CN110470511B - Manual sample injector for sample pretreatment, manual sample injection method and application thereof - Google Patents
Manual sample injector for sample pretreatment, manual sample injection method and application thereof Download PDFInfo
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- 238000002347 injection Methods 0.000 title claims abstract description 23
- 239000007924 injection Substances 0.000 title claims abstract description 23
- 238000000034 method Methods 0.000 title claims abstract description 16
- 238000004458 analytical method Methods 0.000 claims description 34
- 238000001914 filtration Methods 0.000 claims description 20
- 238000006243 chemical reaction Methods 0.000 claims description 17
- 238000007789 sealing Methods 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 9
- 239000000741 silica gel Substances 0.000 claims description 6
- 229910002027 silica gel Inorganic materials 0.000 claims description 6
- 150000001805 chlorine compounds Chemical class 0.000 claims description 5
- DOBUSJIVSSJEDA-UHFFFAOYSA-L 1,3-dioxa-2$l^{6}-thia-4-mercuracyclobutane 2,2-dioxide Chemical compound [Hg+2].[O-]S([O-])(=O)=O DOBUSJIVSSJEDA-UHFFFAOYSA-L 0.000 claims description 4
- 239000003153 chemical reaction reagent Substances 0.000 claims description 4
- 239000003365 glass fiber Substances 0.000 claims description 4
- 229910000370 mercury sulfate Inorganic materials 0.000 claims description 3
- 238000002203 pretreatment Methods 0.000 claims 2
- 230000008569 process Effects 0.000 abstract description 6
- 238000010979 pH adjustment Methods 0.000 description 7
- 238000007781 pre-processing Methods 0.000 description 7
- 238000001514 detection method Methods 0.000 description 6
- 239000004743 Polypropylene Substances 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 239000000835 fiber Substances 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- -1 polypropylene Polymers 0.000 description 4
- 229920001155 polypropylene Polymers 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 239000003344 environmental pollutant Substances 0.000 description 3
- 239000012535 impurity Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 239000010865 sewage Substances 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- 241000282414 Homo sapiens Species 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002032 lab-on-a-chip Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 229940074994 mercuric sulfate Drugs 0.000 description 1
- 229910000372 mercury(II) sulfate Inorganic materials 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 1
- 125000005575 polycyclic aromatic hydrocarbon group Chemical group 0.000 description 1
- 239000004926 polymethyl methacrylate Substances 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 239000010453 quartz Substances 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
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- 239000013589 supplement Substances 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention relates to the field of sample injectors, and discloses a manual sample injector for sample pretreatment, a sample injection method and application thereof, wherein the manual sample injector comprises a piston, a sample cell, a pretreatment part and a sample outlet part, the pretreatment part is detachably and hermetically connected with the sample cell, the pretreatment part is connected with the sample outlet part, and the pretreatment part is used for pretreatment of samples; the piston seals the sample cell to form a sample cavity, the sample outlet part is positioned on one side of the pretreatment part opposite to the piston, the volume of the sample cavity can be changed through the movement of the piston, and the sample in the sample cavity passes through the pretreatment part and is extruded through the sample outlet part. The pretreatment part is arranged in the manual sample injector, so that the pretreatment process is finished while the sample is added, the operation of independently carrying out pretreatment is omitted, the device is simplified, and the time is saved.
Description
Technical Field
The invention relates to the field of sample injectors, in particular to a manual sample injector for sample pretreatment, a manual sample injection method and application thereof.
Background
The world economy and industry development brings great convenience to human beings, and simultaneously, the emission of pollutants is continuously increased in quantity and variety, so that the environmental protection problem is becoming serious. The small portable, simple and rapid analysis technology becomes a big research hot spot while various high-precision analysis instruments and detection technologies are greatly developed, so as to supplement the defects of complicated operation, large maintenance workload, high requirements on laboratory environment, difficulty in being used for field detection and the like of the large instrument.
The micro total analysis system is also called a lab-on-a-chip, is an analysis technology aimed at integrating all functions of a laboratory on one chip, and has shown excellent prospects in terms of miniaturization, portability and automation of the analysis technology, and is a big research hotspot in recent years.
The micro full analysis system is still in the development stage at present, and the function of a chip laboratory cannot be actually realized, so that when the analysis experiment of pretreatment is required, the pretreatment part experiment is usually finished manually, and then the micro full analysis system is entered for analysis, so that the development of automation, rapidness and portability of the micro full analysis system is limited.
Disclosure of Invention
The invention aims to solve the problems of complex pretreatment operation, time consumption and the like in the prior art, and provides a manual sample injector and a manual sample injection method for sample pretreatment.
In order to achieve the above object, the present invention provides a manual sample injector for sample pretreatment, wherein the manual sample injector comprises a piston, a sample cell, a pretreatment part and a sample outlet part, the pretreatment part is detachably and hermetically connected with the sample cell, the pretreatment part is connected with the sample outlet part, and the pretreatment part is used for pretreatment of a sample; the piston seals the sample cell to form a sample cavity, the sample outlet part is positioned on one side of the pretreatment part opposite to the piston, the volume of the sample cavity can be changed through the movement of the piston, and the sample in the sample cavity passes through the pretreatment part and is extruded through the sample outlet part.
Preferably, the sample cell is detachably connected to the pretreatment part by a plug-in part, a thread, a buckle or a groove.
Preferably, the preprocessing section includes 1 or more preprocessing modules.
Preferably, the pretreatment module is selected from one or more of a filtration module, an enrichment module, a reaction module, a separation module, and a pH adjustment module.
Preferably, the pretreatment module comprises a filtration module, a pH adjustment module, and a reaction module.
Preferably, more than 2 pretreatment modules are detachably connected in a sealing way.
Preferably, a sample inlet is arranged at the top of the pretreatment module, a sample outlet is arranged at the bottom of the pretreatment module and the bottom of the sample cell, and the sample inlet and the sample outlet are detachably and hermetically connected with each other.
Preferably, the sample outlet portion is provided in an intermediate portion of the pretreatment portion on a side opposite to the piston.
Preferably, the sample cell, the piston and the pretreatment portion are all cylindrical.
Preferably, the sample cell is a transparent or semitransparent sample cell, and the pretreatment part is a transparent or semitransparent pretreatment part.
Preferably, the piston forms a sleeve structure with the sample cell.
Preferably, the piston is screwed or connected to the sample cell by an elastic sealing member.
The invention also provides a manual sample injection method, which comprises the following steps: and moving the piston of the manual sample injector to enable the sample in the sample cavity to pass through the pretreatment part and be extruded out through the sample outlet part.
The invention also provides application of the manual sample injector or the manual sample injection method in the fields of environmental pollutant detection, in-vitro diagnosis or micro total analysis chips and the like.
Through the technical scheme, the manual sample injector for sample pretreatment has the following advantages:
1) The pretreatment operation can be completed while sample injection is performed, so that the automation, the rapidness and the portability of the micro-total analysis system are greatly facilitated;
2) The pretreatment operation and the use of external equipment are reduced, and the volume and the complexity of the micro total analysis system are greatly reduced;
3) The pre-reaction modules are mutually independent and detachable and can be assembled or disassembled at any time according to the needs;
4) By preprocessing the sample, non-reactive substances are prevented from entering the chip, so that the analysis result is more accurate;
5) The pretreatment parts with different interfaces are replaced, so that the device can be conveniently matched with chips of various types for use, and the application range of the manual sample injector is widened.
Drawings
FIG. 1 is a schematic diagram of a manual injector for sample pretreatment according to the present invention;
FIG. 2 is a schematic diagram of a pretreatment module according to the present invention
Description of the reference numerals
1. Piston 2, sample cell
3. Pretreatment section 4, sample discharge section
Detailed Description
The endpoints and any values of the ranges disclosed herein are not limited to the precise range or value, and are understood to encompass values approaching those ranges or values. For numerical ranges, one or more new numerical ranges may be found between the endpoints of each range, between the endpoint of each range and the individual point value, and between the individual point value, in combination with each other, and are to be considered as specifically disclosed herein.
In the present invention, unless otherwise indicated, terms of orientation such as "upper, lower, left, right" are used to refer generally to the upper, lower, left, right of the drawing.
The invention provides a manual sample injector for sample pretreatment, as shown in figures 1-2, wherein the manual sample injector comprises a piston 1, a sample cell 2, a pretreatment part 3 and a sample outlet part 4, wherein the pretreatment part 3 is detachably and hermetically connected with the sample cell 2, the pretreatment part 3 is connected with the sample outlet part 4, and the pretreatment part 3 is used for pretreating a sample; the piston 1 seals the sample cell 2 to form a sample cavity, the sample outlet 4 is positioned on the side of the pretreatment part 3 opposite to the piston 1, the volume of the sample cavity can be changed by moving the piston 1, and the sample in the sample cavity passes through the pretreatment part 3 and is extruded through the sample outlet 4.
By using the manual sample injector for sample pretreatment, the pretreatment of the samples can be carried out simultaneously in the sample injection process, so that the operation of pretreatment operation is greatly simplified, and the sample injection under a complex operation scene is facilitated. The use mode of the manual sample injector is not particularly limited, and in the sample injection process, the lower part in fig. 1 can be used towards any direction, and sample pretreatment and sample injection operation can be completed.
As the sample pretreatment in the present invention, filtration, enrichment, separation, pH adjustment, reaction, and the like can be mentioned. For example, the pretreatment of the sewage sample requires operations such as filtration and pH adjustment in sequence; the pretreatment of the sample with lower target content needs to be sequentially performed with operations such as filtration, enrichment and the like.
According to the present invention, the pretreatment unit 3 preferably includes 1 or more pretreatment modules, preferably 2 or more, more preferably 2 to 5, and even more preferably 2 to 3 pretreatment modules, in order to cope with various pretreatment operations required for the sample.
As the pretreatment operation performed in each pretreatment module, selection can be made according to the sample to be introduced. Specifically, the pretreatment module is selected from one or more of a filtration module, an enrichment module, a separation module, a pH adjustment module, and a reaction module. As the filtration module, there may be provided therein fibers, porous solids, a stacking medium, etc., for example, one or more of polypropylene fibers, glass fibers, activated carbon, gravel, among which polypropylene fibers, glass fibers are preferable; as an enrichment module, alumina, silica, targeting vectors can be provided therein; as a reaction module, a desired reactant, such as mercury sulfate, which is precipitated with chloride, may be included.
For example, when pretreatment of a phosphate measurement sample in sewage is performed, the pretreatment module comprises a filtration module, a pH adjustment module and a pre-reaction module; when the pretreatment of the sample for measuring the polycyclic aromatic hydrocarbon in the water is carried out, the pretreatment module comprises a filtration module and an enrichment module. As shown in fig. 1, the pretreatment unit 3 includes a first pretreatment module 3a, a second pretreatment module 3b, and a third pretreatment module 3c, which are respectively a filtration module, a pH adjustment module, and a reaction module when the pretreatment unit is used for pretreating a phosphate measurement sample in sewage.
According to the invention, by detachably connecting the pretreatment part 3 with the sample cell 2, different pretreatment modules can be conveniently arranged, so that the manual sampler meets the requirements of pretreatment and sample introduction of different samples, and can be conveniently matched with different devices to be added with samples, such as a micro total analysis chip and a micro fluidic chip. The pretreatment unit 3 may be connected to the bottom of the sample cell 2, or may be provided inside the sample cell 2, and is preferably connected to the bottom of the sample cell 2.
As a means of removable connection, the cuvette 2 may be removably connected to the pretreatment section 3 by means of a plug-in section, a screw thread, a snap-in or a groove. When the connection is made by threads, a sample outlet matching with the sample inlet of the pretreatment unit 3 shown in fig. 2 may be provided at the bottom of the sample cell 2, for example, the sample outlet may be tubular, an external thread may be provided at the sample outlet, and a sample inlet matching with the sample outlet may be provided at the top of the pretreatment unit 3, and an internal thread may be provided at the sample inlet. The sample inlet and the sample outlet are then screwed together, whereby the pretreatment portion 3 is detachably connected to the sample cell 2. When the connection is performed through the buckle, the bottom of the sample cell 2 and the top of the pretreatment part 3 can be respectively provided with the matched buckle, and the buckle can be arranged on the side wall and the connection surface of the sample cell 2 and the pretreatment part 3. The structure of the above-described catch is not particularly limited as long as the pretreatment unit 3 is detachably connected to the sample cell 2.
According to the present invention, when the pretreatment unit 3 includes more than 2 pretreatment modules, preferably more than 2 pretreatment modules are detachably connected in a sealing manner, so that different pretreatment processes can be freely combined. The pretreatment modules may be detachably connected to each other in the same manner as the pretreatment unit 3 and the sample cell 2. Specifically, a sample inlet may be provided at the top of the pretreatment module, and a sample outlet may be provided at the bottoms of the pretreatment module and the sample cell, and the sample inlet and the sample outlet may be detachably connected to each other in a sealing manner. Thus, the above-mentioned plug-in parts, threads, buckles or grooves and the like can be arranged between the pretreatment modules and between the pretreatment part 3 and the sample cell 2, for example, at the sample inlet and the sample outlet, so that the sample cell 2 and the pretreatment modules can be detachably connected.
In order to improve the sealability between the sample cell 2 and the pretreatment portion 3, for example, a sealing performance between the connection portion of the sample cell 2 and the pretreatment portion 3 may be provided, and a sealing auxiliary member such as a sealing gasket may be further provided between the two according to need, thereby improving the sealability of the sample injector and ensuring smooth progress of the process.
According to the present invention, the shapes of the sample cell 2, the piston 1 and the pretreatment portion 3 are not particularly limited, and are preferably each cylindrical, more preferably cylindrical. The sample cell 2 and the piston 1 may be formed in a sleeve structure, wherein the piston 1 may be an outer cylinder or an inner cylinder. From the viewpoint of facilitating sample application, the piston 1 is preferably used as an inner cylinder, and the sample cell 2 is preferably used as an outer cylinder. For convenience of sample injection, the top of the piston 1 may have a larger area of end, so that the piston 1 is conveniently pushed manually.
According to a preferred embodiment of the invention, the piston 1 is screwed or connected to the cuvette 2 by means of an elastic sealing member.
According to the present invention, the position where the sample outlet portion 4 is provided is not particularly limited, and the sample outlet portion 4 is provided in the middle portion of the pretreatment portion 3 on the side opposite to the piston 1.
According to the invention, in order to ensure that the sample is completely introduced into the device to be loaded (for example, a micro-total analysis chip) during loading, it is preferable to have the loading portion in sealing connection with the device to be loaded, in particular, the loading portion 4 may be provided with a threaded structure or an elastic sealing member, so that the interface portion may be screwed or sealed by the loading portion with the device to be loaded.
The thread structure can be an internal thread structure or an external thread structure, and the thread structure can be in sealing connection with a device to be loaded. The flexible sealing component can be a rubber plug and the like, and can specifically enable the sample outlet part to be arranged inside the rubber plug, and the sample inlet of the device to be added with samples is sealed through the rubber plug when sealing connection is completed.
According to a preferred embodiment of the present invention, in order to secure the seal between the piston 1 and the cuvette 2, for example, a sanding treatment may be performed at the portion where the piston 1 and/or the cuvette 2 are connected; a soft sealing member (such as a gasket made of an inert rubber/silica gel or the like) may be provided at the bottom of the piston 1.
According to the present invention, in order to facilitate observation of the sample introduction condition of the sample, the sample cell 2 is a transparent or semitransparent sample cell, and the pretreatment portion 3 is a transparent or semitransparent pretreatment portion. The material of the sample cell 2 and the sample cell 1 may be, for example, one or more selected from glass, polypropylene, quartz, polymethyl methacrylate, and polystyrene. Among them, glass is preferable. The piston 1 and the sample tank 2 are transparent or semitransparent, so that the states of liquid and gas in the sample tank can be observed and mastered at any time, and the sample adding process can be controlled conveniently.
The invention also provides a manual sample injection method, which comprises the following steps: the piston 1 of the manual sample injector is moved, so that the sample in the sample cavity passes through the pretreatment part 3 and is extruded out through the sample outlet part 4. The manual sample injector is used for sample injection, so that the pretreatment of the sample can be finished at the same time of sample injection, the pretreatment and sample injection operation are simplified, and the requirements of a portable detection system are met; and the preprocessing part 3 can select different preprocessing modules, and can freely combine preprocessing steps required to be performed, so as to adapt to the preprocessing requirements of different samples.
The invention also provides application of the manual sample injector or the manual sample injection method in the fields of environmental pollutant detection, in-vitro diagnosis or micro total analysis chips and the like.
The present invention will be described in detail by examples.
Example 1
This example illustrates a manual sample injector for performing a filtering pretreatment.
The manual sampler comprises a piston 1, a sample cell 2, a pretreatment part 3 and a sample outlet part 4, wherein the piston 1, the sample cell 2 and the pretreatment part 3 are all made of cylindrical and transparent glass materials.
The pretreatment unit 3 is a filter module. The structure of the filter module is shown in fig. 2, and a polypropylene fiber filter disc is arranged in the filter module; the top of the device is provided with a sample inlet, and the side wall of the sample inlet is provided with internal threads. The bottom of the sample cell 2 is provided with a sample outlet, the side wall of the sample outlet is provided with external threads, the sample inlet of the pretreatment part 3 is screwed with the sample outlet of the sample cell 2, and the pretreatment part 3 is detachably and hermetically connected with the sample cell 2.
The sample outlet of the pretreatment part 3 is used as a sample outlet part 4, the sample outlet part 4 is positioned at the middle part of the bottom of the pretreatment part 3, and the side wall of the sample outlet part is provided with external threads for screwing with a micro full analysis chip.
The piston 1 can seal the sample cell 2 to form a sample cavity, the volume of the sample cavity can be changed by manually pushing the piston 1, and the sample in the sample cavity passes through the pretreatment part 3 and is extruded out through the sample outlet part 4.
Before starting sample injection, the piston 1 is opened, 1mL of turbid water sample is injected into the sample injector, the piston 1 is closed, the sample injector is screwed with the sample inlet of the micro-total analysis chip, then the piston 1 is pushed to reduce the volume of the sample cavity, and the sample is filtered by the filter disc of the pretreatment part 3 and then enters the micro-total analysis chip. And observing the water sample entering the chip, and no granular impurities are found.
Example 2
The present example is to illustrate a manual sample injector for performing enrichment pretreatment.
The same manual injector as in example 1 was used, except that the pretreatment section 3 was an enrichment module, inside of which functional silica gel for enriching the organic matters in the sample was provided.
Before starting sample injection, opening a piston 1, injecting 1mL of turbid water sample into a sample injector, closing the piston 1, screwing the sample injector and a sample inlet of a micro full analysis chip, pushing the piston 1 to reduce the volume of a sample cavity, and allowing a sample to enter the micro full analysis chip for parameter analysis after the sample is enriched with organic matters through functional silica gel of a pretreatment part 3.
The sample injector is taken down from the micro full analysis chip and is arranged on another micro full analysis chip, the piston 1 is opened, a certain amount of organic solvent such as methanol is injected into the sample cell, the piston is covered again and is pushed downwards in a rotating mode, and the organic matters enriched in the silica gel module are washed to enter the micro full analysis chip, so that the enriched organic matters can be analyzed.
Example 3
This example illustrates a manual sample injector for performing a pretreatment reaction.
The same manual sample injector as in example 1 was used, except that the pretreatment section 3 was a reaction reagent module, the inside of which was provided with a mercury sulfate reagent for removing chlorides in the water sample, which was closed by a sieve plate.
Before starting sample injection, opening a piston 1, injecting 1mL of turbid water sample into a sample injector, wherein the water sample contains oxygen-consuming organic matters to be detected (the detection index is COD), closing the piston 1, screwing the sample injector and a sample inlet of a micro full analysis chip, pushing the piston 1 to reduce the volume of a sample cavity, enabling a sample to be detected to enter a pretreatment part under the action of pressure, sequentially removing solid impurities in water through a filtering module, removing chlorides in the water sample through a reaction module, and entering the micro full analysis chip for analysis.
Example 4
This example illustrates a manual sample injector for filtration, enrichment and reaction pretreatment.
The manual sample injector shown in fig. 1 is used, and comprises a piston 1, a sample cell 2, a pretreatment part 3 and a sample outlet part 4, wherein the piston 1, the sample cell 2 and the pretreatment part 3 are all made of cylindrical and transparent glass materials.
The pretreatment part 3 comprises a filtering module 3a, an enrichment module 3b and a reaction module 3c. The structure of each module is shown in fig. 2, glass fibers are arranged inside the filtering module 3a, functional silica gel for enriching organic matters in the water body is arranged inside the enriching module 3b, and a mercuric sulfate reagent for removing chlorides in the sample is arranged inside the module 3c. The top of each module is provided with a sample inlet with internal threads on the side walls of the sample inlet. The bottom of each module and the sample cell 2 are respectively provided with a sample outlet, the side wall of each sample outlet is provided with external threads, and the filtering module 3a, the enriching module 3b and the reacting module 3c of the pretreatment part 3 are connected in a pair-by-pair screwing way in sequence, so that the pretreatment part 3 is detachably connected with the sample cell 2.
The sample outlet of the pretreatment part 3 is used as a sample outlet part 4, the sample outlet part 4 is positioned at the middle part of the bottom of the pretreatment part 3, and the side wall of the sample outlet part is provided with external threads for screwing with a micro full analysis chip.
The piston 1 can seal the sample cell 2 to form a sample cavity, the volume of the sample cavity can be changed by manually pushing the piston 1, and the sample in the sample cavity passes through the pretreatment part 3 and is extruded out through the sample outlet part 4.
Before starting sample injection, opening a piston 1, injecting 1mL of turbid water sample into a sample injector, closing the piston 1, screwing the sample injector and a sample inlet of the micro full analysis chip, pushing the piston 1 to reduce the volume of a sample cavity, and sequentially removing solid impurities, adsorbing and enriching organic matters and removing chlorides in water by a filtering module 3a, an enriching module 3b and a reaction module 3c of a pretreatment part 3 to obtain the sample, wherein the sample enters the micro full analysis chip.
The preferred embodiments of the present invention have been described in detail above, but the present invention is not limited thereto. Within the scope of the technical idea of the invention, a number of simple variants of the technical solution of the invention are possible, including combinations of the individual technical features in any other suitable way, which simple variants and combinations should likewise be regarded as being disclosed by the invention, all falling within the scope of protection of the invention.
Claims (7)
1. The application of the manual sample injector in the field of micro total analysis chips is characterized in that the manual sample injector comprises a piston (1), a sample cell (2), a pretreatment part (3) and a sample outlet part (4),
The pretreatment part (3) is detachably connected with the sample pool (2) in a sealing way, the pretreatment part (3) is connected with the sample outlet part (4), and the pretreatment part (3) is used for carrying out pretreatment on a sample;
The piston (1) seals the sample pool (2) to form a sample cavity, the sample outlet part (4) is positioned on one side of the pretreatment part (3) opposite to the piston (1), the volume of the sample cavity can be changed through the movement of the piston (1), and a sample in the sample cavity passes through the pretreatment part (3) and is extruded through the sample outlet part (4);
the pretreatment part (3) comprises a filtering module (3 a), an enrichment module (3 b) and a reaction module (3 c); glass fibers are arranged in the filtering module (3 a), functional silica gel for enriching organic matters in the water body is arranged in the enriching module (3 b), and a mercury sulfate reagent for removing chlorides in the sample is arranged in the reaction module (3 c);
Sample outlets are formed in the bottoms of the sample pool (2) and each module, external threads are formed in the side wall of the sample outlet, and the filtering module (3 a), the enrichment module (3 b) and the reaction module (3 c) of the pretreatment part (3) are connected in a screwed mode in sequence in pairs, so that the pretreatment part (3) is detachably connected with the sample pool (2);
The sample outlet of the pretreatment part (3) is used as a sample outlet part (4), the sample outlet part (4) is positioned at the middle part of the bottom of the pretreatment part (3), and the side wall of the sample outlet is provided with external threads for screwing with a micro full analysis chip.
2. Use according to claim 1, wherein the sample outlet (4) is provided in the middle of the pretreatment portion (3) on the side opposite to the piston (1).
3. Use according to claim 1, wherein the sample cell (2), the piston (1) and the pre-treatment section (3) are all cylindrical.
4. Use according to claim 1, wherein the sample cell (2) is a transparent or translucent sample cell and the pretreatment (3) is a transparent or translucent pretreatment.
5. Use according to any of claims 1-4, wherein the piston (1) forms a sleeve structure with the sample cell (2).
6. Use according to claim 5, wherein the piston (1) is screwed or connected to the sample cell (2) by means of an elastic sealing member.
7. The manual sample injection method is characterized by comprising the following steps of: a plunger (1) of a manual sample injector according to any of claims 1-6 is moved such that the sample in the sample chamber passes through the pre-treatment section (3) and is pressed out through the sample outlet section (4).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810448768.3A CN110470511B (en) | 2018-05-11 | 2018-05-11 | Manual sample injector for sample pretreatment, manual sample injection method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810448768.3A CN110470511B (en) | 2018-05-11 | 2018-05-11 | Manual sample injector for sample pretreatment, manual sample injection method and application thereof |
Publications (2)
| Publication Number | Publication Date |
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| CN110470511A CN110470511A (en) | 2019-11-19 |
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| CN100542666C (en) * | 2007-07-25 | 2009-09-23 | 浙江大学 | A kind of solid-liquid reaction tanks and application thereof with gas separation and collecting function |
| CN202870104U (en) * | 2012-06-28 | 2013-04-10 | 中国人民解放军军事医学科学院卫生学环境医学研究所 | Manual air-pressure sample-injecting device for micro-fluidic chip |
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