CN110160440B - Three-dimensional color dynamic imaging device and method based on frequency domain OCT technology - Google Patents
Three-dimensional color dynamic imaging device and method based on frequency domain OCT technology Download PDFInfo
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Abstract
The invention discloses a three-dimensional color dynamic imaging device and a method based on a frequency domain OCT technology, wherein the device comprises a spectrum optical coherence tomography system, an image cross-correlation system and a computer processing terminal; the spectral optical coherence tomography system comprises: the device comprises a first light source, a first optical fiber coupler, a first collimator, a first focusing lens, a reflecting mirror, a second light source, a second optical fiber coupler, a polarization controller, a second collimator, a two-dimensional galvanometer system, a third collimator, a grating, a second focusing lens and a CCD camera; the image cross-correlation system includes: a dichroic mirror, a third light source, and a color camera; the invention realizes three-dimensional color imaging through the spectrum optical coherence tomography scanning system and the image cross-correlation system, has high imaging precision reaching micron level, high resolution, high scanning speed and long scanning distance.
Description
Technical Field
The invention relates to the technical field of three-dimensional imaging, in particular to a three-dimensional color dynamic imaging device and method based on a frequency domain OCT technology.
Background
The existing three-dimensional imaging method mainly comprises the following steps: structured light three-dimensional imaging method and three-dimensional laser scanning technology.
The structured light three-dimensional imaging method is to carry out a large number of sequential mode projections on a sample through light rays, and then realize three-dimensional imaging by utilizing binary coding and other technologies. However, binary coding techniques have low sensitivity to object surfaces, and in order to achieve high spatial resolution, a large number of projections are required, so that objects in the scene must remain stationary during projection, extending the overall duration of three-dimensional image acquisition. Structured light three-dimensional imaging methods typically have a finite distance due to the limited energy of the light projection. In addition, this instrument is not accurate in close range measurements, and even with the highest accuracy triangulation, it can only achieve millimeter level accuracy ranging.
The three-dimensional laser scanning technology can be mainly divided into a triangle method, a pulse type and a phase type. The distance measurement by the triangulation method is shortest and the scanning speed is slow, but the distance measurement accuracy is very high, reaches the millimeter level, and is suitable for close-range precise measurement; the pulse method is longest in distance measurement and fast in scanning speed, but low in distance measurement accuracy; the phase ranging method has higher ranging precision, is suitable for medium-distance measurement, but has less application in the laser scanning technology.
Although the above methods can realize three-dimensional imaging, the respective defects exist. Although the structured light three-dimensional imaging technology has good imaging effect, the structured light three-dimensional imaging technology is time-consuming, the precision only reaches the millimeter level, and the scanning distance is limited. Although the three-dimensional laser scanning technology has long scanning distance, the accuracy, the distance measurement and the scanning speed have contradictory relation, the higher the accuracy is, the slower the scanning speed is, and the highest accuracy can only reach millimeter level.
Disclosure of Invention
Aiming at the problems existing in the prior art, the invention provides a three-dimensional color dynamic imaging device based on the frequency domain OCT technology, which has high resolution, high scanning speed, long scanning distance and color imaging;
on the other hand, a three-dimensional color dynamic imaging method based on a frequency domain OCT technology for realizing dynamic imaging is provided.
The invention solves the technical problems as follows: a three-dimensional color dynamic imaging device based on a frequency domain OCT technology comprises a spectrum optical coherence tomography system, an image cross-correlation system and a computer processing terminal;
The spectral optical coherence tomography system comprises: the device comprises a first light source, a first optical fiber coupler, a first collimator, a first focusing lens, a reflecting mirror, a second light source, a second optical fiber coupler, a polarization controller, a second collimator, a two-dimensional galvanometer system, a third collimator, a grating, a second focusing lens and a CCD camera;
The image cross-correlation system includes: a dichroic mirror, a third light source, and a color camera;
the first collimator is connected with the reflector through a first focusing lens, the emergent light of the second collimator enters the two-dimensional galvanometer system to deflect, and the deflected emergent light is transmitted to the sample through the dichroic mirror;
The third light source is in light connection with the dichroic mirror through a reflecting surface of the spectroscope, the spectroscope reflects emergent light of the third light source at an incident angle of 45 degrees, the reflected light of the spectroscope is reflected by the dichroic mirror and then is emitted to the sample, and the color camera is used for receiving transmitted light of the spectroscope;
The emergent light of the third collimator is focused in the second focusing lens after being split by the grating, and the focused emergent light is received by the CCD camera;
The first optical fiber coupler is connected with the first light source, the first collimator, the third collimator and the second optical fiber coupler through optical fibers respectively, the second optical fiber coupler is connected with one end of the second light source and one end of the polarization controller through optical fibers respectively, the other end of the polarization controller is connected with the second collimator through light rays, and the computer processing terminal is electrically connected with the CCD camera, the color camera and the two-dimensional galvanometer system respectively.
As a further improvement of the technical scheme, the CCD camera is a linear array CCD camera.
As a further improvement of the technical scheme, the spectroscope has a spectroscope ratio of 50:50.
As a further improvement of the above technical solution, the first light source and the second light source are both laser light sources.
A three-dimensional color dynamic imaging method based on a frequency domain OCT technique, the method comprising:
Obtaining an OCT image of a sample to obtain a three-dimensional stereogram of the sample;
Obtaining a two-dimensional color image of a sample, and obtaining two-dimensional color information of the sample;
Registering the two-dimensional color signals to the three-dimensional stereogram through a normalized cross-correlation matching algorithm to obtain a three-dimensional color imaging chart.
As a further improvement of the above technical solution, the process for obtaining a three-dimensional stereogram of a sample includes:
And obtaining an OCT image of the sample according to an optical coherence tomography principle, and reconstructing a three-dimensional stereogram of the sample according to depth information of different positions of the surface of the sample in the OCT image.
The beneficial effects of the invention are as follows: on one hand, the device realizes three-dimensional color imaging through the spectral optical coherence tomography scanning system and the image cross-correlation system, has high imaging precision reaching the micron level, high resolution, high scanning speed and long scanning distance.
On the other hand, the invention registers the acquired two-dimensional color information to the three-dimensional stereogram of the acquired and calculated motion sample through a normalized cross-correlation matching algorithm, and performs scanning correction according to the calculated sample offset in the scanning process, thereby realizing dynamic three-dimensional color imaging.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings that are required to be used in the description of the embodiments will be briefly described below. It is evident that the drawings described are only some embodiments of the invention, but not all embodiments, and that other designs and drawings can be obtained from these drawings by a person skilled in the art without inventive effort.
FIG. 1 is a schematic view of the structure of the device of the present invention;
FIG. 2 is a flow chart of the present invention;
FIG. 3 is a top view of a three-dimensional perspective of a sample;
FIG. 4 is a top view of a two-dimensional color signal of a sample;
FIG. 5 is a three-dimensional topographical view of a sample;
fig. 6 is a three-dimensional color imaging diagram of a sample.
Detailed Description
The conception, specific structure, and technical effects produced by the present invention will be clearly and completely described below with reference to the embodiments and the drawings to fully understand the objects, features, and effects of the present invention. It is apparent that the described embodiments are only some embodiments of the present invention, but not all embodiments, and that other embodiments obtained by those skilled in the art without inventive effort are within the scope of the present invention based on the embodiments of the present invention. In addition, all connection relationships mentioned herein do not refer to direct connection of the components, but rather, refer to a connection structure that may be better formed by adding or subtracting connection aids depending on the particular implementation. The technical features in the invention can be interactively combined on the premise of no contradiction and conflict.
Embodiment 1, referring to fig. 1, a three-dimensional color dynamic imaging device based on the frequency domain OCT technology includes a spectral optical coherence tomography system, an image cross-correlation system, and a computer processing terminal 122;
the spectral optical coherence tomography system comprises: a first light source 101, a first fiber coupler 102, a first collimator 103, a first focusing lens 104, a reflecting mirror 105, a second light source 106, a second fiber coupler 107, a polarization controller 108, a second collimator 109, a two-dimensional galvanometer system 110, a third collimator 115, a grating 116, a second focusing lens 117, and a CCD camera 118;
The image cross-correlation system includes: a dichroic mirror 111, a dichroic mirror 112, a third light source 113, and a color camera 114;
The first collimator 103 is connected with the reflecting mirror 105 through the first focusing lens 104, the emergent light of the second collimator 109 enters the two-dimensional galvanometer system 110 to deflect, and the deflected emergent light is transmitted through the dichroic mirror 111 to the sample 120;
The third light source 113 is connected with the dichroic mirror 111 through a reflecting surface of the dichroic mirror 112, the dichroic mirror 112 reflects the emergent light of the third light source 113 at an incident angle of 45 degrees, the reflected light of the dichroic mirror 112 is reflected by the dichroic mirror 111 and then is directed to the sample 120, and the color camera 114 is used for receiving the transmitted light of the dichroic mirror 112;
The outgoing light of the third collimator 115 is split by the grating 116 and then focused in the second focusing lens 117, and the focused outgoing light is received by the CCD camera 118;
The first optical fiber coupler 102 is connected with the first light source 101, the first collimator 103, the third collimator 115 and the second optical fiber coupler 107 through optical fibers, the second optical fiber coupler 107 is connected with one end of the second light source 106 and one end of the polarization controller 108 through optical fibers, the other end of the polarization controller 108 is connected with the second collimator 109 through light rays, and the computer processing terminal 122 is electrically connected with the CCD camera 118, the color camera 114 and the two-dimensional galvanometer system 110.
OCT as described herein is an optical coherence tomography technique, and the two-dimensional galvanometer system 110 includes an X-axis mirror and a Y-axis mirror. The polarization controller 108 is used to change the polarization direction of the transmitted light in the fiber.
Preferably, the CCD camera 118 is a linear CCD camera. The linear array CCD camera performs a line scan of the sample 120. The grating 116 is used for obtaining a linear optical signal after light splitting, and all optical signals can be rapidly collected by using a linear array CCD camera, so that the collection speed is high.
Preferably, the splitting ratio of the beam splitter 112 is 50:50.
The beam splitter 112 is used to form a coaxial light source that illuminates a color camera 114.
As an optimization, the first light source 101 and the second light source 106 are both laser light sources.
The first light source 101 and the second light source 106 are laser emitters, the center wavelength of the first light source 101 is 840nm, the full width at half maximum is 50nm, and the center wavelength of the second light source 106 is 671nm. The third light source 113 is a white light source.
As an optimization, an assemblable lens group 119 is further disposed between the dichroic mirror 111 and the sample 120, the outgoing light of the dichroic mirror 111 is transmitted through the assemblable lens group 119 and then directed to the sample 120, and the assemblable lens group 119 can automatically adjust parameters such as focal length and field of view of the lens, so as to be beneficial to better focusing on the sample 120.
The spectral optical coherence tomography system is used for collecting depth information of the surface of the sample 120, and a three-dimensional stereogram of the sample 120 is calculated through the computer processing terminal 122. The image cross-correlation system is used for acquiring a two-dimensional color image of the sample 120, and two-dimensional color information is obtained through the computer processing terminal 122.
The focused spot diameter of the first light source 101 is about 9.02 μm, and in air, the lateral resolution is 9.02 μm and the axial resolution is 7.78 μm. The imaging precision of the three-dimensional color dynamic image formed by the three-dimensional color dynamic imaging device based on the frequency domain OCT technology reaches the micron level, and the three-dimensional color dynamic imaging device has the characteristic of high resolution.
Meanwhile, the first light source 101 and the second light source 106 are multi-wavelength laser light sources, and can perform remote three-dimensional color imaging.
The device realizes three-dimensional color imaging through the spectrum optical coherence tomography scanning system and the image cross-correlation system, has high imaging precision reaching the micron level, high resolution, high scanning speed and long scanning distance.
The three-dimensional color dynamic imaging device based on the frequency domain OCT technology comprises a three-dimensional color dynamic imaging method based on the frequency domain OCT technology, wherein the three-dimensional color dynamic imaging method based on the frequency domain OCT technology is applied to the three-dimensional color dynamic imaging device based on the frequency domain OCT technology, and the method comprises the following steps:
obtaining an OCT image of the sample 120, and obtaining a three-dimensional perspective view of the sample 120;
Obtaining a two-dimensional color image of the sample 120, and obtaining two-dimensional color information of the sample 120;
Registering the two-dimensional color signals to the three-dimensional stereogram through a normalized cross-correlation matching algorithm to obtain a three-dimensional color imaging chart.
As an optimization, the process of obtaining the three-dimensional perspective view of the sample 120 includes:
According to the OCT principle, OCT image of the sample 120 is obtained, and then according to the depth information of different positions of the surface of the sample 120 in the OCT image, a three-dimensional stereogram of the sample 120 is reconstructed.
Referring to fig. 2, the working principle of the present invention:
The light beam emitted by the first light source 101 enters the first optical fiber coupler 102 and is divided into a first light beam and a second light beam according to a 50:50 light splitting ratio, the first light beam enters the first collimator 103, and the second light beam enters the second optical fiber coupler 107;
the first light beam is collimated and parallel by the first collimator 103, and is focused to the reflecting mirror 105 through the first focusing lens 104, the reflecting mirror 105 reflects the focused first light beam, and the reflected first light beam returns to the first optical fiber coupler 102 along the original path;
The second light beam and the light beam emitted by the second light source 106 are emitted into the second optical fiber coupler 107 to be combined into a beam of low-coherence light, the low-coherence light passes through the polarization controller 108 and then is emitted into the second collimator 109 to be collimated and parallel, the collimated and parallel low-coherence light enters the two-dimensional vibrating mirror system 110 to deflect, the deflected low-coherence light passes through the dichroic mirror 111 and is emitted into the sample 120, the low-coherence light is scattered on the sample 120 to obtain back scattered light, and the back scattered light returns to the first optical fiber coupler 102 along an original path;
After the low-coherence light is scattered for multiple times on the surface of the sample 120, back scattered light carrying depth information on the surface of the sample 120 is obtained;
The first light beam returned to the first optical fiber coupler 102 interferes with the back scattered light to generate interference light, the interference light enters a third collimator 115 to be collimated and parallel, the emergent light of the third collimator 115 is focused in a second focusing lens 117 after being split by a grating 116, the focused emergent light is received by a CCD camera 118, the CCD camera 118 converts a received optical signal into an electric signal and transmits the electric signal to the computer processing terminal 122, and the computer processing terminal 122 processes the received electric signal to obtain depth information of a sample 120;
The first light beam returning to the first fiber coupler 102 interferes with the backscattered light to generate interference light, i.e. a michelson interferometer. As known from the monochromatic light interference theory, the interference light intensity of the first fiber coupler 102 can be expressed as:
Wherein I R is the DC signal of the first light beam, I S is the DC signal of the back scattered light, A R is the amplitude of the first light beam, A S is the amplitude of the back scattered light, z j is the detection depth of the aplanatic plane, Is a phase difference. When the interference light is split by the grating 116, the intensity signal received by each linear array unit of the CCD camera 118 can be expressed as:
The interference light intensity signal received by the linear array CCD camera is transformed from the wave vector space to the coordinate space through Fourier transformation, and in ideal cases, the signal is expressed as:
where z is the optical path distance of the mirror 105 to the first collimator 103, z i is the optical path distance of the sample 120 to the dichroic mirror 111, δ is a delta function, and the interference intensity is maximum if and only if z-z i =0. Thus, depth information of the sample 120 can be obtained from the optical path difference z-z i.
Referring to fig. 3 and 5, the computer processing terminal 122 is electrically connected to the two-dimensional galvanometer system 110. The computer processing terminal 122 provides control voltage for the two-dimensional galvanometer system 110, changes the deflection angles of the X-axis mirror and the Y-axis mirror by changing the voltages transmitted to the X-axis mirror and the Y-axis mirror in the two-dimensional galvanometer system 110 respectively, so that the sample 120 is periodically scanned by low coherence light, complete and continuous OCT images of the sample 120 are obtained according to the optical coherence tomography technology, and a three-dimensional stereogram of the sample 120 is reconstructed according to depth information of different positions of the surface of the sample 120 in the OCT images.
Meanwhile, referring to fig. 4, the image cross-correlation system may acquire two-dimensional color images of the sample 120 while it is in motion in real time. The light beam emitted by the third light source 113 is refracted by the beam splitter 112 with an incident angle of 45 ° and reaches the dichroic mirror 111, the reflected light of the beam splitter 112 is reflected by the dichroic mirror 111 and is applied to the sample 120, the light beam is reflected on the surface of the sample 120, the reflected light returns to the dichroic mirror 111 along the original path, the reflected light is reflected by the dichroic mirror 111 and is transmitted to the color camera 114 through the beam splitter 112, so as to obtain a two-dimensional color image of the sample 120, and the color camera 114 converts the received light signal into an electrical signal and transmits the electrical signal to the computer processing terminal 122.
The computer processing terminal 122 performs a time-domain cross-correlation operation on two adjacent two-dimensional color images to obtain an offset of the motion of the sample 120, where a mathematical expression of the offset D (t) is:
Where a (t), b (t) represent two-dimensional color images of two adjacent frames, t is an iteration index, i is an index pointing to the first element of the current frame, and W is an image length.
In the scanning process, the dynamic sample 120 may shift, so as to realize complete collection of the dynamic sample 120, the image cross-correlation system converts the shift into a correction control voltage value of the two-dimensional galvanometer system 110, and transmits the correction control voltage value to the two-dimensional galvanometer system 110, and the positions of the scanning in the sample 120 after the sample 120 is moved are positioned by changing the voltages of the X-axis mirror and the Y-axis mirror which are transmitted to the two-dimensional galvanometer system 110, and changing the deflection angles of the X-axis mirror and the Y-axis mirror, so that each line scanning of the spectral optical coherence tomography system is corrected by the shift of the movement of the sample 120.
Thus, the spectral optical coherence tomography system can track the sample 120 in the scanning motion, and the sample 120 is subjected to periodic line scanning and simultaneously is subjected to scanning correction according to the offset of the motion of the sample 120, so that the complete OCT image of the sample 120 is obtained, dynamic imaging is realized, and the misplaced data information is avoided.
After the periodic scanning is completed, a complete and continuous OCT image of the sample 120 is obtained according to the optical coherence tomography principle, and a three-dimensional stereogram of the sample 120 is reconstructed according to depth information of different positions of the surface of the sample 120 in the OCT image. Registering a two-dimensional color signal obtained according to a two-dimensional color image on a three-dimensional stereogram through a normalized cross-correlation matching algorithm, wherein the operation process comprises the following steps:
Let the pixel size of the two-dimensional color image I to be matched be mxn and the pixel size of the template T be mxn. And arbitrarily selecting a sub-picture I x,y with the pixel size of M multiplied by N from the two-dimensional color image I, wherein the coordinates of the upper left corner of the sub-picture I in the two-dimensional color image I are (x, y), and the coordinate range is 0-x-M, 0-y-N. Wherein, M, N are the number of rows and columns of the two-dimensional color image I pixels to be matched, and M, N are the number of rows and columns of the template pixels, respectively.
The normalized cross-correlation value R (x, y) of sub-graph I x,y and template T is defined as
Wherein (i, j) is the coordinates of the pixel in the template T;
Is the pixel average value of sub-graph I x,y;
Is the pixel average of the template T.
All normalized cross-correlation values constitute a normalized cross-correlation matrix R.
Referring to fig. 6, a normalized cross-correlation value R (x, y) is calculated through normalized cross-correlation to obtain an offset required for registering a two-dimensional color signal to a three-dimensional stereogram, rotation translation is performed on the two-dimensional color information to match the three-dimensional stereogram, and fusion processing is performed on the rotation translated two-dimensional color information and the three-dimensional stereogram to obtain a three-dimensional color imaging diagram. Dynamic color three-dimensional imaging is realized through continuous multiple acquisition.
According to the invention, the acquired two-dimensional color information is registered to the three-dimensional stereogram of the acquired and calculated moving sample 120 through a normalized cross-correlation matching algorithm, and scanning correction is carried out according to the calculated sample 120 offset in the scanning process, so that dynamic three-dimensional color imaging is realized, the imaging precision reaches the micron level, the resolution is high, and the scanning distance is large.
While the preferred embodiment of the present application has been described in detail, the application is not limited to the embodiments, and various equivalent modifications and substitutions can be made by those skilled in the art without departing from the spirit of the application, and these modifications and substitutions are intended to be included in the scope of the present application as defined in the appended claims.
Claims (5)
1. A three-dimensional color dynamic imaging method based on a frequency domain OCT technology is characterized in that: the device comprises a spectrum optical coherence tomography system, an image cross-correlation system and a computer processing terminal; the spectral optical coherence tomography system comprises: the device comprises a first light source, a first optical fiber coupler, a first collimator, a first focusing lens, a reflecting mirror, a second light source, a second optical fiber coupler, a polarization controller, a second collimator, a two-dimensional galvanometer system, a third collimator, a grating, a second focusing lens and a CCD camera; the image cross-correlation system includes: a dichroic mirror, a third light source, and a color camera; the first optical fiber coupler is connected with the first light source, the first collimator, the third collimator and the second optical fiber coupler through optical fibers respectively, the second optical fiber coupler is connected with one end of the second light source and one end of the polarization controller through optical fibers respectively, the other end of the polarization controller is connected with the second collimator through light rays, and the computer processing terminal is electrically connected with the CCD camera, the color camera and the two-dimensional galvanometer system respectively; the first collimator is connected with the reflector through a first focusing lens, the emergent light of the second collimator enters the two-dimensional galvanometer system to deflect, and the deflected emergent light is transmitted to the sample through the dichroic mirror; the third light source is in light connection with the dichroic mirror through a reflecting surface of the spectroscope, the spectroscope reflects emergent light of the third light source at an incident angle of 45 degrees, the reflected light of the spectroscope is reflected by the dichroic mirror and then is emitted to the sample, and the color camera is used for receiving the transmitted light of the spectroscope, so that a two-dimensional color image of the sample is obtained; the emergent light of the third collimator is focused in a second focusing lens after being split by the grating, and the focused emergent light is received by a CCD camera, so that an OCT image of the sample is obtained;
The method comprises the following steps:
Obtaining an OCT image of a sample to obtain a three-dimensional stereogram of the sample;
Obtaining a two-dimensional color image of a sample, and obtaining two-dimensional color information of the sample;
Registering the two-dimensional color signals to the three-dimensional stereogram through a normalized cross-correlation matching algorithm to obtain a three-dimensional color imaging chart;
Wherein the step of registering the two-dimensional color signals to the three-dimensional stereogram by a normalized cross-correlation matching algorithm comprises:
Let the pixel size of the two-dimensional color image I to be matched be The pixel size of the template T is/>Arbitrarily selecting a block of pixels from the two-dimensional color image I as/>Subgraph/>The upper left corner thereof has a coordinate/>, in the two-dimensional color image ICoordinate range is/>,/>,/>Respectively the number of rows and columns of the pixels of the two-dimensional color image I to be matched,/>The number of rows and columns, respectively, of template pixels, then subgraph/>Normalized cross-correlation value with template T/>The definition is as follows:
;
Wherein, Coordinates of pixels in the template T; /(I)For subgraph/>Is a pixel average value of (1); /(I)Is the pixel average value of the template T; all normalized cross-correlation values form a normalized cross-correlation matrix R;
Calculating normalized cross-correlation value by normalized cross-correlation Obtaining the offset required by registering the two-dimensional color signals to the three-dimensional stereogram, carrying out rotary translation on the two-dimensional color information to match the three-dimensional stereogram, carrying out fusion processing on the rotary translated two-dimensional color information and the three-dimensional stereogram to obtain a three-dimensional color imaging diagram, and realizing dynamic color three-dimensional imaging through continuous multiple acquisition;
In the scanning process of the spectrum optical coherence tomography system, the computer processing terminal carries out time domain cross correlation operation on two adjacent frames of the two-dimensional color images to obtain the offset of the sample motion, the image cross correlation system converts the offset into a correction control voltage value of the two-dimensional galvanometer system and transmits the correction control voltage value to the two-dimensional galvanometer system, and the deflection angles of an X-axis reflector and a Y-axis reflector in the two-dimensional galvanometer system are changed by changing the voltage of the X-axis reflector and the Y-axis reflector, so that each line scanning of the spectrum optical coherence tomography system is corrected through the offset of the sample motion.
2. The method for three-dimensional color dynamic imaging based on the frequency domain OCT technique according to claim 1, wherein: the CCD camera is a linear array CCD camera.
3. The method for three-dimensional color dynamic imaging based on the frequency domain OCT technique according to claim 1, wherein: the spectroscope has a 50:50 spectroscope ratio.
4. The method for three-dimensional color dynamic imaging based on the frequency domain OCT technique according to claim 1, wherein: the first light source and the second light source are both laser light sources.
5. The method for three-dimensional color dynamic imaging based on the frequency domain OCT technique according to claim 1, wherein: the process for obtaining the three-dimensional stereogram of the sample comprises the following steps:
And obtaining an OCT image of the sample according to an optical coherence tomography principle, and reconstructing a three-dimensional stereogram of the sample according to depth information of different positions of the surface of the sample in the OCT image.
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