CN110152062A - A kind of 3D biometric print hydrogel scaffold and preparation method thereof applied to regeneration - Google Patents
A kind of 3D biometric print hydrogel scaffold and preparation method thereof applied to regeneration Download PDFInfo
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- CN110152062A CN110152062A CN201910426708.6A CN201910426708A CN110152062A CN 110152062 A CN110152062 A CN 110152062A CN 201910426708 A CN201910426708 A CN 201910426708A CN 110152062 A CN110152062 A CN 110152062A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/26—Mixtures of macromolecular compounds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/52—Hydrogels or hydrocolloids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C64/00—Additive manufacturing, i.e. manufacturing of three-dimensional [3D] objects by additive deposition, additive agglomeration or additive layering, e.g. by 3D printing, stereolithography or selective laser sintering
- B29C64/10—Processes of additive manufacturing
- B29C64/106—Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C64/00—Additive manufacturing, i.e. manufacturing of three-dimensional [3D] objects by additive deposition, additive agglomeration or additive layering, e.g. by 3D printing, stereolithography or selective laser sintering
- B29C64/20—Apparatus for additive manufacturing; Details thereof or accessories therefor
- B29C64/205—Means for applying layers
- B29C64/209—Heads; Nozzles
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C64/00—Additive manufacturing, i.e. manufacturing of three-dimensional [3D] objects by additive deposition, additive agglomeration or additive layering, e.g. by 3D printing, stereolithography or selective laser sintering
- B29C64/30—Auxiliary operations or equipment
- B29C64/386—Data acquisition or data processing for additive manufacturing
- B29C64/393—Data acquisition or data processing for additive manufacturing for controlling or regulating additive manufacturing processes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y10/00—Processes of additive manufacturing
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y30/00—Apparatus for additive manufacturing; Details thereof or accessories therefor
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y50/00—Data acquisition or data processing for additive manufacturing
- B33Y50/02—Data acquisition or data processing for additive manufacturing for controlling or regulating additive manufacturing processes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/32—Materials or treatment for tissue regeneration for nerve reconstruction
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Abstract
The present invention is directed primarily to a kind of printable 3D hydrogel scaffold and preparation method thereof, belongs to field of tissue engineering technology.It is provided by the invention can 3D printing hydrogel scaffold by having biocompatibility and the gellan gum of shear thinning behavioral trait and the soluble starch mixing of plasticity and can print by 3D printing technique that surface is smooth and the compound rest of the complete hollow out hexagonal prisms structure of tool, to be used for tissue damage reparation and nerve regneration.The bracket is with good stability, and is printed by biomaterial that is degradable, absorbing.The bionical internal cell three-dimensional growing space of the support height conducive to the discharge of the attachment of cell and the diffusion of permission nutriment and waste cell can be sufficiently grown and is proliferated.
Description
Technical field
The present invention relates to field of tissue engineering technology, are applied to regeneration 3D biometric print hydrogel particularly with regard to one kind
Bracket and preparation method thereof particularly belongs to tissue engineering nerve regeneration field.
Background technique
Peripheral nerve is the bridge for contacting nerve center and periphery target structure, and major function is impression stimulation, will be neural
Impulsion afferent nerve maincenter, and neural impulsion is spread out of, dominate muscular movement and glandular secretion.Peripheral nerve defection is
Peripheral nerve caused by referring to wound or performing the operation does detachment, after restoring its " bioelastic " under functional location, between the broken ends of fractured bone still
There are spacing.In daily production and life, traffic accident, industrial injury, war, earthquake and clinical operation surprisingly etc. caused by around
Neurotrosis is common clinical sign.According to the statistics of the World Health Organization, the annual kainogenesis peripheral nerve injury case about 36 in the U.S.
Ten thousand, the annual new cases in Europe are more than 300,000.China's peripheral nerve injury case increases about 1,000,000 newly every year, wherein week
Case about 300,000 for enclosing neurologic defect.
Clinically peripheral nerve defect is still using traditional autologous nerve as Nerve grafting at present, in recent years,
Neurologic defect is repaired using tissue engineering nerve bridge material and obtains remarkable progress, and many products are own to be entered clinical or will enter
Clinical use.
However traditional autologous nerve bridge material limited source, tissue size and structure mismatches and graft is for the head of district
The reasons such as phase denervation, secondary damage, therefore the application of nerve autograft has significant limitation.Common artificial neuron
The repairing effect of graft is not also highly desirable compared with autotransplantation, especially repair over long distances, coarse or outmoded nerve lacks
When damage, there are also very big gaps compared with autotransplantation for repairing effect.
Therefore develop it is a kind of it is novel be comparable nerve autograft object, guidance nerve grows into distal side and new life nerve
The 3D biometric print hydrogel scaffold that can be used for nerve regneration of plastotype effect has wide face as artificial nerve graft
Bed application prospect.
Summary of the invention
The technical problem to be solved in the present invention is that providing the 3D biometric print hydrogel for being used for nerve regneration load cells
Bracket and preparation method thereof.For print carriage provided by the invention after 3D printing, gelation is good.
In order to solve the above technical problems, the technical solution adopted by the present invention is that:
The 3D biometric print hydrogel scaffold and preparation method thereof that the present invention provides a kind of applied to regeneration, the water
Gel stent by have biocompatibility and can the gellan gum of shear thinning behavioral trait and the soluble starch of plastic properties mix
Merge by 3D printing technique prepare surface it is smooth and have complete structure compound rest.
The present invention provides a kind of preparation methods of 3D biometric print hydrogel scaffold applied to regeneration, specific to walk
It is rapid as follows:
Step 1: gellan gum powder being added in water, prepares gellan gum solution；
Step 2: based on the basis of step 1, weighing soluble starch powder, be configured to gellan gum and starch mixing is molten
Liquid；
Step 3: the mixed solution of step 2 pouring into 50ml centrifuge tube, places cooling at room temperature；
Step 4: gellan gum and starch mixed solution after will be cooled be put into 4 DEG C of refrigerator overnights, obtain being used to prepare 3D biology
Print the raw material of hydrogel scaffold.
Step 5: cooling mixed raw material being put into the 3D printer barrel based on extrusion, according to the model having been introduced into
It is printed, obtains compound rest.
Step 6: printed bracket is carried out shaping into sterilization treatment.
Step 7: processing being modified to the bracket to have sterilized, achievees the purpose that be modified cradling function, then can connect
Kind cell.
In one embodiment of the present invention, the water is ultrapure water.
In one embodiment of the present invention, gellan gum (GG) is added in ultrapure water in the step 1, stirring in water bath is molten
Solution, is made gellan gum aqueous solution.
In one embodiment of the present invention, the mass ratio of ultrapure water and gellan gum is 70-100 in the step 1:
16。
In one embodiment of the present invention, the step 2 is to be added centainly in the resulting gellan gum aqueous solution of step 1
The soluble starch powder of ratio, is prepared into gellan gum and soluble starch mixed solution.
In one embodiment of the present invention, the mass ratio of ultrapure water, gellan gum and soluble starch in the step 2
For 70 ~ 100:16:4 ~ 16.
In one embodiment of the present invention, in the step 2, the bath temperature for preparing mixed solution is 90 ~ 100 DEG C.
In one embodiment of the present invention, in the step 2, prepares mixed solution and need to stir 30 ~ 40min.
In one embodiment of the present invention, in the step 2, preparing mixed solution to need mixing speed is 1000rpm.
In one embodiment of the present invention, in the step 3, the mixed solution prepared needs cold at room temperature
It after general 2h, is put into 50ml centrifuge tube and continues cooling 1h.
In one embodiment of the present invention, in the step 5, the 3D printing model of importing is the cuboid of different side lengths
Or square, the print speed of 3D printer are 15 ~ 20mm/s, 240 DEG C of nozzle temperature, 17 DEG C of platform temperature, print syringe needle
Diameter is 0.74mm, prints thickness 1mm, the filling distance 2mm, and air pressure is 0.11 ~ 0.3MPa.
In one embodiment of the present invention, printed bracket is put into -20 DEG C of refrigerator freezing 48h in the step 6,
36h in -50 DEG C of vacuum freeze drier, last ultraviolet sterilization 2h are put it into again.
In one embodiment of the present invention, modification processing method employed in the step 7 is the branch that will have been sterilized
The YIGSR polypeptide of bridge joint branch various concentration (0.5-10 μ g/ml), grafting time 1h.
Compared with prior art, one kind of the invention has for 3D biometric print hydrogel scaffold and preparation method thereof
Below the utility model has the advantages that
1, the three dimensional growth environment of hydrogel scaffold analog cell of the invention can promote cell conducive to the attachment of cell
Growth and proliferation；
2, the present invention in, have biocompatibility, nontoxicity and can shear thinning behavior gellan gum can with plasticity can
The compound rest of dissolubility gellan gum meets the requirement of structural transplantation, can be applied to nerve regneration.
Detailed description of the invention
Fig. 1 is that the 3D biometric print hydrogel scaffold of regeneration in the present invention prepares schematic diagram；
Fig. 2 is the 3D biometric print hydrogel scaffold schematic diagram of the resulting regeneration of embodiment 1 in the present invention；
Fig. 3 is the 3D biometric print hydrogel scaffold schematic diagram of the resulting regeneration of embodiment 2 in the present invention.
Specific embodiment
Below with reference to embodiment to further detailed description of the present invention, embodiments of the present invention are not limited thereto.
Embodiment 1:
Compound rest 3D printing material is made by the raw material of following mass parts:
16 parts of gellan gum
4 parts of soluble starch
80 parts of water
In present example, water is ultrapure water, can avoid the damage of ion pair bracket.
In present example, gellan gum is food-grade, heat-resisting, acid resistance is good, also high to the stability of enzyme.Heating is
It is dissolved into transparent solution, after cooling, forms transparent and solid gel.Suitable for melting extrusion 3D printing.
In present example, starch is soluble starch, does not dissolve in cold water, is dissolved in boiling water, forms gel after cooling.
The ratio of soluble starch and gellan gum is 4:1.Under this ratio, printing material can smoothly be extruded into rope,
It is smooth and have the compound rest of complete structure that surface is piled into according to printing path.
Specific step is as follows:
Step 1: the gellan gum powder of 16g being added in ultrapure water, prepares gellan gum solution；
Step 2: based on the basis of step 1, weighing soluble starch powder 4g, be configured to gellan gum and starch mixing is molten
Liquid, wherein temperature is 90 DEG C, mixing speed 1000rpm/min, mixing time 40min, if weather is colder, can be stirred
1h；
Step 3: the mixed solution of step 2 pouring into 50ml centrifuge tube, places cooling at room temperature；
Step 4: gellan gum and starch mixed solution after will be cooled be put into 4 DEG C of refrigerator overnights, obtain being used to prepare 3D biology
Print the raw material of hydrogel scaffold；
Step 5: cooling mixed raw material being put into the 3D printer barrel based on extrusion, is carried out according to the model having been introduced into
Printing, the 3D printing model of importing are 1.5cm × 1.5cm × 0.3cm cuboid, and the print speed of 3D printer is 15mm/
S, 240 DEG C of nozzle temperature, 17 DEG C of platform temperature, printing needle diameter is 0.74mm, prints thickness 1mm, the filling distance 2mm,
Air pressure is 0.11MPa, obtains compound rest；
Step 6: printed bracket being put into -20 DEG C and is carried out shaping, then sterilization treatment；
Step 7: processing being modified to the bracket to have sterilized, achievees the purpose that be modified cradling function, then can be inoculated with thin
Born of the same parents.
Embodiment 2:
Compound rest 3D printing material is made by the raw material of following mass parts:
16 parts of gellan gum
5.33 parts of soluble starch
8.67 parts of water
In present example, starch is soluble starch, does not dissolve in cold water, is dissolved in boiling water, forms gel after cooling.It is solvable
Property starch and gellan gum ratio be 3:1.Under this ratio, printing material can smoothly be extruded into rope, according to
It is smooth and have the compound rest of complete structure that printing path is piled into surface.
In present example, the 3D printing material reasonable mixture ratio of components provided, gelation rate is fast in print procedure, can be fast
Rapid-result type, the supporting structure printed are complete.
The preparation method and subsequent processing of 3D printing material of the present invention include:
Step 1: the gellan gum powder of 16g being added in ultrapure water, prepares gellan gum solution；
Step 2: based on the basis of step 1, weighing soluble starch powder 5.33g, be configured to gellan gum and starch mixes
Solution is closed, wherein temperature is 90 DEG C, mixing speed 1000rpm/min, mixing time 40min, if weather is colder, can be stirred
Mix 1h；
Step 3: the mixed solution of step 2 pouring into 50ml centrifuge tube, places cooling at room temperature；
Step 4: gellan gum and starch mixed solution after will be cooled be put into 4 DEG C of refrigerator overnights, obtain being used to prepare 3D biology
Print the raw material of hydrogel scaffold.
Step 5: cooling mixed raw material being put into the 3D printer barrel based on extrusion, according to the model having been introduced into
It is printed, the 3D printing model of importing is 1.5cm × 1.5cm × 0.3cm cuboid, and the print speed of 3D printer is
15mm/s, 240 DEG C of nozzle temperature, 17 DEG C of platform temperature, printing needle diameter is 0.74mm, prints thickness 1mm, and the filling distance is
2mm, air pressure 0.11MPa, obtains compound rest.
Step 6: printed bracket being put into -20 DEG C and is carried out shaping, then sterilization treatment.
Step 7: processing being modified to the bracket to have sterilized, achievees the purpose that be modified cradling function, then can connect
Kind cell.
Embodiment 3:
Compound rest 3D printing material is made by the raw material of following mass parts:
16 parts of gellan gum
8 parts of soluble starch
76 parts of water
In present example, starch is soluble starch, does not dissolve in cold water, is dissolved in boiling water, forms gel after cooling.It is solvable
Property starch and gellan gum ratio be 2:1.Under this ratio, printing material can smoothly be extruded into rope, according to
It is smooth and have the compound rest of complete structure that printing path is piled into surface.
In present example, the 3D printing material reasonable mixture ratio of components provided, gelation rate is fast in print procedure, can be fast
Rapid-result type, the supporting structure printed are complete.
The preparation method and subsequent processing of 3D printing material of the present invention include:
Step 1: the gellan gum powder of 16g being added in ultrapure water, prepares gellan gum solution；
Step 2: based on the basis of step 1, weighing soluble starch powder 8g, be configured to gellan gum and starch mixing is molten
Liquid, wherein temperature is 90 DEG C, mixing speed 1000rpm/min, mixing time 40min, if weather is colder, can be stirred
1h；
Step 3: the mixed solution of step 2 pouring into 50ml centrifuge tube, places cooling at room temperature；
Step 4: gellan gum and starch mixed solution after will be cooled be put into 4 DEG C of refrigerator overnights, obtain being used to prepare 3D biology
Print the raw material of hydrogel scaffold.
Step 5: cooling mixed raw material being put into the 3D printer barrel based on extrusion, according to the model having been introduced into
It is printed, the 3D printing model of importing is 2cm × 2cm × 0.5cm cuboid, and the print speed of 3D printer is 20mm/
S, 240 DEG C of nozzle temperature, 17 DEG C of platform temperature, printing needle diameter is 1mm, prints thickness 1mm, the filling distance 2mm, air pressure
For 0.2MPa, compound rest is obtained.
Step 6: printed bracket being put into -20 DEG C and is carried out shaping, then sterilization treatment.
Step 7: processing being modified to the bracket to have sterilized, achievees the purpose that be modified cradling function, then can connect
Kind cell.
Embodiment 4:
Compound rest 3D printing material is made by the raw material of following mass parts:
16 parts of gellan gum
16 parts of soluble starch
70 parts of water
In present example, starch is soluble starch, does not dissolve in cold water, is dissolved in boiling water, forms gel after cooling.It is solvable
Property starch and gellan gum ratio be 1:1.Under this ratio, printing material can smoothly be extruded into rope, according to
It is smooth and have the compound rest of complete structure that printing path is piled into surface.
In present example, the 3D printing material reasonable mixture ratio of components provided, gelation rate is fast in print procedure, can be fast
Rapid-result type, the supporting structure printed are complete.
The preparation method and subsequent processing of 3D printing material of the present invention include:
Step 1: the gellan gum powder of 16g being added in ultrapure water, prepares gellan gum solution；
Step 2: based on the basis of step 1, weighing soluble starch powder 16g, be configured to gellan gum and starch mixing
Solution, wherein temperature is 90 DEG C, mixing speed 1000rpm/min, mixing time 40min, if weather is colder, can be stirred
1h；
Step 3: the mixed solution of step 2 pouring into 50ml centrifuge tube, places cooling at room temperature；
Step 4: gellan gum and starch mixed solution after will be cooled be put into 4 DEG C of refrigerator overnights, obtain being used to prepare 3D biology
Print the raw material of hydrogel scaffold.
Step 5: cooling mixed raw material being put into the 3D printer barrel based on extrusion, according to the model having been introduced into
It is printed, the 3D printing model of importing is 2cm × 2cm × 0.5cm cuboid, and the print speed of 3D printer is 20mm/
S, 240 DEG C of nozzle temperature, 17 DEG C of platform temperature, printing needle diameter is 1mm, prints thickness 1mm, the filling distance 2mm, air pressure
For 0.3MPa, compound rest is obtained.
Step 6: printed bracket being put into -20 DEG C and is carried out shaping, then sterilization treatment.
Step 7: processing being modified to the bracket to have sterilized, achievees the purpose that be modified cradling function, then can connect
Kind cell.
The present invention relates to field of tissue engineering technology, a kind of gellan gum that can be applied to regeneration 3D printing and solubility are provided
The preparation method of the compound rest of starch.Printing material is to mix gellan gum and starch according to different proportion, prints table
Face is smooth and has the compound rest of complete structure.Specifically in conjunction with gellan gum biocompatibility and can shear thinning behavioral trait
With the plasticity feature of starch, the bracket of composite tissue engineering regeneration requirements is prepared.Analogue body inner cell three dimensional growth space allows thin
Born of the same parents can sufficiently grow and be proliferated.
Claims (10)
1. a kind of 3 biometric print hydrogel scaffolds applied to regeneration, it is characterised in that: described applied to regeneration
3D biometric print hydrogel scaffold is combined by gellan gum and soluble starch.
2. a kind of preparation method of 3D biometric print hydrogel scaffold applied to regeneration according to claim 1,
It is characterized in that comprising the steps of:
Step 1: gellan gum powder being added in ultrapure water, prepares gellan gum solution；
Step 2: based on the basis of step 1, weigh soluble starch powder, be added into gellan gum solution be configured to tie it is cold
Glue and starch mixed solution；
Step 3: the mixed solution of step 2 pouring into 50ml centrifuge tube, places cooling at room temperature；
Step 4: gellan gum and starch mixed solution after will be cooled be put into 4 DEG C of refrigerator overnights, obtain being used to prepare 3D biology
Print the raw material of hydrogel scaffold；
Step 5: cooling mixed raw material being put into the 3D printer barrel based on extrusion, is carried out according to the model having been introduced into
Printing is to get compound rest；
Step 6: printed bracket is carried out shaping into sterilization treatment；
Step 7: processing being modified to the bracket to have sterilized, achievees the purpose that be modified cradling function, then can be inoculated with thin
Born of the same parents.
3. a kind of preparation method of 3D biometric print hydrogel scaffold applied to regeneration according to claim 2,
It is characterized by: the mass ratio of ultrapure water and gellan gum is 70-100:16 in the step 1.
4. a kind of preparation method of 3D biometric print hydrogel scaffold applied to regeneration according to claim 2,
It is characterized by: the mass ratio of ultrapure water, gellan gum and soluble starch is 70 ~ 100:16:4 ~ 16 in the step 2；System
The bath temperature of standby gellan gum and starch mixed solution is 90 ~ 100 DEG C；Wherein, gellan gum and starch mixed solution needs are prepared
Stir 30 ~ 40min.
5. a kind of preparation method of 3D biometric print hydrogel scaffold applied to regeneration according to claim 2,
It is characterized by: prepare gellan gum in the step 2 and starch mixed solution to need mixing speed be 1000rpm.
6. a kind of preparation method of 3D biometric print hydrogel scaffold applied to regeneration according to claim 2,
It is characterized by: after the gellan gum and starch mixed solution that prepare in the step 3 need to cool down 2h at room temperature, then
It is put into 50ml centrifuge tube and continues cooling 1h.
7. a kind of preparation method of 3D biometric print hydrogel scaffold applied to regeneration according to claim 2,
It is characterized by: the 3D printing model imported in the step 5 is the cuboid or square of different side lengths, used mould
Type Software for producing is Solidworks.
8. a kind of preparation method of 3D biometric print hydrogel scaffold applied to regeneration according to claim 2,
It is characterized by: in the step 5 3D printer setting: print speed be 15 ~ 20mm/s, 240 DEG C of nozzle temperature, platform temperature
17 DEG C of degree, printing needle diameter are 0.74mm, print thickness 1mm, the filling distance 2mm, and air pressure is 0.11 ~ 0.3MPa.
9. a kind of preparation method of 3D biometric print hydrogel scaffold applied to regeneration according to claim 2,
It is characterized by: printed bracket is put into -20 DEG C of refrigerator freezing 48h in the step 6, then put it into -50 DEG C true
36h in vacuum freecing-dry machine, last ultraviolet sterilization 2h.
10. a kind of preparation method of 3D biometric print hydrogel scaffold applied to regeneration according to claim 2,
It is characterized by: modification processing method employed in the step 7 is the bracket physical absorption various concentration that will have been sterilized
The YIGSR polypeptide of (0.5-10 μ g/ml), grafting time 1h.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110917401A (en) * | 2019-11-01 | 2020-03-27 | 海口市人民医院 | 3D printing support for bile duct repair and preparation method thereof |
| CN113372626A (en) * | 2021-04-25 | 2021-09-10 | 中北大学 | Composite functional material for direct 3D printing and printing method |
| CN114128884A (en) * | 2020-09-03 | 2022-03-04 | 江苏省农业科学院 | 3D printing Chinese yam starch loaded carotenoid sustained release preparation and preparation method thereof |
| CN114196043A (en) * | 2021-11-25 | 2022-03-18 | 华南理工大学 | High-water-absorptivity starch hydrogel based on 3D printing technology and preparation method and application thereof |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101658692A (en) * | 2008-08-27 | 2010-03-03 | 山东省生物药物研究院 | Gellan gum composition, preparation method thereof and application thereof |
| WO2010132028A1 (en) * | 2009-05-15 | 2010-11-18 | Nanyang Technological University | Composition for manufacturing a scaffold for tissue engineering, and a method of making it |
| CN104004231A (en) * | 2014-06-12 | 2014-08-27 | 东南大学 | Biomacromolecule interpenetrating polymer network hydrogel and preparation method thereof |
| CN105233344A (en) * | 2015-10-22 | 2016-01-13 | 温州医科大学 | Composite repair material for bridging defect nerves and stent made of composite repair material |
| EP3131412A1 (en) * | 2014-02-03 | 2017-02-22 | Nederlandse Organisatie voor toegepast- natuurwetenschappelijk onderzoek TNO | Method for the production of an edible object by powder bed (3d) printing and food products obtainable therewith |
| CN107573660A (en) * | 2017-09-14 | 2018-01-12 | 北京化工大学 | A kind of low temperature FDM types biological medical degradable 3D printing material, preparation and application |
| CN107668306A (en) * | 2017-11-22 | 2018-02-09 | 无限极（中国）有限公司 | Gel candy 3d printing material and preparation method thereof |
| CN108402264A (en) * | 2018-02-09 | 2018-08-17 | 华中科技大学 | A kind of optional gel soft candy 3D printing material and preparation method thereof for carrying complex polysaccharide |
-
2019
- 2019-05-22 CN CN201910426708.6A patent/CN110152062A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101658692A (en) * | 2008-08-27 | 2010-03-03 | 山东省生物药物研究院 | Gellan gum composition, preparation method thereof and application thereof |
| WO2010132028A1 (en) * | 2009-05-15 | 2010-11-18 | Nanyang Technological University | Composition for manufacturing a scaffold for tissue engineering, and a method of making it |
| EP3131412A1 (en) * | 2014-02-03 | 2017-02-22 | Nederlandse Organisatie voor toegepast- natuurwetenschappelijk onderzoek TNO | Method for the production of an edible object by powder bed (3d) printing and food products obtainable therewith |
| CN104004231A (en) * | 2014-06-12 | 2014-08-27 | 东南大学 | Biomacromolecule interpenetrating polymer network hydrogel and preparation method thereof |
| CN105233344A (en) * | 2015-10-22 | 2016-01-13 | 温州医科大学 | Composite repair material for bridging defect nerves and stent made of composite repair material |
| CN107573660A (en) * | 2017-09-14 | 2018-01-12 | 北京化工大学 | A kind of low temperature FDM types biological medical degradable 3D printing material, preparation and application |
| CN107668306A (en) * | 2017-11-22 | 2018-02-09 | 无限极（中国）有限公司 | Gel candy 3d printing material and preparation method thereof |
| CN108402264A (en) * | 2018-02-09 | 2018-08-17 | 华中科技大学 | A kind of optional gel soft candy 3D printing material and preparation method thereof for carrying complex polysaccharide |
Non-Patent Citations (3)
| Title |
|---|
| CAMERON J.FERRIS等: "Modified gellan gum hydrogels for tissue engineering applications", 《SOFT MATTER》 * |
| ILHAN YU等: "A study on degradation behavior of 3D printed gellan gum scaffolds", 《PROCIDIA CIRP》 * |
| 曾戎，屠美: "《生物医用仿生高分子材料》", 30 October 2010, 广州：华南理工大学出版社 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110917401A (en) * | 2019-11-01 | 2020-03-27 | 海口市人民医院 | 3D printing support for bile duct repair and preparation method thereof |
| CN114128884A (en) * | 2020-09-03 | 2022-03-04 | 江苏省农业科学院 | 3D printing Chinese yam starch loaded carotenoid sustained release preparation and preparation method thereof |
| CN113372626A (en) * | 2021-04-25 | 2021-09-10 | 中北大学 | Composite functional material for direct 3D printing and printing method |
| CN113372626B (en) * | 2021-04-25 | 2022-09-13 | 中北大学 | Composite functional material for direct 3D printing and printing method |
| CN114196043A (en) * | 2021-11-25 | 2022-03-18 | 华南理工大学 | High-water-absorptivity starch hydrogel based on 3D printing technology and preparation method and application thereof |
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