyr Phe Gln Leu Ile Lys
35 40 45
Val Val Arg Asn Ser Asp Phe Leu Leu Tyr Asp Asp Asp Ala Val Leu
50 55 60
Gly Ala Ser Ala Ala Asn Arg His Pro Ser Ile Ser Phe Ala Pro Ser
65 70 75 80
Thr Gly Leu Trp Asn Ser Val Ser Pro Ser Glu Pro Ala Pro Ala Asp
85 90 95
Ala Val Glu Leu Trp Leu Arg Gln Val Ile Val Glu Val Pro Asn Ala
100 105 110
Met Ala Val Ala Arg Lys Gly Glu Val Lys Glu Leu Leu Asp Ala Asn
115 120 125
Ser Ala Leu Ala Ser Ala Gly Val Glu Asp Cys His Gln Val Gly Gly
130 135 140
Lys Cys Trp Thr Tyr Gln Thr Gln Pro Ala Leu Asn Leu Thr Val Lys
145 150 155 160
Ser Phe Ala Phe Asp Ser Leu Val Gly Pro Thr Gln Phe Leu Ser Asn
165 170 175
Phe Ser Thr Leu Pro Leu Glu Thr Glu Thr Val Ala Leu Glu Arg Val
180 185 190
Arg Gln Gly Gly Arg Lys Arg Tyr Thr Ser Phe Phe Asn Arg Gly Arg
195 200 205
Glu Asp Phe Ile Val Glu Asp Val Thr Pro Asn Glu Asp Arg Lys Arg
210 215 220
Glu Met Glu Ser Val Gln Trp Met Ile Phe Ala Ala Lys Val Phe Val
225 230 235 240
Leu Arg Phe Trp Gly Leu Ala Lys Lys Ala Asp Ser Ala Asp Ile Phe
245 250 255
Val Met Leu Ile Ala Tyr Val Leu Met His Ser Thr Phe Val Ser Leu
260 265 270
Tyr Leu Asn Met Asn Arg Leu Ser Arg Ser Leu Arg Pro Asn Ser Gly
275 280 285
Ser Leu Gly Phe Trp Leu Ala Thr Leu Ser Leu Ala Ser Ser Cys Val
290 295 300
Ala Phe Met Phe Ala Leu Leu Thr Ala Trp Tyr Leu Glu Ile Asn Val
305 310 315 320
Asn Pro Val Leu Leu Gly Glu Ala Leu Pro Phe Leu Val Ile Thr Val
325 330 335
Gly Phe Glu Lys Pro Phe Val Leu Thr Arg Ala Val Phe Ser His Pro
340 345 350
Ala Ile Gly Pro Gly Gly Ala Tyr Ala Gly Ala Pro Ser Arg Gly Ser
355 360 365
Leu Thr Pro Val Gly Asn Gly Lys Gly Met Pro Met Asn Asn Ala Phe
370 375 380
Gly Leu Arg Phe Ala Pro Pro Val Pro Ser Arg Asp Ile Val Arg Ser
385 390 395 400
Ala Val Ala Lys Thr Gly Tyr Gly Ile Ile Arg Asp Tyr Ala Leu Glu
405 410 415
Val Ala Val Leu Val Leu Gly Ala Met Ser Gly Val Ala Gly Leu Arg
420 425 430
Glu Phe Cys Gln Leu Ala Ala Val Ile Leu Val Trp Asp Ala Phe Cys
435 440 445
Leu Val Gly Phe Phe Val Ser Val Leu Thr Ile Met Val Glu Ile His
450 455 460
Arg Ile Lys Val Ile Arg His Phe Arg Arg Thr Asp Ser Ser Ala Asp
465 470 475 480
Leu Ser Arg Leu Leu Asp Asp Pro Ala Ala Phe Ala Asp Ala Asp Ala
485 490 495
Gln Ala Ser Asp Asp Glu Thr Asp Ala Pro Thr Pro Ser Leu Thr Leu
500 505 510
Arg Glu His Phe Val Lys Leu Val Thr Gly Thr Ala Pro Gly Glu Lys
515 520 525
Ala Gln Ser Gly Ser Pro Thr Ala Arg Leu Lys Val Leu Ile Ile Ala
530 535 540
Ala Phe Leu Thr Leu His Ser Leu Asn Leu Val Thr Thr Leu Thr Ala
545 550 555 560
Lys Thr Ala Leu Gly Arg His Ile Asp His Ser Pro Ser Thr Ser His
565 570 575
Val Pro Gln Ile Asp Thr Ser Asn Pro Val Leu Ser Ser Thr Leu Leu
580 585 590
Gln Leu Val Ala Ser His Glu Ala Gly Thr Glu Leu Leu Val His Val
595 600 605
Val Pro Ala Leu His Phe Arg Ala Val Asp Leu Thr Leu Pro Pro Val
610 615 620
Ala Pro Ala Ser Thr Ala Ser Gln Val Ser Asp Ala Met Pro Asn Leu
625 630 635 640
Ser Ser Ala Ser Ala Ser Gly Ser Phe Ala Ser Ile Asp Gln Phe Leu
645 650 655
Ser Arg Trp Thr Arg Leu Val Gly Asp Pro Ile Val Ser Lys Trp Ile
660 665 670
Val Ile Ala Leu Ala Met Ser Leu Phe Leu Asn Gly Tyr Leu Leu Lys
675 680 685
Gly Ile Ala Ser Gly Ser Asp Ser Phe Gly His Gly Ser Ala Ala Glu
690 695 700
Val Ala Ala Arg Ile Leu Leu Ala Ser Thr Gly Ala Asp Ala Asn Asn
705 710 715 720
Asp Asp Asp Glu Ala Ala Lys Ala Arg Leu Arg Arg Ser Phe Ser Leu
725 730 735
Leu Arg Glu Glu Leu Gln Asn Glu Trp Thr Gln Lys Asp Ala Ala Val
740 745 750
Met Gln Arg Glu Tyr Val Lys His Glu Arg Lys Ala Glu Gln Glu Ala
755 760 765
Thr Gln Thr Ile Pro Ser Val Ile Lys Ile Thr Ala Ala Lys Lys Ala
770 775 780
Arg Ser Gly Ser Ala Ser Ser Asp Asp Ser Ser Pro Pro Gly Ser Pro
785 790 795 800
Ile Leu Ile Arg Thr Arg Ser Ser Ala Arg Lys Asn Gly Ala Ser Leu
805 810 815
Ala Ala Pro Ser Ser Ser Ser Glu Ala Ser Thr Ala Val Thr Pro Pro
820 825 830
Gln Arg Glu Leu Lys Leu Ser Pro Ser Thr Val Ala Leu Val Pro Ile
835 840 845
Glu Gly Ile Pro Asp Ala Pro Arg Asp Leu Asp Thr Cys Val Lys Leu
850 855 860
Phe Asp Gly Gly Asn Gly Ala Val Leu Leu Asn Asp Glu Glu Ile Ile
865 870 875 880
Leu Leu Val Gln Lys Gly Lys Ile Ala Ala Tyr Ala Leu Glu Lys Leu
885 890 895
Leu Asn Asp His Val Arg Ala Val Ala Ile Arg Arg Ala Leu Ile Ser
900 905 910
Arg Ala Ser Ala Arg Lys Thr Leu Glu Ser Ser Asp Leu Pro Tyr Leu
915 920 925
His Phe Asp Tyr Ser Arg Val Met Gly Gln Cys Cys Glu Asn Val Val
930 935 940
Gly Tyr Met Pro Leu Pro Val Gly Ile Ala Gly Pro Leu Arg Ile Asp
945 950 955 960
Gly Val Val Leu Pro Ile Pro Met Ala Thr Thr Glu Gly Ala Leu Val
965 970 975
Ala Ser Thr Ser Arg Gly Cys Lys Ala Leu Asn Val Ser Gly Gly Val
980 985 990
Thr Thr Val Val Val Gln Asp Ala Met Thr Arg Gly Pro Ala Leu Thr
995 1000 1005
Phe Pro Ser Val Ile Met Cys Ala Ala Ala Lys Arg Trp Val Asp Ser
1010 1015 1020
Glu Glu Gly Ser Asn Ile Leu Lys Ala Ala Phe Asn Ser Thr Ser Arg
1025 1030 1035 1040
Phe Ala Arg Leu Lys Ser Leu Lys Ala Ala Met Ala Gly Arg Thr Leu
1045 1050 1055
Tyr Val Arg Phe Ala Thr Gln Thr Gly Asp Ala Met Gly Met Asn Met
1060 1065 1070
Ile Ser Lys Gly Cys Glu Arg Ala Leu Asp Val Met Met Thr Asp Tyr
1075 1080 1085
Phe Pro Glu Met Thr Ile Ala Ser Leu Ser Gly Asn Tyr Cys Thr Asp
1090 1095 1100
Lys Lys Pro Ala Ala Ile Asn Trp Ile Glu Gly Arg Gly Lys Ser Val
1105 1110 1115 1120
Val Ala Glu Gly Ile Ile Pro Gly Glu Ala Val Lys Ser Ile Leu Lys
1125 1130 1135
Thr Thr Val Asp Asp Leu Val Lys Leu Asn Val Thr Lys Asn Leu Ile
1140 1145 1150
Gly Ser Ala Met Ala Gly Ser Ile Gly Gly Asn Asn Ala His Ala Ser
1155 1160 1165
Asn Ile Leu Thr Ala Ile Tyr Leu Ala Thr Gly Gln Asp Pro Ala Gln
1170 1175 1180
Asn Val Glu Ser Ser Asn Cys Met Thr Leu Met Asp Ala Ile Asn Asp
1185 1190 1195 1200
Gly Lys Asp Leu Leu Ile Thr Cys Ser Met Pro Ser Ile Glu Val Gly
1205 1210 1215
Thr Val Gly Gly Gly Thr Ile Leu Leu Pro Gln Ala Ala Met Leu Asp
1220 1225 1230
Leu Leu Gly Val Lys Gly Pro His Pro Thr Ala Pro Gly Gln Asn Ala
1235 1240 1245
Gln Gln Leu Ala Arg Ile Val Cys Ala Ala Val Met Ala Gly Glu Leu
1250 1255 1260
Ser Leu Met Ser Ala Leu Ala Ala Gly Ser Leu Val Lys Ser His Leu
1265 1270 1275 1280
Ala His Asn Arg Ser Ala Pro Ala Thr Pro Ala Pro Gln Thr Pro Leu
1285 1290 1295
Met Ala Ser Arg Pro Thr Thr Pro Ala Leu Gly Ala Pro Pro Ala Arg
1300 1305 1310
Leu Ala Pro Leu Thr Thr Gly Gly Pro Gly Phe Ala Lys Glu
1315 1320 1325
<210> 3
<211> 28
<212> DNA
<213> Artificial sequence (Artificial)
<400> 3
ttcggatcct tatggtcctc tcgccgtc 28
<210> 4
<211> 28
<212> DNA
<213> Artificial sequence (Artificial)
<400> 4
gcagatatct tactctttgg cgaacccg 28
Claims (4)
1. A3-hydroxy-3-methylglutaryl coenzyme A reductase gene RKHMGR has the nucleotide sequence shown in SEQ ID NO. 1.
2. A recombinant expression vector comprising the 3-hydroxy-3-methylglutaryl-CoA reductase gene, RKHMGR, according to claim 1.
3. A host expression cell containing the 3-hydroxy-3-methylglutaryl-coa reductase gene RKHMGR of claim 1 or the recombinant expression vector of claim 2.
4. 3-hydroxy-3-methylglutaryl coenzyme A reductase gene RKHMGR as claimed in claim 1 in promoting Rhodosporidium toruloides (R)Rhodosporidium kratochvilovae) Use in the production of carotenoids.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911070771.7A CN110747206B (en) | 2019-11-05 | 2019-11-05 | 3-hydroxy-3-methylglutaryl coenzyme A reductase gene RKHMGR and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911070771.7A CN110747206B (en) | 2019-11-05 | 2019-11-05 | 3-hydroxy-3-methylglutaryl coenzyme A reductase gene RKHMGR and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110747206A CN110747206A (en) | 2020-02-04 |
| CN110747206B true CN110747206B (en) | 2021-11-23 |
Family
ID=69282234
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911070771.7A Active CN110747206B (en) | 2019-11-05 | 2019-11-05 | 3-hydroxy-3-methylglutaryl coenzyme A reductase gene RKHMGR and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110747206B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109666683B (en) * | 2019-02-27 | 2021-10-29 | 昆明理工大学 | Acetyl coenzyme A acetyltransferase gene RKAcaT2 and application thereof |
| CN109777815B (en) * | 2019-03-28 | 2021-10-29 | 昆明理工大学 | HMG-CoA synthetase gene RKHMGCS and application thereof |
| CN112410355B (en) * | 2020-11-23 | 2022-03-25 | 昆明理工大学 | Acyl-coenzyme A oxidase 2 gene RKACOX2 and application thereof |
| CN113621630B (en) * | 2021-08-05 | 2023-03-24 | 昆明理工大学 | 3-ketoacyl-CoA thiolase gene RkACAA1-1 and application thereof |
| CN113621631A (en) * | 2021-08-11 | 2021-11-09 | 昆明理工大学 | Mevalonate kinase gene RKMK and application thereof |
| CN114540356B (en) * | 2022-02-25 | 2023-09-01 | 昆明理工大学 | Rhodosporidium toruloides promoter and application thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1338937A (en) * | 1999-02-06 | 2002-03-06 | 阿斯特拉曾尼卡有限公司 | Use of 3-hydroxy-3-methylglutaryl coenzym a reductase inhibitors for the manufacture of a medicament for the treatment of diabetic neuropathy |
| CN1436192A (en) * | 2000-06-15 | 2003-08-13 | 布里斯托尔-迈尔斯斯奎布公司 | HMG-CoA reductase inhibitors and method |
| CN109666683A (en) * | 2019-02-27 | 2019-04-23 | 昆明理工大学 | Acetyl coenzyme A acetyl transferase gene RKAcaT2 and its application |
| CN109777815A (en) * | 2019-03-28 | 2019-05-21 | 昆明理工大学 | HMG-CoA synthase gene RKHMGCS and its application |
| CN111454854A (en) * | 2020-05-02 | 2020-07-28 | 昆明理工大学 | Rhodosporidium toruloides gene engineering strain for producing astaxanthin |
| CN112410355A (en) * | 2020-11-23 | 2021-02-26 | 昆明理工大学 | Acyl-coenzyme A oxidase 2 gene RKACOX2 and application thereof |
| CN113430215A (en) * | 2021-06-03 | 2021-09-24 | 昆明理工大学 | Acetyl CoA synthetase gene RKACS1 and application thereof |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103589650A (en) * | 2005-03-18 | 2014-02-19 | 米克罗比亚公司 | Production of carotenoids in oleaginous yeast and fungi |
| US8691555B2 (en) * | 2006-09-28 | 2014-04-08 | Dsm Ip Assests B.V. | Production of carotenoids in oleaginous yeast and fungi |
| MX2010011068A (en) * | 2008-04-08 | 2010-11-04 | Amyris Biotechnologies Inc | Expression of heterologous sequences. |
| US20120149886A1 (en) * | 2008-04-10 | 2012-06-14 | Microbia, Inc. | Production of carotenoids in oleaginous yeast and fungi |
| CN101748170A (en) * | 2010-01-22 | 2010-06-23 | 西北大学 | Method for preparing 1-deoxy-D-xylulose with chemical method-enzymatic method |
| CN102787074B (en) * | 2011-05-20 | 2016-06-08 | 罗盖特兄弟公司 | Novel microalgae strain for producing squalene |
| US9834800B2 (en) * | 2012-08-17 | 2017-12-05 | Evolva Sa | Increased production of terpenes and terpenoids |
| CN102911882B (en) * | 2012-10-25 | 2014-02-12 | 南京师范大学 | Rhodosporidium kratochvilovae and application of same in preparation of carotenoid and single-cell protein |
| CN103484520B (en) * | 2013-09-30 | 2016-05-04 | 上海化工研究院 | A kind of fermentation accelerant strengthening carrotene zymotechnique that adopts |
| US20150167041A1 (en) * | 2013-12-14 | 2015-06-18 | E I Du Pont De Nemours And Company | Astaxanthin production using a recombinant microbial host cell |
| CN104762242B (en) * | 2014-01-02 | 2018-10-26 | 清华大学 | The production bacterium of mevalonic acid and the method for producing mevalonic acid |
| US10131922B2 (en) * | 2015-03-10 | 2018-11-20 | Indian Institute Of Science Education And Research | Increase production of isoprenoids in saccharomyces cerevisiae by carotenoid optimization and screening |
| CN107636155B (en) * | 2015-04-03 | 2022-01-07 | 淡马锡生命科学研究院有限公司 | D-amino acid inducible gene expression system of rhodosporidium and rhodotorula |
| EP3426791A1 (en) * | 2016-03-11 | 2019-01-16 | Evolva SA | Production of steviol glycosides in recombinant hosts |
| US11149291B2 (en) * | 2017-07-12 | 2021-10-19 | Biomedican, Inc. | Production of cannabinoids in yeast |
| CN109536518A (en) * | 2018-10-31 | 2019-03-29 | 昆明理工大学 | A kind of Phytoene dehydrogenase gene RKcrtI and its application |
-
2019
- 2019-11-05 CN CN201911070771.7A patent/CN110747206B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1338937A (en) * | 1999-02-06 | 2002-03-06 | 阿斯特拉曾尼卡有限公司 | Use of 3-hydroxy-3-methylglutaryl coenzym a reductase inhibitors for the manufacture of a medicament for the treatment of diabetic neuropathy |
| CN1436192A (en) * | 2000-06-15 | 2003-08-13 | 布里斯托尔-迈尔斯斯奎布公司 | HMG-CoA reductase inhibitors and method |
| CN109666683A (en) * | 2019-02-27 | 2019-04-23 | 昆明理工大学 | Acetyl coenzyme A acetyl transferase gene RKAcaT2 and its application |
| CN109777815A (en) * | 2019-03-28 | 2019-05-21 | 昆明理工大学 | HMG-CoA synthase gene RKHMGCS and its application |
| CN111454854A (en) * | 2020-05-02 | 2020-07-28 | 昆明理工大学 | Rhodosporidium toruloides gene engineering strain for producing astaxanthin |
| CN112410355A (en) * | 2020-11-23 | 2021-02-26 | 昆明理工大学 | Acyl-coenzyme A oxidase 2 gene RKACOX2 and application thereof |
| CN113430215A (en) * | 2021-06-03 | 2021-09-24 | 昆明理工大学 | Acetyl CoA synthetase gene RKACS1 and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| "Engineering of the carotenoid pathway in Xanthophyllomyces dendrorhous leading to the synthesis of zeaxanthin";Hendrik Pollmann等;《Appl Microbiol Biotechnol》;20160816;第103-111页 * |
| "Rhodotorula toruloides NP11 hydroxymethylglutaryl-CoA reductase (NADPH) partial mRNA";Zhu,Z.等;《Genbank Database》;20160429;Accession No：XM_016417713.1 * |
| "过表达RkHMGCR 基因对红冬孢酵母类胡萝卜素合成的影响";和美霞等;《云南大学学报（ 自然科学版）》;20210910;第43卷(第5期);第1018-1027页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110747206A (en) | 2020-02-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110747206B (en) | 3-hydroxy-3-methylglutaryl coenzyme A reductase gene RKHMGR and application thereof | |
| CN109666683B (en) | Acetyl coenzyme A acetyltransferase gene RKAcaT2 and application thereof | |
| CN109777815B (en) | HMG-CoA synthetase gene RKHMGCS and application thereof | |
| Kildegaard et al. | Engineering of Yarrowia lipolytica for production of astaxanthin | |
| CN112410355B (en) | Acyl-coenzyme A oxidase 2 gene RKACOX2 and application thereof | |
| KR102202606B1 (en) | A microorganism for producing a bio-retinol and a method for preparing a bio-retinol using the same | |
| CN110106209B (en) | Method for positioning and synthesizing terpenoid by using yarrowia lipolytica pathway | |
| CN111454854B (en) | Rhodosporidium toruloides gene engineering strain for producing astaxanthin | |
| CN109536518A (en) | A kind of Phytoene dehydrogenase gene RKcrtI and its application | |
| CN113430215B (en) | Acetyl CoA synthetase gene RKACS1 and application thereof | |
| CN115011616B (en) | Acetaldehyde dehydrogenase gene RKALDH and application thereof | |
| US10308691B2 (en) | Methods for tuning carotenoid production levels and compositions in rhodosporidium and rhodotorula genera | |
| CN106754979B (en) | Gene for regulating and controlling long-chain fatty acid transport of candida tropicalis and application of gene | |
| CN108624600B (en) | Application of zinc finger transcription factor gene RkMsn4 | |
| CN116286880B (en) | Peroxisome increment factor gene RkPEX and application thereof | |
| CN112608936A (en) | Promoter for regulating and controlling expression of yeast exogenous gene, regulation and control method and application thereof | |
| CN115725560B (en) | Pinus massoniana multifunctional terpene synthase mutant and application thereof in production of sesquiterpene products | |
| CN117701548A (en) | Sandalene synthetase mutant and application thereof | |
| CN114525215B (en) | Recombinant strain for producing terpenoid, construction method thereof, method for producing terpenoid through fermentation and application of recombinant strain | |
| CN114989274B (en) | Phaeodactylum tricornutum Myb transcription factor PtMYB3 gene and encoding protein and application thereof | |
| CN109609519A (en) | A kind of gene RKcrtYB and its application | |
| CN117229934A (en) | Genetically engineered bacterium for synthesizing carotenoid, construction method and application thereof | |
| JP2011125272A (en) | New sesquiterpene synthetase gene and method for producing sesquiterpene by utilizing the same | |
| Huang et al. | GATA transcription factor WC2 regulates the biosynthesis of astaxanthin in yeast Xanthophyllomyces dendrorhous | |
| CN112029782B (en) | Beta-carotene hydroxylase, gene and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |