CN110093273B - Embryo time difference imaging culture module - Google Patents

Abstract

The invention discloses an embryo time difference imaging culture module, which comprises: a culture chamber body and a culture dish arranged in the culture chamber body; the culture dish comprises at least two culture ponds, wherein each culture pond comprises an upper pond body and a lower pond body, the inner wall of the upper pond body is connected with the inner wall of the lower pond body, and the inclination angle of the inner wall of the upper pond body is smaller than that of the inner wall of the lower pond body. According to the invention, by arranging more than two culture ponds, users can reasonably distribute the culture ponds according to the quantity of the cultured embryos so as to avoid unnecessary waste of culture solution; the inner wall of the upper part of the culture pond forms an obvious angle relative to the inner wall of the lower part, has obvious boundary characteristics, and is easier to identify and predict the liquid level position in the pipetting operation process of a user; the embryo micropore comprises an upper hole and a bottom hole, so that a user can conveniently and quickly position a micropore area and guide in embryos, meanwhile, the phenomenon of confusion caused by the fact that the embryos are accidentally separated from the outlet holes can be avoided, and the embryo micropore is safer and more reliable; the inner wall of the upper part of the culture pond is designed into an angle, so that the influence of concave moon on optical imaging is reduced.

Description

Embryo time difference imaging culture module

Technical Field

The invention relates to the technical field of biological sample cultivation, in particular to an embryo time difference imaging cultivation module.

Background

With the full release of national two-child policies, in vitro fertilization-embryo transfer (IVF-ET) has received more attention as a human assisted reproductive technology that can treat infertility. However, the lower pregnancy rate is one of the development bottlenecks of the auxiliary reproduction technology at present, how to improve the availability of embryos and obtain higher clinical pregnancy rate becomes a research hotspot in the auxiliary reproduction field.

The embryo time difference photographic monitoring and culturing system, which is a time difference culturing system for short, is an emerging embryo screening and evaluating method applied to clinic, has the characteristics of no artificial interference on a culturing mode and real-time monitoring, can dynamically observe embryos, evaluate the embryos by using morphological kinetic parameters, and enables screening results to be more objective. Therefore, the time difference culture system evaluates the development potential of the embryo through a time difference imaging technology, and screens out the embryo with the highest quality for transplantation, thereby effectively improving the clinical effect of the assisted reproduction technology, improving the implantation rate and the clinical pregnancy rate and shortening the successful pregnancy time of patients.

Embryo time difference imaging culture device is generally provided with an embryo culture module, wherein a culture chamber capable of being avoided is formed in the embryo culture module, and an embryo time difference imaging culture dish is arranged in the culture chamber and used for embryo culture.

The embryo time difference imaging culture dish is a special embryo carrier matched with a time difference culture system, is a transparent structure body formed by injection molding of plastic (such as polyester material), consists of a dish body and a dish cover, and is placed in a culture environment with strictly controlled temperature and CO2 concentration. The bottom of the culture dish is generally provided with a plurality of embryo hole sites, each hole site only accommodates a single embryo, and culture solution required by embryo development is stored in the dish. And a microscopic imaging system is arranged outside the culture environment to shoot and record each embryo image in the dish at intervals, so that a time-lapse album is manufactured for image algorithm identification and development evaluation.

The common embryo time difference culture has independent culture and shared culture modes, wherein the independent culture modes separate embryos in separate culture tanks, the common culture tanks with straight holes and taper holes are provided with micropores sinking in the middle of the bottom of the culture tank for storing single embryos, and all embryo culture tanks are distributed in a straight line or in an array. The shared culture mode is that all embryos are in the same culture pond, the bottom of the culture pond is sunk with a plurality of micropores and distributed in a certain geometrical characteristic, and the embryos are stored in the micropores. Both culture methods are to store culture solution in the lower part of the culture tank and cover the upper part with oil layer (such as mineral oil) to inhibit volatilization of the culture solution.

The shared culture mode is typified by a matched culture dish of an Embryoscope series time difference culture device of Unisense FertiliTech company, and because all embryos are in the same culture area, the use ratio of the culture holes of the embryos is the same, and the added culture solution is the same, so that the waste caused by redundancy of the use amount of the culture solution relative to the number of the embryos is easily caused. The culture dish for embryo sample development monitoring disclosed by the Australian Genea Limited company is also easy to have the defects, in addition, the embryo micropores are shallow and are Limited by the design of an optical system, the distance between adjacent micropores is particularly short, certain difficulty is brought to the operation of guiding the embryo into the micropores by a user, and special care is needed in the transfer process of the culture dish so as to avoid the situation that the embryo is separated from the micropores due to overlarge shaking amplitude and is confused with each other.

In addition, the culture hole of the time difference imaging culture dish has a common problem that the depth of the culture hole is shallow because the culture dish and the culture solution are transparent, so that a user can hardly accurately identify the liquid level position during pipetting, the repeatability of manual liquid feeding is difficult to control, and the culture solution of part of embryos is less or wastefully overflows.

In addition, the existing embryo culture module also has the problems of low cover closing precision of the cover opening and closing mechanism, reduced imaging quality and the like, and the use requirements of users are difficult to meet.

Disclosure of Invention

The technical problem to be solved by the invention is to provide an embryo time difference imaging culture module aiming at the defects in the prior art.

In order to solve the technical problems, the invention adopts the following technical scheme: an embryo jet lag imaging culture module, comprising: a culture chamber body and a culture dish arranged in the culture chamber body;

the culture dish comprises at least two culture ponds, wherein each culture pond comprises an upper pond body and a lower pond body, the inner wall of the upper pond body is connected with the inner wall of the lower pond body, the inclination angle of the inner wall of the upper pond body is smaller than that of the inner wall of the lower pond body, and the inclination angle refers to the included angle between the inner wall of the upper pond body or the inner wall of the lower pond body and the horizontal direction.

Preferably, the culture dish comprises a dish body and a dish cover, wherein the dish body comprises a round part and a rectangular part with the width smaller than the diameter of the round part, one side of the rectangular part is positioned in the round part, and the other side of the rectangular part extends out of the round part;

a culture area is arranged on the dish body, and the culture pond is arranged in the culture area;

the culture area is formed by surrounding a bottom plate of the dish body and a culture area coaming extending upwards from the bottom plate, and the bottom plate is concaved downwards to form a culture pond, a cleaning tank and a serial number mark.

The culture pond comprises an upper pond body and a lower pond body, wherein the inner wall of the upper pond body is connected with the inner wall of the lower pond body, and the inclination angle of the inner wall of the upper pond body is smaller than that of the inner wall of the lower pond body, wherein the inclination angle refers to the included angle between the inner wall of the upper pond body or the inner wall of the lower pond body and the horizontal direction.

Preferably, at least one sample micropore is arranged at the bottom of the lower cell body, the sample micropore comprises an upper hole and a bottom hole, the inner diameter of the upper hole is larger than that of the bottom hole, and the inner wall of the bottom hole has an included angle which is not 0 DEG with the vertical direction.

Preferably, the dish cover is used for being buckled on the dish body and comprises a cover plate and a dish cover coaming extending downwards from the cover plate, and when the dish cover is buckled on the dish body, the culture area coaming of the dish body is in the interior of the dish cover coaming.

Preferably, the dish cover further comprises a holding piece and a partition board, wherein the partition board extends out of the bottom surface of the dish cover and is parallel to the cover plate, and the holding piece is vertically arranged on the partition board.

Preferably, bosses are arranged at four top positions of the lower wall of the cover plate, and when the dish cover is buckled on the dish body, the cover plate is supported on the coaming of the culture area through the four bosses.

Preferably, the dish body is further provided with a PH measuring area, a trademark area, a label area and a handheld area.

Preferably, the culture chamber body comprises a culture chamber platform and a culture chamber upper cover, and the culture chamber platform and the culture chamber upper cover form a closed sample culture chamber; the culture room platform is provided with a dish groove for setting the culture dish, the shape of the dish groove is matched with the shape of the bottom surface of the culture dish, and a light-transmitting groove penetrating through the culture room platform is formed in the middle of the dish groove.

Preferably, the rear end of the upper cover of the culture chamber is connected with the culture chamber platform through a rotary hinge, an annular groove is formed in the bottom surface of the upper cover of the culture chamber, sealing strips are filled in the annular groove, and an opening and closing mechanism is further arranged at the front end of the culture chamber body;

the opening and closing mechanism comprises a baffle plate, an unlocking plate, a hinge shaft, a hinge seat, a second tension spring, a lock hook, a positioning block, a photoelectric switch, a reed and a reed pressing block which are arranged on the culture chamber platform;

the whole lock hook is L-shaped, a hinge hole, a tension spring hole and a hook head are sequentially formed in the lock hook from top to bottom along the length direction, a reverse edge is arranged at the bottom of the tail end of the hook head, and the lock hook is matched with the hinge shaft through the hinge hole and can be rotatably arranged on a hinge seat fixedly connected to the upper cover of the culture room;

one end of the second tension spring is connected with the tension spring hole, and the other end of the second tension spring is connected with the upright post arranged on the upper cover of the culture chamber;

the positioning block is fixed at the bottom of the upper cover of the culture chamber and is of a hollow structure, the lock hook penetrates through the positioning block from top to bottom, and the hook head at the bottom of the lock hook is exposed out of the positioning block.

Preferably, a rectangular hole is formed in the culture chamber platform below the latch hook, a bevel edge is arranged on a side wall of the rectangular hole corresponding to the reverse edge of the latch hook head, and the bottom of the bevel edge is hollowed out to form a latch hook groove;

the reed is arranged below the rectangular hole and is L-shaped, the short side of the reed is arranged below the hook head, one end of the long side of the reed is pressed by the reed pressing block, and the photoelectric switch is arranged below the short side of the reed;

the positioning block is further provided with a lug which is used for being inserted into the rectangular hole in a matched mode, two sides of the lug are provided with positioning straight edges which are used for being matched with two perpendicular inner walls of the rectangular hole, and after the rectangular hole is inserted into the lug, the positioning straight edges are located on two sides of the bevel edge of the rectangular hole.

Preferably, a spring pin is arranged on the bottom surface of the upper cover of the culture chamber, the spring pin comprises a pin sleeve, a pin and a pressure spring, the pin sleeve is cylindrical, a step round hole is formed in the pin sleeve, and the step round hole comprises a large round hole section and a small round hole section which are sequentially arranged from top to bottom; the lower end of the pin is a ball head and can be arranged in the small round hole section in a sliding way, the upper end of the pin is provided with a round step with the diameter larger than that of the small round hole section, and the round step can be arranged in the large round hole section in a sliding way; the pressure spring is arranged in the large round hole section, and the lower end of the pressure spring is propped against the round step;

the culture chamber platform is provided with a taper hole for the ball head of the pin to be inserted in a matched mode, and the taper hole and the pin are coaxial.

The beneficial effects of the invention are as follows: in the embryo time difference imaging culture module, by arranging more than two culture ponds, users can reasonably distribute according to the quantity of the cultured embryos so as to avoid unnecessary culture solution waste; in the invention, the upper inner wall of the culture pond forms an obvious angle relative to the lower inner wall, has obvious boundary characteristics, and is easier to identify and predict the liquid level position in the pipetting operation process of a user; in the invention, the embryo micropore comprises the upper hole and the bottom hole, so that a user can conveniently and quickly position the embryo to the micropore area and guide the embryo, and meanwhile, the confusion phenomenon caused by the fact that the embryo accidentally breaks away from the outlet hole can be avoided, and the method is safer and more reliable; in the invention, the inner wall of the upper part of the culture pond is designed into an angle, so that the influence of concave moon on optical imaging is reduced. According to the invention, the opening and closing mechanism is arranged on the culture chamber body, so that the accuracy of the closing cover position in the culture chamber body is high, and the reliable operation of the microscopic imaging system can be ensured.

Drawings

FIG. 1 is a schematic diagram of an embryo jet lag imaging culture module of the present invention;

FIG. 2 is a schematic diagram of the structure of the culture dish of the present invention;

FIG. 3 is a schematic diagram showing functional areas of the dish body according to the present invention;

FIG. 4 is a schematic view of the structure of the dish body of the present invention;

FIG. 5 is a schematic view of the structure of the capsule of the present invention;

FIG. 6 is a schematic view of the bottom structure of the capsule of the present invention;

FIG. 7 is a schematic cross-sectional view of a culture dish according to the invention;

FIG. 8 is a schematic view of the cooperation of the dish cover and the dish body according to the present invention;

FIG. 9 is a schematic view of the culture dish of the present invention in use;

FIG. 10 is a schematic diagram showing the construction of an embryo transit time imaging culture module according to the present invention applied to a specific embryo transit time culture device in one embodiment;

FIG. 11 is a schematic sectional view of the culture chamber body of the present invention;

FIG. 12 is a schematic view of a partial enlarged structure at A in FIG. 11 according to the present invention;

FIG. 13 is a schematic view of a partially enlarged structure of the present invention at B in FIG. 11;

FIG. 14 is a schematic view of the positioning block of the present invention;

fig. 15 is a schematic view of the rectangular hole structure of the present invention.

Reference numerals illustrate:

1-a culture chamber body; 2-a culture dish; 3-a dish body; 4-a dish cover; 5-a culture pond; 6, cleaning the tank; 7-marking the sequence number; 20-a culture zone; 21-a PH measurement zone; 22-trademark area; 23-tag region; 24-a hand-held region; 30—a circular portion; 31-rectangular section; 32-a bottom plate; 33-culture area enclosure; 40-cover plate; 41-dish cover coaming; 42-a hand piece; 43-separator; 44-a boss; 50-upper cell body; 51-lower cell body; 52-sample microwells; 53-upper hole; 54-bottom hole; 55-capping medium; 56-culture solution;

10-a culture chamber platform; 11-upper cover of culture room; 12-heating element, temperature sensor; 13, a heat preservation layer; 14-air inlet; 15-an air outlet; 16-a dish groove; 17-a hinge; 100-rectangular holes; 101-bevel edge; 102-a hook groove; 103-taper holes; 110-sealing strip; 111-stand columns; 112-spring pins; 113-pins; 114-pin sleeve; 115-a compression spring; 116-a step round hole;

8-an opening and closing mechanism; 80-a baffle; 800-flange; 81-unlocking plate; 82-hinge axis; 83-a hinge base; 84-a second tension spring; 85-latch hook; 86-positioning blocks; 87-photoelectric switch; 88-reed; 89-reed pressing; 850-hook heads; 851-chamfering; 860-bump; 861-positioning straight edges;

9-embryo time difference culture device; 90-light source; 91-a microscopic imaging system;

Detailed Description

The present invention is described in further detail below with reference to examples to enable those skilled in the art to practice the same by referring to the description.

It will be understood that terms, such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.

As shown in fig. 1-15, an embryo jet lag imaging culture module of the present embodiment includes: a culture chamber body 1 and a culture dish 2 arranged in the culture chamber body 1;

the culture dish 2 comprises a dish body 3 and a dish cover 4, wherein the dish body 3 comprises a circular portion 30 and a rectangular portion 31 with the width smaller than the diameter of the circular portion 30, one side of the rectangular portion 31 is positioned inside the circular portion 30, and the other side of the rectangular portion extends out of the circular portion 30. The overall appearance of the dish body 3 is formed by summing round and rectangular boolean, through the wide edges of the excircle and the rectangle of the dish body 3, the corresponding features of the dish groove 16 in the cooperation culture chamber body 1 can uniquely determine the placement position and angle, and the monitoring error of the embryo culture holes is reduced.

With reference to fig. 3, the dish body 3 is provided with a culture area 20, a PH measuring area 21, a trademark area 22, a tag area 23, and a hand-held area 24. Culture zone 20 is used for embryo manipulation and culture; the PH measuring area 21 stores embryo culture solution 56, and monitors the PH change condition of the culture solution 56 by measuring and monitoring the culture environment; trademark field 22 may customize product company logos, product names, etc.; the label area 23 attaches labels marked with patient information or bar codes, two-dimensional codes, etc. in machine-readable format according to the user's need; the holding area 24 of the dish body 3 allows the user to transfer and pick and place the culture dish 2 by pinching with the thumb and forefinger. In a preferred embodiment, the outer diameter of the outer circle of the dish body 3 is 55mm, the width of the holding area 24 of the dish body 3 is 32mm, the distance between the end face of the holding area 24 and the circle center is 36mm, and the height of the dish body 3 is 10mm.

Wherein, the culture area 20 is formed by a bottom plate 32 of the dish body 3 and a culture area coaming 33 extending upwards from the bottom plate 32, the bottom plate 32 is concaved downwards to form a culture pond 5, a cleaning tank 6 and a serial number mark 7; the culture pond 5 is used for storing embryos and culture solution 56, and the rinse tank 6 arranged beside the culture pond 5 is used for preparing the culture solution 56 or buffering or embryo rinsing during the liquid changing operation, and the serial number mark 7 is convenient for users to code and rapidly index the embryos.

Wherein, the culture area 20 of the dish body 3 comprises at least two culture ponds 5, in a preferred embodiment, 4 culture ponds 5 are included, the culture area 20 can hold 16 embryos at most, 16 embryos are distributed in 4 independent culture ponds 5 which are arranged in a straight line, each culture pond 5 holds 4 embryos, and the advantage is that a user can determine to utilize the first few culture ponds 5 according to the number of the embryos, and waste of the culture solution 56 in a shared culture mode of all the embryos can be reduced. For example, when the number of embryos is 9 to 12, only the first 3 culture ponds 5 are used, and when the number of embryos is 5 to 8, only the first 2 culture ponds 5 are used.

The culture pond 5 comprises an upper pond body 50 and a lower pond body 51, wherein the inner wall of the upper pond body 50 is connected with the inner wall of the lower pond body 51, and the inclination angle of the inner wall of the upper pond body 50 is smaller than that of the inner wall of the lower pond body 51, wherein the inclination angle refers to the included angle between the inner wall of the upper pond body 50 or the inner wall of the lower pond body 51 and the horizontal direction. The lower cell body 51 is used for storing embryo culture fluid 56, and the upper cell body 50 is used for covering a volatilization-preventing medium, wherein the upper inner wall is connected with the lower inner wall and forms a obvious angle relative to the lower inner wall. In a preferred embodiment, the total depth of the culture tanks 5 is about 4mm, the lower depth is 3mm, the upper depth is 1mm, the upper and lower inner walls are angled at about 50 degrees, each culture tank 5 comprises 4 embryos and can hold a volume of culture solution 56 of about 150 to 160 microliters.

Because the upper part and the lower part of the culture pond 5 are in obvious angles, the boundaries of the upper part and the lower part are clearer, and the distance between the liquid level and the boundary can be easily identified and predicted in the pipetting operation process of a user and can react in advance.

In general, due to the influence of intermolecular forces of contact surfaces and surface tension of liquid, obvious concave moon surfaces are formed at the contact positions of the liquid and the pore walls, and potential influence is caused on optical imaging, such as in a straight pore culture mode. Avoiding the influence of the concave surface, the embryo can be kept away from the inner wall by increasing the space of the culture pond 5, but the overall size of the dish body 3 can be increased, and the space utilization rate is low. In this embodiment, the upper inner wall of the culture pond 5 is connected with the lower inner wall, and forms an obvious angle relative to the lower inner wall, and another beneficial effect is that the concave moon surface of the upper inner wall of the culture pond 5 and the covering medium 55 have larger radius and are offset outwards due to the existence of the inclination angle, so that the irradiation area of incident light is better avoided, the imaging effect is improved, and the dish body 3 has more compact structure.

The bottom of the lower cell body 51 is provided with at least one sample microwell 52, the sample microwell 52 comprises an upper hole 53 and a bottom hole 54, the inner diameter of the upper hole 53 is larger than the inner diameter of the bottom hole 54, and the inner wall of the bottom hole 54 has an included angle of not 0 DEG with the vertical direction. The bottom hole 54 has an inner wall that is at a significant angle to vertical. In a preferred embodiment, the sample wells 52 are equally spaced at 2mm intervals, wherein the upper well 53 has an inner diameter of 1.6mm and a depth of 0.6mm, the bottom well 54 has a bottom diameter of 0.4mm and a depth of 0.6mm, the inner wall is angled at about 15 degrees from vertical, and the junctions are rounded. Because the inner diameter of the upper hole 53 is larger, a user can quickly position the upper hole 53 first when guiding an embryo into or out of the bottom hole 54, and the angle design between the inner wall of the bottom hole 54 and the vertical direction also plays a good guiding role. In addition, if the operation is careless, the embryo will generally only separate from the bottom hole 54 and enter the upper hole 53 area, but will not enter the public area, so that the phenomenon of confusion is more safe and reliable.

The dish cover 4 is used for being buckled on the dish body 3, and it includes apron 40 and dish cover bounding wall 41 that extends downwards from apron 40, and when dish cover 4 lock was on the dish body 3, the culture section bounding wall 33 of the dish body 3 was in the inside of dish cover bounding wall 41.

The dish cover 4 further comprises a holding piece 42 and a partition plate 43, wherein the partition plate 43 extends from the bottom surface of the dish cover 4 and is parallel to the cover plate 40, and the holding piece 42 is vertically arranged on the partition plate 43. The function of the baffle 43 is to isolate the potential contamination of the hands to the inside of the coaming when the user holds the dish cover 4, thereby improving the safety.

Bosses 44 are arranged at four top positions of the lower wall of the cover plate 40, and when the dish cover 4 is buckled on the dish body 3, the cover plate 40 is supported on the culture area coaming 33 through the four bosses 44. The dish cover 4 is lifted by the bosses 44 everywhere, so that a gap is reserved between the lower wall of the cover plate 40 and the top wall of the culture area coaming 33, and culture gas is allowed to enter the culture area 20 for exchange.

The culture dish 2 can adopt a transparent structure body formed by injection molding of plastics (such as polystyrene material and polyester material), the principle of injection molding process is satisfied, the thickness of the cross section of the structure is about 1mm, and the structure can be slightly thicker or slightly thinner at different positions so as to realize local characteristic requirements.

In one embodiment, referring to fig. 1, the culture chamber platform 10 and the culture chamber upper cover 11 are both provided with heating elements and temperature sensors, and are independently temperature-controlled, the culture chamber upper cover 11 and the platform are made of aluminum alloy materials with high thermal conductivity, and the back surfaces of the culture chamber upper cover 11 and the platform are respectively covered with a heat preservation layer 13 to reduce heat loss of the structure. Through redundant control by temperature change design, guarantee even one side under the condition that breaks down, the opposite side can normally work, is unlikely to the environment in the culture room to appear deteriorating in the short time, reserves sufficient time and shifts culture dish 2. The culture room platform 10 and the upper cover are also respectively provided with an air inlet 14 and an air outlet 15. Is used for the inlet and outlet of CO2, O2 and N2 mixed gas.

The culture module comprises a culture chamber platform and a culture chamber upper cover, and the culture chamber platform and the culture chamber upper cover form a closed sample culture chamber; the culture room platform is provided with a dish groove 16 for arranging the culture dish 2, the shape of the dish groove 16 is matched with the shape of the bottom surface of the culture dish 2, and the middle of the dish groove 16 is provided with a light-transmitting groove penetrating through the culture room platform.

In one embodiment, referring to 11-15, the rear end of the upper cover 11 of the culture chamber is connected with the platform 10 of the culture chamber through a rotary hinge 17, the bottom surface of the upper cover 11 of the culture chamber is provided with an annular groove, the annular groove is filled with a sealing strip 110, and the front end of the culture module 1 is also provided with an opening and closing mechanism 8. The rotary hinge 17 is selected as a constant torque hinge 17, and the upper cover can stay at any position after being lifted.

The opening and closing mechanism 8 adopts a lever principle and comprises a baffle plate 80, an unlocking plate 81, a hinge shaft 82, a hinge seat 83, a tension spring, a latch hook 85, a positioning block 86, a photoelectric switch 87, a reed 88 and a reed pressing block 89 which are arranged on the culture chamber platform 10;

the whole latch hook 85 is L-shaped, a hinge 17 hole, a tension spring hole and a hook head 850 are sequentially arranged on the latch hook 85 from top to bottom along the length direction, a reverse edge 851 is arranged at the bottom of the tail end of the hook head 850, the latch hook 85 is matched with the hinge shaft 82 through the hinge 17 hole, and the latch hook 85 is rotatably arranged on a hinge seat 83 fixedly connected to the upper cover 11 of the culture room;

one end of the second tension spring 84 is connected with the tension spring hole, and the other end is connected with a stand column 111 arranged on the upper cover 11 of the culture chamber;

the positioning block 86 is fixed at the bottom of the upper cover 11 of the culture chamber, and is of a hollow structure, the latch hook 85 passes through the positioning block 86 from top to bottom, and the hook head 850 at the bottom of the latch hook 85 exposes the positioning block 86. The locating block 86 is fixed at the bottom of the upper cover 11 of the culture chamber, and due to the action of the tension spring, the bottom of the lock hook 85 is attached to the inner wall of the locating block 86 by default. The unlocking plate 81 is fixedly connected with the hinge shaft 82, and when the unlocking plate 81 is shifted along the direction of the arrow shown in the drawing, the tension of the tension spring is overcome, so that the locking hook 85 rotates around the hinge shaft 82. Baffle 80 is fixed in culture chamber upper cover 11 upper portion, and baffle 80 is close to the one side of unlocking plate 81 and is flange 800 (for example, the accessible sets up fluting 801 on baffle 80, and the trailing edge of fluting 801 forms the baffle), through the size design of unlocking plate 81, realizes easily that stirring under the circumstances that unlocking plate 81 withstood flange 800, latch hook 85 bottom can not bump the inner wall of locating piece 86 to protection latch hook 85, simultaneously through unlocking plate 81 and flange 800's effect of force, very easily lifts culture chamber upper cover 11, plays the purpose of uncapping.

In the above embodiment, the rectangular hole 100 is formed below the latch hook 85 on the culture chamber platform 10, the side wall of the rectangular hole 100 corresponding to the inverted edge 851 of the hook head 850 is provided with the inclined edge 101, and the bottom of the inclined edge 101 is hollowed to form the hook slot 102; when the cover is closed, under the action of the downward guide of the chamfer 851 of the hook head 850 and the inclined side 101 of the square hole 472 and the tension spring, the inner side of the hook head 850 of the lock hook 85 can be smoothly embedded into the hook groove 102, and the hook head 850 can reliably contact with the groove surface under the pretightening force of the sealing strip 110.

The reed 88 is disposed below the rectangular hole 100, and has an L-shape, the short side of the reed 88 is disposed below the hook head 850, one end of the long side of the reed 88 is pressed by the reed pressing block 89, and the photoelectric switch 87 is disposed below the short side of the reed 88. One end of the spring 88 is pressed by the spring pressing block 89, and the other end is kept in a horizontal state when not being stressed and is bent downwards when being stressed. When the cover is closed, the hook head 850 of the lock hook 85 is embedded into the hook groove 102, the back of the hook head 850 can prop against the tail end of the reed 88, so that the reed 88 is pressed down and deformed, and the short side of the reed 88 can trigger the photoelectric switch 87, thereby the system can identify the opening and closing state of the culture chamber.

The positioning block 86 is further provided with a protruding block 860 for being inserted into the rectangular hole 100 in a matching manner, four corners of the protruding block 860 are chamfered 851, positioning straight edges 861 for being matched with two vertical inner walls of the rectangular hole 100 are arranged on two sides of the protruding block 860, and after the rectangular hole 100 is inserted into the protruding block 860, the positioning straight edges 861 are positioned on two sides of the inclined edge 101 of the rectangular hole 100. The positioning straight edge 861 can be matched with the inner wall of the square hole 472 when the cover is closed, so that the positioning function of the upper cover 11 of the culture chamber relative to the platform 10 of the culture chamber in the transverse direction is realized.

Because the open-close hinge 17 of the upper cover 11 of the culture chamber works for a long time and wears, the open-close hinge 17 wears to generate gaps, and the position of the upper cover 11 of the culture chamber relative to the platform 10 of the culture chamber is uncertain after the cover is closed, so that the light source 90 and the imaging device 21 deviate from the original design positions, and in severe cases, the coaxial relationship is destroyed, the imaging quality is reduced, and the images cannot be identified. This problem can be effectively structured by the scheme in the following embodiment.

In a further embodiment, referring to fig. 13, the bottom surface of the upper cover 11 of the culture chamber is provided with a spring pin 112, the spring pin 112 comprises a pin sleeve 114, a pin 113 and a pressure spring 115, the pin sleeve 114 is cylindrical, a step round hole 116 is formed in the pin sleeve, and the step round hole 116 comprises a large round hole section and a small round hole section which are sequentially arranged from top to bottom; the lower end of the pin 113 is a ball head and is slidably arranged in the small round hole section, the upper end of the pin 113 is provided with a round step with the diameter larger than that of the small round hole section, and the round step is slidably arranged in the large round hole section; the pressure spring 115 is arranged in the large round hole section, and the lower end is propped against the round bench; the pin 113 is provided with a certain initial preload.

The culture chamber platform 10 is provided with a taper hole 103 for the ball head of the pin 113 to be inserted in a matching way, and the taper hole 103 is coaxial with the pin 113. Spring pin 112 and tapered bore 103 to eliminate the effects of play in the wear of hinge 17.

The spring pin 112 and the taper hole 103 are respectively embedded in the upper cover 11 of the culture chamber and the platform 10 of the culture chamber, and are coaxial, the ball head of the pin 113 and the taper hole 103 play a role in guiding and positioning when the cover is closed, so that the position deviation of the upper cover 11 of the culture chamber and the platform in the horizontal plane at the point can be limited, and the influence of a gap is eliminated. The compression spring 115 ensures that the pin 113 is always in a contact engagement with the tapered bore 103 even if the gap between the hinges 17 increases.

The positioning of the spring pin 112 and the tapered hole 103 post cannot limit the rotation offset of the upper cover 11 of the culture chamber relative to the platform, but the position relationship of the upper cover 11 of the culture chamber and the platform can be uniquely determined by combining the transverse positioning function of the positioning block 86 and the square hole 472, so that the gap influence of the abrasion of the hinge 17 is thoroughly eliminated, and the reliability of the imaging quality is ensured.

The embryo time difference imaging culture module of the invention can be mainly used in embryo time difference culture devices for embryo incubation, and the embryo time difference culture devices are also generally provided with a light source and a microscopic imaging system for shooting and recording time difference images in the embryo culture process. Referring to fig. 10, the embryo transit time imaging culture module of the present invention is applied to one illustration in a specific embryo transit time culture device 9. Embryos of the culture dish 2 are distributed in at least two independent culture ponds 5, the culture ponds 5 are arranged in a straight line, the system structure is compact, the degree of freedom of movement is small, a microscopic imaging system 91 is responsible for shooting and recording time difference images in the embryo culture process, a light source 90 is located on the upper side of the culture dish 2 and can independently move along an X axis, a microscopic imaging device is located on the lower side of the culture dish 2 and can independently move along the X axis and independently move along a Z axis, the culture ponds 5 are respectively moved along the X axis and positioned to a sample area to be detected, imaging focal planes are switched through Z-axis movement of the imaging device, and then sequential imaging of all samples is achieved.

In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present invention.

Although embodiments of the present invention have been disclosed above, it is not limited to the use of the description and embodiments, it is well suited to various fields of use for the invention, and further modifications may be readily apparent to those skilled in the art, and accordingly, the invention is not limited to the particular details without departing from the general concepts defined in the claims and the equivalents thereof.

Claims (7)

1. An embryo jet lag imaging culture module, comprising: a culture chamber body and a culture dish arranged in the culture chamber body;

the culture dish comprises at least two culture ponds, wherein each culture pond comprises an upper pond body and a lower pond body, the inner wall of the upper pond body is connected with the inner wall of the lower pond body, and the inclination angle of the inner wall of the upper pond body is smaller than that of the inner wall of the lower pond body, wherein the inclination angle refers to the included angle between the inner wall of the upper pond body or the lower pond body and the horizontal direction;

the bottom of the lower tank body is provided with at least one sample micropore, the sample micropore comprises an upper hole and a bottom hole, the inner diameter of the upper hole is larger than that of the bottom hole, and the inner wall of the bottom hole has an included angle which is not 0 DEG with the vertical direction;

the culture chamber body comprises a culture chamber platform and a culture chamber upper cover, and the culture chamber platform and the culture chamber upper cover form a closed sample culture chamber; the culture room platform is provided with a dish groove for arranging the culture dish, the shape of the dish groove is matched with the shape of the bottom surface of the culture dish, and a light-transmitting groove penetrating through the culture room platform is arranged in the middle of the dish groove;

the rear end of the upper cover of the culture chamber is connected with the culture chamber platform through a rotary hinge, an annular groove is formed in the bottom surface of the upper cover of the culture chamber, sealing strips are filled in the annular groove, and an opening and closing mechanism is further arranged at the front end of the culture chamber body;

the opening and closing mechanism comprises a baffle plate, an unlocking plate, a hinge shaft, a hinge seat, a second tension spring, a lock hook, a positioning block, a photoelectric switch, a reed and a reed pressing block which are arranged on the culture chamber platform;

the whole lock hook is L-shaped, a hinge hole, a tension spring hole and a hook head are sequentially formed in the lock hook from top to bottom along the length direction, a reverse edge is arranged at the bottom of the tail end of the hook head, and the lock hook is matched with the hinge shaft through the hinge hole and can be rotatably arranged on a hinge seat fixedly connected to the upper cover of the culture room;

one end of the second tension spring is connected with the tension spring hole, and the other end of the second tension spring is connected with the upright post arranged on the upper cover of the culture chamber;

the positioning block is fixed at the bottom of the upper cover of the culture chamber and is of a hollow structure, the lock hook penetrates through the positioning block from top to bottom, and the hook head at the bottom of the lock hook is exposed out of the positioning block.

2. The embryo time lapse imaging culture module of claim 1, wherein the culture dish comprises a dish body and a dish cover, the dish body comprises a circular portion and a rectangular portion with a width smaller than the diameter of the circular portion, one side of the rectangular portion is positioned inside the circular portion, and the other side extends out of the circular portion;

a culture area is arranged on the dish body, and the culture pond is arranged in the culture area;

the culture area is formed by surrounding a bottom plate of the dish body and a culture area coaming extending upwards from the bottom plate, and the bottom plate is concaved downwards to form a culture pond, a cleaning tank and a serial number mark.

3. The embryo time lapse imaging culture module of claim 2, wherein the dish cover is adapted to snap-fit onto the dish body and comprises a cover plate and a dish cover enclosure plate extending downwardly from the cover plate, wherein the culture zone enclosure plate of the dish body is inside the dish cover enclosure plate when the dish cover is snapped onto the dish body.

4. The embryo time lapse imaging culture module, as set forth in claim 3, wherein the capsule further comprises a hand piece and a spacer plate, the spacer plate extending from the bottom surface of the capsule parallel to the cover plate, the hand piece being disposed vertically on the spacer plate;

the four top positions of the lower wall of the cover plate are respectively provided with a boss, and when the dish cover is buckled on the dish body, the cover plate is supported on the coaming of the culture area through the four bosses.

5. The embryo time lapse imaging culture module of claim 4, wherein the dish further comprises a PH measurement zone, a trademark zone, a label zone, and a hand-held zone.

6. The embryo time difference imaging culture module according to claim 1, wherein a rectangular hole is formed below the latch hook on the culture chamber platform, a bevel edge is arranged on a side wall of the rectangular hole corresponding to the reverse edge of the latch head, and the bottom of the bevel edge is hollowed out to form a latch slot;

the reed is arranged below the rectangular hole and is L-shaped, the short side of the reed is arranged below the hook head, one end of the long side of the reed is pressed by the reed pressing block, and the photoelectric switch is arranged below the short side of the reed;

the positioning block is further provided with a lug which is used for being inserted into the rectangular hole in a matched mode, two sides of the lug are provided with positioning straight edges which are used for being matched with two perpendicular inner walls of the rectangular hole, and after the rectangular hole is inserted into the lug, the positioning straight edges are located on two sides of the bevel edge of the rectangular hole.

7. The embryo time difference imaging culture module according to claim 6, wherein a spring pin is arranged on the bottom surface of the culture chamber upper cover, the spring pin comprises a pin sleeve, a pin and a pressure spring, the pin sleeve is cylindrical, a step round hole is formed in the pin sleeve, and the step round hole comprises a large round hole section and a small round hole section which are sequentially arranged from top to bottom; the lower end of the pin is a ball head and can be arranged in the small round hole section in a sliding way, the upper end of the pin is provided with a round step with the diameter larger than that of the small round hole section, and the round step can be arranged in the large round hole section in a sliding way; the pressure spring is arranged in the large round hole section, and the lower end of the pressure spring is propped against the round step;

the culture chamber platform is provided with a taper hole for the ball head of the pin to be inserted in a matched mode, and the taper hole and the pin are coaxial.