CN110066734A - A kind of quickly breeding biological tropina culture medium - Google Patents
A kind of quickly breeding biological tropina culture medium Download PDFInfo
- Publication number
- CN110066734A CN110066734A CN201910417334.1A CN201910417334A CN110066734A CN 110066734 A CN110066734 A CN 110066734A CN 201910417334 A CN201910417334 A CN 201910417334A CN 110066734 A CN110066734 A CN 110066734A
- Authority
- CN
- China
- Prior art keywords
- parts
- component
- culture medium
- follows
- weight
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
A kind of quickly breeding biological tropina culture medium, it is related to microculture field, the component of the protein culture medium and the parts by weight of each component are as follows: 0.1-0.3 parts of growth factor, 20-25 parts of coagulator, 18-28 parts of protein additive, 0.5-1.5 parts of PH regulator, 1.3-2.9 parts of microelement, 9-15 parts of carbohydrate, 30-40 parts of water.The culture medium can promote microorganism itself for the absorption of nutriment and the progress of associated metabolic process, be conducive to that stablizing for microorganism is stimulated to grow and breed, furthermore, enough caloric intakes can be provided for microorganism by addition vitamin, protein additive etc., promote various microorganisms quickly to breed and be metabolized, compared with the existing technology in culture medium for, the culture medium culture microorganism speed in the present invention is fast and microbial reproduction is fast, nutrition is balanced, and the nutrition retention time is long.
Description
Technical field:
The present invention relates to microculture fields, and in particular to a kind of quickly breeding biological tropina culture medium.
Background technique:
Culture medium is the nutriment manually prepared for microorganism, plant tissue and animal tissue growth and maintenance, generally
All containing carbohydrate, nitrogen substance, inorganic salts (including microelement) and vitamin and water etc..Different culture medium can root
According to actual needs, some compounds that itself can not be synthesized, i.e. growth factor are added.Some microorganisms, such as autotrophic type microorganism,
Carbon source is not needed, so above-mentioned substance only has generality.Existing culture medium is divided into natural medium, synthetic media and half
Synthetic media, natural medium are made of natural materials, and the chemical component of this kind of culture medium is non-constant, it is also difficult to it determines,
The uncertainty of medium component is easy to cause the uncontrollability of incubation, and synthetic media is to have used various composition completely
The substance known configures, and the chemical component of this culture medium understands, repeatability is strong, easy to industrialized production, but microorganism
Growth in such culture medium is slower, and semisynthetic medium adds natural goods on the basis of being then utilized in synthetic media
Matter can not although culture speed has certain promotion, and culture speed is still relatively slow for synthetic media
Meets the needs of quickly producing, the microorganism speed of growth is slow, needs to consume the more time so as to cause microbial reproduction, extends
The duration of microculture and the entire process of subsequent production processing.
Summary of the invention:
In place of overcoming above-mentioned the shortcomings of the prior art, and provide a kind of quickly breeding life
Object mycoprotein culture medium.
The technical solution adopted by the present invention are as follows: a kind of quickly breeding biological tropina culture medium, the protein culture medium
The parts by weight of component and each component are as follows: 0.1-0.3 parts of growth factor, 20-25 parts of coagulator, 18-28 parts of protein additive, PH
0.5-1.5 parts of regulator, 1.3-2.9 parts of microelement, 9-15 parts of carbohydrate, 30-40 parts of water.
The component of the growth factor and the parts by weight of each component are as follows: 3-7 parts of cow's serum, 0.5-1.7 parts of insulin,
0.7-1.3 parts of sterol, 1-2 parts of porphyrin, 0.01-0.5 parts of fibroblast growth factor, 3-4 parts of rose hip oil, cut melon extract
10-15 parts.
The component of the protein additive and the parts by weight of each component are as follows: 18-29 parts of peptone, 5-10 parts of fish glue,
7-10 parts of collagen, 3-8 parts of amino acid, 3-10 parts of bean dregs, 4-8 parts of brewex's grains, 10-12 parts of Yeast protein.
The component of the coagulator and the parts by weight of each component are as follows: 10-15 parts of agar, 17-21 parts of xanthan gum, pectin
7-10 parts, 2-11 parts of dehydrated potato powder, 9-10 parts of egg white, 4-8 parts of citric acid molysite, 2-7 parts of glucolactone, viscose 3-
10 parts.
The component of the microelement and the parts by weight of each component are as follows: 3-9 parts of tocopherol acetate, ferrous sulfate 9-
12 parts, 2-5 parts of potassium citrate, 1-4 parts of sodium chloride, 2-4 parts of sodium citrate, 3-9 parts of ammonium dihydrogen phosphate, 1-4 parts of calcium carbonate, carbonic acid
1-4 parts of magnesium, 0.1-0.7 parts of potassium nitrite.
The component of the carbohydrate and the parts by weight of each component are as follows: 8-10 parts of Extraction of Eucheuma gelatinae, 3-6 parts of honey, sea
1-4 parts of mosanom, 2-7 parts of liquiritin, 9-12 parts of sweet potato mash, 1-2 parts of chitosan oligosaccharide, 10-15 parts of ammonium chloride, ammonium molybdate 0.5-1.8
Part, 0.5-1 parts of sodium selenite, 9-10 parts of polysaccharides.
The component of the PH regulator and the parts by weight of each component are as follows: 0.01-0.1 parts of borax, reindeer moss indicator solution 1-3
Part, 7-10 parts of buffer, 1-2 parts of butyl acetate, 2-6 parts of Pfansteihl.
The component of the buffer and the parts by weight of each component are as follows: 3-7 parts of ammonium hydroxide, 1-2 parts of sodium hydroxide, Strong oxdiative
0.2-0.7 parts of calcium, 3-9 parts of potassium acetate.
The component of the buffer and the parts by weight of each component are as follows: 3-7 parts of ammonium chloride, 3-4 parts of acetic acid, hydrochloric acid 1-4
Part.
The invention has the benefit that the culture medium can promote absorption and phase of the microorganism itself for nutriment
The progress for closing metabolic process is conducive to that stablizing for microorganism is stimulated to grow and breed, in addition, adding by addition vitamin, albumen
Add agent etc. that can provide enough caloric intakes for microorganism, promotes various microorganisms quickly to breed and be metabolized, relative to existing
For culture medium in technology, the culture medium culture microorganism speed in the present invention is fast and microbial reproduction is fast, and nutrition is balanced, battalion
It is long to support the retention time.
Specific embodiment:
Invention is further explained in conjunction with specific embodiments:
A kind of quickly breeding biological tropina culture medium, the component of the protein culture medium and the parts by weight of each component
Are as follows: 0.1-0.3 parts of growth factor, 20-25 parts of coagulator, 18-28 parts of protein additive, 0.5-1.5 parts of PH regulator, micro member
It is 1.3-2.9 parts plain, 9-15 parts of carbohydrate, 30-40 parts of water.Also added with compound catabolic enzyme in the culture medium: 3-7 parts of acetyl coenzyme A,
- 6 parts of β-galactosidase 1,8-12 parts of cellulase, 2-7 parts of chymotrypsin, 6-9 parts of subtilopeptidase A, leucylamino
1-3 parts of peptase, 4-8 parts of xylanase clastic enzyme, 0.5-2.5 parts of chitosan catabolic enzyme, 3-8 parts of pectin hydrolase, mannosan point
Enzyme 7-11 parts of solution.
The component of the growth factor and the parts by weight of each component are as follows: 3-7 parts of cow's serum, 0.5-1.7 parts of insulin,
0.7-1.3 parts of sterol, 1-2 parts of porphyrin, 0.01-0.5 parts of fibroblast growth factor, 3-4 parts of rose hip oil, cut melon extract
10-15 parts.
The component of the protein additive and the parts by weight of each component are as follows: 18-29 parts of peptone, 5-10 parts of fish glue,
7-10 parts of collagen, 3-8 parts of amino acid, 3-10 parts of bean dregs, 4-8 parts of brewex's grains, 10-12 parts of Yeast protein.
The component of the coagulator and the parts by weight of each component are as follows: 10-15 parts of agar, 17-21 parts of xanthan gum, pectin
7-10 parts, 2-11 parts of dehydrated potato powder, 9-10 parts of egg white, 4-8 parts of citric acid molysite, 2-7 parts of glucolactone, viscose 3-
10 parts.
The component of the microelement and the parts by weight of each component are as follows: 3-9 parts of tocopherol acetate, ferrous sulfate 9-
12 parts, 2-5 parts of potassium citrate, 1-4 parts of sodium chloride, 2-4 parts of sodium citrate, 3-9 parts of ammonium dihydrogen phosphate, 1-4 parts of calcium carbonate, carbonic acid
1-4 parts of magnesium, 0.1-0.7 parts of potassium nitrite.
The component of the carbohydrate and the parts by weight of each component are as follows: 8-10 parts of Extraction of Eucheuma gelatinae, 3-6 parts of honey, sea
1-4 parts of mosanom, 2-7 parts of liquiritin, 9-12 parts of sweet potato mash, 1-2 parts of chitosan oligosaccharide, 10-15 parts of ammonium chloride, ammonium molybdate 0.5-1.8
Part, 0.5-1 parts of sodium selenite, 9-10 parts of polysaccharides.
The component of the PH regulator and the parts by weight of each component are as follows: 0.01-0.1 parts of borax, reindeer moss indicator solution 1-3
Part, 7-10 parts of buffer, 1-2 parts of butyl acetate, 2-6 parts of Pfansteihl.
The component of the buffer and the parts by weight of each component are as follows: 3-7 parts of ammonium hydroxide, 1-2 parts of sodium hydroxide, Strong oxdiative
0.2-0.7 parts of calcium, 3-9 parts of potassium acetate.
The component of the buffer and the parts by weight of each component are as follows: 3-7 parts of ammonium chloride, 3-4 parts of acetic acid, hydrochloric acid 1-4
Part.
Embodiment one:
A kind of quickly breeding biological tropina culture medium, the component of the protein culture medium and the parts by weight of each component
Are as follows: 0.1 part of growth factor, 20 parts of coagulator, 18 parts of protein additive, 0.5 part of PH regulator, 1.3 parts of microelement, carbohydrate 9
Part, 30 parts of water.Also added with compound catabolic enzyme in the culture medium: 3 parts of acetyl coenzyme A, β-galactosidase 1 part, cellulase 8
Part, 2 parts of chymotrypsin, 6 parts of subtilopeptidase A, 1 part of leucyl aminopeptidases, 4 parts of xylanase clastic enzyme, chitosan decomposes
0.5 part of enzyme, 3 parts of pectin hydrolase, 7 parts of mannosan catabolic enzyme.
The component of the growth factor and the parts by weight of each component are as follows: 3 parts of cow's serum, 0.5 part of insulin, sterol
0.7 part, 1 part of porphyrin, 0.01 part of fibroblast growth factor, 3 parts of rose hip oil, cut 10 parts of melon extract.
The component of the protein additive and the parts by weight of each component are as follows: 18 parts of peptone, 5 parts of fish glue, collagen egg
White 7 parts, 3 parts of amino acid, 3 parts of bean dregs, 4 parts of brewex's grains, 10 parts of Yeast protein.
The component of the coagulator and the parts by weight of each component are as follows: 10 parts of agar, 17 parts of xanthan gum, 7 parts of pectin, horse
2 parts of bell potato powder, 9 parts of egg white, 4 parts of citric acid molysite, 2 parts of glucolactone, 3 parts of viscose.
The component of the microelement and the parts by weight of each component are as follows: 3 parts of tocopherol acetate, 9 parts of ferrous sulfate,
2 parts of potassium citrate, 1 part of sodium chloride, 2 parts of sodium citrate, 3 parts of ammonium dihydrogen phosphate, 1 part of calcium carbonate, 1 part of magnesium carbonate, potassium nitrite
0.1 part.
The component of the carbohydrate and the parts by weight of each component are as follows: 8 parts of Extraction of Eucheuma gelatinae, 3 parts of honey, sodium alginate
1 part, 2 parts of liquiritin, 9 parts of sweet potato mash, 1 part of chitosan oligosaccharide, 10 parts of ammonium chloride, 0.5 part of ammonium molybdate, 0.5 part of sodium selenite, fructus lycii is more
9 parts of sugar.
The component of the PH regulator and the parts by weight of each component are as follows: 0.01 part of borax, 1 part of reindeer moss indicator solution, delay
7 parts of fliud flushing, 1 part of butyl acetate, 2 parts of Pfansteihl.
The component of the buffer and the parts by weight of each component are as follows: 3 parts of ammonium hydroxide, 1 part of sodium hydroxide, Strong oxdiative calcium
0.2 part, 3 parts of potassium acetate.
Embodiment two:
A kind of quickly breeding biological tropina culture medium, the component of the protein culture medium and the parts by weight of each component
Are as follows: 0.3 part of growth factor, 25 parts of coagulator, 28 parts of protein additive, 1.5 parts of PH regulator, 2.9 parts of microelement, carbohydrate 15
Part, 40 parts of water.Also added with compound catabolic enzyme in the culture medium: 7 parts of acetyl coenzyme A, 6 parts of beta galactosidase, cellulase
12 parts, 7 parts of chymotrypsin, 9 parts of subtilopeptidase A, 3 parts of leucyl aminopeptidases, 8 parts of xylanase clastic enzyme, chitosan point
2.5 parts of enzyme, 8 parts of pectin hydrolase, 11 parts of mannosan catabolic enzyme of solution.
The component of the growth factor and the parts by weight of each component are as follows: 7 parts of cow's serum, 1.7 parts of insulin, sterol
1.3 parts, 2 parts of porphyrin, 0.5 part of fibroblast growth factor, 4 parts of rose hip oil, cut 15 parts of melon extract.
The component of the protein additive and the parts by weight of each component are as follows: 29 parts of peptone, 10 parts of fish glue, collagen egg
White 10 parts, 8 parts of amino acid, 10 parts of bean dregs, 8 parts of brewex's grains, 12 parts of Yeast protein.
The component of the coagulator and the parts by weight of each component are as follows: 15 parts of agar, 21 parts of xanthan gum, 10 parts of pectin,
11 parts of dehydrated potato powder, 10 parts of egg white, 8 parts of citric acid molysite, 7 parts of glucolactone, 10 parts of viscose.
The component of the microelement and the parts by weight of each component are as follows: 9 parts of tocopherol acetate, ferrous sulfate 12
Part, 5 parts of potassium citrate, 4 parts of sodium chloride, 4 parts of sodium citrate, 9 parts of ammonium dihydrogen phosphate, 4 parts of calcium carbonate, 4 parts of magnesium carbonate, nitrous acid
0.7 part of potassium.
The component of the carbohydrate and the parts by weight of each component are as follows: 10 parts of Extraction of Eucheuma gelatinae, 6 parts of honey, alginic acid
4 parts of sodium, 7 parts of liquiritin, 12 parts of sweet potato mash, 2 parts of chitosan oligosaccharide, 15 parts of ammonium chloride, 1.8 parts of ammonium molybdate, 1 part of sodium selenite, fructus lycii
10 parts of polysaccharide.
The component of the PH regulator and the parts by weight of each component are as follows: 0.1 part of borax, 3 parts of reindeer moss indicator solution, buffering
10 parts of liquid, 2 parts of butyl acetate, 6 parts of Pfansteihl.
The component of the buffer and the parts by weight of each component are as follows: 7 parts of ammonium hydroxide, 2 parts of sodium hydroxide, Strong oxdiative calcium
0.7 part, 9 parts of potassium acetate.
The component of the buffer and the parts by weight of each component are as follows: 7 parts of ammonium chloride, 4 parts of acetic acid, 4 parts of hydrochloric acid.
Embodiment three:
A kind of quickly breeding biological tropina culture medium, the component of the protein culture medium and the parts by weight of each component
Are as follows: 0.2 part of growth factor, 22 parts of coagulator, 25 parts of protein additive, 1.1 parts of PH regulator, 2.4 parts of microelement, carbohydrate 13
Part, 35 parts of water.Also added with compound catabolic enzyme in the culture medium: 5 parts of acetyl coenzyme A, 5 parts of beta galactosidase, cellulase
10 parts, 5 parts of chymotrypsin, 7 parts of subtilopeptidase A, 2 parts of leucyl aminopeptidases, 6 parts of xylanase clastic enzyme, chitosan point
2.2 parts of enzyme, 4 parts of pectin hydrolase, 10 parts of mannosan catabolic enzyme of solution.
The component of the growth factor and the parts by weight of each component are as follows: 5 parts of cow's serum, 1.3 parts of insulin, sterol
1.1 parts, 1.5 parts of porphyrin, 0.04 part of fibroblast growth factor, 3.5 parts of rose hip oil, cut 12 parts of melon extract.
The component of the protein additive and the parts by weight of each component are as follows: 22 parts of peptone, 7 parts of fish glue, collagen egg
White 8 parts, 6 parts of amino acid, 7 parts of bean dregs, 5 parts of brewex's grains, 11 parts of Yeast protein.
The component of the coagulator and the parts by weight of each component are as follows: 12 parts of agar, 18 parts of xanthan gum, 8 parts of pectin, horse
7 parts of bell potato powder, 10 parts of egg white, 6 parts of citric acid molysite, 5 parts of glucolactone, 7 parts of viscose.
The component of the microelement and the parts by weight of each component are as follows: 7 parts of tocopherol acetate, ferrous sulfate 10
Part, 4 parts of potassium citrate, 3 parts of sodium chloride, 3 parts of sodium citrate, 6 parts of ammonium dihydrogen phosphate, 3 parts of calcium carbonate, 3 parts of magnesium carbonate, nitrous acid
0.5 part of potassium.
The component of the carbohydrate and the parts by weight of each component are as follows: 9 parts of Extraction of Eucheuma gelatinae, 5 parts of honey, sodium alginate
3 parts, 5 parts of liquiritin, 11 parts of sweet potato mash, 1.5 parts of chitosan oligosaccharide, 12 parts of ammonium chloride, 1.5 parts of ammonium molybdate, 0.7 part of sodium selenite, Chinese holly
9.5 parts of Qi polysaccharide.
The component of the PH regulator and the parts by weight of each component are as follows: 0.05 part of borax, 2 parts of reindeer moss indicator solution, delay
8 parts of fliud flushing, 1.5 parts of butyl acetate, 3 parts of Pfansteihl.
The component of the buffer and the parts by weight of each component are as follows: 5 parts of ammonium hydroxide, 1.5 parts of sodium hydroxide, Strong oxdiative calcium
0.5 part, 5 parts of potassium acetate.
Example IV:
A kind of quickly breeding biological tropina culture medium, the component of the protein culture medium and the parts by weight of each component
Are as follows: 0.2 part of growth factor, 24 parts of coagulator, 27 parts of protein additive, 1.3 parts of PH regulator, 2.7 parts of microelement, carbohydrate 12
Part, 37 parts of water.
The component of the growth factor and the parts by weight of each component are as follows: 6 parts of cow's serum, 1.5 parts of insulin, sterol
1.2 parts, 1.5 parts of porphyrin, 0.07 part of fibroblast growth factor, 3.7 parts of rose hip oil, cut 12 parts of melon extract.
The component of the protein additive and the parts by weight of each component are as follows: 24 parts of peptone, 7 parts of fish glue, collagen egg
White 8 parts, 6 parts of amino acid, 7 parts of bean dregs, 5 parts of brewex's grains, 11 parts of Yeast protein.
The component of the coagulator and the parts by weight of each component are as follows: 12 parts of agar, 20 parts of xanthan gum, 8 parts of pectin, horse
9 parts of bell potato powder, 9.4 parts of egg white, 6 parts of citric acid molysite, 5 parts of glucolactone, 8 parts of viscose.
The component of the microelement and the parts by weight of each component are as follows: 6 parts of tocopherol acetate, ferrous sulfate 11
Part, 3 parts of potassium citrate, 2.5 parts of sodium chloride, 3 parts of sodium citrate, 4 parts of ammonium dihydrogen phosphate, 3 parts of calcium carbonate, 3 parts of magnesium carbonate, nitrous
0.4 part of sour potassium.
The component of the carbohydrate and the parts by weight of each component are as follows: 9 parts of Extraction of Eucheuma gelatinae, 4 parts of honey, sodium alginate
3 parts, 4 parts of liquiritin, 10 parts of sweet potato mash, 1.5 parts of chitosan oligosaccharide, 12 parts of ammonium chloride, 1.3 parts of ammonium molybdate, 0.7 part of sodium selenite, Chinese holly
9.7 parts of Qi polysaccharide.
The component of the PH regulator and the parts by weight of each component are as follows: 0.07 part of borax, 1.4 parts of reindeer moss indicator solution,
8 parts of buffer, 1.2 parts of butyl acetate, 2.5 parts of Pfansteihl.
The component of the buffer and the parts by weight of each component are as follows: 4.7 parts of ammonium chloride, 5.6 parts of acetic acid, hydrochloric acid 3.2
Part.
The culture medium is used for all raw materials to be proportionally added in agitated kettle and is stirred, and is warming up to 30 DEG C -50 DEG C,
Make all dissolution of raw material and after mixing, is cooled to room temperature.
The type for the microorganism that acid buffer agent is cultivated as needed with ealkaline buffer is selected.
The configuration method that culture medium of the embodiment one into example IV is all made of conventional culture medium is configured.
Using effect experiment:
This research is examined by a kind of using effect of 100 enterprises to quickly breeding biological tropina culture medium
It examines, test item standards of grading are as follows: very satisfied 5 points, be satisfied with 4 points, general 3 points, be unsatisfied with 2 points, very dissatisfied 1 point.Its
In common group using common culture medium；
| Test item | Embodiment one | Embodiment two | Embodiment three | Example IV | Control group |
| Culture effect | 5.0 | 5.0 | 5.0 | 4.6 | 3.0 |
| Cultivate speed | 4.8 | 4.9 | 5.0 | 4.7 | 2.0 |
| Safety | 5.0 | 4.9 | 5.0 | 4.8 | 3.0 |
| Environmental protection | 4.7 | 4.9 | 4.6 | 4.7 | 3.2 |
Culture experiment:
Culture experiment is divided into control group and test group, and control group uses beef-protein medium, and totally four groups of test group,
Protein culture medium of the embodiment one into example IV is respectively adopted in test group 1 to test group 4, respectively takes in control group and test group
40g is simultaneously divided into 4 parts and is contained in 4 culture mediums, cultivates nitrogen-fixing bacteria, saccharomycete, cream respectively to four culture mediums in each group
Sour bacterium, Actinobacillus, record growth situation (measure the 0D of bacterial strain after 5h and 10h600Value), concrete outcome is as follows
Described in table:
By the way that compared with the control group, test group can be tried with fast culture Actinobacillus, nitrogen-fixing bacteria, saccharomycete and lactic acid bacteria
Testing group can promote microorganism to grow, beneficial to microculture, in addition, after adding compound catabolic enzyme in formula, micro- life
The growing state of object becomes more preferably, and the speed of microbial reproduction is speeded.
In conclusion the culture medium can promote absorption and associated metabolic process of the microorganism itself for nutriment
Progress, be conducive to stimulate microorganism stablizes growth and breeding, in addition, can be with by addition vitamin, protein additive etc.
There is provided enough caloric intakes for microorganism, various microorganisms promoted quickly to breed and be metabolized, compared with the existing technology in training
For supporting base, the culture medium culture microorganism speed in the present invention is fast and microbial reproduction is fast, and nutrition is balanced, the nutrition retention time
Long.
For those skilled in the art, it is various corresponding that other can be made according to above technical scheme and design
Change and deformation, and all these change and modification all should belong within the scope of protection of the claims of the present invention.
Claims (9)
1. a kind of quickly breeding biological tropina culture medium, it is characterised in that: the component of the protein culture medium and each component
Parts by weight are as follows: 0.1-0.3 parts of growth factor, 20-25 parts of coagulator, 18-28 parts of protein additive, PH regulator 0.5-1.5
Part, 1.3-2.9 parts of microelement, 9-15 parts of carbohydrate, 30-40 parts of water.
2. quick breeding biological tropina culture medium according to claim 1, it is characterised in that: the growth factor
Component and each component parts by weight are as follows: 3-7 parts of cow's serum, 0.5-1.7 parts of insulin, 0.7-1.3 parts of sterol, porphyrin 1-2
Part, 0.01-0.5 parts of fibroblast growth factor, 3-4 parts of rose hip oil, cut 10-15 parts of melon extract.
3. quick breeding biological tropina culture medium according to claim 1, it is characterised in that: the albumen addition
The component of agent and the parts by weight of each component are as follows: 18-29 parts of peptone, 5-10 parts of fish glue, 7-10 parts of collagen, amino acid 3-
8 parts, 3-10 parts of bean dregs, 4-8 parts of brewex's grains, 10-12 parts of Yeast protein.
4. quick breeding biological tropina culture medium according to claim 1, it is characterised in that: the coagulator
The parts by weight of component and each component are as follows: 10-15 parts of agar, 17-21 parts of xanthan gum, 7-10 parts of pectin, 2-11 parts of dehydrated potato powder,
9-10 parts of egg white, 4-8 parts of citric acid molysite, 2-7 parts of glucolactone, 3-10 parts of viscose.
5. quick breeding biological tropina culture medium according to claim 1, it is characterised in that: the microelement
Component and each component parts by weight are as follows: 3-9 parts of tocopherol acetate, 9-12 parts of ferrous sulfate, 2-5 parts of potassium citrate, chlorine
Sodium 1-4 parts of change, 2-4 parts of sodium citrate, 3-9 parts of ammonium dihydrogen phosphate, 1-4 parts of calcium carbonate, 1-4 parts of magnesium carbonate, potassium nitrite 0.1-
0.7 part.
6. quick breeding biological tropina culture medium according to claim 1, it is characterised in that: the group of the carbohydrate
The parts by weight of part and each component are as follows: 8-10 parts of Extraction of Eucheuma gelatinae, 3-6 parts of honey, 1-4 parts of sodium alginate, liquiritin 2-7
Part, 9-12 parts of sweet potato mash, 1-2 parts of chitosan oligosaccharide, 10-15 parts of ammonium chloride, 0.5-1.8 parts of ammonium molybdate, 0.5-1 parts of sodium selenite, Chinese holly
9-10 parts of Qi polysaccharide.
7. quick breeding biological tropina culture medium according to claim 1, it is characterised in that: the PH regulator
Component and each component parts by weight are as follows: 0.01-0.1 parts of borax, 1-3 parts of reindeer moss indicator solution, 7-10 parts of buffer, acetic acid fourth
1-2 parts of ester, 2-6 parts of Pfansteihl.
8. quick breeding biological tropina culture medium according to claim 7, it is characterised in that: the buffer
The parts by weight of component and each component are as follows: 3-7 parts of ammonium hydroxide, 1-2 parts of sodium hydroxide, 0.2-0.7 parts of Strong oxdiative calcium, potassium acetate 3-9
Part.
9. quick breeding biological tropina culture medium according to claim 7, it is characterised in that: the buffer
The parts by weight of component and each component are as follows: 3-7 parts of ammonium chloride, 3-4 parts of acetic acid, 1-4 parts of hydrochloric acid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910417334.1A CN110066734A (en) | 2019-05-20 | 2019-05-20 | A kind of quickly breeding biological tropina culture medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910417334.1A CN110066734A (en) | 2019-05-20 | 2019-05-20 | A kind of quickly breeding biological tropina culture medium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110066734A true CN110066734A (en) | 2019-07-30 |
Family
ID=67370976
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910417334.1A Pending CN110066734A (en) | 2019-05-20 | 2019-05-20 | A kind of quickly breeding biological tropina culture medium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110066734A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112680499A (en) * | 2021-01-27 | 2021-04-20 | 浙江夸克生物科技有限公司 | In-vitro detection kit for anaerobic microorganisms |
| CN112831539A (en) * | 2021-01-27 | 2021-05-25 | 浙江夸克生物科技有限公司 | In-vitro detection kit for aerobic microorganisms |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| UA63209A (en) * | 2003-02-28 | 2004-01-15 | Ivan Nykyforovych Bezkopylnyi | A method for producing the solid nutrient medium for cultivation of micobacteria |
| CN106434492A (en) * | 2016-11-29 | 2017-02-22 | 齐鲁工业大学 | Culture medium for effectively prolonging survival time of lactic acid bacteria and preparation method of culture medium |
| CN107858320A (en) * | 2017-11-30 | 2018-03-30 | 浙江同创越诚健康科技有限公司 | Bacteria culture media, cultural method and the application of the two |
| CN108060083A (en) * | 2017-12-23 | 2018-05-22 | 安徽睿知信信息科技有限公司 | A kind of agricultural microbiological culture media and its production method |
| CN109161508A (en) * | 2018-09-30 | 2019-01-08 | 上海景峰制药有限公司 | A kind of microbiological culture media and the preparation method and application thereof |
-
2019
- 2019-05-20 CN CN201910417334.1A patent/CN110066734A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| UA63209A (en) * | 2003-02-28 | 2004-01-15 | Ivan Nykyforovych Bezkopylnyi | A method for producing the solid nutrient medium for cultivation of micobacteria |
| CN106434492A (en) * | 2016-11-29 | 2017-02-22 | 齐鲁工业大学 | Culture medium for effectively prolonging survival time of lactic acid bacteria and preparation method of culture medium |
| CN107858320A (en) * | 2017-11-30 | 2018-03-30 | 浙江同创越诚健康科技有限公司 | Bacteria culture media, cultural method and the application of the two |
| CN108060083A (en) * | 2017-12-23 | 2018-05-22 | 安徽睿知信信息科技有限公司 | A kind of agricultural microbiological culture media and its production method |
| CN109161508A (en) * | 2018-09-30 | 2019-01-08 | 上海景峰制药有限公司 | A kind of microbiological culture media and the preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 傅月华等: "真菌培养基营养成分原理分析", 《彭城大学学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112680499A (en) * | 2021-01-27 | 2021-04-20 | 浙江夸克生物科技有限公司 | In-vitro detection kit for anaerobic microorganisms |
| CN112831539A (en) * | 2021-01-27 | 2021-05-25 | 浙江夸克生物科技有限公司 | In-vitro detection kit for aerobic microorganisms |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106858066A (en) | Collaboration promotes the additive and application method of proliferation of intestinal probiotics and field planting | |
| CN105018544B (en) | With the carrageenan oligosaccharide and its preparation method and application for promoting sea cucumber growth and degeneration-resistant effect | |
| CN103756935B (en) | Bifidobacterium and lactobacillus mixed fermentation medium and fermentation method | |
| CN102311927A (en) | Medium and method for high density fermentation of saccharomyces cerevisiae | |
| CN110066734A (en) | A kind of quickly breeding biological tropina culture medium | |
| CN103131652B (en) | Rhizobium japonicum culture medium and method for preparing liquid rhizobium japonicum agent by adopting rhizobium japonicum culture medium | |
| CN110982750A (en) | High-density fermentation method for rhodopseudomonas palustris and application of high-density fermentation method | |
| CN104585510A (en) | Feed additive containing mixed bacterium fermented product and preparation method for feed additive | |
| CN105483014A (en) | Production technology for high-density culture of chlorella by utilizing fermentation method | |
| CN108991244A (en) | A kind of additive agent for feeding that animal body digestic property can be improved and production technology | |
| CN101275154B (en) | Production method for microbial polysaccharide welan gum | |
| CN1579197A (en) | Bacterisugar mono-cell protein feed producing method | |
| CN104894028A (en) | Fishery ocean microbial ecological preparation and preparation method thereof | |
| CN108587966B (en) | Pure natural plant source EM (effective microorganisms) culture medium and application of fermentation product thereof in aquaculture | |
| CN102326673B (en) | Method for producing aerobic fermentation photosynthetic bacteria livestock culturing feed additive | |
| CN101338293B (en) | Method for producing microecological preparation of bdellovibrio bacteriourus | |
| CN103844055A (en) | Microecologic feed additive for deer and preparation method of feed additive | |
| US20080213826A1 (en) | Compound microbial preparation manufacturing process | |
| CN114304387A (en) | Protein mulberry leavening agent and preparation method and application thereof | |
| CN106754522A (en) | A kind of preparation method of bacillus subtilis fermentation liquor | |
| CN104450599A (en) | Probiotic corn leavening agent capable of increasing content of L-lactic acid and application of probiotic corn leavening agent | |
| CN112369528A (en) | Microbial preparation for improving intestinal environment of poultry and preparation method thereof | |
| CN110055190A (en) | A kind of cultural method of clostridium septicum | |
| CN105296407A (en) | Method for culturing avibacterium paragallinarum bacterial solution | |
| CN110150505A (en) | A kind of lactic acid bacteria complexing agent and preparation method thereof removing Eriocheir sinensis bilgy odour |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190730 |
|
| RJ01 | Rejection of invention patent application after publication |