CN118078695A - Composition with transdermal absorption promoting effect - Google Patents

Abstract

The present invention relates to a composition having the effect of promoting transdermal absorption, and belongs to the technical field of cosmetics. The composition comprises the following raw materials: volatile oil of cyperus rotundus, volatile oil of vitex rotundus, volatile oil of leucophylla, extract of kudzu root, extract of lithospermum officinale, extract of flos centellae, extract of centella asiatica and solvent. The present invention compounds volatile oils of three plant raw materials, volatile oil of cyperus rotundus, volatile oil of vitex rotundus and volatile oil of leucophylla, to accelerate penetration, increase membrane fluidity and promote deep diffusion of components, so as to promote transdermal absorption, and the composition has high safety, low skin irritation, and the transdermal absorption effect under the compounding of the raw materials has a broad spectrum; in addition, the present invention also uses kudzu root extract as the main ingredient, lithospermum officinale extract and flos centellae extract as the auxiliary ingredients, so that the prepared composition has excellent antibacterial and anti-inflammatory effects.

Description

A composition having the effect of promoting transdermal absorption

Technical Field

The invention belongs to the technical field of cosmetics, and in particular relates to a composition having the effect of promoting transdermal absorption.

Background technique

Transdermal absorption in cosmetics refers to the process in which the functional ingredients in cosmetics act on the skin surface or enter the epidermis or dermis, and accumulate and play a role in this part. With the development of the cosmetics industry, people have higher and higher requirements for the functions of cosmetics. Many people hope to have a cosmetic that can both inhibit bacteria and reduce inflammation and promote transdermal absorption. When cosmetics have antibacterial ingredients, these ingredients can prevent bacteria from multiplying on the skin to a certain extent, thereby reducing bacterial damage to the skin. At the same time, since the bacteria on the skin surface are suppressed, its transdermal absorption channel is also more unobstructed, making it easier for active ingredients to penetrate into the deep layers of the skin. However, most of the cosmetics currently on the market focus on the efficacy of the finished product and ignore the issue of whether the user's skin can absorb it. Therefore, it is very necessary to develop a composition for cosmetics that has the effect of promoting transdermal absorption and antibacterial effects to meet consumers' demand for high-quality cosmetics.

Summary of the invention

The purpose of the present invention is to provide a composition with the effect of promoting transdermal absorption, which solves the problem that the compositions in the prior art have poor effects of promoting transdermal absorption and inhibiting bacteria.

The purpose of the present invention can be achieved through the following technical solutions:

A composition having the effect of promoting transdermal absorption, the composition comprising the following raw materials in parts by weight:

Cyperus rotundus is the dried rhizome of Cyperus rotundus, a plant of the genus Cyperus of the family Cyperaceae. Cyperus rotundus mainly contains volatile oil components, mainly cyperol, copaene, α-cyperone, cyperene, etc., and the rest are flavonoids and their glycosides and triterpenoid compounds. Cyperus rotundus volatile oil can effectively destroy the lipid structure of the stratum corneum, increase the diffusion coefficient of the components between cells, and thus accelerate the faster penetration of the effective components in the system;

Vitex rotundifolia is the dried mature fruit of Vitex unifolia or Vitex rotundifolia of the Verbenaceae family. Vitex rotundifolia contains volatile oil, the main components of which are pinene and camphene. Vitex rotundifolia volatile oil can effectively affect the balance of calcium ions inside and outside the membrane, increase membrane fluidity, and help make the active ingredients easier to penetrate the skin.

Ledum palustris is an evergreen upright shrub of the genus Ericaceae. The main components of Ledum palustris volatile oil are sabinene, p-cymene, 4-terpineol and cumaldehyde. Ledum palustris volatile oil can effectively improve the solubility parameters of the ingredients in the skin, thereby allowing the ingredients to diffuse quickly into the deep layers of the skin.

As a preferred technical solution of the present invention, the preparation method of the Cyperus rotundus volatile oil is: crushing the Cyperus rotundus raw material, sieving to obtain Cyperus rotundus powder, mixing the Cyperus rotundus powder and petroleum ether evenly and performing temperature-controlled ultrasonic extraction to obtain an extract, filtering to obtain a filtrate, and evaporating the filtrate to obtain the Cyperus rotundus volatile oil.

As a preferred technical solution of the present invention, the screening is through a 10-20 mesh sieve; the dosage ratio of the Cyperus rotundus powder and petroleum ether is 1g:10-15mL; the temperature-controlled ultrasonic extraction is specifically to control the temperature at 40-55°C and ultrasonic extraction at 180-200W, 40-45kHz for 35-40min; the filtration is to filter with a 0.45μm microporous filter membrane; the temperature-controlled evaporation is to control the temperature at 65-68°C and evaporate in a constant temperature water bath.

As a preferred technical solution of the present invention, the preparation method of the vitex rotundifolia volatile oil is as follows: crushing the vitex rotundifolia raw material, sieving to obtain vitex rotundifolia powder, mixing the vitex rotundifolia powder and deionized water evenly and heating to boiling, extracting after boiling to obtain an intermediate volatile oil, washing an extractor with petroleum ether, and mixing the washing liquid and the intermediate volatile oil evenly to obtain a mixed solution, adding excess anhydrous sodium sulfate to the mixed solution, standing and centrifuging, taking the supernatant and exposing it to air to evaporate the petroleum ether and filtering to obtain the vitex rotundifolia volatile oil.

As a preferred technical solution of the present invention, the screening is through a 10-20 mesh sieve; the usage ratio of the Vitex rotundifolia powder and deionized water is 1g:5-6mL; the extraction is continuous extraction in the extractor for 6-7h; the petroleum ether washing extractor is washing the extractor with a small amount of petroleum ether for 2-3 times to fully dissolve the volatile oil in the petroleum ether; the static centrifugation is centrifugation after static standing for 0.5-1h; the filtration is filtration using a 0.45μm microporous filter membrane.

As a preferred technical solution of the present invention, the preparation method of the Ledum odoratum volatile oil is as follows: the Ledum odoratum raw material is crushed and sieved to obtain Ledum odoratum powder, the Ledum odoratum powder and ethanol solution are evenly mixed and ultrasonically extracted at a controlled temperature to obtain an extract, filtered to obtain a filtrate, and evaporated to dryness at a controlled temperature to obtain the Cyperus rotundus volatile oil.

As a preferred technical solution of the present invention, the sieving is through a 10-20 mesh sieve; the dosage ratio of the Ledum powder and the ethanol solution is 1g:15-17mL; the temperature-controlled ultrasonic extraction is specifically controlled at 55-60°C for ultrasonic extraction at 200-210W and 45-50kHz for 15-20min; the filtration is filtered through a 0.45μm microporous filter membrane; and the temperature-controlled evaporation is controlled at 65-68°C for evaporation in a constant temperature water bath.

As a preferred technical solution of the present invention, the ethanol solution is an ethanol solution with a volume fraction of 50%.

As a preferred technical solution of the present invention, the kudzu root extract is obtained by extracting the dried roots of the leguminous plant kudzu with 50% alcohol by volume as the extraction solvent, and the ratio of kudzu root to alcohol is 1g:10mL through a conventional room temperature immersion method; the lithospermum root extract is obtained by extracting the dried roots of the Boraginaceae plant Xinjiang lithospermum with 30% alcohol by volume as the extraction solvent, and the ratio of lithospermum root to alcohol is 1g:10mL through a conventional room temperature immersion method; the centella asiatica extract is obtained by extracting the whole centella asiatica with 50% alcohol by volume as the extraction solvent, and the ratio of centella asiatica to alcohol is 1g:10mL through a conventional room temperature immersion method; the centella asiatica extract is obtained by extracting the dried whole centella asiatica with 50% alcohol by volume as the extraction solvent, and the ratio of centella asiatica to alcohol is 1g:10mL through a conventional room temperature immersion method.

As a preferred technical solution of the present invention, the solvent includes any one of ethanol, propylene glycol, glycerol, isopropanol and n-butanol.

A method for preparing a composition having the effect of promoting transdermal absorption, the method for preparing the composition specifically comprising the following steps:

The volatile oil of Cyperus rotundus, the volatile oil of Vitex rotundus, the volatile oil of Ledum palustris and the solvent are stirred evenly under temperature control, and the temperature is maintained, while adding the kudzu root extract, the lithospermum root extract, the flos chrysantha extract and the centella asiatica extract, stirring to obtain a finished composition.

As a preferred technical solution of the present invention, the temperature for temperature-controlled uniform stirring is 25-28° C.; and the stirring time is 1-1.5 h.

The invention discloses an application of a composition having the effect of promoting transdermal absorption. The composition having the effect of promoting transdermal absorption is added into skin care products at a mass percentage of 3-5%.

Beneficial effects of the present invention:

(1) The present invention compounds plant raw material volatile oils to promote transdermal absorption, which has high safety and low skin irritation, and the transdermal absorption effect of the raw material compound has a broad spectrum; specifically, the volatile oil of Cyperus rotundus destroys the lipid structure of the stratum corneum and increases the diffusion coefficient of the components between cells, thereby accelerating the faster penetration of the effective components in the system; the volatile oil of Vitex rotundus affects the calcium ion balance inside and outside the membrane and increases the membrane fluidity, which helps to make the effective components easier to penetrate the skin; the volatile oil of Ledum palustris increases the solubility parameters of the components in the skin, thereby allowing the components to diffuse quickly into the deep layers of the skin; that is, the volatile oil of Cyperus rotundus, the volatile oil of Vitex rotundus and the volatile oil of Ledum palustris accelerate the penetration, increase the membrane fluidity and promote the deep diffusion of the components, thereby synergistically improving the transdermal absorption effect of the system.

(2) The present invention unexpectedly discovered during the research and development process that the Vitex volatile oil and Ledum odoratum volatile oil added in the present invention have a good penetration-promoting effect on the Pueraria root extract, and can effectively enhance the anti-inflammatory effect of the system. Preliminary judgment is that the pinene component in the Vitex volatile oil and the pinolene component in the Ledum odoratum volatile oil can interact with the puerarin in the Pueraria root isoflavones of the Pueraria root extract, so that the Pueraria root extract can exert its antibacterial and anti-inflammatory effects to the greatest extent;

The present invention has conducted multiple studies on the extraction methods of various plant raw materials, and finally confirmed that Cyperus rotundus, Vitex rotundus and Ledum palustris are added as raw materials in the form of volatile oils, while Pueraria lobata, Lithospermum officinale and Centella asiatica are added in the form of conventional powdered extracts, and the volatile oils can also produce a synergistic effect with the extracts, thereby maximizing the characteristics of each raw material and strengthening the overall effect of the system.

(3) The present invention mainly uses kudzu root extract, and lithospermum root extract and centaury flower extract as supplementary ingredients, so that the prepared composition has excellent antibacterial and anti-inflammatory effects. In this system, the kudzu root extract with kudzu root isoflavone as the main active ingredient effectively inhibits the activation of NF-κB cells, thereby achieving anti-inflammatory effects; at the same time, the lithospermum root extract is supplemented, and the main active ingredients in the lithospermum root are naphthoquinone compounds such as shikonin, deoxyshikonin, acetylshikonin and isobutyrylshikonin, among which naphthoquinone derivatives such as shikonin have inhibitory effects on various fungi, and the centaury flower extract mainly contains bitter substances, alkaloids, phytosterols and phenolic acid compounds, which can effectively enhance the antibacterial and anti-inflammatory effects of the system.

(4) The present invention also adds a Centella asiatica extract containing ingredients such as asiatica glycoside, hydroxy-madecassoside, and asiatica sugar, which not only increases the antibacterial and anti-inflammatory effects of the composition but also adds anti-allergic and soothing effects, thereby enhancing the efficacy of the composition.

Detailed ways

The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

The kudzu root extract used in the present invention is obtained by extracting the dried roots of the leguminous plant kudzu with 50% alcohol by volume as an extraction solvent, and the ratio of kudzu root to alcohol is 1g:10mL through a conventional room temperature immersion method; the lithospermum root extract is obtained by extracting the dried roots of the boraginaceae plant Xinjiang lithospermum with 30% alcohol by volume as an extraction solvent, and the ratio of lithospermum root to alcohol is 1g:10mL through a conventional room temperature immersion method; the centella asiatica extract is obtained by extracting the whole centella asiatica with 50% alcohol by volume as an extraction solvent, and the ratio of centella asiatica to alcohol is 1g:10mL through a conventional room temperature immersion method; the centella asiatica extract is obtained by extracting the dried whole centella asiatica with 50% alcohol by volume as an extraction solvent, and the ratio of centella asiatica to alcohol is 1g:10mL through a conventional room temperature immersion method; the above will not be repeated in the following.

Example 1

A composition having the effect of promoting transdermal absorption, the composition comprising the following raw materials in parts by weight:

The preparation method of the volatile oil of Cyperus rotundus is as follows: crushing the raw material of Cyperus rotundus, passing it through a 10-mesh sieve to obtain Cyperus rotundus powder, mixing the Cyperus rotundus powder and petroleum ether in a dosage ratio of 1 g:13 mL, controlling the temperature at 40°C, and ultrasonically extracting for 38 minutes under the conditions of 190W and 40kHz to obtain an extract, filtering the extract with a 0.45 μm microporous filter membrane to obtain a filtrate, controlling the temperature of the filtrate at 65°C and evaporating it in a constant temperature water bath to obtain the volatile oil of Cyperus rotundus;

The preparation method of the vitex rotundifolia volatile oil comprises the following steps: crushing the vitex rotundifolia raw material, passing through a 20-mesh sieve to obtain vitex rotundifolia powder, uniformly mixing the vitex rotundifolia powder and deionized water at a dosage ratio of 1 g:5 mL and heating to boiling, and continuously extracting in an extractor for 6 hours after boiling to obtain an intermediate volatile oil, washing the inner wall of the extractor with a small amount of petroleum ether for 3 times, and uniformly mixing the washing liquid and the intermediate volatile oil to obtain a mixed solution, adding an excess of anhydrous sodium sulfate to the mixed solution, standing for 0.5 hours and then centrifuging, taking the supernatant and placing it in the air to evaporate the petroleum ether and filtering it with a 0.45 μm microporous filter membrane to obtain the vitex rotundifolia volatile oil;

The preparation method of the Ledum odoratum volatile oil comprises the following steps: crushing the Ledum odoratum raw material, passing it through a 10-mesh sieve to obtain Ledum odoratum powder, uniformly mixing the Ledum odoratum powder and an ethanol solution with a volume fraction of 50% at a dosage ratio of 1 g:16 mL, controlling the temperature at 55° C. and performing ultrasonic extraction for 15 minutes under the conditions of 205 W and 45 kHz to obtain an extract, filtering the extract with a 0.45 μm microporous filter membrane to obtain a filtrate, controlling the temperature of the filtrate at 68° C. and evaporating the filtrate in a constant temperature water bath to obtain the Cyperus rotundus volatile oil.

The preparation method of the composition having the effect of promoting transdermal absorption specifically comprises the following steps:

The volatile oil of Cyperus rotundus, the volatile oil of Vitex rotundus, the volatile oil of Ledum palustris and ethanol were stirred evenly at 27°C, and the temperature was maintained. The kudzu root extract, the lithospermum root extract, the flos centellae extract and the centella asiatica extract were added while stirring for 1 hour to obtain the finished composition.

Example 2

A composition having the effect of promoting transdermal absorption, the composition comprising the following raw materials in parts by weight:

The preparation method of the volatile oil of Cyperus rotundus is as follows: crushing the raw material of Cyperus rotundus, passing it through a 20-mesh sieve to obtain Cyperus rotundus powder, mixing the Cyperus rotundus powder and petroleum ether in a dosage ratio of 1 g:10 mL, controlling the temperature at 55° C., and ultrasonically extracting for 40 minutes under the conditions of 200 W and 43 kHz to obtain an extract, filtering the extract with a 0.45 μm microporous filter membrane to obtain a filtrate, controlling the temperature of the filtrate at 68° C. and evaporating it in a constant temperature water bath to obtain the volatile oil of Cyperus rotundus;

The preparation method of the vitex rotundifolia volatile oil comprises the following steps: crushing the vitex rotundifolia raw material, passing through a 10-mesh sieve to obtain vitex rotundifolia powder, uniformly mixing the vitex rotundifolia powder and deionized water at a dosage ratio of 1 g:6 mL and heating to boiling, and continuously extracting in an extractor for 7 hours after boiling to obtain an intermediate volatile oil, washing the inner wall of the extractor twice with a small amount of petroleum ether, and uniformly mixing the washing liquid and the intermediate volatile oil to obtain a mixed solution, adding an excess of anhydrous sodium sulfate to the mixed solution, standing for 1 hour and then centrifuging, taking the supernatant and placing it in the air to evaporate the petroleum ether, and filtering with a 0.45 μm microporous filter membrane to obtain the vitex rotundifolia volatile oil;

The preparation method of the Ledum odoratum volatile oil comprises the following steps: crushing the Ledum odoratum raw material, passing it through a 20-mesh sieve to obtain Ledum odoratum powder, uniformly mixing the Ledum odoratum powder and an ethanol solution with a volume fraction of 50% at a dosage ratio of 1 g:15 mL, controlling the temperature at 60°C and performing ultrasonic extraction for 18 minutes under the conditions of 210W and 50kHz to obtain an extract, filtering the extract with a 0.45-μm microporous filter membrane to obtain a filtrate, controlling the temperature of the filtrate at 65°C and evaporating the filtrate in a constant temperature water bath to obtain the Cyperus rotundus volatile oil.

The preparation method of the composition having the effect of promoting transdermal absorption specifically comprises the following steps:

The volatile oil of Cyperus rotundus, the volatile oil of Vitex rotundus, the volatile oil of Ledum palustris and propylene glycol were stirred evenly at 25°C, and the temperature was maintained. The kudzu root extract, the lithospermum root extract, the flos centellae extract and the centella asiatica extract were added while stirring for 1 hour to obtain the finished composition.

Example 3

A composition having the effect of promoting transdermal absorption, the composition comprising the following raw materials in parts by weight:

The preparation method of the volatile oil of Cyperus rotundus is as follows: crushing the raw material of Cyperus rotundus, passing it through a 10-mesh sieve to obtain Cyperus rotundus powder, mixing the Cyperus rotundus powder and petroleum ether in a dosage ratio of 1 g:15 mL, controlling the temperature at 47°C, and ultrasonically extracting for 35 minutes under the conditions of 180W and 45kHz to obtain an extract, filtering the extract with a 0.45μm microporous filter membrane to obtain a filtrate, controlling the temperature of the filtrate at 66°C and evaporating it in a constant temperature water bath to obtain the volatile oil of Cyperus rotundus;

The preparation method of the vitex rotundifolia volatile oil comprises the following steps: crushing the vitex rotundifolia raw material, passing through a 20-mesh sieve to obtain vitex rotundifolia powder, uniformly mixing the vitex rotundifolia powder and deionized water at a dosage ratio of 1 g:5.5 mL and heating to boiling, and continuously extracting in an extractor for 6 hours after boiling to obtain an intermediate volatile oil, washing the inner wall of the extractor twice with a small amount of petroleum ether, and uniformly mixing the washing liquid and the intermediate volatile oil to obtain a mixed solution, adding an excess of anhydrous sodium sulfate to the mixed solution, standing for 1 hour and then centrifuging, taking the supernatant and placing it in the air to evaporate the petroleum ether and filtering it with a 0.45 μm microporous filter membrane to obtain the vitex rotundifolia volatile oil;

The preparation method of the Ledum odoratum volatile oil comprises the following steps: crushing the Ledum odoratum raw material, passing it through a 20-mesh sieve to obtain Ledum odoratum powder, uniformly mixing the Ledum odoratum powder and an ethanol solution with a volume fraction of 50% at a dosage ratio of 1 g:17 mL, controlling the temperature at 58° C. and performing ultrasonic extraction for 20 minutes under the conditions of 200 W and 48 kHz to obtain an extract, filtering the extract with a 0.45 μm microporous filter membrane to obtain a filtrate, controlling the temperature of the filtrate at 67° C. and evaporating the filtrate in a constant temperature water bath to obtain the Cyperus rotundus volatile oil.

The preparation method of the composition having the effect of promoting transdermal absorption specifically comprises the following steps:

The volatile oil of Cyperus rotundus, the volatile oil of Vitex rotundus and the volatile oil of Ledum palustris were stirred evenly with n-butanol at 28°C, and the temperature was maintained. The kudzu root extract, the lithospermum root extract, the flos centellae extract and the centella asiatica extract were added while stirring for 1.5 hours to obtain the finished composition.

Comparative Examples 1-3

Compared with Example 3, the difference is that the weight parts of Cyperus rotundus volatile oil, Vitex rotundus volatile oil and Ledum palustris volatile oil in Comparative Examples 1-3 are as shown in Table 1, and the other parameters and operating steps remain unchanged.

Table 1

Comparative Example 4

Compared with Example 3, the difference is that in Comparative Example 4, no volatile oil of Cyperus rotundus, volatile oil of Vitex rotundus and volatile oil of Ledum palustris are added, and the other parameters and operating steps remain unchanged.

Comparative Example 5

Compared with Example 3, the difference is that in Comparative Example 5, no Vitex rotundifolia volatile oil is added, and the other parameters and operating steps remain unchanged.

Comparative Example 6

Compared with Example 3, the difference is that in Comparative Example 6, no Ledum volatile oil is added, and the other parameters and operating steps remain unchanged.

Comparative Examples 7-9

Compared with Example 3, the difference is that the weight proportions of Pueraria root extract, Lithospermum officinale root extract and Centaurea chinensis flower extract in Comparative Examples 7-9 are as shown in Table 2, and the other parameters and operating steps remain unchanged.

Table 2

Comparative Example 10

Compared with Example 3, the difference is that in Comparative Example 10, no Centella asiatica extract is added, and other parameters and operating steps remain unchanged.

Test Example 1

(1) Tested bacteria: Escherichia coli ATCC 8739, Staphylococcus aureus ATCC 6538, Bacillus subtilis ATCC 55614;

(2) Culture medium: Nutrient broth medium: Take 18 g of nutrient broth solid medium, dissolve it in 1000 mL of purified water, sterilize it by high pressure at 121°C for 15 min, and set aside;

(3) Preparation of bacterial suspension: sterilize the test tubes and nutrient broth medium at 121°C for 30 min and set aside. Take 3 sterilized test tubes on a clean bench and add 5 mL of nutrient broth medium to each of them and seal them with silicone plugs. After the medium cools to below 40°C, use an inoculation loop to take appropriate amounts of Escherichia coli, Staphylococcus aureus, and Bacillus subtilis and put them into the test tubes. Mix well and place in a 37°C incubator for 18-24 h. Then use a McFarland turbidimeter to adjust the turbidity of the bacterial suspension to 10 ⁵ CFU/mL and set aside.

(4) Test sample: finished products of the compositions prepared in Examples 1-3 and Comparative Examples 1-9, with a concentration of 2%;

(5) Test method and judgment: Use a pipette to draw 300 μL of bacterial suspension into the solid culture medium and spread it evenly with a spreader. Use sterile tweezers to pick up the Oxford cup and place it on the culture medium with the evenly spread bacterial solution, so that the Oxford cup and the culture medium are in full contact without leaving any gaps. The Oxford cup should be placed in a moderate position. Repeat the experiment three times for each bacterial species. After adding 150 μL of sample solution to each Oxford cup, place the culture dish in an incubator, and place Escherichia coli, Staphylococcus aureus, and Bacillus subtilis in a 37°C incubator for 18-24 hours. Observe the size of the inhibition zone at 18, 21, and 24 hours, and measure the diameter of the inhibition zone with a ruler.

The results are the average of the three measurement data. The inhibition zone diameter greater than 7 mm is judged to have antibacterial effect; the inhibition zone diameter less than or equal to 7 mm is judged to have no antibacterial effect; the larger the inhibition zone diameter, the better the antibacterial effect. The test results are shown in Table 3.

table 3

As can be seen from Table 3, the composition prepared by the present invention has excellent effect of promoting transdermal absorption and antibacterial performance. Specifically, it can be seen from Comparative Examples 7-9 that no matter which one of Pueraria lobata extract, Lithospermum officinale extract and Centella asiatica extract is not added, the antibacterial effect measured is extremely poor, wherein, it is most obvious without adding Pueraria lobata extract; and from Comparative Example 4, it can be seen that if Cyperus rotundus volatile oil, Vitex rotundus volatile oil and Ledum palustris volatile oil are not added, the system lacks the component that promotes transdermal absorption effect, although there is still antibacterial effect in the end, but at the same time, the diameter of its antibacterial circle is not as large as that of Cyperus rotundus volatile oil, Vitex rotundus volatile oil and Ledum palustris volatile oil, that is, the promotion of transdermal absorption at the same time is weak and the antibacterial effect is not so good; and from Comparative Examples 1-3 and Comparative Examples 4-5, it can be seen that no matter which one of Cyperus rotundus volatile oil, Vitex rotundus volatile oil and Ledum palustris volatile oil is not added, the diameter of its antibacterial circle at the same time is not as good as that of Examples 1-3.

Test Example 2

48 volunteers aged 23-50 with healthy skin were randomly selected, regardless of gender, and randomly divided into 4 groups, 12 in each group. A 2cm×2cm square area on the inside of the left and right arms of the volunteers was used as the test area, and the test area was cleaned with clean water. After waiting for 10 minutes, a 50% capsaicin aqueous solution (about 0.01 mL) was used to stimulate the test area for 20 minutes, causing skin allergies, and then the 4 groups of volunteers were evenly smeared with 0.05 mL of the same cream with the composition prepared in Examples 1-3 and Comparative Example 10 (wherein, the amount of the composition added was 4% of the total mass of the cream, and except for the different compositions, the cream matrix was the same), and 60 minutes after applying the cream, the skin erythema value E was measured by a skin colorimeter to reflect the changes in the skin redness and swelling phenomenon, and the average value of each group of data was taken as the final result, thereby feeding back the sensitivity-relieving performance of the test sample, and the results are shown in Table 4.

Table 4

Erythema value after application Example 1 123 Example 2 125 Example 3 129 Comparative Example 4 218

It can be seen from Table 4 that the composition prepared by the present invention has an excellent soothing effect. In detail, by comparing the erythema value E data of Comparative Example 4 and Examples 1-3, it can be seen that the soothing performance of the Centella asiatica extract component is excellent.

In the description of the specification, the description with reference to the terms "one embodiment", "example", "specific example", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

The above contents are merely examples and explanations of the present invention. Those skilled in the art may make various modifications or additions to the specific embodiments described or replace them in a similar manner. As long as they do not deviate from the invention or exceed the scope defined by the claims, they shall all fall within the protection scope of the present invention.

Claims (10)

1. A composition with the effect of promoting transdermal absorption, which is characterized by comprising the following raw materials in parts by weight:

2. the composition with transdermal absorption promoting effect according to claim 1, wherein the preparation method of the nutgrass galingale rhizome volatile oil is as follows: pulverizing rhizoma Cyperi, sieving to obtain rhizoma Cyperi powder, mixing rhizoma Cyperi powder and petroleum ether, ultrasonic extracting at controlled temperature to obtain extractive solution, filtering to obtain filtrate, and volatilizing the filtrate to obtain volatile oil.

3. The composition having an efficacy of promoting transdermal absorption according to claim 2, wherein the ratio of the amount of the nutgrass galingale rhizome powder to the petroleum ether is 1g:10-15mL; the temperature-controlled ultrasonic extraction is specifically carried out for 35-40min under the conditions of 180-200W and 40-45kHz at the temperature of 40-55 ℃.

4. The composition with transdermal absorption promoting effect according to claim 1, wherein the preparation method of the fructus viticis volatile oil is as follows: pulverizing fructus Vitics Simplicifoliae, sieving to obtain fructus Vitics Simplicifoliae Jing Zifen, mixing fructus Vitics Simplicifoliae Jing Zifen with deionized water, heating to boiling, extracting to obtain intermediate volatile oil, washing the extractor with petroleum ether, mixing the washing liquid with the intermediate volatile oil to obtain mixed solution, adding excessive anhydrous sodium sulfate into the mixed solution, standing, centrifuging, collecting supernatant, volatilizing petroleum ether in air, and filtering to obtain fructus Vitics Simplicifoliae volatile oil.

5. The composition of claim 4, wherein the ratio of the tendril Jing Zifen to deionized water is 1g to 5-6mL; the extraction is carried out continuously in an extractor for 6-7h; and the standing and centrifuging is performed after standing for 0.5-1 h.

6. The composition with transdermal absorption promoting effect according to claim 1, wherein the preparation method of the ledum palustre volatile oil is as follows: pulverizing radix Ledebouriellae, sieving to obtain Du Xiangfen, mixing Du Xiangfen with ethanol solution, performing ultrasonic extraction at controlled temperature to obtain extractive solution, filtering to obtain filtrate, and volatilizing at controlled temperature to obtain volatile oil.

7. The composition having an effect of promoting transdermal absorption according to claim 6, wherein the amount ratio of Du Xiangfen to ethanol solution is 1g:15-17mL; the temperature-controlled ultrasonic extraction is specifically carried out for 15-20min under the conditions of 200-210W and 45-50kHz at the temperature of 55-60 ℃.

8. The composition for promoting transdermal absorption according to claim 1, wherein the pueraria extract is obtained by extracting dried root of pueraria lobata of leguminous plant with alcohol as an extraction solvent at a ratio of 1g to 10 ml; the radix Arnebiae extract is prepared by extracting dried radix Arnebiae of Sinkiang of Boraginaceae with ethanol as extraction solvent at the ratio of radix Arnebiae to ethanol of 1 g:10mL; the extract of flos Lonicerae is prepared from herba Polygoni Perfoliati the whole flowers and plants take alcohol as an extraction solvent, extracting according to the dosage ratio of the baijinhua to the alcohol of 1g to 10 mL; the centella asiatica extract is obtained by extracting dried whole herb of centella asiatica with alcohol as an extraction solvent according to the dosage ratio of centella asiatica to alcohol of 1g to 10 mL.

9. The composition having an effect of promoting transdermal absorption according to claim 1, wherein the solvent comprises any one of ethanol, propylene glycol, glycerol, isopropyl alcohol and n-butanol.

10. A method for preparing a composition having an efficacy of promoting transdermal absorption according to any one of claims 1 to 9, characterized in that it comprises the following steps:

stirring rhizoma Cyperi volatile oil, fructus Vitics Simplicifoliae volatile oil, radix aucklandiae volatile oil and solvent at controlled temperature, adding radix Puerariae extract, radix Arnebiae extract, flos Lonicerae extract and herba Centellae extract under stirring, and obtaining the final product.