CN110093343A - A kind of integrated portable nucleic acid-extracting apparatus and method for extracting nucleic acid - Google Patents
A kind of integrated portable nucleic acid-extracting apparatus and method for extracting nucleic acid Download PDFInfo
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- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 79
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 78
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 78
- 238000000034 method Methods 0.000 title claims abstract description 17
- 238000012360 testing method Methods 0.000 claims abstract description 95
- 238000013519 translation Methods 0.000 claims abstract description 4
- 238000010438 heat treatment Methods 0.000 claims description 27
- 230000005291 magnetic effect Effects 0.000 claims description 24
- 239000011324 bead Substances 0.000 claims description 18
- 238000004659 sterilization and disinfection Methods 0.000 claims description 8
- 238000009826 distribution Methods 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 238000012546 transfer Methods 0.000 claims description 4
- 230000009514 concussion Effects 0.000 claims description 3
- 238000010828 elution Methods 0.000 claims description 3
- 238000000338 in vitro Methods 0.000 claims description 3
- 238000000605 extraction Methods 0.000 abstract description 19
- 238000004140 cleaning Methods 0.000 description 12
- 230000000694 effects Effects 0.000 description 11
- 238000002474 experimental method Methods 0.000 description 8
- 238000005336 cracking Methods 0.000 description 6
- 230000008901 benefit Effects 0.000 description 5
- 238000012864 cross contamination Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 230000007613 environmental effect Effects 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000003776 cleavage reaction Methods 0.000 description 3
- 230000007017 scission Effects 0.000 description 3
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 230000005298 paramagnetic effect Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000009666 routine test Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- TVEXGJYMHHTVKP-UHFFFAOYSA-N 6-oxabicyclo[3.2.1]oct-3-en-7-one Chemical compound C1C2C(=O)OC1C=CC2 TVEXGJYMHHTVKP-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
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- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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Abstract
The present invention provides a kind of integrated portable nucleic acid-extracting apparatus, including shell and the controller, bar magnet, bar magnet set, the test tube platform that are built in shell；Bar magnet, bar magnet set and test tube platform are set gradually from top to bottom, and bar magnet passes vertically through bar magnet set, can be placed in the test tube of test tube platform；Bar magnet, bar magnet set can oscilaltion, and translation can be controlled；Bar magnet set can shake；Opening is arranged in the front side wall of shell, and test tube platform can be moved forward and backward, and passes in and out shell from opening；Controller is electrically connected with bar magnet, bar magnet set respectively.The tool for extracting nucleic acid is built in unified shell by integrated portable nucleic acid-extracting apparatus of the invention, is carried convenient for user and is carried out nucleic acid extraction at any time.On this basis, the invention also discloses a kind of method for extracting nucleic acid.
Description
Technical field
The present invention relates to bioscience research and applied technical fields, in particular to a kind of integrated portable nucleic acid
Extraction element and method for extracting nucleic acid.
Background technique
Instrument for extracting nucleic acid is the instrument for being automatically performed sample nucleic acid using matched nucleic acid extracting reagent and extracting work.Extensively
It is general to apply in Center for Disease Control, clinical disease diagnosis, transfusion safety, Forensic Identification, environmental microorganism detection, food safety
The multiple fields such as detection, animal husbandry and molecular biology research.
Method for extracting nucleic acid generally comprises centrifugal column method and paramagnetic particle method.The extract equipment of centrifugal column method is expensive, efficiency
Lowly, paramagnetic particle method extract equipment is due to having the advantages that the mainstream instrument high-efficient, at low cost for already becoming nucleic acid extraction in the market
Device.
It can be divided according to instrument model is of different sizes are as follows: large-scale automated fluid work station and small-sized automatic nucleic acid extraction
Instrument.Wherein, large-scale automated fluid work station is the very powerful complete set of equipments of function, builds and maintenance requires bigger money
Gold, extracting nucleic acid is an application in its numerous function, and unsuitable Routine Test Lab is used to extract nucleic acid.And it is small-sized from
Dynamic instrument for extracting nucleic acid is to achieve the purpose that automatically extract nucleic acid by the particularity of operating structure, can be obtained in any laboratory
To application.
Even however, small-sized automatic instrument for extracting nucleic acid, although there is automation, high throughput, safety and environmental protection, operate just
Victory can handle the advantages that different type sample simultaneously, and there are still extracting tool integrated levels, and not high, sample of nucleic acid taking-up is put into not side
Just the deficiencies of.
So how to improve small-sized instrument for extracting nucleic acid structure design, improve equipment integrated level facilitate equipment carry and
Using, to improve nucleic acid extraction speed and effect be those skilled in the art's urgent problem to be solved.
Summary of the invention
The purpose of the present invention is to provide a kind of integrated portable nucleic acid-extracting apparatus and method for extracting nucleic acid, existing to solve
There is, sample of nucleic acid taking-up not high using existing extracting tool integrated level when small-sized instrument for extracting nucleic acid in technology to be put into inconvenience etc.
Problem.
To achieve the goals above, the invention adopts the following technical scheme:
A kind of integrated portable nucleic acid-extracting apparatus provided by the invention including shell and is built in the control of the shell
Device, bar magnet, bar magnet set and test tube platform；The bar magnet, bar magnet set and the test tube platform are set gradually from top to bottom, institute
It states bar magnet and passes vertically through the bar magnet set, can be placed in the test tube of the test tube platform；The bar magnet, bar magnet set are equal
Can oscilaltion, and translation can be controlled；The bar magnet set can shake；Opening, institute is arranged in the front side wall of the shell
Stating test tube platform can be moved forward and backward, and pass in and out the shell from the opening；The controller respectively with the bar magnet, the magnetic
Stick set electrical connection.
Based on the above technical solution, further, the outer of the bar magnet set is provided with multiple annular projections, multiple
The circumferentially extending that the annular projection is covered along the bar magnet, the outer profile of the annular projection are in round and smooth shape.
The rounded profile for having the technical effect that annular projection of the technical solution can be avoided the lesser magnetic bead of size and stay
There are the outer walls of bar magnet set.
Based on the above technical solution, further, the quantity of the bar magnet be it is multiple, multiple bar magnets one by one before
After be distributed；The quantity of the bar magnet set is multiple, multiple bar magnet set front and back distributions one by one；It is provided on the test tube platform
Multiple rows of test tube, test tube described in each row are arranged in left-right direction, and multiple rows of test tube is sequentially arranged along the longitudinal direction.
The technical solution has the technical effect that multiple bar magnets, multiple bar magnets set and multiple rows of test tube are correspondingly arranged, same
Same or different sample of nucleic acid can be extracted in batch simultaneously, improves the efficiency of nucleic acid extraction, is facilitated and is compared and count.
It further include the heating device being electrically connected with the controller further on the basis of any of the above-described technical solution；
The heating device is set to the bottom on the test tube platform sinistral and/or right side, for carrying out to invisible spectro sample of nucleic acid
Heating.
The technical solution has the technical effect that nucleic acid cleavage needs to carry out in the case where being higher than environment temperature, utilizes controller
Test tube needs temperature to be achieved when accurately can quickly control experiment.Additionally, due to only needing to the test tube for cracking and eluting
Heating, therefore the setting heating device in test tube platform two sides.Wherein, the upper surface of heating device is provided with multiple vallecular cavities, is used for
Holding test tubes.On the one hand vallecular cavity can provide cavity for test tube, stablize test tube in the extraction process such as vibration and place, another party
The stickiness of face vallecular cavity is good, conducive to stationary temperature environment is formed.
On the basis of any of the above-described technical solution, further, the heating device is integrated with the test tube platform to be set
It sets, alternatively, the heating device, the test tube platform are detachably arranged with the test tube.
The heating device for having the technical effect that integrated setting and test tube platform stabilization of the technical solution are preferable, benefit
In user's quiet run.Dismountable heating device, test tube platform and test tube using flexible are higher, can be according to practical need
Suitable test tube is selected to be operated, also, when test tube platform or test tube damage wherein, it can only replacement be wherein
One component reduces equipment use cost.
It further include observation window further on the basis of any of the above-described technical solution；The observation window is arranged in the shell
On the front side wall of body.
The technical solution has the technical effect that the front of shell is arranged in observation window, observes nucleic acid conducive to user and mentions
Take process.Distinguishingly, the glass of observation window is preferably arranged to dark brown or taupe, to reduce radiation of the ultraviolet light to the external world,
Observation window preferably uses acrylic or analog material to make, and avoids the disadvantage that glass material is frangible.
It further include control panel further on the basis of any of the above-described technical solution；The control panel is arranged in institute
The upper surface for stating shell is electrically connected with the controller.
The technical solution has the technical effect that control panel is used to carry out the operation for having step to nucleic acid extraction process,
It such as selects the test tube quantity of cracking, select the intracorporal environment temperature of shell in the quantity of cleaning test tube, and control experiment.Into
One step, can set control panel to and housing hinge, and operator is facilitated to be tested according to practical matter.
The present invention also provides a kind of method for extracting nucleic acid based on integrated portable nucleic acid-extracting apparatus, including walk as follows
It is rapid:
Step 1: being in vitro put into sample of nucleic acid and magnetic bead to be extracted, starting heating device is heated；
Step 2: being stirred concussion to sample of nucleic acid using bar magnet set；
Step 3: to another test tube and eluting nucleic acid using bar magnet transfer magnetic bead.
Based on the above technical solution, further, after step 3 terminates to take out sample of nucleic acid, sterilamp is opened
It carries out disinfection sterilization to enclosure interior.
The technical solution has the technical effect that before sample of nucleic acid cracking, sterilamp can irradiate extraction process generation
Free nucleic acid, avoid front and back batch between pollution.
On the basis of any of the above-described technical solution, further, in step 3, first at least one before elution nucleic acid
Magnetic bead is cleaned in test tube.
The technical solution has the technical effect that existing more reagent strip cleanings magnetic bead mechanism, is that first addition cleaning solution is right
Magnetic bead is adsorbed on to magnet hole one side of cleaning afterwards, is then siphoned away unbonded substance with cleaning needle, it is repeatedly more in this way
Secondary, magnetic bead is easy to be sucked away, cross contamination and does not wash clean clearly.At least one cleaning test tube is arranged in the present apparatus, to avoid magnetic
Pearl cross contamination, and can thoroughly clean.
The invention has the following beneficial effects:
The tool for extracting nucleic acid is built in unified shell by integrated portable nucleic acid-extracting apparatus provided by the invention,
And test tube platform can steadily pass in and out shell, carry convenient for user and carry out nucleic acid extraction at any time.
Additional technical feature and its advantage of the invention will illustrate more obvious in following description content, or pass through
Concrete practice of the invention is recognized that.
Detailed description of the invention
In order to illustrate more clearly of the technical solution of the specific embodiment of the invention, specific embodiment will be described below
Needed in attached drawing be briefly described.It should be evident that the accompanying drawings in the following description is some implementations of the invention
Mode for those of ordinary skill in the art without creative efforts, can also be according to these attached drawings
Obtain other attached drawings.
Fig. 1 is the perspective view of integrated portable nucleic acid-extracting apparatus provided in an embodiment of the present invention；
Fig. 2 is the schematic diagram of internal structure of integrated portable nucleic acid-extracting apparatus provided in an embodiment of the present invention；
Fig. 3 is the schematic diagram of internal structure at visual angle from front to back in Fig. 2；
Fig. 4 is the outline drawing of bar magnet set in integrated portable nucleic acid-extracting apparatus provided in an embodiment of the present invention；
Fig. 5 is the top view of test tube platform in integrated portable nucleic acid-extracting apparatus provided in an embodiment of the present invention.
Icon: 1- shell；2- bar magnet；3- bar magnet set；4- test tube platform；5- opening；6- heating device；7- vallecular cavity；8- is seen
Examine window；9- control panel；10- annular projection；11- sliding rail.
Specific embodiment
Technical solution of the present invention is clearly and completely described below in conjunction with attached drawing, it is clear that described implementation
Example is a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, ordinary skill
Personnel's every other embodiment obtained without making creative work, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that term " center ", "upper", "lower", "left", "right", "vertical",
The orientation or positional relationship of the instructions such as "horizontal", "inner", "outside" be based on the orientation or positional relationship shown in the drawings, merely to
Convenient for description the present invention and simplify description, rather than the device or element of indication or suggestion meaning must have a particular orientation,
It is constructed and operated in a specific orientation, therefore is not considered as limiting the invention.In addition, term " first ", " second ",
" third " is used for descriptive purposes only and cannot be understood as indicating or suggesting relative importance.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " installation ", " phase
Even ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or be integrally connected；It can
To be mechanical connection, it is also possible to be electrically connected；It can be directly connected, can also can be indirectly connected through an intermediary
Connection inside two elements.For the ordinary skill in the art, above-mentioned term can be understood at this with concrete condition
Concrete meaning in invention.
One, DESCRIPTION OF THE PRIOR ART:
In the prior art, instrument for extracting nucleic acid can be divided according to instrument model is of different sizes are as follows: large-scale automated fluid work
It stands and small-sized automatic instrument for extracting nucleic acid.Wherein, large-scale automated fluid work station is the very powerful complete set of equipments of function, build and
Maintenance requires bigger fund, and extracting nucleic acid is an application in its numerous function, unsuitable Routine Test Lab
For extracting nucleic acid.And small-sized automatic instrument for extracting nucleic acid is to reach the mesh for automatically extracting nucleic acid by the particularity of operating structure
, it can be applied in any laboratory.
Even however, small-sized automatic instrument for extracting nucleic acid, although there is automation, high throughput, safety and environmental protection, operate just
Victory can handle the advantages that different type sample simultaneously, and there are still extracting tool integrated levels, and not high, sample of nucleic acid taking-up is put into not side
Just, nucleic acid cleavage and the deficiencies of low extraction effect.
Two, technical solution of the present invention is summarized:
Integrated portable nucleic acid-extracting apparatus provided by the invention including shell 1 and is built in the controller of shell 1, magnetic
Stick 2, bar magnet set 3 and test tube platform 4；Bar magnet 2, bar magnet set 3 and test tube platform 4 are set gradually from top to bottom, and bar magnet 2 passes vertically through
Bar magnet set 3, can be placed in the test tube of test tube platform 4；Bar magnet 2, bar magnet set 3 can oscilaltion, and can control flat
It moves；Bar magnet set 3 can shake；The front side wall setting opening 5 of shell 1, test tube platform 4 can be moved forward and backward, and be passed in and out from opening 5
Shell 1；Controller is electrically connected with bar magnet 2, bar magnet set 3 respectively.
The technical solution of above-mentioned integrated portable nucleic acid-extracting apparatus can be solved preferably in the prior art using small-sized
Inconvenient, operation panel dependence entirety that existing extracting tool integrated level is not high when instrument for extracting nucleic acid, sample of nucleic acid taking-up is put into
It is computer, inconvenient to carry and the problems such as operate whenever and wherever possible: the tool for extracting nucleic acid being built in unified shell 1, and is tried
Pipe platform 4 can steadily pass in and out shell 1, carry convenient for user and carry out nucleic acid extraction at any time.
Three, technical solution of the present invention specific embodiment:
For above-mentioned prior art, below with reference to specific embodiment to skill of the invention
Art scheme is further explained explanation:
Present embodiments provide a kind of integrated portable nucleic acid-extracting apparatus, in which: Fig. 1 is provided in an embodiment of the present invention
The perspective view of integrated portable nucleic acid-extracting apparatus；Fig. 2 is integrated portable nucleic acid-extracting apparatus provided in an embodiment of the present invention
Schematic diagram of internal structure；Fig. 3 is the schematic diagram of internal structure at visual angle from front to back in Fig. 2；Fig. 4 provides for the embodiment of the present invention
Integrated portable nucleic acid-extracting apparatus in test tube platform 4 top view.As shown in Figure 1 to 4, integrated portable nucleic acid extraction
Device includes shell 1 and is built in the controller of shell 1, bar magnet 2, bar magnet set 3 and test tube platform 4；Bar magnet 2, bar magnet set 3 and examination
Pipe platform 4 is set gradually from top to bottom, and bar magnet 2 passes vertically through bar magnet set 3, can be placed in the test tube of test tube platform 4；Bar magnet
2, bar magnet set 3 can oscilaltion, and translation can be controlled；Bar magnet set 3 can shake；The front side wall setting of shell 1 is opened
Mouth 5, test tube platform 4 can be moved forward and backward, and pass in and out shell 1 from opening 5；Controller is electrically connected with bar magnet 2, bar magnet set 3 respectively.Magnetic
The outer of stick set 3 is provided with multiple annular projections 10, circumferentially extending of multiple annular projections 10 along bar magnet set 3.Wherein, bar magnet 2,
Bar magnet set 3 is respectively arranged at movable support, and movable support is arranged on shell 1, has oscilaltion and all around translates
Three freedom of movement.The movement of the movable support can be driven by rack-and-pinion, can also be driven by stepper motor.And
And the movable support where bar magnet set 3 is provided with oscillator, is able to drive bar magnet set 3 and horizontal direction and/or vertical direction occurs
Frequency vibration.
Wherein, sample of nucleic acid when annular projection 10 is conducive to bar magnet 3 oscillation of set cracks, and improves nucleic acid extraction rate and effect
Fruit.
In addition, disposable for convenience shift all test tubes of present lot on test tube platform 4, and rear is completed in experiment
Just nucleic acid transfer is efficiently carried out, sliding rail 11, direct pull can be set between the lower part of test tube platform 4 and the inside of shell 1
The transfer of entire test tube platform 4 can be realized in opening 5.
On the basis of the above embodiments, as shown in figure 3, further, the outer profile of annular projection 10 is in round and smooth shape, energy
The lesser magnetic bead of size is enough avoided to be deposited in the outer wall of bar magnet set 3.
On the basis of the above embodiments, as shown in Fig. 2, Fig. 5, further, the quantity of bar magnet 2 is multiple, multiple magnetic
The front and back distribution one by one of stick 2；The quantity of bar magnet set 3 is multiple, multiple front and back distributions one by one of bar magnet set 3；It is provided on test tube platform 4
Multiple rows of test tube, each row's test tube are arranged in left-right direction, and multiple rows of test tube is sequentially arranged along the longitudinal direction.In this configuration, multiple
Bar magnet 2, multiple bar magnets set 3 and multiple rows of test tube are correspondingly arranged, and can extract same or different nucleic acid simultaneously in same batch
Sample improves the efficiency of nucleic acid extraction, facilitates and compares and count.
It on the basis of the above embodiments, further include being electrically connected with the controller further as shown in Fig. 2, Fig. 3, Fig. 5
Heating device 6；Heating device 6 is set to the bottom on 4 left side of test tube platform or right side, for invisible spectro sample of nucleic acid
It is heated.Since nucleic acid cleavage needs to carry out in the case where being higher than environment temperature, experiment accurately can be quickly controlled using controller
When test tube need temperature to be achieved.Additionally, due to only needing to the test tube heating for cracking and eluting, therefore at 4 liang of test tube platform
The setting heating device 6 of side.Wherein, the upper surface of heating device 6 is provided with multiple vallecular cavities 7, is used for holding test tubes.At this point, slot
7 one side of chamber can provide cavity for test tube, stablize test tube in the extraction process such as vibration and place, another aspect vallecular cavity 7
Stickiness is good, conducive to stationary temperature environment is formed.
In addition, heating device 6 may be configured as with 4 integrated setting of test tube platform, alternatively, heating device 6, test tube platform 4
Detachably it is arranged with test tube.The heating device 6 of integrated setting and 4 stability of test tube platform are preferable, steadily grasp conducive to user
Make.Dismountable heating device 6, test tube platform 4 and test tube using flexible are higher, and it is suitable to select according to actual needs
Test tube is operated, also, when test tube platform 4 or test tube damage wherein, can only replace one of component, is dropped
Low equipment use cost.
On the basis of the above embodiments, as shown in Figure 1, further including observation window 8 further；Observation window 8 is arranged in shell
On the front side wall of body 1.In this configuration, the front of shell 1 is arranged in observation window 8, is conducive to user and observes nucleic acid extraction mistake
Journey.Distinguishingly, the glass of observation window 8 is preferably arranged to dark brown or taupe, to reduce ultraviolet light to extraneous radiation, observation
Window 8 is preferably made of acrylic or analog material, avoids the disadvantage that glass material is frangible.
On the basis of the above embodiments, as shown in Figure 1, further including control panel 9 further；Control panel 9 is arranged
In the upper surface of shell 1, it is electrically connected with the controller.In this configuration, control panel 9 is for having carried out step to nucleic acid extraction process
Rapid operation such as selects the test tube quantity of cracking, selects in the quantity of cleaning test tube, and control experiment in shell 1
Environment temperature.Further, control panel 9 can be set to hinged with shell 1, operator is facilitated to be carried out according to practical matter
Experiment.
It should be noted that being additionally provided with sterilamp in shell 1, it is electrically connected with the controller.In this configuration, sterilamp is made
With disinfection and sterilization is, it can be controlled in front of experiment and is irradiated after experiment, reduce influence of the environmental factor to experimental result.
The present invention also provides a kind of method for extracting nucleic acid based on integrated portable nucleic acid-extracting apparatus, including walk as follows
It is rapid:
Step 1: being in vitro put into sample of nucleic acid and magnetic bead to be extracted, starting heating device 6 is heated；
Step 2: covering 3 pairs of sample of nucleic acid using bar magnet is stirred concussion；
Step 3: shifting magnetic bead using bar magnet 2 to another test tube and elutes nucleic acid.
On the basis of the above embodiments, further, terminate in step 3, after taking out sample of nucleic acid, open sterilizing
Lamp is to the sterilization that carries out disinfection inside shell 1.Before sample of nucleic acid cracking, sterilamp can irradiate the free core of extraction process generation
Acid avoids the pollution between front and back batch.
On the basis of the above embodiments, further, in step 3, first at least one test tube before elution nucleic acid
In magnetic bead is cleaned.Existing more reagent strips clean magnetic bead mechanism, are that cleaning solution is first added then to be inhaled magnetic bead with magnet
Unbonded substance on one side, is then siphoned away with cleaning needle, wants repeated multiple times in this way by the hole for being attached to cleaning, and magnetic bead is easy to be inhaled
It walks, cross contamination and wash clean clearly.At least one cleaning test tube is arranged in the present apparatus, to avoid magnetic bead cross contamination, and energy
Enough thoroughly cleanings.
Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention., rather than its limitations；To the greatest extent
Pipe present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that: its according to
So be possible to modify the technical solutions described in the foregoing embodiments, or to some or all of the technical features into
Row equivalent replacement；And these are modified or replaceed, various embodiments of the present invention technology that it does not separate the essence of the corresponding technical solution
The range of scheme.
In addition, it will be appreciated by those of skill in the art that although above-mentioned some embodiments include institute in other embodiments
Including certain features rather than other feature, but the combination of the feature of different embodiment means in the scope of the present invention
Within and form different embodiments.For example, in claims above, embodiment claimed it is any it
One can in any combination mode come using.In addition, the information disclosed in the background technology section is intended only to deepen pair
The understanding of general background technology of the invention, and be not construed as recognizing or implying in any form that information composition has been this
The prior art well known to the technical staff of field.
Claims (10)
1. a kind of integrated portable nucleic acid-extracting apparatus, which is characterized in that including shell and be built in the shell controller,
Bar magnet, bar magnet set and test tube platform；
The bar magnet, bar magnet set and the test tube platform are set gradually from top to bottom, and the bar magnet passes vertically through the magnetic
Stick set can be placed in the test tube of the test tube platform；
The bar magnet, bar magnet set can oscilaltion, and translation can be controlled；The bar magnet set can shake；
Opening is arranged in the front side wall of the shell, and the test tube platform can be moved forward and backward, and passes in and out the shell from the opening
Body；
The controller is electrically connected with the bar magnet, bar magnet set respectively.
2. integrated portable nucleic acid-extracting apparatus according to claim 1, which is characterized in that the outer of the bar magnet set is set
It is equipped with multiple annular projections, the circumferentially extending that multiple annular projections are covered along the bar magnet, the outer profile of the annular projection
In round and smooth shape.
3. integrated portable nucleic acid-extracting apparatus according to claim 1, which is characterized in that the quantity of the bar magnet is more
It is a, the front and back distribution one by one of multiple bar magnets；
The quantity of the bar magnet set is multiple, multiple bar magnet set front and back distributions one by one；
Multiple rows of test tube is provided on the test tube platform, test tube described in each row is arranged in left-right direction, multiple rows of test tube edge
Front-rear direction is sequentially arranged.
4. integrated portable nucleic acid-extracting apparatus according to claim 1, which is characterized in that further include and the controller
The heating device of electrical connection；The heating device is set to the bottom on the test tube platform sinistral and/or right side, for test tube
Interior sample of nucleic acid is heated.
5. integrated portable nucleic acid-extracting apparatus according to claim 4, which is characterized in that the heating device with it is described
Test tube platform integrated setting, alternatively, the heating device, the test tube platform are detachably arranged with the test tube.
6. described in any item integrated portable nucleic acid-extracting apparatus according to claim 1~5, which is characterized in that further include seeing
Examine window；The observation window is arranged on the front side wall of the shell.
7. described in any item integrated portable nucleic acid-extracting apparatus according to claim 1~5, which is characterized in that further include control
Panel processed；The upper surface of the shell is arranged in the control panel, is electrically connected with the controller.
8. a kind of method for extracting nucleic acid based on integrated portable nucleic acid-extracting apparatus, which comprises the steps of:
Step 1: being in vitro put into sample of nucleic acid and magnetic bead to be extracted, starting heating device is heated；
Step 2: being stirred concussion to sample of nucleic acid using bar magnet set；
Step 3: to another test tube and eluting nucleic acid using bar magnet transfer magnetic bead.
9. method for extracting nucleic acid according to claim 8, which is characterized in that step 3 finish take out sample of nucleic acid it
Afterwards, sterilamp is opened to carry out disinfection sterilization to enclosure interior.
10. method for extracting nucleic acid according to claim 8, which is characterized in that in step 3, first exist before elution nucleic acid
Magnetic bead is cleaned at least one test tube.
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Inventor after: Wang Shengqi Inventor after: Xiao Rui Inventor after: Rong Zhen Inventor before: Wang Shengqi Inventor before: Xiao Rui Inventor before: Rong Zhen Inventor before: Zhang Lijie |
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Application publication date: 20190806 |