CN110051590A - Whitening, spot composition and cosmetics containing the composition - Google Patents
Whitening, spot composition and cosmetics containing the composition Download PDFInfo
- Publication number
- CN110051590A CN110051590A CN201910429141.8A CN201910429141A CN110051590A CN 110051590 A CN110051590 A CN 110051590A CN 201910429141 A CN201910429141 A CN 201910429141A CN 110051590 A CN110051590 A CN 110051590A
- Authority
- CN
- China
- Prior art keywords
- parts
- whitening
- spot
- fermentation
- composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Botany (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Dermatology (AREA)
- Cosmetics (AREA)
Abstract
The present invention relates to cosmetic technical fields, in particular to Whitening, spot composition and the cosmetics containing the composition.The composition is formed by the fermenting raw materials of following parts by weight: 5-15 parts of Rhizoma Chuanxiong, 6-10 parts of red pomegranate, 2-5 parts of cordate houttuynia, 15-20 parts of kuh-seng, turning round cactus 10-18 parts and purple coneflower 5-8 parts of thorn.The present invention extracts the active nutrients of more horn of plenty and more small molecule using fermentation technique from the plant that six kinds of green naturals of cactus and purple coneflower are pierced in Rhizoma Chuanxiong, red pomegranate, cordate houttuynia, kuh-seng, torsion, it enhances plant material effect, reduce toxic side effect, safety greatly improves.Experiment shows that the present composition has the effect that significantly inhibits to tyrosinase, and cosmetics obtained have stronger inhibiting effect to dermal melanin content, can be obviously improved skin brightness, has Whitening, spot effect outstanding.
Description
Technical field
The present invention relates to cosmetic technical field more particularly to Whitening, spot composition and contain the makeup of the composition
Product.
Background technique
Color spot is always for a long time the big problem for perplexing human skin, and in particular with the growth at age, skin goes out
The probability of existing color spot also obviously increases, and world cosmetics expert has been devoted to develop various whitening spot-removing class products.
Currently on the market common whitening spot-removing ingredient include arbutin and its derivative, hydroquinone derivatives, kojic acid and its
Derivative, tartaric acid class, vitamin C and its derivative, nicotinamide and some plant extracts with whitening effect
Ingredient etc..Wherein, although arbutin and its ingredients effect such as derivative, hydroquinone derivatives, kojic acid and its derivative, tartaric acid class compared with
It is good, but cytotoxicity and irritation are relatively large, and the normal physiological metabolism for being easy to interfere skin is used for a long time, causes
Some undesirable side effects, such as reduce the photoprotection of skin, easy rebound, skin allergy, contact dermatitis.Such as invention
A kind of whitening spot-removing skin care item of patent and preparation method thereof (2018100064410) disclose one kind with arbutin, ascorbic acid
Four kinds of polypeptide, niacinamide and Symwhite-337 whitening agents are used in compounding, and combine other functional components, make skin care item can be from
Bright color, color spot of dispelling, the ultraviolet class that dispels, functions of removing chloasma, five aspects of red sector of dispelling improve skin condition, effective freckle removing and whitening.Such as
Patent of invention discloses a kind of whitening spot-removing Essence (201810693370.6), in reasonable employment Symwhite-337, cigarette
On the basis of the whitening spot-removings ingredient such as amide, magnesium ascorbyl phosphate, musk rose seed is added in whitening spot-removing Essence
Oil, Prunus Amygdalus Dulcis Oil, glycyrrhiza glabra root extract, three, which is used in compounding, significantly improves whitening composition in whitening spot-removing Essence
Whitening spot-removing effect.
With the raising of living standards of the people and aesthetical standard, pale and clean skin is even more increasingly to be praised highly.People
Requirement to cosmetic industry it is higher and higher, the natural cosmetics of regress into nature are increasingly yearned for and people's environmental consciousness
Raising, people start to focus on natural, safe and healthy and environmentally friendly theory, therefore, need to develop a kind of natural safety, and energy
Meets the whitening and freckle-removing product of the needs of people.
Summary of the invention
In view of this, a kind of the purpose of the present invention is to provide Whitening, spot effects good, highly-safe Whitening, spot group
Close object.
In order to achieve the object of the present invention, the present invention adopts the following technical scheme:
A kind of Whitening, spot composition, is formed by the fermenting raw materials of following parts by weight:
5-15 parts of Rhizoma Chuanxiong, 6-10 parts of red pomegranate, 2-5 parts of cordate houttuynia, 15-20 parts of kuh-seng, turn round cactus 10-18 parts of thorn and purple
5-8 parts of Echinacea purpurea.
In some specific embodiments, the Whitening, spot composition is formed by the fermenting raw materials of following parts by weight:
5 parts of Rhizoma Chuanxiong, 6 parts of red pomegranate, 2 parts of cordate houttuynia, 15 parts of kuh-seng, turn round 10 parts and 5 parts of purple coneflower of thorn cactus.
In some specific embodiments, the Whitening, spot composition is formed by the fermenting raw materials of following parts by weight:
8 parts of Rhizoma Chuanxiong, 7 parts of red pomegranate, 3 parts of cordate houttuynia, 16 parts of kuh-seng, turn round 12 parts and 5 parts of purple coneflower of thorn cactus.
The present invention pierces cactus and six kinds of purple coneflower from Rhizoma Chuanxiong, red pomegranate, cordate houttuynia, kuh-seng, torsion using fermentation technique
The active nutrients that more horn of plenty and more small molecule are extracted in the plant of green natural enhance plant material effect, reduce
Toxic side effect, safety greatly improve.Experiment shows that the present composition has tyrosinase and significantly inhibits effect, makes
The cosmetics obtained have stronger inhibiting effect to dermal melanin content, can be obviously improved skin brightness, have outstanding
Whitening, spot effect.
In Whitening, spot composition provided by the invention, the strain of the fermentation is Bacillus acidi lactici, saccharomycete and root nodule
Bacterium it is one or more.
In some embodiments, the strain of the fermentation includes Bacillus acidi lactici, saccharomycete and rhizobium.The lactic acid bar
The weight ratio of bacterium, saccharomycete and rhizobium is preferably 2:2:1.
The present invention also provides the preparation methods of the Whitening, spot composition, comprising:
By Rhizoma Chuanxiong, red pomegranate, cordate houttuynia, kuh-seng, turns round thorn cactus and after purple coneflower crushes, mixes, water is added to carry out the
One fermentation, obtains the first fermentation liquid；
By the first filtering fermentation liquor, filtrate is inoculated with strain after high-temperature sterilization and carries out second of fermentation, obtains the second fermentation liquid；
Second fermentation liquid is inactivated, filtering obtains the Whitening, spot composition.
In some embodiments, the strain is the one or more of Bacillus acidi lactici, saccharomycete and rhizobium.It is some specific
In embodiment, strain includes Bacillus acidi lactici, saccharomycete and rhizobium；Preferably, the inoculum concentration of the strain is 105-108CFU/
ml。
In some embodiments, first fermentation carries out in herb fermenting tank, and the temperature of fermentation is 121 DEG C, fermentation
Time is 90min.
After obtaining the first fermentation liquid, by first filtering fermentation liquor, filtrate high-temperature sterilization is taken.In some embodiments,
The temperature of high-temperature sterilization is 121 DEG C.
It is inoculated with strain to the filtrate after the high pressure sterilization, carries out the second fermentation.In some embodiments, the second fermentation is
At 28-37 DEG C of temperature, pH value 4-6.5, revolving speed 100-250r/min, dissolved oxygen amount carries out under conditions of being 100-150%, sends out
The ferment time is 48-72h.Before being inoculated with strain, first strain is activated, being made into concentration is 105-108The bacterium of CFU/ml
Liquid.In some embodiments, the strain is the one or more of Bacillus acidi lactici, saccharomycete and rhizobium.Some specific implementations
In scheme, the strain includes Bacillus acidi lactici, saccharomycete and rhizobium.Further, Bacillus acidi lactici, saccharomycete and the weight of rhizobium
Amount is than being preferably 2:2:1；The inoculum concentration of the strain is 105-108CFU/ml, i.e., the filtrate inoculation of every the first fermentation liquid of 1ml
105-108The strain of CFU.In some specific embodiments, the inoculum concentration of the strain is 107CFU/ml。
After obtaining the second fermentation liquid, the second fermentation liquid is inactivated, filtering obtains the Whitening, spot composition.Some realities
It applies in scheme, the inactivation is at 60-80 DEG C, holding 15-20min is inactivated by the second fermentation liquid.Some embodiments
In, described be filtered into first carries out primary filtration with 800 mesh mesh screens, is then filtered again with 0.22um filter membrane.
The present invention also provides in a kind of whitening patch-lightening cosmetic, including Whitening, spot composition of the present invention and cosmetics
Acceptable auxiliary material.
Wherein, the whitening patch-lightening cosmetic can be face cream, sun screen, suncream, mildy wash, lotion, toner, face
Film or essence dew.
In some embodiments, based on mass fraction, the whitening patch-lightening cosmetic includes:
1~5% Whitening, spot composition of the present invention, 1%PEG-100 stearate, 1% stearine, 2% spermaceti
Stearyl alcohol, 1.5% cetearyl glucoside, 3% butanediol, 2% docosyl alcohol, 4% glycerol, 0.5% trehalose, 2% are to day
Sunflower seed extract, 0.6~0.8% preservative and excess water.
In some specific embodiments, whitening patch-lightening cosmetic of the present invention is Whitening, spot face cream, based on mass fraction, packet
It includes:
1% Whitening, spot composition of the present invention, 1%PEG-100 stearate, 1% stearine, 2% spermaceti are hard
Lipidol, 1.5% cetearyl glucoside, 3% butanediol, 2% docosyl alcohol, 4% glycerol, 0.5% trehalose, 2% sunflower
Seed extract, 81.4% water, 0.6% preservative.
In some specific embodiments, whitening patch-lightening cosmetic of the present invention is Whitening, spot face cream, based on mass fraction, packet
It includes:
3% Whitening, spot composition of the present invention, 1%PEG-100 stearate, 1% stearine, 2% spermaceti are hard
Lipidol, 1.5% cetearyl glucoside, 3% butanediol, 2% docosyl alcohol, 4% glycerol, 0.5% trehalose, 2% sunflower
Seed extract, 81.2% water, 0.8% preservative.
In some specific embodiments, whitening patch-lightening cosmetic of the present invention is Whitening, spot face cream, based on mass fraction, packet
It includes:
5% Whitening, spot composition of the present invention, 1%PEG-100 stearate, 1% stearine, 2% spermaceti are hard
Lipidol, 1.5% cetearyl glucoside, 3% butanediol, 2% docosyl alcohol, 4% glycerol, 0.5% trehalose, 2% sunflower
Seed extract, 81.4% water, 0.6% preservative.
Whitening, spot composition provided by the invention is formed by the fermenting raw materials of following parts by weight: 5-15 parts of Rhizoma Chuanxiong, red stone
6-10 parts of pomegranate, 2-5 parts of cordate houttuynia, 15-20 parts of kuh-seng, turn round cactus 10-18 parts and purple coneflower 5-8 parts of thorn.This hair of the present invention
The bright plant for piercing six kinds of green naturals of cactus and purple coneflower from Rhizoma Chuanxiong, red pomegranate, cordate houttuynia, kuh-seng, torsion using fermentation technique
The active nutrients that more horn of plenty and more small molecule are extracted in object enhance plant material effect, reduce toxic side effect, peace
Full property greatly improves.Experiment shows that the present composition has tyrosinase and significantly inhibits effect, and cosmetics obtained are to skin
Skin melanin content has stronger inhibiting effect, can be obviously improved skin brightness, has Whitening, spot effect outstanding.
Specific embodiment
The invention discloses Whitening, spot composition and containing the cosmetics of the composition, those skilled in the art can be with
Present disclosure is used for reference, realization of process parameters is suitably modified.In particular, it should be pointed out that all similar substitutions and modifications are to ability
It is it will be apparent that they are considered as being included in the present invention for field technique personnel.Method and application of the invention has been led to
Preferred embodiment is crossed to be described, related personnel obviously can not depart from the content of present invention, in spirit and scope to this paper institute
The methods and applications stated are modified or appropriate changes and combinations, carry out implementation and application the technology of the present invention.
To the explanation of the disclosed embodiments, enable those skilled in the art to implement or use the present invention.To this
A variety of modifications of a little embodiments will be readily apparent to those skilled in the art, as defined herein general
Principle can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore, the present invention will not
It can be intended to be limited to the embodiments shown herein, and be to fit to consistent with the principles and novel features disclosed in this article
Widest scope.
The test material that the present invention uses is all common commercially available product, can all be bought in market.
Below with reference to embodiment, the present invention is further explained:
Embodiment 1
A kind of plant fermentation composition of Whitening, spot provided by the invention is made: Rhizoma Chuanxiong 5 of the component of following weight number
Part, 6 parts of red pomegranate, 2 parts of cordate houttuynia, 15 parts of kuh-seng, 10 parts of cactus of torsion thorn, 5 parts of purple coneflower, preparation method are as follows:
(1) 5 parts of Rhizoma Chuanxiong are weighed, 6 parts of red pomegranate, 2 parts of cordate houttuynia, 15 parts of kuh-seng, turns round 10 parts of cactus of thorn, purple coneflower 5
Part, it is crushed to 50-100 mesh, mixes, obtains mixed-powder；Simultaneously by actication of culture, the strain of selection is Bacillus acidi lactici, yeast
Bacterium, rhizobium, its ratio be 2:2:1, bacterial concentrations 105-108CFU/ml；
(2) above-mentioned 10~15 times of mixed-powder weight of water is added, carries out the first fermentation using 121 DEG C of herb fermenting tank,
Fermentation time is 90min, takes the first filtering fermentation liquor, retains filtrate, then filtrate is carried out high temperature under conditions of 121 DEG C and gone out
Bacterium；
(3) at 28-37 DEG C of temperature, pH value 4-6.5, revolving speed 100-250r/min, and dissolved oxygen amount is 100-150%'s
Under the conditions of the strain activated is inoculated on the filtrate after step (2) high-temperature sterilization, inoculum concentration 107CFU/ml carries out the
Secondary fermentation 48-72h obtains the second fermentation liquid；
(4) the second fermentation liquid is inactivated under the conditions of 60-80 DEG C, 15-20min；Then it is carried out using 800 mesh mesh screens
Primary filtration is finally filtered again with 0.22um filter membrane, obtains tunning.
Embodiment 2
A kind of plant fermentation composition of Whitening, spot provided by the invention is made of the component of following weight number:
8 parts of Rhizoma Chuanxiong, 7 parts of red pomegranate, 3 parts of cordate houttuynia, 16 parts of kuh-seng, 12 parts of cactus of thorn is turned round, 5 parts of purple coneflower, is prepared
Method is the same as embodiment 1；
Embodiment 3
A kind of plant fermentation composition of Whitening, spot provided by the invention is made: Rhizoma Chuanxiong of the component of following weight number
10 parts, 8 parts of red pomegranate, 4 parts of cordate houttuynia, 18 parts of kuh-seng, 15 parts of cactus of torsion thorn, 6 parts of purple coneflower, the same embodiment of preparation method
1；
Embodiment 4
A kind of plant fermentation composition of Whitening, spot provided by the invention is made: Rhizoma Chuanxiong of the component of following weight number
13 parts, 8 parts of red pomegranate, 4 parts of cordate houttuynia, 18 parts of kuh-seng, 16 parts of cactus of torsion thorn, 7 parts of purple coneflower, the same embodiment of preparation method
1。
Embodiment 5
A kind of plant fermentation composition of Whitening, spot provided by the invention is made: Rhizoma Chuanxiong of the component of following weight number
15 parts, 10 parts of red pomegranate, 5 parts of cordate houttuynia, 20 parts of kuh-seng, 18 parts of cactus of thorn is turned round, 8 parts of purple coneflower, preparation method is the same as implementation
Example 1.
Comparative example 1
This comparative example provides a kind of Whitening, spot plant extract composition, is made of the component of following weight number:
5 parts of Rhizoma Chuanxiong extract, 6 parts of red pomegranate extract, 2 parts of houttuynia extract, 15 parts of shrubby sophora extract, turn round thorn celestial being
10 parts of extract of the palm, 5 parts of Echinacea angustifolia extract, extract conventionally extracts, extracting method are as follows: will be each
Plant is cleaned, and dehydrates and crushes, and is that 1:20 adds water to carry out primary filtration, acetic acid after 90 DEG C of water-baths extract 3h by solid-to-liquid ratio
80 DEG C of extraction 2h of ethyl ester, then vacuum concentration is dried to obtain the extract of each plant.
Comparative example 2
The fermentation composition that this comparative example provides, is made of the component of following parts by weight:
5 parts of Rhizoma Chuanxiong, 2 parts of cordate houttuynia, 15 parts of kuh-seng, 10 parts of cactus of thorn is turned round, the preparation method is the same as that of Example 1.
Comparative example 3
The fermentation composition that this comparative example provides, is made of the component of following parts by weight:
5 parts of Rhizoma Chuanxiong, 15 parts of kuh-seng, 10 parts of cactus of thorn is turned round, 5 parts of purple coneflower, the preparation method is the same as that of Example 1.
Comparative example 4
The fermentation composition that this comparative example provides, is made of the component of following parts by weight:
5 parts of Rhizoma Chuanxiong, 6 parts of red pomegranate, 10 parts of cactus of thorn is turned round, 2 parts of cordate houttuynia, the preparation method is the same as that of Example 1.
The measurement of the external tyrosinase inhibition rate of embodiment 6
Experimental method: carrying out the measurement of external tyrosinase inhibition rate using biochemical-Enzymic method, weighs embodiment 1-5 respectively
And comparative example 1-4 composition and be loaded according to table 1, mix, 37 DEG C of water-baths heat preservation, concentration, which is added, in C1 and T1 pipe is
The L-3,4 dihydroxyphenylalanine of 100U/mL mixes.Make reference with C2 after 10min and survey light absorption value of the C1 at 475nm, makees reference survey T1 with T2 and exist
Light absorption value at 475nm.Every group of experiment is 3 times parallel, calculates average value, calculates inhibition of the sample to tyrosinase using formula
Rate.
Tyrosinase inhibition rate=(C1-T1)/C1
The external biochemical enzyme process that table 1 is embodiment 1-5 and comparative example 1-4 detects tyrosinase
Inhibiting rate of the table 2 embodiment 1-5 and comparative example 1-4 to tyrosinase
Experimental result:
The positive reference substance that biochemical-Enzymic method uses is β-arbutin, and the whitening inhibitor is wide as conventional whitening additive
General use is in skin-lightening cosmetic.Experimental data shows the plant fermentation composition of the Whitening, spot of embodiment 1-5 to tyrosinase
Inhibit β-arbutin than comparative example 1-4 and 2% content high to the inhibiting rate of tyrosinase, and mentioned by fermentation technique
The traditional extractive technique of the ratio taken out extracts high to tyrosinase inhibition rate, and it is good to illustrate that the present composition has
Whitening effect, wherein 3 composition of embodiment is optimal combination to the inhibiting rate highest of tyrosinase.
Embodiment 7
A kind of face cream of Whitening, spot, by weight component meter, include following component: whitening made from 1% embodiment 3 is light
Plant fermentation composition, 1%PEG-100 stearate, 1% stearine, 2% cetostearyl alcohol, 1.5% spermaceti of spot
Stearyl glucoside, 3% butanediol, 2% docosyl alcohol, 4% glycerol, 0.5% trehalose, 2% sunflower seed extract, 81.4%
Water, 0.6% preservative.
The preparation method of above-mentioned whitening and spot eliminating cream, comprising the following steps:
(1) by 1%PEG-100 stearate, 1% stearine, 2% cetostearyl alcohol, 1.5% cetearyl
Glucoside is added in oily phase pot, is heated to 75-80 DEG C；
(2) again by 3% butanediol, 2% docosyl alcohol, 4% glycerol, 0.5% trehalose, addition water phase pot, after being uniformly dispersed,
It is heated to 75-80 DEG C；
(3) mixed material of step (1) and step (2) being transferred in emulsion pot, homogeneous 5min is cooled to 45 DEG C,
Whitening, spot composition made from 1% embodiment 3,2% sunflower seed extract, 0.6% preservative are added, continues to be cooled to
It 35 DEG C, stirs evenly to get whitening and spot eliminating cream.
Embodiment 8
A kind of face cream of Whitening, spot, by weight component meter, include following component: whitening made from 3% embodiment 3 is light
Plant fermentation composition, 1%PEG-100 stearate, 1% stearine, 2% cetostearyl alcohol, 1.5% spermaceti of spot
Stearyl glucoside, 3% butanediol, 2% docosyl alcohol, 4% glycerol, 0.5% trehalose, 2% sunflower seed extract, 81.2%
Water, 0.8% preservative.
Preparation method is the same as embodiment 7.
Embodiment 9
A kind of face cream of Whitening, spot, by weight component meter, include following component: whitening made from 5% embodiment 3 is light
Plant fermentation composition, 1%PEG-100 stearate, 1% stearine, 2% cetostearyl alcohol, 1.5% spermaceti of spot
Stearyl glucoside, 3% butanediol, 2% docosyl alcohol, 4% glycerol, 0.5% trehalose, 2% sunflower seed extract, 81.4%
Water, 0.6% preservative.
Preparation method is the same as embodiment 7.
The whitening of 10 whitening spot-relieving cream of embodiment is tested
1. safety evaluatio
1.1 experimental principle
Human experimentation forms test population by particular experiment crowd, and test subject uses cosmetics and cosmetics function
A possibility that causing human skin adverse reaction before and after effect ingredient.
1.2 test material
Spot tester: happy up to Science and Technology Development Co., Ltd., round, diameter 12mm purchased from Beijing 10,000,000,000.
Spot tries object: embodiment 7, embodiment 8, embodiment 9
1.3 Subject Populations and testing time
Effective subject amounts to 30 people, and determination (meets 2007 " cosmetics healths at random for specific sex composition and age composition
Specification " be included in, exclusion criteria).
Testing time: 48h.
1.4 experimental method
Subject is selected by subject enrollment standard, number is 30 people.Qualified patch material is selected, sample is put into spot
It tries in device (amount of samples 0.020g-0.025g), control wells are blank (not setting any substance).Sample and blank control is equal
It is affixed on the forearm song side of subject, is gently pressed with palm and is allowed to equably be pasted on skin, continues to remove patch device afterwards for 24 hours, to
Impression observes dermoreaction after disappearing.And observe primary, observation dermoreaction again respectively with 48h for 24 hours after patch test.
1.5 experimental result
This time patch test results determine the light class's frost of the whitening to people according to the requirement of 2007 " cosmetics health specifications "
Body skin has no adverse reaction.
2. white-skinned face function human trial
2.1 experimental principle
Human experimentation forms test population by particular experiment crowd, and test subject uses cosmetics and cosmetics function
The variation for imitating ingredient front and back dermal melanin and skin brightness, so that it is determined that the white-skinned face function of cosmetics or functional component.
2.2 laboratory apparatus and sample
Instrument: MPA580 Multi-functional skin tester (Mexameter MX18 melanin and ferroheme test probe,
Colorimeter CL400 skin color test probe).
Given the test agent: embodiment 7, embodiment 8, embodiment 9
Blank control group: during test, any cosmetics are not used in recipient site.
2.3 Subject Populations and testing time
Subject amounts to 30 people.Specific sex composition determines at random.Volunteer's exclusion condition meets " cosmetics contact skin
Inflammation diagnostic criteria and treatment principle " it is included in, exclusion criteria.
2.4 experimental method
Select the left and right arm of subject as tested region.MPA580 Multi-functional skin tester is used by technical staff
Smear the dermal melanin and skin brightness before cosmetics in measurement experiment position.It was cleaned sooner or later in process clear water daily tested
Area's (the same area), smear testing product, during experiment, subject Test sites cannot apply erase it is other outside tested sample
Any cosmetics.Subject is after being used continuously cosmetics one week, two weeks, three weeks and four weeks, and same time subject will weekly
It smears position to clean, sit quietly 15min in fixed test environment, smears number positional using test equipment measurement by technical staff
Value.The finally numerical value that statistics subject position measures, the changing rule of analyzing skin melanin and skin brightness L value.
2.5 interpretation of result
2.5.1 melanin content mutation analysis
Dermal melanin changes of contents was reflected in test period, and Experimental Area dermal melanin content changes over time rule
Rule.Its value is bigger, and dermal melanin content is bigger, conversely, dermal melanin content is few.Melanin content difference (Δ T)=make
Melanin content (T0) before product is used with time point melanin content (Tn)-each after product.
The difference of table 3 recipient site dermal melanin content and initial value
2.5.2 skin brightness mutation analysis
Skin brightness variation was reflected in test period, and Experimental Area skin brightness changes with time rule.Its value is got over
Greatly, skin brightness is better, conversely, skin brightness is poorer.Luminance difference (Δ T)=use each time point brightness value after product
(Tn)-use brightness value (T0) before product.
The difference of table 4 recipient site skin brightness content and initial value
2.6 conclusion
9 three sections of embodiment 7, embodiment 8, embodiment whitening spot-relieving creams, which have dermal melanin content, makees strong inhibition
With, while it being able to ascend skin brightness, Comprehensive Correlation, 8 effect of embodiment more protrudes.
3. product testing
The Indexs measures such as sense organ, microorganism, heavy metal, physics and chemistry are carried out to the formula of embodiment 8, and to whitening spot-relieving cream into
Row stability test, (45 ± 1) DEG C keep for 24 hours, restoring to lotion after room temperature without water-oil separating phenomenon, -5 DEG C~-10 DEG C guarantors
It holds for 24 hours, preceding without obvious Traits change with test after restoring to room temperature, test result meets GB/T1857-2004 moisturizing cream state
The requirement of family's standard.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of Whitening, spot composition, which is characterized in that formed by the fermenting raw materials of following parts by weight:
5-15 parts of Rhizoma Chuanxiong, 6-10 parts of red pomegranate, 2-5 parts of cordate houttuynia, 15-20 parts of kuh-seng, turn round cactus 10-18 parts of thorn and purple pine nut
5-8 parts of chrysanthemum.
2. Whitening, spot composition according to claim 1, which is characterized in that each raw material weight number are as follows:
10 parts of Rhizoma Chuanxiong, 8 parts of red pomegranate, 4 parts of cordate houttuynia, 18 parts of kuh-seng, turn round 15 parts and 6 parts of purple coneflower of thorn cactus.
3. Whitening, spot composition according to claim 1, which is characterized in that the strain of the fermentation is lactic acid bar
Bacterium, saccharomycete and rhizobium it is one or more.
4. Whitening, spot composition according to claim 1, which is characterized in that the strain of the fermentation includes lactic acid bar
Bacterium, saccharomycete and rhizobium；The Bacillus acidi lactici, saccharomycete and the weight ratio of rhizobium are 2:2:1.
5. the preparation method of any one of Claims 1 to 4 Whitening, spot composition characterized by comprising
After Rhizoma Chuanxiong, red pomegranate, cordate houttuynia, kuh-seng, torsion thorn cactus and purple coneflower are crushed, mixed, adds water to carry out first and send out
Ferment obtains the first fermentation liquid；
By the first filtering fermentation liquor, filtrate is inoculated with strain after high-temperature sterilization and carries out second of fermentation, obtains the second fermentation liquid；
Second fermentation liquid is inactivated, filtering obtains the Whitening, spot composition.
6. preparation method according to claim 5, which is characterized in that the strain includes Bacillus acidi lactici, saccharomycete and root
Tumor bacterium, the Bacillus acidi lactici, saccharomycete and the weight ratio of rhizobium are 2:2:1；The inoculum concentration of the strain is 105-108CFU/
ml。
7. preparation method according to claim 5, which is characterized in that the temperature of first fermentation is 121 DEG C, when fermentation
Between be 90min.
8. preparation method according to claim 5, which is characterized in that second fermentation is at 28-37 DEG C of temperature, pH value
For 4-6.5, revolving speed 100-250r/min, dissolved oxygen amount carries out under conditions of being 100-150%, fermentation time 48-72h.
9. a kind of whitening patch-lightening cosmetic, including the described in any item Whitening, spot compositions of Claims 1 to 4 or claim
Acceptable auxiliary material in Whitening, spot composition made from any one of 5~8 preparation methods and cosmetics.
10. cosmetics according to claim 9, which is characterized in that based on mass fraction, including 1~5% claim
1~4 described in any item Whitening, spot compositions and following components:
1%PEG-100 stearate, 1% stearine, 2% cetostearyl alcohol, 1.5% cetearyl glucoside,
3% butanediol, 2% docosyl alcohol, 4% glycerol, 0.5% trehalose, 2% sunflower seed extract, 0.6~0.8% preservative and
Excess water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910429141.8A CN110051590A (en) | 2019-05-22 | 2019-05-22 | Whitening, spot composition and cosmetics containing the composition |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910429141.8A CN110051590A (en) | 2019-05-22 | 2019-05-22 | Whitening, spot composition and cosmetics containing the composition |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110051590A true CN110051590A (en) | 2019-07-26 |
Family
ID=67324110
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910429141.8A Pending CN110051590A (en) | 2019-05-22 | 2019-05-22 | Whitening, spot composition and cosmetics containing the composition |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110051590A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113041179A (en) * | 2019-12-27 | 2021-06-29 | 广州市科能化妆品科研有限公司 | Moisturizing cream, preparation method thereof and collagenase inhibitor |
| CN113730320A (en) * | 2021-09-27 | 2021-12-03 | 广州睿晞生化科技有限公司 | Moisturizing composition, application thereof and cosmetic |
| WO2023126396A1 (en) * | 2021-12-31 | 2023-07-06 | L'oreal | Customizable texture cosmetic composition |
| CN117646038A (en) * | 2024-01-30 | 2024-03-05 | 妙越缇卡生物医药科技(山东)有限公司 | Lactobacillus paracasei SF-L31 fermented product for whitening and lightening spots and application thereof |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150098934A1 (en) * | 2013-10-03 | 2015-04-09 | Wilson A. Lee | Topical Preparations Comprising Grape Seed, Folic Acid, Biotin, Bifidobacterium longum and Echinacea purpurea |
| CN105147584A (en) * | 2015-09-24 | 2015-12-16 | 广州环亚化妆品科技有限公司 | Traditional-Chinese-medicine-ingredient-containing yeast fermentation material, preparing method thereof and application thereof |
| CN106176566A (en) * | 2016-08-23 | 2016-12-07 | 上海新高姿化妆品有限公司 | Cosmetic composition with white-skinned face function and preparation method thereof |
| JP2017052706A (en) * | 2015-09-07 | 2017-03-16 | 一丸ファルコス株式会社 | Cosmetic composition containing granules |
| KR20170095672A (en) * | 2016-02-13 | 2017-08-23 | 류기찬 | Cleaning composition for containing the extract using mixed microorganisms. |
| CN108042433A (en) * | 2018-01-17 | 2018-05-18 | 广州环亚化妆品科技有限公司 | A kind of whitening antioxidation plant composition, plant composition zymotic fluid and preparation method and purposes |
| CN108619076A (en) * | 2018-06-21 | 2018-10-09 | 佛山汇沐化学科技有限公司 | A kind of skin whitening, moisturizing lotion being suitable for sensitive skin |
| CN108670943A (en) * | 2018-09-06 | 2018-10-19 | 浙江施维康科技有限公司 | A kind of moisturizing, whitening, light spot composition of plant extracts |
| CN109276529A (en) * | 2018-09-28 | 2019-01-29 | 康美时代（广东）发展有限公司 | A kind of lightening compositions and the whitening lotion comprising the composition |
| CN109363998A (en) * | 2018-12-26 | 2019-02-22 | 广州市科能化妆品科研有限公司 | Skin-lightening cosmetic additive and whitening essence stoste and preparation method thereof |
-
2019
- 2019-05-22 CN CN201910429141.8A patent/CN110051590A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150098934A1 (en) * | 2013-10-03 | 2015-04-09 | Wilson A. Lee | Topical Preparations Comprising Grape Seed, Folic Acid, Biotin, Bifidobacterium longum and Echinacea purpurea |
| JP2017052706A (en) * | 2015-09-07 | 2017-03-16 | 一丸ファルコス株式会社 | Cosmetic composition containing granules |
| CN105147584A (en) * | 2015-09-24 | 2015-12-16 | 广州环亚化妆品科技有限公司 | Traditional-Chinese-medicine-ingredient-containing yeast fermentation material, preparing method thereof and application thereof |
| KR20170095672A (en) * | 2016-02-13 | 2017-08-23 | 류기찬 | Cleaning composition for containing the extract using mixed microorganisms. |
| CN106176566A (en) * | 2016-08-23 | 2016-12-07 | 上海新高姿化妆品有限公司 | Cosmetic composition with white-skinned face function and preparation method thereof |
| CN108042433A (en) * | 2018-01-17 | 2018-05-18 | 广州环亚化妆品科技有限公司 | A kind of whitening antioxidation plant composition, plant composition zymotic fluid and preparation method and purposes |
| CN108619076A (en) * | 2018-06-21 | 2018-10-09 | 佛山汇沐化学科技有限公司 | A kind of skin whitening, moisturizing lotion being suitable for sensitive skin |
| CN108670943A (en) * | 2018-09-06 | 2018-10-19 | 浙江施维康科技有限公司 | A kind of moisturizing, whitening, light spot composition of plant extracts |
| CN109276529A (en) * | 2018-09-28 | 2019-01-29 | 康美时代（广东）发展有限公司 | A kind of lightening compositions and the whitening lotion comprising the composition |
| CN109363998A (en) * | 2018-12-26 | 2019-02-22 | 广州市科能化妆品科研有限公司 | Skin-lightening cosmetic additive and whitening essence stoste and preparation method thereof |
Non-Patent Citations (5)
| Title |
|---|
| 侯在恩: "《美容实用化妆学》", 30 June 2009 * |
| 夏逸: "《必知的30种HOT美肌营养素》", 28 February 2007 * |
| 张虹 等: "《一本书读懂百花治百病》", 31 October 2014 * |
| 江玲: "紫花松果菊菊苣酸提取与生物活性研究", 《中国优秀硕士学位论文全文数据库 (工程科技Ⅰ辑)》 * |
| 黄丽萍 等: "《美容中药方剂学》", 28 February 2013 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113041179A (en) * | 2019-12-27 | 2021-06-29 | 广州市科能化妆品科研有限公司 | Moisturizing cream, preparation method thereof and collagenase inhibitor |
| CN113041179B (en) * | 2019-12-27 | 2023-10-27 | 广州市科能化妆品科研有限公司 | Moisturizing cream and preparation method thereof and collagenase inhibitor |
| CN113730320A (en) * | 2021-09-27 | 2021-12-03 | 广州睿晞生化科技有限公司 | Moisturizing composition, application thereof and cosmetic |
| CN113730320B (en) * | 2021-09-27 | 2023-04-07 | 广州睿晞生化科技有限公司 | Moisturizing composition, application thereof and cosmetic |
| WO2023126396A1 (en) * | 2021-12-31 | 2023-07-06 | L'oreal | Customizable texture cosmetic composition |
| FR3131531A1 (en) * | 2021-12-31 | 2023-07-07 | L'oreal | Cosmetic composition with customizable texture |
| CN117646038A (en) * | 2024-01-30 | 2024-03-05 | 妙越缇卡生物医药科技(山东)有限公司 | Lactobacillus paracasei SF-L31 fermented product for whitening and lightening spots and application thereof |
| CN117646038B (en) * | 2024-01-30 | 2024-04-19 | 妙越缇卡生物医药科技(山东)有限公司 | Lactobacillus paracasei SF-L31 fermented product for whitening and lightening spots and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110051590A (en) | Whitening, spot composition and cosmetics containing the composition | |
| CN106727147B (en) | Draft American ginseng beautifying skin improves drug and cosmetic applications and preparation | |
| KR100982883B1 (en) | Cosmetic composition comprising mixture extract and preparation thereof | |
| CN109528603A (en) | Lightening compositions, whitening brightening cream comprising lightening compositions and preparation method thereof | |
| CN108042433B (en) | Whitening antioxidant plant composition, plant composition fermentation liquor, preparation method and application | |
| CN108014045B (en) | Man skin care composition and application thereof | |
| CN110251432A (en) | A kind of acne-removing composition containing centella and application | |
| CN107550775A (en) | Natural plants anti-acne jelly and preparation method thereof | |
| EP3552599A1 (en) | Composition containing crocus sativus extract and preparation method and use thereof | |
| CN108096146A (en) | With whitening and anti-aging, the rose enzyme of anti-inflammatory effect and its application in cosmetics | |
| CN105560157A (en) | Novel application of Cordyceps fungus extract | |
| CN115554198B (en) | Anti-inflammatory and soothing composition and application thereof | |
| CN107184482A (en) | Mateine and its application in skin preparations for extenal use | |
| CN105925617A (en) | Radix pueraiae fermentation protoplasm cosmetic product and preparation method and application thereof | |
| CN105998617A (en) | Externally applied traditional Chinese medicine extract with effect of soothing senile pruritus, as well as preparation method and application thereof | |
| CN109394640A (en) | A kind of ferment skin care compositions and its application | |
| CN107802545A (en) | A kind of anti-oxidant and lightening compositions and the skin care item containing said composition | |
| CN106580833A (en) | Whitening moisturizing cream | |
| KR20180020574A (en) | Cosmetic compositon for removing heavy metal or fine dust containing extracts of rhapis excelsa and ficus robusta | |
| CN114767608B (en) | Natural plant maintenance refreshing Yan Fupei skin care product raw material, preparation method and application thereof | |
| CN109288754A (en) | A kind of Essence and preparation method containing persimmon leaf extractive from fermentative | |
| CN109620782A (en) | A kind of plant composition with Whitening, spot effect | |
| CN109276519A (en) | A kind of paris polyphylla fermenation raw liquid and its preparation method and application | |
| CN113876636A (en) | Microemulsion gel mask | |
| KR101583052B1 (en) | Anti-wrinkle cosmetic composition containing Polygonum multiflorum extract |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190726 |
|
| RJ01 | Rejection of invention patent application after publication |