CN118225537A

CN118225537A - Enrichment equipment

Info

Publication number: CN118225537A
Application number: CN202410545945.5A
Authority: CN
Inventors: 汤琳; 吴阿娜; 龚海燕; 怀红燕
Original assignee: Shanghai Environmental Monitoring Center (shanghai Yangtze River Delta Regional Air Quality Forecasting And Forecasting Center)
Current assignee: Shanghai Environmental Monitoring Center (shanghai Yangtze River Delta Regional Air Quality Forecasting And Forecasting Center)
Priority date: 2024-04-30
Filing date: 2024-04-30
Publication date: 2024-06-21

Abstract

The invention discloses an enrichment device, comprising: a gas supply assembly; the water sample storage part is used for containing water samples to be enriched, the water sample storage part is communicated with the air supply assembly, and the air supply assembly is used for providing protective gas for the water sample storage part; the speed regulating device is communicated with the water sample accommodating part and used for controlling the flow speed of the water sample; the first filter piece is communicated with the speed regulating device; the second filter piece, second filter piece and first filter piece intercommunication, the water sample of water sample holding portion loops through speed adjusting device, first filter piece, second filter piece for enrich the organic matter in the water sample to the second filter piece. The invention can accurately control the flow speed of the water sample, and can continuously enrich the water sample with large volume, so that the enriched organic matters have more quantity and less impurity.

Description

Enrichment equipment

Technical Field

The invention relates to the field of environmental monitoring, in particular to enrichment equipment.

Background

Along with the rapid development of economy and society and the continuous improvement of human living standard, the types and the quantity of organic matters entering into environmental water bodies are more and more, the number of the organic matters and artificially synthesized organic matters which are found at present is more than 500 tens of thousands, and the organic matters have the characteristics of durability, bioaccumulation, long-distance migration, toxic effect and the like, can enter various water bodies through different ways, even affect partial rivers and reservoirs which are taken as drinking water sources, and bring great harm to drinking water safety.

The Ames test (also called Bacterial Reverse Mutation Test and bacterial reverse mutation test) is a sensitive, quick and simple method for detecting DNA base damage by a compound with genetic toxicity, and is widely applied to the research of mutation caused by trace organic matters in water. The test utilizes salmonella typhimurium or escherichia coli auxotroph strains to be cultured together with detected organic matters, and if the detected organic matters have a certain mutability, the defective strains can be subjected to back mutation and then are mutated into wild type again. The wild-type strain itself can synthesize histidine/tryptophan, can grow and form colonies on histidine/tryptophan-free medium, and the auxotrophic strain itself cannot synthesize histidine/tryptophan, cannot form colonies on histidine/tryptophan-free medium, whereby mutability of the test substance can be evaluated.

However, most organic pollutants in the water body are extremely low in content and difficult to detect, the existing common organic matter enrichment methods at home and abroad are a resin adsorption separation method, a reverse osmosis separation method and an ultrafiltration separation method, the enrichment effect is affected to different degrees by the environmental conditions such as sediment, plankton and the like in the water body, the pore diameter, the material, the size and the like of a filtering column, the amount of the enriched water sample is different from a few milliliters to a few liters, components such as a buret and the like are required to be manually inserted in the enrichment process, the enrichment of a large-volume water sample cannot be performed, the enriched organic matters possibly contain impurities, and the quality is low.

At present, a system and a standard device for enriching organic matters in a large-volume water sample are not available, and the requirements of Ames test on test monitoring of a drinking water source and the like cannot be met.

Disclosure of Invention

The invention aims to solve the technical problem that high-quality enrichment cannot be carried out on a large-volume water sample. The invention provides enrichment equipment which can enrich a large-volume water sample to obtain enrichment samples with quality and quantity meeting requirements.

In order to solve the above technical problems, an embodiment of the present invention discloses an enrichment apparatus, including:

a gas supply assembly;

the water sample storage part is used for containing water samples to be enriched, the water sample storage part is communicated with the air supply assembly, and the air supply assembly is used for providing protective gas for the water sample storage part;

The speed regulating device is communicated with the water sample accommodating part and used for controlling the flow speed of the water sample;

the first filter piece is communicated with the speed regulating device;

The second filter piece, the second filter piece with first filter piece intercommunication, the water sample of water sample holding portion loops through speed adjusting device first filter piece, second filter piece is used for with organic matter enrichment in the water sample extremely the second filter piece.

By adopting the technical scheme, the protective gas can be provided for the water sample accommodating part through the gas supply assembly, so that the water sample flows into the speed regulating device, the flow speed of the water sample can be precisely controlled through the cooperation of the gas supply assembly and the speed regulating device, the residence time of the water sample in the second filter element is further regulated, organic matters in the water sample can be fully enriched in the second filter element, manual operation is not needed in the process, and the water sample with large volume can be continuously enriched, so that the organic matters obtained by enrichment are more and less in impurity.

According to another embodiment of the invention, an enrichment device is disclosed, wherein the water sample containing part comprises a first sealing part, the first sealing part comprises an inlet and an outlet, the inlet is used for the entry of the protective gas or the water sample, and the outlet is used for the outflow of the water sample.

By adopting the technical scheme, the sealing performance of the water sample accommodating part can be improved, on one hand, the water sample can be prevented from escaping from the water sample accommodating part, and on the other hand, the water sample can be prevented from being contacted with air.

According to another embodiment of the invention, the enrichment device comprises a water sample containing part and a second sealing part, wherein the first sealing part is arranged at the outlet, the second sealing part is arranged at the inlet, and the height of the first sealing part is higher than that of the second sealing part along the first direction.

By adopting the technical scheme, through setting up first sealing member and second sealing member, can further improve the sealing performance of water sample holding portion, in addition, the high of first sealing member is higher than the second sealing member, is convenient for distinguish import and export, avoids taking place the maloperation and causes the enrichment failure.

According to another embodiment of the invention, the water sample containing part is provided with one water sample enriching device, the inlet is communicated with the air supply assembly, the outlet is communicated with the speed regulating device, and the air supply assembly provides the protective gas for the water sample containing part through the inlet and is used for enabling the water sample to flow into the speed regulating device through the outlet.

By adopting the technical scheme, the water sample is stored through the water sample containing part, so that the cost can be reduced.

According to another specific embodiment of the invention, the embodiment of the invention discloses enrichment equipment, the number of the water sample accommodating parts is multiple, the water sample accommodating parts are sequentially communicated in series, and the air supply assembly is communicated with any water sample accommodating part.

By adopting the technical scheme, the same water quantity is stored in the plurality of water sample containing parts, so that the volume of each water sample containing part is smaller, and the sampling and the transportation are convenient.

According to another embodiment of the invention, an enrichment device is disclosed, wherein the water sample containing part comprises 60 liters of the water sample, the number of the plurality of water sample containing parts is 6, and each water sample containing part comprises 10 liters of the water sample.

By adopting the technical scheme, the 60L water sample is enriched, so that the organic matter quantity meeting the Ames test requirement can be obtained.

According to another specific embodiment of the invention, the embodiment of the invention discloses an enrichment device, the speed regulating device comprises an elastic tube and an extrusion part, one end of the elastic tube is communicated with the water sample accommodating part, the other end of the elastic tube is communicated with the first filtering piece, and the extrusion part can be switched between a first position and a second position;

In the first position, the extrusion part extrudes the elastic tube, the elastic tube is closed, and the water sample stops flowing into the first filter element; in the second position, the pressing part does not contact the elastic tube, the elastic tube is conducted, and the water sample flows into the first filter element.

By adopting the technical scheme, the water quantity of the water sample flowing into the first filter element can be controlled by controlling the frequency of the switching of the extrusion part between the first position and the second position, so that the flow speed of the water sample can be controlled, the water sample can stay in the second filter element for a longer time, and organic matters in the water sample are fully enriched to the second filter element.

According to another specific embodiment of the invention, the enrichment equipment is disclosed in an embodiment of the invention, the first filter piece comprises at least one first filter part and at least one quartz sand filter part, the first filter part is arranged at one end of the first filter piece, which is communicated with the speed regulating device, and is used for primarily filtering the water sample, and the quartz sand filter part is overlapped on the first filter part and is used for supporting the first filter part and secondarily filtering the primarily filtered water sample.

By adopting the technical scheme, on the one hand, because the first filtering part (such as quartz wool) is easy to extrude and deform under nitrogen pressure, the filtering efficiency is influenced, and therefore, the filtering efficiency is prevented from being influenced by overlapping the quartz sand filtering part on the first filtering part, and the first filtering part can be supported due to the fixed shape of the quartz sand. On the other hand, adopt quartz sand filter part and first filter part superimposed scheme, can enough guarantee that filtration efficiency is higher, can prevent again that first filter from blockking up.

According to another embodiment of the invention, the embodiment of the invention discloses an enrichment device, the second filter piece comprises a second filter part and a third filter part, the second filter piece comprises a water inlet end and a water outlet end, the water inlet end is communicated with the first filter piece, and the water outlet end is used for discharging waste liquid;

The second filtering part is arranged at the water inlet end and is used for filtering the water sample and preventing the water inlet end from being blocked, and the third filtering part is arranged at the water outlet end and is used for preventing the waste liquid from being sucked backwards and entering the second filtering part.

By adopting the technical scheme, the filtering effect can be further improved by arranging the second filtering part and the third filtering part, and the second filtering part is arranged at the water inlet end, so that the blockage of the water inlet end can be prevented, and the enrichment efficiency is influenced; the third filtering part is arranged at the water outlet end, so that waste liquid can be prevented from being sucked back into the second filtering part, and the large-aperture resin can be prevented from flowing out of the second filtering part, so that enrichment failure is caused.

According to another embodiment of the invention, the second filter element comprises a large-pore-diameter resin, and the large-pore-diameter resin is arranged between the second filter part and the third filter part and is used for enriching organic matters in the water sample.

According to another embodiment of the invention, an embodiment of the invention discloses an enrichment device, wherein the first filtering part, the second filtering part and the third filtering part are all filter cotton.

By adopting the technical scheme, the filter cotton is adopted as the filter medium, so that higher filter efficiency can be obtained.

According to another specific embodiment of the invention, the second filter element comprises a second sealing part, the enrichment device comprises an elastic fastener, the second sealing part is arranged at the water inlet end, the second sealing part comprises a fluid through hole and is used for supplying water sample or immersion liquid into the second filter element, and the elastic fastener is clamped with the second sealing part and the second filter element at the same time.

According to another embodiment of the present invention, an enrichment apparatus is disclosed in an embodiment of the present invention, where the elastic fastener includes a first portion, a second portion, and a third portion, the first portion and the second portion are spaced apart from each other along a first direction and disposed at two ends of the third portion, the second filter is clamped to the first portion, and the second seal is clamped to the second portion.

By adopting the technical scheme, the second sealing part is clamped to the second filter element through the elastic fastener, so that the second filter element can be ensured to have a good sealing effect, the second sealing part can be conveniently opened, and the second filter element is internally filled with filtering and adsorbing materials such as large-aperture resin.

According to another embodiment of the present invention, an embodiment of the present invention discloses an enrichment apparatus, wherein the ratio of the diameter to the length of the second filter element is 1: 4-1: 10, and the length of the second filter is not less than 10cm.

By adopting the technical scheme, the water sample can be fully contacted with the large-aperture resin in the second filter element, and the problem of blockage caused by too small diameter of the second filter element during the later-stage filtration of enrichment can be avoided.

According to another embodiment of the present invention, an enrichment apparatus is disclosed wherein the first filter member is in communication with the second filter member via a hose.

By adopting the technical scheme, compared with the fixed connection of the first filtering piece and the second filtering piece, the arrangement mode of the first filtering piece and the second filtering piece in the scheme is more flexible.

Drawings

Fig. 1 shows a schematic diagram of an enrichment device in some embodiments.

FIG. 2 shows an overall schematic of an enrichment apparatus provided by an embodiment of the present application.

Fig. 3 shows a top view of a cover of a water sample receiving portion of an enrichment apparatus according to an embodiment of the present application.

Fig. 4 shows an enlarged partial schematic view of a water sample receiving portion of an enrichment apparatus according to an embodiment of the present application.

FIG. 5 shows a front view of a cover of a water sample receiving portion of an enrichment apparatus according to an embodiment of the present application

FIG. 6 shows a partial schematic view of a water sample receiving portion of an enrichment apparatus according to an embodiment of the present application.

Fig. 7 shows a schematic diagram of a speed regulating device of an enrichment apparatus according to an embodiment of the present application.

FIG. 8 illustrates a partial cross-sectional view of a second filter element of an enrichment apparatus provided in an embodiment of the present application.

FIG. 9 shows a schematic view of a second seal of a second filter of an enrichment apparatus provided by an embodiment of the present application.

FIG. 10 illustrates a top view of a resilient fastener of an enrichment apparatus provided by an embodiment of the present application.

FIG. 11 illustrates a front view of a resilient fastener of an enrichment apparatus provided by an embodiment of the present application.

Detailed Description

Further advantages and effects of the present invention will become apparent to those skilled in the art from the disclosure of the present specification, by describing the embodiments of the present invention with specific examples. While the description of the invention will be described in connection with the preferred embodiments, it is not intended to limit the inventive features to the implementation. Rather, the purpose of the invention described in connection with the embodiments is to cover other alternatives or modifications, which may be extended by the claims based on the invention. The following description contains many specific details for the purpose of providing a thorough understanding of the present invention. The invention may be practiced without these specific details. Furthermore, some specific details are omitted from the description in order to avoid obscuring the invention. It should be noted that, without conflict, the embodiments of the present invention and features of the embodiments may be combined with each other.

It should be noted that in this specification, like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures.

In the description of the present embodiment, it should be noted that the azimuth or positional relationship indicated by the terms "upper", "lower", "inner", "bottom", etc. are based on the azimuth or positional relationship shown in the drawings, or the azimuth or positional relationship in which the inventive product is conventionally put in use, are merely for convenience of describing the present invention and simplifying the description, and are not indicative or implying that the apparatus or element to be referred to must have a specific azimuth, be configured and operated in a specific azimuth, and therefore should not be construed as limiting the present invention.

The terms "first," "second," and the like are used merely to distinguish between descriptions and are not to be construed as indicating or implying relative importance.

In the description of the present embodiment, it should also be noted that, unless explicitly specified and limited otherwise, the terms "disposed," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present embodiment can be understood in a specific case by those of ordinary skill in the art.

For the purpose of making the objects, technical solutions and advantages of the present invention more apparent, embodiments of the present invention will be described in further detail below with reference to the accompanying drawings.

In some embodiments, referring to fig. 1, the enrichment device includes a burette 100, a support frame 200, and a water sample barrel (not shown in the figure), the burette 100 is fixedly disposed on the support frame 200, the burette 100 includes a liquid inlet end 101 and a liquid outlet end 102, the liquid inlet end 101 is communicated with the water sample barrel, the liquid outlet end 102 is used for discharging waste liquid, the liquid outlet end 102 further includes a knob 1021 and a flow stop 1022, the knob 1021 is connected with the flow stop 1022, the flow stop 1022 is disposed through the burette 100, and the position of the flow stop 1022 in the burette 100 can be controlled by rotating the knob 1021 so as to adjust the speed of the water sample flowing out of the burette 100.

When enriching a water sample, a knob 1021 is required to be continuously rotated to obtain a relatively stable water sample outflow speed, when enriching a large volume of water sample, because the enrichment time of the large volume of water sample is long, for example, when enriching 60L of water sample, in order to obtain the requirement of Ames test, the enrichment time is usually at least more than 6 days, when enriching by using a buret 100, after an operator adjusts the position of a flow stop 1022, the outflow speed of the water sample at the liquid outlet end 102 is gradually slowed down or even stopped along with the increase of the enrichment time, so that the manual intervention is required to be performed for fine-adjusting the buret 100, so that the water sample can maintain the relatively stable outflow speed.

It can be seen that enriching a large volume of water sample by the above scheme includes at least the following disadvantages: ① The flowing speed of the water sample is unstable, and the enrichment effect is affected. ② In the enrichment process, an artificial non-timing intervention operation instrument is needed, and for enrichment of a large-volume water sample, a long time is needed, so that the artificial non-timing intervention operation instrument has great difficulty in practice, and therefore, as can be appreciated, some devices for enriching the water sample in the field are also difficult to be used for continuous enrichment of the large-volume water sample for a long time in the prior art, and an enrichment sample acquired by the device for enriching the water sample in the field also needs to be transported back to a laboratory, so that the storage difficulty of the enrichment sample is great.

Based on this, the present application provides, in some embodiments, an enrichment apparatus, referring to fig. 2, comprising a gas supply assembly 10, a speed regulating device 20, a first filter 30, a second filter 40, and at least one water sample receiving portion 50. The water sample accommodating part 50 is used for accommodating a water sample to be enriched, the water sample accommodating part 50 is communicated with the air supply assembly 10, and the air supply assembly 10 is used for providing protective gas for the water sample accommodating part 50; the speed regulating device 20 is communicated with the water sample accommodating part 50 and is used for controlling the flow speed of the water sample; the first filter element 30 is in communication with the speed regulating device 20; the second filter 40 is communicated with the first filter 30, and the water sample in the water sample accommodating portion 50 sequentially passes through the speed regulating device 20, the first filter 30 and the second filter 40, and is used for enriching organic matters in the water sample to the second filter 40. Illustratively, the air supply assembly 10, the speed regulating device 20, the first filter member 30, the second filter member 40 and the at least one water sample receiving portion 50 are all in communication via a hose 60, which may facilitate the placement of the foregoing components or devices within a laboratory. It will be appreciated that the foregoing components may also be in communication via PVC tubing, and the application is not limited in this regard.

By adopting the above technical scheme, the air supply assembly 10 can provide protective gas for the water sample accommodating part 50, so that the water sample flows into the speed regulating device 20, the air supply assembly 10 is matched with the speed regulating device 20, the flow speed of the water sample can be precisely controlled, the residence time of the water sample in the second filter 40 is further regulated, the organic matters in the water sample can be fully enriched in the second filter 40, and the process does not need manual operation, and can be used for continuously enriching a large volume of water sample so as to obtain an enriched sample with quality and quantity.

In some embodiments, the shielding gas provided by the gas supply assembly 10 is nitrogen, the gas supply assembly 10 includes a gas supply part 101 and a control part (not shown in the figure), the gas supply part 101 is connected with the control part, and the control part can adjust the pressure of the nitrogen gas filled into the water sample accommodating part 50 by the gas supply part 101 so as to control the rate of the water sample flowing into the water sample accommodating part 50. Illustratively, the control portion controls the output pressure of the nitrogen to be 0.1Mpa.

On the one hand, nitrogen is a highly stable protective gas, which can protect oxygen or moisture sensitive organic compounds in water body from unnecessary oxidation or hydrolysis reaction; on the other hand, when the water sample contacts with the nitrogen, the flow of the nitrogen can drive the volatile organic compounds escaping from the surface of the solution to enter the second filter 40, so that the filtering and enriching effects can be enhanced.

It will be appreciated that the shielding gas that can be provided by the gas supply assembly 10 may be other chemically stable gases such as argon, and the application is not limited in this regard.

In some embodiments, referring to fig. 3, 4, 5 in combination with fig. 2, the water sample receiving portion 50 includes a first sealing portion 501, the first sealing portion 501 including an inlet 5011 for the ingress of shielding gas or water sample and an outlet 5012 for the egress of water sample. Illustratively, the water sample receiving portion 50 includes a housing 502, the first sealing portion 501 includes a cover 5013 and a first protruding portion 5014, the first protruding portion 5014 protrudes from the housing 502 along a first direction X, the first protruding portion 5014 includes a first sealing ring 5015, and the first sealing ring 5015 is disposed on a side of the first protruding portion 5014 facing away from the housing 502 in the first direction X, and the first protruding portion 5014 and the cover 5013 are connected by threads. The inlet 5011 and the outlet 5012 are provided at intervals in the second direction Y to the cover 5013.

In some embodiments, the water sample receiving portion 50 includes a first seal 503 and a second seal 504, the first seal 503 is disposed at the outlet 5012, the second seal 504 is disposed at the inlet 5011, and the first seal 503 is higher than the second seal 504 along the first direction X. Illustratively, the first seal 503 includes a first threaded connection 5031, with one end of the first threaded connection 5031 in the first direction X communicating with the hose 60 and the other end threadably coupled to the inlet 5011 to effect a sealed securement of the hose 60 to the first seal 503. The second sealing member 504 includes a second threaded connection post 5041, and in the first direction X, one end of the second threaded connection post 5041 communicates with the hose 60, and the other end is in threaded connection with the outlet 5012, so that sealing fixation of the hose 60 to the second sealing member 504 is achieved, and the height of the first threaded connection post 5031 is higher than that of the second threaded connection post 5041, so that the inlet 5011 and the outlet 5012 can be distinguished.

Illustratively, the first seal 503 includes a second seal ring 5032 and a nut 5033, the second seal ring 5032 being disposed between the nut 5033 and the first threaded connection 5031. The second sealing member 504 includes a third sealing ring (not shown) provided between the outlet 5012 and the second screw connection post 5041 for enhancing the sealing performance of the first sealing member 503 and the second sealing member 504. It will be appreciated that the particular form of the first seal 503 and the second seal 504 are not fixed, for example, the second seal 504 may also include a nut 5033, the nut 5033 being disposed between the outlet 5012 and the second threaded post 5041.

Illustratively, the water sample receiving portion 50 is provided in a rectangular parallelepiped shape, and it is understood that the water sample receiving portion 50 may have other shapes such as a cylindrical shape.

In some embodiments, referring to fig. 3, 4, 6 in combination with fig. 2, the water sample receiving portion 50 includes an internal drain 505, the internal drain 505 extending in a first direction X, the internal drain 505 being in communication with the outlet 5012 for draining water sample from within the water sample receiving portion 50. In some embodiments, the first sealing portion 501 includes a metal pendant 5016, along the first direction X, the metal pendant 5016 is disposed on a side of the cover 5013 facing away from the first sealing member 503, and along the first direction X, the metal pendant 5016 is disposed on an end of the internal drain pipe 505 away from the cover 5013, for ensuring that the internal drain pipe 505 is always located at the bottom of the water sample receiving portion 50, so that the water sample can be discharged from the outlet 5012 under the pressure of the protective gas.

In some embodiments, to meet the requirements of the Ames test, the water sample receiving portion 50 provided by the present application comprises 60 liters of water sample in combination with 2 to 3 liters of water per day required by a normal person to sustain life. In some embodiments, the number of water sample receptacles 50 is one, inlet 5011 is in communication with air supply assembly 10, outlet 5012 is in communication with speed governor 20, and air supply assembly 10 provides shielding gas to water sample receptacles 50 through inlet 5011 for flowing water sample into speed governor 20 through outlet 5012. In some embodiments, the number of water sample containers 50 is plural, and the plural water sample containers 50 are sequentially connected in series, and the air supply assembly 10 is connected to any one water sample container 50. For example, the number of the water sample containers 50 is 6, each water sample container 50 includes 10 liters of water sample, the inlet 5011 of any one water sample container 50 is communicated with the air supply assembly 10, and the inlets 5011 of the other water sample containers 50 are sequentially communicated with the outlet 5012 of the previous water sample container 50 for supplying water sample into the water sample containers 50 connected in series.

It will be appreciated that the water sample receiving portion 50 may include water samples with small volumes of 1 liter, 2 liters, 3 liters, 4 liters, etc., water samples with large volumes of 50 liters, 70 liters, 80 liters, etc., and the number of water sample receiving portions 50 may be 2,3, 4,5, 7, 8, etc., which is not limited in this application.

In some embodiments, referring to fig. 7, the speed regulating device 20 includes an elastic tube 201 and an extrusion part 202, wherein one end of the elastic tube 201 is communicated with the water sample accommodating part 50, the other end is communicated with the first filter 30, and the extrusion part 202 can be switched between a first position and a second position; in the first position, the pressing part 202 presses the elastic tube 201, the elastic tube 201 is closed, and the water sample stops flowing into the first filter 30; in the second position, the squeeze portion 202 does not contact the elastic tube 201, the elastic tube 201 is conducted, and the water sample flows into the first filter 30. Illustratively, the governor device 20 includes a housing 203, the housing 203 including a receiving groove 2031, the squeeze portion 202 being disposed within the receiving groove 2031, and the elastic tube 201 being disposed between the squeeze portion 202 and the receiving groove wall. Illustratively, the pressing portion 202 includes a driving rod 2021 and a roller 2022, the driving rod 2021 is capable of rotating along a circumferential direction R under the action of a motor (not shown in the drawings), the roller 2022 is disposed at one end of the driving rod 2021, and the elastic tube 201 is disposed between the roller 2022 and the accommodating groove wall.

When the driving lever 2021 is rotated to the first position a, the roller 2022 presses the elastic tube 201, and the elastic tube 201 is pressed to form a closed cut-off point, thereby forming a negative pressure in the elastic tube 201, and sucking the liquid. When the driving rod 2021 continues to rotate to the second position b, the roller 2022 leaves the elastic tube 201, and the elastic tube 201 is rebounded to be in a conducting state, so that the water sample flows out of the speed regulating device 20, and the flowing direction of the water sample is shown by an arrow at c in the figure.

By changing the frequency of switching the squeeze portion 202 between the first position a and the second position b, the elastic tube 201 can be continuously switched between the on and off states, thereby adjusting the speed at which the water sample flows out of the speed regulating device 20. The speed regulating device is a peristaltic pump, and the air supply pressure of the air supply assembly is adjusted to be matched with the peristaltic pump, so that the flow rate of the water sample is about 30-40 milliliters per minute.

In some embodiments, referring to fig. 2, the first filter 30 includes at least one first filter 301 and at least one quartz sand filter 302, the first filter 301 is disposed at an end of the first filter 30 in communication with the speed regulator 20, for performing primary filtration on the water sample, and the quartz sand filter 302 is stacked on the first filter 301, for supporting the first filter 301 and performing secondary filtration on the primary filtered water sample. Illustratively, the first filter element 30 is cylindrically disposed, and the first filter element 30 includes two first filter portions 301 and two quartz sand filter portions 302 therein, wherein, along the first direction X (i.e., the height direction of the first filter element 30), each first filter portion 301 and each quartz sand filter portion 302 are adjacently disposed, and the quartz sand filter portions 302 can perform the function of removing impurities and silt in the water sample, on the one hand, and on the other hand, the quartz sand has a fixed shape, and can also perform the supporting function on the first filter portion 301, so as to avoid the first filter portion 301 from being deformed by extrusion under the nitrogen pressure, and affect the filtering efficiency. Illustratively, the first filter element 30 includes a column of 8-16 mesh quartz sand contained therein.

In some embodiments, referring to fig. 8 in combination with fig. 2, the second filter element 40 includes a second filter portion 401 and a third filter portion 402, the second filter element 40 includes a water inlet end 403 and a water outlet end 404, the water inlet end 403 is in communication with the first filter element 30, and the water outlet end 404 is for discharging waste liquid; the second filtering portion 401 is disposed at the water inlet end 403, and is used for filtering water sample and preventing the water inlet end 403 from being blocked, and the third filtering portion 402 is disposed at the water outlet end 404, and is used for preventing waste liquid from being sucked back into the second filtering member 40, resulting in enrichment failure. The second filter element 40 is illustratively provided in a cylindrical shape, and it is understood that the first filter element 30 and the second filter element 40 may have other shapes such as a rectangular parallelepiped shape.

In some embodiments, referring to fig. 8 in combination with fig. 2, the second filter 40 comprises a large pore size resin 405, the large pore size resin 405 being disposed between the second filter 401 and the third filter 402 for enriching the organic matter in the water sample. The large-pore resin 405 is a resin with a pore diameter of 10-500 μm, and has strong adsorption and permeation capabilities to macromolecules, and in addition, the large-pore resin 405 has a large surface area, can improve adsorption efficiency and resolution, and has a longer service life than a common resin due to the stable structure of the large-pore resin 405. Illustratively, 30ml of activated macroporous resin 405 is provided in the second filter element 40, and the activation of the macroporous resin 405 means that the macroporous resin 405 is rinsed by pure water and methanol, and is soaked in an organic solvent (methanol, dichloromethane, cyclohexane and acetonitrile) in a Soxhlet extractor for 8 to 24 hours to remove organic matters, thereby completing the activation. The activated resin is soaked in methanol for standby, and the second filter element is washed with distilled water for 3 to 4 times before use.

Illustratively, the first filter 301, the second filter 401, and the third filter 402 are all filter cotton. It will be appreciated that the filter cotton may comprise quartz filter cotton, glass fiber filter cotton, etc., and the application is not limited to the specific materials of the filter cotton.

In some embodiments, referring to fig. 8, 9 in combination with fig. 2, the second filter 40 includes a second sealing portion 406, the second sealing portion 406 being disposed at the water inlet end 403, the second sealing portion 406 including a fluid through hole 4061 for supplying water sample or a rinse solution into the second filter 40. Illustratively, the second sealing portion 406 includes a first connecting portion 4062 and a second protruding portion 4063, the first connecting portion 4062 extends along the second direction Y, the fluid through hole 4061 is disposed in the first connecting portion 4062 and the second protruding portion 4063, the second protruding portion 4063 protrudes in the first direction X and is disposed in the second filter element 40, and a fourth sealing ring (not shown) is disposed at an end of the first connecting portion 4062 away from the second protruding portion 4063, and the first connecting portion 4062 abuts against the water inlet end 403.

In some embodiments, referring to fig. 8, 10, 11 in combination with fig. 2, the enrichment apparatus includes a resilient fastener 70, the resilient fastener 70 being simultaneously snapped into engagement with the second seal 406 and the second filter 40 for connecting the second seal 406 to the water inlet end 403. In some embodiments, the elastic fastener 70 includes a first portion 701, a second portion 702, and a third portion 703, where the first portion 701 and the second portion 702 are spaced apart from each other along the first direction X and disposed at two ends of the third portion 703, the second filter 40 is clamped to the first portion 701, and the second sealing portion 406 is clamped to the second portion 702. Illustratively, the first portion 701, the second portion 702, and the third portion 703 are all arc-shaped, the arc-shaped side wall 7011 of the first portion 701 can be disposed in a fitting manner with the side surface of the second filter element 40, the third portion 703 includes an arc-shaped upper surface 7031 and an arc-shaped lower surface 7032 that are disposed opposite to each other along the first direction X, and the second protrusion 4063 abuts against the arc-shaped lower surface 7032 of the third portion 703.

As an example, referring to fig. 10 and 11, in the second direction Y, the second portion 702 is disposed on one side of the first portion 701 and the third portion 703, the one side of the first portion 701 facing away from the second portion 702 includes a plurality of concave portions 704 and a plurality of convex portions 705 disposed at intervals along the circumferential direction R, each concave portion 704 and each convex portion 705 are disposed adjacent, and the one side of the third portion 703 facing away from the second portion 702 also includes a plurality of concave portions 704 and a plurality of convex portions 705 disposed at intervals along the circumferential direction R, each concave portion 704 and each convex portion 705 are disposed adjacent.

In some embodiments, the ratio of the diameter to the length of the second filter element 40 is 1:4 to 1:10, and the length of the second filter 40 is not less than 10cm, so that the water sample is fully contacted with the large-pore-diameter resin 405 in the second filter 40 to obtain better enrichment effect. Illustratively, the ratio of the diameter to the length of the second filter element 40 may be 1: 5. 1: 6. 1: 7. 1: 8. 1:9, the application is not limited in this regard.

In some embodiments, after enrichment, the organics adsorbed on the large pore size resin are rinsed with 100 ml of 30% acetone-methanol solution for 10min, then trickled slowly, the eluate collected in sterile Erlenmeyer flasks, and transferred to evaporation dishes for evaporation and drying in a 45 ℃ water bath, and dissolved in an appropriate amount of Dimethylsulfoxide (DMSO) for use.

In order to fully illustrate that the enrichment device provided by the embodiment of the application has a better enrichment effect, the application will be described with reference to the example data of the Ames test.

TABLE 1 results of enrichment of TA98 strain Ames in water sample

As shown in table 1, in some embodiments, a blank group, a control group and a test group are provided, wherein the blank group is to culture the TA98 strain in the culture medium by taking dimethyl sulfoxide as a solvent as a sample, the control group is to enrich the supplied water sample through related equipment in the prior art and then to culture the TA98 strain in the culture medium, and the test group is to enrich the supplied water sample through the enrichment equipment provided by the application and then to culture the TA98 strain in the culture medium.

The TA98 strain can be used for detecting a frameshift mutagen, and the mutagen is also called a mutagen, and can enable the histidine-deficient mutant of salmonella typhimurium to be subjected to back mutation into a wild type, so that whether a test object is the mutagen can be judged according to the number of colonies generated on a culture medium, and an S9 mixed solution+TA 98 strain can be used for testing an indirect mutagen. It will be appreciated that the application is not limited to a particular strain type, and that other strains, such as TA100 strain, TA102 strain, TA97 strain, TA1535 strain, etc., may alternatively be tested in culture.

Illustratively, SD in table 1 represents the standard deviation value of the data obtained from the three trials, and the lowest mutation amount is the sample dose that causes 2-fold of the number of the reverted colonies of the blank group when the test object exhibits a positive reaction (the number of reverted colonies per dish is equal to or greater than 2-fold of the number of reverted colonies of the blank group and has a dose-response relationship). The blank group refers to three parallel culture tests of TA98 strain when the inoculation amount of dimethyl sulfoxide is 0.1ml, and the obtained strain numbers on the culture medium are respectively as follows: the average of 26, 32 and 27 strains was 28.3 strains, and it can be understood that three parallel culture tests were also performed on the TA98 strain+s9 mixed solution, and the strains on the obtained culture medium were respectively: 41, 40 and 44, and the average value of the three tests is 41.7 strains.

When the number of TA98 bacteria plants in the control group or the test group is greater than or equal to 56.6, and the dose-response relationship is shown among the gradients of different sample water samples, the water samples are detected to be positive, and the minimum mutation quantity under the condition of the TA98 strain is calculated according to the dose-response relationship. When the number of strains in the TA98 strain+S9 mixed solution in the control group or the test group is more than or equal to 83.4, and the dose-response relationship is shown among gradients of different sample water samples, the water samples are detected to be positive, and the lowest mutation quantity in the TA98 strain+S9 mixed solution is calculated according to the dose-response relationship.

The control group and the test group of the embodiment of the application are tested by adopting gradient water sample amounts of 0.25L, 0.5L, 1L and 2L, and the linear relation corresponding to the colony number and the water sample amount can be obtained according to the obtained data, and the related linear relation can be known: the lowest mutation amount in the control group is 1.417L, namely when the water sample amount is 1.417L, the number of bacterial plants in the control group is 56.6; the minimum mutation amount in the test group was 0.738L, i.e., the number of bacterial plants in the control group was 56.6 when the water sample amount was 0.738L. Compared with the related equipment in the prior art, the enrichment equipment provided by the application has better enrichment effect under the same condition, namely, the enrichment equipment can enrich more organic matters, so that the minimum mutation quantity is lower.

In addition, under the condition of the TA98 strain+S9 mixed solution, no positive is detected in the water sample enriched by the prior art, and after the same water sample is enriched by the enrichment equipment provided by the application, the positive is detected and the minimum mutation amount is 1.504L. Therefore, the enrichment device provided by the application has better enrichment effect, and the enriched water sample can obtain more accurate results when being used for Ames test.

While the invention has been shown and described with reference to certain preferred embodiments thereof, it will be understood by those skilled in the art that the foregoing is a further detailed description of the invention with reference to specific embodiments, and it is not intended to limit the practice of the invention to those descriptions. Various changes in form and detail may be made therein by those skilled in the art, including a few simple inferences or alternatives, without departing from the spirit and scope of the present invention.

Claims

1. An enrichment device, comprising:

a gas supply assembly;

the first filter piece is communicated with the speed regulating device;

2. An enrichment apparatus according to claim 1, wherein the water sample receiving portion comprises a first sealing portion comprising an inlet for the ingress of the shielding gas or the water sample and an outlet for the egress of the water sample.

3. An enrichment apparatus according to claim 2, wherein the water sample receiving portion comprises a first seal and a second seal, the first seal being provided at the outlet and the second seal being provided at the inlet, the first seal being higher in height than the second seal in a first direction.

4. An enrichment apparatus according to claim 3, wherein the number of water sample receptacles is one, the inlet is in communication with the gas supply assembly, the outlet is in communication with the speed regulating means, and the gas supply assembly provides the shielding gas to the water sample receptacles through the inlet for flowing the water sample into the speed regulating means through the outlet.

5. An enrichment apparatus according to claim 3, wherein the number of water sample receptacles is plural, the plural water sample receptacles are in serial communication in sequence, and the air supply assembly is in communication with any one of the water sample receptacles.

6. An enrichment apparatus according to claim 5, wherein said water sample receptacles comprise 60 liters of said water sample, and said plurality of water sample receptacles is 6 in number, each of said water sample receptacles comprising 10 liters of said water sample.

7. The enrichment apparatus of any of claims 1-6, wherein the speed regulating device comprises an elastic tube and an extrusion part, one end of the elastic tube is communicated with the water sample accommodating part, the other end of the elastic tube is communicated with the first filter element, and the extrusion part can be switched between a first position and a second position;

8. The enrichment apparatus as claimed in any of claims 1-6, wherein the first filter element comprises at least one first filter part and at least one quartz sand filter part, the first filter part is arranged at one end of the first filter element, which is communicated with the speed regulating device, and is used for primarily filtering the water sample, and the quartz sand filter part is overlapped on the first filter part and is used for supporting the first filter part and secondarily filtering the primarily filtered water sample.

9. The enrichment apparatus of claim 1, wherein the second filter element comprises a second filter portion and a third filter portion, the second filter element comprising a water inlet end and a water outlet end, the water inlet end in communication with the first filter element, the water outlet end for discharging spent liquor;

10. An enrichment apparatus according to claim 9, wherein said second filter comprises a macroporous resin disposed between said second filter portion and said third filter portion for enriching organics in said water sample.

11. The enrichment apparatus of claim 9, wherein the first filter portion, the second filter portion, and the third filter portion are all filter cotton.

12. The enrichment apparatus of claim 9, wherein the second filter comprises a second seal, the enrichment apparatus comprising an elastic fastener, the second seal being disposed at the water inlet end, the second seal comprising a fluid through-hole for water sample or rinse solution to enter the second filter, the elastic fastener being simultaneously engaged with the second seal and the second filter.

13. The enrichment apparatus of claim 12, wherein the elastic fastener comprises a first portion, a second portion, and a third portion, the first portion being spaced apart from the second portion along a first direction at both ends of the third portion, the second filter being engaged with the first portion, the second seal being engaged with the second portion.

14. The enrichment apparatus of claim 1, wherein the second filter is cylindrical and the ratio of the diameter to the length of the second filter is 1: 4-1: 10, and the length of the second filter is not less than 10cm.

15. The enrichment apparatus of claim 1, wherein the first filter is in communication with the second filter via a hose.