CN110302307B

CN110302307B - Yin-nourishing and beauty-maintaining medicinal liquor for women and preparation method thereof

Info

Publication number: CN110302307B
Application number: CN201910659190.0A
Authority: CN
Inventors: 窦金金; 张喜武; 周琦; 杨斯棋
Original assignee: Heilongjiang University of Chinese Medicine
Current assignee: Heilongjiang University of Chinese Medicine
Priority date: 2019-07-22
Filing date: 2019-07-22
Publication date: 2021-06-25
Anticipated expiration: 2039-07-22
Also published as: CN110302307A

Abstract

The invention discloses yin-nourishing, beautifying and liver-protecting medicinal liquor for women, which comprises the following components: clove leaves, clove, mulberry, radix asparagi, prepared rehmannia root, acanthopanax, medlar, Chinese date and wine. The invention also discloses a preparation method of the female medicinal liquor for nourishing yin, beautifying features and protecting liver. According to the yin-nourishing, beautifying and liver-protecting female medicinal liquor and the preparation method thereof, the optimal development method is obtained by optimizing the proportioning and percolation process flow of the female medicinal liquor formula through an orthogonal design test, so that the female medicinal liquor can better exert the curative effect. The proportion of 4 main drugs of clove leaves, mulberry, asparagus cochinchinensis and prepared rehmannia root in the formula is optimized by sensory evaluation, the percolation process is optimized by taking the content of crude polysaccharide as an evaluation index, and finally the optimal proportion of the components of the medicinal liquor for nourishing yin, beautifying and protecting liver for women and the optimal process of the percolation process are obtained.

Description

Yin-nourishing and beauty-maintaining medicinal liquor for women and preparation method thereof

Technical Field

The invention relates to female medicinal liquor for nourishing yin, beautifying features and protecting liver and a preparation method thereof, belonging to the technical field of traditional Chinese medicines.

Background

According to the traditional Chinese medicine theory of China, the wine is one of hundreds of medicines, and the traditional Chinese medicine curative effect can be better increased by reasonable use. The medicinal liquor has been used for thousands of years in China and is a very important component in Chinese medical science.

Nowadays, with the continuous development of the economic level of China, the continuous improvement of the living standard of residents and the change of social economy and living environment, the consciousness of human beings on health concept and living quality pursuit is greatly enhanced, and in the face of the strengthening of working efficiency, the fierce competition and the indispensable wine table culture on the workplace, how to realize the pressure relief and the social compensation under the condition of no or little body injury is a problem which needs to be solved urgently at present.

Disclosure of Invention

The invention aims to solve the technical problem of providing the female medicinal liquor for nourishing yin, beautifying and protecting liver aiming at the problems in the prior art so as to overcome the defects in the prior art.

Meanwhile, the preparation method of the female medicinal liquor for nourishing yin, beautifying features and protecting liver is provided by the invention, the medicinal liquor prepared by the method is scientific and reasonable in formula proportion, simple and convenient in operation method, easy in result arrangement, and stable and reliable in optimized percolation process result, and can provide an important basis for further developing the female medicinal liquor.

In order to solve the technical problems, the technical scheme adopted by the invention is as follows:

female medicinal liquor for nourishing yin, beautifying features and protecting liver comprises the following components: clove leaves, clove, mulberry, radix asparagi, prepared rehmannia root, acanthopanax, medlar, Chinese date and wine.

Female medicinal liquor for nourishing yin, beautifying features and protecting liver comprises the following components in parts by weight: 0.2-0.4 part of clove leaf, 2.5-5 parts of clove, 5-6 parts of mulberry, 5-6 parts of radix asparagi, 2.5-5 parts of prepared rehmannia root, 2.5-5 parts of acanthopanax, 2.5-5 parts of medlar, 5-10 parts of Chinese date and 400-600 parts of wine.

Female medicinal liquor for nourishing yin, beautifying features and protecting liver comprises the following components: 0.2g of clove leaf, 2.5g of clove, 5g of mulberry, 5g of radix asparagi, 2.5g of prepared rehmannia root, 2.5g of acanthopanax, 2.5g of medlar, 5g of Chinese date and 500mL of wine, wherein the proportion can be increased or decreased in the same proportion.

The wine is 50-55-degree white spirit.

A preparation method of female medicinal liquor for nourishing yin, beautifying features and protecting liver comprises the following steps:

the method comprises the following steps: taking a proper amount of clove leaves, clove, mulberry, asparagus cochinchinensis, prepared rehmannia root, acanthopanax, medlar and Chinese date, and respectively crushing to 20-60 meshes to obtain a medicinal material mixture for later use;

step two: dividing wine into two parts for later use;

step three: putting the medicinal material mixture prepared in the step one into a container, adding a part of the wine prepared in the step two, and soaking the medicinal material mixture for at least 24 hours to ensure that the medicinal material mixture is fully expanded to prepare an expanding medicine;

step four: placing absorbent cotton into the container in the third step for wetting, and then paving the absorbent cotton at the bottom of the percolation cylinder;

step five: placing the swelling medicine in the third step on the top of the wetted absorbent cotton, placing filter paper or gauze on the top of the swelling medicine, and placing a heavy object on the top of the filter paper or the gauze;

step six: closing a piston at the outlet at the lower end of the percolation cylinder, slowly adding the other part of the wine in the step two into the top of the percolation cylinder, sealing the percolation cylinder by a cover for a period of time, and opening the piston at the outlet at the lower end of the percolation cylinder to enable the percolation liquid to slowly percolate at the flow rate of 0.8-1 ml/min;

step seven: collecting the percolate obtained in the sixth step, slowly adding crystal sugar after the percolate is collected, and stirring to completely dissolve the crystal sugar to obtain a semi-finished product of the medicinal liquor;

step eight: adding a clarifying agent in the standing and precipitating period of the semi-finished product of the medicinal liquor, standing and precipitating for at least 20 days, and filtering;

step nine: filtering the semi-finished product of the medicated wine prepared in the step eight by using filter cloth, and collecting filtrate, wherein the mesh number of the filter cloth is 120-150 meshes;

step ten: and (4) after the required wine bottle is sterilized, filling the lady medicinal liquor prepared in the step nine into the wine bottle to finish filling.

And step eight can be replaced by a low-temperature impurity removal method.

The low-temperature impurity removal method comprises the following steps: placing the semi-finished product of medicated liquor after standing and precipitating in a freezing device, stopping cooling when the temperature of the semi-finished product of medicated liquor is reduced to-15 deg.C, taking out the semi-finished product of medicated liquor, naturally raising the temperature to 5 deg.C, and filtering.

The clarifying agent is diatomite which is 0.05-0.1% of the weight of the medicinal material mixture.

The clarifying agent comprises a protein flocculating agent, an amphoteric polyacrylamide flocculating agent and a 101 fruit juice clarifying agent, and the dosage of the clarifying agent is 0.03-0.05% of the weight of the medicinal material mixture.

The ratio of the protein flocculant to the amphoteric polyacrylamide flocculant to the 101 fruit juice clarifying agent is 2:1: 2.

In the invention, the mulberry, the clove leaves and the clove have the effects of protecting liver and relieving alcoholism; radix asparagi and radix rehmanniae Preparata have effects of nourishing yin and caring skin; the Chinese dates and the Chinese wolfberry have the effects of resisting aging and oxidation; the acanthopanax root has the efficacy of improving sleep, and the female medicinal liquor for nourishing yin, beautifying and protecting liver is developed mainly according to the requirements of women.

Clove leaves are cold in nature and bitter in taste, can protect liver, has a long history in the aspect of treating liver diseases in China, and has a good treatment effect in diseases such as acute icteric hepatitis and the like. Clove leaves contain abundant active ingredients, and Zhao Chang Wei and the like are evaluated by the change of serum transaminase (ALT, AST) activity on an APAP-induced mouse acute liver injury model. The experiment result shows that ALT and AST are obviously reduced, thereby determining that the flavone in the clove leaves has the function of protecting the liver.

Mulberry is sweet in taste and cold in nature, has the effects of promoting the production of body fluid to quench thirst, tonifying liver, tonifying kidney, nourishing yin and blood, blackening hair, sobering up, improving eyesight and soothing nerves, benefiting joints and the like, and is called folk cherry tomato. The study of Yi Wu et al on acute and chronic alcoholism of mice is carried out in the liver of the mice through mulberry polysaccharide, and the conclusion shows that the active SOD of antioxidant enzyme in the mulberry polysaccharide is increased, the consumption of GSH in liver tissues is reduced, and lipid peroxidation is reduced, so that the liver protection effect is achieved, and the mulberry polysaccharide can be used for developing the medicine for relieving alcoholism and protecting liver.

It is known from book of Zun Sheng Ba Yi (eight notes of Zun Sheng) to take 1000g of Tian Dong, 500g of Shu Di Huang, smashed Rou into powder, refined with honey into pills, and adjusted with warm wine to strengthen bone marrow, keep beauty, prolong life and lighten body weight. The Chinese date and the medlar are matched, the Chinese date is a unique fruit which is native in China, and the ancient languages are as follows: the three jujubes in one day are not aged seventy times, so that the jujubes have the effects of resisting oxidation and aging, and have certain medical effects such as the effects of benefiting heart, moistening lung, replenishing blood, beautifying, invigorating spleen and stomach, tonifying qi, promoting the production of body fluid and the like besides the medical effects of the jujubes.

The polysaccharide in the Chinese dates is also a very important active ingredient, and the Miaoming III and the like are obtained by preparing mice into an aging model of glycometabolism and comparing with pharmacological research progress of a group of high-dose and low-dose Chinese date polysaccharides, and the Chinese date polysaccharides can inhibit atrophy of immune organs and delay aging of brain tissues of the mice of the aging model, so the Chinese dates have the effects of protecting the liver, improving immunity, resisting oxidation, resisting aging and the like.

The medlar is a famous and precious medicinal material, is also a traditional medicinal material used as both medicine and food, contains rich nutrient components, has the functions of nourishing liver, improving eyesight, enriching blood, soothing nerves, tonifying kidney, replenishing vital essence, moistening lung, relieving cough, promoting the production of body fluid, quenching thirst and prolonging life, and is also always viewed by dietotherapy experts and medical experts in recent years. The lycium barbarum polysaccharide in the lycium barbarum has a certain effect, and the lycium barbarum polysaccharide is explored by the people such as the wine and the like to have the effects of immunoregulation, tumor resistance, oxidation resistance, aging resistance, blood sugar reduction, blood fat reduction, liver protection and the like.

Acanthopanax senticosus, also called Wujiashen, has a long history of eating and is a raw material of novel functional food. Acanthopanax senticosus contains rich nutrients including flavonoid, sugar, vitamin C, vitamin B2, Ca, Na, Fe, Zn, Mn and other trace elements. According to records of related Chinese medicine books, acanthopanax can effectively improve the immune function of an organism, and can relieve fatigue, regulate the central nervous system and the cardiovascular system and the like. A new health product, radix Acanthopanacis Senticosi fruit oral liquid, with tranquilizing and hypnotic effects is developed because radix Acanthopanacis Senticosi has sleep improving effect.

The invention has the following technical scheme: in the formula, the mulberry and the asparagus are monarch drugs, the mulberry nourishes yin and enriches blood, promotes fluid production, moistens intestines, the asparagus nourishes yin and moistens dryness, clears fire and promotes fluid production, and the mulberry and the asparagus are used together for tonifying but not drying. The ministerial drugs comprise prepared rehmannia root, which is used for replenishing vital essence and marrow and strengthening bone marrow, clove, which is used for warming middle energizer and lowering adverse qi, dispelling cold and relieving pain, warming kidney and tonifying yang, acanthopanax which is used for dispelling wind-damp, tonifying liver and kidney, strengthening tendons and bones and soothing nerves. Clove leaves are bitter in taste and cold in nature, have the effects of clearing heat, removing toxicity, protecting liver, benefiting gallbladder and eliminating jaundice, can relieve alcoholism, tonify liver and kidney, improve eyesight, tonify middle-jiao and Qi, harmonize spleen and stomach, and assist qi and blood in biochemical transformation, and are used as adjuvant and guiding drugs together. The medicines are combined to play the efficacies of nourishing yin and blood, warming spleen and stomach for dispelling cold and relieving alcoholism.

The invention has the following beneficial effects:

the female medicinal liquor for nourishing yin, beautifying and protecting liver, which is developed by the invention, has good taste and efficacy. The invention optimizes the proportioning and percolation process of the lady medicinal liquor formula by using an orthogonal design test. So as to obtain the optimal development method, and the female medicinal liquor can better exert the curative effect. The proportion of 4 main drugs of clove leaves, mulberry, radix asparagi and prepared rehmannia root in the formula is optimized by using sensory evaluation (from four aspects of taste, color, aroma and clarification degree), the percolation process is optimized by using the content of crude polysaccharide as an evaluation index, and 3 factors of the number of crushing meshes, the soaking time before percolation and the percolation flow rate of the medicinal materials are respectively consideredInfluence on the content of crude polysaccharide in the female medicinal liquor. The optimal mixture ratio of the components of the female medicinal liquor for nourishing yin, beautifying and protecting liver is as follows: a. the₃B₃C₁D₂Namely 10g of mulberry, 10g of radix asparagi, 5g of prepared rehmannia root and 0.4g of clove leaf, and the mulberry and the clove leaf have significance. The optimal conditions of the percolation process are as follows: a. the₂B₂C₁The grinding mesh number of the medicinal materials is selected to be 40 meshes, the soaking time before percolation is selected to be 24 hours, and the percolation flow rate is selected to be 0.8mL/min, and the percolation flow rate has significance. Under the optimized percolation process condition, the content of the crude polysaccharide is 2152.0 mg/L. The sensory evaluation and the measurement of the content of the crude polysaccharide are used as development indexes, the screening is more reasonable and scientific compared with single indexes, the operation method is simple and convenient, the result is easy to arrange, the optimized percolation process result is stable and reliable, and an important basis can be provided for further developing the medicinal liquor for women.

Drawings

FIG. 1 is a standard curve of glucose as a control of the present invention.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more clear, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. The specific embodiments described herein are merely illustrative of the invention and are not intended to be limiting.

Example 1:

a female medicated liquor for nourishing yin, caring skin and protecting liver is prepared from folium Caryophylli 0.4g, flos Caryophylli 5g, Mori fructus 10g, radix asparagi 10g, radix rehmanniae Preparata 5g, radix Acanthopanacis Senticosi 5g, fructus Lycii 5g, fructus Jujubae 10g and 1000mL of 52 ° Chinese liquor.

the method comprises the following steps: pulverizing the clove leaves, clove, mulberry, asparagus, prepared rehmannia root, acanthopanax, medlar and Chinese date in the ratio to 40 meshes respectively to obtain a medicinal material mixture for later use;

step two: dividing wine into two parts, wherein each part is 500mL for later use;

step three: putting the medicinal material mixture prepared in the first step into a container, adding a part of the wine prepared in the second step, and soaking the medicinal material mixture for 24 hours to ensure that the medicinal material mixture is fully expanded to prepare an expanded medicine;

step six: closing a piston at the outlet at the lower end of the percolation cylinder, slowly adding the other part of the wine in the step two into the top of the percolation cylinder, sealing the percolation cylinder by a cover for a period of time, and opening the piston at the outlet at the lower end of the percolation cylinder to ensure that the percolate slowly percolates at the flow rate of 0.8 ml/min;

step eight: adding diatomite which accounts for 0.05 percent of the weight of the medicinal material mixture into the semi-finished product of the medicinal liquor in a standing and precipitating period, standing and precipitating for 20 days, and filtering;

step nine: filtering the semi-finished product of the medicated wine prepared in the step eight by using filter cloth, and collecting filtrate, wherein the mesh number of the filter cloth is 120 meshes;

The formula and the preparation method of the lady medicinal liquor of the embodiment are subjected to a process optimization experiment.

1. Experimental medicinal materials and reagent

1.1 Experimental drugs

TABLE 1 Experimental drugs

1.2 Experimental reagents

TABLE 2 test reagents

1.3 Experimental facilities

TABLE 3 Experimental instruments

2. Sensory index evaluation method

The female wine for nourishing yin, beautifying and protecting liver is prepared by referring to sensory evaluation of orthogonal test design in a table 4, sensory evaluation standards comprise color, aroma, clarity and taste for tasting, 10 women aged between 20 and 40 years constitute a sensory evaluation group, and the sensory evaluation group performs sensory evaluation on wine samples of the female wine for nourishing yin, beautifying and protecting liver, which are treated differently, and an average score is obtained. See table 4 for specific scoring criteria.

TABLE 4 evaluation criteria for women's wine for nourishing yin, caring skin and protecting liver

2.1 orthogonal test of the sensory perception of lady's medicated wine

Design [ L₁₆(4⁵)]The optimal formula of the female medicinal liquor for nourishing yin, beautifying and protecting liver is found in an orthogonal test, and the factor levels are shown in a table 5. Using 4-factor-4 level [ L ]₁₆(4⁵)]Orthogonal test method, using color, aroma, taste and clarity degree as sensory index of female medicated liquor for nourishing yin, caring skin and protecting liverThe proportion of the main medicinal materials of clove leaves, mulberry, asparagus and prepared rehmannia root is determined.

TABLE 5 test factors and levels of proportioning of lady medicated liquors

3. Percolation process flow method

8 medicinal materials of clove, clove leaf, mulberry, prepared rehmannia root, cochinchnese asparagus root, acanthopanax, Chinese date and medlar are all crushed into a certain number; the 8 medicinal materials are weighed according to the accurate weight proportion and are added with 1000mL of white spirit with the alcoholic strength of 52% vol for percolation. Pulverizing the medicinal materials by a high-speed traditional Chinese medicine pulverizer, mixing and soaking the medicinal materials in 500mL of white spirit for 6 hours to enable the medicinal materials to become an expanding medicine, which is favorable for the implementation of a percolation process. Adding wetted absorbent cotton into the percolation tank, draining the liquid to the middle of the percolation tank by using a glass rod, and draining the liquid by attaching the glass rod to the wall after the absorbent cotton is immersed. Then 500mL of white spirit is added, and the mixture is sealed by a sealing film and soaked before percolation. Percolating after soaking, and collecting percolate with a clean beaker.

3.1. Percolation process optimization orthogonal experimental design

The factors influencing the development of the female medicinal liquor for nourishing yin, beautifying features and protecting liver are obtained from experience, besides sensory evaluation, the factors such as the crushing mesh number of the materials before percolation, the soaking time before percolation, the percolation flow rate and the like are also included, the variable factors are A, B, C respectively, and the three levels of the factors are adopted [ L ]₉(3⁴)]The influence of orthogonal test on the content of crude polysaccharide in the female medicinal liquor for nourishing yin, beautifying and protecting liver is researched, and the experimental factors and levels are shown in table 6.

TABLE 6 percolation process orthogonal factor level of female medicated liquor for nourishing yin, caring skin and protecting liver

Investigation on the crushing mesh number of the medicinal materials: soaking time and percolation flow rate before percolation are constant, and measuring absorbance of the medicinal materials with different pulverizing mesh numbers; the crushing mesh of the medicinal materials is 20 meshes, 40 meshes and 60 meshes respectively.

Investigation of soaking time before percolation: pulverizing the medicinal materials, determining the mesh number and percolation flow rate, and measuring the absorbance of the medicinal materials before percolation for different soaking times; the soaking time before percolation is 12h, 24h and 36h respectively.

Investigation of percolation flow rate: pulverizing the medicinal materials, soaking for a certain time before percolation, and measuring the absorbance of the medicinal materials at different percolation flow rates; the percolation flow rates were 0.8mL/min, 1.2mL/min, and 1.6mL/min, respectively.

And verifying the results of the orthogonal experiments.

3.2. Determination of crude polysaccharide content

Preparation of sample crude polysaccharide precipitate: accurately sucking 5.00mL of percolation sample solution, placing in a 50mL centrifuge tube, adding 20.00mL of anhydrous ethanol, mixing, placing in a refrigerator at 4 ℃ for standing for more than 4h, centrifuging at 4000 r/min for 10min, discarding supernatant, washing residue with 80% ethanol solution by volume fraction, centrifuging, discarding supernatant, and repeating the operation for 3 times. The remaining residue was dissolved in water and made up to 100 mL. The obtained sample is ready for use.

Drawing a glucose standard curve: accurately sucking 0.10mL, 0.20mL, 0.40mL, 0.60mL, 0.80mL and 1.00mL of glucose standard solution (2.5g/L), respectively placing the glucose standard solution, the glucose standard solution and the 1.00mL of glucose standard solution in 6 colorimetric tubes with 25mL, marking the number of the test tubes to be 1-6, respectively adding water to 2.00mL, detecting by using a phenol-sulfuric acid method, and using distilled water as a blank control group. 1.00mL of 5% phenol solution was added and 10.00mL of sulfuric acid was added, during which time it was quickly and carefully shaken well, cooled, allowed to stand at room temperature for 5min, placed in a boiling water bath for 2.00min, then cooled to room temperature and its absorbance was measured at 490 nm. Test tube 7 was used as a blank and 2.0ml of distilled water, 1ml of phenol solution, and 10ml of concentrated sulfuric acid were added.

And (3) determination of a sample: precisely measuring 0.1mL of the test solution, adding 1.9mL of water, measuring the absorbance value according to the method for measuring the glucose standard curve, and calculating the concentration of glucose in the test solution from a regression equation. The crude polysaccharide content in the sample was calculated according to the following formula.

Crude polysaccharide content (%) ═ CxDxfx100/W

C-concentration of glucose in test solution (mg/mL)

D-dilution factor of test solution

f-conversion factor

W-weight (g) of test sample

3.3. Regression equation of glucose standard curve

Drawing a glucose standard curve by taking the glucose mass concentration (X) as an abscissa and the absorbance value (Y) as an ordinate to obtain a linear regression equation of which Y is 0.0414X +0.0007 and R is²0.9956, see fig. 1 for the regression equation.

4. Component ratio sensory orthogonality test result analysis

The specific results are shown in tables 7 to 8.

TABLE 7 orthogonal table for sensory evaluation of female medicated liquor with different component ratios for nourishing yin, caring skin and protecting liver

TABLE 8 sensory evaluation analysis of variance of different ingredient ratios of female medicated liquor for nourishing yin, caring skin and protecting liver

Note: p values less than 0.05 were significant.

As can be seen from Table 7, the influence of each factor on the results of the experiment is A>D>B>C, i.e. Morous alba>Clove leaf>Asparagus cochinchinensis>Prepared rehmannia root, see table 8, mulberry and clove leaf were noted for significance in the sensory evaluation process. Finally selects A₃B₃C₁D₂The formula is the best formula, namely 10g of mulberry, 10g of radix asparagi, 5g of prepared rehmannia root and 0.4g of clove leaf, and the taste is the best.

5. Percolation process optimization orthogonal test result analysis

The results are shown in tables 9 to 11.

TABLE 9 orthogonal test result analysis of female medicated wine percolation process for nourishing yin, caring skin and protecting liver

TABLE 10 analysis of variance of percolation process of female medicated wine for nourishing yin, caring skin and protecting liver

Note: p values less than 0.05 were significant.

As can be seen from Table 9, the influence of each factor on the experimental results C > A > B, i.e., the percolation rate > the soaking time before percolation > the number of crushed meshes of the herbs.

As can be seen from table 10, the percolation flow rate was significant. Finally selects A₂B₂C₁The optimal percolation process comprises selecting the pulverizing mesh number of the medicinal materials to 40 mesh, selecting the percolation flow rate to 0.8mL/min, and selecting the soaking time before percolation to 24 h.

TABLE 11 results of the percolation process

And (4) analyzing results: as can be seen from Table 11, the same amount of the crude polysaccharide as that in the orthogonal process is adopted, the crushing mesh number of the crude polysaccharide is 40 meshes, the percolation flow rate is 0.8mL/min, the soaking time before percolation is 24h, and the parallel test is performed for 3 times, so that the crude polysaccharide content is relatively stable at 2152.0mg/L, and the orthogonal process is feasible.

6. Efficacy experiment of female medicinal liquor for nourishing yin, beautifying and protecting liver

6.1. Grouped intragastric perfusion

The clean-grade mice are randomly divided into three groups, each group comprises 8 mice, and the three groups are a blank control group, an ancient formula beauty treatment medicinal liquor gavage group and a female medicinal liquor gavage group of the embodiment. The alcohol content of the ancient prescription beauty medicated wine is the same as that of the female medicated wine in the embodiment, the wine is 52 degrees, the administration dose is the same (the dose is 13ml/kg according to the body weight of a mouse), and the blank control group is administered with the same dose of water once a day for 15 days. On the last day of experiment, each group of mice was fasted for 12h without water prohibition, weighed, bled, centrifuged (3500r/min, 10min) to prepare serum.

The ancient prescription of the beauty medicated wine is from the Chinese medical newspaper.

The ancient formula beauty medicated wine comprises the following components: 15g of ginseng (or ginseng rootlets), 15g of sharpleaf galangal fruit, 20g of whole Chinese angelica, 20g of fragrant solomonseal rhizome, 20g of cochinchnese asparagus root, 20g of dwarf lilyturf tuber, 20g of Chinese magnoliavine fruit, 30g of roasted rhizoma polygonati, 30g of prepared fleece-flower root, 30g of barbary wolfberry fruit and 30g of rehmannia root.

The application method of the preparation is as follows: the medicines are ground into coarse powder, stored in a gauze bag, soaked in 2500 ml of white spirit in a bottle, and then 250g of white sugar is added for soaking for 10 days to obtain the Chinese medicinal composition. The Chinese and evening meals are respectively taken 20-30 ml each day, and the volume of the wine can be increased or decreased appropriately. It can be taken for a long time.

The efficacy is mainly as follows: and (5) retaining youthful looks.

6.2. Detection of liver injury indicators ALT and AST

The obtained serum is used for detecting the level of alanine Aminotransferase (ALT) and aspartate Aminotransferase (AST) according to the kit operation instructions. The results are shown in Table 12.

TABLE 12 results of determination of the serum ALT and AST content changes of three groups of mice (

n＝8)

6.3. Results

Referring to AST and ALT values of a blank control group, the serum AST and ALT levels of experimental animals given the ancient formula beauty treatment medicinal liquor are found to be remarkably increased, while AST and ALT levels are slowly increased in a female medicinal liquor group given the same dose. The ALT and AST levels in serum can be increased due to liver cell damage, but the lady medicinal liquor in the embodiment is increased slowly compared with the ancient formula beauty medicinal liquor, which shows that the lady medicinal liquor in the embodiment has small liver damage.

6.4. Medicinal liquor efficacy observation experiment

Compared with experimental research, the symptom reaction of the women drinking the ancient formula beauty treatment medicinal liquor and the lady medicinal liquor of the embodiment is observed, and the observed women have the same or similar symptoms and constitutions. The two kinds of wine, 200mL, were drunk in portions, and the symptom response was observed the next day, as shown in Table 13 below.

TABLE 13 symptom reaction table of ancient prescription beauty medicated wine and lady medicated wine

6.5. The lady medicinal liquor of the embodiment is taken once a day, 50ml of the medicinal liquor is taken each time, and one month is a treatment course. The symptom reaction is compared before and after taking, the effect is obvious, and the medicine is shown in the following table 14.

TABLE 14 symptom reaction table of female medicated wine taken for a long time

In conclusion, the lady medicinal liquor has good effects of nourishing yin, beautifying features and protecting liver.

In this embodiment, the proportioning and percolation process of the lady medicinal liquor formula are optimized by using an orthogonal design test. So as to obtain the optimal development method, and the female medicinal liquor can better exert the curative effect. The proportion of 4 main drugs of clove leaves, mulberry, radix asparagi and prepared rehmannia root in the formula is optimized by sensory evaluation, the percolation process is optimized by taking the content of crude polysaccharide as an evaluation index, and the crushing mesh number, the soaking time before percolation and the percolation flow rate of the medicinal materials are respectively inspected for 3Influence of factors on the content of crude polysaccharide in the female medicinal liquor. The optimal mixture ratio of the components of the female medicinal liquor for nourishing yin, beautifying and protecting liver is as follows: a. the₃B₃C₁D₂Namely 10g of mulberry, 10g of radix asparagi, 5g of prepared rehmannia root and 0.4g of clove leaf, and the mulberry and the clove leaf have significance. The optimal conditions of the percolation process are as follows: a. the₂B₂C₁The grinding mesh number of the medicinal materials is selected to be 40 meshes, the soaking time before percolation is selected to be 24 hours, and the percolation flow rate is selected to be 0.8mL/min, and the percolation flow rate has significance. Under the optimized percolation process condition, the content of the crude polysaccharide is 2152.0 mg/L. The sensory evaluation and the measurement of the content of the crude polysaccharide are used as development indexes, the screening is more reasonable and scientific compared with single indexes, the operation method is simple and convenient, the result is easy to arrange, the optimized percolation process result is stable and reliable, and an important basis can be provided for further developing the lady medicinal liquor.

Example 2:

this example differs from example 1 only in that:

a female medicated liquor for nourishing yin, caring skin and protecting liver is prepared from folium Caryophylli 0.4g, flos Caryophylli 5g, Mori fructus 10g, radix asparagi 10g, radix rehmanniae Preparata 5g, radix Acanthopanacis Senticosi 5g, fructus Lycii 5g, fructus Jujubae 10g and 800mL of 55% (v/v) Chinese liquor.

the method comprises the following steps: taking the clove leaves, the clove, the mulberry, the asparagus, the prepared rehmannia root, the acanthopanax, the medlar and the Chinese date according to the proportion, and respectively crushing the materials into 20 meshes to obtain a medicinal material mixture for later use;

step two: dividing wine into two parts, wherein each part is 400mL for later use;

step three: putting the medicinal material mixture prepared in the step one into a container, adding a part of the wine in the step two, and soaking the medicinal material mixture for 36 hours to ensure that the medicinal material mixture is fully expanded to prepare an expanded medicine;

step six: closing a piston at the outlet at the lower end of the percolation cylinder, slowly adding the other part of the wine in the step two into the top of the percolation cylinder, sealing the percolation cylinder by a cover for a period of time, and opening the piston at the outlet at the lower end of the percolation cylinder to enable the percolate to slowly percolate at the flow rate of 1 ml/min;

step seven: collecting the percolate obtained in the sixth step, slowly adding crystal sugar after the percolate is collected, and stirring, wherein the adding amount of the crystal sugar is 1% of the weight of the medicinal material mixture, so that the crystal sugar is completely dissolved, and thus a semi-finished product of the medicinal liquor is prepared;

step eight: adding diatomite which accounts for 0.1 percent of the weight of the medicinal material mixture into the semi-finished product of the medicinal liquor in a standing and precipitating period, standing and precipitating for 30 days, and filtering;

Example 3:

this example differs from example 1 only in that:

a female medicated liquor for nourishing yin, caring skin and protecting liver is prepared from folium Caryophylli 0.8g, flos Caryophylli 10g, Mori fructus 12g, radix asparagi 12g, radix rehmanniae Preparata 10g, radix Acanthopanacis Senticosi 10g, fructus Lycii 10g, fructus Jujubae 20g and 1200mL of 55 ° Chinese liquor.

the method comprises the following steps: pulverizing the clove leaves, clove, mulberry, asparagus, prepared rehmannia root, acanthopanax, medlar and Chinese date in the ratio to 60 meshes respectively to obtain a medicinal material mixture for later use;

step two: dividing wine into two parts, wherein each part is 600mL for later use;

step three: putting the medicinal material mixture prepared in the step one into a container, adding a part of the wine in the step two, and soaking the medicinal material mixture for 30 hours to ensure that the medicinal material mixture is fully expanded to prepare an expanded medicine;

step six: closing a piston at the outlet at the lower end of the percolation cylinder, slowly adding the other part of the wine in the step two into the top of the percolation cylinder, sealing the percolation cylinder by a cover for a period of time, and opening the piston at the outlet at the lower end of the percolation cylinder to ensure that the percolate slowly percolates at the flow rate of 0.9 ml/min;

step seven: collecting the percolate obtained in the sixth step, slowly adding crystal sugar after the percolate is collected, and stirring, wherein the adding amount of the crystal sugar is 1.5% of the weight of the medicinal material mixture, so that the crystal sugar is completely dissolved, and a semi-finished product of the medicinal liquor is prepared;

step eight: removing impurities at low temperature, placing the semi-finished product in a freezing device such as refrigerator, stopping cooling when the temperature of the semi-finished product is reduced to-15 deg.C, taking out the semi-finished product, naturally increasing the temperature to 5 deg.C, and filtering.

Example 4:

this example differs from example 1 only in that: the clarifying agent is protein flocculant, amphoteric polyacrylamide flocculant and 101 fruit juice clarifying agent, and the dosage of the clarifying agent is 0.03% of the weight of the medicinal material mixture.

After the clarifying agent of the embodiment is used for replacing diatomite, the clarifying degree of the medicinal liquor for women is as follows: the lady medicinal liquor is glossy, clear and transparent, has no obvious suspended matters, has a sensory score of 13-15 minutes, and has unchanged clarity degree after being placed for a long time (at least 6 months), namely no precipitate is separated out after being placed for a long time, and the lady medicinal liquor is always clear and transformed.

Example 5:

this example differs from example 4 only in that: the amount of clarifier is 0.05% of the weight of the medicinal material mixture

The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. A preparation method of medicinal liquor for nourishing yin and beautifying features that: the composition consists of the following components: 0.2-0.4 part of clove leaf, 2.5-5 parts of clove, 5-6 parts of mulberry, 5-6 parts of radix asparagi, 2.5-5 parts of prepared rehmannia root, 2.5-5 parts of acanthopanax, 2.5-5 parts of medlar, 5-10 parts of Chinese date and 400-600 parts of wine;

the preparation method comprises the following steps:

step two: dividing wine into two parts for later use;

2. The preparation method of the yin-nourishing and beauty-maintaining female medicinal liquor according to claim 1, which is characterized in that: the composition consists of the following components: 0.2g of clove leaf, 2.5g of clove, 5g of mulberry, 5g of radix asparagi, 2.5g of prepared rehmannia root, 2.5g of acanthopanax, 2.5g of medlar, 5g of Chinese date and 500mL of wine, wherein the proportion can be increased or decreased in the same proportion.

3. The preparation method of the yin-nourishing and beauty-maintaining female medicinal liquor according to claim 1, which is characterized in that: the wine is 50-55-degree white spirit.

4. The preparation method of the yin-nourishing and beauty-maintaining female medicinal liquor according to claim 1, which is characterized in that: and step eight can be replaced by a low-temperature impurity removal method.

5. The preparation method of the yin-nourishing and beauty-maintaining female medicinal liquor according to claim 4, which is characterized in that: the low-temperature impurity removal method comprises the following steps: placing the semi-finished product of medicated liquor after standing and precipitating in a freezing device, stopping cooling when the temperature of the semi-finished product of medicated liquor is reduced to-15 deg.C, taking out the semi-finished product of medicated liquor, naturally raising the temperature to 5 deg.C, and filtering.

6. The preparation method of the yin-nourishing and beauty-maintaining female medicinal liquor according to claim 1, which is characterized in that: the clarifying agent is diatomite which is 0.05-0.1% of the weight of the medicinal material mixture.

7. The preparation method of the yin-nourishing and beauty-maintaining female medicinal liquor according to claim 1, which is characterized in that: the clarifying agent is a protein flocculating agent, an amphoteric polyacrylamide flocculating agent and a 101 fruit juice clarifying agent, and the dosage of the clarifying agent is 0.03-0.05% of the weight of the medicinal material mixture.

8. The preparation method of the yin-nourishing and beauty-maintaining female medicinal liquor according to claim 7, which is characterized in that: the ratio of the protein flocculant to the amphoteric polyacrylamide flocculant to the 101 fruit juice clarifying agent is 2:1: 2.