CN110554651B - Private Internet of things system for measuring and controlling temperature of microfluidic chip - Google Patents
Private Internet of things system for measuring and controlling temperature of microfluidic chip Download PDFInfo
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- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
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- G01K—MEASURING TEMPERATURE; MEASURING QUANTITY OF HEAT; THERMALLY-SENSITIVE ELEMENTS NOT OTHERWISE PROVIDED FOR
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- G05D—SYSTEMS FOR CONTROLLING OR REGULATING NON-ELECTRIC VARIABLES
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Abstract
The invention provides a private Internet of things system for measuring and controlling the temperature of a microfluidic chip based on a B/S mode, which has high intelligent automation degree and belongs to the field of Internet of things and microfluidics. The invention comprises the following steps: the Web client is connected with the temperature field measurement and control unit through the Internet of things network transmission system and used for inputting a temperature measurement instruction and a temperature control instruction and sending the temperature measurement instruction to the temperature field measurement and control unit; the temperature field measurement and control unit is used for measuring the temperature or the temperature field of the specified position of the microfluidic chip through the temperature sensor according to the received temperature measurement instruction, and controlling the temperature of the microfluidic chip by using the temperature control device according to the received temperature control instruction; a Web server is embedded in the temperature field measurement and control unit; and the internet of things network transmission system is used for establishing and ensuring the network communication connection between the Web client and the temperature field measurement and control unit 3 through the Web server and the wide area network.
Description
Technical Field
The invention relates to a private Internet of things system, in particular to a private Internet of things system for micro-fluidic chip temperature field measurement and temperature control based on a B/S mode, and belongs to the field of Internet of things and micro-fluidic.
Background
The internet of things is used for connecting various information sensing devices and intelligent execution devices with the internet so as to realize interconnection and intercommunication of people, machines and things at any time and any place. Accessing various devices to the internet is an implementation means of the internet of things, and the purpose of the internet of things is to better implement services for individuals, enterprises, organizations, governments and the like by providing information or information interaction. The technology of the internet of things has played a very great role in the application of smart cities, smart agriculture, smart manufacturing, smart power grids and the like through the rapid development in recent years. The existing internet of things system is developed from the perspective of a technical developer. For example, a large-scale internet of things cloud server is used to connect with internet of things equipment and a user control terminal. However, the internet of things system developed from the perspective of the technology developer mainly exists: (1) the potential risks of leakage, monitoring and misuse of the private information of the user. For example, an internet of things platform manager has the ability to see the working conditions of the intelligent equipment in the home of the user, and further can analyze the living habits of the user; if the intelligent sound box or the intelligent camera exists, real-time monitoring can be achieved. Although there are relevant legal or ethical constraints, the above risks are always present. (2) The system provides a public platform to access websites or application programs, and is easy to attract lawbreakers to carry out various network security attacks on the users because the system stores a large amount of user information and information of the internet of things system of the users. (3) The user wants to expand the function of the internet of things, and the development difficulty is high. For common internet of things users, especially personal users, enterprise users, organization users, and the like, the satisfaction degree and the safety degree of the service provided by the internet of things system are often more concerned. The ultra-large capacity and large data of the internet of things service provider are not required for users. Because the number of the internet of things devices which are generally required to be used is not large, whether the internet of things system can provide services meeting the requirements of the internet of things system and whether the safety of the system and the safety of information generated by the system can be completely guaranteed is more concerned. Therefore, for the users of the common internet of things, the users really need the private internet of things system.
Meanwhile, the internet of things technology is only a tool technology, various devices are connected to the internet to form the internet of things, and the value of the internet of things can be fully exerted only by providing services for solving various problems in combination with specific application occasions. At present, the microfluidic technology has important research and application values in the fields of medicine, biology, chemistry and the like, and is considered to have great development potential and application prospect in the fields. Temperature is an extremely important parameter or variable in most research and applications in the medical, biological, chemical fields. With the combination of the microfluidic technology and the above fields, how to accurately and precisely measure the temperature in the microfluidic chip and accurately and precisely control the temperature of the chip in the related research and application becomes one of the research hotspots and difficulties in the microfluidic field. And accurate and precise temperature measurement is a precondition for accurately and precisely controlling the temperature of the chip. At present, the temperature measurement methods in the microfluidic field mainly include microelectrode temperature sensor temperature measurement, temperature measurement with temperature-sensitive fluorescent indicator, infrared radiation temperature measurement, thermochromic liquid crystal temperature measurement, and the like. The microelectrode temperature sensor and the infrared radiation thermometer are used for measuring local temperature and cannot obtain spatial distribution information of the temperature. Although temperature sensing fluorescent indicator temperature measurement, infrared radiation imaging temperature measurement and thermochromic liquid crystal temperature measurement can obtain spatial distribution information of temperature, the temperature measurement methods can only obtain plane temperature field information, and if measurement results need to be converted into electric signals or digital signals, compared with methods of microelectrode temperature sensor temperature measurement, the temperature measurement methods are easy to introduce more external interference and errors. In addition, in most of research and application related to the fields of medicine, biology, chemistry and the like which use temperature as an important parameter or variable, the characteristics of long experimental period, many experimental times and the like exist. At present, most methods are to set in advance in equipment, but not to set remote parameters, and after the setting is finished, the equipment needs to wait for long-time operation, and the state of a chip cannot be monitored in real time or cannot be monitored and alarmed remotely and in real time in the process. Because degree of automation, intellectuality is low at whole in-process, lead to that entire system's efficiency is not high, time and manpower are extravagant serious, the easy misoperation that appears, the system breaks down and can not in time report to the police scheduling problem. The development of the technology of the internet of things provides opportunities for solving the problems.
Disclosure of Invention
Aiming at the problems, the invention provides a private Internet of things system for micro-fluidic chip temperature measurement and control based on a B/S mode, which has high intelligent automation degree.
The invention relates to a private Internet of things system for measuring and controlling the temperature of a microfluidic chip, which comprises:
the Web client 11 is connected with the temperature field measurement and control unit 3 through the Internet of things network transmission system 10 and is used for inputting a temperature measurement instruction and a temperature control instruction and sending the temperature measurement instruction to the temperature field measurement and control unit 3;
the temperature field measurement and control unit 3 is used for measuring the temperature or the temperature field of the specified position of the microfluidic chip 5 through the temperature sensor according to the received temperature measurement instruction, and controlling the temperature of the microfluidic chip 5 through the temperature control device 4 according to the received temperature control instruction; a Web server is embedded in the temperature field measurement and control unit 3;
and the internet of things network transmission system 10 is used for establishing and ensuring the network communication connection between the Web client 11 and the temperature field measurement and control unit 3 through the Web server and the wide area network 1.
Preferably, the Web server is configured to wait for network connection and security authentication of the Web client 11, and after the network connection and security authentication are completed, the Web server is further configured to receive an HTTP request sent by the Web client 11, and send an HTML file to the Web client 11 according to the request;
the Web client 11 is used for receiving an HTML file, analyzing the received HTML file, displaying the HTML file in a Web browser, inputting a temperature measuring and controlling instruction according to the requirement of a user, and sending the instruction to the Web server through the Web browser;
the Web server is further configured to receive an instruction for measuring and controlling the temperature, which is sent by the Web client 11 through the Web browser, perform instruction analysis, and jump to a corresponding instruction response program.
Preferably, the temperature field measurement and control unit 3 comprises N intelligent temperature sensing units 21, a central control single chip microcomputer 15, a synchronous trigger circuit 16, a WiFi module 17, a data signal bus 24 and a temperature control device 4, wherein N is a positive integer;
the N intelligent temperature sensing units 21 are arranged on the microfluidic chip 5 and used for measuring the temperature of each position of the microfluidic chip;
the temperature control signal output end of the central control singlechip 15 is connected with the temperature control signal input end of the temperature control device 4;
the central control single chip microcomputer 15 is connected with the internet of things network transmission system 10 through the WiFi module 17;
the temperature acquisition signal output end of the central control singlechip 15 is connected with the temperature acquisition signal input end of the synchronous trigger circuit 16, and the temperature acquisition signal output end of the synchronous trigger circuit 16 is simultaneously connected with the temperature acquisition signal input ends of the N intelligent temperature sensing units 21;
the N intelligent temperature sensing units 21 are divided into N levels, and the trigger signal output end of each intelligent temperature sensing unit 21 is connected with the trigger signal input end of the next intelligent temperature sensing unit 21;
the temperature data output ends of the N intelligent temperature sensing units 21 are simultaneously connected with the temperature data input end of the data signal bus 24, and the temperature data output end of the data signal bus 24 is connected with the temperature data input end of the central control single chip microcomputer 15;
and the central control singlechip 15 is used for outputting a temperature control signal to the temperature control device 4 according to the temperature control instruction to control the temperature of the microfluidic chip 5, and is also used for outputting a temperature acquisition signal to the synchronous trigger circuit 16 according to the temperature measurement instruction to realize synchronous acquisition and asynchronous output of temperature data to the data signal bus 24 by the N intelligent temperature sensing units 21.
Preferably, the temperature control device 4 comprises a power amplifier circuit 14, a refrigerating device 12 and a heating device 13;
the temperature control signal output end of the central control singlechip 15 is connected with the temperature control signal input end of the power amplifier circuit 14, the refrigeration signal output end of the power amplifier circuit 14 is connected with the refrigeration signal input end of the refrigeration device 12, and the heating signal output end of the power amplifier circuit 14 is connected with the heating signal input end of the heating device 13.
Preferably, the N intelligent temperature sensing units 21 synchronously acquire and asynchronously output temperature data to the data signal bus 24, and further include:
the first-stage intelligent temperature sensing unit 21 is initialized, and after the initialization is completed, when receiving a collecting signal, the first-stage intelligent temperature sensing unit 21 collects a temperature signal, outputs temperature data to the data signal bus 24, and simultaneously outputs a trigger signal to the next-stage intelligent temperature sensing unit 21;
the next-stage intelligent temperature sensing unit 21 is initialized, and after the initialization is completed, when receiving an acquisition signal, the next-stage intelligent temperature sensing unit performs temperature signal acquisition, and when receiving a trigger signal of the previous stage, outputs temperature data to the data signal bus 24, and simultaneously outputs the trigger signal to the next-stage intelligent temperature sensing unit 21;
the last stage of intelligent temperature sensing unit 21 is initialized, and after the initialization is completed, when receiving a collection signal, the temperature signal collection is performed, and when receiving a trigger signal of the previous stage, the temperature data is output to the data signal bus 24, and the collection is finished.
Preferably, each intelligent temperature sensing unit 21 comprises a measurement single chip microcomputer 19, an analog-to-digital converter 20 and a temperature sensor 22;
the temperature sensor is a micro-insertion type temperature sensor 28 or a planar microelectrode temperature sensor;
the private Internet of things system comprises a plurality of micro-insert temperature sensors 28 and a planar microelectrode temperature sensor array 29, wherein the micro-insert temperature sensors 28 are distributed on the top and the side of a micro-channel of a PDMS chip 26 of the micro-fluidic chip 5; the planar microelectrode temperature sensor array 29 is arranged on a substrate 30 of the microfluidic chip 5;
the output end of the planar microelectrode temperature sensor array 29 is connected with the analog-to-digital converter 20 through a lead, the output end of each miniature plug-in temperature sensor 28 is connected with the analog-to-digital converter 20 of the corresponding intelligent temperature sensor through a lead, and the data output end of the analog-to-digital converter 20 of each intelligent temperature sensor is connected with the data input end of the measurement singlechip 19.
Preferably, the temperature field measurement and control unit 3 controls the temperature of the microfluidic chip 5 by using the temperature control device 4 according to the received control temperature command, and further includes:
measuring the temperature of the appointed position of the microfluidic chip 5 or the actual temperature of the temperature field through N intelligent temperature sensors; and comparing the actual temperature with the set temperature in the control temperature instruction, if the temperature difference between the actual temperature and the set temperature is greater than the set maximum temperature difference threshold value, outputting a control signal with a duty ratio of 100% to the temperature control device 4, adjusting the temperature of the microfluidic chip 5 by the temperature control device 4 with the maximum power, otherwise, calling a temperature control algorithm to adjust the duty ratio of the output signal, and correspondingly adjusting the temperature of the microfluidic chip 5 after the temperature control device 4 receives the control signal until the actual temperature is stably maintained at the set temperature.
Preferably, the internet of things network transmission system 10 comprises a wide area network 1, a router 2 and a network penetration module 8;
the router 2 provides an IP address and a port for the temperature field measurement and control unit 3, and the router 2 is connected to the wide area network 1; the network penetration module 8 and the temperature field measurement and control unit 3 are located in the same local area network, and are used for mounting the temperature field measurement and control unit 3 located in the same local area network on the wide area network 1 when the IP address resource of the wide area network 1 is insufficient, the intranet is limited or the operator limits that the IP address cannot be used as a server, so that the Web client 11 is ensured to establish communication connection with the temperature field measurement and control unit 3 through the wide area network 1.
The invention has the beneficial effects that:
1. the system is completely private, the system equipment and the useful information generated by the system are completely private, and the business platform using the Internet of things has potential risks of monitoring, leakage, abuse and the like of the private information;
2. the system has high safety and good information confidentiality, has a private network domain name, is not disclosed to the outside, and has an identity authentication function;
3. the system has wide compatibility and applicability, adopts a B/S mode, can be used as a user terminal by any equipment which is provided with a Web browser and can be connected with a wide area network, allows a plurality of terminals of a user to be online and performs access and control;
4. the high-precision three-dimensional temperature field on-line measurement or remote on-line measurement can be carried out on the micro-fluidic chip;
5. the temperature of the microfluidic chip can be controlled on line or remotely controlled on line;
6. the maintainability of the system is good, the maintenance and the upgrade of the system are simple and convenient, and compared with the commercial platform of the Internet of things, the maintenance and the upgrade are low in difficulty, small in workload and short in time;
7. the system has high expansibility and good portability;
8. the cost is low, and compared with the commercial platform of the Internet of things, the embedded device with low cost is used for development, and both the hardware cost and the program development cost are low.
Drawings
FIG. 1 is a schematic diagram of the system of the present invention;
FIG. 2 is an electrical schematic of the system of the present invention;
FIG. 3 is an electrical schematic of the temperature field measurement and control unit of the present invention;
FIG. 4 is a process flow diagram of the Web server of the present invention;
FIG. 5 is a schematic view of the distribution of the temperature sensors of the present invention;
FIG. 6 is a top view of FIG. 5;
FIG. 7 is a process flow diagram of the first stage smart temperature sensor of the present invention;
FIG. 8 is a process flow diagram of the mid-stage intelligent temperature sensor of the present invention;
FIG. 9 is a process flow diagram of the last stage intelligent temperature sensor of the present invention;
FIG. 10 is a process flow diagram of the temperature field measurement of the present invention;
fig. 11 is a flowchart of the temperature control routine of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that the embodiments and features of the embodiments may be combined with each other without conflict.
The invention is further described with reference to the following drawings and specific examples, which are not intended to be limiting.
The private internet of things system for measuring and controlling the temperature of the microfluidic chip in the embodiment is shown in fig. 2 and comprises a temperature field measurement and control unit 3, an internet of things network transmission system 10 and a Web client 11;
the Web client 11 is connected with the temperature field measurement and control unit 3 through the Internet of things network transmission system 10 and is used for inputting a temperature measurement instruction and a temperature control instruction and sending the temperature measurement instruction to the temperature field measurement and control unit 3;
the temperature field measurement and control unit 3 is used for measuring the temperature or the temperature field of the specified position of the microfluidic chip 5 through the temperature sensor according to the received temperature measurement instruction, and controlling the temperature of the microfluidic chip 5 through the temperature control device 4 according to the received temperature control instruction; a Web server is embedded in the temperature field measurement and control unit 3;
and the internet of things network transmission system 10 is used for establishing and ensuring the network communication connection between the Web client 11 and the temperature field measurement and control unit 3 through the Web server and the wide area network 1.
The temperature field measurement and control unit 3 of the embodiment has the functions of measuring the temperature field of the microfluidic chip, controlling the temperature of the microfluidic chip and embedding the Web server. The Web client 11 is provided with a Web browser and can perform network communication with the intelligent temperature field measurement and control unit through an HTTP (hyper text transport protocol) or an HTTPS (hypertext transfer protocol transport protocol) protocol. The internet of things network transmission system 10 is a link connecting the temperature field measurement and control unit 3 and the Web client 11, and has the functions of giving the temperature field measurement and control unit 3 a socket (IP address plus port) or domain name which can be accessed by the Web client 11 connected to the wide area network 1, smoothly transmitting the service request of the Web client 11 to the temperature field measurement and control unit 3, and smoothly transmitting the response result of the temperature field measurement and control unit 3 to the Web client 11.
The system of the embodiment is completely private, the system equipment and the useful information generated by the system are completely private, and the business platform using the internet of things has potential risks of monitoring, leakage, abuse and the like of the private information.
As shown in fig. 1, the Web client 11 of the present embodiment is composed of a personal computer 7, various smart mobile terminals 6, and other wide area network connectable terminals having a Web browser.
As shown in fig. 2, the internet of things network transmission system 10 in the present embodiment includes a wide area network 1, a router 2, and a network penetration module 8;
the router 2 provides an IP address and a port for the temperature field measurement and control unit 3, and the router 2 is connected to the wide area network 1; the network penetration module 8 and the temperature field measurement and control unit 3 are located in the same local area network, and are used for mounting the temperature field measurement and control unit 3 located in the same local area network on the wide area network 1 when the IP address resource of the wide area network 1 is insufficient, the intranet is limited or the operator limits that the IP address cannot be used as a server, so that the Web client 11 is ensured to establish communication connection with the temperature field measurement and control unit 3 through the wide area network 1.
The system of the embodiment has high safety and good information confidentiality, has a private network domain name, is not open to the outside, and has an identity authentication function, and in the preferred embodiment, as shown in fig. 4, the working flow of the Web server embedded in the temperature field measurement and control unit 3 is as follows: the Web server of the present embodiment is configured to wait for network connection and security authentication of the Web client 11, and after completing the network connection and security authentication, is further configured to receive an HTTP request sent by the Web client 11, and send an HTML file to the Web client 11 according to the request;
the Web client 11 is used for receiving an HTML file, analyzing the received HTML file, displaying the HTML file in a Web browser, inputting a temperature measuring and controlling instruction according to the requirement of a user, and sending the instruction to the Web server through the Web browser;
the Web server is further configured to receive an instruction for measuring and controlling the temperature, which is sent by the Web client 11 through the Web browser, perform instruction analysis, and jump to a corresponding instruction response program.
The system of the embodiment has wide compatibility and applicability, adopts a B/S mode, can be used as a user terminal by any equipment which is provided with a Web browser and can be connected with a wide area network, and allows a plurality of terminals of a user to be online and access and control.
In a preferred embodiment, as shown in fig. 3, the temperature field measurement and control unit 3 of the present embodiment includes N intelligent temperature sensing units 21, a central control single chip microcomputer 15, a synchronous trigger circuit 16, a WiFi module 17, a data signal bus 24, and a temperature control device 4, where N is a positive integer;
the N intelligent temperature sensing units 21 are arranged on the microfluidic chip 5 and used for measuring the temperature of each position of the microfluidic chip;
the temperature control signal output end of the central control singlechip 15 is connected with the temperature control signal input end of the temperature control device 4;
the central control single chip microcomputer 15 is connected with the internet of things network transmission system 10 through the WiFi module 17;
the temperature acquisition signal output end of the central control singlechip 15 is connected with the temperature acquisition signal input end of the synchronous trigger circuit 16, and the temperature acquisition signal output end of the synchronous trigger circuit 16 is simultaneously connected with the temperature acquisition signal input ends of the N intelligent temperature sensing units 21;
the N intelligent temperature sensing units 21 are divided into N levels, and the trigger signal output end of each intelligent temperature sensing unit 21 is connected with the trigger signal input end of the next intelligent temperature sensing unit 21;
the temperature data output ends of the N intelligent temperature sensing units 21 are simultaneously connected with the temperature data input end of the data signal bus 24, and the temperature data output end of the data signal bus 24 is connected with the temperature data input end of the central control single chip microcomputer 15;
and the central control singlechip 15 is used for outputting a temperature control signal to the temperature control device 4 according to the temperature control instruction to control the temperature of the microfluidic chip 5, and is also used for outputting a temperature acquisition signal to the synchronous trigger circuit 16 according to the temperature measurement instruction to realize synchronous acquisition and asynchronous output of temperature data to the data signal bus 24 by the N intelligent temperature sensing units 21.
The synchronous trigger circuit 16 of the present embodiment is composed of a pull-up resistor R, a triode T, and a synchronous signal bus 18; the synchronous trigger circuit 16 receives a control signal from the central control singlechip 15, and simultaneously triggers the first-stage intelligent temperature sensing unit 21, the middle-stage intelligent temperature sensor and the last-stage intelligent temperature sensor to measure the temperature field through the synchronous signal bus 18.
The temperature control device 4 of the present embodiment includes a power amplifier circuit 14, a refrigeration device 12, and a heating device 13;
the temperature control signal output end of the central control singlechip 15 is connected with the temperature control signal input end of the power amplifier circuit 14, the refrigeration signal output end of the power amplifier circuit 14 is connected with the refrigeration signal input end of the refrigeration device 12, and the heating signal output end of the power amplifier circuit 14 is connected with the heating signal input end of the heating device 13.
The working principle of the micro-fluidic chip temperature field measurement of the embodiment is as follows: after receiving a temperature measurement instruction sent by the Web client 11, the temperature field measurement and control unit 3 sends a control signal to the synchronous trigger circuit 16 through the central control single-chip microcomputer 15, the synchronous trigger circuit 16 sends temperature acquisition signals to all the intelligent temperature sensing units 21 mounted on the synchronous signal bus 18, and after receiving the temperature acquisition signals, all the intelligent temperature sensing units 21 convert the temperature signals of the temperature sensors into digital signals through high-precision analog-to-digital converters. And after the central control single chip microcomputer successfully receives the temperature acquisition finishing signal, updating the corresponding temperature sensor value in the HTML file in the embedded Web server program, and sending the updated HTML file to the Web client through the Internet of things network transmission system.
Each intelligent temperature sensing unit 21 of the present embodiment includes a measurement single chip microcomputer 19, an analog-to-digital converter 20, and a temperature sensor 22;
the temperature sensor is a micro-insertion type temperature sensor 28 or a planar microelectrode temperature sensor;
as shown in fig. 5 and 6, the private internet of things system comprises a plurality of micro-insert temperature sensors 28 and a planar microelectrode temperature sensor array 29, wherein the micro-insert temperature sensors 28 are distributed on the top and the side of the micro-channel of the PDMS chip 26 of the microfluidic chip 5; the planar microelectrode temperature sensor array 29 is arranged on a substrate 30 of the microfluidic chip 5;
the output end of the planar microelectrode temperature sensor array 29 is connected to the pin 27 through a planar lead 31 on the substrate 30 and is connected with the analog-to-digital converter 20 through an external lead 32, the output end of each miniature plug-in temperature sensor 28 is connected with the analog-to-digital converter 20 of the corresponding intelligent temperature sensor through an external lead, and the data output end of the analog-to-digital converter 20 of each intelligent temperature sensor is connected with the data input end of the measurement singlechip 19.
In a preferred embodiment, the N intelligent temperature sensing units 21 of this embodiment synchronously acquire and asynchronously output temperature data to the data signal bus 24, and further include:
as shown in fig. 7, the program flow of the first-stage smart temperature sensing unit 21 is: first, the first intelligent temperature sensor is initialized, after the initialization is completed, acquisition signals which are sent by the central control single chip microcomputer 15 and transmitted through the synchronous trigger circuit 16 and the synchronous signal bus 18 are waited, and then the high-precision analog-to-digital converter 20 is controlled to acquire the temperature value of the temperature sensor 22 at the moment. The temperature value is converted into a digital signal, the digital signal is sent to the central control singlechip 15 through a data signal bus 24, and then a trigger signal is sent to a next-stage intelligent temperature sensor, namely a middle-stage intelligent temperature sensor. And finally, waiting for the next acquisition signal sent by the central control singlechip 15.
As shown in fig. 8, first, the middle-stage intelligent temperature sensor is initialized, and after the initialization is completed, the middle-stage intelligent temperature sensor waits for the acquisition signal which is sent by the central control single chip microcomputer 15 and transmitted through the synchronization trigger circuit 16 and the synchronization signal bus 18. Then, the high-precision analog-to-digital converter 20 is controlled to acquire the temperature value of the temperature sensor 22 at this time. After the digital signal is converted into a digital signal, the digital signal waits for the trigger signal to be sent by the upper-level intelligent temperature sensor, namely the first-level intelligent temperature sensor. The intermediate-level intelligent temperature sensor sends the temperature value to the central-control singlechip 15 through the data signal bus 24, and then sends a trigger signal to the next-level intelligent temperature sensor. And finally, waiting for the next acquisition signal sent by the central control singlechip 15.
As shown in fig. 9, the program flow of the last stage intelligent temperature sensor is: firstly, the last stage of intelligent temperature sensor is initialized, and after the initialization is completed, the acquisition signals which are sent by the central control single chip microcomputer 15 and transmitted through the synchronous trigger circuit 16 and the synchronous signal bus 18 are waited. Then, the high-precision analog-to-digital converter 20 is controlled to acquire the temperature value of the temperature sensor at this time. After the digital signal is converted into a digital signal, the trigger signal is waited to be sent by the upper-level intelligent sensor. The last-stage intelligent temperature sensor sends the temperature value and the temperature signal acquisition end signal to the central control single chip microcomputer 15 through a data signal bus 24. And finally, waiting for the next acquisition signal sent by the central control singlechip 15.
As shown in fig. 10, the temperature field measurement program is initialized, and after receiving the instruction for performing the temperature field measurement, the control signal is sent through the synchronous trigger circuit 16 and the synchronous signal bus 18 to control the intelligent temperature sensor mounted on the synchronous signal bus 18 to perform the temperature acquisition, and the acquired temperature information is sequentially transmitted to the central control single chip microcomputer 15 through the data signal bus 24, and after the temperature field measurement is completed, the temperature field measurement program is exited.
In a preferred embodiment, the temperature field measurement and control unit 3 of this embodiment controls the temperature of the microfluidic chip 5 by using the temperature control device 4 according to the received control temperature command, as shown in fig. 11, the temperature control program flow includes:
after receiving a temperature control instruction sent by a Web client, the intelligent temperature field measurement and control unit of the embodiment initializes a temperature control program, and after receiving the instruction for temperature control and related control parameters, the central control single chip microcomputer sends control signals through the synchronous trigger circuit 16 and the synchronous signal bus 18 according to the requirements of the control instruction, controls a designated intelligent temperature sensor mounted on the synchronous signal bus 18 to carry out temperature acquisition, or carries out measurement of a temperature field of the microfluidic chip, and then calculates an actual temperature signal through a temperature determination algorithm, such as an average value algorithm, a median algorithm and the like, or selects a certain specific temperature sensor as an actual temperature. And then, the central control single chip microcomputer updates the numerical value of the corresponding temperature sensor in the HTML file in the embedded Web server program, and sends the updated HTML file to the Web client through the Internet of things network transmission system. Then, the central control singlechip compares the obtained actual temperature with the temperature required to be controlled, and if the difference between the actual temperature and the temperature is larger than a set temperature difference threshold value, a signal with a duty ratio of 100% is output to the power amplification circuit, and the power amplification circuit drives the heating device or the refrigerating device with the maximum power. If the interpolation value of the two temperatures is less than or equal to the set temperature difference threshold value, the central control singlechip calls a temperature control algorithm, such as PID (proportion integration differentiation), Bang-Bang control and the like, to adjust the duty ratio of an output signal, and the output signal controls a heating device or a refrigerating device through a power amplifying circuit until the actual temperature is stably maintained near the set temperature.
Although the invention herein has been described with reference to particular embodiments, it is to be understood that these embodiments are merely illustrative of the principles and applications of the present invention. It is therefore to be understood that numerous modifications may be made to the illustrative embodiments and that other arrangements may be devised without departing from the spirit and scope of the present invention as defined by the appended claims. It should be understood that features described in different dependent claims and herein may be combined in ways different from those described in the original claims. It is also to be understood that features described in connection with individual embodiments may be used in other described embodiments.
Claims (7)
1. A private Internet of things system for measuring and controlling temperature of a microfluidic chip is characterized by comprising:
the Web client (11) is connected with the temperature field measurement and control unit (3) through the Internet of things network transmission system (10) and is used for inputting a temperature measurement and control instruction and sending the instruction to the temperature field measurement and control unit (3);
the temperature field measurement and control unit (3) is used for measuring the temperature or the temperature field of the appointed position of the microfluidic chip (5) through the temperature sensor according to the received temperature measurement instruction, and controlling the temperature of the microfluidic chip (5) through the temperature control device (4) according to the received temperature control instruction; a Web server is embedded in the temperature field measurement and control unit (3);
the internet of things network transmission system (10) is used for establishing and ensuring the network communication connection between the Web client (11) and the temperature field measurement and control unit (3) through the Web server and the wide area network (1);
the temperature field measurement and control unit (3) comprises N intelligent temperature sensing units (21), a central control single chip microcomputer (15), a synchronous trigger circuit (16), a WiFi module (17), a data signal bus (24) and a temperature control device (4), wherein N is a positive integer;
the N intelligent temperature sensing units (21) are arranged on the microfluidic chip (5) and used for measuring the temperature of each position of the microfluidic chip;
the temperature control signal output end of the central control singlechip (15) is connected with the temperature control signal input end of the temperature control device (4);
the central control single chip microcomputer (15) is connected with the internet of things network transmission system (10) through the WiFi module (17);
the temperature acquisition signal output end of the central control single chip microcomputer (15) is connected with the temperature acquisition signal input end of the synchronous trigger circuit (16), and the temperature acquisition signal output end of the synchronous trigger circuit (16) is simultaneously connected with the temperature acquisition signal input ends of the N intelligent temperature sensing units (21);
the N intelligent temperature sensing units (21) are divided into N levels, and the trigger signal output end of each intelligent temperature sensing unit (21) is connected with the trigger signal input end of the lower intelligent temperature sensing unit (21);
the temperature data output ends of the N intelligent temperature sensing units (21) are simultaneously connected with the temperature data input end of the data signal bus (24), and the temperature data output end of the data signal bus (24) is connected with the temperature data input end of the central control single chip microcomputer (15);
and the central control single chip microcomputer (15) is used for outputting a temperature control signal to the temperature control device (4) according to a temperature control instruction to control the temperature of the microfluidic chip (5), and is also used for outputting a temperature acquisition signal to the synchronous trigger circuit (16) according to a temperature measurement instruction to realize synchronous acquisition of the N intelligent temperature sensing units (21) and asynchronous output of temperature data to the data signal bus (24).
2. The private internet of things system of claim 1, wherein the Web server is configured to wait for network connection and security authentication of the Web client (11), and after completing the network connection and security authentication, is further configured to receive an HTTP request sent by the Web client (11), and send an HTML file to the Web client (11) according to the request;
the Web client (11) is used for receiving the HTML file, analyzing the received HTML file and displaying the HTML file in a Web browser, inputting a temperature measuring and controlling instruction according to the requirement of a user, and sending the instruction to the Web server through the Web browser;
the Web server is also used for receiving the instructions for measuring the temperature and controlling the temperature sent by the Web client (11) through the Web browser, analyzing the instructions and jumping to a corresponding instruction response program.
3. The private internet of things system of claim 1, wherein the temperature control device (4) comprises a power amplifier circuit (14), a cooling device (12) and a heating device (13);
the temperature control signal output end of the central control singlechip (15) is connected with the temperature control signal input end of the power amplifier circuit (14), the refrigeration signal output end of the power amplifier circuit (14) is connected with the refrigeration signal input end of the refrigeration device (12), and the heating signal output end of the power amplifier circuit (14) is connected with the heating signal input end of the heating device (13).
4. The private internet of things system of claim 1, wherein said N intelligent temperature sensing units (21) synchronously collect and asynchronously output temperature data to a data signal bus (24), further comprising:
the first-stage intelligent temperature sensing unit (21) is initialized, and after the initialization is finished, when a collecting signal is received, the first-stage intelligent temperature sensing unit (21) collects a temperature signal, outputs temperature data to a data signal bus (24), and simultaneously outputs a trigger signal to the next-stage intelligent temperature sensing unit (21);
the next-stage intelligent temperature sensing unit (21) is initialized, temperature signal acquisition is carried out when an acquisition signal is received after the initialization is finished, temperature data are output to the data signal bus (24) when a trigger signal of the previous stage is received, and meanwhile, the trigger signal is output to the next-stage intelligent temperature sensing unit (21);
and the last stage of intelligent temperature sensing unit (21) is initialized, and after the initialization is finished, when receiving a collecting signal, the temperature signal is collected, and when receiving a trigger signal of the previous stage, the temperature data is output to a data signal bus (24), and the collection is finished.
5. The private internet of things system of claim 3, wherein each intelligent temperature sensing unit (21) comprises a measurement single chip microcomputer (19), an analog-to-digital converter (20) and a temperature sensor;
the temperature sensor is a micro-insertion type temperature sensor (28) or a planar microelectrode temperature sensor;
the private Internet of things system comprises a plurality of micro plug-in temperature sensors (28) and a planar microelectrode temperature sensor array (29), wherein the micro plug-in temperature sensors (28) are distributed on the top and the side of a micro channel of a PDMS chip (26) of the micro-fluidic chip (5); the planar microelectrode temperature sensor array (29) is arranged on a substrate (30) of the microfluidic chip (5);
the output end of the planar microelectrode temperature sensor array (29) is connected with the analog-to-digital converter (20) through a lead, the output end of each miniature plug-in temperature sensor (28) is connected with the analog-to-digital converter (20) of the corresponding intelligent temperature sensor through a lead, and the data output end of the analog-to-digital converter (20) of each intelligent temperature sensor is connected with the data input end of the measurement singlechip (19).
6. The private internet of things system of claim 1, wherein the temperature field measurement and control unit (3) controls the temperature of the microfluidic chip (5) by using the temperature control device (4) according to the received control temperature command, further comprising:
measuring the temperature of the appointed position of the microfluidic chip (5) or the actual temperature of the temperature field through N intelligent temperature sensors; and comparing the actual temperature with the set temperature in the control temperature instruction, if the temperature difference between the actual temperature and the set temperature is greater than the set maximum temperature difference threshold, outputting a control signal with a duty ratio of 100% to the temperature control device (4), adjusting the temperature of the microfluidic chip (5) by the temperature control device (4) with the maximum power, otherwise, calling a temperature control algorithm to adjust the duty ratio of the output signal, and correspondingly adjusting the temperature of the microfluidic chip (5) by the temperature control device (4) after receiving the control signal until the actual temperature is stably maintained at the set temperature.
7. The private internet of things system of claim 1, wherein the internet of things network transmission system (10) comprises a wide area network (1), a router (2), and a network penetration module (8);
the router (2) provides an IP address and a port for the temperature field measurement and control unit (3), and the router (2) is connected to the wide area network (1); the network penetration module (8) and the temperature field measurement and control unit (3) are located in the same local area network and used for mounting the temperature field measurement and control unit (3) located in the same local area network on the wide area network (1) when the IP address resource of the wide area network (1) is insufficient and the intranet limitation or the operator limitation IP address cannot be used as a server, and the Web client (11) is ensured to establish communication connection with the temperature field measurement and control unit (3) through the wide area network (1).
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| CN111266139B (en) * | 2020-03-02 | 2022-06-07 | 京东方科技集团股份有限公司 | Temperature control system, detection system and temperature control method of micro-fluidic chip |
| CN111495295B (en) * | 2020-04-24 | 2021-11-19 | 太原理工大学 | Method for detecting temperature field in microreactor and microreactor |
| CN111830933B (en) * | 2020-07-16 | 2022-03-29 | 广东亨通光电科技有限公司 | Heating element detection system, method and device and storage medium |
| TWI810575B (en) * | 2021-03-19 | 2023-08-01 | 國立陽明交通大學 | Microelectrode device, microfluidic chip, and microfluidic examination method |
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