CN118546197B - Separation and purification process of calpain - Google Patents
Separation and purification process of calpain Download PDFInfo
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- CN118546197B CN118546197B CN202411020610.8A CN202411020610A CN118546197B CN 118546197 B CN118546197 B CN 118546197B CN 202411020610 A CN202411020610 A CN 202411020610A CN 118546197 B CN118546197 B CN 118546197B
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- 102000007590 Calpain Human genes 0.000 title claims abstract description 22
- 108010032088 Calpain Proteins 0.000 title claims abstract description 22
- 238000000746 purification Methods 0.000 title claims abstract description 12
- 238000000926 separation method Methods 0.000 title claims abstract description 12
- 239000002994 raw material Substances 0.000 claims abstract description 85
- 238000004140 cleaning Methods 0.000 claims abstract description 22
- 238000009826 distribution Methods 0.000 claims abstract description 14
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 14
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 14
- 239000011232 storage material Substances 0.000 claims abstract description 4
- 238000010438 heat treatment Methods 0.000 claims description 129
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- 238000010411 cooking Methods 0.000 claims description 19
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- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 abstract description 8
- 229910052791 calcium Inorganic materials 0.000 abstract description 8
- 239000011575 calcium Substances 0.000 abstract description 8
- 238000004519 manufacturing process Methods 0.000 abstract description 2
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
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Abstract
The application discloses a separation and purification process of calpain, which relates to the technical field of production and processing of calpain products, and comprises the following steps: stock call, comprising: evaluating the aggregate demand of the birds and the shell raw material demand based on the demand information and a preset batching rule, calling storage materials and implementing the next step on the shell raw material; a cleaning pretreatment comprising: receiving shell raw materials provided by suppliers and recording corresponding growth information of aquatic products and shelling information of the suppliers; searching a preset experience database according to the growth information and the shelling information, and evaluating the residual ratio; the shell raw materials are cured in turn in a low-temperature and high-temperature treatment mode for a plurality of times, and the high-temperature and low-temperature treatment time distribution and the temperature distribution are adjusted according to the residual proportion; cleaning raw materials; and (5) separating and purifying. The application has the advantages of better treatment of the raw materials for preparing the calcium protein powder and improvement of the uniformity of the product quality.
Description
Technical Field
The application relates to the technical field of production and processing of calpain products, in particular to a separation and purification process of calpain.
Background
The calpain oral liquid, the protein powder and the like can be used for treating health problems such as osteoporosis and the like of human bodies.
The preparation of the above products requires the use of calpain, which can be extracted from the bones of the birds. Considering the aquatic products such as shrimps and crabs, the delicious taste is popular with masses, and the demand market is increasingly growing, so that various aquatic product processing centers can generate a large amount of shrimp shells and the like every year except families, and a large amount of biomass garbage can be generated if the shrimp shells are discarded. The related data such as paper (research on bioavailability of composite calcium powder of protein hydrolysate-calcium citrate prepared from shrimp shells) indicates that biomass such as shrimp shells can be reused as raw materials for preparing calcium-supplementing products, so I consider that the biomass is used together with bones of livestock and poultry, and limit of the preparation of related products by using the raw materials of calpain is reduced.
At present, there are at least two ways to prepare calpain by using shrimp shells and the like: 1. after the shrimp shell is cleaned and dried, grinding the shrimp shell into powder, and mixing the powder with other edible powder for use; 2. soaking with reagent (such as:) and separating and purifying the leaching solution to prepare calcium protein powder. Compared with the former, the latter treatment mode has higher product purity, but residual meat and the like in shrimp shells are difficult to clean, the raw material treatment difficulty is higher, and the quality of each batch of produced protein powder is relatively easy to be inconsistent, so the application provides a new technical scheme.
Disclosure of Invention
In order to better process the preparation raw materials of the calcium protein powder and improve the uniformity of the quality of the product, the application provides a separation and purification process of the calcium protein.
The application provides a separation and purification process of calpain, which adopts the following technical scheme:
a process for separating and purifying calpain, comprising:
step one, reserve material calling, which comprises the following steps:
receiving the requirement information of protein preparation initiated by staff;
Evaluating the aggregate demand of the birds and the shell raw material demand based on the demand information and a preset batching rule, calling storage materials, and implementing the next step on the shell raw material;
step two, cleaning pretreatment, which comprises the following steps:
s11, receiving shell raw materials provided by suppliers, and recording corresponding aquatic product growth information and shell removing information of the suppliers; wherein the growth information comprises growth time, size and molting record;
s12, searching a preset experience database according to the growth information and the shelling information, and evaluating the residual ratio;
S13, curing the shell raw materials in a low-temperature and high-temperature treatment mode for a plurality of times in turn, and adjusting the time distribution and the temperature distribution of the high-temperature and low-temperature treatment according to the residual occupation ratio;
step three, raw material cleaning, which comprises the following steps: cleaning and drying the cured shell raw materials;
step four, separation and purification, which comprises:
soaking the raw materials prepared in the third step in a preselected precipitation solution, and filtering after soaking to obtain a leaching solution;
regulating the PH value of the leaching solution, and purifying and separating out calpain;
And (5) centrifuging to obtain a stock solution.
Optionally, the adjusting the high-low temperature processing time distribution and the temperature distribution according to the residual duty ratio includes:
S21, defining a freezing and curing temperature interval and a high-temperature curing temperature interval, defining a data relationship between a residual rate of temperature rise and a high-temperature curing temperature, and recording the data relationship as an empirical data table;
S22, cooling the raw materials by using refrigeration equipment until the temperature reaches a freezing temperature interval, and maintaining the time t 1; wherein the time t1 is a preset value;
S23, heating the frozen raw materials at d1 rate through heating equipment, and heating to normal temperature; feeding raw materials at normal temperature into a cooking chamber for heating, heating to the temperature T1, and maintaining the time T2; the d1 rate and the T1 temperature are determined by searching an empirical data table, the T1 temperature is in a high-temperature curing temperature interval, and the T2 duration is a preset value;
s24, fishing out the raw materials;
s25, repeating the steps S21-S24 for n1 times, and preparing for cleaning; wherein n1 is a preset value.
Optionally, the heating device includes:
A microwave heating structure for heating the raw material to normal temperature;
A cooking structure for heating a raw material at normal temperature to a T1 temperature;
a temperature detection component for detecting the temperature of the raw materials in the microwave heating structure and the cooking structure;
A controller connected to the microwave heating structure, the cooking structure and the temperature detecting assembly;
The microwave heating structure is provided with a first heating cavity, and a movable electric partition plate is arranged at the bottom opening of the first heating cavity;
the cooking structure is internally provided with a heating cavity II, the upper part of the heating cavity II is opened and is separated from the heating cavity I through an electric partition board, the heating cavity II is communicated with an outward pressure regulating pipe, the outward end of the pressure regulating pipe is provided with an electric three-way valve, one of the other two ports of the electric three-way valve is communicated to the heating cavity I through a pipeline, and the other port of the electric three-way valve is communicated with a heat exchange facility;
The electric three-way valve and the electric partition are electrically connected to a controller configured to: and controlling the microwave heating structure according to the d1 rate to heat.
Optionally, the controller is configured to:
if the temperature of the raw materials in the first heating cavity is normal temperature, controlling the electric partition plate to be opened;
And if the temperature in the heating cavity II is higher than the temperature T1 and the temperature of the raw materials in the heating cavity I is lower than the normal temperature, controlling the electric three-way valve to be communicated with the heating cavity I.
Optionally, a steam pipeline penetrates through the cavity wall of the first heating cavity, and the outer end of the steam pipeline is communicated with a pipeline at the outer end of the electric three-way valve;
The electric partition plate comprises a plurality of movable plates, a rotating shaft and a first motor, wherein the movable plates are spliced to separate a first heating cavity from a second heating cavity, the rotating shaft is fixed at one end, close to the cavity wall, of the movable plates, the rotating shaft is rotationally connected with the cavity wall of the first heating cavity, and the first motor is used for driving the rotating shaft to rotate and is electrically connected with the controller;
The movable plate is internally provided with a steam channel, at least one steam channel is provided with an air inlet which is in butt joint with a port in the steam pipeline pair when the movable plate is closed, the movable plate is also provided with a plurality of steam holes, and the steam holes are upwards opened and downwards communicated with the steam channel.
Optionally, the heating device further comprises a stirring mechanism, wherein a stirring part of the stirring mechanism stretches into the first heating cavity and is used for stirring the raw materials on the movable plate, and the stirring mechanism is electrically connected to the controller.
Optionally, the rabbling mechanism includes puddler and motor two, the puddler rotates the chamber wall of being connected in heating chamber one, the inside cavity of puddler, temperature detection subassembly is used for detecting the temperature and is regarded as the raw materials temperature in the heating chamber one to the puddler inside.
Optionally, the controller is electrically connected with a pressure detection unit, a probe of the pressure detection unit stretches into the heating cavity II, and the controller is configured to:
And if the heating cavity II accords with a preset opening condition or the heating cavity II accords with a preset pressure relief condition, controlling the electric three-way valve to relieve pressure according to the pressure value fed back by the pressure detection unit.
In summary, the application has the following beneficial technical effects: the process can reduce the difficulty of removing the residual meat in the shell, improve the difficulty of cleaning the shell raw materials, ensure the cleaning effect of the raw materials, and improve the uniformity of the quality of the processed product.
Drawings
FIG. 1 is a schematic flow diagram of the present invention;
FIG. 2 is a second flow chart of the present invention;
FIG. 3 is a schematic view of a control structure of the heating apparatus of the present invention;
fig. 4 is a schematic structural view of the heating apparatus of the present invention.
Reference numerals illustrate: 1. a microwave heating structure; 11. a first heating cavity; 12. a steam pipe; 2. a cooking structure; 21. a heating cavity II; 22. a pressure regulating tube; 23. an electric three-way valve; 3. a temperature detection assembly; 4. a controller; 5. an electric separator; 51. a movable plate; 52. a first motor; 6. a stirring mechanism; 61. a stirring rod; 62. a second motor; 7. and a pressure detection unit.
Detailed Description
The application is described in further detail below with reference to fig. 1-4.
The embodiment of the application discloses a separation and purification process of calpain.
Referring to fig. 1-2, the separation and purification process of calpain includes:
step one, reserve material calling, which comprises the following steps:
Receiving the requirement information of protein preparation initiated by staff; the information required may be how many kg of protein stock is needed.
And evaluating the aggregate demand of the birds and the shell raw material demand based on the demand information and the preset batching rules, calling the storage materials (from a preset warehouse), and implementing the next step on the shell raw material.
Wherein, batching rule examples: requiring 5kg of stock solution, and producing 3.8kg of stock solution by 1000kg of fowl and animal aggregate; 500kg produces 3kg stock solution, the stock solution prepared from aggregate is 80% and 20% of the stock solution is prepared from shells, then 5 x 0.8=4kg stock solution needs to be prepared from fowl and animal aggregate, and 1kg stock solution is prepared from shells; further calculations resulted in the need for 166.7kg of shell material. In this embodiment, the mixing is to consider that the effect of the product is improved by using trace elements in the shell.
Wherein, the aggregate of the livestock and the poultry is firstly cleaned after being selected, then meat, fat and the like remained on the bones are removed manually or by a machine, then the mixture is steamed at high temperature, crushed and soaked, the leaching solution is taken for filtering, the PH value is regulated, and the stock solution is produced by centrifugation. The application focuses on how to treat the shell raw materials, so that the aggregate is not repeated.
Step two, cleaning pretreatment, which comprises the following steps:
s11, receiving shell raw materials provided by suppliers, and recording corresponding aquatic product growth information and shell removing information of the suppliers.
Wherein, the shell material can be shrimp shell in the application; the growth information includes growth time, molting record. The information is obtained because the shrimp shells with different growth time and shell replacement time have different softness and hardness, and the shrimp meat is different in stripping difficulty, so that the residual shrimp meat amount in the shrimp shells is different; according to an example: the present situation of shrimp shelling processing and convenient shelling technology for pretreatment are published in the southern aquatic science.
The provider's shelling information should include at least: a shelling mode, a shelling equipment type; the residual shrimp meat amount in the shrimp shells is also different due to different shelling modes and the like; in this embodiment, the shelling mode may be an extrusion shelling mode exemplified in the above-mentioned article.
S12, searching a preset experience database according to the growth information and the shelling information, and evaluating the residual ratio.
In this embodiment, the method requires the staff to build an experience database in advance, and examples are: taking shrimps under any growth information, dividing the shrimps into a plurality of groups, wherein the number of each group is the same, removing shells in a shell removing mode, and weighing the shrimps in the shells respectively; and taking out the residual meat from the shrimp shells respectively, weighing, calculating the ratio of the residual meat to the weight of the shrimp shells respectively, calculating the average value of the ratio of a plurality of groups, and recording and storing in an experience database.
S13, curing the shell raw materials in a low-temperature and high-temperature treatment mode for a plurality of times in turn, and adjusting the time distribution and the temperature distribution of the high-temperature and low-temperature treatment according to the residual ratio.
It will be appreciated that high temperature cooking can cook food to maturity, freezing aquatic food can destroy internal structure, and if frozen food is warmed up rapidly, it will result in the food being powdered and flocculated. The application utilizes the phenomenon to reduce the adherence of the residual meat in the shrimp shell so as to reduce the difficulty of cleaning the residual meat in the follow-up process.
Step three, raw material cleaning, which comprises the following steps: cleaning and drying the cured shell raw materials.
Wherein, cleaning refers to cleaning the residual shrimp meat in the shrimp shell; drying refers to air-drying and airing the washed shrimp shells so as to store the shrimp shells when the shrimp shells are not used and prevent mildew from mixing with harmful substances such as mould.
The way to clean the shrimp shells may be by a centrifugal machine wash, examples: placing a liner with holes in the centrifugal machine, and more preferably, arranging a stirring rod structure in the liner, and stirring while centrifuging; reference is made to the structural principle of the washing machine.
Step four, separation and purification, which comprises:
Soaking the raw materials prepared in the third step in a preselected precipitation solution (such as ethanol mixed solution with the concentration of 95 percent), and filtering after soaking to obtain a leaching solution; the soaking time can be 18 hours, 24 hours or 36 hours, and the leaching time can be selected according to the protein content measured by taking the leaching solution.
The pH value of the leaching solution is regulated to purify and separate out calpain, the link can be considered to comprise two parts, wherein the first part is used for replacing calcium by hydrochloric acid/carbonic acid regulation reaction; and adjusting the pH value to the protein precipitation isoelectric point to obtain the calpain.
And (3) centrifuging to obtain a stock solution, namely separating out calcium-containing protein stock solution and other waste liquid by centrifuging through a centrifuge, wherein the stock solution can be used together with the calcium-containing protein stock solution prepared from bones of birds and beasts for preparing products such as oral liquid, compound protein powder and the like.
According to the above, the process can reduce the difficulty of removing the residual meat in the shell, improve the difficulty of cleaning the shell raw materials, ensure the cleaning effect of the raw materials, and improve the uniformity of the quality of the processed products.
In another embodiment of the present application, the adjusting the high and low temperature processing time distribution and the temperature distribution according to the residual duty ratio includes:
S21, defining a freezing and curing temperature interval and a high-temperature curing temperature interval, defining a data relationship between a residual rate of temperature rise and a high-temperature curing temperature, and recording the data relationship as an empirical data table;
S22, cooling the raw materials by using refrigeration equipment (such as a refrigeration house and liquid nitrogen heat exchange equipment) until the temperature reaches a freezing temperature interval, and maintaining the time period t 1; wherein, the time t1 is a preset value, and has a proportional relation with the weight of the raw materials, and the larger the weight is, the longer the time t1 is, so as to ensure that the raw materials all enter the freezing temperature;
S23, heating the frozen raw materials at a d1 rate by heating equipment, and heating to normal temperature (such as 22 ℃);
Feeding raw materials at normal temperature into a cooking chamber for heating, heating to the temperature T1, and maintaining the time T2;
Wherein, d1 rate and T1 temperature are determined by looking up an empirical data table and are in a direct proportion relation with the residual ratio, and the larger the residual ratio is, the larger the d1 rate and T1 temperature are; the temperature T1 is in a high-temperature curing temperature range;
s24, fishing out the raw materials;
s25, repeating the steps S21-S24 for n1 times, and preparing for cleaning; wherein n1 is a preset value.
According to the arrangement, the residual meat can be destroyed by using a high-low temperature and rapid temperature rising mode, so that the residual meat is rotten, the shrimp shell is cleaned more simply, and the cleaning effect is better.
Referring to fig. 3 and 4, in another embodiment of the present application, in order to better complete the second step, a heating apparatus is provided including:
A microwave heating structure 1 for heating the raw material to normal temperature;
a steaming structure 2 for heating a raw material at normal temperature to a temperature T1;
A temperature detection unit 3 for detecting the temperature of the raw materials in the microwave heating structure 1 and the cooking structure 2;
a controller 4 connected to the microwave heating structure 1, the cooking structure 2 and the temperature detecting assembly 3.
Wherein, the microwave heating structure 1 is in a hollow square body shape, the heating principle and the structure refer to a microwave oven, at least one heating cavity I11 is arranged, and a microwave generator and a wave guide tube are arranged in the structure to generate microwaves for heating and send the microwaves into the heating cavity I11 through the wave guide tube; the structure of the microwave oven is different from that of a microwave oven: the bottom of the first heating cavity 11 is designed as an opening, and a movable electric baffle 5 is arranged at the opening.
It will be appreciated that the front or side of the heating chamber one 11 should also be open and hinged to the door of the box, this opening being for the staff to feed shrimp shells into the heating chamber one 11.
The cooking structure 2 is also in a hollow square structure, is positioned below the microwave heating structure 1 and is provided with a heating cavity II 21 communicated with the heating cavity I11 after the electric partition board 5 is opened. An electric heating rod is arranged at the bottom or at the side part of the cooking structure 2, a heating cavity II 21 is surrounded on the inner side and is a heat conduction metal inner container, and the outer layer is coated by a heat insulation material; the front or side of the cooking structure 2 can also be designed to be open and hinged to cover the other cover, so that after cooking, the liner is pulled out to fish out the shrimp shell.
The microwave generator and the electric heating rod are electrically connected to the controller 4, and the controller 4 can be an MCU controller, an integrated electronic switch, a voltage-variable circuit, a touch panel and the like for electric control.
The second heating cavity 21 is communicated with an external pressure regulating pipe 22, an electric three-way valve 23 is arranged at the outer end of the pressure regulating pipe 22, one of the other two ports of the electric three-way valve 23 is communicated with the first heating cavity 11 through a pipeline, and the other port is communicated with a heat exchange facility such as a heat exchanger.
The electric three-way valve 23 and the electric separator 5 are electrically connected to the controller 4, and the controller 4 is configured to: controlling the microwave heating structure to heat according to the d1 rate; examples: the microwave heating is divided into three gears of low activity, medium fire and high fire, and each gear corresponds to one d1 speed.
According to the arrangement, when the raw materials are required to be cured, the heat exchange tube of the heat exchanger can be paved on a certain metal and ceramic platform; pouring the frozen raw materials into the platform, placing the first heating cavity 11 in the platform, and heating the platform to normal temperature in a microwave heating mode; then, the electric partition board 5 is opened to send the raw material at normal temperature into the second heating cavity 21, and the temperature is further raised to the temperature T1 for high-temperature curing; in the process, the number of raw material transferring times is reduced, and hot steam generated by the heating cavity II 21 can be reused to reduce heat loss.
Further, the controller 4 is configured to:
if the temperature of the raw materials in the first heating cavity 11 is normal temperature, the electric partition board 5 is controlled to be opened;
If the temperature in the heating cavity two 21 is higher than the temperature T1 and the temperature of the raw material in the heating cavity one 11 is lower than the normal temperature, the electric three-way valve 23 is controlled to be communicated with the heating cavity one 11.
According to the setting, the intelligent degree of the heating equipment is relatively high, raw material and waste heat in the equipment do not need to be manually controlled, and staff does not need to watch at any time, so that the heating equipment is more convenient to use.
In another embodiment of the application, the wall of the first heating cavity 11 is provided with a steam pipeline 12 in a penetrating way, and the outer end of the steam pipeline 12 is communicated with a pipeline opposite to the outer end of the electric three-way valve 23.
The electric partition board 5 includes a plurality of movable boards 51, a rotating shaft and a first motor 52, wherein the number of the movable boards 51 is two for example, and the two movable boards are distributed left and right and are spliced to separate a first heating cavity 11 and a second heating cavity 21. The movable plate 51 is close to one end side face of the cavity wall to fix a rotating shaft, the rotating shaft is rotationally connected with the cavity wall of the heating cavity I11, at least one end of the rotating shaft is transmitted out, the motor I52 is arranged outside the microwave heating structure 1, and an output shaft of the motor I52 is provided with a transmission gear meshed with a gear at the end part of the rotating shaft so as to drive the rotating shaft to rotate; the first motor 52 is electrically connected to the controller 4.
A steam channel is arranged in the movable plate 51, and at least one steam channel is provided with an air inlet which is in butt joint with a port in the steam pipeline 12 pair when the movable plate 51 is closed, namely, horizontally arranged, namely, communication is realized.
The upper part of the movable plate 51 is also provided with a plurality of steam holes which are opened upwards and communicated with the steam channel downwards.
According to the above arrangement, the movable plate 51 is turned downward to feed the raw material into the second heating chamber 21; it should be noted that the side edge of the movable plate 51 may be fixed with a rubber ring to achieve the closing effect of the electric partition 5; in addition, if necessary, an electromagnet may be embedded in the side where the two movable plates 51 are close to each other, so as to enhance the stability during closing. The movable mode of the movable plate 51 is matched with the arrangement of the steam pipeline 12 and the arrangement of the steam running structure on the movable plate 51, so that steam can enter from the lower part of the raw material, and the heat utilization based on the steam is realized. It should be noted that the upper end of each steam hole is provided with a ventilation membrane or cloth so as to prevent the raw materials from blocking the steam hole.
Referring to fig. 4, the heating apparatus further includes a stirring mechanism 6, wherein a stirring portion of the stirring mechanism 6 extends into the first heating chamber 11 and is used for stirring the raw material on the movable plate 51, and the stirring mechanism 6 is electrically connected to the controller.
The stirring mechanism is arranged, so that the microwave heating structure 1 is not a microwave oven after all, particularly, the bottom opening is designed, so that a turntable cannot be designed, and the placed raw materials cannot lean against the turntable to rotate, so that the raw materials are unevenly heated by microwaves; therefore, the application also provides a stirring mechanism 6 for stirring the raw materials; the arrangement is matched with a steam air outlet mode, so that the temperature of the raw materials is relatively even, and the curing effect of the raw materials is relatively balanced.
In one embodiment of the application, the stirring mechanism 6 comprises a stirring rod 61 and a motor II 62, the stirring rod 61 is rotatably connected to the cavity wall of the heating cavity I11, the rod and the blades forming the stirring rod 61 are hollow, the rod part of the stirring rod penetrates out of the rear cavity to be open, and the end part of the stirring rod is fixed with a bevel gear; the output shaft of motor two 62 is fitted with another drive bevel gear that engages the bevel gear described above. The temperature detection assembly 3 comprises a temperature sensor which is fixed on the outer wall of the microwave heating structure 1 through a bracket, and the probe penetrates into the inner cavity of the stirring rod 61 downwards; the other temperature sensor is built in the liquid surface of the heating cavity II 21.
According to the arrangement, the stirring mechanism 6 is utilized to stir the raw materials, and meanwhile, the stirring rod 61 can be utilized to complete the detection of the temperature of the raw materials, so that the temperature detection is more accurate and meets the use requirement of the application compared with the situation that the temperature sensor is directly arranged at the position of the cavity wall and is relatively less disturbed.
Referring to fig. 3, the controller 4 is further electrically connected to a pressure detection unit 7, which may be a gas pressure sensor, with its probe extending into the heating chamber two 21. The controller 4 is configured to: if the heating cavity II 21 meets the preset opening condition or the heating cavity II 21 meets the preset pressure relief condition, the electric three-way valve 23 is controlled to relieve pressure according to the pressure value fed back by the pressure detection unit 7.
The above-mentioned opening conditions are as follows: the preset door opening key on the box cover of the heating cavity II 21 is triggered or heated to the temperature T1, and the time period T2 is maintained. The pressure relief conditions are as follows: the real-time pressure is greater than a preset pressure threshold.
According to the above, on one hand, the probability of steam scalding workers can be reduced; on the other hand, the risk of excessive pressure inside the device can be prevented.
The above embodiments are not intended to limit the scope of the present application, so: all equivalent changes in structure, shape and principle of the application should be covered in the scope of protection of the application.
Claims (4)
1. A process for separating and purifying calpain, which is characterized by comprising the following steps:
step one, reserve material calling, which comprises the following steps:
receiving the requirement information of protein preparation initiated by staff;
Evaluating the aggregate demand of the birds and the shell raw material demand based on the demand information and a preset batching rule, calling storage materials, and implementing the next step on the shell raw material;
step two, cleaning pretreatment, which comprises the following steps:
s11, receiving shell raw materials provided by suppliers, and recording corresponding aquatic product growth information and shell removing information of the suppliers; wherein the growth information comprises growth time, size and molting record;
s12, searching a preset experience database according to the growth information and the shelling information, and evaluating the residual ratio;
S13, curing the shell raw materials in a low-temperature and high-temperature treatment mode for a plurality of times in turn, and adjusting the time distribution and the temperature distribution of the high-temperature and low-temperature treatment according to the residual occupation ratio;
step three, raw material cleaning, which comprises the following steps: cleaning and drying the cured shell raw materials;
step four, separation and purification, which comprises:
soaking the raw materials prepared in the third step in a preselected precipitation solution, and filtering after soaking to obtain a leaching solution;
regulating the PH value of the leaching solution, and purifying and separating out calpain;
centrifuging to obtain stock solution;
the method for adjusting the high-low temperature treatment time distribution and the temperature distribution according to the residual duty ratio comprises the following steps:
S21, defining a freezing and curing temperature interval and a high-temperature curing temperature interval, defining a data relationship between a residual rate of temperature rise and a high-temperature curing temperature, and recording the data relationship as an empirical data table;
S22, cooling the raw materials by using refrigeration equipment until the temperature reaches a freezing temperature interval, and maintaining the time t 1; wherein the time t1 is a preset value;
S23, heating the frozen raw materials at d1 rate through heating equipment, and heating to normal temperature; feeding raw materials at normal temperature into a cooking chamber for heating, heating to the temperature T1, and maintaining the time T2; the d1 rate and the T1 temperature are determined by searching an empirical data table, the T1 temperature is in a high-temperature curing temperature interval, and the T2 duration is a preset value;
s24, fishing out the raw materials;
S25, repeating the steps S21-S24 for n1 times, and preparing for cleaning; wherein n1 is a preset value;
The heating apparatus includes:
A microwave heating structure (1) for heating the raw material to normal temperature;
A cooking structure (2) for heating a raw material at normal temperature to a T1 temperature;
a temperature detection component (3) for detecting the temperature of the raw materials in the microwave heating structure (1) and the cooking structure (2);
A controller (4) connected to the microwave heating structure (1), the cooking structure (2) and the temperature detecting assembly (3);
the microwave heating structure (1) is provided with a first heating cavity (11), and the bottom of the first heating cavity (11) is provided with an opening and a movable electric partition board (5);
the cooking structure (2) is internally provided with a second heating cavity (21), the upper part of the second heating cavity (21) is opened and is separated from the first heating cavity (11) through an electric partition board (5), the second heating cavity (21) is communicated with an external pressure regulating pipe (22), the external end of the pressure regulating pipe (22) is provided with an electric three-way valve (23), one of the other two ports of the electric three-way valve (23) is communicated to the first heating cavity (11) through a pipeline, and the other port is communicated with a heat exchange facility;
The electric three-way valve (23) and the electric partition plate (5) are electrically connected to the controller (4), and the controller (4) is configured to: controlling the microwave heating structure (1) to heat according to the d1 rate;
The controller (4) is configured to:
If the temperature of the raw materials in the first heating cavity (11) is normal temperature, the electric partition board (5) is controlled to be opened;
If the temperature in the heating cavity II (21) is higher than the temperature T1 and the temperature of the raw material in the heating cavity I (11) is lower than the normal temperature, controlling the electric three-way valve (23) to be communicated with the heating cavity I (11);
A steam pipeline (12) is arranged on the cavity wall of the heating cavity I (11) in a penetrating way, and the outer end of the steam pipeline (12) is communicated with a pipeline at the outer end of the electric three-way valve (23);
The electric partition plate (5) comprises a plurality of movable plates (51), a rotating shaft and a first motor (52), wherein the movable plates (51) are spliced to separate a first heating cavity (11) and a second heating cavity (21), the rotating shaft is fixed at one end, close to the cavity wall, of each movable plate (51), the rotating shaft is rotationally connected with the cavity wall of the first heating cavity (11), and the first motor (52) is used for driving the rotating shaft to rotate and is electrically connected with the controller (4);
The movable plate (51) is internally provided with a steam channel, at least one steam channel is provided with an air inlet which is in butt joint with a port in the steam pipeline (12) pair when the movable plate (51) is closed, the movable plate (51) is also provided with a plurality of steam holes, and the steam holes are upwards opened and downwards communicated with the steam channel.
2. The process for separating and purifying calpain according to claim 1, wherein: the heating equipment further comprises a stirring mechanism (6), a stirring part of the stirring mechanism (6) stretches into the first heating cavity (11) and is used for stirring raw materials on the movable plate (51), and the stirring mechanism (6) is electrically connected to the controller (4).
3. The separation and purification process of calpain according to claim 2, wherein: the stirring mechanism (6) comprises a stirring rod (61) and a motor II (62), the stirring rod (61) is rotatably connected to the cavity wall of the heating cavity I (11), the stirring rod (61) is hollow, and the temperature detection assembly (3) is used for detecting the temperature inside the stirring rod (61) and serving as the raw material temperature in the heating cavity I (11).
4. The process for separating and purifying calpain according to claim 1, wherein: the controller (4) is electrically connected with a pressure detection unit (7), a probe of the pressure detection unit (7) stretches into the heating cavity II (21), and the controller (4) is configured to:
If the heating cavity II (21) accords with a preset opening condition or the heating cavity II (21) accords with a preset pressure relief condition, the electric three-way valve (23) is controlled to relieve pressure according to the pressure value fed back by the pressure detection unit (7).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202411020610.8A CN118546197B (en) | 2024-07-29 | Separation and purification process of calpain |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202411020610.8A CN118546197B (en) | 2024-07-29 | Separation and purification process of calpain |
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| CN113813352A (en) * | 2021-10-08 | 2021-12-21 | 山东大学第二医院 | Calculus removing decoction for treating gallstone and preparation method thereof |
| CN117356735A (en) * | 2023-11-15 | 2024-01-09 | 成都木船食品科技有限公司 | Cooking system for sauce food processing and application method thereof |
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