CN110613128A - Low-temperature extraction production process of active sheep placenta - Google Patents
Low-temperature extraction production process of active sheep placenta Download PDFInfo
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- 241001494479 Pecora Species 0.000 title claims abstract description 100
- 238000000605 extraction Methods 0.000 title claims abstract description 24
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 20
- 238000007710 freezing Methods 0.000 claims abstract description 40
- 230000008014 freezing Effects 0.000 claims abstract description 40
- 239000006228 supernatant Substances 0.000 claims abstract description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 21
- 108090000526 Papain Proteins 0.000 claims abstract description 20
- 239000004365 Protease Substances 0.000 claims abstract description 20
- 102000004142 Trypsin Human genes 0.000 claims abstract description 20
- 108090000631 Trypsin Proteins 0.000 claims abstract description 20
- 230000007935 neutral effect Effects 0.000 claims abstract description 20
- 229940055729 papain Drugs 0.000 claims abstract description 20
- 235000019834 papain Nutrition 0.000 claims abstract description 20
- 239000012588 trypsin Substances 0.000 claims abstract description 20
- 238000003756 stirring Methods 0.000 claims abstract description 18
- 238000000034 method Methods 0.000 claims abstract description 17
- 238000002347 injection Methods 0.000 claims abstract description 16
- 239000007924 injection Substances 0.000 claims abstract description 16
- 239000012535 impurity Substances 0.000 claims abstract description 15
- 230000008569 process Effects 0.000 claims abstract description 15
- 239000002002 slurry Substances 0.000 claims abstract description 15
- 238000004140 cleaning Methods 0.000 claims abstract description 11
- 238000002156 mixing Methods 0.000 claims abstract description 8
- 230000001376 precipitating effect Effects 0.000 claims abstract description 8
- 238000001556 precipitation Methods 0.000 claims abstract description 8
- 238000002791 soaking Methods 0.000 claims abstract description 8
- 238000011049 filling Methods 0.000 claims abstract description 7
- 238000004108 freeze drying Methods 0.000 claims abstract description 7
- 239000000203 mixture Substances 0.000 claims abstract description 6
- 238000004537 pulping Methods 0.000 claims abstract description 6
- 230000015556 catabolic process Effects 0.000 claims abstract description 5
- 238000005520 cutting process Methods 0.000 claims abstract description 5
- 238000006731 degradation reaction Methods 0.000 claims abstract description 5
- 238000000227 grinding Methods 0.000 claims abstract description 5
- 238000001816 cooling Methods 0.000 claims abstract description 4
- 238000001914 filtration Methods 0.000 claims abstract description 4
- 239000000843 powder Substances 0.000 claims abstract description 4
- 239000007788 liquid Substances 0.000 claims description 11
- 238000003860 storage Methods 0.000 claims description 9
- 230000000593 degrading effect Effects 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 4
- 210000002966 serum Anatomy 0.000 claims description 4
- 229910001220 stainless steel Inorganic materials 0.000 claims description 4
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- 102000004190 Enzymes Human genes 0.000 description 9
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- 229940088598 enzyme Drugs 0.000 description 9
- 150000001413 amino acids Chemical class 0.000 description 4
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- CFWRDBDJAOHXSH-SECBINFHSA-N 2-azaniumylethyl [(2r)-2,3-diacetyloxypropyl] phosphate Chemical compound CC(=O)OC[C@@H](OC(C)=O)COP(O)(=O)OCCN CFWRDBDJAOHXSH-SECBINFHSA-N 0.000 description 1
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- 238000009776 industrial production Methods 0.000 description 1
- 229940047124 interferons Drugs 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 229940067606 lecithin Drugs 0.000 description 1
- 235000010445 lecithin Nutrition 0.000 description 1
- 239000000787 lecithin Substances 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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Abstract
The invention provides a low-temperature extraction production process of active sheep placenta extract, which comprises the following steps of S100, soaking, unfreezing, impurity removing and cleaning sheep placenta; s200, cutting, crushing and grinding the sheep placenta; s300, pulping, and then putting into a freezer for quick cooling; s400, freezing, taking out, putting into a first enzymolysis tank, adding neutral trypsin and papain, stirring, and precipitating; s500, putting the raw slurry obtained in the step S300 into a first enzymolysis tank, and performing secondary enzymolysis and precipitation; s600, mixing the supernatant obtained in the step S500 with the supernatant obtained in the step S400, and freezing the mixture; s700, putting the frozen supernatant into a second enzymolysis tank, adding injection water for degradation and layering, putting the supernatant into a centrifuge, and filtering to obtain primary sheep placenta; s800, freezing the primary sheep placenta again, adding water for jetting, and precipitating again to obtain high-purity sheep placenta; s900, freeze-drying by a freeze dryer to obtain powder, and filling, wherein the whole process is simple to operate, low in cost and high in extraction efficiency.
Description
Technical Field
The invention relates to the technical field of sheep placenta extract extraction, in particular to a low-temperature extraction production process of active sheep placenta extract.
Background
Sheep placenta extract is a common health product and is a general name of small molecular compounds with immunocompetence extracted from placenta, and the sheep placenta extract contains: immunoglobulin and anti-aging factor, which are enzymes and interferons with high application value, and sheep placenta contains rich protein, amino acids, lecithin, cephalin, multiple vitamins, trace elements and the like. The theory of traditional Chinese medicine holds that the placenta is dry and salty, and enters lung and kidney meridians. Has the functions of benefiting qi, nourishing blood, benefiting essence, nourishing, beautifying and strengthening. The efficacy mainly comprises the following points: has health protecting effect on skin, and has effects in improving immunity and motion ability, relieving fatigue, resisting aging, and improving cell immunity. Because sheep placenta has many efficacies, the extraction and separation of the effective components thereof by modern biotechnology and modern chemical method become important subjects.
In the prior art, the production process for extracting the sheep placenta extract is generally complex, the using amount of enzyme in the extraction process is large, and the extraction efficiency of polypeptide and amino acid cannot be increased on the basis of keeping natural active substances in the sheep placenta in the selection of the enzyme in the prior art, so that the extraction efficiency of the sheep placenta extract in the prior art is very low, the utilization efficiency of the sheep placenta extract is not high, and great resource waste is caused.
Disclosure of Invention
The invention mainly aims to provide a low-temperature extraction production process of active sheep placenta, which overcomes the defects of the prior art, has simple and easy operation method and high production efficiency, and can effectively solve the problem that the prior art can use a large amount of enzyme, thereby ensuring that the production cost is low and the process is suitable for industrial production.
The invention also aims to provide a low-temperature extraction production process of the active sheep placenta, which can increase the extraction efficiency of polypeptide and amino acid in the sheep placenta on the basis of keeping natural active substances in the sheep placenta, thereby effectively improving the utilization efficiency of the sheep placenta and having remarkable economic and social benefits.
In order to achieve the above purposes, the technical scheme adopted by the invention is as follows: a low-temperature extraction production process of active sheep placenta extract comprises the following steps:
s100, pretreatment of sheep placenta: soaking, unfreezing, impurity removing and cleaning sheep placenta;
s200, cutting into blocks and grinding into slurry: cutting the dried sheep placenta into blocks, putting the blocks and injection water into a tissue crusher to be crushed into pasty dilute tire mud, and immediately putting the dilute tire mud into a low-temperature pulping machine to be ground into pulp;
s300, first freezing and sublimating: filling the slurried sheep placenta into a first movable type slurry storage tank, then putting the sheep placenta into a freezing warehouse with the freezing temperature of-20 ℃ for quick cooling, and moving out of the freezing warehouse after 3 hours;
s400, first enzymolysis: transferring the liquid which is removed from the freezer and is melted into a flowable liquid into a first enzymolysis tank, adding neutral trypsin and papain into the first enzymolysis tank, stirring the neutral trypsin and the papain simultaneously, naturally precipitating for 3 hours after stirring is finished, and then conveying supernatant liquid of the neutral trypsin and papain to a second movable slurry storage tank;
s500, second enzymolysis: putting the raw slurry obtained in the step S300 into a first enzymolysis tank, adding 1.1% of neutral trypsin and papain into the first enzymolysis tank, stirring and mixing the neutral trypsin and the papain simultaneously, and naturally precipitating the mixture for 3 hours after stirring to obtain a supernatant;
s600, mixed freezing of supernatant: mixing the supernatant in the step S500 with the supernatant in the second movable slurry storage tank in the step S400, and then putting the mixture into a freezer for freezing;
s700 degradation, layering and centrifugation: putting the frozen supernatant into a second enzymolysis tank, adding 20% of injection water for degrading and layering again, taking the layered supernatant, and putting the layered supernatant into a centrifugal machine for filtering to obtain primary sheep placenta;
s800, second freezing sublimation: freezing the primary sheep placenta extract in a freezer, adding injection water for secondary precipitation after freezing, and obtaining high-purity sheep placenta extract;
s900, freeze-drying and packaging: and (3) freeze-drying the high-purity sheep placenta extract obtained in the step (S800) by a freeze dryer to form sheep placenta extract powder, and filling.
According to an embodiment of the present invention, the step S100 includes the steps of:
s110, taking out the sheep placenta from the freezer, and then immediately putting the sheep placenta into a stainless steel soaking pool to be soaked and unfrozen by injection water cooled to normal temperature;
s120, placing the unfrozen sheep placenta in a first operation table for moisture control and drying, and then manually removing impurities such as grease on the sheep placenta;
s130, putting the sheep placenta after impurity removal into a cleaning tank filled with water injection for cleaning, and putting the cleaned sheep placenta on a second operation table for drying.
Preferably, the size of the cut pieces in the step S200 is 6-8 cm, and the amount of the injected water accounts for 30% of the total amount.
Preferably, the total amount of the neutral trypsin and the papain put in step S400 is 1.7% of the total amount of the pulp.
Preferably, the stirring time of step S400 and the stirring time of step S500 are both 37 min.
Preferably, the freezing temperature in the freezing process in the steps S600 and S700 is-12 ℃ and the freezing time is 2.5 hours.
Preferably, the time for degradation and delamination in step S700 is 2 hours.
Preferably, the amount of the water for injection added in step S800 is 20% of the total amount, and the precipitation time is 2 hours.
The invention has the beneficial effects that: (1) the requirement on the dosage of the enzyme can be effectively reduced, so that the production cost is reduced; (2) by means of stirring, the whole enzymolysis process can be effectively accelerated, and meanwhile, the enzyme and the sheep placenta can be fully contacted, so that the whole enzymolysis is more sufficient, and the extraction efficiency is improved; (3) by pretreating the sheep placenta, impurities contained in the sheep placenta can be effectively removed, so that the purity of the produced sheep placenta is improved; (4) through freezing treatment many times, can remove colour and taste in the sheep placenta effectively, make the sheep placenta of producing colorless tasteless, the quality is higher from this.
Detailed Description
The following description is presented to disclose the invention so as to enable any person skilled in the art to practice the invention. The preferred embodiments in the following description are given by way of example only, and other obvious variations will occur to those skilled in the art.
A low-temperature extraction production process of active sheep placenta extract comprises the following steps:
(1) thawing, namely removing the sheep placenta from the freezer, putting the sheep placenta into a stainless steel soaking pool, and soaking and thawing the sheep placenta by using injection water cooled to normal temperature, wherein the purpose of soaking is to diffuse impurities adsorbed on the sheep placenta so as to facilitate subsequent manual impurity removal;
(2) remove the impurity, treat the sheep placenta in the stainless steel soaks the pond and unfreeze the back, drag for it immediately to the accuse moisture on the first operation panel, be favorable to follow-up contact between enzyme and the sheep placenta with its moisture accuse futilely to improve its enzymolysis process, reject debris such as grease through the manual work, can effectively reject totally with the impurity on the sheep placenta fast, thereby improved the purity of the sheep placenta of extracting.
(3) Cleaning, namely putting the sheep placenta after impurity removal into a cleaning tank, adding injection water to clean the sheep placenta, fishing out after cleaning, putting on a second operation table for draining, and cleaning the impurities remained in the impurity removal process in the process to obtain clean sheep placenta.
(4) The stripping and slicing is to control the dried sheep placenta to be cut into the square size of 6-8 cm, so that the sheep placenta can be more quickly crushed in the tissue crusher, the whole crushing process is quicker, and the working efficiency is effectively improved.
(5) Crushing, namely putting the cut sheep placenta into a tissue crusher, adding 30% of injection water to crush the sheep placenta, and crushing the sheep placenta into meat paste-shaped dilute sheep placenta mud, so that preparation can be made for a subsequent pulping process.
(6) Grinding, and feeding the crushed placenta caprae seu ovis into a low-temperature pulping machine for pulping.
(7) And (3) performing first freezing sublimation, filling the slurried sheep placenta into a first movable slurry storage tank, then quickly cooling the slurry in a freezing warehouse with the freezing temperature of-20 ℃, and moving out of the freezing warehouse after 3 hours.
(8) Carrying out first enzymolysis, namely transferring the liquid which is removed from the freezer and melted into flowable liquid into a first enzymolysis tank, adding neutral trypsin and papain into the first enzymolysis tank, controlling the adding amount of the enzymes to be 1.7 percent of the total amount of the slurry, simultaneously stirring the liquid for 37min, and stirring the liquid in the enzymolysis process to be beneficial to the neutral trypsin and the papain to be fully contacted with the sheep placenta after grinding respectively, thereby greatly improving the enzymolysis efficiency of the whole enzymolysis process, effectively reducing the using amount of the enzymes, saving the production cost and improving the extraction rate, in addition, the enzymolysis process only adopts the neutral trypsin and the papain to carry out double enzymolysis on the liquid, thereby effectively simplifying the whole extraction process, naturally precipitating for 3 hours after the stirring is finished, transferring the supernatant in the first enzymolysis tank into a second movable slurry storage tank, meanwhile, the sediment in the first enzymolysis tank is left in the tube.
(9) Performing second enzymolysis, namely putting the raw serum obtained in the step S300 into a first enzymolysis tank, adding 1.1% of neutral trypsin and papain into the first enzymolysis tank, and stirring and mixing the neutral trypsin and the papain simultaneously, so that the neutral trypsin and the papain can perform double enzymolysis precipitation on the raw serum obtained in the step S300, the double hydrolysis precipitation of the neutral trypsin and the papain can retain active substances in the sheep placenta and increase the content of polypeptides and amino acids generated by enzymolysis of the sheep placenta, and naturally precipitating the sheep placenta for 3 hours after stirring to obtain supernatant;
(10) and (3) mixing and freezing the supernatant, mixing the supernatant in the step S500 with the supernatant in the second movable serum storage tank in the step S400, and then putting the mixture into a freezing warehouse for freezing at the freezing temperature of-12 ℃ for 2.5 hours, wherein the smell and color of the prepared primary sheep placenta can be treated in the freezing process, so that the subsequently produced sheep placenta is colorless and tasteless, and the quality is higher.
(11) And (3) degrading, layering and centrifuging, putting the frozen supernatant into a second enzymolysis tank, adding 20% of injection water for degrading and layering again for 2 hours, taking the layered supernatant, and putting the layered supernatant into a centrifugal machine for filtering to obtain the primary sheep placenta.
(12) Performing secondary freezing sublimation, namely putting the primary sheep placenta extract into a freezing chamber again for freezing at the temperature of-12 ℃ for 2 hours, and adding injection water for secondary precipitation after freezing is finished to obtain high-purity sheep placenta extract;
(13) freeze-drying and packaging, and finally freeze-drying the high-purity sheep placenta extract by a freeze dryer to form sheep placenta extract powder, and then filling.
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are merely illustrative of the principles of the invention, but that various changes and modifications may be made without departing from the spirit and scope of the invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (8)
1. A low-temperature extraction production process of active sheep placenta extract is characterized by comprising the following production steps:
s100, pretreatment of sheep placenta: soaking, unfreezing, impurity removing and cleaning sheep placenta;
s200, cutting into blocks and grinding into slurry: cutting the dried sheep placenta into blocks, putting the blocks and injection water into a tissue crusher to be crushed into pasty dilute tire mud, and immediately putting the dilute tire mud into a low-temperature pulping machine to be ground into pulp;
s300, first freezing and sublimating: filling the slurried sheep placenta into a first movable type slurry storage tank, then putting the sheep placenta into a freezing warehouse with the freezing temperature of-20 ℃ for quick cooling, and moving out of the freezing warehouse after 3 hours;
s400, first enzymolysis: transferring the liquid which is removed from the freezer and is melted into a flowable liquid into a first enzymolysis tank, adding neutral trypsin and papain into the first enzymolysis tank, stirring the neutral trypsin and the papain simultaneously, naturally precipitating for 3 hours after stirring is finished, and then conveying supernatant liquid of the neutral trypsin and papain to a second movable slurry storage tank;
s500, second enzymolysis: putting the raw slurry obtained in the step S300 into a first enzymolysis tank, adding 1.1% of neutral trypsin and papain into the first enzymolysis tank, stirring and mixing the neutral trypsin and the papain simultaneously, and naturally precipitating the mixture for 3 hours after stirring to obtain a supernatant;
s600, mixed freezing of supernatant: mixing the supernatant in the step S500 with the supernatant in the second movable slurry storage tank in the step S400, and then putting the mixture into a freezer for freezing;
s700 degradation, layering and centrifugation: putting the frozen supernatant into a second enzymolysis tank, adding 20% of injection water for degrading and layering again, taking the layered supernatant, and putting the layered supernatant into a centrifugal machine for filtering to obtain primary sheep placenta;
s800, second freezing sublimation: freezing the primary sheep placenta extract in a freezer, adding injection water for secondary precipitation after freezing, and obtaining high-purity sheep placenta extract;
s900, freeze-drying and packaging: and (3) freeze-drying the high-purity sheep placenta extract obtained in the step (S800) by a freeze dryer to form sheep placenta extract powder, and filling.
2. The low-temperature extraction production process of active sheep placenta extract as claimed in claim 1, wherein the step S100 comprises the steps of:
s110, taking out the sheep placenta from the freezer, and then immediately putting the sheep placenta into a stainless steel soaking pool to be soaked and unfrozen by injection water cooled to normal temperature;
s120, placing the unfrozen sheep placenta in a first operation table for moisture control and drying, and then manually removing impurities such as grease on the sheep placenta;
s130, putting the sheep placenta after impurity removal into a cleaning tank filled with water injection for cleaning, and putting the cleaned sheep placenta on a second operation table for drying.
3. The low-temperature extraction production process of active sheep placenta extract as claimed in claim 2, wherein the size of the cut pieces in step S200 is 6-8 cm, and the amount of injected water accounts for 30% of the total amount.
4. The low-temperature extraction production process of active sheep placenta extract as claimed in claim 3, wherein the total amount of neutral trypsin and papain added in step S400 is 1.7% of the total amount of the serum.
5. The low-temperature extraction production process of active sheep placenta extract as claimed in claim 4, wherein the stirring time of step S400 and step S500 is 37 min.
6. The process for extracting active sheep placenta extract at low temperature according to claim 5, wherein the freezing temperature in the step S600 and the freezing time in the step S700 are both-12 ℃ and 2.5 hours.
7. The low-temperature extraction production process of active sheep placenta extract as claimed in claim 6, wherein the time for degradation and stratification in step S700 is 2 hours.
8. The process for producing active sheep placenta extract at low temperature as claimed in claim 7, wherein the amount of water for injection added in step S800 is 20% of the total amount, and the precipitation time is 2 hours.
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| CN2019106721815 | 2019-07-24 | ||
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111297786A (en) * | 2020-03-20 | 2020-06-19 | 扬州扬大联环药业基因工程有限公司 | Method for extracting active small molecules from sheep embryos in large scale |
| CN114592024A (en) * | 2022-04-01 | 2022-06-07 | 珠海市华喜生物科技有限公司 | Sheep placenta polypeptide and preparation method and application thereof |
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Application publication date: 20191227 |