CN110082334B - Multichannel optical fiber fluorescence sensor - Google Patents
Multichannel optical fiber fluorescence sensor Download PDFInfo
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N21/643—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" non-biological material
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
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Abstract
The invention provides a multichannel optical fiber fluorescence sensor which comprises a light source, an excitation light splitting device, a signal light splitting device, a probe array, a filtering device, an imaging system and a control and data display terminal, wherein the signal light splitting device is connected with the excitation light splitting device, the probe array and the filtering device are respectively connected with the signal light splitting device, the imaging system is connected with the filtering device, the control and data display terminal is respectively connected with the light source and the imaging system in a communication mode, and the probe array comprises an optical fiber probe which is provided with a bare core area and quantum dots. Excitation light emitted by a light source respectively enters different optical fiber probes through coupling of a light splitting device, quantum dots on the evanescent wave excitation probes generated on the surfaces of the probes generate fluorescent signals, signal light of the fluorescent signals is collected by the optical fiber probes and is coupled into a filtering module through the light splitting device, multichannel fluorescent signal collection is realized, the detection structure can be visually displayed in an imaging system, the cost is relatively low, the volume is relatively small, the carrying is convenient, and the result can be obtained rapidly.
Description
Technical Field
The invention relates to biochemical sensing equipment, in particular to a multichannel optical fiber fluorescence sensor.
Background
The optical fiber fluorescence sensor is a novel sensor which organically combines a fluorescence detection technology and an optical fiber sensing technology, the sensor uses optical fibers as a light transmission carrier, and a far-end probe is used for detecting a sample and collecting fluorescence signals.
However, most of the existing optical fiber fluorescence sensors are single detection channels, and have the defects of single detection amount, limited detection substance types, low detection efficiency and the like. Along with the improvement of the requirements of the biochemical detection field on the sample detection speed, the development of multichannel optical fiber fluorescence detection technology and instrument is urgent.
The existing multichannel optical fiber fluorescence sensor implementation schemes mainly have two types: one is based on multi-detector implementation, and the other is based on optical fiber spectrometer implementation sensing; the former has complex system, relatively high cost, large volume, inconvenient carrying and difficult application to field detection; the latter intensity information is not intuitive and it is difficult to obtain the result quickly.
In view of this, the applicant has conducted intensive studies on a multichannel optical fiber fluorescence sensor, and has produced the present invention.
Disclosure of Invention
The invention aims to provide a multichannel optical fiber sensor which has relatively low cost, relatively small volume, convenient carrying and rapid acquisition of results.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
the utility model provides a multichannel optical fiber fluorescence sensor, includes light source, with the excitation light beam splitter that the light source is connected, with the signal light beam splitter that the excitation light beam splitter is connected, respectively with probe array and the filter equipment that the signal light beam splitter is connected, with the imaging system that the filter equipment is connected and respectively with the light source with imaging system communication is connected control and data display terminal, the probe array includes more than two optical fiber probe, optical fiber probe has the naked core district of biological activation treatment, with the quantum dot that the naked core district is connected and be used for discernment thing to be examined.
As an improvement of the invention, the filtering device comprises more than two bandpass filters which are arranged in combination with each other.
As an improvement of the invention, the imaging system comprises a lens arranged at the outlet end of the light filtering device and a CMOS image sensor or a CCD image sensor matched with the lens, wherein the CMOS image sensor or the CCD image sensor is in wireless communication connection with the control and data display terminal.
As an improvement of the present invention, the light source, the excitation light splitting device, and the signal light splitting device are detachably connected to the respective optical fiber probes through optical fiber connectors.
As an improvement of the invention, the light source is an LED light source, a solid state laser or a semiconductor laser with an output pigtail, which is a single-mode or multimode optical fiber.
As an improvement of the present invention, the excitation light splitting device includes a 1×n optical fiber coupler, a 1×n optical fiber beam splitter, or a 1×n optical fiber wavelength division multiplexer, the signal light splitting device includes N2×1 optical fiber couplers, 2×1 optical fiber loop devices, or 2×1 optical fiber wavelength division multiplexers, where N is a natural number, and N is greater than or equal to the number of the optical fiber probes, an input end of the 1×n optical fiber coupler, the 1×n optical fiber beam splitter, or the 1×n optical fiber wavelength division multiplexer is connected to the light source, N output ends are respectively connected to one first port of each 2×1 optical fiber coupler, each 2×1 optical fiber loop device, or each 2×1 optical fiber wavelength division multiplexer, another first port of each 2×1 optical fiber coupler, each 2×1 optical fiber loop device, or each 2×1 optical fiber wavelength division multiplexer is respectively connected to the optical fiber probe one-to-one second port.
As an improvement of the present invention, the device further comprises a housing, wherein the light source, the excitation light beam splitting device, the signal light beam splitting device, the filtering module and the imaging system are packaged in the housing, and the control and data display terminal is packaged in the housing or is located outside the housing.
As an improvement of the present invention, the excitation light beam splitter and the signal light beam splitter are connected to each other to form an optical fiber beam splitter.
As an improvement of the invention, the control and data display terminal is a mobile phone, a tablet computer or a microcomputer.
By adopting the technical scheme, the invention has the following beneficial effects:
1. through setting up beam split device and probe array, the excitation light that is sent by the light source gets into different fiber probes respectively through beam split device coupling, the quantum dot on the evanescent wave excitation probe that produces on the probe surface produces fluorescent signal, the signal light of this fluorescent signal is collected by fiber probes and is coupled into the filter module through beam split device, realize multichannel fluorescent signal acquisition, but its detection structure visual display is in imaging system, compare with current multichannel fiber fluorescence sensor implementation scheme, the cost is lower relatively, the volume is less relatively, convenient to carry and can acquire the result rapidly.
2. The multichannel optical fiber fluorescence sensor provided by the invention can realize the convenient, quick and accurate measurement of various substances (heavy metal ions, bacteria, toxins and the like), and is a small, efficient and convenient sensor applicable to various biochemical detection fields.
The multichannel optical fiber fluorescence sensor provided by the invention has the advantages of relatively simple optical path, relatively good stability and relatively high detection precision.
4. Through wireless communication connection, real-time remote detection can be realized.
Drawings
FIG. 1 is a schematic diagram of a multichannel fiber fluorescence sensor according to the present invention.
The labels correspond to the following:
10-a light source; 20-an excitation light beam splitter;
30-a signal light beam splitting device; 40-probe array;
41-optical fiber probe; 50-a light filtering device;
a 60-imaging system; 70-control and data display terminals.
Detailed Description
The invention will be further described with reference to the accompanying drawings and specific examples.
As shown in fig. 1, the multichannel optical fiber fluorescence sensor provided in this embodiment includes a light source 10 having a blue-violet wavelength, an excitation light splitting device 20 connected to the light source 10, a signal light splitting device 30 connected to the excitation light splitting device 20, a probe array 40 and a filter device 50 respectively connected to the signal light splitting device 30, an imaging system 60 connected to the filter device 50, and a control and data display terminal 70 communicatively connected to the light source 10 and the imaging system 60 respectively, wherein the excitation light splitting device 20 and the signal light splitting device 30 are connected to each other to form an optical fiber splitting device.
The light source 10 is mainly used for exciting the quantum dots to generate fluorescence, that is, the light source 10 is mainly used for providing excitation light required for fluorescence excitation of the quantum dots, and may be any one of an LED light source with an output pigtail, a semiconductor laser and a solid state laser, preferably, in this embodiment, the light source 10 is an LED light source with an output pigtail, and the output pigtail is a single-mode or multimode optical fiber, and may be coupled with various optical fiber devices through an optical fiber connector.
The probe array 40 includes two or more optical fiber probes 41 (four are shown in fig. 1 as an example) which are independently arranged, the specific number of the optical fiber probes 41 can be set according to actual needs, each optical fiber probe 41 has a bare core area which is subjected to biological activation treatment, quantum dots connected with the bare core area and biomolecules for identifying the to-be-detected object, wherein the quantum dots are mainly used for modifying the to-be-detected biochemical sample, and specifically, each optical fiber probe 41 is modified by a conventional method respectively with biomolecules capable of specifically identifying the specific to-be-detected object and quantum dots for fluorescence indication, so that the content of the to-be-detected object can be determined by the intensity of fluorescence signals of the quantum dots, of course, the specificity of the to-be-detected object which can be identified by the biomolecules on each optical fiber probe 41 is different from each other, the bare core area is a section of single-mode or multi-mode optical fiber section exposed outside the optical fiber probe 41, the optical fiber section is obtained by adopting a chemical etching method, a melt tapering method or a grinding method, and is used for connecting the to-be-detected object and the quantum dots by biological activation treatment, and the conventional method is described in a method, and the detailed method is not implemented here.
The optical fiber spectroscopic device is mainly used for coupling excitation light provided by the light source 10 into the optical fiber probe 41 and reversely coupling signal light collected by the optical fiber probe 41 to the optical filter device 50. As described above, the optical fiber spectroscopic apparatus includes the excitation light spectroscopic apparatus 20 and the signal light spectroscopic apparatus 30, the excitation light spectroscopic apparatus 20 includes a 1×n optical fiber coupler, a 1×n optical fiber beam splitter, or a 1×n optical fiber wavelength division multiplexer, which are commercially available, for equally dividing the excitation light, and in this embodiment, the excitation light spectroscopic apparatus 20 is described as including a 1×n optical fiber coupler, in which 1×n means that the optical fiber coupler has one input end and N output ends; the signal light splitting device includes N2×1 optical fiber couplers, 2×1 optical fiber loop devices or 2×1 optical fiber wavelength division multiplexers which are arranged independently of each other and are available directly from the market, and in this embodiment, the signal light splitting device is described by taking the signal light splitting device including N2×1 optical fiber couplers as an example, N2×1 optical fiber coupler arrays are arranged, which can couple excitation light into the optical fiber probe 41 and transmit the signal light collected by the optical fiber probe 41 to the filtering device 50 in a reverse coupling manner, and 2×1 in the 2×1 optical fiber couplers means that the optical fiber coupler has two first ports and one second port, and it is to be noted that N is a natural number, and N is greater than or equal to the number of the optical fiber probes 41. The input end of the 1×n fiber coupler, the 1×n fiber splitter, or the 1×n fiber wavelength division multiplexer is connected to the light source 10, the N output ends are connected to one of the first ports of the 2×1 fiber couplers, the 2×1 fiber loops, or the 2×1 fiber wavelength division multiplexers, the other first port of the 2×1 fiber coupler, the 2×1 fiber loops, or the 2×1 fiber wavelength division multiplexer (the first port is used as the output end of the signal light splitting device 30) is connected to the input end of the filter device 50, the second port is connected to the optical fiber probes 41, and of course, when N is greater than the number of the optical fiber probes 41, the second port of the 2×1 fiber coupler, the 2×1 fiber loop, or the 2×1 fiber wavelength division multiplexer is not connected to the optical fiber probes 41.
The filtering device 50 includes more than two bandpass filters, which are arranged in combination, and are used for filtering excitation light from the multipath fluorescent signals to allow the signal light to pass through, and filtering stray light existing in the signal light, wherein the passband wavelength of the bandpass filter is a filter corresponding to the fluorescence wavelength of the quantum dots. The number of specific filters and the specific arrangement structure thereof are conventional structures, so long as the function of filtering excitation light in the multipath fluorescent signals and allowing the signal light to pass through can be realized, and detailed description thereof is omitted herein. Of course, instead of the above-mentioned optical filter, a microfilter, a fiber bragg grating, or a fiber filter that can achieve the same function may be used. In addition, in the present embodiment, the outgoing end of the signal light from the optical fiber spectroscopic device is taken as the input end of the optical filter device 50, and the corresponding other end is taken as the output end.
The imaging system 60 includes a lens disposed at an outlet end of the filtering device 50 and a CMOS image sensor or a CCD image sensor cooperating with the lens, which is mainly used for collecting light spot images of multiple fluorescent signals, wherein the CMOS image sensor or the CCD image sensor is connected with the control and data display terminal 70 in a wireless communication manner, so that collected images can be transmitted to the control and data display terminal 70 in real time through wireless transmission and image signal processing is performed to obtain concentration information of the object to be detected. It should be noted that, corresponding components on mobile intelligent devices such as a mobile phone, a tablet or a digital camera may be directly adopted as the imaging system 60.
The control and data display terminal 70 is used for controlling the whole sensor, reading, processing and displaying the image transmitted by the imaging system 60, and analyzing the light intensity information of each light spot in the image to obtain the concentration information of the corresponding detection object, so as to finally generate a detection report. The control and data display terminal 70 may employ a conventional terminal, and may even directly use a mobile phone, a tablet computer, or a microcomputer (including raspberry group) as the control and data display terminal 70.
Preferably, in the present embodiment, the light source 10, the excitation light beam splitter 20, the signal light beam splitter 30 and the optical fiber probes 41 are detachably connected through optical fiber connectors, so as to ensure that the whole optical path is of an all-fiber structure, and the signal transmission is stable and reliable; the specific fiber optic connector may be selected according to the actual needs, such as an FC-type fiber optic connector, an SMA 905-type fiber optic connector, an ST-type fiber optic connector, or an SC-type fiber optic connector.
In addition, the multichannel fiber fluorescence sensor provided in this embodiment further includes a housing (not shown in the drawings), in which the light source 10, the excitation light splitting device 20, the signal light splitting device 30, the filter module 50, the imaging system 60 and the control and data display terminal 70 are packaged, so that the multichannel fiber fluorescence sensor is convenient to carry, and of course, the probe array 40 needs to be located outside the housing and connected to the fiber connector on the housing through the optical fibers. If necessary, the control and data display terminal 70 may not be enclosed in a housing, i.e., the light source 10, the excitation light splitting device 20, the signal light splitting device 30, the filter module 50 and the imaging system 60 are enclosed in a housing, and the control and data display terminal 70 is enclosed in a housing or outside a housing.
The multichannel optical fiber fluorescence sensor of the embodiment realizes multichannel sensing through the optical fiber probe array 40, has portability, is a multichannel optical fiber fluorescence sensing system which can be applied to the fields of environment detection, biochemical detection, food safety and the like, has important research value and good commercial popularization, and can realize excitation and signal collection of a plurality of optical fiber probes 41 by utilizing an optical fiber spectroscopic device when in use, acquire fluorescent signal images by utilizing an image sensing mode, control the system and acquire and process signals by utilizing the control and data display terminal 70, realize simultaneous detection of a plurality of objects to be detected, and finally realize real-time stable monitoring and instant generation of detection reports of a plurality of harmful substances.
For detecting Hg in aqueous solutions 2+ The multichannel optical fiber fluorescence sensor of omethoate, microcystin-LR and escherichia coli is taken as an example, a light source 10 adopts a small semiconductor laser with tail fibers, the center wavelength of the semiconductor laser is 405nm, the fiber output power of the semiconductor laser is 10mW, and the output tail fibers are multimode optical fibers with fiber cores and cladding diameters of 105 mu m and 125 mu m respectively; in the optical fiber spectroscopic device, the excitation light spectroscopic device 20 is implemented by adopting a 1×4 single-mode fiber coupler, and is used for splitting the excitation light in equal ratio, the signal light spectroscopic device 30 is implemented by adopting four 2×1 single-mode fiber coupler arrays, so that excitation light can be coupled into the optical fiber probe 41, and signal light collected by the optical fiber probe 41 can be reversely coupled and transmitted to the optical filter module 50; the probe array 40 is prepared by preparing four 105 μm/125 μm multimode fibers into a cone column combined structure by hydrofluoric acid chemical etching method, and then performing amino and aldehyde group biological activation and respectively coating to capture Hg 2+ The other section of report chain nucleic acid aptamer modified with quantum dots with different emission wavelengths can be combined with the capture chain nucleic acid aptamer on the probe under the action of the corresponding characteristic object to be detectedCombining to realize Hg according to fluorescence intensity signals of quantum dots of four channels 2+ Detecting the concentration of four substances, namely omethoate, microcystin-LR and escherichia coli; the filtering module 50 is composed of four bandpass filters, the passband wavelengths of which are consistent with the emission wavelengths of the four quantum dots, and is arranged at the output end of the signal light splitting device 30 and used for filtering the excitation light of the system and the stray light existing in the signal before imaging; the imaging system 60 mainly comprises a lens and a wireless black-and-white CMOS image sensor, can image four paths of fluorescent signals and display the four paths of fluorescent signals as four light spots, and the images shot by the image sensor are transmitted to a remote control and data display terminal 70 through wireless transmission to analyze and process the images; the control and data display terminal 70 can be realized by adopting raspberry pie, and the fluorescence intensity information of the quantum dots is obtained by calculating the gray value information of the four light spots, so that the concentration information of four objects to be detected is obtained, and the rapid, convenient and accurate detection and remote real-time monitoring of the above substances in the water environment can be realized.
The present invention has been described in detail with reference to the accompanying drawings, but the embodiments of the present invention are not limited to the above embodiments, and those skilled in the art can make various modifications to the present invention according to the prior art, which are all within the scope of the present invention.
Claims (7)
1. The multichannel optical fiber fluorescence sensor is characterized by comprising a light source with blue-violet wavelength, an excitation light splitting device connected with the light source, a signal light splitting device connected with the excitation light splitting device, a probe array and a light filtering device which are respectively connected with the signal light splitting device, an imaging system connected with the light filtering device and a control and data display terminal which are respectively connected with the light source and the imaging system in a communication way, wherein the probe array comprises more than two optical fiber probes, and the optical fiber probes are provided with a bare core area subjected to biological activation treatment, quantum dots connected with the bare core area and biological molecules used for identifying objects to be detected;
the light source, the excitation light splitting device and the signal light splitting device are detachably connected with the optical fiber probes through optical fiber connectors respectively;
the excitation light splitting device comprises a 1×n optical fiber coupler, a 1×n optical fiber beam splitter or a 1×n optical fiber wavelength division multiplexer, the signal light splitting device comprises N2×1 optical fiber couplers, 2×1 optical fiber loop devices or 2×1 optical fiber wavelength division multiplexers, wherein N is a natural number, N is greater than or equal to the number of the optical fiber probes, the input ends of the 1×n optical fiber couplers, the 1×n optical fiber beam splitters or the 1×n optical fiber wavelength division multiplexers are connected with the light source, N output ends are respectively connected with one first port of each 2×1 optical fiber coupler, each 2×1 optical fiber loop device or each 2×1 optical fiber wavelength division multiplexer, the other first port of each 2×1 optical fiber coupler, each 2×1 optical fiber loop device or each 2×1 optical fiber wavelength division multiplexer is respectively connected with the light filtering device, and the second port is respectively connected with each optical fiber probe one to one.
2. The multi-channel fiber optic fluorescence sensor according to claim 1, wherein said filtering means comprises two or more bandpass filters arranged in combination with each other.
3. The multi-channel fiber optic fluorescence sensor of claim 1, wherein the imaging system comprises a lens disposed at an outlet end of the light filtering device and a CMOS image sensor or a CCD image sensor mated with the lens, the CMOS image sensor or the CCD image sensor being in wireless communication with the control and data display terminal.
4. The multi-channel fiber optic fluorescence sensor of claim 1, wherein the light source is an LED light source, a semiconductor laser, or a solid state laser with an output pigtail that is a single mode or multimode fiber.
5. The multi-channel fiber optic fluorescence sensor according to any one of claims 1-4, further comprising a housing, wherein said light source, said excitation light splitting device, said signal light splitting device, said light filtering device, and said imaging system are enclosed within said housing, and wherein said control and data display terminal is enclosed within or located outside said housing.
6. The multi-channel fiber optic fluorescence sensor according to any one of claims 1-4, wherein said excitation light splitting means and said signal light splitting means are interconnected to form a fiber optic splitting means.
7. The multi-channel fiber optic fluorescence sensor according to any one of claims 1-4, wherein said control and data display terminal is a cell phone, tablet computer or microcomputer.
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| CN112326611B (en) * | 2020-10-29 | 2021-05-14 | 苏州雅睿生物技术有限公司 | N reagent hole M channel fluorescence detection method and design method |
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