CN117917952A

CN117917952A - Novel aerosol-generating substrate comprising oregano species

Info

Publication number: CN117917952A
Application number: CN202280048611.6A
Authority: CN
Inventors: D·阿恩特; P·坎帕尼
Original assignee: Philip Morris Products SA
Current assignee: Philip Morris Products SA
Priority date: 2021-07-16
Filing date: 2022-07-14
Publication date: 2024-04-23
Also published as: JP2024529576A; IL309918A; EP4369951A1; CA3224451A1; AU2022311514A1; MX2024000649A; KR20240034217A; WO2023285597A1

Abstract

An aerosol-generating article (1000) (4000 a, 4000 b) (5000) comprises an aerosol-generating substrate (1020) formed from a homogenized oregano material comprising oregano particles, an aerosol-forming agent, and a binder. The aerosol-generating substrate further comprises: at least 50 micrograms ursolic acid per gram of substrate on a dry weight basis; at least 5 micrograms of isothymol per gram of substrate on a dry weight basis; and at least 1.5 micrograms of bailhydroquinone per gram of substrate on a dry weight basis.

Description

Novel aerosol-generating substrate comprising oregano species

The present invention relates to aerosol-generating substrates comprising homogenized plant material formed from oregano particles and aerosol-generating articles incorporating such aerosol-generating substrates. The invention also relates to an aerosol derived from an aerosol-generating substrate comprising oregano particles.

Aerosol-generating articles are known in the art in which an aerosol-generating substrate, such as a tobacco-containing substrate, is heated rather than combusted. Typically in such articles, the aerosol is generated by transferring heat from a heat source to a physically separate aerosol-generating substrate or material that may be positioned in contact with, within, around or downstream of the heat source. During use of the aerosol-generating article, volatile compounds are released from the substrate by heat transfer from the heat source and become entrained in air drawn through the article. As the released compound cools, the compound condenses to form an aerosol.

Some aerosol-generating articles comprise a flavouring agent that is delivered to the consumer during use of the article to provide the consumer with a different sensory experience, for example to enhance the flavour of the aerosol. Flavoring agents may be used to deliver taste (flavor), smell (scent), or both taste and smell to a user inhaling an aerosol. It is known to provide heated aerosol-generating articles comprising a flavour.

It is also known to provide flavoring in conventional combustible flavored cigarettes that are smoked by lighting the end of the cigarette opposite the mouth so that the tobacco rod burns to produce an inhalable smoke. One or more flavoring agents are typically mixed with the tobacco in the tobacco rod to provide additional flavor to the mainstream smoke as the tobacco burns. Such flavoring agents may be provided, for example, as essential oils.

Aerosols from conventional cigarettes comprising a large number of components that interact with the susceptor located in the mouth provide a "full mouth feel" sensation, that is, a relatively full mouth feel. As used herein, "mouthfeel" refers to the physical sensation in the oral cavity caused by food, beverage, or aerosol, and is different from taste. It is a basic sensory attribute that, together with taste and smell, determines the overall flavor of a food or aerosol.

There are difficulties in reproducing the consumer experience provided by conventional combustible cigarettes with aerosol-generating articles in which the aerosol-generating substrate is heated rather than combusted. This is due in part to the lower temperatures reached during heating of such aerosol-generating articles, which results in a different distribution of the volatile compounds released.

It would be desirable to provide a novel aerosol-generating substrate for a heated aerosol-generating article that provides an aerosol with improved flavor and full mouthfeel. It would be particularly desirable if such an aerosol-generating substrate could provide an aerosol with a sensory experience comparable to that provided by conventional combustible cigarettes. It would also be particularly desirable if such an aerosol-generating substrate could provide reduced levels of undesirable aerosol compounds compared to existing aerosol-generating substrates (e.g., those containing only tobacco).

It is also desirable to provide an aerosol-generating substrate that can be easily incorporated into an aerosol-generating article and that can be manufactured using existing high-speed methods and apparatus.

The present disclosure relates to an aerosol-generating article comprising an aerosol-generating substrate formed from homogenized plant material comprising oregano particles, referred to herein as "homogenized oregano material". The homogenized oregano material may further comprise an aerosol former. The homogenized oregano material may also comprise a binder. The aerosol-generating substrate may further comprise at least about 50 micrograms of ursolic acid per gram of substrate on a dry weight basis. The aerosol-generating substrate may further comprise at least about 5 micrograms of isothymol per gram of substrate on a dry weight basis. The aerosol-generating substrate may further comprise at least about 1.5 micrograms of thymoquinone per gram of substrate on a dry weight basis.

According to the present invention there is provided an aerosol-generating article comprising an aerosol-generating substrate formed from a homogenized oregano material comprising oregano particles. According to the invention, the homogenized oregano material comprises: oregano particles, aerosol former, and binder. The aerosol-generating substrate further comprises: at least about 50 micrograms ursolic acid per gram of substrate on a dry weight basis; at least about 5 micrograms of isothymol per gram of substrate on a dry weight basis; and at least about 1.5 micrograms of bailhydroquinone per gram of substrate on a dry weight basis.

Preferably, upon heating an aerosol-generating substrate of an aerosol-generating article according to the invention according to test method a as described below, an aerosol is generated comprising: at least about 1.5 micrograms of ursolic acid per gram of substrate on a dry weight basis; at least about 0.5 micrograms of isothymol per gram of substrate on a dry weight basis; and at least about 0.5 micrograms of bailhydroquinone per gram of substrate on a dry weight basis.

Preferably, upon heating the aerosol-generating substrate according to test method a, the aerosol generated by the aerosol-generating substrate may comprise ursolic acid in an amount of at least about 0.04 micrograms per puff of aerosol. Upon heating the aerosol-generating substrate according to test method a, the aerosol generated by the aerosol-generating substrate can comprise isothymol in an amount of at least about 0.01 micrograms per puff of aerosol. Upon heating the aerosol-generating substrate according to test method a, the aerosol generated by the aerosol-generating substrate may comprise bairimhydroquinone in an amount of at least about 0.01 microgram per puff of aerosol. As generated by the smoking machine, the one puff aerosol has a volume of 55 milliliters.

According to the present invention there is provided an aerosol-generating article comprising an aerosol-generating substrate formed from a homogenized oregano material comprising oregano particles. The aerosol-generating substrate comprises: at least about 50 micrograms ursolic acid per gram of substrate on a dry weight basis; at least about 5 micrograms of isothymol per gram of substrate on a dry weight basis; and at least about 1.5 micrograms of bailhydroquinone per gram of substrate on a dry weight basis.

The present disclosure also relates to an aerosol-generating substrate formed from homogenized plant material comprising oregano particles, referred to herein as "homogenized oregano material". The homogenized oregano material may further comprise an aerosol former. The homogenized plant material may also comprise a binder. The aerosol-generating substrate may comprise at least about 50 micrograms of ursolic acid per gram of substrate on a dry weight basis. The aerosol-generating substrate may comprise at least about 5 micrograms of isothymol per gram of substrate on a dry weight basis. The aerosol-generating substrate may comprise at least about 1.5 micrograms of the bainitic hydroquinone per gram of substrate on a dry weight basis.

According to the present invention there is also provided an aerosol-generating substrate formed from a homogenized oregano material, wherein the homogenized oregano material comprises oregano particles, an aerosol-former, and a binder. The aerosol-generating substrate further comprises: at least 50 micrograms ursolic acid per gram of substrate on a dry weight basis; at least 5 micrograms of isothymol per gram of substrate on a dry weight basis; and at least 1.5 micrograms of bailhydroquinone per gram of substrate on a dry weight basis.

The present disclosure additionally relates to an aerosol generated upon heating an aerosol-generating substrate. The aerosol may comprise ursolic acid in an amount of at least about 0.04 micrograms per puff of aerosol. The aerosol can comprise isothymol in an amount of at least about 0.01 micrograms per puff of aerosol. The aerosol can comprise a quantity of thymoquinone of at least about 0.01 micrograms per puff of aerosol. As generated by the smoking machine, the one puff aerosol has a volume of 55 milliliters.

According to the present invention there is also provided an aerosol generated upon heating an aerosol-generating substrate, the aerosol comprising: ursolic acid in an amount of at least about 0.04 micrograms per puff of aerosol; an amount of isothymol of at least about 0.01 micrograms per puff of aerosol; and an amount of at least about 0.01 micrograms of thymoquinone per puff of aerosol, wherein a puff of aerosol has a volume of 55 milliliters as generated by a smoking machine.

The present invention also provides a method of manufacturing an aerosol-generating substrate comprising: forming a slurry comprising oregano particles, water, an aerosol former, a binder, and optionally tobacco particles; casting or extruding the slurry into the form of a sheet or strand; and drying the sheet or sliver, preferably at a temperature between 80 and 160 degrees celsius. In the case of forming an aerosol-generating substrate sheet, the sheet may optionally be cut into thin strips or gathered to form strips. The sheet may optionally be crimped prior to the gathering step.

Unless otherwise indicated, any reference below to an aerosol-generating substrate and aerosol of the present invention should be considered as applicable to all aspects of the present invention.

As used herein, the term "aerosol-generating article" refers to an article for generating an aerosol, wherein the article comprises an aerosol-generating substrate that is suitable and intended to be heated or combusted in order to release volatile compounds that can form an aerosol. When a user applies a flame to one end of the cigarette and draws air through the other end, the conventional cigarette will be lit. The localized heat provided by the flame and the oxygen in the air drawn through the cigarette causes the ends of the cigarette to be lit and the resulting combustion generates inhalable smoke. In contrast, in a "heated aerosol-generating article", the aerosol is generated by heating the aerosol-generating substrate rather than by burning the aerosol-generating substrate. Heated aerosol-generating articles are known to include, for example, electrically heated aerosol-generating articles and aerosol-generating articles in which an aerosol is generated by heat transfer from a combustible fuel element or heat source to a physically separate aerosol-generating substrate.

Aerosol-generating articles suitable for use in aerosol-generating systems for supplying an aerosol-forming agent to the aerosol-generating article are also known. In such systems, the aerosol-generating substrate in the aerosol-generating article contains significantly less aerosol-forming agent relative to those that carry and provide substantially all of the aerosol-forming agent used in forming an aerosol during operation.

As used herein, the term "aerosol-generating substrate" refers to a substrate capable of generating volatile compounds upon heating that can form an aerosol. The aerosols generated by the aerosol-generating substrate may be visible or invisible to the human eye and may comprise droplets of vapor (e.g., fine particulate matter in the gaseous state, which is typically a liquid or solid at room temperature) as well as gases and condensed vapors.

As used herein, the term "homogenized plant material" encompasses any plant material formed by agglomeration of plant particles. For example, a sheet or web of homogenized plant material for use in an aerosol-generating substrate of the invention may be formed by agglomerating particles of plant material obtained by comminuting, grinding or milling oregano plant material and optionally tobacco material such as tobacco lamina and tobacco stems. The homogenized plant material may be produced by casting, extrusion, papermaking processes, or any other suitable process known in the art.

As used herein, the term "homogenized oregano material" refers to homogenized plant material that comprises oregano particles, optionally in combination with tobacco particles. The term "homogenized tobacco material" refers to homogenized plant material comprising tobacco particles but no oregano particles, which is therefore not according to the invention.

As used herein, the term "oregano particles" encompasses particles derived from the leaves of oregano (Oreganum vulgare). Oreganum vulgare L is a flowering plant of Labiatae, which is native to western and southwest European Asian and Mediterranean regions. Oregano is a culinary herb whose leaves are commonly used to provide a unique flavor to foods.

In contrast, oregano essential oil is a distillate extracted from the leaves of oregano plants. The main flavor compounds in oregano essential oils include isothymol (carvacrol) and thymol.

The present invention provides an aerosol-generating article incorporating an aerosol-generating substrate formed from homogenized plant material comprising oregano particles, referred to herein as homogenized oregano material. The invention also provides an aerosol derived from such an aerosol-generating substrate. The inventors of the present invention have found that by incorporating oregano particles into an aerosol-generating substrate, an aerosol can be advantageously produced that provides a new sensory experience. Such aerosols provide unique flavors and may provide increased levels of fullness.

In addition, the inventors have found that aerosols having improved oregano aroma and flavor can be advantageously produced as compared to aerosols produced by the addition of oregano additives such as oregano oil. Oregano oil (chemical abstracts accession number 8007-11-2) is obtained by steam distillation from the leaves of Oregano plants and has a different flavor composition than Oregano particles, most likely because the distillation process will selectively remove or retain some of the flavor. The isothymol (carvacrol) is the main constituent of oregano oil, accounting for up to 75% of oregano oil.

Further, in certain aerosol-generating substrates provided herein, the oregano particles may be introduced at a sufficient level to provide a desired oregano flavor, while maintaining sufficient tobacco material to provide a desired level of nicotine to the consumer.

Furthermore, it has surprisingly been found that the inclusion of oregano particles in an aerosol-generating substrate provides a significant reduction in certain undesirable aerosol compounds compared to aerosols produced from aerosol-generating substrates comprising 100% tobacco particles without oregano particles. In particular, as shown below, it has surprisingly been found that the inclusion of oregano particles in an aerosol-generating substrate provides a significant reduction in certain phenolic compounds compared to an aerosol generated from an aerosol-generating substrate comprising 100% tobacco particles and no oregano particles. Furthermore, due to the reduction in tobacco particles, it has been found that this reduction is greater than would be expected for a scale calculation.

The presence of oregano in homogenized plant material (e.g., cast leaves) can be positively identified by DNA barcode encoding. Methods for DNA barcoding based on the nuclear gene ITS2, rbcL and matK systems and the plastid gene spacer trnH-psbA are well known in the art and may be used (Chen S, yao H, han J, liu C, song J, et al .(2010)Validation of the ITS2Region as a Novel DNA Barcode for Identifying Medicinal Plant Species.PLoSONE 5(1):e8613;Hollingsworth PM,Graham SW,Little DP(2011)Choosing and Using a Plant DNA Barcode.PLoS ONE 6(5):e19254).

The inventors have re-analyzed and characterized aerosols generated from the aerosol-generating substrate of the invention incorporating oregano particles and mixtures of oregano and tobacco particles and compared these aerosols to those generated from existing aerosol-generating substrates formed from tobacco materials without oregano particles. Based on this, the inventors have been able to identify a set of "signature compounds", which are compounds that are present in aerosols and derived from oregano particles. Thus, detection of these characteristic compounds within a specific weight ratio range within an aerosol can be used to identify aerosols derived from aerosol-generating substrates comprising oregano particles. These characteristic compounds are obviously absent from the aerosols generated from the tobacco material. Furthermore, the ratio of the characteristic compounds and the ratio of the characteristic compounds to each other within the aerosol clearly indicate that oregano plant material is used instead of oregano oil. Similarly, the presence of these characteristic compounds in a particular ratio within an aerosol-generating substrate indicates that the substrate contains oregano particles.

In particular, defined levels of the characteristic compounds within the matrix and aerosol are specific to the oregano particles present within the homogenized oregano material. The level of each characteristic compound depends on the manner in which the oregano particles are processed during the production of the homogenized oregano material. The level also depends on the composition of the homogenized oregano material and, in particular, will be affected by the level of other components within the homogenized oregano material. The level of the characteristic compound within the homogenized oregano material will be different from the level of the same compound within the starting oregano material. It will also differ from the level of characteristic compounds within a material according to the invention that contains oregano particles but not as defined herein.

For characterization of aerosols, the inventors utilized complementary non-targeted differential screening (NTDS) using liquid chromatography coupled to high resolution precision mass spectrometry (LC-HRAM-MS) in parallel with two-dimensional gas chromatography coupled to time-of-flight mass spectrometry (GCxGC-TOFMS).

Non-targeted screening (NTS) is a key method of characterizing the chemical composition of complex matrices by matching unknown detected compound features to a spectral database (suspicious screening analysis [ SSA ]), or if there is no prior knowledge matching, elucidating the structure of the unknown by using information obtained, for example, first order fragmentation (MS/MS) to match computer predicted fragments from the compound database (non-targeted analysis [ NTA ]). It enables the simultaneous measurement of large amounts of small molecules from a sample and the ability to semi-quantify these small molecules using an unbiased method.

If, as described above, the focus is on comparing two or more aerosol samples, any significant differences in chemical composition between samples are evaluated in an unsupervised manner, or if predictors of group correlation between sample groups are available, non-targeted differential screening (NTDS) may be performed. Complementary differential screening methods have been applied using liquid chromatography coupled with high resolution precision mass spectrometry (LC-HRAM-MS) in parallel with two-dimensional gas chromatography coupled to time-of-flight mass spectrometry (GCxGC-TOFMS) in order to ensure comprehensive analytical coverage for identifying the most relevant differences in aerosol composition between aerosols derived from those containing 100 wt% oregano as a particulate plant material and from those containing 100 wt% tobacco as a particulate plant material.

Aerosols are generated and collected using the apparatus and methods described in detail below.

LC-HRAM-MS analysis was performed using Thermo QExactive ^TM high-resolution mass spectrometers in full scan mode and data dependent mode. A total of three different methods are applied to cover a wide range of substances with different ionization properties and compound classes. Samples were analyzed using RP chromatography, using thermal electrospray ionization (HESI) in both positive and negative modes, and Atmospheric Pressure Chemical Ionization (APCI) in positive mode. These methods are described in: arndt, D. ,"In depth characterization of chemical differences between heat-not-burn tobacco products and cigarettes using LC-HRAM-MS-based non-targeted differential screening"(DOI:10.13140/RG.2.2.11752.16643);Wachsmuth,C., et al ,"Comprehensive chemical characterisation of complex matrices through integration of multiple analytical modes and databases for LC-HRAM-MS-based non-targeted screening"(DOI:10.13140/RG.2.2.12701.61927); and "Buchholz, C. et al ,"Increasing confidence for compound identification by fragmentation database and in silico fragmentation comparison with LC-HRAM-MS-based non-targeted screening of complex matrices"(DOI:10.13140/RG.2.2.17944.49927), are both from the 66 th ASMS Mass Spectrometry and related subject conference (ASMS Conference on Mass Spectrometry AND ALLIED Topics, san Diego, USA (2018)). These methods are also described in: arndt, D. Et al ,"A complex matrix characterization approach,applied to cigarette smoke,that integrates multiple analytical methods and compound identification strategies for non-targeted liquid chromatography with high-resolution mass spectrometry"(DOI:10.1002/rcm.8571).

GCxGC-TOFMS analysis was performed using an instrument of type AGILENT GC 6890A or 7890A equipped with an automated liquid injector (type 7683B) and a thermal regulator coupled to a LECO Pegasus 4D ^TM mass spectrometer, with three different methods for non-polar, polar and highly volatile compounds within the aerosol. These methods are described in: ALMSTETTER et al ,"Non-targeted screening using GC×GC-TOFMS for in-depth chemical characterization of aerosol from aheat-not-burn tobacco product"(DOI:10.13140/RG.2.2.36010.31688/1); and ALMSTETTER et al ,"Non-targeted differential screening of complex matrices using GC×GC-TOFMS for comprehensive characterization of the chemical composition and determination of significant differences"(DOI:10.13140/RG.2.2.32692.55680), are from the 66 th and 64 th ASMS mass spectra and related subject matter conference (ASMS Conferences on Mass Spectrometry AND ALLIED Topics, san Diego, USA), respectively.

The results of the analytical methods provide information about the primary compounds that caused the aerosol differences generated by these articles. Non-targeted differential screening using analytical platforms LC-HRAM-MS and GCxGC-TOFMS focused on compounds present in greater amounts in aerosols of samples of aerosol-generating substrates according to the invention comprising 100% oregano particles relative to comparative samples of aerosol-generating substrates comprising 100% tobacco particles. The NTDS method is described in the above-mentioned literature.

Based on this information, the inventors were able to identify specific compounds within the aerosol that can be considered to be "signature compounds" derived from oregano particles in the matrix. Characteristic compounds derived from oregano include, but are not limited to: ursolic acid (3β -hydroxy urso-12-en-28-oic acid) of formula: c ₃₀H₄₈O₃, chemical abstracts accession number 77-52-1); isothymol, also known as carvacrol or carvacrol (2-methyl-5- (prop-2-yl) phenol), has the formula: c ₁₀H₁₄ O, chemical abstracts accession number 499-75-2); and baili hydroquinone (2-methyl-5-prop-2-ylbenzene-1, 4-diol), of the formula: c ₁₀H₁₄O₂, chemical abstracts accession number 2217-60-9).

For the purposes of the present invention, samples of aerosol-generating substrates may be subjected to targeted screening to identify the presence and amount of each characteristic compound in the substrate. This targeted screening method is described below. As described, the characteristic compounds may be detected and measured in aerosol-generating substrates and aerosols derived from aerosol-generating substrates.

As defined above, the aerosol-generating article of the invention comprises an aerosol-generating substrate formed from homogenized plant material comprising oregano particles. The aerosol-generating substrate comprises a proportion of the "characteristic compounds" of oregano as described above, as a result of comprising oregano particles. In particular, the aerosol-generating substrate preferably comprises at least 50 micrograms of ursolic acid per gram of substrate, at least 5 micrograms of isothymol per gram of substrate, and at least 1.5 micrograms of thymol per gram of substrate on a dry weight basis.

By defining the aerosol-generating substrate relative to the desired level of the characteristic compound, consistency between products can be ensured despite potential differences in the level of the characteristic compound in the raw material. This advantageously enables a more efficient control of the quality of the product.

Preferably, the aerosol-generating substrate comprises at least about 200 micrograms of ursolic acid per gram of substrate on a dry weight basis, more preferably at least about 500 micrograms of ursolic acid per gram of substrate. Alternatively or additionally, the aerosol-generating substrate preferably comprises no more than about 6500 micrograms of ursolic acid per gram of substrate, more preferably no more than about 4000 micrograms of ursolic acid per gram of substrate, more preferably no more than about 2500 micrograms of ursolic acid per gram of substrate on a dry weight basis.

For example, the aerosol-generating substrate may comprise about 50 micrograms to about 6500 micrograms of ursolic acid per gram of substrate, or about 200 micrograms to about 4000 micrograms of ursolic acid per gram of substrate, or about 500 micrograms to about 2500 micrograms of ursolic acid per gram of substrate on a dry weight basis.

In certain particularly preferred embodiments, the aerosol-generating substrate may comprise from about 1000 micrograms to about 2000 micrograms of ursolic acid per gram of aerosol-generating substrate, more preferably from about 1200 micrograms to about 1800 micrograms of ursolic acid per gram of aerosol-generating substrate. For example, for a preferred embodiment of the invention, the level of ursolic acid may be within these ranges, wherein the aerosol generating substrate comprises from 15 wt% to 20 wt% of oregano particles on a dry weight basis.

Preferably, the aerosol-generating substrate comprises at least about 50 micrograms of isothymol per gram of substrate, more preferably at least about 100 micrograms of isothymol per gram of substrate, on a dry weight basis. Alternatively or additionally, the aerosol-generating substrate preferably comprises not more than about 600 micrograms of isothymol per gram of substrate, more preferably not more than about 400 micrograms of isothymol per gram of substrate, more preferably not more than about 250 micrograms of isothymol per gram of substrate on a dry weight basis.

For example, the aerosol-generating substrate may comprise from about 5 micrograms to about 600 micrograms of isothymol per gram of substrate, or from about 50 micrograms to about 400 micrograms of isothymol per gram of substrate, or from about 100 micrograms to about 250 micrograms of isothymol per gram of substrate, on a dry weight basis.

In certain particularly preferred embodiments, the aerosol-generating substrate may comprise from about 100 micrograms to about 200 micrograms of isothymol per gram of aerosol-generating substrate, more preferably from about 120 micrograms to about 160 micrograms of isothymol per gram of aerosol-generating substrate. For example, for a preferred embodiment of the invention, the level of isothymol may be within these ranges, wherein the aerosol-generating substrate comprises from 15wt% to 20wt% oregano particles on a dry weight basis.

Preferably, the aerosol-generating substrate comprises at least about 10 micrograms of thymoquinone per gram of substrate on a dry weight basis, more preferably at least about 25 micrograms of thymoquinone per gram of substrate. Alternatively or additionally, the aerosol-generating substrate preferably comprises no more than about 200 micrograms of thymoquinone per gram of substrate, more preferably no more than about 150 micrograms of thymoquinone per gram of substrate, more preferably no more than about 100 micrograms of thymohydroquinone per gram of substrate on a dry weight basis.

For example, the aerosol-generating substrate may comprise from about 1.5 micrograms to about 200 micrograms of the thymoquinone per gram of the substrate, or from about 10 micrograms to about 150 micrograms of the thymoquinone per gram of the substrate, or from about 25 micrograms to about 100 micrograms of the thymohydroquinone per gram of the substrate, on a dry weight basis.

In certain particularly preferred embodiments, the aerosol-generating substrate may comprise from about 20 micrograms to about 60 micrograms of thymoquinone per gram of aerosol-generating substrate, more preferably from about 30 micrograms to about 50 micrograms of thymohydroquinone per gram of aerosol-generating substrate. For example, for a preferred embodiment of the invention, the level of the bai hydroquinone may be within these ranges, wherein the aerosol-generating substrate comprises from 15 wt% to 20 wt% of oregano particles on a dry weight basis.

Preferably, the ratio of the characteristic compound in the aerosol-generating substrate is such that the amount of ursolic acid per gram of substrate is at least 8 times the amount of isothymol per gram of substrate, more preferably at least 10 times the amount of isothymol per gram of substrate.

Preferably, the ratio of the characteristic compound in the aerosol-generating substrate is such that the amount of ursolic acid per gram of substrate is at least 25 times the amount of thymoquinone per gram of substrate, more preferably at least 30 times the amount of thymohydroquinone per gram of substrate.

These ratios of ursolic acid to isothymol and thymol are characteristic of inclusion of oregano particles in an aerosol generating substrate.

As defined above, the present invention also provides an aerosol-generating article comprising an aerosol-generating substrate formed from homogenized plant material comprising particles of oregano, wherein an aerosol comprising a "feature compound" of oregano is generated upon heating the aerosol-generating substrate.

For the purposes of the present invention, the aerosol-generating substrate is heated according to "test method a". In test method a, an aerosol-generating article incorporating an aerosol-generating substrate is heated in a tobacco heating system 2.2 holder (THS 2.2 holder) under HEALTH CANADA machine smoking regimen. For the purpose of performing test method a, an aerosol-generating substrate was provided in an aerosol-generating article compatible with the THS2.2 holder.

The tobacco heating system 2.2 holder (THS 2.2 holder) corresponds to a commercially available IQOS device (Philip Morris Products SA (switzerland)) as described in Smith et al, 2016, regul. Toxicol. Pharmacol.81 (S2) S82-S92. Aerosol-generating articles for use in conjunction with IQOS devices are also commercially available.

HEALTH CANADA smoking regime is a well-defined and accepted smoking regime as defined in publication HEALTH CANADA 2000-Tobacco Products Information Regulations SOR/2000-273, schedule 2 (health Canadian 2000-tobacco product information Act SOR/2000-273, plan 2) of Ministry of Justice Canada. The test method is described in ISO/TR 19478-1:2014. In the HEALTH CANADA smoking test, 12 puffs of aerosol were collected from a sample aerosol-generating substrate, the puff volume was 55 mm, the duration of the puff was 2 seconds, the puff interval was 30 seconds, and if ventilation was present, all ventilation was blocked.

Thus, in the context of the present invention, the expression "when heating an aerosol-generating substrate according to test method a" means when heating an aerosol-generating substrate in a THS2.2 holder under the HEALTH CANADA machine smoking regime defined in plan 2 under the health canada 2000-tobacco product information regulations SOR/2000-273, as published Ministry of Justice Canada, the test method being described in ISO/TR 19478-1:2014.

For analytical purposes, depending on the analytical method to be used, the aerosol generated from the heated aerosol-generating substrate is captured using a suitable device. In a suitable method of producing samples for LC-HRAM-MS analysis, a conditioned 44mm Cambridge glass fiber filter pad (according to ISO 3308) and a filter paper clip (according to ISO 4387 and ISO 3308) are used to trap the particulate phase. The remaining gas phase was collected downstream from the filter pad using two consecutive micro dust meters (20 mL), each containing methanol and an Internal Standard (ISTD) solution (10 mL), maintained at-60 degrees celsius using a dry ice-isopropanol mixture. The trapped particulate and gas phases were then recombined and extracted using methanol from a miniature dust tester by shaking the sample, vortexing for 5 minutes and centrifuging (4500 g,5 minutes, 10 ℃). The resulting extract was diluted with methanol and mixed in Eppendorf ThermoMixer (5 ℃,2000 rpm). Test samples from the extracts were analyzed by LC-HRAM-MS in a combined full scan mode and data dependent fragmentation mode to identify the signature compounds. For the purposes of the present invention, LC-HRAM-MS analysis is suitable for the identification and quantification of ursolic acid.

Samples for GCxGC-TOFMS analysis can be produced in a similar manner, but for GCxGC-TOFMS analysis, different solvents are suitable for extracting and analyzing polar, non-polar and volatile compounds separated from the whole aerosol.

For both non-polar and polar compounds, a conditioned 44mm Cambridge glass fiber filter pad (according to ISO 3308) and a filter paper clip (according to ISO 4387 and ISO 3308) were used, and then the entire aerosol was collected using two miniature dust meters connected and sealed in series. Each micro dust meter (20 mL) contained 10mL of methylene chloride/methanol (80:20 v/v) containing an Internal Standard (ISTD) and a Retention Index Marker (RIM) compound. The micro dust meter was kept at-80 ℃ using a dry ice-isopropanol mixture. For analysis of the non-polar compounds, the contents of a miniature dust tester were used to extract the entire aerosol particle phase from the glass fiber filter pad. Water was added to an aliquot of the resulting extract (10 mL) and the sample was shaken and centrifuged as described above. The dichloromethane layer was separated, dried over sodium sulfate and analyzed by GCxGC-TOFMS in full scan mode. For analysis of polar compounds, the remaining aqueous layer from the above-described non-polar sample preparation was used. The ISTD and RIM compounds were added to the aqueous layer and then analyzed directly in full scan mode by GCxGC-TOFMS.

For volatile compounds, the full aerosol was collected using two serially connected and sealed micro dust meters (20 mL), each filled with 10mL of N, N-Dimethylformamide (DMF) containing ISTD and RIM compounds. The micro dust tester was maintained at-50 ℃ to-60 ℃ using a dry ice-isopropanol mixture. After collection, the contents of the two miniature dust-measuring devices were combined and analyzed in full scan mode by GCxGC-TOFMS.

For the purposes of the present invention, GCxGC-TOFMS analysis is suitable for the identification and quantification of isothymol and thymol hydroquinone.

The aerosol generated upon heating the aerosol-generating substrate of the invention according to test method a is preferably characterized by the amounts and ratios of the characterizing compounds ursolic acid, isothymol and thymol hydroquinone as defined above.

Preferably, in an aerosol-generating article comprising an aerosol-generating substrate as described above, upon heating the aerosol-generating substrate according to test method a, an aerosol is generated comprising: at least 1.5 micrograms ursolic acid per gram of substrate on a dry weight basis; at least 0.5 micrograms of isothymol per gram of substrate on a dry weight basis; and at least 0.5 micrograms of bailhydroquinone per gram of substrate on a dry weight basis.

The range defines the amount of each characteristic compound in the aerosol generated per gram of aerosol-generating substrate (also referred to herein as "substrate"). This is equal to the total amount of the characteristic compounds measured in the aerosol collected during test method a divided by the dry weight of the aerosol-generating substrate prior to heating.

Upon heating the aerosol-generating substrate according to test method a, preferably the aerosol generated preferably comprises at least about 10 micrograms of ursolic acid per gram of substrate on a dry weight basis. More preferably, the aerosol generated from the aerosol-generating substrate according to the invention comprises at least about 25 micrograms of ursolic acid per gram of substrate on a dry weight basis.

Alternatively or additionally, the aerosol generated from the aerosol-generating substrate preferably comprises up to about 200 micrograms of ursolic acid per gram of substrate on a dry weight basis. More preferably, the aerosol generated from the aerosol-generating substrate comprises up to about 150 micrograms of ursolic acid per gram of substrate on a dry weight basis. Even more preferably, the aerosol generated from the aerosol-generating substrate comprises up to about 100 micrograms of ursolic acid per gram of substrate on a dry weight basis.

Upon heating the aerosol-generating substrate according to test method a, the aerosol generated preferably comprises at least about 5 micrograms of isothymol per gram of substrate on a dry weight basis. More preferably, the aerosol generated from the aerosol-generating substrate according to the invention comprises at least about 10 micrograms of isothymol per gram of substrate on a dry weight basis.

Alternatively or additionally, the aerosol generated from the aerosol-generating substrate preferably comprises at most about 50 micrograms of isothymol per gram of substrate on a dry weight basis. More preferably, the aerosol generated from the aerosol-generating substrate comprises up to about 35 micrograms of isothymol per gram of substrate on a dry weight basis. Even more preferably, the aerosol generated from the aerosol-generating substrate comprises at most about 25 micrograms of isothymol per gram of substrate on a dry weight basis.

Upon heating the aerosol-generating substrate according to test method a, the aerosol generated preferably comprises at least about 5 micrograms of thymoquinone per gram of substrate on a dry weight basis. More preferably, the aerosol generated from the aerosol-generating substrate according to the invention comprises at least about 10 micrograms of thymoquinone per gram of substrate on a dry weight basis.

Alternatively or additionally, the aerosol generated from the aerosol-generating substrate preferably comprises up to about 50 micrograms of thymoquinone per gram of substrate on a dry weight basis. More preferably, the aerosol generated from the aerosol-generating substrate comprises up to about 35 micrograms of thymoquinone per gram of substrate on a dry weight basis. Even more preferably, the aerosol generated from the aerosol-generating substrate comprises up to about 25 micrograms of thymoquinone per gram of substrate on a dry weight basis.

Preferably, the aerosol generated from the aerosol-generating substrate according to the invention during test method a further comprises at least about 0.1 micrograms of nicotine per gram of substrate, more preferably at least about 1 microgram of nicotine per gram of substrate, more preferably at least about 2 micrograms of nicotine per gram of substrate on a dry weight basis. Preferably, the aerosol comprises up to about 10 micrograms of nicotine per gram of substrate, more preferably up to about 7.5 micrograms of nicotine per gram of substrate, more preferably up to about 4 micrograms of nicotine per gram of substrate on a dry weight basis. For example, the aerosol may comprise about 0.1 micrograms to about 10 micrograms of nicotine per gram of substrate, or about 1 micrograms to about 7.5 micrograms of nicotine per gram of substrate, or about 2 micrograms to about 4 micrograms of nicotine per gram of substrate on a dry weight basis. In some embodiments of the invention, the aerosol may contain zero micrograms of nicotine.

Various methods known in the art can be applied to measure the amount of nicotine in an aerosol.

Carbon monoxide may also be present in the aerosol generated by the aerosol-generating substrate according to the invention during test method a and may be measured and used for further characterization of the aerosol. Nitrogen oxides such as nitric oxide and nitrogen dioxide may also be present in the aerosol and may be measured and used to further characterize the aerosol.

According to the invention, the aerosol generated from the aerosol-generating substrate during test method a preferably has an amount of ursolic acid per gram of substrate of at least 2 times the amount of isothymol per gram of substrate. Thus, the ratio of ursolic acid to isothymol is at least 2:1. More preferably, the amount of ursolic acid in the aerosol generated from the aerosol generating substrate during test method a is at least 3 times the amount of isothymol per gram of substrate such that the ratio of ursolic acid to isothymol is at least 3:1.

According to the invention, the aerosol generated from the aerosol-generating substrate during test method a preferably has an amount of ursolic acid per gram of substrate of at least 2 times the amount of bairimhydroquinone per gram of substrate. Thus, the ratio of ursolic acid to bai hydroquinone is at least 2:1. More preferably, the amount of ursolic acid in the aerosol generated from the aerosol generating substrate during test method a is at least 3 times the amount of thymoquinone per gram of substrate such that the ratio of ursolic acid to thymohydroquinone is at least 3:1.

The defined ratio of ursolic acid to isothymol and thymol hydroquinone characterizes aerosols derived from oregano particles. In contrast, in aerosols produced from oregano oil, the ratio of ursolic acid to isothymol and thymol hydroquinone will be significantly different.

The aerosol generated from the aerosol-generating substrate according to the invention during test method a may further comprise at least about 5mg of aerosol-former per gram of aerosol-generating substrate, or at least about 10mg of aerosol per gram of substrate, or at least about 15mg of aerosol-former per gram of substrate. Alternatively or additionally, the aerosol may comprise up to about 30 milligrams of aerosol former per gram of matrix, or up to about 25 milligrams of aerosol former per gram of matrix, or up to about 20 milligrams of aerosol former per gram of matrix. For example, the aerosol may comprise from about 5mg to about 30 mg of aerosol former per gram of matrix, or from about 10mg to about 25 mg of aerosol former per gram of matrix, or from about 15mg to about 20 mg of aerosol former per gram of matrix. In alternative embodiments, the aerosol may comprise less than 5 milligrams of aerosol former per gram of substrate. This may be suitable, for example, if the aerosol-forming agent is provided separately within the aerosol-generating article or the aerosol-generating device.

Suitable aerosol formers for use in the present invention are described below.

Various methods known in the art may be applied to measure the amount of aerosol former in an aerosol.

As described above, the presence of the characteristic compounds in the aerosol in defined amounts and ratios indicates that the oregano particles are contained in the homogenized plant material forming the aerosol generating substrate.

Preferably, the aerosol-generating substrate according to the invention comprises a homogenized oregano material comprising at least about 0.5% by weight of oregano particles on a dry weight basis. Preferably, the homogenized oregano material comprises at least about 0.75 weight percent oregano particles, more preferably at least about 1.5 weight percent oregano particles, more preferably at least about 2.5 weight percent oregano particles, more preferably at least about 3 weight percent oregano particles, more preferably at least about 4 weight percent oregano particles, more preferably at least about 5 weight percent oregano particles, more preferably at least about 6 weight percent oregano particles, more preferably at least about 7 weight percent oregano particles, more preferably at least about 8 weight percent oregano particles, more preferably at least about 9 weight percent oregano particles, more preferably at least about 10 weight percent oregano particles, on a dry weight basis.

In certain embodiments of the invention, the plant particles forming the homogenized oregano material may comprise at least 99.5 wt% oregano particles or at least 95 wt% oregano particles or at least 90 wt% oregano particles, based on the dry weight of the plant particles. In such embodiments, the aerosol-generating substrate thus comprises oregano particles, while being substantially free of other plant particles. For example, the plant particles forming the homogenized oregano material may comprise about 100 weight percent oregano particles.

In an alternative embodiment of the invention, as described below, the homogenized oregano material may comprise a combination of oregano particles and tobacco particles.

In the following description of the invention, the term "particulate plant material" is used to generically refer to plant material particles used to form homogenized plant material. The particulate plant material may consist essentially of oregano particles, or may be a mixture of oregano particles and tobacco particles.

The homogenized oregano material may comprise up to about 100 weight percent oregano particles on a dry weight basis. Preferably, the homogenized oregano material comprises at most about 90 wt% oregano particles, more preferably at most about 80 wt% oregano particles, more preferably at most about 70 wt% oregano particles, more preferably at most about 60 wt% oregano particles, more preferably at most about 50 wt% oregano particles on a dry weight basis.

For example, the homogenized oregano material may comprise from about 0.5 wt% to about 100 wt% oregano particles, or from about 2.5 wt% to about 90 wt% oregano particles, or from about 10 wt% to about 80 wt% oregano particles, or from about 15 wt% to about 70 wt% oregano particles, or from about 20 wt% to about 60 wt% oregano particles, or from about 30 wt% to about 50 wt% oregano particles, on a dry weight basis.

In certain particularly preferred embodiments of the present invention, the homogenized oregano material comprises from about 15 weight percent to about 20 weight percent oregano particles on a dry weight basis.

As described above, the inventors have identified a number of "signature compounds" which are signature compounds of oregano plants and thus indicate the inclusion of oregano plant particles within an aerosol-generating substrate.

The amount of the characteristic compound present in the pure oregano particles is expected to be different from the amount present in the aerosol-generating substrate. The presence of other ingredients such as aerosol formers, which involve the process of preparing a matrix that is hydrated in a slurry or suspension and dried at an elevated temperature, will differentially vary the amount of each of the characteristic compounds. The integrity of the oregano particles and the stability of the compounds under temperature and handling during manufacture will also affect the final amount of compounds present in the matrix. It is therefore envisaged that the ratio of the characteristic compounds relative to each other will be different after the oregano particles are introduced into the matrix in various physical forms such as sheets, strips and particles.

The presence of oregano in the aerosol-generating substrate and the proportion of oregano provided in the aerosol-generating substrate may be determined by measuring the amount of the characteristic compound in the substrate and comparing it to the corresponding amount of the characteristic compound in the pure oregano material. The presence and amount of the characteristic compounds may be carried out using any suitable technique known to those skilled in the art.

In a suitable technique, a sample of 250 mg of aerosol-generating substrate is mixed with 5ml of methanol and extracted by shaking, vortexing for 5 minutes and centrifugation (4500 g,5 minutes, 10 degrees celsius). An aliquot of the extract (300 microliters) was transferred to a silanized chromatography vial and diluted with methanol (600 microliters) and Internal Standard (ISTD) solution (100 microliters). The vials were closed and mixed for 5 minutes using Eppendorf ThermoMixer (5 degrees celsius; 2000 rpm). Test samples from the resulting extracts were analyzed by LC-HRAM-MS in a combined full scan mode and data dependent fragmentation mode to identify characteristic compounds.

In some embodiments, the homogenized oregano material further comprises up to about 75 weight percent tobacco particles on a dry weight basis.

For example, the homogenized oregano material preferably comprises about 10 wt.% to about 75 wt.% tobacco particles, more preferably about 15 wt.% to about 70 wt.% tobacco particles, more preferably about 20 wt.% to about 65 wt.% tobacco particles, more preferably about 25 wt.% to about 60 wt.% tobacco particles, more preferably about 30 wt.% to about 70 wt.% tobacco particles, on a dry weight basis.

In some preferred embodiments, the homogenized oregano material comprises from about 5 weight percent to about 20 weight percent oregano particles and from about 55 weight percent to about 70 weight percent tobacco particles on a dry weight basis.

The weight ratio of the oregano particles and tobacco particles in the particulate plant material forming the homogenized oregano material may vary depending on the desired flavor profile and the composition of the aerosol. Preferably, the homogenized oregano material comprises a weight ratio of oregano particles to tobacco particles of no more than 1:4. This means that the oregano particles comprise no more than 20% of the total particulate plant material. More preferably, the homogenized oregano material comprises a weight ratio of oregano particles to tobacco particles of no more than 1:5, more preferably no more than 1:6.

For example, in a first preferred embodiment, the weight ratio of oregano particles to tobacco particles is 1:4. The 1:4 ratio corresponds to a granular plant material consisting of about 20% by weight of oregano particles and about 80% by weight of tobacco particles. For homogenized oregano material formed with about 75 weight percent particulate plant material, this corresponds to about 15 weight percent oregano particles and about 60 weight percent tobacco particles in the homogenized oregano material on a dry weight basis.

In another embodiment, the homogenized oregano material comprises oregano particles and tobacco particles in a weight ratio of 1:9. In yet another embodiment, the homogenized oregano material comprises oregano particles to tobacco particles in a weight ratio of 1:30.

With reference to the present invention, the term "tobacco particles" describes particles of any plant member of the genus nicotiana. The term "tobacco particles" includes ground or crushed tobacco lamina, ground or crushed tobacco leaf stem, tobacco dust, tobacco fines and other particulate tobacco by-products formed during the handling, manipulation and transportation of tobacco. In a preferred embodiment, the tobacco particles are substantially entirely derived from tobacco lamina. In contrast, the isolated nicotine and nicotine salts are tobacco-derived compounds, but are not considered tobacco particles for the purposes of the present invention and are not included in the percentage of particulate plant material.

The tobacco particles may be prepared from one or more tobacco plants. Any type of tobacco may be used in the blend. Examples of types of tobacco that may be used include, but are not limited to, sun-cured tobacco, flue-cured tobacco, burley tobacco, maryland tobacco (Maryland tobacco), oriental tobacco (Oriental tobacco), virginia tobacco (Virginia tobacco), and other specialty tobaccos.

Flue-cured tobacco is a method of curing tobacco, particularly with virginia tobacco. During the baking process, heated air is circulated through the densely packed tobacco. During the first stage, the tobacco leaves yellow and wilt. During the second stage, the leaves' leaves are completely dried. In the third stage, the peduncles are completely dried.

Burley tobacco plays an important role in many tobacco blends. Burley tobacco has a distinctive flavor and aroma, and also has the ability to absorb large amounts of the charge (casing).

Oriental tobacco is a tobacco having lamina and high aromatic quality. However, the flavor of Oriental tobacco is milder than that of burley tobacco, for example. Thus, a relatively small proportion of Oriental tobacco is typically used in tobacco blends.

Kasturi, madura and Jatim are all useful subtypes of sun-cured tobacco. Preferably Kasturi tobacco and flue-cured tobacco can be used in the mixture to produce tobacco particles. Thus, the tobacco particles in the particulate plant material may comprise a mixture of Kasturi tobacco and flue-cured tobacco.

The tobacco particles can have a nicotine content of at least about 2.5% by weight on a dry weight basis. More preferably, the tobacco particles can have a nicotine content of at least about 3% by weight, even more preferably at least about 3.2% by weight, even more preferably at least about 3.5% by weight, most preferably at least about 4% by weight on a dry weight basis. When the aerosol-generating substrate contains a combination of tobacco particles and oregano particles, it is preferred to maintain a similar level of nicotine with tobacco having a higher nicotine content as a typical aerosol-generating substrate without oregano particles, as otherwise the total amount of nicotine would be reduced by replacing the tobacco particles with oregano particles.

Alternatively, the tobacco particles can have a nicotine content of less than about 2.5% by weight on a dry weight basis. For example, the tobacco particles can have a nicotine content of less than about 2 wt%, or less than or 1.5 wt%, or less than about 1wt%, on a dry weight basis. In some embodiments, the tobacco particles may have a substantially zero nicotine level.

The aerosol-generating substrate of such an embodiment and the aerosol generated from the aerosol-generating substrate of such an embodiment comprise a proportion of tobacco "signature compounds" as a result of the inclusion of tobacco particles. Characteristic compounds produced from tobacco include, but are not limited to, neonicotinoid, cotinine, and damascenone.

Nicotine may optionally be introduced into the aerosol-generating substrate, but for the purposes of the present invention this will be considered a non-tobacco material. The nicotine may comprise one or more nicotine salts selected from the list of: nicotine lactate, nicotine citrate, nicotine pyruvate, nicotine bitartrate, nicotine benzoate, nicotine pectate, nicotine alginate and nicotine salicylate. In addition to tobacco having a low nicotine content, nicotine may also be introduced, or nicotine may be introduced into an aerosol-generating substrate having a reduced or zero tobacco content.

In certain embodiments of the invention, the aerosol-generating substrate comprises a homogenized oregano material formed from a particulate plant material consisting solely of oregano particles, wherein nicotine, such as a nicotine salt, is introduced into the aerosol-generating substrate.

Preferably, the aerosol-generating substrate comprises at least about 0.1mg nicotine per gram of substrate on a dry weight basis. More preferably, the aerosol-generating substrate comprises at least about 0.5mg nicotine per gram of substrate, more preferably at least about 1mg nicotine per gram of substrate, more preferably at least about 1.5mg nicotine per gram of substrate, more preferably at least about 2mg nicotine per gram of substrate, more preferably at least about 3mg nicotine per gram of substrate, more preferably at least about 4mg nicotine per gram of substrate, more preferably at least about 5mg nicotine per gram of substrate on a dry weight basis.

Preferably, the aerosol-generating substrate comprises at most about 50mg of nicotine per gram of substrate on a dry weight basis. More preferably, the aerosol-generating substrate comprises at most about 45mg of nicotine per gram of substrate, more preferably at most about 40mg of nicotine per gram of substrate, more preferably at most about 35mg of nicotine per gram of substrate, more preferably at most about 30mg of nicotine per gram of substrate, more preferably at most about 25mg of nicotine per gram of substrate, more preferably at most about 20mg of nicotine per gram of substrate on a dry weight basis.

For example, the aerosol-generating substrate may comprise about 0.1mg to about 50mg of nicotine per gram of substrate, or about 0.5mg to about 45mg of nicotine per gram of substrate, or about 1mg to about 40mg of nicotine per gram of substrate, or about 2mg to about 35mg of nicotine per gram of substrate, or about 5mg to about 30mg of nicotine per gram of substrate, or about 10mg to about 25mg of nicotine per gram of substrate, or about 15mg to about 20mg of nicotine per gram of substrate on a dry weight basis. In certain preferred embodiments of the invention, the aerosol-generating substrate comprises from about 1mg to about 20mg of nicotine per gram of substrate on a dry weight basis.

The defined range of nicotine content of the aerosol-generating substrate includes all forms of nicotine that may be present in the aerosol-generating substrate, including nicotine inherently present in the tobacco material and nicotine that has optionally been added separately to the aerosol-generating substrate, e.g. in the form of a nicotine salt.

In some embodiments, the aerosol-generating substrate comprises substantially zero nicotine.

For example, the particulate plant material may comprise from about 45% to about 60% by weight tobacco particles, more preferably from about 50% to about 65% by weight tobacco particles, on a dry weight basis.

In addition to the oregano particles or the combination of oregano particles and tobacco particles ("particulate plant material"), the homogenized oregano material may also contain a proportion of other plant flavor particles.

For the purposes of the present invention, the term "other plant flavour particles" refers to particles of plant material other than oregano, non-tobacco and non-cannabis, which are capable of generating one or more flavourings upon heating. The term should be regarded as excluding particles of inert plant material such as cellulose, which do not contribute to the sensory output of the aerosol-generating substrate. The particles may be from ground or crushed leaves, fruits, stems, stalks, roots, seeds, shoots or bark of other plants. Suitable plant flavour particles for inclusion in an aerosol-generating substrate according to the invention will be known to the skilled person and include, but are not limited to, clove particles, tea particles, bamboo particles and combinations thereof.

The composition of the homogenized oregano material may be advantageously adjusted by blending the desired amounts and types of different plant particles. This enables the aerosol-generating substrate to be formed from a single homogenised oregano material without the need to combine or mix different blends if required, as is the case in the production of conventional cut filler materials. Thus, the production of aerosol-generating substrates can potentially be simplified.

The particulate plant material used in the aerosol-generating substrate of the present invention may be adapted to provide a desired particle size distribution. The particle size distribution is herein expressed in terms of D-values, wherein D-values refer to the number percentage of particles having a diameter less than or equal to a given D-value. For example, in a D95 particle size distribution, 95% by number of the particles have a diameter less than or equal to a given D95 value, and 5% by number of the particles have a diameter greater than the given D95 value. Similarly, in a D5 particle size distribution, 5% of the number of particles have a diameter less than or equal to the D5 value, and 95% of the number of particles have a diameter greater than the given D5 value. The D5 and D95 values combine to thereby provide an indication of the particle size distribution of the particulate plant material.

The particulate plant material may have a D95 value of greater than or equal to 50 microns to a D95 value of less than or equal to 400 microns. This means that the particulate plant material may have a distribution represented by any D95 value within the given range, i.e. D95 may be equal to 50 microns, or D95 may be equal to 55 microns, etc., until D95 may be equal to 400 microns. By providing a D95 value within this range, inclusion of relatively large plant particles in the homogenized oregano material is avoided. This is desirable because generating aerosols from such large plant particles can be relatively inefficient. Furthermore, the inclusion of large plant particles in the homogenized oregano material may adversely affect the consistency of the material.

Preferably, the particulate plant material may have a D95 value of greater than or equal to about 50 microns to a D95 value of less than or equal to about 350 microns, more preferably a D95 value of greater than or equal to about 75 microns to a D95 value of less than or equal to about 300 microns. Both the particulate oregano material and the particulate tobacco material may have a D95 value of greater than or equal to about 50 microns to a D95 value of less than or equal to about 400 microns, preferably a D95 value of greater than or equal to 75 microns to a D95 value of less than or equal to about 350 microns, more preferably a D95 value of greater than or equal to about 100 microns to a D95 value of less than or equal to about 300 microns.

Preferably, the particulate plant material may have a D5 value of greater than or equal to about 10 microns to a D5 value of less than or equal to about 50 microns, more preferably a D5 value of greater than or equal to about 20 microns to a D5 value of less than or equal to about 40 microns. By providing a D5 value within this range, the inclusion of very small dust particles in the homogenized oregano material may be avoided, which may be desirable from a manufacturing standpoint.

In some embodiments, the granular plant material comprising oregano particles may be purposefully milled to form particles having a desired particle size distribution. The use of purposefully milled plant material advantageously improves the uniformity of the particulate plant material and the consistency of the homogenized oregano material.

100% Of the particulate plant material may have a diameter of less than or equal to about 300 microns, more preferably less than or equal to about 275 microns. 100% of the particulate oregano material and 100% of the particulate tobacco material may have diameters of less than or equal to about 300 microns, more preferably less than or equal to about 275 microns. The particle size range of the Oregano particles enables the Oregano particles to be combined with tobacco particles in existing cast leaf processes.

The homogenized oregano material preferably comprises at least about 55 weight percent of the particulate plant material comprising oregano particles as described above, more preferably at least about 60 weight percent of the particulate plant material, and even more preferably at least about 65 weight percent of the particulate plant material, on a dry weight basis. The homogenized oregano material preferably comprises no more than about 95 weight percent particulate plant material, more preferably no more than about 90 weight percent particulate plant material, and even more preferably no more than about 85 weight percent particulate plant material on a dry weight basis. For example, the homogenized oregano material may comprise from about 55 weight percent to about 95 weight percent particulate plant material, or from about 60 weight percent to about 90 weight percent particulate plant material, or from about 65 weight percent to about 85 weight percent particulate plant material, on a dry weight basis. In a particularly preferred embodiment, the homogenized oregano material comprises about 75 weight percent particulate plant material on a dry weight basis.

Thus, the particulate plant material is typically combined with one or more other components to form a homogenized oregano material.

As defined above, the homogenized oregano material further comprises an aerosol former. Upon volatilization, the aerosol-forming agent can deliver other vaporized compounds in the aerosol that are released from the aerosol-generating substrate upon heating, such as nicotine and flavoring agents. Aerosolization of a particular compound from an aerosol-generating substrate is not solely determined by its boiling point. The amount of aerosolized compound can be affected by the physical form of the matrix as well as other components that are also present in the matrix. The stability of a compound at the temperature and time range of aerosolization will also affect the amount of compound present in the aerosol.

Suitable aerosol formers included in homogenized oregano material are known in the art and include, but are not limited to: polyhydric alcohols such as triethylene glycol, propylene glycol, 1, 3-butanediol and glycerol; esters of polyhydric alcohols, such as monoacetin, diacetin or triacetin; and aliphatic esters of mono-, di-or polycarboxylic acids, such as dimethyl dodecanedioate and dimethyl tetradecanedioate. The homogenized oregano material may comprise a single aerosol former or a combination of two or more aerosol formers.

The homogenized oregano material preferably has an aerosol former content of about 5 wt% to about 30 wt% on a dry weight basis, such as about 10 wt% to about 25 wt% on a dry weight basis, or about 15 wt% to about 20 wt% on a dry weight basis.

For example, if the substrate is intended for use in an aerosol-generating article of an electrically operated aerosol-generating system having a heating element, it may preferably comprise an aerosol-former content of between about 5% and about 30% by weight on a dry weight basis. If the substrate is intended for use in an aerosol-generating article of an electrically operated aerosol-generating system having a heating element, the aerosol-former is preferably glycerol.

In other embodiments, the homogenized oregano material may have an aerosol former content of about 1% to about 5% by weight on a dry weight basis. For example, if the substrate is intended for use in an aerosol-generating article in which the aerosol-former is held in a reservoir separate from the substrate, the substrate may have an aerosol-former content of greater than 1% and less than about 5%. In such embodiments, the aerosol-forming agent volatilizes upon heating and the stream of aerosol-forming agent contacts the aerosol-generating substrate so as to entrain flavour from the aerosol-generating substrate in the aerosol.

The aerosol-former may act as a humectant in the aerosol-generating substrate.

Alternatively or additionally, the homogenized oregano material may further comprise an acid. The acid may comprise a carboxylic acid. The carboxylic acid may include a ketone group. Preferably, the carboxylic acid may comprise a ketone group having less than about 10 carbon atoms, or less than about 6 carbon atoms, or less than about 4 carbon atoms, such as levulinic acid or lactic acid. As described below, the inclusion of an acid may be particularly advantageous when the aerosol-generating substrate is in the form of a gel.

As defined above, the homogenized oregano material further comprises a binder to alter the mechanical properties of the particulate plant material, wherein the binder is included in the homogenized oregano material during manufacture as described herein. Suitable exogenous adhesives are known to those skilled in the art and include, but are not limited to: gums such as guar gum, xanthan gum, acacia gum and locust bean gum; cellulosic binders such as hydroxypropyl cellulose, carboxymethyl cellulose, hydroxyethyl cellulose, methyl cellulose, and ethyl cellulose; polysaccharides, such as starch; organic acids such as alginic acid; conjugate base salts of organic acids such as sodium alginate, agar and pectin; and combinations thereof. Preferably, the binder comprises guar gum.

Preferably, the binder is present in an amount of about 1 wt% to about 10 wt% based on the dry weight of the homogenized oregano material, preferably in an amount of about 2 wt% to about 5wt% based on the dry weight of the homogenized oregano material.

In addition, the homogenized oregano material may also optionally include one or more lipids to facilitate diffusion of volatile components (e.g., aerosol former, (E) -anethole, and nicotine), wherein the lipids are included in the homogenized oregano material during manufacture as described herein. Suitable lipids included in the homogenized plant material include, but are not limited to: medium chain triglycerides, cocoa butter, palm oil, palm kernel oil, mango oil, shea butter, soybean oil, cottonseed oil, coconut oil, hydrogenated coconut oil, candelilla wax, carnauba wax, shellac, sunflower wax, sunflower oil, rice bran and Revel A; and combinations thereof.

Alternatively or additionally, the homogenized oregano material may further comprise a pH adjuster.

Alternatively or additionally, the homogenized oregano material may further comprise reinforcing fibers to alter the mechanical properties of the homogenized oregano material, wherein the reinforcing fibers are included in the homogenized oregano material during manufacture as described herein. Suitable exogenous fibers for inclusion in the homogenized oregano material are known in the art and include fibers formed from non-tobacco materials and non-oregano materials, including, but not limited to: cellulose fibers; cork fiber; a hardwood fiber; jute fibers and combinations thereof. Exogenous fibers derived from tobacco and/or oregano may also be added. Any fibers added to the homogenized oregano material are not considered to form part of the "particulate plant material" as defined above. Prior to inclusion in the homogenized oregano material, the fibers may be treated by suitable methods known in the art including, but not limited to: mechanical pulping; refining; chemical pulping; bleaching; pulping by sulfate; and combinations thereof. The fibers typically have a length that is greater than their width.

Suitable fibers generally have a length greater than 400 microns and less than or equal to 4mm, preferably in the range of 0.7mm to 4 mm. Preferably, the fibers are present in an amount of at least about 2% by weight based on the dry weight of the matrix. The amount of fibers in the homogenized oregano material may depend on the type of material and, in particular, the method used to produce the homogenized oregano material. In some embodiments, the fibers may be present in an amount of about 2 wt% to about 15 wt%, most preferably about 4 wt%, based on the dry weight of the matrix. For example, when the homogenized plant material is in the form of cast leaves, such levels of fibers may be present. In other embodiments, the fibers may be present in an amount of at least about 30 wt% or at least about 40 wt%. For example, when the homogenized oregano material is oregano paper formed during a papermaking process, it is likely that such higher levels of fibers will be provided.

In a preferred embodiment of the invention, the homogenized oregano material comprises oregano particles, about 5 wt% to about 30 wt% aerosol former, and about 1wt% to about 10 wt% binder on a dry weight basis. In such embodiments, the homogenized oregano material preferably further comprises from about 2 weight percent to about 15 weight percent fiber. Particularly preferably, the binder is guar gum.

The homogenized plant material of the aerosol-generating substrate according to the invention may comprise a single type of homogenized plant material or two or more types of homogenized plant material having different compositions or forms from each other. For example, in one embodiment, the aerosol-generating substrate comprises oregano particles and tobacco particles contained within the same sheet of homogenized plant material. However, in other embodiments, the aerosol-generating substrate may comprise tobacco particles and oregano particles within different sheets from each other.

The homogenized oregano material is preferably in the form of a solid or gel. However, in some embodiments, the homogenized material may be in a solid form that is not a gel. Preferably, the homogenising material is not in the form of a film.

The homogenized plant material may be provided in any suitable form. For example, the homogenized oregano material may be in the form of one or more sheets. As used herein with reference to the present invention, the term "sheet" describes a layered element having a width and length substantially greater than its thickness.

Alternatively or additionally, the homogenized oregano material may be in the form of a plurality of pellets or granules.

Alternatively or additionally, the homogenized oregano material may be in a form that is capable of being filled with cartridges or hookah consumables, or may be in a form that is capable of being used in a hookah apparatus. The invention includes a cartridge or hookah apparatus containing homogenized oregano material.

Alternatively or additionally, the homogenized oregano material may be in the form of a plurality of strips, ribbons, or pieces. As used herein, the term "strand" describes an elongated element material having a length substantially greater than its width and thickness. The term "sliver" shall be considered to include strips, pieces and any other homogenized oregano material having a similar form. The thin strips of homogenized oregano material may be formed from a sheet of homogenized oregano material, such as by cutting or shredding, or by other methods, such as by extrusion methods.

In some embodiments, the thin strips may be formed in situ within the aerosol-generating substrate due to splitting or splitting of the sheet of homogenized oregano material during formation of the aerosol-generating substrate, for example due to crimping. The thin strips of homogenized oregano material within the aerosol-generating substrate may be separated from one another. Alternatively, each sliver of homogenized oregano material within an aerosol-generating substrate may be connected to adjacent one or more slivers at least partially along the length of the sliver. For example, adjacent strands may be connected by one or more fibers. This may occur, for example, in the case of the formation of thin strips due to the splitting of sheets of homogenized oregano material during the production of an aerosol-generating substrate, as described above.

Preferably, the aerosol-generating substrate is in the form of one or more sheets of homogenized oregano material. In various embodiments of the invention, one or more sheets of homogenized oregano material may be produced by a casting process. In various embodiments of the invention, one or more sheets of homogenized oregano material may be produced by a papermaking process. The one or more sheets as described herein may each individually have a thickness of 100 micrometers to 600 micrometers, preferably 150 micrometers to 350 micrometers, most preferably 200 micrometers to 250 micrometers. The individual thickness refers to the thickness of the individual sheets, while the combined thickness refers to the total thickness of all sheets constituting the aerosol-generating substrate. For example, if the aerosol-generating substrate is formed from two separate sheets, the combined thickness is the sum of the thicknesses of the two separate sheets or the measured thickness of the two sheets in case the two sheets are stacked in the aerosol-generating substrate.

The one or more sheets as described herein may each individually have a grammage of about 100g/m ² to about 300g/m ².

One or more sheets described herein may each individually have a density of about 0.3g/cm ³ to about 1.3g/cm ³, preferably about 0.7g/cm ³ to about 1.0g/cm ³.

The term "tensile strength" is used throughout the specification to denote a measure of the force required to stretch a sheet of homogenized oregano material until it breaks. More specifically, tensile strength is the maximum tensile force per unit width that a sheet material will withstand before breaking, and is measured in the machine or transverse direction of the sheet material. The unit of tensile strength is expressed in newtons per meter (N/m). Methods for measuring the tensile strength of a sheet are well known. Suitable tests are described in International Standard ISO 1924-2, published 2014, entitled "Paper and Board-Determination of Tensile Properties-section 2: constant Rate of Elongation Method".

The materials and equipment required for testing according to ISO 1924-2 are: a universal tensile/compression tester, instron 5566, or equivalent; a 100 newton, instron or equivalent tension load cell; two pneumatic clamps; a steel gauge block 180+ -0.25 mm long (width: about 10 mm, thickness: about 3 mm); a double blade slitter sized 15±0.05 x about 250 mm, adamel Lhomargy, or equivalent; a surgical knife; running a computer that collects the software Merlin, or equivalent; and compressed air.

Samples were prepared by first conditioning a sheet of homogenized oregano material at 22±2 degrees celsius and 60±5% relative humidity for at least 24 hours prior to testing. The longitudinal or transverse samples were then cut to about 250 x 15 ± 0.1 mm using a double blade slitter. The edges of the test pieces must be cleanly cut, so that no more than three test pieces are cut at the same time.

The tensile/compressive test instrument was set by installing a 100 newton tension load cell, switching on a general purpose tensile/compressive tester and computer, and selecting a predetermined measurement method in software, wherein the test speed was set to 8 millimeters per minute. The tension load cell is then calibrated and the pneumatic clamp is installed. The test distance between the pneumatic clamps was adjusted to 180±0.5 mm by a steel gauge block and the distance and force were set to zero.

The specimen is then placed straight in the center between the clamps and touching the area to be tested with a finger is avoided. The upper clamp is closed and the strip is suspended in the open lower clamp. The force is set to zero. Then lightly pulling down the paper strip, and closing the lower clamp; the initial force must be between 0.05 newton and 0.20 newton. As the upper clamp moves upward, a progressively increasing force is applied until the specimen breaks. The same procedure was repeated for the remaining samples. When the clamps are separated by a distance of more than 10mm, the results are valid when the specimen breaks. If this is not the case, the result is rejected and additional measurements are performed.

As described above, if the test sample of available homogenized oregano material is smaller than the sample described in the test conducted according to ISO 1924-2, the test can be easily scaled down to accommodate the available size of the test sample.

One or more sheets of homogenized oregano material as described herein may each individually have a peak tensile strength in the cross direction of 50N/m to 400N/m, or preferably 150N/m to 350N/m. In view of the sheet thickness affecting tensile strength, and where a batch of sheets exhibits thickness variation, it may be desirable to normalize the value to a particular sheet thickness.

One or more sheets as described herein may each individually have a peak tensile strength in the machine direction of 100N/m to 800N/m or preferably 280N/m to 620N/m, normalized to a sheet thickness of 215 μm. The machine direction refers to the direction in which sheet material will be wound onto or unwound from a roll and fed into the machine, while the transverse direction is perpendicular to the machine direction. Such tensile strength values make the sheets and methods described herein particularly suitable for subsequent operations involving mechanical stress.

Providing a sheet having the thickness, grammage and tensile strength levels as defined above advantageously optimizes the machinability of the sheet to form an aerosol-generating substrate and ensures that damage, such as tearing of the sheet, is avoided during high speed processing of the sheet.

In embodiments of the invention in which the aerosol-generating substrate comprises one or more sheets of homogenized oregano material, the sheets are preferably in the form of one or more aggregated sheets. As used herein, the term "gathering" means that a sheet of homogenized oregano material is rolled, folded or otherwise compressed or contracted to be substantially transverse to the cylindrical axis of the rod or bar. The step of "gathering" the sheet material may be performed by any suitable means that provides the necessary lateral compression of the sheet material.

As used herein, the term "longitudinal" refers to a direction corresponding to a major longitudinal axis of the aerosol-generating article extending between an upstream end and a downstream end of the aerosol-generating article. During use, air is drawn through the aerosol-generating article in a longitudinal direction. The term "transverse" refers to a direction perpendicular to the longitudinal axis. As used herein, the term "length" refers to the dimension of a component in the longitudinal direction, and the term "width" refers to the dimension of a component in the transverse direction. For example, in the case of a rod or bar having a circular cross-section, the maximum width corresponds to the diameter of the circle.

As used herein, the term "rod" means a generally cylindrical element having a substantially polygonal, circular, oval or elliptical cross-section. As used herein, the term "strip" refers to a generally cylindrical element having a generally polygonal cross-section and preferably having a circular, oval or elliptical cross-section. The length of the bar may be greater than or equal to the length of the rod. Typically, the length of the bar is greater than the length of the bar. The strip may comprise one or more bars, preferably aligned longitudinally.

As used herein, the terms "upstream" and "downstream" describe the relative positions of an element or portion of an element of an aerosol-generating article with respect to the direction in which an aerosol is transported through the aerosol-generating article during use. The downstream end of the airflow path is the end of the aerosol that is delivered to the user of the article.

One or more sheets of homogenized oregano material may be gathered transversely with respect to its longitudinal axis and surrounded with a wrapper to form a continuous strip or rod. The continuous rod may be cut into a plurality of discrete rods or sticks. The wrapper may be a paper wrapper or a non-paper wrapper, as described in more detail below.

Alternatively, one or more sheets of homogenized oregano material may be cut into thin strips as described above. In such embodiments, the aerosol-generating substrate comprises a plurality of strips of homogenized oregano material. The thin strips may be used to form a rod. Typically, such strips have a width of at least about 0.2mm, or at least about 0.5mm. Typically, such strips have a width of no more than about 5mm, or about 4mm, or about 3mm, or about 1.5mm. For example, the width of the strands may be between about 0.25mm to about 5mm, or between about 0.25mm to about 3mm, or between about 0.5mm to about 1.5mm.

The length of the strand is preferably greater than about 5mm, for example between about 5mm and about 20mm, or between about 8mm and about 15mm, or about 12mm. Preferably, the strips have substantially the same length as each other. The length of the thin strip may be determined by the manufacturing process whereby the strip is cut into shorter bars and the length of the thin strip corresponds to the length of the bars. The strands may be fragile, which may lead to breakage, especially during transportation. In this case, some of the strands may be less in length than the rod.

The plurality of strips preferably extend substantially longitudinally along the length of the aerosol-generating substrate in alignment with the longitudinal axis. Preferably, the plurality of strips are thus aligned substantially parallel to each other. The plurality of longitudinal strips of aerosol-generating material are preferably substantially non-crimped.

The strands of homogenized oregano material preferably each have a mass surface area ratio of at least about 0.02 milligrams per square millimeter, more preferably at least about 0.05 milligrams per square millimeter. Preferably, the strands of homogenized oregano material each have a mass surface area ratio of no more than about 0.2 milligrams per square millimeter, more preferably no more than about 0.15 milligrams per square millimeter. The mass-to-surface area ratio is calculated by dividing the mass (in milligrams) of the thin strips of homogenized oregano material by the geometric surface area (in square millimeters) of the thin strips of homogenized oregano material.

One or more sheets of homogenized oregano material may be textured by crimping, embossing, or perforating. One or more sheets may be textured prior to gathering or prior to cutting into thin strips. Preferably, one or more sheets of homogenized oregano material are crimped prior to aggregation, such that the homogenized oregano material may be in the form of crimped sheets, more preferably in the form of aggregated crimped sheets. As used herein, the term "crimped sheet" refers to a sheet having a plurality of substantially parallel ridges or corrugations that are generally aligned with the longitudinal axis of the article.

In one embodiment, the aerosol-generating substrate may be in the form of a single rod of aerosol-generating substrate. Preferably, the rod of aerosol-generating substrate may comprise a plurality of strips of homogenized oregano material. Most preferably, the rod of aerosol-generating substrate may comprise one or more sheets of homogenized oregano material. Preferably, one or more sheets of homogenized oregano material may be crimped such that it has a plurality of ridges or corrugations that are substantially parallel to the cylindrical axis of the rod. This treatment advantageously promotes the aggregation of the curled sheet of homogenized oregano material to form a rod. Preferably, one or more sheets of homogenized oregano material may be gathered. It will be appreciated that the curled sheet of homogenised oregano material may alternatively or additionally have a plurality of substantially parallel ridges or corrugations disposed at an acute or obtuse angle to the cylindrical axis of the rod. The sheet may be curled to such an extent that the integrity of the sheet is compromised at the plurality of parallel ridges or corrugations, causing the material to separate and resulting in the formation of fragments, strips or ribbons of homogenized oregano material.

In another embodiment of the aerosol-generating substrate, the homogenized plant material comprises a first rod comprising a first homogenized plant material and a second rod comprising a second homogenized plant material, wherein said first homogenized plant material and said second homogenized plant material comprise different levels of oregano particles and tobacco particles. For example, the first homogenized plant material may comprise from about 50 weight percent to about 75 weight percent of oregano particles on a dry weight basis; and the second homogenized plant material comprises from about 50 weight percent to about 75 weight percent tobacco particles on a dry weight basis. In summary, according to the present invention, the homogenized plant material within the aerosol generating substrate preferably comprises at least 2.5 wt.% oregano particles and at most 70 wt.% tobacco particles on a dry weight basis.

In such an arrangement, the first homogenized plant material preferably comprises a first particulate plant material having a higher proportion of oregano particles than the second homogenized plant material. The second homogenized plant material may be homogenized tobacco material that is substantially free of oregano particles.

Preferably, the first homogenized plant material may be in the form of one or more sheets and the second homogenized plant material may be in the form of one or more sheets.

Optionally, the aerosol-generating substrate may comprise one or more rods. Preferably, the matrix may comprise a first rod and a second rod, wherein the first homogenized plant material may be located in the first rod and the second homogenized plant material may be located in the second rod.

Two or more bars may be combined in abutting end-to-end relationship and extended to form a strip. The two bars may be placed longitudinally with a gap between them, creating a cavity within the bar. The bars may be in any suitable arrangement within the strip.

For example, in one preferred arrangement, a downstream rod comprising a major proportion of oregano particles may abut an upstream rod comprising a major proportion of tobacco particles to form a rod. Alternative configurations are also contemplated in which the upstream and downstream positions of the respective bars are changed relative to each other. Alternative configurations are also contemplated wherein the third homogenized plant material contains different proportions of oregano particles and tobacco particles and forms a third rod. In the case where two or more sticks are provided, the homogenized plant material may be provided in each stick in the same form, or in different forms, i.e. aggregated or chopped. One or more rods may optionally be individually or together wrapped in the thermally conductive sheet material as described below.

The first rod may comprise one or more sheets of first homogenized plant material and the second rod may comprise one or more sheets of second homogenized plant material. The sum of the lengths of the rods may be between about 10mm and about 40mm, preferably between about 10mm and about 15mm, more preferably about 12mm. The first rod and the second rod may have the same length or may have different lengths. If the first and second bars have the same length, the length of each bar may preferably be about 6mm to about 20mm. Preferably, the second rod may be longer than the first rod so as to provide a desired ratio of tobacco particles to oregano particles in the matrix. In summary, it is preferred that the matrix contains from 0wt% to 75 wt% tobacco particles and from 0.5wt% to 75 wt% Oregano particles on a dry weight basis. Preferably, the second rod is at least 40% to 50% longer than the first rod.

If the first homogenized plant material and the second homogenized plant material are in the form of one or more sheets, preferably the one or more sheets of first homogenized plant material and second homogenized plant material may be aggregated sheets. Preferably, the one or more sheets of first homogenized plant material and second homogenized plant material may be crimped sheets. It should be understood that all other physical properties described with reference to embodiments in which a single homogenized plant material is present are equally applicable to embodiments in which a first homogenized plant material and a second homogenized plant material are present. Furthermore, it should be understood that the description of additives (e.g., binders, lipids, fibers, aerosol formers, humectants, plasticizers, flavoring agents, fillers, aqueous and non-aqueous solvents, and combinations thereof) with reference to embodiments in which a single homogenized plant material is present applies equally to embodiments in which a first homogenized plant material and a second homogenized plant material are present.

In yet another embodiment of the aerosol-generating substrate, the first homogenized plant material is in the form of a first sheet, the second homogenized plant material is in the form of a second sheet, and the second sheet at least partially covers the first sheet.

The first sheet may be a textured sheet and the second sheet may be non-textured.

Both the first sheet and the second sheet may be textured sheets.

The first sheet may be a textured sheet that is textured in a different manner than the second sheet. For example, the first sheet may be crimped while the second sheet may be perforated. Or the first sheet may be perforated and the second sheet may be crimped.

The first sheet and the second sheet may each be a crimp sheet that is morphologically different from each other. For example, the second sheet may be crimped with a different amount of crimp per unit width of sheet than the first sheet.

These sheets may be gathered to form a rod. The sheets that are gathered together to form the rod may have different physical dimensions. The width and thickness of the sheet material may vary.

It may be desirable to gather two sheets together, each having a different thickness or each having a different width. This may change the physical properties of the rod. This may facilitate the formation of a blend rod of aerosol-generating substrates from sheets of different chemical compositions.

The first sheet may have a first thickness and the second sheet may have a second thickness that is a multiple of the first thickness, e.g., the second sheet may have a thickness that is two or three times the first thickness.

The first sheet may have a first width and the second sheet may have a second width different from the first width.

The first sheet and the second sheet may be disposed in overlapping relationship prior to or at the point at which they are brought together. The sheets may have the same width and thickness. The sheets may have different thicknesses. The sheets may have different widths. The sheets may have different textures.

Where it is desired that both the first and second sheets be textured, the sheets may be textured simultaneously prior to gathering. For example, the sheets may be brought into overlapping relationship and passed through a texturing device, such as a pair of crimping rollers. A suitable apparatus and method for simultaneous crimping is described with reference to figure 2 of WO-A-2013/178766. In a preferred embodiment, the second sheet of second homogenized plant material covers the first sheet of first homogenized plant material and the combined sheets are gathered to form a rod of aerosol generating substrate. Optionally, the sheets may be crimped together prior to gathering to facilitate gathering.

Or each sheet may be textured separately and then subsequently put together to aggregate into a rod. For example, in the case where the two sheets have different thicknesses, it may be desirable to press-bond the first sheet differently relative to the second sheet.

It should be understood that all other physical properties described with reference to embodiments in which a single homogenized plant material is present are equally applicable to embodiments in which a first homogenized plant material and a second homogenized plant material are present. Furthermore, it should be understood that the description of additives (e.g., binders, lipids, fibers, aerosol formers, humectants, plasticizers, flavoring agents, fillers, aqueous and non-aqueous solvents, and combinations thereof) with reference to embodiments in which a single homogenized plant material is present applies equally to embodiments in which a first homogenized plant material and a second homogenized plant material are present.

The homogenized plant material for use in an aerosol-generating substrate according to the invention may be produced by a variety of methods, including papermaking, casting, mass reconstruction, extrusion or any other suitable process.

Preferably, the homogenized oregano material is in the form of "cast leaves". The term "cast leaf" is used herein to refer to a sheet product made by a casting process that is based on casting a slurry comprising plant particles (e.g., oregano particles or a mixture of tobacco particles and oregano particles) and a binder (e.g., guar gum) onto a support surface such as a belt conveyor, drying the slurry, and removing the dried sheet from the support surface. Examples of casting or cast leaf processes are described in, for example, US-se:Sup>A-5,724,998 for the manufacture of cast leaf tobacco. In the cast leaf process, particulate plant material is mixed with a liquid component (typically water) to form a slurry. Other additional components in the slurry may include fibers, binders, and aerosol formers. The particulate plant material may agglomerate in the presence of a binder. The slurry is cast onto a support surface and dried to form a homogenized sheet of oregano material.

In certain preferred embodiments, the homogenized oregano material used in the article according to the invention is produced by casting. The homogenized oregano material prepared by a casting process typically includes agglomerated particulate plant material.

In the cast leaf process, most of the flavoring agent is advantageously preserved because substantially all of the soluble fraction remains in the plant material. In addition, energy intensive papermaking steps are avoided.

In a preferred embodiment of the invention, to form a homogenized oregano material, a mixture is formed that comprises particulate plant material, water, a binder, and an aerosol former. A sheet is formed from the mixture and then dried. Preferably, the mixture is an aqueous mixture. As used herein, "dry weight" refers to the weight of a particular nonaqueous component relative to the sum of the weights of all nonaqueous components in the mixture, expressed as a percentage. The composition of the aqueous mixture may be expressed in terms of "dry weight percent". This means that the non-aqueous component is expressed as a percentage relative to the weight of the entire aqueous mixture.

The mixture may be a slurry. As used herein, a "slurry" is a homogenized aqueous mixture having a relatively low dry weight. The slurry used in this method preferably has a dry weight of 5% to 60%.

Or the mixture may be a mass. As used herein, a "briquette" is an aqueous mixture having a relatively high dry weight. The agglomerates used in the methods herein preferably have a dry weight of at least 60%, more preferably at least 70%.

In certain embodiments of the method of the present invention, it is preferred to include greater than 30% dry weight of the slurry and agglomerates.

The step of mixing the particulate plant material, water and other optional components may be carried out by any suitable method. For low viscosity mixtures, i.e. some slurries, it is preferred to use a high energy mixer or a high shear mixer for mixing. This mixing breaks down and evenly distributes the phases of the mixture. For higher viscosity mixtures, i.e. some agglomerates, a kneading process can be used to uniformly distribute the various phases of the mixture.

The method according to the invention may further comprise the step of vibrating the mixture to dispense the various components. Vibrating the mixture, i.e. for example vibrating a tank or silo in which the homogenized mixture is present, may assist in the homogenization of the mixture, especially when the mixture is a low viscosity mixture, i.e. some slurries. If vibration and mixing are performed, less mixing time may be required to homogenize the mixture to the target value optimal for casting.

If the mixture is a slurry, the web of homogenized oregano material is preferably formed by a casting process that includes casting the slurry on a support surface such as a belt conveyor. A method for producing a homogenized oregano material includes the step of drying the cast web to form a sheet. The cast web may be dried at room temperature or at an ambient temperature of at least about 60 degrees celsius, more preferably at least about 80 degrees celsius for a suitable length of time. Preferably, the cast web is dried at an ambient temperature of no more than 200 degrees celsius, more preferably no more than about 160 degrees celsius. For example, the cast web may be dried at a temperature between about 60 degrees celsius and about 200 degrees celsius, or between about 80 degrees celsius and about 160 degrees celsius. Preferably, the moisture content of the dried sheet is between about 5% and about 15% based on the total weight of the sheet. Then, after drying, the sheet may be removed from the support surface. The cast sheet has a tensile strength such that it can be mechanically handled and wound or unwound from a roll without breaking or deforming.

If the mixture is a pellet, the pellet may be extruded in the form of a sheet, strand or ribbon prior to the step of drying the extruded mixture. Preferably, the mass can be extruded in the form of a sheet. The extrusion mixture may be dried at room temperature or at a temperature of at least about 60 degrees celsius, more preferably at least about 80 degrees celsius, for a suitable length of time. Preferably, the extrusion mixture is dried at an ambient temperature of no more than 200 degrees celsius, more preferably no more than about 160 degrees celsius. For example, the extrusion mixture may be dried at a temperature between about 60 degrees celsius and about 200 degrees celsius, or between about 80 degrees celsius and about 160 degrees celsius. Preferably, the moisture content of the extruded mixture after drying is between about 5% to about 15% based on the total weight of the sheet. Sheets formed from the agglomerates require less drying time and/or lower drying temperature because of the significantly lower moisture content relative to webs formed from the slurry.

After the sheet has been dried, the method may optionally comprise the step of applying A nicotine salt, preferably together with an aerosol former, to the sheet, as described in WO-A-2015/082652.

After the sheet has been dried, the method according to the invention may optionally comprise the step of cutting the sheet into strips, fragments or ribbons for forming an aerosol-generating substrate as described above. The strips, fragments or ribbons may be brought together using suitable means to form a ribbon of aerosol-generating substrate. In the formed strips of aerosol-generating substrate, the thin strips, fragments or strips may for example be substantially aligned in the longitudinal direction of the strips. Or the thin strips, fragments or ribbons may be randomly oriented in the ribbon.

The method according to the invention may optionally further comprise the step of winding the sheet onto a reel after the drying step.

The present invention also provides an alternative papermaking process for producing homogenized plant material sheet in the form of plant "paper". Plant paper refers to reconstituted plant sheet formed by a process in which plant material is extracted with a solvent to produce an extract of soluble plant compounds and insoluble residues of fibrous plant material and the extract is recombined with the insoluble residues. The extract may optionally be concentrated or further processed before being recombined with the insoluble residue. The insoluble residue may optionally be refined and combined with additional plant fibers prior to recombination with the extract. In the method according to the invention, the plant material will comprise oregano particles, optionally in combination with tobacco particles.

In more detail, the method of producing a plant paper comprises a first step of mixing plant material and water to form a dilute suspension. The dilute suspension mainly comprises individual cellulose fibers. The suspension has a lower viscosity and a higher water content than the slurry produced in the casting process. This first step may comprise soaking, optionally in the presence of a base such as sodium hydroxide, and optionally applying heat.

The method further comprises a second step of separating the suspension into an insoluble fraction comprising insoluble residues of the fibrous plant material and a liquid or aqueous extract comprising soluble plant compounds. The water remaining in the insoluble residues of the fibrous plant material can be drained through the screen acting as a sieve, so that a web of randomly interwoven fibers can be laid down. The water may be further removed from this web by pressing with rollers, sometimes with suction or vacuum assistance.

After removal of the aqueous portion and water, insoluble residues are formed into sheets. Preferably, a substantially flat, uniform sheet of plant fibers is formed.

Preferably, the method further comprises the steps of concentrating the extract of soluble plant compounds removed from the sheet and adding the concentrated extract to the sheet of insoluble residues of fibrous plant material to form a sheet of homogenized plant material. Alternatively or additionally, soluble plant material or concentrated plant material from another method may be added to the sheet. The extract or concentrated extract may be from another variety of the same plant species or from another plant species.

Such se:Sup>A process has been used with tobacco to manufacture reconstituted tobacco products, also known as tobacco paper, as described in US-se:Sup>A-3,860,012. The same method can also be used for one or more plants to produce paper-like sheet materials, such as sheets of oregano paper.

In certain preferred embodiments, the homogenized plant material for use in articles according to the invention is produced by a papermaking process as defined above. In such embodiments, the homogenized oregano material is in the form of oregano paper.

The homogenized tobacco material or homogenized oregano material produced by such a process is referred to as tobacco paper or oregano paper. Homogenized plant material made by the papermaking process may be distinguished by the presence of a large number of fibers in the overall material that are visible to the naked eye or under an optical microscope, particularly when the paper is wetted with water. In contrast, homogenized plant material produced by a casting process contains less fibers than paper and tends to dissociate into a slurry when it is wetted. Blended tobacco Oregano paper refers to homogenized plant material produced by this method using a mixture of tobacco and Oregano material.

In embodiments in which the aerosol-generating substrate comprises a combination of oregano particles and tobacco particles, the aerosol-generating substrate may comprise one or more sheets of oregano paper and one or more sheets of tobacco paper. The sheets of oregano paper and tobacco paper may be interleaved or stacked with each other prior to being gathered to form a strip. Optionally, the sheet may be crimped. Alternatively, sheets of oregano paper and tobacco paper may be cut into strips, ribbons, or pieces and then combined to form the strips. The relative amounts of tobacco and oregano in the aerosol-generating substrate may be adjusted by varying the respective numbers of tobacco and oregano sheets or the respective amounts of oregano and tobacco strips, bands or fragments in the rod.

For example, the number or amount of tobacco and oregano sheets or strips may be adjusted to provide an oregano to tobacco ratio of about 1:4, or about 1:9, or about 1:30.

Other known processes that may be suitable for producing homogenized plant material are, for example, the briquette reconstruction process of the type described in US-se:Sup>A-3,894,544; and extrusion processes of the type described in, for example, GB-A-983,928. Generally, the density of the homogenized plant material produced by the extrusion process and the mass reconstruction process is greater than the density of the homogenized plant material produced by the casting process.

In an alternative embodiment of the invention, the homogenized oregano material is in the form of a gel composition formed from oregano particles, an aerosol former, and a binder.

Preferably, when the homogenized oregano material is in the form of a gel composition containing oregano particles, the binder comprises a cellulose ether such as carboxymethyl cellulose. The binder may be present in an amount of about 1 wt% to about 5 wt% based on the total weight of the gel. For example, the gel composition may comprise 1.5 wt% to 3.5 wt% sodium carboxymethyl cellulose.

Preferably, the gel composition comprises at least about 60wt% of an aerosol former such as glycerol, based on the total weight of the gel. For example, the gel composition may comprise 65% to 85% by weight of glycerol.

Optionally, the gel composition may also comprise an acid, such as lactic acid. The acid may be present in an amount up to about 6 wt% based on the total weight of the gel composition. Optionally, the gel composition may comprise up to about 5 wt% nicotine based on the total weight of the gel composition. Optionally, the gel composition comprises about 10 wt% to about 30 wt% water based on the total weight of the gel composition.

In embodiments in which the homogenized oregano material is in the form of a gel composition, the aerosol-generating substrate preferably comprises a porous medium loaded with the gel composition. The term "porous" is used herein to refer to a material that provides a plurality of pores or openings that allow air to pass through the material.

The porous medium may be any suitable porous material capable of containing or retaining the gel composition. Desirably, the porous medium may allow the gel composition to move within it. In particular embodiments, the porous medium comprises a natural material, a synthetic or semi-synthetic material, or a combination thereof. In particular embodiments, the porous medium comprises a sheet material, foam, or fiber, such as loose fibers; or a combination thereof. In particular embodiments, the porous medium comprises a woven, nonwoven, or extruded material, or a combination thereof. Preferably, the porous medium comprises cotton, paper, viscose, PLA or cellulose acetate, or a combination thereof. Preferably, the porous medium comprises a sheet material, such as cotton or cellulose acetate. In a particularly preferred embodiment, the porous medium comprises a sheet made of cotton fibers.

The porous media used in the present invention may be crimped or chopped. In a preferred embodiment, the porous medium is crimped. In an alternative embodiment, the porous medium comprises a chopped porous medium. The crimping or shredding process may be before or after loading the gel composition.

Preferably, when the homogenized oregano material is in the form of a gel composition loaded onto a porous medium, the aerosol-generating substrate comprises an elongated susceptor element extending longitudinally through or adjacent to the porous medium.

Preferably, the aerosol-generating substrate of the aerosol-generating article according to the invention comprises at least about 200mg of homogenized oregano material, more preferably at least about 250mg of homogenized oregano material, more preferably at least about 275mg of homogenized oregano material.

An aerosol-generating article according to the invention comprises a rod comprising an aerosol-generating substrate in one or more rods. The strips of aerosol-generating substrate may have a length of from about 5mm to about 120 mm. For example, the strip may preferably have a length of about 10mm to about 45mm, more preferably about 10mm to 15mm, most preferably about 12 mm. In alternative embodiments, the strips preferably have a length of about 30mm to about 45mm, or about 33mm to about 41 mm. When the rod is formed from a single rod of aerosol-generating substrate, the rod has the same length as the rod.

The strips of aerosol-generating substrate may have an outer diameter of from about 5mm to about 10mm, depending on their intended use. For example, in some embodiments, the strips may have an outer diameter of about 5.5mm to about 8mm, or about 6.5mm to about 8 mm. The outer diameter of the strip of aerosol-generating substrate corresponds to the diameter of the strip including any wrapper.

The strip of aerosol-generating substrate of the aerosol-generating article according to the invention is preferably surrounded along at least a portion of its length by one or more wrappers. The one or more packages may include paper packages or non-paper packages or both. Suitable paper packages for use in certain embodiments of the present invention are known in the art and include, but are not limited to: cigarette paper; and a filter segment wrapper. Suitable non-paper packages for use in certain embodiments of the invention are known in the art and include, but are not limited to, sheets of homogenized tobacco material. Homogenized tobacco packages are particularly suitable for use in embodiments in which the aerosol-generating substrate comprises one or more sheets of homogenized oregano material formed from particulate plant material that contains oregano particles in combination with a low weight percentage of tobacco particles, such as 20 to 0 weight percent tobacco particles on a dry weight basis.

In certain embodiments of the invention, the aerosol-generating substrate is surrounded along at least a portion of its length by a thermally conductive sheet material, for example a metal foil such as aluminium foil or metallised paper. The metal foil or metallized paper is used for the purpose of rapid thermal conduction throughout the aerosol-generating substrate. In addition, metal foil or metallized paper may be used to prevent ignition of the aerosol-generating substrate in the event that a consumer attempts to ignite it. Furthermore, during use, the metal foil or metallized paper may prevent odors generated when the outer package is heated from entering the aerosol generated by the aerosol-generating substrate. For example, this may be a problem for aerosol-generating articles having an aerosol-generating substrate that is heated from the outside during use to generate an aerosol. Alternatively or additionally, the metallized wrapper may be used to facilitate detection or identification of the aerosol-generating article when the aerosol-generating article is inserted into the aerosol-generating device during use. The metal foil or metallized paper may comprise metal particles, such as iron particles.

The one or more packages surrounding the aerosol-generating substrate preferably have a total thickness of about 0.1mm to about 0.9 mm.

The inner diameter of the strips of aerosol-generating substrate is preferably between about 3mm and about 9.5mm, more preferably between about 4mm and about 7.5mm, more preferably between about 5mm and about 7.5 mm. The "inner diameter" corresponds to the diameter of the strip of aerosol-generating substrate, excluding the thickness of the wrapper, but the wrapper is still in place when measured. Aerosol-generating articles according to the invention also include, but are not limited to, cartridges or hookah consumables.

The aerosol-generating article according to the invention may optionally comprise a support element comprising at least one hollow tube immediately downstream of the aerosol-generating substrate. One function of the tube is to position the aerosol-generating substrate towards the distal end of the aerosol-generating article such that the aerosol-generating substrate may be in contact with the heating element. The tube is used to prevent the aerosol-generating substrate from being forced along the aerosol-generating article towards other downstream elements when the heating element is inserted into the aerosol-generating substrate. The tube also acts as a spacer element to separate downstream elements from the aerosol-generating substrate. The tube may be made of any material, such as cellulose acetate, polymer, cardboard or paper.

Alternatively or additionally, the aerosol-generating article according to the invention optionally comprises an aerosol-cooling element located downstream of the aerosol-generating substrate and immediately downstream of the hollow tube forming the support element. In use, an aerosol formed from volatile compounds released from the aerosol-generating substrate passes through and is cooled by the aerosol-cooling element and is then inhaled by a user. The lower temperature allows the vapor to condense into an aerosol. The aerosol-cooling element may be a hollow tube, such as a hollow cellulose acetate tube or a cardboard tube, which may be similar to the support element immediately downstream of the aerosol-generating substrate. The aerosol-cooling element may be a hollow tube having an outer diameter equal to the hollow tube forming the support element but an inner diameter smaller or larger than the hollow tube forming the support element. In one embodiment, the aerosol-cooling element wrapped in paper comprises one or more longitudinal channels made of any suitable material, such as metal foil, paper laminated with the foil, polymeric sheet preferably made of synthetic polymer, and substantially non-porous paper or paperboard. In some embodiments, the aerosol-cooling element wrapped in paper may comprise one or more sheets made of a material selected from the group consisting of: polyethylene (PE), polypropylene (PP), polyvinyl chloride (PVC), polyethylene terephthalate (PET), polylactic acid (PLA), cellulose Acetate (CA), paper laminated with polymer sheets, and aluminum foil. Or the aerosol-cooling element may be made of woven or nonwoven filaments of a material selected from the group consisting of Polyethylene (PE), polypropylene (PP), polyvinyl chloride (PVC), polyethylene terephthalate (PET), polylactic acid (PLA) and Cellulose Acetate (CA). In a preferred embodiment, the aerosol-cooling element is a crimped and gathered sheet of polylactic acid encased within filter paper. In another preferred embodiment, the aerosol-cooling element comprises a longitudinal channel and is made of woven filaments of synthetic polymer, such as polylactic acid filaments, which are wrapped in paper.

One or more additional hollow tubes may be provided downstream of the aerosol-cooling element.

The aerosol-generating article according to the invention may further comprise a filter or mouthpiece downstream of the aerosol-generating substrate and, when present, the support element and the aerosol-cooling element. The filter may comprise one or more filter materials to remove particulate components, gaseous components, or a combination thereof. Suitable filter materials are known in the art and include, but are not limited to: fibrous filter materials such as cellulose acetate tow and paper; adsorbents such as activated alumina, zeolite, molecular sieves, and silica gel; biodegradable polymers including, for example, polylactic acid (PLA),Hydrophobic viscose and bioplastic; and combinations thereof. The filter may be located at the downstream end of the aerosol-generating article. The filter may be a cellulose acetate filter segment. In one embodiment, the filter is about 7mm in length, but may have a length of between about 5mm and about 10 mm.

The aerosol-generating article according to the invention may comprise an oral cavity at the downstream end of the article. The mouth end cavity may be defined by one or more wrappers extending downstream from the filter or mouthpiece. Alternatively, the mouth-end cavity may be defined by a separate tubular element arranged at the downstream end of the aerosol-generating article.

The aerosol-generating article according to the invention preferably further comprises a ventilation zone arranged at a position along the aerosol-generating article. For example, the aerosol-generating article may be provided at a location along a hollow tube provided downstream of the aerosol-generating substrate.

The aerosol-generating article according to the invention may optionally further comprise an upstream element at the upstream end of the aerosol-generating substrate. The upstream element may be a porous rod element, such as a rod of fibrous filter material, such as cellulose acetate.

In a preferred embodiment of the invention, the aerosol-generating article comprises an aerosol-generating substrate, at least one hollow tube downstream of the aerosol-generating substrate and a filter downstream of the at least one hollow tube. Optionally, the aerosol-generating article further comprises an oral cavity at the downstream end of the filter. Preferably, the ventilation zone is provided at a location along the at least one hollow tube.

In a particularly preferred embodiment with this arrangement, the aerosol-generating article comprises an aerosol-generating substrate, an upstream element at the upstream end of the aerosol-generating substrate, a support element downstream of the aerosol-generating substrate, an aerosol-cooling element downstream of the support element, and a filter downstream of the aerosol-cooling element. Preferably, the support element and the aerosol-cooling element are both in the form of hollow tubes. Preferably, the aerosol-generating substrate comprises an elongate susceptor element extending longitudinally therethrough.

In a particularly preferred example, the aerosol-generating substrate has a length of about 33mm and an outer diameter of between about 5.5mm and 6.7mm, wherein the aerosol-generating substrate comprises about 340mg of homogenized oregano material in the form of a plurality of strands, wherein the homogenized oregano material comprises about 14 weight percent glycerin on a dry weight basis. In this embodiment, the aerosol-generating article has an overall length of about 74mm and comprises a cellulose acetate tow filter having a length of about 10mm and an oral cavity defined by a hollow tube having a length of about 6-7 mm. The aerosol-generating article comprises a hollow tube downstream of the aerosol-generating substrate, wherein the hollow tube has a length of about 25mm and is provided with a ventilation zone.

The aerosol-generating article according to the invention may have an overall length of at least about 30mm or at least about 40 mm. The total length of the aerosol-generating article may be less than 90mm, or less than about 80mm.

In one embodiment, the aerosol-generating article has an overall length of about 40mm to about 50mm, preferably about 45 mm. In another embodiment, the aerosol-generating article has an overall length of from about 70mm to about 90mm, preferably from about 80mm to about 85 mm. In another embodiment, the aerosol-generating article has an overall length of about 72mm to about 76mm, preferably about 74 mm.

The aerosol-generating article may have an outer diameter of from about 5mm to about 8mm, preferably from about 6mm to about 8 mm. In one embodiment, the aerosol-generating article has an outer diameter of about 7.3 mm.

The aerosol-generating article according to the invention may further comprise one or more aerosol-modifying elements. The aerosol-modifying element may provide an aerosol modifier. As used herein, the term aerosol modifier is used to describe any agent that modifies one or more characteristics or properties of an aerosol passing through a filter in use. Suitable aerosol modifiers include, but are not limited to, agents that impart a taste or aroma to the aerosol passing through the filter in use or agents that remove flavor from the aerosol passing through the filter in use.

The aerosol modifier may be one or more of moisture or a liquid flavoring agent. The water or moisture may alter the sensory experience of the user, for example by wetting the generated aerosol, which may provide a cooling effect to the aerosol and may reduce the irritation experienced by the user. The aerosol modifying element may be in the form of a flavour delivery element for delivering one or more liquid flavours. Alternatively, the liquid flavoring may be added directly to the homogenized oregano material, for example by adding flavor to the slurry or feedstock during the production of the homogenized oregano material, or by spraying the liquid flavoring onto the surface of the homogenized oregano material.

The one or more liquid flavourants may comprise any flavouring compound or plant extract adapted to be releasably disposed in liquid form within the flavour delivery element to enhance the taste of the aerosol generated during use of the aerosol-generating article. Liquid or solid flavoring agents may also be disposed directly in the material forming the filter, such as cellulose acetate tow. Suitable flavors or flavoring agents include, but are not limited to, menthol, peppermint, such as peppermint and spearmint, chocolate, licorice, citrus and other fruit flavors, gamma octalactone, vanillin, ethyl vanillin, breath freshener flavors, spices, such as cinnamon, methyl salicylate, linalool, eugenol, bergamot oil, geranium oil, lemon oil and tobacco flavor. Other suitable flavors may include flavor compounds selected from acids, alcohols, esters, aldehydes, ketones, pyrazines, combinations or blends thereof, and the like.

In certain embodiments of the invention, the aerosol modifier may be an essential oil derived from one or more plants. For example, the homogenized oregano material may comprise oregano oil, such as oregano essential oil, to further enhance the oregano flavor delivered to the consumer upon heating.

In certain embodiments of the invention, the aerosol-generating substrate may comprise homogenized oregano material comprising particulate plant material, such as tea particles, in combination with oregano oil.

The aerosol modifier may be an adsorbent material such as activated carbon that removes certain aerosol constituents that pass through the filter and thereby alters the flavor and aroma of the aerosol.

The one or more aerosol-modifying elements may be located downstream of the aerosol-generating substrate or within the aerosol-generating substrate. The aerosol-generating substrate may comprise homogenized oregano material and an aerosol-modifying element. In various embodiments, the aerosol-modifying element may be placed adjacent to or embedded in the homogenized oregano material. Typically, the aerosol-modifying element may be located downstream of the aerosol-generating substrate, most typically within the aerosol-cooling element, within a filter of the aerosol-generating article, such as within a filter-tip segment or within a cavity, preferably within a cavity between filter-tip segments. The one or more aerosol-modifying elements may be in the form of one or more of a wire, a capsule, a microcapsule, a bead, or a polymer matrix material, or a combination thereof.

If the aerosol-modifying element is in the form of A wire, as described in WO-A-2011/060961, the wire may be formed from paper such as A filter-tip segment wrapper, and the wire may be loaded with at least one aerosol-modifying agent and located within the filter body. Other materials that may be used to form the wire include cellulose acetate and cotton.

If the aerosol regulating member is in the form of A capsule, as described in WO-A-2007/010407, WO-A-2013/068100 and WO-A-2014/154887, the capsule may be A breakable capsule located within the filter, the inner core of the capsule containing an aerosol regulator which can be released when the filter is subjected to an external force when the capsule shell breaks. The capsule may be located in the filter segments or in the cavity, preferably in the cavity between the filter segments.

If the aerosol-modifying element is in the form of A polymer matrix material, the polymer matrix material releases the flavouring when the aerosol-generating article is heated, for example when the polymer matrix is heated beyond the melting point of the polymer matrix material, as described in WO-A-2013/034488. Typically, such polymer matrix material may be located within beads within an aerosol-generating substrate. Alternatively or additionally, the flavoring agent may be trapped within the domains of the polymer matrix material and may be released from the polymer matrix material upon compression of the polymer matrix material. Preferably, the flavoring agent is released upon compression of the polymer matrix material with a force of about 15 newtons. Such a flavour modifying element may provide sustained release of liquid flavourant over a force of at least 5 newtons, such as in the range 5N to 20N, as described in WO 2013/068304. Typically, such polymer matrix material may be located within beads within a filter.

The aerosol-generating article may comprise a combustible heat source and an aerosol-generating substrate downstream of the combustible heat source, the aerosol-generating substrate being as described above with respect to the first aspect of the invention.

For example, the substrate as described herein may be used in A heated aerosol-generating article of the type disclosed in WO-A-2009/022232 comprising A combustible carbon-based heat source, an aerosol-generating substrate downstream of the combustible heat source, and A thermally-conductive element surrounding and in contact with A rear portion of the combustible carbon-based heat source and an adjacent front portion of the aerosol-generating substrate. However, it should be understood that the substrates as described herein may also be used in heated aerosol-generating articles comprising combustible heat sources having other configurations.

The present invention provides an aerosol-generating system comprising an aerosol-generating device comprising a heating element, and an aerosol-generating article for use with the aerosol-generating device, the aerosol-generating article comprising an aerosol-generating substrate as described above.

In a preferred embodiment, an aerosol-generating substrate as described herein may be used in a heated aerosol-generating article for use in an electrically operated aerosol-generating system, wherein the aerosol-generating substrate of the heated aerosol-generating article is heated by an electrical heat source.

For example, an aerosol-generating substrate as described herein may be used in a heated aerosol-generating article of the type disclosed in EP-a-0 822 760.

The heating element of such an aerosol-generating device may be of any suitable form to conduct heat. The heating of the aerosol-generating substrate may be effected internally, externally or both internally and externally. The heating element may preferably be a heater blade or pin adapted to be inserted into the substrate such that the substrate is heated from the inside. Or the heating element may partially or completely surround the substrate and circumferentially heat the substrate from the outside.

The aerosol-generating system may be an electrically operated aerosol-generating system comprising an induction heating device. The induction heating device typically comprises an induction source configured to be coupled with a susceptor, which may be arranged outside the aerosol-generating substrate or inside the aerosol-generating substrate. The induction source generates an alternating electromagnetic field that induces magnetization or eddy currents in the susceptor. Susceptors may be heated due to hysteresis losses or induced eddy currents that heat the susceptor by ohmic or resistive heating.

An electrically operated aerosol-generating system comprising an induction heating device may further comprise an aerosol-generating article having an aerosol-generating substrate and a susceptor in thermal proximity to the aerosol-generating substrate. Typically, the susceptor is in direct contact with the aerosol-generating substrate and heat is transferred from the susceptor to the aerosol-generating substrate primarily by conduction. Examples of electrically operated aerosol-generating systems with induction heating means and aerosol-generating articles with susceptors are described in WO-A1-95/27411 and WO-A1-2015/177255.

The susceptor may be a plurality of susceptor particles, which may be deposited on or embedded within the aerosol-generating substrate. When the aerosol-generating substrate is in the form of one or more sheets, the plurality of susceptor particles may be deposited on or embedded within the one or more sheets. The susceptor particles are fixed by a matrix, for example in the form of a sheet, and remain in the initial position. Preferably, the susceptor particles may be uniformly distributed in the homogenized oregano material of the aerosol-generating substrate. Due to the particulate nature of the susceptor, heat is generated according to the distribution of particles in the homogenized sheet of oregano material of the substrate. Alternatively, one or more susceptors in the form of sheets, strips, fragments or strips may be placed beside or embedded in the homogenized oregano material. In one embodiment, the aerosol-forming substrate comprises one or more susceptor strips. For example, the strip of aerosol-generating substrate may comprise elongate susceptor elements extending longitudinally therethrough. In another embodiment, the susceptor is present in an aerosol-generating device.

Susceptors may have a heat loss of greater than 0.05 joules/kg, preferably greater than 0.1 joules/kg. Heat loss is the ability of the susceptor to transfer heat to the surrounding material. Because the susceptor particles are preferably uniformly distributed in the aerosol-generating substrate, uniform heat loss from the susceptor particles may be achieved, thus generating a uniform heat distribution in the aerosol-generating substrate and resulting in a uniform temperature distribution in the aerosol-generating article. It has been found that a specific minimum heat loss of 0.05 joules/kg in the susceptor particles allows heating the aerosol-generating substrate to a substantially uniform temperature, thereby providing aerosol generation. Preferably, in such embodiments, the average temperature achieved within the aerosol-generating substrate is from about 200 degrees celsius to about 240 degrees celsius.

Reducing the risk of overheating the aerosol-generating substrate may be supported by using susceptor materials having curie temperatures, which allow a process of heating to only a certain maximum temperature due to hysteresis losses. The susceptor may have a curie temperature of between about 200 degrees celsius and about 450 degrees celsius, preferably between about 240 degrees celsius and about 400 degrees celsius, for example about 280 degrees celsius. When the susceptor material reaches its curie temperature, the magnetic properties change. At the curie temperature, the susceptor material changes from a ferromagnetic phase to a paramagnetic phase. At this time, heating based on energy loss is stopped due to orientation of the ferromagnetic domains. In addition, the heating is then mainly based on vortex formation, so that the heating process automatically weakens when the curie temperature of the susceptor material is reached. Preferably, the susceptor material and its curie temperature are adapted to the composition of the aerosol-generating substrate in order to achieve an optimal temperature and temperature distribution in the aerosol-generating substrate for optimal aerosol generation.

In some preferred embodiments of the aerosol-generating article according to the invention, the susceptor is made of ferrite. Ferrites are ferromagnetic bodies having a high magnetic permeability and are particularly suitable as susceptor materials. The main component of ferrite is iron. Other metal components, such as zinc, nickel, manganese or non-metallic components such as silicon, may be present in varying amounts. Ferrites are relatively inexpensive commercially available materials. The ferrite may be obtained in the form of particles, the size range of which is the size range of particles in the particulate plant material used to form the homogenized plant material according to the invention. Preferably, the particles are fully sintered ferrite powders such as FP160, FP215, FP350 manufactured by PPT, indiana, usa.

In certain embodiments of the invention, an aerosol-generating system comprises an aerosol-generating article comprising an aerosol-generating substrate as defined above, an aerosol-former source and means for evaporating the aerosol-former, preferably a heating element as described above. The aerosol-former source may be a refillable or replaceable reservoir located on the aerosol-generating device. When the reservoir is physically separated from the aerosol-generating article, the generated vapor is directed through the aerosol-generating article. The vapor is contacted with an aerosol-generating substrate that releases volatile compounds such as nicotine and flavoring in particulate plant material to form an aerosol. Optionally, to assist in volatilisation of compounds in the aerosol-generating substrate, the aerosol-generating system may further comprise a heating element to heat the aerosol-generating substrate, preferably in a coordinated manner with the aerosol-former. However, in certain embodiments, the heating element for heating the aerosol-generating article is separate from the heater for heating the aerosol-forming agent.

The invention also provides an aerosol produced upon heating an aerosol-generating substrate, as defined above, wherein the aerosol comprises a specific amount and ratio of a characteristic compound derived from oregano particles as defined above.

According to the invention, the aerosol comprises: ursolic acid in an amount of at least 0.04 micrograms per puff of aerosol; an amount of at least 0.01 micrograms of isothymol per puff of aerosol; and an amount of at least 0.01 micrograms of thymoquinone per puff of aerosol, wherein a puff of aerosol has a volume of 55 milliliters as generated by a smoking machine. For the purposes of the present invention, "puff" is defined as the volume of aerosol released from the aerosol-generating substrate upon heating and collected for analysis, wherein the puff of aerosol has a puff volume of 55 milliliters as generated by a smoking machine. Thus, any reference herein to aerosol "puff" should be understood to refer to 55 milliliters of puff unless otherwise indicated.

The indicated ranges define the total amount of each component measured in a 55 ml aerosol puff. The aerosol may be generated from the aerosol-generating substrate using any suitable means and may be captured and analysed as described above in order to identify and measure the amount of a characteristic compound within the aerosol. For example, a "puff" may correspond to a 55 milliliter puff on a smoking machine, such as that used in the HEALTH CANADA test method described herein.

Preferably, the aerosol according to the present invention comprises at least about 0.1 microgram of ursolic acid per puff of aerosol, more preferably at least about 0.5 microgram of ursolic acid per puff of aerosol. Alternatively or additionally, the aerosol generated by the aerosol-generating substrate comprises at most about 5 micrograms of ursolic acid per puff of aerosol, preferably at most about 4 micrograms of ursolic acid per puff of aerosol, more preferably at most about 3 micrograms of ursolic acid per puff of aerosol. For example, an aerosol generated from an aerosol-generating substrate may comprise about 0.04 microgram to about 5 microgram of ursolic acid per puff of aerosol, or about 0.1 microgram of ursolic acid per puff of aerosol to about 4 microgram of ursolic acid per puff of aerosol, or about 0.5 microgram to about 3 microgram of ursolic acid per puff of aerosol.

Preferably, the aerosol according to the invention comprises at least about 0.05 micrograms of isothymol per puff of aerosol, more preferably at least about 0.2 micrograms of isothymol per puff of aerosol. Alternatively or additionally, the aerosol generated by the aerosol-generating substrate preferably comprises at most about 1.25 micrograms of isothymol per puff of aerosol, more preferably at most about 1 microgram of isothymol per puff of aerosol, even more preferably at most about 0.75 micrograms of isothymol per puff of aerosol. For example, an aerosol generated from an aerosol-generating substrate can comprise from about 0.01 micrograms to about 1.25 micrograms of isothymol per puff of aerosol, or from about 0.05 micrograms to about 1 microgram of isothymol per puff of aerosol, or from about 0.2 micrograms to about 0.75 micrograms of isothymol per puff of aerosol.

Preferably, the aerosol according to the present invention comprises at least about 0.05 micrograms of thymoquinone per puff of aerosol, more preferably at least about 0.1 micrograms of thymohydroquinone per puff of aerosol. Alternatively or additionally, the aerosol generated from the aerosol-generating substrate preferably comprises at most about 1.25 micrograms of thymoquinone per puff of aerosol, more preferably at most about 1 microgram of thymohydroquinone per puff of aerosol, even more preferably at most about 0.75 micrograms of thymohydroquinone per puff of aerosol. For example, an aerosol generated from an aerosol-generating substrate may comprise from about 0.01 microgram to about 1.25 microgram of thymoquinone per puff of aerosol, or from about 0.05 microgram to about 1 microgram of thymohydroquinone per puff of aerosol, or from about 0.2 microgram to about 0.75 microgram of thymohydroquinone per puff of aerosol.

According to the invention, the aerosol composition is such that the amount of ursolic acid per puff of aerosol is preferably at least 2 times the amount of isothymol per puff of aerosol. Thus, the ratio of ursolic acid to isothymol in the aerosol is preferably at least about 2:1. Preferably, the aerosol composition is such that the amount of ursolic acid per puff of aerosol is at least three times the amount of isothymol per puff of aerosol.

According to the invention, the aerosol composition is such that the amount of ursolic acid per puff of aerosol is preferably at least 2 times the amount of bairimhydroquinone per puff of aerosol. Thus, the ratio of ursolic acid to bai hydroquinone in the aerosol is preferably at least about 2:1. Preferably, the aerosol composition is such that the amount of ursolic acid per puff of aerosol is at least 3 times the amount of bairimhydroquinone per puff of aerosol.

The defined ratio of ursolic acid to isothymol and thymol hydroquinone characterizes aerosols derived from oregano particles. In contrast, in aerosols produced from oregano essential oils, the ratio of isothymol to ursolic acid and thymol hydroquinone will be significantly different.

Preferably, the aerosol according to the present invention further comprises at least about 0.1 mg of aerosol former per puff of aerosol, more preferably at least about 0.2 mg of aerosol per puff of aerosol, more preferably at least about 0.3 mg of aerosol former per puff of aerosol. Preferably, the aerosol comprises at most 0.6 mg of aerosol former per puff of aerosol, more preferably at most 0.5 mg of aerosol former per puff of aerosol, more preferably at most 0.4 mg of aerosol former per puff of aerosol. For example, the aerosol may comprise from about 0.1 mg to about 0.6 mg of aerosol former per puff of aerosol, or from about 0.2 mg to about 0.5 mg of aerosol former per puff of aerosol, or from about 0.3 mg to about 0.4 mg of aerosol former per puff of aerosol. These values are based on a suction volume of 55 ml as defined above.

Suitable aerosol formers for use in the present invention are described above.

Preferably, the aerosol produced by the aerosol-generating substrate according to the invention further comprises at least about 2 micrograms of nicotine per puff of aerosol, more preferably at least about 20 micrograms of nicotine per puff of aerosol, more preferably at least about 40 micrograms of nicotine per puff of aerosol. Preferably, the aerosol comprises at most about 200 micrograms of nicotine per puff of aerosol, more preferably at most about 150 micrograms of nicotine per puff of aerosol, more preferably at most about 75 micrograms of nicotine per puff of aerosol. For example, the aerosol may comprise about 2 micrograms to about 200 micrograms of nicotine per puff of aerosol, or about 20 micrograms to about 150 micrograms of nicotine per puff of aerosol, or about 40 micrograms to about 75 micrograms of nicotine per puff of aerosol. These values are based on a suction volume of 55 ml as defined above. In some embodiments of the invention, the aerosol may contain zero micrograms of nicotine.

Carbon monoxide may also be present in the aerosols according to the invention and may be measured and used to further characterize the aerosols. Nitrogen oxides such as nitric oxide and nitrogen dioxide may also be present in the aerosol and may be measured and used to further characterize the aerosol.

The aerosols of the invention comprising the characteristic compounds from oregano particles may be formed from particles having a Mass Median Aerodynamic Diameter (MMAD) of about 0.01 to 200 microns or about 1 to 100 microns. Preferably, when the aerosol comprises nicotine as described above, the aerosol comprises particles having MMAD in the range of about 0.1 to about 3 microns, in order to optimize delivery of nicotine from the aerosol.

Mass Median Aerodynamic Diameter (MMAD) of an aerosol refers to the aerodynamic diameter of one half of the mass of particles of the aerosol contributed by particles having an aerodynamic diameter greater than MMAD and one half of the mass of particles contributed by particles having an aerodynamic diameter less than MMAD. The aerodynamic diameter is defined as the diameter of a spherical particle having a density of 1g/cm ³, which particle has the same sedimentation velocity as the characterized particle.

The mass median aerodynamic diameter of an aerosol according to the invention can be determined according to Schaller et al section 2.8, "Evaluation of the Tobacco HEATING SYSTEM 2.2.2. Section 2, ：Chemical composition,genotoxicity,cytotoxicity and physical properties of the aerosol,"Regul.Toxicol.and Pharmacol.,81(2016)S27-S47.

As defined above, the present invention also provides an aerosol-generating article comprising an aerosol-generating substrate comprising a homogenized oregano material, wherein the aerosol generated from the aerosol-generating substrate upon heating the aerosol-generating substrate according to test method a comprises: ursolic acid in an amount of at least 0.04 micrograms per puff of aerosol; an amount of at least 0.01 micrograms of isothymol per puff of aerosol; and an amount of at least 0.01 micrograms of thymoquinone per puff of aerosol, wherein a puff of aerosol has a volume of 55 milliliters as generated by a smoking machine.

For the purposes of the present invention, "puff" is defined as the volume of aerosol released from the aerosol-generating substrate upon heating and collected for analysis, wherein the puff of aerosol has a puff volume of 55 milliliters as generated by a smoking machine. Thus, any reference herein to aerosol "puff" should be understood to refer to 55 milliliters of puff unless otherwise indicated. The indicated ranges define the total amount of each component measured in a 55 ml aerosol puff. The aerosol may be generated from the aerosol-generating substrate using any suitable means and may be captured and analysed as described above in order to identify and measure the amount of a characteristic compound within the aerosol. For example, a "puff" may correspond to a 55 milliliter puff on a smoking machine, such as that used in the HEALTH CANADA test method described herein.

Preferably, the amount of ursolic acid per puff of aerosol is at least 2 times the amount of isothymol per puff of aerosol, more preferably at least 3 times the amount of isothymol per puff of aerosol.

Preferably, the amount of ursolic acid per puff of aerosol is at least 2 times the amount of thymoquinone per puff of aerosol, more preferably at least 3 times the amount of thymohydroquinone per puff of aerosol.

As defined above, the present invention also provides an aerosol-generating substrate formed from homogenized plant material comprising oregano particles, an aerosol-former and a binder, wherein said aerosol-generating substrate comprises: at least 50 micrograms ursolic acid per gram of substrate on a dry weight basis; at least 5 micrograms of isothymol per gram of substrate on a dry weight basis; and at least 1.5 micrograms of bailhydroquinone per gram of substrate on a dry weight basis.

A non-exhaustive list of non-limiting examples is provided below. Any one or more features of these examples may be combined with any one or more features of another example, embodiment, or aspect described herein.

Ex1 an aerosol-generating article comprising an aerosol-generating substrate comprising a homogenized oregano material comprising oregano particles, an aerosol-former, and a binder, wherein the aerosol-generating substrate comprises:

at least 50 micrograms ursolic acid per gram of the matrix on a dry weight basis;

at least 5 micrograms of isothymol per gram of said matrix on a dry weight basis; and

At least 1.5 micrograms of bailhydroquinone per gram of the substrate on a dry weight basis.

Ex2 an aerosol-generating article according to example EX1 wherein the amount of ursolic acid per gram of substrate is at least 8 times the amount of isothymol per gram of substrate.

Ex3 an aerosol-generating article according to example EX1 or EX2 wherein the amount of ursolic acid per gram of substrate is at least 25 times the amount of bairimhydroquinone per gram of substrate.

Ex4 an aerosol-generating article according to example EX1, EX2 or EX3 wherein the aerosol-generating substrate comprises 50 micrograms to 6000 micrograms of ursolic acid per gram of substrate on a dry weight basis.

Ex5 the aerosol-generating article of any of examples EX1 to EX4, wherein the aerosol-generating substrate comprises 5 micrograms to 600 micrograms of isothymol per gram of substrate on a dry weight basis.

The aerosol-generating article according to any one of examples EX1 to EX5, wherein the aerosol-generating substrate comprises from 1.5 micrograms to 200 micrograms of the bairimhydroquinone per gram of substrate on a dry weight basis.

The aerosol-generating article according to any one of examples EX1 to EX6, wherein upon heating the aerosol-generating substrate according to test method a, the aerosol generated comprises:

at least 1.5 micrograms ursolic acid per gram of the matrix on a dry weight basis;

at least 0.5 micrograms of isothymol per gram of said matrix on a dry weight basis; and

At least 0.5 micrograms of bailhydroquinone per gram of the substrate on a dry weight basis.

Ex8 the aerosol-generating article according to example EX7, wherein upon heating the aerosol-generating substrate according to test method a, the aerosol generated comprises at most 200 micrograms of ursolic acid per gram of substrate on a dry weight basis.

Ex9 an aerosol-generating article according to example EX7 or EX8 wherein upon heating the aerosol-generating substrate according to test method a, the aerosol generated comprises at most 50 micrograms of isothymol per gram of substrate on a dry weight basis.

Ex10 an aerosol-generating article according to example EX7, EX8 or EX9, wherein upon heating the aerosol-generating substrate according to test method a, the aerosol generated comprises at most 50 micrograms of bairimhydroquinone per gram of substrate on a dry weight basis.

Ex11 an aerosol-generating article according to any of examples EX7 to EX10, wherein upon heating the aerosol-generating substrate according to test method a, the aerosol generated comprises zero micrograms of nicotine per gram of substrate.

The aerosol-generating article according to any one of examples EX1 to EX6, wherein upon heating the aerosol-generating substrate in the THS2.2 holder according to the HEALTH CANADA machine smoking regime, the aerosol generated comprises:

The aerosol-generating article of any of examples EX1 to EX12, wherein the homogenized oregano material comprises at least 0.5 weight percent oregano particles on a dry weight basis.

The aerosol-generating article of any of examples EX1 to EX13, wherein the homogenized oregano material comprises at most 50 weight percent oregano particles on a dry weight basis.

Ex15 an aerosol-generating article according to any of examples EX1 to EX14, wherein the homogenized oregano material further comprises at most about 75 weight percent tobacco particles on a dry weight basis.

The aerosol-generating article of any of examples EX1 to EX15, wherein the homogenized oregano material further comprises tobacco particles and wherein the weight ratio of oregano particles to tobacco particles is no greater than 1:4.

Ex17 an aerosol-generating article according to example EX15 or EX16, wherein the homogenized oregano material comprises from 5 wt% to 20 wt% oregano particles and from 55 wt% to 70 wt% tobacco particles on a dry weight basis.

The aerosol-generating article of any one of examples EX1 to EX17, wherein the homogenized oregano material comprises substantially zero nicotine.

The aerosol-generating article according to any one of examples EX1 to EX17, wherein the aerosol-generating substrate further comprises at least 0.1mg of nicotine per gram of substrate on a dry weight basis.

Ex20 an aerosol-generating article according to example EX19 wherein the aerosol-generating substrate comprises from 1 mg to 20 mg nicotine per gram of substrate on a dry weight basis.

Ex21 the aerosol-generating article of any of examples EX1 to EX20, wherein the oregano particles have a D95 value of greater than or equal to about 50 microns to a D95 value of less than or equal to about 400 microns.

Ex22 the aerosol-generating article of any of examples EX1 to EX21, wherein the oregano particles have a D5 value of greater than or equal to about 10 microns to a D5 value of less than or equal to about 50 microns.

Ex23 the aerosol-generating article of any one of examples EX1 to EX22, wherein the oregano particles are purposely milled.

Ex24 an aerosol-generating article according to any of examples EX1 to EX23, wherein 100% of the oregano particles have a diameter of less than or equal to 300 microns.

Ex25 the aerosol-generating article of any of examples EX1 to EX24, wherein the homogenized oregano material comprises at most 75 wt% of particulate plant material comprising oregano particles.

The aerosol-generating article of any of examples EX1 to EX25, wherein the homogenized oregano material has an aerosol former content of 5 wt.% to 30 wt.% on a dry weight basis.

The aerosol-generating article according to any one of examples EX1 to EX26, wherein the binder is selected from: gums such as guar gum, xanthan gum, acacia gum and locust bean gum; cellulosic binders such as hydroxypropyl cellulose, carboxymethyl cellulose, hydroxyethyl cellulose, methyl cellulose, and ethyl cellulose; polysaccharides, such as starch; organic acids such as alginic acid; conjugate base salts of organic acids, such as sodium alginate; agar and pectin; and combinations thereof.

The aerosol-generating article of any of examples EX1 to EX27, wherein the binder comprises guar gum.

Ex29 the aerosol-generating article of any of examples EX1 to EX28, wherein the homogenized oregano material comprises 1 wt% to 10 wt% binder on a dry weight basis.

Ex30 an aerosol-generating article according to any of examples EX1 to EX29, wherein the homogenized oregano material further comprises fibers.

Ex31 the aerosol-generating article of example EX30 wherein the fibers have a length greater than 400 microns.

Ex32 the aerosol-generating article according to example EX30 or EX31, wherein the fibers are present in an amount of from about 2 wt% to about 15 wt% based on the dry weight of the aerosol-generating substrate.

Ex33 an aerosol-generating article according to example EX30 or EX31, wherein the fibers are present in an amount of at least 30 wt% based on the dry weight of the aerosol-generating substrate.

The aerosol-generating article of any of examples EX1 to EX33, wherein the homogenized oregano material comprises oregano particles, about 5 wt% to about 30wt% aerosol former, and about 1wt% to about 10wt% binder on a dry weight basis.

Ex35 the aerosol-generating article of example EX34, wherein the homogenized oregano material further comprises from about 2 weight percent to about 15 weight percent fiber.

Ex36 aerosol-generating article according to example EX34 or EX35 wherein the binder is guar gum.

Ex37 an aerosol-generating article according to any of examples EX1 to EX36, wherein the homogenized oregano material is in the form of one or more sheets.

Ex38 the aerosol-generating article of example EX37, wherein each of the one or more sheets has a thickness of 100 micrometers to 600 micrometers.

Ex39 the aerosol-generating article of example EX38, wherein each of the one or more sheets has a grammage of 100g/m ² to 300g/m ².

Ex40 an aerosol-generating article according to example EX38 or EX39, wherein each of the one or more sheets has a density of 0.3g/cm ³ to 1.3g/cm ³.

Ex41 an aerosol-generating article according to example EX38, EX39 or EX40, wherein each of the one or more sheets has a transverse peak tensile strength of 50N/m to 400N/m.

Ex42 an aerosol-generating article according to any of examples EX38 to EX40, wherein each of the one or more sheets has a longitudinal peak tensile strength of 100N/m to 800N/m.

The aerosol-generating article according to any one of examples EX38 to EX42, wherein the one or more sheets are in the form of one or more aggregated sheets.

Ex44 the aerosol-generating article of any one of examples EX1 to EX36, wherein the homogenized oregano material is in the form of a plurality of thin strips.

Ex45 an aerosol-generating article according to example EX44 wherein the width of the sliver is at least 0.2mm.

EX46 the aerosol-generating article according to example EX44 or EX45, wherein the plurality of strands extend substantially longitudinally along the length of the aerosol-generating substrate in alignment with the longitudinal axis.

Ex47 the aerosol-generating article of example EX44, EX45, or EX46, wherein each of the plurality of strands has a mass/surface area ratio of at least 0.02 mg/square millimeter.

Ex48 the aerosol-generating article of any one of examples EX1 to EX47, wherein the homogenized oregano material in the aerosol-generating substrate is in the form of cast leaves.

Ex49 the aerosol-generating article of any of examples EX1 to EX47, wherein the homogenized oregano material in the aerosol-generating substrate is in the form of oregano paper.

Ex50 an aerosol-generating article according to any of examples EX1 to EX49, wherein the aerosol generated from the aerosol-generating substrate when the aerosol-generating substrate is heated according to test method a comprises:

ursolic acid in an amount of at least 0.04 micrograms per puff of aerosol;

An amount of at least 0.01 micrograms of isothymol per puff of aerosol; and

At least 0.01 microgram of thymoquinone per puff of aerosol,

Wherein the one puff aerosol has a volume of 55 milliliters as generated by the smoking machine, wherein the amount of ursolic acid per puff aerosol is at least 2 times the amount of isothymol per puff aerosol, and wherein the amount of ursolic acid per puff aerosol is at least 2 times the amount of thymol per puff aerosol.

Ex51 an aerosol-generating article comprising an aerosol-generating substrate comprising a homogenized oregano material comprising oregano particles, about 5% to about 30% by weight aerosol former, and about 1% to about 10% by weight binder on a dry weight basis.

Ex52 the aerosol-generating article according to example EX51, wherein the homogenized oregano material further comprises an essential oil, preferably oregano essential oil.

Ex53 an aerosol-generating article according to example EX51 or EX52, wherein the homogenized oregano material further comprises tobacco particles.

The aerosol-generating article of any one of examples EX51 to EX53, wherein the homogenized oregano material comprises at least 0.5 weight percent oregano particles on a dry weight basis.

Ex55 an aerosol-generating substrate comprising a homogenized oregano material comprising oregano particles, an aerosol-former, and a binder, wherein the aerosol-generating substrate comprises:

Ex56. an aerosol-generating system, the aerosol-generating system comprising:

An aerosol-generating device comprising a heating element; and

An aerosol-generating article according to any of examples EX1 to EX 54.

Ex57 the aerosol-generating system of example EX56, wherein the heating element is a heater blade adapted to be inserted into the aerosol-generating substrate.

Ex57 an aerosol produced upon heating an aerosol-generating substrate according to example EX55, the aerosol comprising:

ursolic acid in an amount of at least 0.04 micrograms per puff of aerosol;

An amount of at least 0.01 micrograms of isothymol per puff of aerosol; and

At least 0.01 microgram of thymoquinone per puff of aerosol,

Ex58 a method of manufacturing an aerosol-generating substrate, the method comprising the steps of:

Forming a slurry comprising oregano particles, water, an aerosol former, a binder, and optionally tobacco particles;

Casting or extruding the slurry into the form of a sheet or strand; and

The sheet or sliver is dried at 80 to 160 degrees celsius.

Ex59 the method according to example EX58, wherein the slurry is cast onto a support surface and dried to form a sheet of cast leaves.

Ex60 a method of manufacturing an aerosol-generating substrate, the method comprising the steps of:

forming a dilute suspension comprising oregano particles, water, and optionally tobacco particles;

separating the suspension into an insoluble fraction and a liquid extract;

Forming the insoluble portion into a sheet;

Concentrating the liquid extract and adding the concentrated liquid extract to the sheet to form oregano paper.

Specific embodiments will be further described, by way of example only, with reference to the accompanying drawings, in which:

Fig. 1 shows a first embodiment of a matrix of an aerosol-generating article as described herein;

Fig. 2 shows an aerosol-generating system comprising an aerosol-generating article and an aerosol-generating device comprising an electrical heating element;

Fig. 3 shows an aerosol-generating system comprising an aerosol-generating article and an aerosol-generating device comprising a combustible heating element;

Figures 4a and 4b show a second embodiment of a matrix of an aerosol-generating article as described herein;

fig. 5 shows a third embodiment of a matrix of an aerosol-generating article as described herein;

FIGS. 6a, 6b and 6c each show a cross-sectional view of a filter 1050 further comprising an aerosol-modifying element, wherein

Figure 6a shows an aerosol-modifying element in the form of a spherical capsule or bead within a filter segment.

Figure 6b shows the aerosol-modifying element in the form of a wire within the filter segment.

FIG. 6c shows an aerosol-modifying element in the form of a spherical capsule within a cavity within a filter;

Fig. 7 is a cross-sectional view of a rod of aerosol-generating substrate 1020 additionally comprising an elongated susceptor element; and

Fig. 8 shows an experimental set-up for collecting an aerosol sample to be analyzed for measuring a characteristic compound.

Fig. 1 illustrates a heated aerosol-generating article 1000 comprising a substrate as described herein. The article 1000 comprises four elements: aerosol-generating substrate 1020, hollow cellulose acetate tube 1030, spacer element 1040, and mouthpiece filter 1050. The four elements are arranged sequentially and in coaxial alignment and assembled from a wrapper 1060 to form the aerosol-generating article 1000. The article 1000 has a mouth end 1012 for insertion into his or her mouth by a user during use, and a distal end 1013 located at the end of the article opposite the mouth end 1012. The embodiment of the aerosol-generating article illustrated in fig. 1 is particularly suitable for use with an electrically operated aerosol-generating device comprising a heater for heating an aerosol-generating substrate.

When assembled, the article 1000 has a length of about 45 millimeters and an outer diameter of about 7.2 millimeters and an inner diameter of about 6.9 millimeters.

Aerosol-generating substrate 1020 comprises a rod formed from a sheet of homogenized oregano material comprising oregano particles alone or in combination with tobacco particles.

A number of examples of suitable homogenized oregano materials for forming aerosol-generating substrate 1020 are shown in table 1 below (see samples B-D). The sheets are gathered, crimped and wrapped in filter paper (not shown) to form a strip. The sheet contains additives including glycerin as an aerosol former.

The aerosol-generating article 1000 as shown in fig. 1 is designed to be engaged with an aerosol-generating device in order to be consumed. Such an aerosol-generating device comprises means for heating the aerosol-generating substrate 1020 to a sufficient temperature to form an aerosol. In general, the aerosol-generating device may comprise a heating element surrounding the aerosol-generating article 1000 adjacent to the aerosol-generating substrate 1020, or a heating element inserted into the aerosol-generating substrate 1020.

Once engaged with the aerosol-generating device, a user draws on the mouth end 1012 of the smoking article 1000, and the aerosol-generating substrate 1020 is heated to a temperature of about 375 degrees celsius. At this temperature, volatile compounds are evolved from the aerosol-generating substrate 1020. These compounds condense to form aerosols. The aerosol is drawn through the filter 1050 and into the user's mouth.

Fig. 2 shows a portion of an electrically operated aerosol-generating system 2000 that utilizes a heating blade 2100 to heat an aerosol-generating substrate 1020 of an aerosol-generating article 1000. The heating blade is mounted within the aerosol-product receiving chamber of the electrically operated aerosol-generating device 2010. The aerosol-generating device defines a plurality of air holes 2050 to allow air to flow to the aerosol-generating article 1000. The air flow is indicated by the arrows on fig. 2. The aerosol-generating device comprises a power supply and electronics, which are not shown in fig. 2. The aerosol-generating article 1000 of fig. 2 is as described with respect to fig. 1.

In an alternative configuration shown in fig. 3, the aerosol-generating system is shown with a combustible heating element. While the article 1000 of fig. 1 is intended to be consumed in conjunction with an aerosol-generating device, the article 1001 of fig. 3 includes a combustible heat source 1080 that can be ignited and transfer heat to the aerosol-generating substrate 1020 to form an inhalable aerosol. The combustible heat source 80 is a charcoal element that is assembled proximate to the aerosol-generating substrate at the distal end 13 of the strip 11. Elements that are substantially identical to elements in fig. 1 are given the same reference numerals.

Fig. 4a and 4b illustrate a second embodiment 4000a, 4000b of a heated aerosol-generating article. The aerosol-generating substrates 4020a, 4020b comprise a first downstream rod 4021 formed of particulate plant material comprising oregano particles and a second upstream rod 4022 formed of particulate plant material comprising primarily tobacco particles. Suitable homogenized oregano materials for use in the first downstream rod are shown in table 1 below as one of samples a-D. Suitable homogenized tobacco material for use in the second upstream rod is shown in table 1 below as sample E. Sample E contained only tobacco particles and was included for comparison purposes only.

In each rod, the homogenized plant material is in the form of a sheet that is crimped and wrapped in filter paper (not shown). The sheets each contain additives including glycerin as an aerosol former. In the embodiment shown in fig. 4a, the bars are combined in abutting end-to-end relationship to form a bar, and each bar has an equal length of about 6 mm. In a more preferred embodiment (not shown), the second rod is preferably longer than the first rod, e.g., preferably 2mm long, more preferably 3mm long, such that the second rod is 7 or 7.5mm long and the first rod is 5 or 4.5mm long to provide the desired tobacco to oregano particle ratio in the matrix. In fig. 4b, the cellulose acetate tube support element 1030 is omitted.

Similar to the article 1000 in fig. 1, the articles 4000a, 4000b are particularly suited for use with an electrically operated aerosol-generating system 2000 that includes a heater as shown in fig. 2. Elements in fig. 1 that are substantially the same are given the same reference numerals. Those skilled in the art will appreciate that a combustible heat source (not shown) may alternatively be used in place of the electrical heating element with the second embodiment in a configuration similar to that of article 1001 of fig. 3 that includes combustible heat source 1080.

Fig. 5 illustrates a third embodiment 5000 of the heated aerosol-generating article. The aerosol-generating substrate 5020 comprises a rod formed from a first sheet of homogenized oregano material formed from a particulate plant material comprising a proportion of oregano particles and a second sheet of homogenized tobacco material comprising predominantly cast leaf tobacco.

Suitable homogenized oregano materials for use as the first sheet are shown in table 1 below as one of samples a through D. Suitable homogenized tobacco materials for use as the second sheet are shown in table 1 below as sample E. Sample E contained only tobacco particles and was included for comparison purposes only.

The second sheet is overlaid on top of the first sheet and the combined sheets have been crimped, gathered and at least partially wrapped in filter paper (not shown) to form a rod that is part of a strip. Both sheets contain additives, including glycerin as an aerosol former. Similar to the article 1000 in fig. 1, the article 5000 is particularly suitable for use with an electrically operated aerosol-generating system 2000 that includes a heater as shown in fig. 2. Elements in fig. 1 that are substantially the same are given the same reference numerals. Those skilled in the art will appreciate that a combustible heat source (not shown) may alternatively be used with the third embodiment in place of the electrical heating element in a configuration similar to that of article 1001 of fig. 3 that includes combustible heat source 1080.

Fig. 6a, 6b and 6c are cross-sectional views of a filter 1050 that also includes an aerosol-modifying element. In fig. 6a, filter 1050 also comprises an aerosol-modifying element in the form of a spherical capsule or bead 605.

In the embodiment of fig. 6a, capsules or beads 605 are embedded in the filter segment 601 and surrounded on all sides by the filter material 603. In this embodiment, the capsule comprises an outer shell and an inner core, and the inner core contains a liquid flavoring agent. Liquid flavouring is used to flavour an aerosol during use of an aerosol-generating article provided with a filter. When the filter is subjected to an external force, such as by a consumer, the capsule 605 releases at least a portion of the liquid flavoring. In the embodiment shown, the capsule is generally spherical with a substantially continuous outer shell containing liquid flavoring.

In the embodiment of fig. 6b, the filter segment 601 comprises a rod of filter material 603 and a central flavor bearing line 607 extending in a roll direction through the rod of filter material 603 parallel to the longitudinal axis of the filter 1050. The length of the central flavour carrier line 607 is substantially the same as the length of the filter material rod 603 such that the ends of the central flavour carrier line 607 are visible at the ends of the filter segments 601. In fig. 6b, the filter material 603 is cellulose acetate tow. The central flavour carrier line 607 is formed of a twisted filter segment wrapper and is loaded with aerosol modifiers.

In the embodiment of fig. 6c, the filter segment 601 comprises more than one rod 603, 603' of filter material. Preferably, the filter material rods 603, 603' are formed of cellulose acetate such that they are capable of filtering aerosols provided by the aerosol-generating article. The wrapper 609 wraps around and connects the filter segments 603, 603'. Within the cavity 611 is a capsule 605 comprising an outer shell and an inner core, and the inner core contains a liquid flavoring. The capsule is otherwise similar to the embodiment of fig. 6 a.

Fig. 7 is a cross-sectional view of an aerosol-generating substrate 1020 additionally comprising an elongated susceptor strip 705. Aerosol-generating substrate 1020 comprises rod 703 formed from a sheet of homogenized oregano material comprising tobacco particles and oregano particles. An elongated susceptor strip 705 is embedded within rod 703 and extends in the longitudinal direction between the upstream and downstream ends of rod 703. During use, the elongate susceptor strip 705 heats the homogenized oregano material by means of induction heating as described above.

Examples

As described above with reference to the drawings, different samples of homogenized plant material for aerosol generating substrates according to the invention may be prepared from aqueous slurries having the compositions shown in table 1. Sample a contained only oregano particles and no tobacco particles according to the invention. Samples B through D contained oregano particles and tobacco particles according to the present invention. Sample E contained only tobacco particles and was included for comparison purposes only.

The particulate plant material in all samples a-E accounted for about 75% of the dry weight of the homogenized plant material, and the glycerol, guar gum, and cellulose fibers accounted for the remaining about 25% of the dry weight of the homogenized plant material. The samples were prepared from aqueous slurries containing 78-79kg water per 100kg slurry.

In the following table,% DWB refers to "dry weight basis", in which case the weight percentages are calculated relative to the dry weight of the homogenized plant material. Oregano powder may be formed from dried Oregano leaves, which may be ground to a final D95=175 microns by triple impact milling.

TABLE 1 Dry content of the slurries

The slurry was cast onto a glass plate using a casting bar (0.6 mm), dried in an oven at 140 degrees celsius for 7 minutes, and then dried in a second oven at 120 degrees celsius for 30 seconds.

For each of samples a through E of homogenized plant material, a rod was produced from a single continuous sheet of homogenized plant material, each having a width of 100mm to 125 mm. Each sheet preferably has a thickness of about 235 microns and a grammage of about 180g/m ². The cut width of each sheet was about 132mm. The sheet was crimped to a height of 165 microns to 170 microns and rolled into a rod having a length of about 12mm and a diameter of about 7mm, surrounded by a wrapper. The weight of homogenized plant material in each rod was about 285mg and the total weight of each rod was about 293.6mg.

For each rod, an aerosol-generating article having an overall length of about 45mm may be formed having a structure as shown in fig. 3, comprising, from the downstream end: an oral-end cellulose acetate filter (about 7mm long), an aerosol spacer comprising crimped sheets of polylactic acid polymer (about 18mm long), a hollow cellulose acetate tube (about 8mm long) and a rod of aerosol-generating substrate.

For sample B of homogenized plant material, the oregano particles account for 20% of the granular plant material, and methanol is used to extract the characteristic compounds from the rods of homogenized plant material as described above. The extracts were analyzed as described above to confirm the presence of the characteristic compounds and to measure the amounts of the characteristic compounds. The results of this analysis are shown in table 2 below, where the indicated amounts correspond to the amounts of each aerosol-generating article, where the aerosol-generating substrate of the aerosol-generating article comprises 285mg of sample B of homogenized plant material.

The amount of the characteristic compound present in the granular plant material (oregano granules) used to form sample B is also shown for comparison purposes. For particulate material, the indicated amount corresponds to the amount of the characteristic compound in the sample of particulate plant material having a weight corresponding to the total weight of particulate plant material in the aerosol-generating article containing 285mg of sample B.

For each of samples A, C and D containing a proportion of oregano particles, the amount of the characteristic compound can be estimated based on the values in table 2 by assuming that the amount is proportional to the weight of the oregano particles.

TABLE 2 amount of oregano specific compounds in particulate plant material and aerosol generating substrate

Mainstream aerosols of aerosol-generating articles incorporating aerosol-generating substrates formed from samples a to E of homogenized plant material may be generated according to test method a as defined above. For each sample, the generated aerosol can be captured and analyzed.

As described in detail above, according to test method a, commercially available can be usedThe heat-not burn device tobacco heating system 2.2 holder (THS 2.2 holder) (from Philip Morris Products SA) was used to test aerosol-generating articles. Heating the aerosol-generating article in a HEALTH CANADA machine smoking regime, wherein the puff volume is 55ml, the puff duration is 2 seconds and the puff interval is 30 seconds (as described in ISO/TR 19478-1:2014).

Aerosols generated during the smoking test were collected on a Cambridge filter pad and extracted with a liquid solvent. Fig. 10 shows a suitable device for generating and collecting aerosols from an aerosol-generating article.

The aerosol-generating device 111 shown in fig. 10 is a commercially available tobacco heating device (IQOS). The contents of the mainstream aerosol generated during the HEALTH CANADA smoking test as described above are collected in the aerosol collection chamber 113 on the aerosol collection line 120. The glass fiber filter pad 140 is a 44mm Cambridge glass fiber filter pad (CFP) according to ISO 4387 and ISO 3308.

For LC-HRAM-MS analysis:

The extraction solvent 170, 170a in this case is a methanol and Internal Standard (ISTD) solution having a volume of 10mL in each of the miniature dust-measuring devices 160, 160 a. The cold baths 161, 161a each contain dry ice-isopropyl ether to maintain the micro dust meter 160, 160a at about-60 ℃ each, and the gas-vapor phase is trapped in the extraction solvent 170, 170a as the aerosol bubbles through the micro dust meter 160, 160 a. In step 181, the combined solution from the two miniature dust-measuring devices is separated into a gas-vapor phase solution 180 captured by the dust-measuring devices.

In step 190, the CFP and dust meter captured gas-vapor phase solution 180 is combined in a clean environmentIn the tube. In step 200, the gas-vapor phase solution 180 (which contains methanol as solvent) captured using a dust tester was used to extract total particulate matter from the CFP by sufficient shaking (to disintegrate the CFP), vortexing for 5 minutes and final centrifugation (4500 g,5min,10 ℃). An aliquot (300 μl) of reconstituted whole aerosol extract 220 was transferred to a silanized chromatography vial and diluted with methanol (700 μl) because the extraction solvents 170, 170a already contained an Internal Standard (ISTD) solution. The vials were closed and mixed for 5 minutes using Eppendorf ThermoMixer (5 ℃ C.; 2000 rpm).

Aliquots (1.5 μl) of the diluted extracts were injected and analyzed by LC-HRAM-MS in full scan mode and data dependent fragmentation mode for compound identification.

For GCxGC-TOFMS analysis:

As described above, when preparing GCxGC-TOFMS experimental samples, different solvents are suitable for extracting and analyzing polar compounds, non-polar compounds and volatile compounds separated from the whole aerosol. The experimental setup was the same as described for sample collection of LC-HRAM-MS, except as noted below.

Non-polarity and polarity

Extraction solvents 171, 171a, are present in a volume of 10mL and are an 80:20v/v mixture of dichloromethane and methanol, and further comprise a Retention Index Marker (RIM) compound and a stable isotopically-labeled Internal Standard (ISTD). The cold baths 162, 162a each contain a dry ice-isopropyl alcohol mixture to maintain the micro dust gauge 160, 160a at about-78 ℃ each, the gas-vapor phase being trapped in the extraction solvent 171, 171a as the aerosol bubbles through the micro dust gauge 160, 160 a. In step 182, the combined solution from the two miniature dust meters is separated into a gas-vapor phase solution 210 that is captured by the dust meters.

Nonpolar material

In step 190, the CFP and dust meter captured gas-vapor phase solution 210 is combined in a clean environmentIn the tube. In step 200, the gas-vapor phase solution 210 (which contains methylene chloride and methanol as solvents) captured using a dust tester, was used to extract total particulate matter from the CFP by shaking sufficiently (to disintegrate the CFP), vortexing for 5 minutes, and finally centrifuging (4500 g,5min,10 ℃) to separate the polar and non-polar components of the total aerosol extract 230.

In step 250, a 10mL aliquot 240 of the full aerosol extract 230 is taken. In step 260, a 10mL aliquot of water is added and the entire sample is shaken and centrifuged. The non-polar fraction 270 was separated, dried over sodium sulfate and analyzed by GCxGC-TOFMS in full scan mode.

Polarity of

The ISTD and RIM compounds were added to the polar fraction 280 and then analyzed directly in full scan mode by GCxGC-TOFMS.

Each smoking parallel assay (n=3) contains a cumulative trapped and reconstituted non-polar fraction 270 and polar fraction 280 for each sample

Volatile component

Two miniature dust gauges 160, 160a in series are used to capture the full aerosol. The extraction solvent 172, 172a is in this case N, N-Dimethylformamide (DMF) containing a Retention Index Marker (RIM) compound and a stable isotope-labeled Internal Standard (ISTD), which has a volume of 10mL in each of the miniature dust-measuring devices 160, 160 a. The cold baths 161, 161a each contain dry ice-isopropyl ether to maintain the micro dust meter 160, 160a at about-60 ℃ each, and the gas-vapor phase is trapped in the extraction solvent 170, 170a as the aerosol bubbles through the micro dust meter 160, 160 a. In step 183, the combined solution from the two miniature dust measuring devices is separated into volatile-containing phases 211. The volatile containing phase 211 was analyzed separately from the other phases and injected directly into the GCxGC-TOFMS using on-column cooling injection without further preparation.

Table 3 below shows the content of a characteristic compound from oregano particles in an aerosol generated from an aerosol-generating article (comprising only oregano particles) incorporating homogenized plant material sample B. For comparison purposes, table 3 also shows the levels of characteristic compounds in the aerosol generated from the aerosol-generating article of sample E incorporating homogenized plant material comprising only tobacco particles (and thus not according to the invention).

TABLE 3 content of characteristic Compounds in aerosols

In the aerosol generated from sample B, a relatively high level of the characteristic compound was measured. The ratio of ursolic acid to isothymol is higher than 1 and the ratio of isothymol to thymol is also higher than 8. Thus, the level of the characteristic compound is indicative of the presence of oregano particles in the sample. In contrast, for sample E of tobacco only, which is substantially free of oregano particles, the level of the characteristic compound was found to be zero or near zero.

For each of samples A, C and D containing oregano particles, the amount of the characteristic compound in the aerosol can be estimated based on the values in table 3 by assuming that the amount is proportional to the weight of oregano particles in the aerosol-generating substrate from which the aerosol is generated.

Table 4 below compares the level of certain aerosol constituents in the aerosol generated from the aerosol-generating article incorporating sample B (20:80 ratio of oregano to tobacco) to the aerosol generated from sample E alone. The indicated reduction is the percentage reduction provided by replacing 20% of the tobacco particles in the homogenized material of sample E with oregano particles.

As shown in table 4, the aerosols produced from sample B containing 20 wt% oregano particles based on the dry weight of the particulate plant material resulted in a reduction in the levels of phenol, o-cresol, and m-cresol when compared to the levels of the same compounds in the aerosols produced from sample E containing 100 wt% tobacco based on the dry weight of the particulate plant material.

In most cases, the reduction provided in the level of these undesirable aerosol compounds is significantly greater than the proportional reduction expected from the replacement of 20% of the tobacco particles with oregano particles. Thus, inclusion of a combination of oregano particles with tobacco particles provides unexpectedly high reductions in the levels of these compounds. Thus, the inclusion of oregano particles may provide an aerosol having improved sensory attributes while reducing the level of certain undesirable compounds in the aerosol.

TABLE 4 composition of aerosols

Claims

1. An aerosol-generating article comprising an aerosol-generating substrate comprising a homogenized oregano material comprising oregano particles, an aerosol-forming agent, and a binder, wherein the aerosol-generating substrate comprises:

2. An aerosol-generating article according to claim 1, wherein the amount of ursolic acid per gram of the substrate is at least 8 times the amount of isothymol per gram of the substrate, and wherein the amount of ursolic acid per gram of the substrate is at least 25 times the amount of thymol per gram of the substrate.

3. An aerosol-generating article according to claim 1 or 2, wherein the aerosol-generating substrate further comprises from 1 mg to 20 mg of nicotine per gram of the substrate on a dry weight basis.

4. An aerosol-generating article according to any preceding claim, wherein the homogenized oregano material comprises from 5 wt% to 30 wt% aerosol former and from 1 wt% to 10 wt% binder on a dry weight basis.

5. An aerosol-generating article according to any preceding claim, wherein the binder comprises guar gum.

6. An aerosol-generating article according to any preceding claim, wherein the homogenized oregano material comprises at least 0.5 wt% of the oregano particles on a dry weight basis.

7. An aerosol-generating article according to any preceding claim, wherein the homogenized oregano material further comprises tobacco particles and wherein the weight ratio of oregano particles to tobacco particles is not greater than 1:4.

8. An aerosol-generating article according to any preceding claim, wherein the homogenized oregano material in the aerosol-generating substrate is in the form of cast leaves.

9. An aerosol-generating article according to any one of claims 1 to 7, wherein the homogenized oregano material in the aerosol-generating substrate is in the form of oregano paper.

10. An aerosol-generating article according to any preceding claim, wherein upon heating the aerosol-generating substrate according to test method a, the aerosol generated comprises:

11. An aerosol-generating article according to any preceding claim, wherein an aerosol generated from the aerosol-generating substrate when the aerosol-generating substrate is heated according to test method a comprises:

ursolic acid in an amount of at least 0.04 micrograms per puff of aerosol;

An amount of at least 0.01 micrograms of isothymol per puff of aerosol; and

At least 0.01 microgram of thymoquinone per puff of aerosol,

12. An aerosol-generating substrate comprising a homogenized oregano material comprising oregano particles, an aerosol-forming agent, and a binder, wherein the aerosol-generating substrate comprises:

13. An aerosol-generating system, the aerosol-generating system comprising:

An aerosol-generating device comprising a heating element; and

An aerosol-generating article according to any one of claims 1 to 12.

14. An aerosol generated upon heating an aerosol-generating substrate according to claim 12, the aerosol comprising:

ursolic acid in an amount of at least 0.04 micrograms per puff of aerosol;

An amount of at least 0.01 micrograms of isothymol per puff of aerosol; and

At least 0.01 microgram of thymoquinone per puff of aerosol,

15. A method of manufacturing an aerosol-generating substrate, the method comprising the steps of:

Casting or extruding the slurry into the form of a sheet or strand; and

The sheet or sliver is dried at 80 to 160 degrees celsius.